BioWave Pro 4pg - Ted Pella, Inc.

PELCO BioWave®ProNext level of microwave tissue processingSuperior results from microwave to microscope

Controlling the PELCO BioWave ® Pro has never been easier.From the main menu four clear choices give the operator access to levels of control not availablein the past. Main controls are:• Manual Control• Automatic Protocols• Presets• System OptionsManual Control takes the user to the main screen control for manual operation where all importantparameters can be viewed and enteredAutomatic Protocols takes the user directly to stored protocols where a single touch of a buttonruns a preprogrammed set of microwave processing parameters. In this way power, temperature,time and vacuum are run at preset parameters saving time, reducing errors andincreasing productivity. A number of protocols are factory stored, custom protocols can beentered and managed with a set of protocol management tools.Presets takes the user to 10 preset processing configurations that ensure repetitive steps aredone easily without having to enter parameters each time.System Options facilitates easy and precise control over ancillary systems that support thebeneficial effects of controlled microwave radiation:• Integrated cooler• Vacuum level and delivery• Stirring rate of the stirrer• PELCO SteadyTemp settingsThe intuitive controlling system is designed to use the appropriate microwave power outputfor the process at hand, delivering the best results for a multitude of applications. Full andeasy control enables succesful and consistent processing results.TED PELLA, INC.

#36500 PELCO BioWave ® Pro - Standard SystemIncludes:Pelgraph SoftwareVariety of Stir BarsTemperature ProbeTemperature Probe StandThe PELCO BioWave ® Pro can be readily configured for specificapplications with our application kits:#36500-10 EM Tissue Processing Application Kit36115 PELCO ColdSpot ®3435 Microwave Vacuum Chamber050-010 Bubbler Probe36131 Microwave Capsule Holder36134 Microwave Microcentrifuge Tube Holder36133 Microwave Polymerization Tray36157 Prep-Eze 6-Well Kit36158 Prep-Eze 12-Well Kit#36500-20 Immunolabeling Application Kit36115 PELCO ColdSpot ®3435 Microwave Vacuum Chamber050-010 Bubbler Probe36105 Sequenza Slide rack36107 Coverplate Assemblies36170 12-Wellplate insert36172 24-Wellplate insert#36500-30 Fixation/Decalcification Kit50051 PELCO SteadyTemp 36200 DFR Insert with PTFE cassette rack50050-10 Vapor Trap#36500-40 Paraffin Processing Kit36115 PELCO ColdSpot ®36160 PELCO TissueVac ®050-010 Bubbler Probe36150 PELCO HistoWave ® Cassette System36150-1 Paraffin Heating Tile for 36150#36500-50 Confocal In situApplication Kit50051 PELCO SteadyTemp 36115 PELCO ColdSpot ®3435 Microwave Vacuum Chamber36170 12-Wellplate insert36172 24-Wellplate insertTED PELLA, INC.Microscopy Products for Science and IndustryP. O. Box 492477 Redding, CA 96049-2477Phone: 530-243-2200 or 800-237-3526 (USA) FAX: 530-243-3761Email: sales@tedpella.com Web Site: www.tedpella.comFigure 1Retinas were fixed in 4% paraformaldehyde in 0.1M phosphate buffer (pH 7.4) overnight at4°C. Following labeling the tissue was rinsed 6 x 20 minutes in buffer prior to beginning antibodylabeling. The bench labeling protocol requires 7 days. These labeling results were completedin an afternoon using microwave-enhanced labeling (PELCO BioWave ® withSteadyTemp ) during a workshop held at the Univ. of Minnesota Imaging Center (MarkSanders, Director - May 17-19, 2006). The retinas were double-labeled for:• Collagen Type IV (basal lamina surrounding blood vessels) with rabbit anti-type IV collagenand the secondary conjugated to FITC (green label)• Glutamine Synthetase (enzyme found in retinal Müller glial cells) with mouse anti- glutaminesynthetase and the secondary conjugated to Cy3 (red label)Primary antibody labeling was done at 170W for 12 minutes (4 on - 4 off - 4 on) under vacuum(15" Hg). Secondary antibody labeling was done at 170W for 6 minutes (2 on - 2 off - 2 on)under vacuum (15" Hg). Images were collected on a Nikon C1si Confocal Microscope.PELCO BioWave ® Pro System SpecificationsMicrowave power range Continuous power settings from 90-750 WattsMicrowave frequency 2.45 GhzMicrowave power control Programmable controller with 10 modifiablepresetsFunction control6" Touch screen user interfaceTemperature control ± 1°C for most aqueous solutionsCooling internalIntegrated ambient water cooling systemCooling external (optional) PELCO SteadyTemp : 450W chilled coolingsystemMagnetic stirrerIntegrated, 0-300rpm speedExhaust110cfm capacityVentingAutomatic when door is openedVacuum system20" Hg, 3 selectable modesAir bubblerUp to 0.8ltr/min with 2.5" column of waterpressureProtocol management Protocols can be stored, using a total of 200stepsCertificationUL/CEDimensions55.3 W x 51.4 D x 54.6 H cm(21.75 x 20.25 x 21.5")Weight37.7 kg (83 lbs)Power required15A/115VACPELCO, PELCO BioWave and PELCO ColdSpot are registered trademarks. All Rights Reserved.SteadyTemp and PELCOTissueVac are trademarks ofTed Pella, Inc. All Rights Reserved.©Ted Pella, Inc., Printed in USA 09-06.