DPO -15Goat Milk – A Future Health FoodP.K.Rout, Ajay Kumar, R. RoyCentral Institute for Research on Goats, Makhdoom – 281 122 (UP)Goat milk has significance in human nutrition. The present study was initiated in Indian goats tocharacterize them both at protein and genomic level and to study the milk proteomics for furthercommercial application. The as 1-casein A allele was observed in the majority <strong>of</strong> goats and theirfrequency in Jamunapari, Barbari, Marwari, Sirohi, Jakhrana, Beetal and non-descript goat from U.Pand M.P was 0.71, 0.77, 0.56, 0.76, 0.67, 0.72, 0.45, 0.58 and 0.52, respectively. In as 1-casein locus,the A variant had highest frequency in all the breeds, however Marwari, Ganjam and non-descriptgoats from U.P and M.P had moderate gene frequency <strong>of</strong> 0.564, 0.450, 0.586 and 0.525,respectively. Barbari had highest and Ganjam goats had lowest as 1-Cn A gene frequency. DNA samples<strong>of</strong> Indian goats were analyzed for the presence <strong>of</strong> different as 1-casein allele. AA genotype hadhighest frequency in Indian goats. The molecular analysis at DNA level revealed mainly A, B, C, D, Eand F allele at CSN1S1 locus. The analysis indicated that the Indian goat breeds are carrying A allelein higher frequency except Ganjam and local goats indicating the availability <strong>of</strong> high amount <strong>of</strong>protein in milk <strong>of</strong> Indian goats.DPO -16Effect <strong>of</strong> Individual or Group Culture onDevelopment <strong>of</strong> Buffalo Preantral FolliclesH.S. Ramesh*, P.S.P. Gupta**, J.P. Ravindra** and V. Chandrashekar Murthy** KVAFSU, Veterinary College, Bangalore – 560 024**National Institute <strong>of</strong> Animal Nutrition and Physiology, Bangalore – 560 030Buffaloes are important dairy animals in India. However one <strong>of</strong> the constraints in exploiting thefull potential is their inherent reproductive problems. Assisted reproductive technologies can be adoptedto overcome the problems. Efforts are being made to utilize preantral follicles in the ovaries forembryo resource generation. Present study attempted to investigate the effect <strong>of</strong> individual and groupculture on in vitro development <strong>of</strong> large preantral follicles in buffaloes. Large preantral follicles <strong>of</strong>150 to 500 mm size isolated by micro-dissection method from slaughter house derived ovaries wereplaced individually or in groups in minimum essential medium (MEM) with supplements and culturedin a CO 2incubator for 80 days. There was no significant difference (P>0.05) in the size <strong>of</strong> folliclesbetween the two groups. However, the preantral follicles in-group culture showed a significantly higher(P
DPO -17Effect <strong>of</strong> Feeding Two Levels <strong>of</strong> Energy andBypass Protein on Production Performance<strong>of</strong> Lactating Crossbred GoatsMieso Guru and Shiv PrasadDairy <strong>Cattle</strong> <strong>Breed</strong>ing Division, NDRI, Karnal – 132 001 (Haryana)An experiment was conducted on 20 lactating crossbred does in early lactation duringJanuary – March 2002. The treatments consisted <strong>of</strong> (i) medium concentrate (@3% <strong>of</strong> BW) with lowby pass protein (RDP:UDP; 69:31) (T 1), (ii) high concentrate (@4% <strong>of</strong> body weight) with low bypassprotein (T 2), (iii) medium concentrate (3% <strong>of</strong> BW) with high by pass protein (RDP : UDP; 52:48) (T 3)and high concentrate (4% <strong>of</strong> BW) with high bypass protein (T 4). The overall means for 4% FCM yieldin the treatment groups T 1, T 2, T 3and T 4were; 1.18 + 0.03, 1.60+ 0.03, 1.75 + 0.03 and 1.95 + 0.04kg per doe per day respectively. The overall FCM yield / kg W 0.7 averaged 0.10+ 0.001, 0.13+ 0.02,0.014 + 0.02 and 0.16 + 0.003 kg respectively in the respective treatment groups. Milk yield/doe/d,4% FCM yield/doe/d as well as FCM yield/kgW 0.07 all were highest in treatment group T 4the treatmenteffect was significant (P