Cortisol Enzyme Immunoassay Kit - Enzo Life Sciences

Calculation of ResultsSeveral options are available for the calculation of the concentration of Cortisol in the samples. Werecommend that the data be handled by an immunoassay software package utilizing a 4 parameterlogistic curve fitting program. If this sort of data reduction software is not readily available, theconcentration of Cortisol can be calculated as follows:1. Calculate the average net Optical Density (OD) bound for each standard and sample bysubtracting the average NSB OD from the average OD bound:Average Net OD = Average Bound OD - Average NSB OD2. Calculate the binding of each pair of standard wells as a percentage of the maximum bindingwells (Bo), using the following formula:Percent Bound = Net OD x 100Net Bo OD3. Using Logit-Log paper plot Percent Bound versus Concentration of Cortisol for the standards.Approximate a straight line through the points. The concentration of Cortisol in theunknowns can be determined by interpolation.Typical ResultsThe results shown below are for illustration only and should not be used to calculate results fromanother assay.Mean Average Percent CortisolSample OD (-Blank) Net OD Bound (pg/mL)Blank OD (0.077)TA 0.307NSB 0.001 0.000 0.0Bo 0.816 0.815 100% 0S1 0.105 0.104 12.9% 10,000S2 0.158 0.157 19.3% 5,000S3 0.248 0.247 30.4% 2,500S4 0.365 0.364 44.7% 1,250S5 0.482 0.481 59.1% 625S6 0.588 0.587 72.1% 313S7 0.676 0.675 82.9% 156Unknown 1 0.260 0.259 31.8% 2,311Unknown 2 0.462 0.461 56.6% 702