hematological values of gyr-saker hybrid falcons and

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The hematological parameters of 689 clinically healthy hybrid falcons wereevaluated. All falcons that were showing clinical signs or that were diagnosed withdisease were excluded from in this study. The complete blood count (CBC) of 369Gyr-Saker hybrid falcons and 320 Gyr-Peregrine hybrid falcons has been examinedmanually. After taking blood from the right or left basilica vein (Vena cutanea ulnarissuperficialis) or the right or left caudal tibial vein (Vena metatarsalis plantarissuperficialis), 0.5ml blood was stored in a 1.0 ml EDTA tube (Teklab). The bloodcount was performed directly after blood taking with a maximum delay time of 30minutes.2.2 MethodRed blood cell count (RBC) (SAMOUR et al 1996)The working solution consists of 10 ml 40% formaldehyde, 31.3 g trisodium citrateand 1000 ml distilled water. 4 ml of this solution has been mixed in a plain sampletube with 20µl of the falcon blood stored in the EDTA tube. This diluted sample hasbeen filled via a capillary tube in the improved Neubauer haemocytometer. After 5minutes waiting time, the cells of 5x16 squares have been counted in the center ofthe counting grid. The counted cells had been calculated as follows:N=Number of cells counted, then: N = RBC x 10 12 / l100White blood cell count (WBC) (SAMOUR et al 1996)1.9 ml of a 1% ammonium oxalate solution had been mixed with 100 µl of the falconblood sample and kept on a tube roller for 3 minutes. A small amount of the dilutedsamples has been filled via a capillary tube in the improved Neubauerhaemocytometer. After 5 minutes waiting time, the cells of 4 outer large squareshave been counted in the center of the counting grid. The counted cells had beencalculated as follows:N=Number of cells counted, then: N = WBC x 10 9 / l20Packed cell volume (PCV)/ Haematocrit (Hct) (SAMOUR et al 1996)The PCV is measured by the used of microhaematocrit capillary tubes (Fortuna,Germany) and centrifuge (Hawsley, UK). In our study, the hematocrit is used as thismight be the more accurate than the PCV (FUDGE, 2000)Mean cell volume: PCV x 10 = MCV(fl)RBCMean cell haemoglobin: Hb x 10 = MCH (pg)RBCMean cell haemoglobin concentration: Hb x 100 = MCHC (g/l)PCVHemoglobinThe hemoglobin is measured by capillary, venous or arterial blood used in EDTA.The optical eye of the Hemocue Microcuvette contains reagents deposited on itsinner wall and the blood sample is drawn in to the cavity by capillary actionspontaneously mixed with the reagents. Used with the HemoCue photometer, thesystem provides a direct reading of the concentration of hemoglobin in a bloodsample.Preparation of blood smearTake one small drop of blood sample in a clean microscopic slide. Position aspreader slide in front of the drop of blood at an angle of about 45° and move the2
spreader backwards and touch gently the drop of blood which will run across theedge of the slide. Push the spreader with a steady forward movement to create ablood smear.Staining method. The HEMAstain test is for the rapid, differential staining ofhematological smears that yields qualitative results similar to Wright-Giemsa stain.The slide is dipped in fixative solution for 5 seconds, than in solution.1 for 5 secondsand than in solution 11 for 5 seconds. The excess solution is allowed to drain. Theslide is rinsed with distilled or deionized water, then allowed to dry and examinedunder oil immersion lens.The examination of the blood smear for the shape of the blood cells and avian bloodparasites is routinely performed for each blood smear, but is not part of this study.3. ResultsThe evaluation of the 369 Falco rusticolus x Falco cherrug hybrid falcons showed thefollowing picture:Table 1: Hematological parameters for Gyr-Saker hybrid falcons (Falco rusticolus xFalco cherrug)Parameters n=369 rangeRBC (x10 12 /l) 2.33±0.15 2.18-2.48HB (g/dl) 17.73±1.50 16.23-19.23Hct % 51.57±4.66 46.91-56.23MCV (fl) 221.85±21.64 200.29-243.49MCH (pg) 76.24±6.83 69.41-83.07MCHC (g/dl) 34.42±1.57 32.85-35.99WBC (x 10 9 /l) 7.50±2.22 5.28-9.72Heterophils % 49.90±3.88 46.02-53.78Lymphocytes % 44.11±2.91 41.20-47.02Monocytes % 4.42±1.58 2.84-6.00Eosinophils % 1.29±0.92 0.37-2.21Basophils % 0.40±0.15 0.25-0.55The 320 evaluated Falco rusticolus x Falco peregrinus led to those results:Table 2: Hematological parameters for Gyr-Peregrine hybrid falcons (Falco rusticolusx Falco peregrinus)Parameters n=320 rangeRBC (x10 12 /l) 2.39±0.26 2.13-2.65HB (g/dl) 17.9±1.57 16.33-19.47Hct % 52.00±4.75 47.25-56.75MCV (fl) 219.7±25.38 194.32-245.08MCH (pg) 75.59±8.45 67.14-84.04MCHC (g/dl) 34.43±1.48 32.95-35.91WBC (x 10 9 /l) 7.55±2.27 5.28-9.82Heterophils % 49.91±3.50 46.41-53.41Lymphocytes % 44.18±3.36 40.82-47.54Monocytes % 4.40±1.63 2.77-6.033
Page 1: EAAV 2005Abu Dhabi Falcon Hospital,
Page 5 and 6: Table 5: Literature data of hematol
Page 7: 6. CAMPBELL T.W. Avian hematology.

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