Comparative study on the in vitro multiplication potential of Magnolia ...

“FACULTATEA DE HORTICULTURĂ ŞI SILVICULTURĂ”Rooted plantlets had been acclimatized in the greenhouse and transferring to the soil substrate (fig. 8).Fig. 8. Magnolia plants after transfer from greenhouse conditions to soilConclusions1. The results presented in this paper indicatethat the percentage of regenerating, shoot number perexplant, percentage of rooting were affected bygenotype and the interaction of genotype with theculture media. Between Magnolia stellata andMagnolia x soulangiana species tested, the best resultsas well as percent of regeneration, number of shootsper explant and rooting rate were recorded forMagnolia stellata species.2. Explants of apical buds represent optimalsource of inoculums. The period most indicated forsampling and inoculating explants is November-December when vegetative buds are in the dormantstage.3. Research in the evaluation ofmorphogenetic capacity of explants on differentnutrient regeneration formulas and results obtainedhave shown that the best results in terms of percentageof explants started the trend was obtained on theversion supplemented with 0.7 mg/l BAP, 1 mg/lNAA, 0.1 mg/l GA 3 , 5 mg/l ascorbic acid and vitaminsLS.4. Our results demonstrate the clearlyinfluence of citokinine type on the regeneration rate.The best shoot multiplication rate (10.7microshoots/explant in the case of Magnolia stellataspecies and 10 microshoots/explant at Magnolia xsoulangiana) was established with Murashige-Skoogmineral salts, Miller vitamins and 0,5 mg/l BAP.5. It took only 17 days for root initiation ofmagnolia shoots. The best concentration ofsupplemented IBA for root initiation was 4 mg/l.6. The results obtained in our investigationsproved that in vitro culture could be used as anefficient alternative method for multiplication ofMagnolia stellata and Magnolia x soulangiana species.Bibliography1. Biederman I. E. G. - 1987 - Factors affectingestablishment and development of Magnolia hybrids invitro, Acta Horticulturae, 212, s. 625-629.2. Callaway D.J. – 1994 - The world of Magnolias,Timber Press, Portland, 260 pp.3. Kamenicka Aurelia, Lanakova Maria – 2000 -Effect of medium composition and type of vesselclosure on axillary shoot production of magnolia invitro, Acta Physiologiae Plantarum, vol: 22, number: 2,pages: 129-134.4. Kamenicka Aurelia, Takats J. – 1997 - Magnolia, 1:1-6.5. Liu Y.H. – 2004 - Magnolia of China. BeijingScience&Technology Press, 391 pp.6. Merkle S.A. - 1995 - Somatic embryogenesis inMagnoliaceae (Liliodendron and Magnolias). In: BajajY.P.S. (ed.) Somatic embryogenesis and SyntheticSeed I. Springer-Verlag, Berlin, Heidelberg: 388-403.7. Merkle S.A., Watson-Pauley B.A. – 1994 - Ex vitroconversion of pyramid magnolia somatic embryos,HortScience, v. 29(10) p. 1186-1188.8. Tobe J.D. – 1990 - Journal of Magnolia Soc., 26 : 4844