12.07.2015 Views

Palm Oil: A Success Story in Green Technology Innovations

Palm Oil: A Success Story in Green Technology Innovations

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log10 rescaled norm exprRESEARCH IN OIL PALM TISSUE CULTURE• Solid culture system• Liquid culture system• Development of biomarkerExpression Profile of Embryogenic MarkersExplantEgRLK1OPZE5EgRLK1 EgRLK2 EgRLK3EgRLK2 OPZE3CallogenesisOPZE5 OPZE3EgRLK3Embryogenic calliNon-embryogenic calliEgPER1 EgHOX1 (conta<strong>in</strong>s PEMs)Selection of calli for <strong>in</strong>itiation of suspensionOPHb1 EgPK1OPZE3EgRLK3 EgPER1 EgHOX1 Suspensioncultures EgNAC1 OPZE5EgRLK2 EgRLK1 OPHb1Proliferation of embryogenic calli/early stage embryosProgression of embryogenesis suppressed by aux<strong>in</strong>sEgPER1OPZE3EgNAC1Hormone-free mediumEgPER1 Expression profile <strong>in</strong> AM12-14 ProfileOPZE5Rapid progression of embryogenesisPattern formation10OPHb1 Meristem establishmentOPZE5EgPER1Transferred to solid media 1 for embryoid maturation(white & green embryoids)EgNAC1OPZE30.1OPZE3Shoot<strong>in</strong>gEgRLK1EgRLK3OPZE5 0.01EgRLK2T0 T1d T7d T1m T2m T3m T3mA T4m T5m T6m T6mA T9m T9mARoot<strong>in</strong>gstagesPre-nurseryAM12(L) LOW AM13(0) ZERO AM14(H) HIGHCourtesy: DG, MPOB

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