PCR Products - Appleton Woods Ltd

19 PCR PRODUCTSTHERMAL CYCLERSPCR PRODUCTSnewPersonal Thermal Cycler Multigene II TM , LabnetCompact and sleek designReliable performance with heating and coolingbeing accomplished electronically by peltierunitsExcellent uniformity across the block at±0.4°C at 55°CEasy-to-use software allows for successivetime and temperature increments anddecrements, auto-restart after a power failure,end of cycling elongation steps and extendedperiod soaks at 4°CTemperature control range:ambient +4°C to 99 °CHeating rate: up to 2.2°C/secCooling rate: up to 1.9°C/secSupplied with a heated lid for oilfree cyclingA choice of thermal cycler with 25 x 0.2mltube block or 16 x 0.5ml block2 year warrantyCODE DESCRIPTION PRICEMB0500 Multigene II, with block for 25 x 0.2ml tubes 1444.00MB0501 Multigene II, with block for 16 x 0.5ml tubes 1444.00newPersonal Thermal Cycler TC-3000, TechneCompact, low cost - ideal for personal useTwo interchangeable block formats - 25 x0.2ml tubes and 20 x 0.5ml tubesIntuitive programming with ready-to-gotemplatesUnparalleled block uniformity (± 0.1°C at50°C) ensuring optimal reproducibilityRapid heating rate of 3.6°C/secHeated lid (100°C-115°C) sprung to fit 0.2mldomed cap and 0.5ml flat cap tubes, can bedisabled or enabledCan be used stand alone or PC controlled4 year warrantyCODE DESCRIPTION PRICEMB0003 TC-3000 for 25 x 0.2ml microtubes 1900.00MB0004 TC-3000 for 20 x 0.5ml microtubes 1900.00Interchangeable BlocksCODE DESCRIPTION PRICEMB0005 Block for 25 x 0.2ml microtubes 640.00MB0006 Block for 20 x 0.5ml microtubes 640.00Thermal Cycler TC-412, TechneHigh performance, high sample throughputcyclerFunctions include: intuitive, alphanumericprogramming, password protection andcopy/edit functionsFully interchangeable block system allowsblocks to be exchanged in a matter ofseconds, without any toolsDefined pressure, heated lid (100°C - 115°C)to accommodate a variety of consumablesExceptional control, accuracy and temperatureuniformityOptional PC control using Gensoft software -up to 32 cyclers can be connected to a PCand linked togetherTCQ-412 - reduced price package of4 cyclers with all accessories to link to a PC4 year warrantyInterchangeable BlocksCODE DESCRIPTION PRICEMB0151 Block for 60 x 0.5ml microtubes 1185.00MB0152 Block for 96 x 0.2ml microtubes or 96 well plate 1185.00MB0153 Block for 96 x 0.2ml microtubes or 96 well fully skirted plate 1185.00MB0154 Block for flat plate for in-situ 1185.00MB0155 Block for 384 well microplate 1185.00CODE DESCRIPTION PRICEMB0053 TC-412 for 96 x 0.2ml microtubes or 96 well plate 3600.00MB0054 TC-412 for 96 x 0.2ml microtubes or 96 well fully skirted plate 3600.00MB0056 TC-412 for 60 x 0.5ml microtubes 3600.00MB0057 TC-412 for flat plate for in-situ 3600.00MB0055 TC-412 for 384 well microplate 3600.00MB0253 TCQ-412 for 4 x MB0053 14560.00MB0254 TCQ-412 for 4 x MB0054 14560.00MB0255 TCQ-412 for 4 x MB0055 14560.00116Tel 0121 472 7353 E-mail sales@appletonwoods.co.uk Fax 0121 414 1075 Freefax Orderline 0800 387 462

PCR PRODUCTSTHERMAL CYCLERS19Gradient Thermal Cycler TC-512, TechneMost accurately controlled lineargradient due to 4 fold control - eachblock consists of 8 peltier unitscontrolled by 4 independenttemperature thermistors (control sensors)Intuitive programming with ready-to-gotemplates (free updates available)Large touch sensitive screen (115 x90mm)Real time graphical display showingtemperature profile including upper andlower limits of gradientMemory cards provide a flexible andsecure way of storing programmesGradient calculator function displays thetemperature of each column of tubesensuring replication of experimentalconditionsHeating rate of 3°C/sec with < ± 0.4°Cblock uniformity with or withoutgradientNew programmable “defined pressure”flexible heated lid accommodates avariety of consumablesUser-friendly interchangeable blocksystemOther features include PC control optionand decremental/incremental time andtemperature4 year warrantyPCR PRODUCTSBlocksCODE DESCRIPTION PRICEMB0111 Gradient block for 96 x 0.2ml microtubes or 96 well plate 1495.00MB0114 Gradient block for 96 x 0.2ml microtubes or 96 well fully skirted plate 1495.00MB0112 Gradient block for 60 x 0.5ml microtubes 1495.00MB0113 Flat plate block for in-situ (non-gradient) 1495.00MB0115 384 well plate block 1495.00CODE DESCRIPTION PRICEMB0101 TC-512 for 60 x 0.5ml microtubes 4738.00MB0102 TC-512 for 96 x 0.2ml microtubes or 96 well plate 4738.00MB0104 TC-512 for 96 x 0.2ml microtubes or 96 well fully skirted plate 4738.00MB0103 TC-512 for in-situ (non-gradient) 4738.00MB0105 TC-512 for 384 well microplate 4738.00Technical Information for TC-3000 & TC-412MODEL TC-3000 TC-412TEMPERATURE RANGE 4 to 99°C 4 to 99°CMAXIMUM RAMP RATE 3.6°C/sec 2.6°C/secUNIFORMITY AT 50°C ±0.1°C ±0.3°CTEMPERATURE SET POINT PRECISION 0.1°C 0.1°CNUMBER OF PROGRAMMES* 80 80PROGRAMMING 4-line alphanumeric 4-line alphanumericMINIMUM HOLDING TIME 1 sec 1 secMAXIMUM HOLDING TIME 18hrs 18hrsVARIABLE PROGRAMMABLE RAMP RATE 0.1°C/sec steps 0.1°C/sec stepsINCREMENTAL/DECREMENTAL TEMPERATURE yes yesINCREMENTAL/DECREMENTAL SEGMENT HOLD TIME yes yesPAUSE AND STOP FACILITY yes yesEND OF PROGRAMME ALARM (can be disabled) yes yesAUTO RESTART ON POWER FAILURE yes yesRUN END TIME CALCULATION yes yesSERIAL PORT RS232 RS232TEMPERATURE SENSOR thermistor thermistorDIMENSIONS L x W x H 330 x 185 x 190mm 420 x 220 x 260mmWEIGHT 6kg 11kgPOWER CONSUMPTION 230 Watts 620 Watts* Dependent on complexity of the programmesTechnical Information for Multigene IIMULTIGENE IIBLOCK CAPACITY25 x 0.2ml tubes/16 x 0.5ml tubesTEMP. CONTROL RANGE 4°C to 99°CHEATING RATEup to 2.2°C/secCOOLING RATEup to 1.0°C/secTEMP. CONTROL ACCURACY±0.5°CTEMP. UNIFORMITY ±0.4°C AT 55°CMAX. NUMBER OF CYCLES 99MAX. NUMBER OF SEGMENTS 9MAX. HOLDING TIME PER STEP99 minutes 99 secondsMAX. INCUBATION TIME99 hours 99 minutesPROGRAM STORAGE CAPACITY 99DIMENSIONS (W X D X H)21.8 x 28.5 x 17.8cmWEIGHT3.2kgELECTRICAL230V, 50/60HzTechnical Information for TC-512TC-512BLOCK TEMPERATUREGRADIENT RANGE 20 to 70°CMAXIMUM/MINIMUM GRADIENT30°C/1°CNUMBER OF TEMPERATURE PROBES 4BLOCK TEMPERATURE RANGE 4 to 99°CMAXIMUM RAMP RATE*3.0°C/secBLOCK UNIFORMITY WITHOUT A GRADIENT*

19 PCR PRODUCTSCORNING THERMOWELL GOLDPolypropylene (PP) PCR Tubes Thermowell GOLD, CorningPCR PRODUCTSUnique cap design with longer cap flangesensures a secure fit to prevent evaporationand sample lossTubes are available with domed cap forefficient heat transfer or flat cap for easypiercing and markingAvailable in two sizes to ensure maximumversatility for virtually all popular thermalcyclersCertified RNase and DNase free andendotoxin safe8 Well PCR Tube Strips and Caps Thermowell GOLD, CorningCODE ALT. DESCRIPTION INNER PACK PRICECT141 3745 0.2ml flat cap 500 1000 51.00clearCT142 3744 0.2ml flat cap 500 1000 51.00assorted coloursCT143 3747 0.2ml dome cap 500 1000 51.00clearCT144 3746 0.2ml dome cap 500 1000 51.00assorted coloursCT145 3750 0.5ml flat cap 500 1000 39.30clearCT146 3749 0.5ml flat cap 500 1000 39.30assorted coloursPRODUCT GROUP CG118Polypropylene (PP) PCR PlatesThermowell GOLD, CorningAvailable in clear or blackAlso available in 384 well format96 Well PP PCR PlatesUltra-thin wallthickness allows theoptimal rapidthermal transferneeded in Real-TimePCRAvailable in a rangeof skirt options to fita variety of thermalcyclers (seecompatibility guideopposite)CODE ALT. DESCRIPTION INNER CASE PRICECT121 3752 Full skirt, clear 10 50 179.30CT122 3751 Full skirt, black 10 50 179.30CT123 3753 Half skirt, clear 10 50 179.30CT124 3755 Half skirt, black 10 50 197.25CT125 3754 Elevated half skirt, clear 10 50 179.30384 Well PP PCR PlatesPRODUCT GROUP CGCODE ALT. DESCRIPTION INNER CASE PRICECT101 3757 Clear 10 50 266.35CT102 3756 Black 10 50 195.35Manufactured from ultra-thin walledmedical grade polypropyleneDesigned for precise fit in heat blocksensuring optimal thermal transferCap options include domed cap strips, oroptically clear flat cap strips designed forReal-Time PCRTube strips and caps are available in innersof 125, in a case of 1250Certified RNase and DNase free andendotoxin safeCertified RNase and DNase freeand endotoxin safePRODUCT GROUP CGCODE ALT. DESCRIPTION PACK PRICECT131 3741 0.2ml 1 x 8 tube strips 10 x 125 517.10naturalCT132 3740 0.2ml 1 x 8 tube strips 10 x 125 517.10assorted coloursCT133 3743 1 x 8 cap strips, domed 10 x 125 202.50clearCT134 3748 Cap strips, domed 10 x 125 202.50assorted coloursCT135 3742 1 x 8 optically clear flat 10 x 125 202.50cap stripsThermowell GOLD Plate Compatibility GuidePRODUCT GROUP CGThermal Cycler 96 well 96 well 384 wellhalf skirt full skirtApplied BioSystems ® GeneAmp ® 9600 XGeneAmp 9700 X XBiometra ® Uno ® X XUno II ® X XT I Thermocycler ® X X XTgradient ® X XTrobot ® X X XBio-Rad ® iCycler TM X XEppendorf MasterCycler ® X XEricomp SingleBlock ® XTwinBlock ®XDeltacycler I ®XFlexi Gene X XGeniusXThermoHybaid PCR Sprint ® XPCR Express ® X X XMultiBlock System X X XTouchdown ® X X XOmnigene X XOmn-E ® X XMJ Research TM PTC 200DNA Engine X X XPTC 225 DNA Tetrad ® X X XPTC 100 X XMWG Primus 96 ® X XPrimus 384 ®XTaKaRa TP 240 ® XTP 3000 ® X XTechne TC-412 ® X X XTC-512 ® X X XTouchgene X ® X X XTC-3000 X X XSequencersApplied BioSystems ABI PRISM ® 3100 X XABI PRISM 3700 X XABI PRISM 3730 X XAmersham Biosciences MegaBACE TM 500 XMegaBACE 1000 Mark IIXMeagBACE 4000XMJ Research TM BaseStation X XTransgenomic X XRT-PCR Thermal CyclersApplied BioSystems ABI PRISM 7000 X XABI PRISM 7700 X X XABI PRISM 7900 HT X XBio-Rad iCycler ® XStratagene Mx 4000 ® XTel 0121 472 7353 E-mail sales@appletonwoods.co.uk Fax 0121 414 1075 Freefax Orderline 0800 387 462

PCR PRODUCTSCORNING PLATES19PCR PRODUCTSPolypropylene (PP) 96 WellPCR Plates CorningFit all popular cyclersRigid plate design - ideal forrobotic handlingUniform well thickness enablesfast and consistent heat transferCan be cut into 3 x 8 strips100% integrity tested to ensurequality and reliabilityWide skirt enables bar codingCertified RNase and DNase freeRe-usable silicone sealing matsavailable - these are certifiedRNase and DNase free andproduce 100% sealing whenused in conjunction with clampor screw down lidAluminium sealing tape alsoavailable for oil free operationswhich can be pierced for accessto the reaction mixCODE ALT. CODE COLOUR VOLUME PACK PRICETA821 6551 natural 200µl 25 76.90PRODUCT GROUP CGPolycarbonate (PC) 96 WellPCR Plates CorningFit all popular thermal cyclers (see table below)*Thermally stable up to 135°CEach plate designed to precisely fit into specific thermal cycler providingoptimum heat transferCertified RNase and DNase freeCODE ALT.CODE DESCRIPTION VOLUME INNER PACK PRICETA801 6509 96 well Model P plate 200µl 1 25 67.80TA802 6510 96 well Model C plate 200µl 1 25 67.80TA803 6511 96 well Model M plate 200µl 1 25 67.80TA804 6511B 96 well Model M plate 200µl 25 25 65.75TA805 6512 96 well Model T plate 170µl 1 25 65.75TA807 6514 96 well Model H plate 300µl 1 25 65.75TA809 6573 96 well DNA-bind Model M plate 200µl 1 25 182.70TA846 6520 Lid for model C,M,H plates - 1 25 54.10* N.B. Plates must be used in conjunction with sealing tape or mat in cyclers with heated lidSome cyclers require teflon coated aluminium spacer block for oil free operationPRODUCT GROUP CGPC Plate Thermal Cycler CompatibilityPLATEModel PCOMPATIBLE THERMAL CYCLERSGeneAmp PCR System 9600**, Barnstead ThermolyneAmplitron II, Techne Cyclogene, and Gene E with 96 x 0.2ml blockModel C Ericomp SingleBlock, TwinBlock, SingleStar, TwinStar, Delta Cycler I,Delta Cycler II, Stratagene Robocycler Gradient 96Model M MJ Research PTC-100-96V, PTC-200 DNA Engine TM , Biometra Uno ThermoblockerCoy Corporation Temp Cycler II, Corbet Research FTS-960,Hybaid OmniGene with Microblock, Quatro BioSystems T-C-40Model T Techne MW-1, MW-2, Cyclogene, and Gene E with 96 well blockModel H Hybaid OmniGene with Omniblock**Requires the use of a space block and frameCODE ALT.CODE DESCRIPTION INNER CASE PRICEPCR Sealing Tapes and Mats CorningAvailable as cap strips, sealingtape in aluminium orpolyethylene, silicone rubbermats and optical sealing tape96 well silicone rubber septamats offer a unique piercablesurface for easy accessThe ultra-clear surface of the 8well flat cap strips (CT135) areideal for Real-Time PCR,fluorescent and luminescentapplicationsAluminium and clearpolyethylene sealing tapesprovide optimal sealing toprevent evaporation and allowfor oil-free operation when usedwith thermal cyclers with heatedlidsSilicone rubber mats are fullyautoclavable and reusable,providing a cost effectivealternative to other sealingmethodsCT151 3087 96 Well silicone rubber septa mat* 10 50 451.85CC733 6569 Aluminium sealing tape - 384 well plate 100 100 38.65CC734 6570 Aluminium sealing tape - 96 well plate 100 100 28.60CC786 3699 Silicone rubber sealing mat - 384 well plate † 1 25 86.95TA842 6555 Silicone rubber sealing mat - 96 well plate † 1 25 75.30TA844 6524 Polyethylene sealing tape - 96 well plate 100 100 32.45TA843 6556 1 x 8 clear cap strips - PP plate 12 120 115.25TA849 6524AL Aluminium sealing tape 20 100 28.10* ABI Compatible † Autoclavable and re-usable, supplied RNase and DNase freeDUCT GRO PRODUCT GROUP CGGSealing Options for Real-Time PCRCODE ALT.CODE DESCRIPTION INNER CASE PRICECT135 3742 1 x 8 optically clear flat cap strips 125 1250 202.50CC739 6575 Universal optical sealing tape 100 100 158.45PRODUCT GROUP CGFor latest specifications and prices please visit our website www.appletonwoods.co.uk119

19 PCR PRODUCTSPCR TUBESPCR PRODUCTSControl your PCR…To obtain the most accurate and reproducibleresults from your PCR, the reaction componentsmust be controlled.This is important not only in diagnosticprocedures, but increasingly in researchapplications also.The performance of the plastics is critical incontrolling both the temperature of the reactionmix, and the loss of sample through evaporation.PCR Tubes0.2ml Tube with Domed CapThin-walled tube with domed cap with glassgradeoptical area – ideal for Real-Time PCRVery strong tube walls that can withstand thehigh temperatures and pressures of heated lidsSuitable for use in most 0.2ml regular andReal-Time thermal cyclers (for compatibility seepage 123)Also available in a frosted version to enhancesignal-to-noise ratios in Real-Time PCRapplications…Enhance your PCRThis new range of PCR tubes, strips and platesfrom Appleton Woods has many innovativefeatures which will enhance the accuracy andreproducibility of regular and Real-Time PCR.Superior QualityA unique blend of polypropylene whichminimises evaporation and enhances heattransfer, yet is flexible and has excellentoptical clarity.Extremely uniform wall thicknesses give aneven and precise reaction temperaturethroughout the well.CODE DESCRIPTION PACK PRICEBT001 0.2ml tube, domed cap, natural 1000 30.95BT041 0.2ml tube, domed cap, frosted 1000 34.00For evaluation samples please contact theSales Office on 0121 472 73530.2ml Tube with Semi-Domed CapPRODUCT GROUP BTThin-walled tube with extra strong semidomedcapSemi-domed cap has glass-grade optical area– ideal for Real-Time PCRSuitable for use in most 0.2ml regular andReal-Time thermal cyclers (for compatibility seepage 123)Also available in a frosted version to enhancesignal-to-noise ratios in Real-Time PCRapplicationsCODE DESCRIPTION PACK PRICEBT021 0.2ml tube, semi-domed cap, natural 1000 30.95BT061 0.2ml tube, semi-domed cap, frosted 1000 34.00PRODUCT GROUP BTNon-uniform tubeAppleton Woods tubeFor cycler compatibility see page 123Enhanced tube geometries for optimal fitin the blocks of leading thermal cyclers.0.2ml Tube with Flat CapThin-walled tube with flat cap withglass-grade optical area – ideal forReal-Time PCRSuitable for use in most 0.2ml regularand Real-Time thermal cyclers (forcompatibility see page 123)Poorly fitting tubeAppleton Woods tubeCODE DESCRIPTION PACK PRICEBT101 0.2ml tube, flat cap, natural 1000 30.95PRODUCT GROUP BTInnovative design of the tube caps givingleak free sealing, yet can be repeatedlyclosed and opened with ease.See also:PCR racks, page 149PCR tube labels, page 90120Tel 0121 472 7353 E-mail sales@appletonwoods.co.uk Fax 0121 414 1075 Freefax Orderline 0800 387 462

PCR Tube Strips0.2ml 8-Well Tube StripHigh profile tube strip that can beused in virtually any 0.2ml regularor Real-Time thermal cycler (forcompatibility see page 123)Use with BS291 flat cap strips orBS261 semi-domed cap strips forsealing (see page 123)CODE DESCRIPTION PACK PRICEBS001 0.2ml high profile, 8-tube strip, natural 120 46.900.2ml Low Profile, 8-Well Tube StripLow profile tube strip that can beused in most 0.2ml regular orReal-Time thermal cyclers withheight adjustable lids (forcompatibility see page 123)Use with BS291 flat cap strips orBS261 semi-domed cap strips forsealing (see page 123)PRODUCT GROUP BTCODE DESCRIPTION PACK PRICEBS131 0.2ml low profile, 8-tube strip, natural 120 55.900.2ml 8-Well Tube Strip with Individually AttachedOptical Domed CapsHigh profile tube strip withindividually attached capsavoiding cross-contaminationAlso available in a frosted versionto enhance signal-to-noise ratiosin Real-Time PCR applicationsCODE DESCRIPTION PACK PRICEBS151 0.2ml 8-tube strip with domed caps, natural 120 59.60BS171 0.2ml 8-tube strip with domed caps, frosted 120 65.600.2ml 8-Well Tube Strip with Individually AttachedOptical Flat CapsHigh profile tube strip withindividually attached capsavoiding cross-contaminationCaps are flat and optically clearPRODUCT GROUP BTPRODUCT GROUP BTCODE DESCRIPTION PACK PRICEBS191 0.2ml 8-tube strip with flat caps, natural 120 59.60PRODUCT GROUP BTPCR PRODUCTSPCR TUBE STRIPSWhy use Appleton WoodsPCR Plastics?Raw MaterialA unique blend of polypropylene designedspecifically for PCRMedical grade – no metals or other contaminantsreleased into the reaction mixOptimised blend:– Low DNA binding – Flexibility – Very low gaspermeability – Anti-static – Good thermalconductivity – High optical clarityImproved manufacturing – no requirement to usesofteners, coatings or mould release agentsTube DesignThin walls wheretube contacts thethermal blockThicker walls at thetop of the tube forstrength and tominimise evaporation High Profile Low ProfileExtremely uniform wall thicknessTube geometry for optimal block fitInnovative cap design gives leak free sealing, yetcan be repeatedly closed and opened with easeLow profile versions allow less reaction mix to gointo the gas phase leading to less concentration ofthe sample, less change in the reaction conditionsand, therefore, more reproducible resultsManufacturing QualityManufactured using high quality finely-tunedinjection moulding machines and moulds, withoutthe need for mould releasing agentsExtremely tight control of all manufacturingconditionsLow number of cavities per mouldManufactured under cleanroom conditions to ensurethe absence of DNA, RNA, DNase, RNase, pyrogensand ATP.Real-Time ApplicationsTube and strip caps are optically clear andallow fluorescent signals to pass with minimal lossof signal intensityOpti-seal disposable film is available forsealing platesWhite plates increase signal/noise ratios, but restrictvisibility of well contentsFrosted versions of tubes and plates enablethe contents of the tube to be seen, but givesignal/noise ratios comparable to solid white platesComparison ofReal-Time signalswhen usingAppleton Woodsblack, white, frostedor natural strips19PCR PRODUCTSFor latest specifications and prices please visit our website www.appletonwoods.co.uk121

19 PCR PRODUCTSPCR PLATESPCR PlatesPCR PRODUCTS96 Well Plate, High Profile, Non-SkirtedNon-skirted, thin wall plate that can beeasily cut into 16, 24, 32 or 48 wellpieces along marker linesMoulded-in alpha-numeric identificationCan be sealed with optical, flat 8-capstrips (BS291), optical, semi-domed 8-cap strips (BS261), Opti-Seal sealing film(BP231) or aluminium seal (BP232)CODE DESCRIPTION PACK PRICEBP001 96 x 0.2ml high profile, non-skirted, natural 25 52.75BP009 96 x 0.2ml high profile, non-skirted, white 25 58.00PRODUCT GROUP BT96 Well Plate, Low Profile, Non-Skirted, FrostedNon-skirted, thin wall plate that canbe easily cut into 16, 24, 32 or 48well pieces along marker linesAll wells are frosted to improveReal-Time PCR signal-noise ratiosMoulded-in alpha-numeric identificationCan be sealed* with optical, flat 8-capstrips (BS291), optical, semi-domed 8-cap strips (BS261), Opti-Seal sealing film(BP231) or aluminium seal (BP232)CODE DESCRIPTION PACK PRICEBP021 96 x 0.2ml low profile, non-skirted, frosted, natural 25 58.00BP029 96 x 0.2ml low profile, non-skirted, frosted, white 25 58.00PRODUCT GROUP BT96 Well Plate, High Profile, Sub-Skirted,Frosted – ABI CompatibleHigh profile, rigid plate with thin wallsand semi-skirted – ideal for robotichandlingThe working volume for this plate istypically between 5µl and 25µlMoulded-in alpha-numeric identificationCan be sealed* with optical,flat 8-cap strips (BS291),optical, semi-domed 8-cap strips(BS261), Opti-Seal sealing film (BP231)or aluminium seal (BP232)CODE DESCRIPTION PACK PRICEBP101 96 x 0.2ml high profile, sub-skirted, frosted, natural 25 65.30BP109 96 x 0.2ml high profile, sub-skirted, frosted, white 25 65.30PRODUCT GROUP BT96 Well Plate, Low Profile, Sub-Skirted,Frosted – ABI CompatibleLow profile, rigid plate with thin wallsand elevated skirt – ideal for robotichandlingThe working volume for this plate istypically between 5µl and 25µlDesigned for use with ABI9800 but canbe used with most regular and Real-Time cyclersAll wells are frosted to improve Real-Time PCR signal-noise ratiosMoulded-in alpha-numeric identificationCan be sealed* with optical,flat 8-cap strips (BS291),optical, semi-domed 8-cap strips(BS261), Opti-Seal sealing film (BP231)or aluminium seal (BP232)*WARNINGTo ensure tubes and plates remain sealed a compressionmat may be required (see compatibility on page 123)CODE DESCRIPTION PACK PRICEBP181 96 x 0.2ml low profile, sub-skirted, frosted, natural 25 58.00BP189 96 x 0.2ml low profile, sub-skirted, frosted, white 25 58.00PRODUCT GROUP BTFor cycler compatibility see page 123122Tel 0121 472 7353 E-mail sales@appletonwoods.co.uk Fax 0121 414 1075 Freefax Orderline 0800 387 462

PCR Tube Cap StripsThin Wall, Semi-Domed 8-Cap StripFrosted writing area atboth ends of stripSemi-domed 8-cap stripwith glass-grade opticalarea – Ideal for Real-Time PCRFor closure of 0.2ml 8-tube strips and 96 well platesCODE DESCRIPTION PACK PRICEBS261 Semi-domed 8-cap strip, natural 120 18.65PRODUCT GROUP BTPCR Plate Sealing FilmOpti-Seal Optical, Self-Adhesive Sealing FilmDisposable adhesive sealing film for 96and 384 well platesNon-pierceable and can be easilyremoved after the PCR reactionIdeal for Real-Time PCRThin Wall, Flat 8-Cap StripFrosted writing area atone end of stripFlat 8-cap strip with glass-gradeoptical area – Ideal for Real-Time PCRFor closure of 0.2ml 8-tube stripsand 96 well platesPCR PRODUCTSCAP STRIPS – SEALING FILMCODE DESCRIPTION PACK PRICEBS291 Flat 8-cap strip, natural 120 22.55Alu-Seal Aluminium, Self-Adhesive Sealing FilmDisposable adhesive sealing film for96 and 384 well platesEasily removed and can bepiercedSuitable for a wide range ofapplications including PCR,incubation and freezer storagePRODUCT GROUP BT19PCR PRODUCTSCODE DESCRIPTION PACK PRICEBP231 Opti-Seal 100 83.00CODE DESCRIPTION PACK PRICEBP232 Alu-Seal 100 83.00For further compatibility information (including DNA sequencers) visit our website www.appletonwoods.co.uk/compatibilityPCR Plastics PCR Tubes PCR Strips PCR PlatesCompatibility ChartBT001BT021BT101BS001BS131BS151BS191BP001BP021BP101BP181Manufacturer Model WellsABI GeneAmp® PCR System 2400 24 ̌ ̌ ABI GeneAmp® PCR System 9600 96 ̌ ̌ ̌ ABI 2700 Thermal Cycler 96 ̌ ̌ ̌ ABI 2720 Thermal Cycler 96 ̌ ̌ ̌ ABI GeneAmp® PCR System 9700 60 ABI GeneAmp® PCR System 9700 96 ̌ ̌ ABI GeneAmp® PCR System 9700 384 ABI 9800 Fast Thermal Cycler 96 fast ̌ ̌Biometra Uno 24/48/96 ̌ Biometra Uno II 24/48/96/384 ̌ Biometra Tpersonal 20/48 Biometra T1 (plus) 48/96/384 ̌ Biometra TGradiant 48/96/384 ̌ Biometra T3/T3000 20/48 Biometra TProfessional 96/384 ̌ Biometra TRobot 96/384 ̌ Bio-Rad Gene Cycler 24 Bio-Rad iCycler® 48/96/384 Bio-Rad MyCycler 96 Corbett Research Palm-Cycler 60/96/384 Eppendorf Mastercycler® 77 Eppendorf Mastercycler® 96 Eppendorf Mastercycler® 384 Eppendorf Mastercycler® gradient 77 Eppendorf Mastercycler® gradient 96 Eppendorf Mastercycler® gradient 384 Eppendorf Mastercycler® personal 16 Eppendorf Mastercycler® personal 25 Eppendorf Mastercycler® ep gradient 96 Eppendorf Mastercycler® ep gradient S 96 Eppendorf Mastercycler® ep 384 384 MJ Research/Bio-Rad PTC-150 16/25 MJ Research/Bio-Rad Mini 48 MJ Research/Bio-Rad PTC-100 60/96 MJ Research/Bio-Rad PTC-200 30/48/96/384 MJ Research/Bio-Rad PTC-221 30/48/96/384 MJ Research/Bio-Rad PTC-220 30/48/96/384 MJ Research/Bio-Rad PTC-225 30/48/96/384 PCR Plastics PCR Tubes PCR Strips PCR PlatesCompatibility Chart (continued)Manufacturer Model WellsTechne Genius 40/96/384 Techne TouchGene 40/96 Techne FlexiGene/FlexiQuad 96/384 Techne Progene 20/25 Techne TechGene 25/96 Techne Gene-E 96 Techne TC-312 20/25 Techne TC-412 96 Techne TC-512 96/384 ThermoHybaid PxE 96 ThermoHybaid OmniGene 96 ThermoHybaid PCR Express 48/96/384 ThermoHybaid Omn-E 96 ThermoHybaid TouchDown 48/96 ThermoHybaid Px2 96/384 ThermoHybaid PCR sprint 24 ThermoHybaid MBS 96/384 ThermoHybaid MBSR 96/384 Real-Time PCR PCR Tubes PCR Strips PCR PlatesCompatibility ChartManufacturer Model WellsABI GeneAmp® SDS 5700 96 ̌ ABI Prism® SDS 7000 96 ̌ ABI Prism® SDS 7300 96 ̌ ABI Prism® SDS 7500 96 ̌ ABI Prism® SDS 7500 96 fast ABI Prism® SDS 7700 96 ̌ ABI Prism® SDS 7900 96 ABI Prism® SDS 7900 384 ABI Prism® SDS 7900 96 fast Bio-Rad iCycler iQ® 96 Bio-Rad iQ5 96 Bio-Rad MyiQ 96 Eppendorf MasterCycler® ep realplex 96 MJ Research/Bio-Rad Opticon® 96 MJ Research/Bio-Rad Opticon®2 96 MJ Research/Bio-Rad MiniOpticon® 48 MJ Research/Bio-Rad Chromo4 96 ̌ Recommended for this cycler Fits this cycler Minimum of 20 tubes per block Can be cut to fit this cycler Use of compression mat recommended Does not fit this cyclerFor latest specifications and prices please visit our website www.appletonwoods.co.ukBT001BT021BT101BS001BS131BS151BS191BP001BP021BP101BP181BT001BT021BT101BS001BS131BS151BS191BP001BP021BP101BP181123

19 PCR PRODUCTSPCR PRODUCTSPCR ® TubesA B C D0.2ml to 0.5ml TubeAdaptorsHigh quality RNase and DNase freepolypropylene tubes, non sterileThin walls give rapid cycling times leading tomore effective PCRDimensional uniformity ensures optimumblock contactA 0.2ml Flat Cap TubeCODE COLOUR PACK PRICETA511 natural 1000 37.00TA512 blue 1000 39.20TA513 green 1000 39.20TA514 red 1000 39.20C0.5ml Dome Cap TubePRODUCT GROUP PCRCODE COLOUR PACK PRICETA521 natural 1000 23.35PRODUCT GROUP PCRBFit all popular thermal cyclersEasy to open/close, leak-free sealsDomed cap tubes facilitate efficient heattransfer from cycler’s lidFlat cap frosted for easy marking and can bepierced0.2ml Dome Cap TubeCODE COLOUR PACK PRICETA501 natural 1000 37.00TA502 blue 1000 39.20TA503 green 1000 39.20TA504 red 1000 39.20D 0.5ml Flat Cap TubePRODUCT GROUP PCRCODE COLOUR PACK PRICETA531 natural 1000 23.35PRODUCT GROUP PCRGraduated for volume estimationProtects fragile PCR tubes duringcentrifugationWorks in most 0.5ml racks and centrifugesCODE DESCRIPTION PACK PRICETA519 0.2ml - 0.5ml tube adaptors 100 13.35PRODUCT GROUP PCR8 Well PCR Tube Strips and CapsAvailable as:– Separate strips and caps (A & B)– Strips with end attached cap strips (C)– Strips with individual attached caps (D)Flat cap strips are optically clear and piercableCompatible with multichannel pipettesTA571 strips can be broken to reduce lengthBACODE DESCRIPTION PACK PRICETA541 0.2ml 1x8 tube strips A 125 58.35naturalTA561 1x8 end attached cap C 125 67.90strips, naturalTA571 1x8 individual attached D 125 67.90cap strips, naturalTA551 1x8 separate cap strips B 125 13.50domedTA552 1x8 separate cap strips 125 14.05domed, blueTA581 1x8 separate cap strips 125 12.20flatPRODUCT GROUP PCRCD124Tel 0121 472 7353 E-mail sales@appletonwoods.co.uk Fax 0121 414 1075 Freefax Orderline 0800 387 462

UV PCR SterilisationCabinets & WorkstationsUVPControls PCR contamination with high intensity UV tubesAvailable in two sizes - Cabinet and Workstation, with thecabinet having a smaller footprintCoated stainless steel design provides continuousantimicrobial protection30 minute mechanical timer to control theUV decontaminationSafety shut-off switch when door is openedAcrylic enclosure protects against UV and ß radiationHinged door flips up for easy access to the work areaBuilt-in power outlets allow operation of equipment for use inPCR applicationsPCR PRODUCTSPCR CABINETSnew19PCR PRODUCTS3 models available: Standard UV PCR, standard UV PCR withUV/air recirculation and UV/HEPA PCR with UV/airrecirculationThe UV/HEPA systems feature four-stage filters, three UVsources and antimicrobial stainless steel coating for maximumcontainment control12 month warrantyMB0081CODE DESCRIPTION PRICEMB0080 UV PCR Sterilisation Cabinet 960.00MB0081 UV PCR Sterilisation Workstation 1040.00MB0083 UV PCR Sterilisation Cabinet with UV/air recirculation 1180.00MB0084 UV PCR Sterilisation Workstation with UV/air recirculation 1280.00MB0085 UV/HEPA PCR Sterilisation Cabinet with UV/air recirculation 1830.00MB0086 UV/HEPA PCR Sterilisation Workstation with UV/air recirculation 1930.00Technical InformationPlease note: Units are supplied flat packed and require self-assemblyMODEL MB0080 MB0081 MB0083 MB0084 MB0085 MB0086SIZE Cabinet Workstation Cabinet Workstation Cabinet WorkstationEXT. DIMENSIONS (H x W x D) 729 x 544 x 610mm 729 x 737 x 610mm 729 x 544 x 610mm 729 x 737 x 610mm 826 x 544 x 610mm 826 x 737 x 610mmINT. DIMENSIONS (W x D) 500 x 544mm 709 x 544mm 500 x 544mm 706 x 544mm 500 x 544mm 706 x 544mmWHITE LIGHT 8 Watts 8 Watts 8 Watts 8 Watts 8 Watts 8 WattsUV LIGHT 8 Watts 8 Watts 8 Watts 8 Watts 8 Watts 8 WattsUV/ AIR RECIRCULATION No No Yes Yes Yes Yes4 STAGE HEPA FILTER No No No No Yes YesINTERNAL POWER OUTLETS 2 4 2 4 2 4– Equipping a new laboratory?– Receiving funding for newequipment?…then join our New Lab Scheme and you will not onlybenefit from great savings but we will also provide help andsupport; from site surveys to co-ordination of deliveries.SEE CONTENTS PAGE FOR MORE DETAILSFor latest specifications and prices please visit our website www.appletonwoods.co.uk125