In Vitro Callus Induction from Stem Explants of Cissus ...

In Vitro …Kalpana Teware et al.Table: 1- Responses of callusing of Cissus quadrangularis L. in different month of year.S.NO. Months Callus induction1 Jan – Feb ++2 Mar-April +3 May-June ++++4 July- Aug ++++++5 Sep_Oct. +6 Nov- Dec. +Table: 2- Effect of various level and combinations of PGR’s for callusing of Cissusquadrangularis L.S.No. MS-Medium 2,4,D NAA Type of the callus Responses in%1 CM1 0.5 - No callus induction 0%2 CM2 1.0 - Dark brown friable callus 25%3 CM3 1.5. - Brown friable callus 35%4 CM4 2.0 - Light brown friable callus 50%5 CM5 2.5 - Light brown friable callus 65%6 CM6 3.0 - Slimy light brown friable callus 85%7 CM7 3.5. - Brown non friable callus 90%8 CM8 4.0 - Brown non friable callus 92%9 CM9 - 0.5 Light brown non friable callus 35%10 CM10 - 1.0 Light green non friable callus 40%11 CM11 1.5 Light brown whitish callus 50%12 CM12 2.0 Light green whitish callus 50%13 CM13 2.5 Light brown whitish callus 50%14 CM14 3.0 Light brown white non friable 65%callus15 CM15 3.5 Light brown white callus 70%16 CM16 4.0 Light brown non friable callus 75%1270 J. Chem. Bio. Phy. Sci. Sec. A, 2013, Vol.3, No.2, 1268-1272.

In Vitro …Kalpana Teware et al.Fig. 1: Responses of Callusing In Different Month of The Year (2009-10) for Cissusquadrangularis L. HadjodFig. 2: Responses of Callusing with Different PGR’s with MS Medium inCissus quadrangularis L. (Hadjod)Fig. 3: Callus responses of Cissus quadrangularis L. with MSMedium of different composition.1271 J. Chem. Bio. Phy. Sci. Sec. A, 2013, Vol.3, No.2, 1268-1272.

In Vitro …Kalpana Teware et al.REFERENCES1. A.W.Panthong, D. Supraditaporn,T. Kanjanapothi, Taesotikul and V. Reutrakul. An analgesic,anti-inflammatory and venotonic effect of Cissus quadrangular is Linn. J. Ethnopharmacol,2007, 110: 264-270.2. S.P.Sen, Studies on the active constituents of Cissus quadrangularis L. Wall. Curr. Sci,1966, 35:317.3. K.K.Bhutani,R. Kapoor, C.K. Atal, Two unsymmetrical Tetracyclic triterpenoids from Cissusquadrangularis L .Phytochemistry,1984,23 (2),407.4. Anuj K.Srivastava1, Priyanka Shrivastava, B.R. Behera, A.K. Shrivastava,; Pharmacognostical& Phyto-Chemical Investigation of Cissus quadrangularis Linn. Stem, 2011, 3, 020, 974–9446.5. T.Murashige, and I. A. Skoog, Physiole plant, 1962, 16:473-497.6. Mahendra Peenu, Singh Pratibha and Khan Shagufta.In vitr regeneration studies ofWithania sominifera (L.) Dunal. Indian J. of Applied and pure Biology, 2004, 19(3):359-362.7. Khan, N. Alam, M.S. Nath; In Vitro regeneration of Garlic trough callus culture. J. ofBiological sci. U.K. 2004,8. Datta Anirudha , Narula Alka,K.C. Banali, P.S. Shrivastava, Enhance alkaloid production incallus culture of liotropium indicu L. and regeneration of plantlets J. of Plant BiotechnologBiochemistry,2003,12: 139-142.9. Geta Rani , G.S.Virk, and Nagpal Avinash,; callus induction and plantlets regeneration onWithenia sominifera L. in vitro cellular anddevelopmental biology plant,2003, 39(5) : 468-474.Corresponding author: Teware Kalpana; Department of Bioscience SAM GirlsP.G. College, Bhopal(M.P.) India1272 J. Chem. Bio. Phy. Sci. Sec. A, 2013, Vol.3, No.2, 1268-1272.