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31. List environmental factors which can influence generation timeMICROBIOLOGYBIOS-246032. Discuss some factors that decrease the accuracy of counting bacteria33. List procedures employed to obtain a lyophilized culture34. Explain why serial dilutions are required when making most bacterialcounts35. Define microbistasisD. Unit 4At the completion of this unit you should be able to:1. Compare the structural diversity of living organisms with theirbiochemical diversity2. Distinguish between oxidation and reduction3. Provide the functions of dehydrogenase, NAD and FAD in cellrespiration4. Discuss enthalpy, entropy and free energy5. Relate the second law of thermodynamics to the functioning of a livingorganisms6. Explain the concept of free energy and the manner in which energyreleased in exergonic reactions is used to drive endergonic reactions7. Distinguish between catabolic and anabolic reactions8. Expand on the statement that most any material on earth can nourishone microbe or another9. Discuss the events which occur during glycolysis, citric acid cycle andthe electron transport system10. Compare the energy field from aerobic and anaerobic cell respiration11. Explain the significance of the cytochromes and the importance of theoxidase test12. Provide examples of electron acceptors in anaerobic cell respiration13. Provide examples of substrates utilized by the chemolithotrophs14. Explain the importance of amphibolic reactions11