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Sensitivity can be calculated using the following formula under the guidelines listed in theNational Committee for Clinical Laboratory Standards (NCCLS) Evaluation Protocols (6) .2SD of the Zero Standard0.21 ng/mLSensitivity (ng/mL)(Optical Density of 0ng/mLOptical Density of 0.21ng/mL)< Precision and reproducibility >Intra-assay variation (mean±SD, n=10) Inter-assay variation (mean±SD, n=9) Measured (ng/mL) %CV Measured (ng/mL) %CVQC sample 1 0.767±0.036 4.7 0.767±0.063 8.2QC sample 2 2.802±0.105 3.7 2.655±0.205 7.7QC sample 3 7.837±0.197 2.5 6.951±0.683 9.8< Analytical recovery >Mouse serum Corticosterone Observed Expected Recoveryadded (ng/mL) (ng/mL) (ng/mL) (%)No. 1 0 5.9331.52 7.728 7.453 103.74.55 10.202 10.483 97.313.64 18.696 19.573 95.5No. 2 0 4.6601.52 5.854 6.180 94.74.55 8.464 9.210 91.913.64 15.779 18.300 86.2No.3 0 2.6291.52 3.943 4.149 95.04.55 6.530 7.179 91.013.64 14.148 16.269 87.0Mouse plasma Corticosterone Observed Expected Recoveryadded (ng/mL) (ng/mL) (ng/mL) (%)No.1 1.52 4.257 4.358 97.74.55 6.577 7.388 89.013.64 14.120 16.478 85.7No.2 0 2.8431.52 4.215 4.363 96.64.55 6.569 7.393 88.97