FRAMINGHAM HEART STUDY CODING MANUAL

FRAMINGHAM HEART STUDY CODING MANUALMultiplexed Immunoassay Panel 1 (SABRe project)COHORT: Offspring, Generation 3TIME INTERVAL: Offspring Cohort Exam 7, Generation 3 Cohort Exam 1DATASET NAME:l_mpimn01_2005_m_0692s# OBSERVATIONS: 7357Variables that define a unique record: SHAREID.This is the first in a series of immunoassay panels done on the same group of participants (Offspringcohort exam 7 and Gen 3 cohort exam 1). Please combine this dataset with the following datasets to geta full list of all the immunoassays done: l_mpimn02_2005_m_0693 and l_mpimn03_2005_m_0694.Upper CV limits were applied separately to each assay and each cohort group to remove extreme CVoutliers. The immunoassay value and the CV for all records above this upper CV limit was set tomissing.The value ranges, number of observations and cohorts stated in this manual are based on the original dataset.In some cases, observations may be deleted due to participant consent form restrictions or cohort participation.If observations have been deleted from this dataset, the value ranges, number of observations or cohorts maydiffer from those stated in this manual.FHS Coding Manual for l_mpimn01_2005_m_0692sRevised: 8/3/2012 8:54:23 PMPage 1 of 8

Text16: IDTYPEVariable name:Description:IDTYPEFramingham Heart Study cohort identifierCode or Value Description Count1 Offspring Cohort3 Generation 3 Cohort32944063Text16: IDVariable name:Description:IDFramingham Heart Study identifier within a cohortNote:IDTYPE and ID taken together form the study participant ID NUMBER.This Framingham dataset has been modified. ID has been deleted. The SHAREID replaces IDTYPE andID as a unique study participant identifier.Text16: SHAREIDVariable name:Description:SHAREIDUnique study participant identification numberText16: RUN_DATEVariable name:Description:Units:RUN_DATEAssay Run Datenumber of days since exam 1Code or Value Description Count2196 — 14566 Range of values7357FHS Coding Manual for l_mpimn01_2005_m_0692sRevised: 8/3/2012 8:54:23 PMPage 2 of 8

Text16: PLATE_IDVariable name:Description:PLATE_IDID number for plateCode or Value Description CountBlankField not filled in0Non-blankField filled in7357Note:Character variableText16: CLUSTERINVariable name:Description:Units:CLUSTERINClusterin(sample type: plasma)pg/mLCode or Value Description Count15000 — 206000000 Range of values7324. Unknown33Note:The minimum detectable limit for CLUSTERIN is 15000 pg/mL. Values below 15000 are set to 15000.Use CLUSTERIN_I to see what records are below the detectable limit.CLUSTERIN values were set to unknown if CLUSTERIN_CV was greater than or equal to 32.9 forOffspring (IDTYPE = 1), or 25.7 for Gen 3 (IDTYPE = 3).Text16: CLUSTERIN_CVVariable name:Description:CLUSTERIN_CVCV for ClusterinCode or Value Description Count0 — 25.3 Range of values7318. Unknown39Note:CLUSTERIN_CV values were set to unknown if CLUSTERIN_CV was greater than or equal to 32.9 forOffspring (IDTYPE = 1), or 25.7 for Gen 3 (IDTYPE = 3).FHS Coding Manual for l_mpimn01_2005_m_0692sRevised: 8/3/2012 8:54:23 PMPage 3 of 8

Text16: CLUSTERIN_IVariable name:Description:CLUSTERIN_IIndicator variable for ClusterinCode or Value Description Count1 Below measurable range62 Within measurable range3 Above measurable range4 Set to missing because CV was above allowable limit7318033Text16: APO_A1Variable name:Description:Units:APO_A1Apolipoprotein-A1(sample type: plasma)pg/mLCode or Value Description Count343000 — 5190000000 Range of values7345. Unknown12Note:The minimum detectable limit for APO_A1 is 343000 pg/mL. Values below 343000 are set to 343000.The maximum detectable limit for APO_A1 is 5190000000 pg/mL. Values above 5190000000 are setto 5190000000. Use APO_A1_I to see what records are below the detectable limit.APO_A1 values were set to unknown if APO_A1_CV was greater than or equal to 73.3 for Offspring(IDTYPE = 1), or 80.2 for Gen 3 (IDTYPE = 3).Text16: APO_A1_CVVariable name:Description:APO_A1_CVCV for Apolipoprotein-A1Code or Value Description Count0 — 77.1 Range of values7335. Unknown22Note:APO_A1_CV values were set to unknown if APO_A1_CV was greater than or equal to 73.3 forOffspring (IDTYPE = 1), or 80.2 for Gen 3 (IDTYPE = 3).FHS Coding Manual for l_mpimn01_2005_m_0692sRevised: 8/3/2012 8:54:23 PMPage 4 of 8

Text16: APO_A1_IVariable name:Description:APO_A1_IIndicator variable for Apolipoprotein A1Code or Value Description Count1 Below measurable range62 Within measurable range3 Above measurable range4 Set to missing because CV was above allowable limit7335412Text16: APO_B100Variable name:Description:Units:APO_B100Apolipoprotein-B100(sample type: plasma)pg/mLCode or Value Description Count2570000 — 545000000 Range of values7356. Unknown1Note:The minimum detectable limit for APO_B100 is 2570000 pg/mL. Values below 2570000 is set to2570000. Use APO_B100_I to see what records are below the detectable limit.APO_B100 values were set to unknown if APO_B100_CV was greater than or equal to 45.4 for Gen 3(IDTYPE = 3).Text16: APO_B100_CVVariable name:Description:APO_B100_CVCV for Apolipoprotein-B100Code or Value Description Count0 — 57.6 Range of values1580. Unknown5777Note:APO_B100_CV values were set to unknown if APO_B100_CV was greater than or equal to 45.4 for Gen3 (IDTYPE = 3).FHS Coding Manual for l_mpimn01_2005_m_0692sRevised: 8/3/2012 8:54:23 PMPage 5 of 8

Text16: APO_B100_IVariable name:Description:APO_B100_IIndicator variable for Apolipoprotein-B100Code or Value Description Count1 Below measurable range57762 Within measurable range3 Above measurable range4 Set to missing because CV was above allowable limit158001Text16: LPAVariable name:Description:Units:LPALipoprotein(a)(sample type: plasma)pg/mLCode or Value Description Count3200000 — 3840000000 Range of values7303. Unknown54Note:The minimum detectable limit for LPA is 3200000 pg/mL. Values below 3200000 are set to 3200000.Use LPA_I to see what records are below the detectable limit.LPA values were set to unknown if LPA_CV was greater than or equal to 34.0 for Offspring (IDTYPE = 1)or 34.9 for Gen 3 (IDTYPE = 3).Text16: LPA_CVVariable name:Description:LPA_CVCV for Lipoprotein(a)Code or Value Description Count0 — 34.6 Range of values6975. Unknown382Note:LPA_CV values were set to unknown if LPA_CV was greater than or equal to 34.0 for Offspring (IDTYPE= 1) or 34.9 for Gen 3 (IDTYPE = 3).FHS Coding Manual for l_mpimn01_2005_m_0692sRevised: 8/3/2012 8:54:23 PMPage 6 of 8

Text16: LPA_IVariable name:Description:LPA_IIndicator variable for Lipoprotein(a)Code or Value Description Count1 Below measurable range3282 Within measurable range3 Above measurable range4 Set to missing because CV was above allowable limit6975054Text16: CRPVariable name:Description:Units:CRPC-Reactive Protein(sample type: plasma)pg/mLCode or Value Description Count2000 — 34700000 Range of values7322. Unknown35Note:The minimum detectable limit for CRP is 2000 pg/mL. Values below 2000 are set to 2000. Themaximum detectable limit for CRP is 34700000 pg/mL. Value above 34700000 are set to 34700000.Use CRP_I to see what records are below the detectable limit.CRP values were set to unknown if CRP_CV was greater than or equal to 40.8 for Offspring (IDTYPE =1) or 36.0 for Gen 3 (IDTYPE = 3).Text16: CRP_CVVariable name:Description:CRP_CVCV for C-Reactive ProteinCode or Value Description Count0 — 36.6 Range of values7303. Unknown54Note:CRP_CV values were set to unknown if CRP_CV was greater than or equal to 40.8 for Offspring (IDTYPE= 1) or 36.0 for Gen 3 (IDTYPE = 3).FHS Coding Manual for l_mpimn01_2005_m_0692sRevised: 8/3/2012 8:54:23 PMPage 7 of 8

Text16: CRP_IVariable name:Description:CRP_IIndicator variable for C-Reactive ProteinCode or Value Description Count1 Below measurable range92 Within measurable range3 Above measurable range4 Set to missing because CV was above allowable limit73031035FHS Coding Manual for l_mpimn01_2005_m_0692sRevised: 8/3/2012 8:54:23 PMPage 8 of 8