Ultra BCA Protein Assay Kit, Industry's First with “Pre-coated” - Alpha ...

Ultra BCA Protein Assay Kit, Industry’s First with “Pre-coated” Standards1.61.41.210.80.60.40.200.00.20.40.81.53C o n c e n t r a t i o n ( u g )612BSABGGUltra BCA Protein Assay-Highlights• Convenient, stable, ready to use pre-coated, error free BSAstandards (12, 6, 3, 1.5, 0.75, 0.375, 0.1875, 0 g). No dilutionof standards saves time and prevents errors.• Bovine Gamma Globulin liquid standard at 1 mg/ml for usagewith protein samples containing Ig’s (1.0 ml x 1vial).• Range ~0.2 - 12 g; 150 l samples; Sensitivity ~0.2 g.• 60-minute incubation at 37 o C. Read A562nm (540-590nm); timecan be adjusted from 30-120 mins at room temperature.For in vitro research use only.Kit Contents1. Ultra BCA Protein Reagent A: 20.0 ml2. Ultra BCA Protein Reagent B: 0.5 ml3. Standards: BSA Pre-coated strip, 12 strips as 2 x 6 sets (12, 6, 3, 1.5, 0.75, 0.375, 0.1875, 0 g).4. BGG (1 vial of 1 mg/ml)5. Pre-optimized strip plates for all BCA tests assure 100% compatibility. Save time and hassle to buy separate items.Reagent PreparationUltra BCA Protein assay requires 150 l of working reagent per well. Determine the total samples and 8 standards includingblank. Mix reagent A and B in 50:1 ratio (10 ml of A and 0.2 ml of B). Prepare just before the assay. Working reagent can bestored at room temp for several hours.Assay Procedure: Allow all reagents to reach room temperature. Arrange and label required number of strips.Prepare the BCA working reagents by mixing Reagents A and B (50:1). Dilute samples if necessary.Step 1. Use “pre-coated standard strip plate” and protein samples in duplicate into a fresh ELISA strip plate (wells).Dispense 150 l of BCA working reagent into all wells. Mix gently, cover the plate and incubate at 37 o C for 60 min. It isalso possible to incubate at room temp 25-28 o C (2 hrs).Step 2. Measure absorbance at A562 nm using an ELISA reader (540-590 nm filters may also be used, the absorbance valuesmay change slightly but the assay remains unaffected). Calculate concentration of protein in your sample from thestandard curve.General InformationBCA (bicinchoninic acid) Protein Assay is one of the most popular protein assays. It is compatible with many detergents andtherefore it provides an accurate determination of protein concentration in most sample types. Compared to most dye-bindingmethods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor proteincontamination in industrial applications.Advantages of ADI’s Ultra BCA Protein Assay.Features ADI Kit CompetitorBroad range (0.2-12 g) and Enhanced Sensitivity (~0.2 g) Yes NoIndustry’s first Ready to use Pre-coated Pre-dispensed standard strips (no errors, save Yes Notime, cost effective, and most convenient)Dual Standards (BSA and Bovine IgG) with every kit allows better sample compatibility Yes Buy SeparateMost Compatible with common buffers and detergents (Am. Sulphate=25mM; Glycine, pH,2.8=100 mM; Imidazole, pH 7.0=50mM; MOPS=100mM; RIPA buffer; NaCl=1 M;Yes Yes/NoTris=250mM; NP-40=5%; SDS=5%; TritonX-100=5%; Tween-20=5%; DTT andmercaptoethanol=1mM; DMF=10%; Sucrose=40%; Urea=3M; Glycerol=10%; DMSO=10%; &Many other reagents !!"#$%&!'() *+#(,)+,*-+,+#./$(,)+,*-+!!#0$111!'

Ordering information-Complete BCA Protein KitsCatalog# ProdDescription SIZEUltra BCA Protein assay kit (96 tests); with dual stds (Ready to use pre-coated BSA 1 kitStds, a set of 12 strips, 0-12 g), 1 vial of BGG at 1 mg/ml & 1 Protein assay plate (96wells)Ultra BCA Protein assay kit (480 tests); with dual stds (Ready to use pre-coated BSA 1 kitStds, a set of 12 strips, 0-12 g), 1 vial of BGG at 1 mg/ml & 5 Protein assay plates (480wells)Ultra BCA Protein assay kit (96 tests); with dual stds (Ready to use pre-coated BGG 1 kitStds, a set of 12 strips, 0-12 g), 1 vial of BSA at 1 mg/ml & 1 Protein assay plate (96wells)Ultra BCA Protein assay kit (480 tests); with dual stds (Ready to use pre-coated BGG 1 kitStds, a set of 12 strips, 0-12 g), 1 vial of BSA at 1 mg/ml & 5 Protein assay plates (480wells)BCAB-610BCAB-710BCAG-810BCAG-910Accessories available as separate itemsPBSA-5UPBGG-5UBSA-BGG-1BCAP-5Ready to use pre-coated, stabilized, Bovine serum albumin (BSA) Stds plates for UltraBCA protein assay kit, a set of 12 strips/plate, 0-12 ug), 5 plates/pkReady to use pre-coated, stabilized, Bovine Gamma Globulin (BGG) Stds plates forUltra BCA protein assay kit, a set of 12 strips/plate, 0-12 ug), 5 plates/pkBovine Serum Albumin (BSA) and Bovine Gamma Globulin (BGG) standard set proteintest kit (1 mg/ml, 1 ml each)Pre-optimized blank ELISA strip plates (96 wells, 8 wells/strips) for 480 tests for BCAprotein assays1 pk1 pk1 pk1 pkAdvantages of ADI’s Ultra BCA Protein Test Kits• BCA based protein test are most compatible with common buffers and reagents• ADI Ultra BCA Protein Test Kits offer most sensitivity with a range of 0.2-12 g (1-80 g/ml)• Short incubation time at 37 o C (Adaptable for room temp or longer incubation time toenhance sensitivity of the tests according to user’s needs• ADI kits supplied with Dual BSA and BGG standards for better sample compatibility• ADI kits have at least 1 yr shelf life• Convenient pack size of ~480 tests for most users for cost savings and prevent waste orexpiration of bulk reagents. We can supply bulk packs for special users.• ADI Kits available with several convenient selection of standards♦ Stock Standards (BSA or BGG) at 1 mg/ml (diluted at user end)♦ Industry first “Pre-coated Standards (BSA or BGG) Strip Plates ” for most convenientand error free protein tests. No need to dispense the standards, higher reproducibility.“Pre-coated Standards Strip Plates” is copy righted and trade mark of ADI Inc, USA. Patent Pending. !!"#$%&!'() *+#(,)+,*-+,+#./$(,)+,*-+!!#0$111!'