Jessica Lamb, University of Cambridge - MMS Conferencing
Jessica Lamb, University of Cambridge - MMS Conferencing
Jessica Lamb, University of Cambridge - MMS Conferencing
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Major Processes-. /. '())*+)") J. <strong>Lamb</strong>BioreceptorTransducerAmplifierMicroelectronics,& )) &))) )(*
The Glucose Biosensorn !! 01 Electrode2e-FerroceneFerroceniumGox oxGOx redGlucoseGluconolactoneCurrent1e-58e-66e-64e-62e-60-2e-6 -0.1 0.0 0.1 0.2 0.3 0.4 0.5E / (V vs Ag|AgCl)n ' n n 2334 4 J. <strong>Lamb</strong>
A New Approachn2! 5 ELECTRODE 2!'', J. <strong>Lamb</strong>n 6!! ,).+.7..).+.7.. . .0-#891:3 ..).);. .0-#8
J. <strong>Lamb</strong>Macrocyclic Scaffoldsn " 5(("n ,!3 nnn !"!#!$!! !' &n !n ; n OOOOOO-8 "9NHHNHNElectrodeNHNH HNMNH HNLigand
Octahedral Iron Cyclam Complexesn01 1 =NH HNNH HNA /A; Guilard et al., Dalton Trans., 1997NHHNNHFeNHClCl++NHNHClFeClNHHN+n0((1 1 /NH HNNH HN+;* !NNNN +)2@/?2N+NN N+.2Br -+;?2N HNNH NFeCl 2Dry DMFNHHNNFeNClCl2 .).), ,.>.. .0-##81 J. <strong>Lamb</strong>
Octahedral Iron Cyclam ComplexesnI (uA)2! n B01 / C6$.-/
Scaffold Functionalisation withBiorecognition Elementnn nnnnH (' OH NH 2 J. <strong>Lamb</strong>
Tyramine Scaffold SynthesisNNNNBrOOHHafliger & Kaden, Helv+;) , ;);/HO#$I )/)=; ; Helv. Chim. Acta, , (1979)OHONNONNO%&%OOHOHNH 2OHHOHOHNONHN NNiN NOOONHHNOH)*%+, - OH#!#'(/A./ ?HOHOHNONHNNO%#!#'(+2+2-;)-= ) ))'2NNOONHHNOHOH J. <strong>Lamb</strong>
3T Biocharacterisationn 6!HOHOORHORORabsorbance0.40.30.20.10Oxidation <strong>of</strong> 3T by Tyrosinaset=1t=3t=5t=7t=9t=11t=13t=15t=20t=25t=30t=35A,( ,(270 370 470 570wavelength§ λ ! 6 /#/%$#.%$#'%%%%&%%$#.%$#'%%%%&% J. <strong>Lamb</strong>
3T Biocharacterisationn " HOHOHOHOHOHOHNHNHNONHONHONHNNONNONNONNONNONNOONHONHONHHNHNHNOHOHOHOHOHOHRate (abs/min)0.040.030.020.010.00Competitive Tyrosinase Assay***3T 3T +TyramineAb*3T +ControlAbTyramineAb J. <strong>Lamb</strong>
Scaffold Immobilisation§ , =A § HOOONHN NNiNNHNOOOH§ ' § * !! NHHNHONNNNOH SSS S S S SSS J. <strong>Lamb</strong>
M(3T): A Novel Enzyme Substraten 330=10=1OOHOHNONNMNNOHNOHOOHNONNMNNOHNOOHONHOONHOHONHOONHOHOOP OOHOHO P OOHHNONHN NMN NO OONHHNOO P OHOHOO P OHOH3JHOHOHNONHN NMN NO OONHHNOHOH J. <strong>Lamb</strong>
OOHNH NHNiNH NHNH NNiNH NHSummaryNHHNNHFeNHClClNHHNNFeNClCln,!!n 4n !! n !n K/$$D !n" n n 0=15+0=10 ? 1 /n F n " =n =HOHONHNHNOONHHNNNONHNNOOONHHNHNSONHOHOH J. <strong>Lamb</strong>NH HN
AcknowledgementsnnnJ.;*; H ' F *:3H LM J. <strong>Lamb</strong>
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