Thermo Scientific PikoReal Real-Time PCR System - Fisher Scientific

More documents

Recommendations

Info

16 Genomics DreamTaq Green DNA Polymerase • High yields of PCR products without optimization, MgCl2 included into the buffer. • Higher sensitivity compared to conventional Taq DNA polymerase. • Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA. • Robust amplification of difficult templates. Cat.No Alt.No Description Size 257101340 EP0711 200 u 257101350 EP0712 DreamTaq Green DNA Polymerase, 500 u 5 u/μl supplied with 10X DreamTaq 257101360 EP0713 Green Buffer 5x500 u 257101370 EP0714 20x500 u 257101300 EP0701 257101310 EP0702 DreamTaq DNA Polymerase, 5 u/μl 500 u 257101320 EP0703 supplied with 10X DreamTaq Buffer 5x500 u DreamTaq Green PCR Master Mix (2X) illustra PuReTaq Ready-To-Go PCR Beads illustra PuReTaq Ready-To-Go PCR Beads are a newly designed, premixed, predispensed reactions designed for performing standard PCR. The single-dose, ambient-temperaturestable beads ensure greater reproducibility between reactions, minimize pipetting steps and reduce the potential for pipetting errors and contamination. The only additional reagents are water, template DNA and primers. The beads are provided in either 0.5 ml or 0.2 ml tubes that are compatible with most thermal cyclers. Cat.No Alt.No Description Size 230015050 27-9557-01 illustra PuReTaq Ready-To-Go PCR Beads (0.2 ml tubes/plate) 230015060 27-9558-01 illustra PuReTaq Ready-To-Go PCR Beads (0.5 ml tubes) 230015040 27-9559-01 illustra PuReTaq Ready-To-Go PCR Beads (0.2 ml hinged tube with cap) 200 u 257101330 EP0704 20x500 u • Convenient – DreamTaq Green DNA Polymerase in a ready-to-use master mix. • High yields of PCR products without optimization. • Higher sensitivity compared to conventional Taq DNA polymerase. • Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA. • Robust amplification of difficult templates. Part of Thermo Fisher Scientific Part of Thermo Fisher Scientific Cat. nr Alt.No Description Size 257107300 K1081 DreamTaq Green PCR Master Mix (2X) supplied 4x1.25 ml 257107310 K1082 with Water, nuclease-free 20x1.25 ml 257107280 K1071 DreamTaq PCR Master Mix (2X) 4x1.25 ml 257107998 K1072 supplied with Water, nuclease-free 20x1.25 ml 96 reactions 100 reactions 96 reactions T 22 95 59 59 F 22 95 59 40 qPCR Reagents DyNAmo SYBR Green and Probe qPCR Kits DyNAmo qPCR Kits are ready-to-use 2x master mix for sensitive, quantitative real-time PCR. These master mixes contain a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The modified DNA polymerase in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. • Sensitivity: Optimized 2x master mix allows reproducible detection of low-copy number templates. • Speed: More efficient amplification coupled with increased polymerase processivity results in earlier C(t)s and shorter overall qPCR reaction times. • Convenience: 2x master mix, one tube only. ROX passive reference dye is provided in a separate vial in some kits. • Broad dynamic range: DyNAmo qPCR Kits detect as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude. Cat.No Alt.No Description Pkg.Size 252400010 F-400S DyNAmo SYBR® Green qPCR Kit 252400020 F-400L DyNAmo SYBR® Green qPCR Kit 252400030 F-400RS DyNAmo SYBR® Green qPCR Kit with ROX 252400040 F-400RL DyNAmo SYBR® Green qPCR Kit with ROX DyNAmo HS SYBR® Green qPCR Kit Part of Thermo Fisher Scientific 40 x 50µl reactions 200 x 50µl reactions 40 x 50µl reactions 200 x 50µl reactions 252450010 F-450S DyNAmo Probe qPCR Kit 40 x 50µl reactions 252450010 F-450L DyNAmo Probe qPCR Kit 200 x 50µl reactions Part of Thermo Fisher Scientific DyNAmo HS SYBR® Green qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The 2x master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. • Specificity: DyNAmo HS SYBR Green master mix minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers during reaction setup. • Sensitivity: Optimized master mix allows reproducible detection of low-copy number templates. • Broad dynamic range: DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude. • Convenience: 2x master mix, one tube only. ROX passive reference dye provided in a separate vial. Cat.No Alt.No Description Pkg.Size 252410010 F-410S DyNAmo HS SYBR® Green qPCR Kit 40 x 50µl reactions 252410020 F-410L DyNAmo HS SYBR® Green qPCR Kit 200 x 50µl reactions
qPCR Reagents DyNAmo Flash qPCR Kits DyNAmo Flash SYBR® Green qPCR Kit and DyNAmo Flash Probe qPCR Kit offer enhanced performance for real-time qPCR. These kits provide sensitive and reproducible detection of target DNA with shorter run times in both fast and conventional instruments. • Extremely fast protocols: combined annealing and extension step of only 15 s • Specific and sensitive detection of a wide range of template concentration • Included dUTP allows the use of UNG for prevention of carry-over contamination • Convenient 2x master mix and optimized protocols • Validated with all major real-time instruments • Licenced for qPCR • qPCR results faster without compromising performance • Reliable and robust amplification • High specificity and sensitivity • High signal intensity Cat.no Alt.No Description Pkg.Size 252415010 F-415S DyNAmo Flash SYBR® Green qPCR Kit 100 x 20µl rxns 252415020 F-415L DyNAmo Flash SYBR® Green qPCR Kit 500 x 20µl rxns 252455010 F-455S DyNAmo Flash Probe qPCR Kit 100 x 20µl rxns 252455020 F-456S DyNAmo Flash Probe qPCR Kit 500 x 20µl rxns Maxima SYBR Green qPCR Master Mix (2X), ROX Solution provided Part of Thermo Fisher Scientific Part of Thermo Fisher Scientific Maxima SYBR Green qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR on most real-time PCR machines. It detects low copy number targets and has a wide linear range. The ROX solution is provided in a separate tube for use with machines that requires ROX as a passive reference dye. Cat.No Alt.No Description Pkg.Size 257106980 K0251 Maxima SYBR Green qPCR Master Mix (2X), ROX Solution provided 257106990 K0252 Maxima SYBR Green qPCR Master Mix (2X), ROX Solution provided Real-time PCR FRA 782 FRA 1113 200 reactions 1000 reactions Real-time PCR (also known as qPCR) has quickly become the gold standard technique for quantitation of nucleic acids in biological samples. Its unsurpassed sensitivity has made it possible to use much smaller sample quantities than ever before. The technology is based on the nobel prize winning Polymerase Chain Reaction which amplifies a specific DNA fragment DyNAmo ColorFlash qPCR Kits Finnzymes’ new DyNAmo ColorFlash qPCR Kits are a superior choice for fast, quantitative real-time analysis. These kits offer excellent performance in detection and quantification of DNA sequences from various sources. DyNAmo ColorFlash qPCR Kits are designed to ease qPCR setup and minimize errors by providing a simple way to track pipetting of reaction components. • Minimized risk of pipetting errors during reaction setup • Also beneficial when using white reaction vessels • Extremely fast protocols due to combined annealing and extension step of only 15 s • Specific and sensitive detection of a wide range of template concentrations • dUTP included in the 2x Master Mix allows the use of UNG for prevention of carry-over contamination • Licensed for qPCR + = Master mix Sample in buffer qPCR reaction mix Part of Thermo Fisher Scientific Cat.no Alt.No Description Pkg.Size 252416010 F-416S DyNAmo ColorFlash SYBR® Green qPCR Kit 100 x 20µl reactions 252416020 F-416L DyNAmo ColorFlash SYBR® Green qPCR Kit 500 x 20µl reactions 252456010 F-456S DyNAmo ColorFlash Probe qPCR Kit 100 x 20µl reactions 252456020 F-456L DyNAmo ColorFlash Probe qPCR Kit 500 x 20µl reactions Maxima Probe qPCR Master Mix (2x), ROX solution provided Part of Thermo Fisher Scientific Maxima Probe qPCR Master Mix (2x)is a ready-to-use solution optimized for quantitative realtime PCR and two-step real-time RT-PCR on most real-time PCR machines. The ROX solution is provided in a separate tube for use with machines that requires ROX as a passive reference dye. The use of Maxima Probe qPCR Master Mix in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Cat.No Alt.No Description Pkg.Size 257107000 K0261 Maxima Probe qPCR Master Mix (2x), ROX solution provided 257107010 K0262 Maxima Probe qPCR Master Mix (2x), ROX solution provided 200 reactions 1000 reactions exponentially to huge amounts that can readily be detected by for example fluorescent staining on gels. In real-time PCR, the amount of nucleic acid is monitored during the amplification process and one can obtain information about the quantity of a specific gene. Different technologies to detect the fluorescence exist, including non-specific dyes such SYBR Green or DNA-probes such as TaqMan probes. E fisher.no@thermofisher.com W www.fishersci.no 17
Page 1 and 2: Genomics ONE SOURCE. INFINITE SOLUT
Page 3 and 4: Specifications and Ordering Informa
Page 5 and 6: Accessories separately available Th
Page 7 and 8: VIAFLO 96 Unique Multichannel micro
Page 9 and 10: Kjører dere akkrediterte analyser?
Page 11 and 12: PCR Reagents Phusion® High-Fidelit
Page 13 and 14: In a hurry? Use Direct PCR from Fin
Page 15: Thermo-Start Taq DNA polymerase For
Page 19 and 20: PCR-Products, BRAND BRAND has signi
Page 21 and 22: KingFisher Flex - Thermo Scientific
Page 23 and 24: Gel Electrophoresis and Analysis Ho
Page 25 and 26: Vi har også en helt ny brosjyre me
Page 27 and 28: Fast Digest® Electrophoresis • T

Thermo Scientific PikoReal Real-Time PCR System - Fisher Scientific

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?