Thermo Scientific PikoReal Real-Time PCR System - Fisher Scientific

Genomics 

ONE SOURCE. INFINITE SOLUTIONS. ® 

PCR & qPCR 

Husk kampanjekode Genomics2011

2 

Genomics 

Thermo Scientific 

PikoReal Real-Time 

PCR System 

The Thermo Scientific PikoReal 

Real-Time PCR System is a big 

instrument in a tiny box. It offers 

excellent qPCR performance to a 

price point where you do not have 

to share. 

Real-Time, Any Time – Now within 

your reach! 

The Thermo Scientific PikoReal Real- 

Time PCR System is light, quiet and has 

the smallest footprint in the market, 

thereby making it ideal for personal 

use. The system is available in two 

different configurations: PikoReal ® 

24-well system for use with 24-well 

Piko PCR Plates and standard 8-well 

strips, and PikoReal 96-well system 

for use with 96-well Piko PCR plates. 

Piko PCR Plates are only ¼ the size of a 

conventional microplate, but they still 

maintain industry standard spacing and 

sample capacity. They are compatible 

with standard multichannel pipettes, 

reagent dispensers and automated 

liquid handling systems. Due to its small 

size, extremely low energy consumption 

and durable design, PikoReal system is 

also suitable for field applications. 

Superior thermal performance 

The unique block design (patent 

pending) of PikoReal system combined 

with precise temperature control brings 

confidence to your qPCR. The superior 

thermal performance ensures that your 

quantification results are reliable and 

reproducible. Five optical channels 

facilitate multiplexing. 

T 22 95 59 59 F 22 95 59 40 

Product Specifications 

Stand-alone or PC control 

PikoReal system is controlled by 

PikoReal Software, which allows 

you to remotely monitor any qPCR 

experiment on-line from your own 

computer. Alternatively, you can save 

the qPCR protocol on a USB stick and 

load it on to the PikoReal instrument 

for stand-alone use. PikoReal software 

includes modules for absolute and 

relative quantification, melting curve 

analysis and genotyping (a module for 

HRM analysis will be available in 2011). 

Advanced algorithms provide effortless 

data normalization without the need for 

an external reference dye. 

Reduced plastic and reagent 

consumption 

The unique design of the heating 

block and the small plate format allow 

significant savings both in plastics and 

reagent consumption. This reduces 

costs of your research and produces 

less plastic waste. 

FRA 

110.000 

Not available in US, Canada, Brazil, UK, Germany, Austria, Switzerland, Italy, Spain, France, Belgium, the Netherlands, 

Luxemburg, Denmark and Sweden.

Specifications and Ordering Information 

Life Science 

Research 

Thermo Scientific PikoReal Real-time PCR System 

Thermal Block 

Block formats 24-well, 96-well (not interchangeable) 

Sample volume 10 – 50 µl (PikoReal 24), 5 – 20 µl (PikoReal 96) 

Consumables 24-well or 96-well Piko PCR Plate 

Max heating rate >5°C/sec 

Max cooling rate 4.5°C/sec 

Temperature range 4 – 99.9°C 

Temperature accuracy ±0.2°C 

Temperature uniformity ±0.3°C at 95°C 

Heated lid 

Temperature range 30 – 110°C 

Control Automatic temperature and pressure setting 

Optics 

Excitation 5 LEDs 

Excitation range 475 – 640 nm 

Detection CCD 

Detection range 

Detection channels: 

520 – 740 nm 

Exitation (nm) 

Emission (nm) 

475–500 

520–550 

515–535 

557–590 

570–590 

615–650 

600–640 

666–740 

475–500 

520–590 

Multiplex Up to 4 targets 

HRM Available 2011 

Dynamic range 10 orders of magnitude 

Sensitivity 1 copy 

Scan time for 4 multiplexing channels

4 

Genomics 

Thermocyclers 

Thermo Scientific Piko Thermal Cyclers 

deliver high performance in a compact package. Although merely half the size of other 

instruments, the Piko® Thermal Cycler meets the highest criteria in performance and is able 

to complete a PCR protocol in less than 15 minutes. This is achieved using novel technical 

advances that allow significant reduction in PCR run times and overall size of the instrument. The 

Piko Thermal Cycler is an ideal solution for both conventional and fast PCR applications. Due to 

its small size and low power consumption, the Piko Thermal Cycler is also conveniently portable 

for field use. 

Thermo Scientific Piko Thermal Cyclers offer you 

• Best-in-class thermal performance Consistent results from well-to-well due to extremely 

short settling times and high temperature uniformity across the block. 

• Time savings PCR in as little as 15 minutes enabled by fast ramp rates and quick settling 

times. 

• Reagent savings Repeatable yields from as little as 5 μL when using UTW vessels and heat 

sealers. 

• Space savings The smallest footprint available - fits into a tiny bench space. 

• Energy saving Short protocols and low wattage requires only ¼ the power consumption of 

typical thermal cyclers. 

• Repeatable sealing to prevent sample evaporation Automatic heated lid provides consistent 

and tight sealing from run-to-run. 

Two formats: 24- and 96-well blocks 

Piko Thermal Cyclers are available in two different block configurations: 24-well and 96-well. 24well 

cyclers accept all standard low profile single tubes, and 8-tube strips with flat caps as well 

as 24-well Piko PCR Plates. The 96-well instrument utilizes 96-well Piko PCR Plates, which are 

only ¼ the size of a conventional microplate. Despite the small size, all Piko PCR Plates maintain 

industry standard well spacing and sample capacity. The 24-well and 96-well Piko PCR Plates 

are compatible with standard Thermo Scientific multichannel pipettes, Multidrop® reagent 

dispensers and the Versette automated liquid handling system. 

High yields and uniform results in less than 15 minutes 

The best-in-class thermal performance of Piko Thermal Cyclers means significant reduction 

of incubation times in most PCR protocols. This is due to the fast ramp rates, near-perfect 

temperature uniformity and incredibly short block settling time. It only takes 1 second for all the 

samples to reach temperature equilibrium. 

A 400 bp amplicon from lambda DNA was amplified with an ultra-fast PCR protocol on a 24-well Piko 

Thermal Cycler using UTW reaction vessels and Phusion Flash High-Fidelity PCR Master Mix. 5 μl aliquots 

of each 15 μl reaction were run on an agarose gel. The following PCR protocol was used: 25 cycles of [98°C 

for 0 sec, 72°C for 0 sec], 72°C for 10 sec. Total cycling time was about 10 min. 

T 22 95 59 59 F 22 95 59 40 

Part of Thermo Fisher Scientific 

FRA 

29.900 

Green PCR 

Reduced consumption – Enhanced performance 

The unique design of the heating block and small plate format allow significant savings both in 

energy and plastics consumption. This helps reduce the environmental impact of your PCR. 

Significant savings in energy and plastics consumption. A) Total estimated annual energy consumption of 

the 96-well Piko Thermal Cycler vs. conventional 96-well cycler. B) Estimated annual plastics consumption 

using 96-well Piko PCR Plates vs. conventional 96-well microplates. Both calculations are based on 400 

PCR runs per year.

Accessories separately available 

Thermo Scientific Piko PCR Plates 

Thermo Scientific Piko PCR Plates guarantee reliable results with routine and ultra-quick 

PCR protocols. The vessel walls are half the thickness of conventional thinwalled tubes. This 

improves thermal transfer between the heating block and sample allowing shorter incubation 

times without compromising the consistency of results. Plate frames are available to assemble 

Piko PCR Plates into standard SBS format plate size. 

Thermo Scientific Piko Plate Illuminator - Simplify sample 

loading 

The Thermo Scientific Piko Plate 

llluminator is a state-ofthe-art plate rack 

that provides a simple way to track the 

loading of reactants by illuminating the 

target well(s) from below with white 

LED lights. 

Thermo Scientific Piko Microcentrifuge 

The Thermo Scientific Piko Microcentrifuge 

is a compact centrifuge for spinning 

down samples. It is compatible with all 

0.2 ml tubes, 8-tube strips and Piko PCR 

Plates. The Piko Microcentrifuge has two 

interchangeable rotors. 

Piko Studio software 

The Thermo Scientific Piko Studio is a full-featured PC software that allows you to control the 

Piko Thermal Cycler remotely, backup, edit and transfer protocols. 

Technical specifications 

Piko Thermal Cycler 

Size Width 16 cm, depth 17 cm, height 23 cm 

Weight 4 kg (with external power supply) 

Power requirements 100–240 V input power 

Block configurations 24-well (well volume 0.225 ml, sample volume 

max 50 μl) and 96-well (well volume 0.05 ml, 

sample volume max 20 μl 

Max ramp rate > 5°C heating and > 4.5°C cooling 

Typical thermal uniformity +/- 0.3°C 

Thermal accuracy +/- 0.2°C 

Thermal range 0°C - 99.9°C 

User interface Semi-graphical and list mode programming 

Number of programs Over 1000 

Heated lid Fully-automated and motorized function 

Warranty 2 years parts and labor 

Cat.No Alt.No Description Pkg.Size 

Piko Thermal Cyclers 

253230249 14-230-249 Piko Thermal Cycler, 24-well 1 

253230250 

Piko Studio 

14-230-250 Piko Thermal Cycler, 96-well 1 

253200001 PST0001 1 user 

253200005 PST0005 up to 5 users 

253200010 PST0010 up to 10 users 

Piko Plate Illuminator 

253302496 PIP2496 Piko Plate Illuminator 1 

Piko Microcentrifuges 

253400012 MCP0012 Piko Microcentrifuge with 2 rotors 1 

Piko PCR Plates and Frames 

253500240 SPL0240 24-well Piko PCR Plate, clear 200 plates 

253500960 SPL0960 96-well Piko PCR Plate, clear 200 plates 

253600241 SFR0241 Plate Frame for 24-well Piko PCR, white. Holds 

4 plates. 

50 frames 

253600961 SFR0961 Plate Frame for 96-well Piko PCR, white. Holds 

4 plates. 

UTW® PCR tubes with flat caps 

253700010 TUC0010 UTW® individual tube with attached, flat 

optical cap, clear 

50 frames 

960 tubes 

Sealers 

253800021 HSF0021 Heat sealing film, opaque, Piko size 400 sheets 

253800020 ASF0020 Optical adhesive film, cryo-storage 

compatible, Piko size 

400 sheets 

253801080 TCS1080 Flat 8-cap strips, optical 120 strips 

E fisher.no@thermofisher.com W www.fishersci.no 5

6 

Genomics 

Thermo Scientific 

Arktik Thermal Cycler 

The Thermo Scientific Arktik Thermal Cycler is a reliable and flexible PCR instrument for the 

everyday requirements of any laboratory. It is suitable for a wide range of applications from 

individual reactions to high-throughput projects. 

Features 

• Interchangeable blocks with excellent thermal precision 

• Dual 48-well block for multi-user option 

• Broad and accurate temperature gradient 

• Reliable and easy to use 

• Low noise level 

• Accepts almost all standard PCR plastics 

Flexibility with interchangeable blocks 

Interchangeable blocks allow the Arktik Thermal Cycler to be quickly and easily transformed 

from a 96-well to a 384-well PCR instrument. With the dual block the instrument operates as 

two 48-well units, enabling two independent protocols to be performed simultaneously. The 

blocks feature a patent pending heat sink design that improves the speed and thermal uniformity 

of the system. 

Broad and accurate temperature gradient speeds up 

protocol optimization 

Tedious temperature optimization can be simplified to a single experiment with the Arktik 

Thermal Cycler’s advanced gradient feature. The gradient range is broad – up to 30°C – and 

accurate, allowing easy temperature optimization. 

Reliable and quiet instrument for demanding use 

State-of-the-art Peltier technology employed in heating and cooling of the blocks ensures that 

the instrument maintains its high level of performance in demanding use. An overtightening 

protection system in the heated lid simplifies setting the correct pressure for PCR vessels used. 

The low noise level of the instrument is an additional benefit when the instrument is placed in 

the laboratory working space. 

Ease-of-use 

Ease-of-use is the key feature of the Arktik Thermal Cycler’s user interface. The protocol is 

displayed graphically, and the programming is simple with intuitive menus. 

FRA 

36.900 

T 22 95 59 59 F 22 95 59 40 

Technical Specification 

Block configurations 96 x 0.2 ml, 384 x 0.03 ml, 2 x 48 x 0.2 ml (dual block). 

Interchangeable. 

Gradient range Max 30°C 

Max ramp rate 3.0°C/s 

Thermal uniformity +/- 0.4°C at 90°C 

Thermal accuracy +/- 0.3°C 

Thermal range 4-99.9°C 

Heated lid Manually adjustable. Over-tightening protection system. 

User interface Semi-graphical 

Number of programs 4950 

Size Width 29 cm, depth 38 cm, height 29 cm 

Weight 10.5 kg 

Power requirements 100-240 V input power, 50-60 Hz, 600 W 

Communications Ethernet, USB memory port 

Warranty 1 year 

Certifications CE 

Cat.No Alt.No Description 

253100001 Arktik Thermal Cycler base unit, with gradient 

253100002 Arktik Thermal Cycler base unit, without gradient 

253100096 96-well single block 

253100384 384-well single block 

253104848 Dual block (2 x 48 wells) 

Part of Thermo Fisher Scientific

VIAFLO 96 Unique 

Multichannel microplate pipetting is a growing task because 

of the need to increase productivity and improve 

the quality of the data obtained by increased numbers 

of replicates. In addition to the large investments that 

are traditionally required to obtain such instrumentation, 

many customers are also concerned about the complexities 

associated with using an automated system. 

INTEGRA’s VIAFLO 96 introduces a totally new 

concept in 96 well pipetting, which is affordable and 

simple to use. It renders 96 channel pipetting as easy 

as manual single channel pipetting. 

VIAFLO 96 performs all tasks easily in multiwell format, 

just as a standard pipette would in a single tube. 

Applications may include: 

� Replication or reformatting of plates 

� Transfer of reagents and compounds from plate to 

plate or within plates 

� Gentle and convenient dissolution or re-suspension 

of solid compounds 

� Fast execution of serial dilutions by columns 

� Increment dispensing to multiple plates 

VIAFLO 96 is used like a hand-held pipette. The 

movement and positioning of the 96 channel pipette 

is supported by a servo assisted steering mechanism 

which allows fast, precise and stress free multichannel 

pipetting. 

Just by moving the assisted pipetting head between 

two plate positions, as you would move a hand-held 

pipette, you are able to simply perform 96 channel 

pipetting! 

Therefore, no special skills or training are required to 

work with this small bench top unit. Immediately 

benefit from a whole new level of usability, ergonomic 

comfort and productivity. 


8 

Genomics 

Vi validerer og sertifiserer PCR maskiner. Målinger 

viser at PCR maskiner ikke alltid holder hva de lover. 

De viktigste parameterne for PCR maskiner er jevnhet og nøyaktighet. 

Det betyr at forskjellen i blokken er innenfor det som er spesifisert 

og at temperaturen er riktig. Displayet viser ikke alltid riktig 

temperatur, det er som regel bare en indikasjon på temperaturen. 

Med vårt kalibrerte utstyr sjekker vi 15 forskjellige brønner i blokken på PCR maskinen. 

Hvert målepunkt logger temperaturen 2 ganger i sekundet mens din PCR maskin kjører en 

fastlagt protokoll som tester følgende temperaturer: 95, 90, 70, 60, 50 og 30 grader C. 

Du får en rapport som viser hvordan din maskin klarer kravene til nøyaktighet, jevnhet og 

hastighet. Dersom det viser seg at maskinen ikke klarer de spesifikasjonene som gjelder 

for maskinen, kan du vurdere å kontakte leverandøren. 

Validering koster fra Kr. 2.500,- eks. MVA og er en fornuftig 

investering når man tar i betraktning hva en PCR maskin koster 

og utgiftene til reagenser og plast. 

Flere medisinsk tekniske avdelinger på norske sykehus har kjøpt inn 

Driftcon utstyr for testing av de forskjellige PCR instrumentene de har. 

Et komplett Driftcon system koster fra ca. Kr. 50.000,- eks. MVA. 

Ta kontakt så sender vi deg et tilbud tilpasset ditt behov. 

Flere av våre kunder har sluppet 

å kjøpe ny PCR maskin etter at vi 

har validert den og funnet 

løsninger på deres problemer. 

T 22 95 59 59 F 22 95 59 40

Kjører dere akkrediterte analyser? 

MTAS er en godkjent metode for 

testing av PCR-instrumenter iht. 

ISO17025. 

Årlig må du inn til EU-kontroll med 

bilen din, for å være sikker på at den 

er i orden. 

Har du noen gang kontrollert at din PCR-maskin 

faktisk gir deg det som produsenten har lovet deg, 

og at kvaliteten er innenfor de rammer dere ellers 

jobber under? 

Vi testet 4 PCR-instrumenter av samme type fra en av markedslederne. 

Det var lovet jevn temperatur i 96-platen og raske temperaturendringer. 

Vår validering viste at kun 1 av de 4 maskinene 

klarte produsentens egne krav til jevnhet og ingen av dem 

klarte produsents løfte om temperaturendring. 

Produsentens spesifikasjoner var: Valideringen viste: 

Jevnhet over hele blokken ved 95 o C: 1.00 o C 2,93 o C 

Nøyaktighet ved 95 o C : +/- 0.25 o C +/-1,55 o C 

Hastighet ved oppvarming: 5 o C/sek. 1,92 o C/sek 

Hastighet ved nedkjøling: 5 o C/sek. 1,92 o C/sek 

Stoler du på de dataene som produsenten har oppgitt for 

ditt PCR-instrument ? 

Valideringen tar ca. 1 time og gir i en 16 siders rapport som er 

godkjent for bruk i interne kvalitetssystemer og rapportering 

mot offentlige kontrollinstanser. Alle målinger er sporbare. 

Brukerne skreddersyr nå sine protokoller til de faktiske 

verdiene deres maskiner gir, og de unngår mislykkete 

forsøk pga. avvik i maskinene. 

Burde du også vurdere å få validert din maskin? Du sparer raskt 

inn hva det koster når du ser på hva reagensene koster og din 

egen verdifulle tid. 

Validering av 1 instrument med Driftcon koster kr 2.500,-. 

Sertifisering av 1 instrument med MTAS koster kr. 4.500,-. 

Har du flere maskiner, gir vi deg gjerne et tilbud. 

Her ser vi en kraftig ”overshoot” på 

8 o C før man kommer ned til 90 o C. 

Her ser vi en kraftig ”undershoot” på 

7 o C før man kommer opp til 50 o C. 

Prisene er gyldige til 30/12-2011. Prisene er eks. MVA, ex works Oslo. 


GeneJET RNA Purification Kit 

The GeneJET RNA Purification Kit is a simple and efficient system for purification of total 

RNA from mammalian cultured cells,tissue, human blood cells, bacteria and yeast. The 

kit utililzes a silica-based membrane technology in the form of a convenient spin column, 

eliminating the need for tedious cesium chloride gradients, alcohol precipitation or toxic 

phenol-chloroform extractions. RNA molecules longer than 200 nucleotides can be isolated 

with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality 

purified RNA can be used in a wide range of downstream applications including RT-PCR, TTqPCR, 

Nothern blotting and other RNA based analyses. 

• Universal – can be used for both cell and tissue samples from a wide range of sources: 

mammalian blood, mammalian cell cultures, mammalian tissues, yeast, bacteria. 

• Efficient – high yields of total RNA. 

• Fast – less than 15 min procedure following cell lysis. 

Cat.No Alt.No Description Size 

257107220 K0731 

50 preps 

GeneJET RNA Purification Kit 

257107230 K0732 250 preps 

GeneJET Genomic DNA 

Purification Kit 

The GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification 

of high quality genomic DNA from whole blood, various mammalian cell culture or tissue 

samples and bacteria. The purified genomic DNA is up to 50 kb in size with dominating 

fragments of 20-30 kb. Isolated DNA can be directly used for PCR, Southern blotting and 

enzymatic reactions. 

• Universal – can be used for both cell and tissue samples from a wide range of sources: 

mammalian blood, mammalian cell cultures, mammalian tissues, yeast, bacteria. 

• Efficient – high yields of high molecular weight genomic DNA. 

• Fast – 20 min procedure following cell lysis. 


257107200 K0721 

50 preps 

GeneJET Genomic DNA Purification Kit 

257107210 K0722 250 preps 

GeneJET Gel Extraction Kit 

The GeneJETGel Extraction Kit is designed for rapid and efficient purification of DNA 

fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer. 

The kit utililzes a silica-based membrane technology in the form of a convenient spin column. 

The kit can be used to purify DNA fragments from 25 bpto 20 kb in size with recovery rates 

up to 95%. The kit has a binding capacity of up to 25µg of DNA and can process up to 1 g of 

agarose gel. The entire procedure takes 15 min and the isolated DNA is ready to use in all 

common downstream applications including ligation, restriction digestion, PCR, sequencing 

and labeling. 

• Highly efficient – up to 95% recoveries in the range of 100 bp – 10 kb. 

• Fast – procedure takes only 15 min. 

• Convenient – spin columns are capped and assembled with collection tubes. 





257107160 K0691 

50 preps 

GeneJET Gel Extraction Kit 

257107170 K0692 250 preps 

FRA 

1581 

FRA 

952 

FRA 

540 

GeneJET PCR Purification Kit 

The GeneJETPCR Purification Kit is designed for rapid and efficient purification of DNA from 

PCR and other enzymatic reactions. The kit utililzes a silica-based membrane technology in 

the form of a convenient spin column. The kit removes primers,dNTPs, unincorporated labeled 

nucleotides, enzymes and salts from PCR and other mixtures. The GeneJet PCR purification 

kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery raes up 

to 100 %. The purification columns has a total binding capacity of up to 25µg of DNA and 

the procedure takes only 5 minutes. The purified DNA can be used in common dovnstream 

applications such as sequencing,restriction digestion, labeling, ligation, cloning, in vitro 

transcription, blotting or in situ hybridiztion. 

• Highly efficient – 90-100% recoveries in the range of 100 bp – 10 kb. 

• Fast – procedure takes only 5 min. 

• Convenient – spin columns are capped and assembled with collection tubes. 

Cat. nr Alt.No Description Size 

257107180 K0701 

50 preps 

GeneJET PCR Purification Kit 

257107190 K0702 250 preps 

GeneJET Plasmid Kit 

The GeneJET Plasmid kits are designed for isolation 

of high quality plasmid DNA from recombinant 

E.coli cultures. Each kit recovers high yields of 

plasmid DNA. The kit utililzes a silica-based 

membrane technology in the form of a convenient 

spin column The purified plasmid DNA is ready for 

use in all molecular 

biology procedures 

such as restriction 

digestion, PCR cloning, 

transformation, 

automated sequencing, 

in vitro transcription 

and transfection. 

Kit Components: 

• GeneJET 

Spin Columns 

assembled with Collection Tubes 

• Resuspension Solution 

• Lysis Solution 

• Elution Buffer 

• Neutralization Solution 

• Wash Solution (concentrated) 

• RNase A Solution 

FRA 

441 


257107060 K0502 

50 preps 

GeneJET Plasmid Miniprep Kit 

257107070 K0503 250 preps 

GeneJET Plasmid prep kits guarantees 

reproducible and high quality results! 


FRA 

540 


PCR Reagents 

Phusion® High-Fidelity DNA 

Polymerase 


The schematic structure of Phusion® High-Fidelity DNA Polymerase. The double-strand DNA-binding 

domain (purple) is fused to a novel Pyrococcus-like proofreading enzyme (green) forming a unique highperformance 

polymerase - Phusion DNA Polymerase. 

The unique Phusion® DNA Polymerase offers performance that no other enzyme can match. 

The Phusion DNA Polymerase generates templates with an accuracy and speed unattainable 

with a single enzyme, even on your most difficult templates. The unique structure and 

characteristics of Phusion DNA Polymerase make it a superior choice for cloning, and sets 

a new standard for PCR performance: its error rate is 50-fold lower than that of Thermus 

aquaticus, and 6-fold lower than that of Pyrococcus furiosus - thus being the most accurate of 

available thermostable polymerases. Phusion DNA Polymerase possesses processivity 10-fold 

greater than Pyrococcus furiosus. 

Accuracy - The highest fidelity of any available thermostable polymerases 

Speed - Increased processivity allows shorter reaction times (extension 15-30 s/kb) 

Robustness - Fewer reaction failures and minimal optimization 

High yields - Increase product yields with minimal enzyme amounts 

Specificity - Hot start modification reduces non-specific amplification and primer degradation 

Cat.no Alt.No Description Pkg.Size 

252530010 F-530S Phusion® High-Fidelity DNA Polymerase 100U 

252530020 F-530L Phusion® High-Fidelity DNA Polymerase 500U 

252531010 F-531S Phusion® High-Fidelity PCR Master Mix with 

HF Buffer 

100 x 50µl rxns 

252531020 F-531L Phusion® High-Fidelity PCR Master Mix with 

HF Buffer 

252532010 F-532S Phusion® High-Fidelity PCR Master Mix with 

GC Buffer 

252532020 F-532L Phusion® High-Fidelity PCR Master Mix with 

GC Buffer 




252553010 F-553S Phusion® High-Fidelity PCR Kit 50 x 50µl rxns 

252553020 F-553L Phusion® High-Fidelity PCR Kit 200 x 50µl rxns 

Phusion® Flash High-Fidelity 

PCR Master Mix 

Phusion® Flash High-Fidelity PCR Master Mix is a 2x master mix enabling the use of 

extremely short PCR protocols without compromising either the fidelity or the yield in the 

reaction. The master mix utilizes Phusion Flash II DNA Polymerase, a proofreading DNA 

polymerase modified from Phusion Hot Start II High-Fidelity DNA Polymerase. 

• Extreme speed and high yields 

• 25x higher fidelity than Taq 


252548010 F-548S Phusion® Flash High-Fidelity PCR 

Master Mix 

252548020 F-548L Phusion® Flash High-Fidelity PCR 

Master Mix 

Phusion® Hot Start II 

High-Fidelity DNA Polymerase 

1.7 kb 2.2 kb 2.3 kb (GC-rich) 

M P A B C D M P A B C D M P A B C D M 

P: Phusion® Hot Start II High-Fidelity DNA Polymerase 

A-D: Proofreading DNA polymerases from leading suppliers 


100 x 20µl 

reactions 

500 x 20µl 

reactions 


Finnzymes’ Phusion® Hot Start High-Fidelity DNA Polymerase is the most accurate hot start 

DNA polymerase in the market. It has now been upgraded to allow the use of even wider 

range of primers, including those with low melting temperatures. The new Phusion® Hot 

Start II DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding 

applications such as cloning and high-throughput PCR. Like all Phusion DNA Polymerases, it 

incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA 

polymerase extremely robust, rapid, and accurate. 

• No annealing temperature limitations 

• Allows reaction setup at room temperature 

• Zero-time reactivation due to unique hot start technology 

• Fast PCR enzyme with extreme fidelity (over 6 times more accurate than Pfu) 

• High product yields with low enzyme amounts gives excellent cost/reaction ratio 

• Robust enzyme with high tolerance against PCR inhibitors requires minimal optimization 

Phusion Hot Start II DNA Polymerase provides extreme specificity and abundant yields. Five proofreading 

DNA polymerases from major suppliers were used to amplify 1.7-2.3 kb amplicons from human 

genomic DNA. All amplifications were performed in accordance with the manufacturers’ instructions. 

Phusion Hot Start II DNA Polymerase provided high yields of specific products whereas all other enzymes 

delivered zero or low yields, with some also amplifying non-specific products. 


252549010 F-549S Phusion® Hot Start II High-Fidelity DNA 

Polymerase 

100U (2U/µl) 

252549020 F-549L Phusion® Hot Start II High-Fidelity DNA 

Polymerase 

252540010 F-540S Phusion® Hot Start High-Fidelity DNA 

Polymerase 

252540020 F-540L Phusion® Hot Start High-Fidelity DNA 

Polymerase 

500U (2U/µl) 

100U (2U/µl) 

500U (2U/µl) 


12 

Genomics 

DyNAzyme I DNA Polymerase 

DyNAzyme II DNA Polymerase 


Native thermostable DyNAzyme I DNA Polymerase is isolated and purified from Thermus 

brockianus, which is a Finnzymes proprietary strain. DyNAzyme I DNA Polymerase has a halflife 

of 3 hours at 96 °C and it also contains 5’-->3’ exconuclease activity. DyNAzyme I DNA 

Polymerase is purified free of contaminating endo- and exonucleases. 

• Better thermal stability than that of Taq DNA polymerase 

• Tolerates DMSO better than Taq DNA polymerase 

• High purity, no contaminating activities 

• Supplied with optimized buffers or with Mg2+ free buffers 

• High batch-to-batch consistency 

• Easy to use and optimize 

• Detergent-free buffer also available 


252500010 F-500S DyNAzyme I DNA Polymerase with 

optimized buffer 

100U 

252500020 F-500L DyNAzyme I DNA Polymerase with 


252502010 F-502S DyNAzyme I DNA Polymerase with 

Mg 2+ free buffer and MgCl 2 -solution 

252502020 F-502L DyNAzyme I DNA Polymerase with 

Mg 2+ free buffer and MgCl 2 -solution 

500U 

100U 

500U 

252550010 F-550S DyNAzyme I DNA Polymerase Kit 100 x 50µl 

reactions 

252550020 F-550L DyNAzyme I DNA Polymerase Kit 500 x 50µl 

reactions 


Thermostable DyNAzyme II DNA Polymerase is isolated and purified from an E. coli 

strain carrying a plasmid with the cloned DyNAzyme DNA Polymerase gene from Thermus 

brockianus, which is a Finnzymes proprietary strain. DyNAzyme II DNA Polymerase has a 

half life of 2.5 hours at 96 °C. It lacks 3´--> 5´proofreading activity but does contain 5´--> 3´ 

exonuclease activity. DyNAzyme II DNA Polymerase is purified free of contaminating endoand 

exonucleases. 

Also available in HotStart format with: 

• Increased specificity 

• Prevents the formation of primer-dimers 

• Reaction setup can be done at room temperature 


252501010 F-501S DyNAzyme II DNA Polymerase with 


250U 

252501020 F-501L DyNAzyme II DNA Polymerase with 


252503010 F-503S DyNAzyme II DNA Polymerase with 

Mg2+-free buffer and MgCl2-solution 

252503020 F-503L DyNAzyme II DNA Polymerase with 

Mg2+-free buffer and MgCl2-solution 

1000U 

250U 

1000U 

252508010 F-508S DyNAzyme II PCR master mix 200 x 50µl 

reactions 

252508020 F-508L DyNAzyme II PCR master mix 1 000 x 50µl 

reactions 

252551010 F-551S DyNAzyme II DNA Polymerase Kit 100 x 50µl 

reactions 

252551020 F-551L DyNAzyme II DNA Polymerase Kit 500 x 50µl 

reactions 

252504010 F-504S DyNAzyme II Hot Start DNA Polymerase 

252504020 F-504L DyNAzyme II Hot Start DNA Polymerase 

FRA 

476 

FRA 

527 

250U 

1000U 

T 22 95 59 59 F 22 95 59 40 

DyNAzyme EXT DNA 

Polymerase 


DyNAzyme EXT DNA Polymerase is a versatile and easy-to-use enzyme especially suited 

for handling demanding reaction conditions without time-consuming optimization. It is an 

optimized mixture of DyNAzyme II DNA Polymerase and a proofreading enzyme. DyNAzyme 

EXT DNA Polymerase offers exceptional performance that Taq-based mixtures cannot match. 

• Ideal for difficult templates, such as GC- rich or looped sequences 

• Improved fidelity compared to standard PCR enzymes 

• Generates fragments up to 40 kb in long PCR reactions 

• High yields, robust reactions with standard templates 

• More thermostable than Taq 


252505010 F-505S DyNAzyme EXT DNA Polymerase 250U 

252505020 F-505L DyNAzyme EXT DNA Polymerase 1000U 

illustra Ready-To-Go RT-PCR 

Beads 

illustra RT-PCR is commonly used to amplify cDNA by combining first-strand cDNA synthesis 

of an RNA template with PCR amplification. Typically the reaction is performed in two steps. 

A first-strand cDNA reaction is performed, the resulting cDNA is then transferred to another 

tube containing Taq DNA polymerase and PCR buffer where the reaction mixture is subjected 

to multiple cycles of denaturation, annealing and elongation, resulting in the exponential 

amplification of the target cDNA. Ready-To-Go RT-PCR Beads simplify this process to a 

single-tube, single-reaction procedure. ’ 

Ready-To-Go RT-PCR Beads have been optimized for RT-PCR. Each room-temperature-stable 

bead contains M-MuLV Reverse Transcriptase, RNase Inhibitor, Buffer, Nucleotides and 

Taq DNA polymerase. The only additional reagents required are water, template RNA, and 

primers. 

The Ready-To-Go Bead format significantly reduces the number of pipetting steps, thereby 

increasing reproducibility of the RT-PCR technique and minimizing risk of contamination and 

RNA degradation. Ready-To-Go RT-PCR Beads are provided in either thin walled 0.5 ml or 

0.2 ml tubes compatible with most thermocyclers. Each package of Ready-To-Go RT-PCR 

Beads contains: RT-PCR beads, control reactions and pd(N)6 and oligo(dT) cDNA primers. 


230008250 27-9266-01 illustra Ready-To-Go RT-PCR Beads (0.5 

ml tubes) 


ml tubes) 


ml hinged tube with cap) 

Deoxynucleotide Tripohosphate 

(dNTP) mix 


257107600 R0141 dATP, 100mM Solution 

257107610 R0151 dCTP, 100mM Solution 

257107620 R0161 dGTP, 100mM Solution 

257107630 R0171 dTTP, 100mM Solution 

257107590 R0133 dUTP, 100mM Solution 

257108290 R1191 dITP, 100mM Solution 

257107700 R0241 dNTP Mix 2mM 1 ml 

257107710 R0242 dNTP Mix 2mM 5X1 ml 

257107680 R0192 dNTP Mix 10mM 1 ml 

257107690 R0193 dNTP Mix 10mM 5x1 ml 

100 

reactions 

96 

reactions 

96 

reactions 


In a hurry? Use Direct PCR from Finnzymes 

Finnzymes’ novel approach, Direct PCR, saves you time and cost by allowing amplification 

of DNA directly from the source material. Direct PCR is based on Finnzymes’ Phusion® 

High-Fidelity DNA Polymerases and Phire® Hot Start II DNA Polymerase. These unique fusion 

enzymes have been engineered to incorporate a double-stranded DNA binding domain that gives 

them extraordinary properties. Phusion and Phire DNA Polymerases are exceptionally tolerant 

of many PCR inhibitors present in various sample material. This allows robust amplification in 

conditions where conventional DNA polymerases, like Taq or Pfu, are completely inhibited. 

Phire® Animal Tissue 

Direct PCR Kit 

Finnzymes’ Phire® Animal Tissue Direct PCR Kit has been developed for amplification of DNA 

directly from a wide variety of animal tissues including mice, fish, birds and insects. No DNA 

isolation is required prior to PCR. The kit is based on Phire® Hot Start II DNA Polymerase, a 

specially engineered enzyme with a DNA binding domain. 

Two short and simple protocols 

PCR reaction 

~ 5 minutes in 


incubation buffer 

Two alternative protocols suitable for various purposes. In the direct protocol, a small piece of animal tissue is 

placed directly into the PCR reaction. In the dilution protocol, a small amount of animal tissue is first briefly incubated 

in incubation buffer and 1 µl of this buffer is then added into the PCR reaction. The dilution protocol is useful if several 

PCR reactions are performed from the same sample, for example. 

Fast and reliable PCR from various tissue samples from a 

number of species 

M + - M 

Mouse ear tissue 

Direct protocol 

Dilution protocol 

Bear muscle tissue 

Bird feather 

Cow hair 

Fruit fly wing 

Zebrafish fin 

Direct PCR from various animal tissues. Small samples of various animal tissues were placed directly into 50 µl 

PCR reactions. A specific DNA fragment was amplified with control primers included in the kit or, in the case of fruit 

fly and zebrafish, using control primers whose sequences are provided at Finnzymes website. + and - denote control 

reactions with or without purified DNA. 


252140000 F-140 Phire® Animal Tissue Direct PCR Kit 200 x 50µl 

reactions 

Advantages 

• No need for time-consuming and expensive DNA purification steps 

• Tested with a wide variety of sample types 

• Two simple protocols for various applications 

• Extremely short PCR protocol times 

• Modified Phusion Hot Start II High-Fidelity DNA Polymerase guarantees high 

yields of specific product 

Phire® Plant Direct PCR Kit 

Part of Thermo Fisher Scientific Part of Thermo Fisher Scientific 

Finnzymes’ Phire® Plant Direct PCR Kit is designed to amplify DNA directly from plant 

samples. No DNA isolation is required prior to the PCR. The kit is based on Phire® Hot Start 

II DNA Polymerase, a specially engineered enzyme with a unique DNA binding domain. Phire 

DNA Polymerase is extremely robust and tolerant of many PCR inhibitors present in plant 

material. 

Two short protocols for various needs 

1: Arabidopsis, mtDNA (1 kb) 

2: Arabidopsis, gDNA (3.5 kb) 

3: Capsicum, mtDNA (1.4 kb) 

4: Maize, mtDNA (1.4 kb) 


Dilultion buffer 

Easy, fast and reliable PCR 

A) Leaves B) Seeds 




Two alternative protocols for different purposes. In the direct protocol, a small piece of plant material is placed 

directly in the PCR reaction mix. In the dilution protocol, a small volume of the Dilution Buffer containing crushed plant 

material is added in the PCR reaction mix. The dilution protocol is used for long or difficult amplicons, and for assays 

where several DNA fragments are amplified from the same sample. 

M 1 2 3 4 - + + M M Tomato Maize Apple - + M 

mtDNA (~ 1.4 kb, depending on 

the species) 

Phire Plant Direct PCR Kit allows efficient PCR from various plant leaves and seeds. Small 

samples from different leaves (A) or seeds (B) were placed into 50 or 20 µl PCR reactions, respectively. 

Various amplicons from mitochondrial or genomic DNA were successfully amplified with the Phire 

Plant Direct PCR Kit. Piko Thermal Cycler and UTW reaction vessels were used in PCR. + and - 

denote control reactions with or without purified DNA. 


252130000 F-130 Phire® Plant Direct PCR Kit 200 x 50µl 

reactions 


14 

Genomics 

Phusion® Blood Direct PCR Kit 

Phusion® Human Specimen 

Direct PCR Kit 


Finnzymes’ Phusion® Blood Direct PCR Kit is designed for amplification of DNA from whole 

blood. It eliminates the need for a separate DNA purification step prior to PCR. The kit utilizes 

a modified Phusion® Hot Start II High-Fidelity DNA Polymerase. This highly processive and 

robust PCR enzyme is resistant to the inhibitors present in blood and retains its activity even 

at 40 % blood concentration. Phusion Blood Direct PCR Kit allows analysis of blood samples 

with different anticoagulants, storage conditions and from various species. Tolerates heparin, 

citrate, and EDTA, and is compatible with frozen blood or blood stored on various types of 

filter papers. 


Choose direct or dilution protocol based on your needs 

• buccal swab 

• amniotic fluid 

• skin biopsy 

• hair 

• nail 

• saliva 

Phusion ® Blood Direct 

Blood % 

1 5 10 20 30 40 - + 

40 min 


Dilultion buffer 

A few minutes in incubation buffer 



Short protocols, superior performance 

M + - 1 2 3 4 5 6 M 

Supplier B 

Blood % 

1 5 10 20 30 40 

106 min 


1. Buccal swab (0.5 mm puch) 

2. Hair (1 mm) 

3. Tooth (1 mm piece) 

4. Nail (1x2 mm) 

5. Saliva (0.5 µl) 

6. Amniotic fluid (1.0 µl) 

- + 

Phusion Blood Direct PCR Kit delivers superior performance even at very high blood concentrations. Phusion 

Blood Direct PCR Kit was compared to another kit designed for PCR directly from blood. A 588 bp genomic DNA fragment 

was amplified in the presence of increasing blood concentration in the reaction mixture. PCR was performed according to 

suppliers’ instructions using Finnzymes’ Piko ® Thermal Cycler and ultra-thin walled UTW ® reaction vessels. Total cycling 

times indicated at bottom. + and - denote control reactions with or without purified DNA. 


252547010 F-147S Phusion® Blood Direct PCR Kit 100 x 50µl rxns 

252547020 F-147L Phusion® Blood Direct PCR Kit 500 x 50µl rxns 

Finnzymes’ Phusion® Human Specimen Direct PCR Kit delivers unprecedented convenience 

for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount 

of sample is used directly in the PCR reaction with no prior purification steps. This allows 

significant savings in both time and cost. 

Suitable sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, 

and skin biopsies. The Phusion Human Specimen Direct PCR Kit kit is compatible with fresh, 

frozen, and even formalin-fixed, paraffin embedded human tissue samples. 

Consistent results from various human samples with Direct PCR. Different samples were placed directly into 

20 µl PCR reactions. A 237 bp DNA fragment was successfully amplified using the control primers included in the kit. 

Reactions were run in UTW reaction vessels using Piko Thermal Cycler. + and – denote control reactions with and 

without purified DNA. 


252150000 F-150 Phusion® Human Specimen Direct PCR Kit 200 x 20µl rxns 

T 22 95 59 59 F 22 95 59 40 

Phusion® Site-Directed 

Mutagenesis Kit 


252541000 F-541 Phusion® Site-Directed 

Mutagenesis Kit 


A fast and reliable system for generating mutations 

The Phusion® Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing 

point mutations, insertions or deletions in any type of plasmid DNA. With this kit, the entire 

plasmid is amplified using phosphorylated primers that introduce the desired changes. The 

amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute 

ligation reaction with Quick T4 DNA Ligase. The resulting plasmid can be then transformed 

into any competent E. coli cells. 

• Robust and reliable exponential amplification method 

• No requirements such as special vectors, restriction sites or methylation status for the 

target plasmid 

• No need to destroy the starting template in a separate step 

• Due to the high fidelity of Phusion® Hot Start II DNA Polymerase unwanted secondary 

mutations are minimized 

• Amplification of large plasmids up to 10 kb 

• The hot start modification in the polymerase prevents the amplification of non-specific 

products and unwanted degradation of primers prior to first cycle of PCR 

• Quick T4 DNA Ligase included in the kit; no purification steps before or after the ligation 

• Compatible with all strains of competent E. coli cells 

5’P 

X 

The Phusion ® Site-Directed Mutagenesis protocol 

Point mutation Deletion Insertion option 1 

R 

5’P 

Mut 

X 

5’P 

Target 

plasmid 

X 

F 

5’P 

R 

Del 

5’P 

5’P 

X 

5’P 

Target 

plasmid 

F 

R 

5’P 

Mut 

X 

5’P 

X 

F 

Mutated target 

plasmid 

5’P 

R 

Ins 

5’P 

5’P 

Target 

plasmid 

F 

R 

Del 

5’P 

5’P 

F 

R 

Step 1. 

Amplification of target 

plasmid with two 

phosphorylated primers. 

Step 2. 

Plasmid circularization by 

quick ligation. 

Step 3. 

Transformation into 

E. coli. 

Insertion option 2 

5’P Ins 

5’P 

Target 

plasmid 

20 reactions inc. 10 control 

reactions 

F 

5’P 

R 

Ins 

5’P 

F

Thermo-Start 

Taq DNA polymerase 

For Hot Start and quantitative PCR. 

• Chemically modified thermostable DNA polymerase 

• Eliminates non-specific priming 

• Reactions can be prepared and held at room temperature 

• Contains optimised buffer for improved yields 

Inactive until ‘switched on’ by high temperature incubation. Incremental activation of enzyme 

increases reaction specificity. 

Concentration 5U/µL 

Recombinant Yes 

5’ to 3’ exonuclease (strand displacement) Yes 

activity 

3’ to 5’ exonuclease activity No 

3’ adenylation activity Yes 

Free of nucleases Yes 


295203965 AB-1908/A Thermo-Start® DNA polymerase plus 10X 

Thermo-Start® buffer containing 15mM 

MgCl2 250 units 

295203895 AB-0908/A Thermo-Start® DNA polymerase plus 10X 

Thermo-Start® buffer and 25mM MgCl , 2 

20mM dNTPs 

250 units 

295203910 AB-1057/A Thermo-Start® DNA polymerase plus 10x 

High performance buffer, 25mM MgCl 2 

Thermoprime Plus 


For PCR of genomic, viral and plasmid templates. 

• Enhanced thermal stability at DNA denaturation temperatures 

• Extended long term stability 

• Shipped at ambient temperature with no loss of activity 

• Proven performance in excess of 12kb for basic templates 

Thermoprime Plus DNA Polymerase is a 94kDa ultra-pure recombinant thermostable DNA 

polymerase obtained by high level expression of the Taq DNA polymerase gene in Escherichia 

coli. 




activity 




250 units 


295203935 AB-1301/A Thermoprime Plus with 10X buffer 

containing 15mM MgCl2 250 units 

295203800 AB-0301/A Thermoprime Plus with 10X buffer, 

separate vial of 25mM MgCl2 250 units 

295203885 AB-0790/A Thermoprime Plus with 10X ReddyMix 

buffer, separate vial of 25mM MgCl2 250 units 

295209740 AB-1605/A Simple red DNA polymerase 250 units 

295209745 AB 1605/B Simple red DNA polymerase 2,500 units 


Standard PCR applications. 

A 94kDa ultra-pure recombinant thermostable DNA polymerase obtained by high level 

expression of the Taq DNA polymerase gene in E. coli. 




activity 





295203920 AB-1192/A Taq DNA polymerase with 10X buffer 

containing 15mM MgCl2 250 units 

295203775 AB-0192/A Taq DNA polymerase with 10X buffer, 

separate vial of 25mM MgCl2 250 units 

295203825 AB-0406/B Red Hot® DNA polymerase with 10X 

buffer, separate vial of 25mM MgCl2 500 units 

2x Reddymix PCR Master Mix 

ThermoPrime Taq DNA Polymerase is an ultra-pure recombinant thermostable 

DNA polymerase obtained by high level expression of the Taq gene 

in Escherichia coli. With enhanced thermal stability, non-specific binding 

of template and primers is minimized leading to a decrease in the amount 

of non-specific product. Being a recombinant enzyme, it is characterized by 

significantly higher purity than those enzymes that are native. This higher 

level of purity gives superior lot-to-lot reproducibility. ThermoPrime has 5’ 

to 3’ Taq DNA polymerase activity as well as 5’ to 3’ strand displacement (exonuclease) 

activity. The convenient Master Mix format contains all the components required for PCR (with 

the exception of template and primers), reducing protocol steps and minimizing the risk of 

pipetting errors and sample contamination 


295204060 AB-0575/DC/LD/ 2 x ReddyMixPCR master mix 


16 

Genomics 

DreamTaq Green DNA 

Polymerase 

• High yields of PCR products without optimization, MgCl2 included into the buffer. 

• Higher sensitivity compared to conventional Taq DNA polymerase. 

• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral 

DNA. 

• Robust amplification of difficult templates. 


257101340 EP0711 

200 u 

257101350 EP0712 DreamTaq Green DNA Polymerase, 500 u 

5 u/μl supplied with 10X DreamTaq 

257101360 EP0713 Green Buffer 

5x500 u 

257101370 EP0714 20x500 u 

257101300 EP0701 

257101310 EP0702 

DreamTaq DNA Polymerase, 5 u/μl 

500 u 

257101320 EP0703 

supplied with 10X DreamTaq Buffer 

5x500 u 

DreamTaq Green 

PCR Master Mix (2X) 

illustra PuReTaq Ready-To-Go 

PCR Beads 

illustra PuReTaq Ready-To-Go PCR Beads are a newly designed, premixed, predispensed 

reactions designed for performing standard PCR. The single-dose, ambient-temperaturestable 

beads ensure greater reproducibility between reactions, minimize pipetting steps and 

reduce the potential for pipetting errors and contamination. The only additional reagents are 

water, template DNA and primers. The beads are provided in either 0.5 ml or 0.2 ml tubes that 

are compatible with most thermal cyclers. 


230015050 27-9557-01 illustra PuReTaq Ready-To-Go PCR 

Beads (0.2 ml tubes/plate) 


Beads (0.5 ml tubes) 


Beads (0.2 ml hinged tube with cap) 

200 u 

257101330 EP0704 20x500 u 

• Convenient – DreamTaq Green DNA Polymerase in a 

ready-to-use master mix. 

• High yields of PCR products without optimization. 

• Higher sensitivity compared to conventional Taq DNA 

polymerase. 

• Amplification of long targets up to 6 kb from genomic 

DNA and up to 20 kb from viral DNA. 

• Robust amplification of difficult templates. 




257107300 K1081 

DreamTaq Green PCR Master Mix (2X) supplied 

4x1.25 ml 

257107310 K1082 

with Water, nuclease-free 

20x1.25 ml 

257107280 K1071 

DreamTaq PCR Master Mix (2X) 

4x1.25 ml 

257107998 K1072 

supplied with Water, nuclease-free 

20x1.25 ml 

96 

reactions 

100 

reactions 

96 

reactions 

T 22 95 59 59 F 22 95 59 40 

qPCR Reagents 

DyNAmo SYBR Green and Probe 

qPCR Kits 

DyNAmo qPCR Kits are ready-to-use 2x master mix for sensitive, quantitative real-time PCR. 

These master mixes contain a modified Thermus brockianus DNA polymerase and all the other 

reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. 

The modified DNA polymerase in this kit incorporates a non-specific DNA-binding domain that 

lends physical stability to the polymerase-DNA complex. 

• Sensitivity: Optimized 2x master mix allows reproducible detection of low-copy number 

templates. 

• Speed: More efficient amplification coupled with increased polymerase processivity 

results in earlier C(t)s and shorter overall qPCR reaction times. 

• Convenience: 2x master mix, one tube only. ROX passive reference dye is provided in a 

separate vial in some kits. 

• Broad dynamic range: DyNAmo qPCR Kits detect as few as one copy of the target gene 

and achieves a dynamic range of up to eight orders of magnitude. 


252400010 F-400S DyNAmo SYBR® Green 

qPCR Kit 

252400020 F-400L DyNAmo SYBR® Green 


252400030 F-400RS DyNAmo SYBR® Green qPCR 

Kit with ROX 

252400040 F-400RL DyNAmo SYBR® Green qPCR 

Kit with ROX 

DyNAmo HS SYBR® Green 



40 x 50µl 

reactions 

200 x 50µl 

reactions 

40 x 50µl 

reactions 

200 x 50µl 

reactions 

252450010 F-450S DyNAmo Probe qPCR Kit 40 x 50µl 

reactions 

252450010 F-450L DyNAmo Probe qPCR Kit 200 x 50µl 

reactions 


DyNAmo HS SYBR® Green qPCR Kit is a ready-to-use 2x master mix for highly specific 

quantitative PCR. The master mix contains all reagents needed for qPCR. Only template 

DNA and PCR primers need to be added by the user. The 2x master mix includes a hot start 

version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase 

incorporates a non-specific DNA-binding domain that lends physical stability to the 

polymerase-DNA complex. 

• Specificity: DyNAmo HS SYBR Green master mix minimizes the formation of unwanted 

primer-dimers and extension of non-specifically bound primers during reaction setup. 

• Sensitivity: Optimized master mix allows reproducible detection of low-copy number 

templates. 

• Broad dynamic range: DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of 

the target gene and achieves a dynamic range of up to eight orders of magnitude. 

• Convenience: 2x master mix, one tube only. ROX passive reference dye provided in a 

separate vial. 


252410010 F-410S DyNAmo HS SYBR® Green qPCR Kit 40 x 50µl 

reactions 

252410020 F-410L DyNAmo HS SYBR® Green qPCR Kit 200 x 50µl 

reactions

qPCR Reagents 

DyNAmo Flash qPCR Kits 

DyNAmo Flash SYBR® Green qPCR Kit and DyNAmo Flash Probe qPCR Kit offer enhanced 

performance for real-time qPCR. These kits provide sensitive and reproducible detection of 

target DNA with shorter run times in both fast and conventional instruments. 

• Extremely fast protocols: combined annealing and extension step of only 15 s 

• Specific and sensitive detection of a wide range of template concentration 

• Included dUTP allows the use of UNG for prevention of carry-over contamination 

• Convenient 2x master mix and optimized protocols 

• Validated with all major real-time instruments 

• Licenced for qPCR 

• qPCR results faster without compromising performance 

• Reliable and robust amplification 

• High specificity and sensitivity 

• High signal intensity 


252415010 F-415S DyNAmo Flash SYBR® Green qPCR Kit 100 x 20µl rxns 

252415020 F-415L DyNAmo Flash SYBR® Green qPCR Kit 500 x 20µl rxns 

252455010 F-455S DyNAmo Flash Probe qPCR Kit 100 x 20µl rxns 

252455020 F-456S DyNAmo Flash Probe qPCR Kit 500 x 20µl rxns 

Maxima SYBR Green qPCR 

Master Mix (2X), ROX Solution 

provided 



Maxima SYBR Green qPCR Master Mix (2X) is a ready-to-use solution optimized for 

quantitative real-time PCR and two-step real-time RT-PCR on most real-time PCR machines. It 

detects low copy number targets and has a wide linear range. The ROX solution is provided in 

a separate tube for use with machines that requires ROX as a passive reference dye. 


257106980 K0251 Maxima SYBR Green qPCR Master Mix 

(2X), ROX Solution provided 

257106990 K0252 Maxima SYBR Green qPCR Master Mix 

(2X), ROX Solution provided 

Real-time PCR 

FRA 

782 

FRA 

1113 

200 

reactions 

1000 

reactions 

Real-time PCR (also known as qPCR) has quickly become the gold standard 

technique for quantitation of nucleic acids in biological samples. Its 

unsurpassed sensitivity has made it possible to use much smaller sample 

quantities than ever before. The technology is based on the nobel prize 

winning Polymerase Chain Reaction which amplifies a specific DNA fragment 

DyNAmo ColorFlash qPCR Kits 

Finnzymes’ new DyNAmo ColorFlash qPCR Kits are a superior 

choice for fast, quantitative real-time analysis. These kits offer excellent performance in 

detection and quantification of DNA sequences from various sources. DyNAmo ColorFlash 

qPCR Kits are designed to ease qPCR setup and minimize errors by providing a simple way to 

track pipetting of reaction components. 

• Minimized risk of pipetting errors during reaction setup 

• Also beneficial when using white reaction vessels 

• Extremely fast protocols due to combined annealing and extension step of only 15 s 

• Specific and sensitive detection of a wide range of template concentrations 

• dUTP included in the 2x Master Mix allows the use of UNG for prevention of carry-over 

contamination 

• Licensed for qPCR 

+ = 

Master mix Sample in buffer qPCR reaction mix 



252416010 F-416S DyNAmo ColorFlash SYBR® Green qPCR Kit 100 x 20µl 

reactions 

252416020 F-416L DyNAmo ColorFlash SYBR® Green qPCR Kit 500 x 20µl 

reactions 

252456010 F-456S DyNAmo ColorFlash Probe qPCR Kit 100 x 20µl 

reactions 

252456020 F-456L DyNAmo ColorFlash Probe qPCR Kit 500 x 20µl 

reactions 

Maxima Probe qPCR Master Mix 

(2x), ROX solution provided 


Maxima Probe qPCR Master Mix (2x)is a ready-to-use solution optimized for quantitative realtime 

PCR and two-step real-time RT-PCR on most real-time PCR machines. The ROX solution 

is provided in a separate tube for use with machines that requires ROX as a passive reference 

dye. The use of Maxima Probe qPCR Master Mix in real-time PCR ensures reproducible, 

sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. 


257107000 K0261 Maxima Probe qPCR Master Mix (2x), ROX 

solution provided 

257107010 K0262 Maxima Probe qPCR Master Mix (2x), ROX 

solution provided 

200 

reactions 

1000 

reactions 

exponentially to huge amounts that can readily be detected by for example fluorescent 

staining on gels. In real-time PCR, the amount of nucleic acid is monitored during the 

amplification process and one can obtain information about the quantity of a specific gene. 

Different technologies to detect the fluorescence exist, including non-specific dyes such SYBR 

Green or DNA-probes such as TaqMan probes. 


18 

Genomics 

ALPS 50 V microplate heat sealer 

Benefits 

• Ideal solution for PCR and long term plate storage 

• Ergonomic design reduces operator effort 

• Compact footprint and lightweight design 

• Variable time and temperature controls offer 

reproducible sealing results 

• Wide range of heat seals available 

Cat.No Alt.No Decription 

375001443 AB-1443 ALPS 50 V microplate 

heat sealer 

EU 0.2 ml Thin-wall Tube 

Fits almost all Cycler models and qPCR Cycler 

models. (Equal to B79001) 

Cat.No Pkg.Size 

BIO B77301 

BIO B77201 


BIO 157300 

BIO 157200 

1000 - Standard 

1000 - Low profile 

Opti-Seal Optical Disposable 

Adhesive (Also Alu Seal) 

Opti-Seal Provides the best sealing option for EU 

plates. 

100 

100 - Alu Seal pierceable 

384 Well Plate 

This superior full-skirted 384 well EU plate 

is designed to standard 384 cyclers and fully 

optimized for robotic applications 


BIO B70515 40 - ABI, Biorad, Eppendorf, 

Biometra, MJ 


BIO B69901 

BIO B59901 

T 22 95 59 59 F 22 95 59 40 

ALPS 3000 Microplate Heat Sealer 

The Thermo Scientific ALPS 3000 

microplate heat sealer is a fast, 

compact heat sealer created for 

optimal robotic integration in high 

throughput environments, yet allows 

manual, benchtop control. 


375003000 AB-3000 ALPS 3000 Heat 

Sealer 

PCR plastic for all PCR and qPCR Thermo Cyclers - special plates for ABI and Roche 

EU 0.2ml Thin-wall 8-tube 

strip 

Closure can be accomplished with any Bioplastics 

EU 8-cap strip. (Equal to C79601) 

120 - Standard 

120 - Low profile 

96 x 0.2ml Plate 

These EU 96 well regular profile plates are 

non-skirted and designed for qPCR applications. 

(Equal to B70501) 


BIO B50501 25 - Standard plate 

96 x 0.2ml Plate - ABI+Fast 

These EU 96 well plates are a superior quality and 

price alternative designed to fit any Applied Biosystems 

regular of Real-Time PCR thermal cycler as well 

as ABI sequencers. 

549 


BIO AB17500 

BIO AB19800 

25- ABI 

25 - Low profile fast. ABI + Fast 

EU Optical Wide area 8-Cap 

Strip 

For closure of all BIOplastics type (q)PCR tubes, strips 

and plates. (Equal to B79701) 


BIO B57801 120 - For all 8-strip 

96 x 0.2ml Plate 

This EU 96 well low profile plate is non-skirted 

and designed for qPCR applications. (Equal to B60101) 


BIO B50601 25 - Low profile 

96 x 0.2ml Plate - Roche 

These EU 96 well low profile plates are 

semi-skirted and designed for qPCR applications and 

to fit Roche 480 cyclers with 96 well blocks. 

599 


BIO B17489 25 - white. Roche 480 

499

PCR–Products, BRAND 

BRAND has significantly expanded its product range of extra-thin-wall disposable products, 

which were specially developed to satisfy the demands of PCR applications The Polymerase 

Chain Reaction (PCR) revolutionized molecular biology and is used today in many different 

applications. The size of PCR disposables must be as varied as the applications for which they 

are used. 

Quality features 

• Manufactured under the most modern cleanroom conditions. 

• DNase-, DNA- and RNase-free. 

• PP, extra-thin uniform wall thicknesses to provide the optimal thermal transfer and short 

cycle times. 

• Suitable for use in common thermal cyclers. 

• qPCR: Products which are suitable for Real Time PCR (qPCR). 

Single PCR tubes and strips 

PP. PCR tubes with attached caps are compatible with leading thermal cyclers with heated 

lids.The tightly fitting cap reduces evaporation to a minimum and can be opened and closed 

with ease. 

Cat. No. Volume ml Description Color Pkg.Size 

Single PCR tubes with attached cap 

158781300 0,2 domed cap clear 1000 

158781305 0,2 flat cap clear 1000 

158781310 0,5 flat cap clear 1000 

Strips of 8 PCR tubes with attached cap strips 

158781330 0,2 attached cap strips clear 125 strips 

Strips of 8 PCR tubes with attached flat individual covers 

158781332 0,2 Standard, qPCR clear 120 strips 

158781333 0,15 Low Profile, qPCR clear 120 strips 

Strips of 8 PCR tubes with detached cap strips 

158781320 0,2 no cap strips clear 125 strips 

158781340 strips of 8 caps domed clear 125 strips 

158781334 strips of 8 caps, qPCR Flat clear 125 strips 

Strips of 12 PCR tubes with detached cap strips 

158781280 0,2 ml no cap strips clear 125 strips 

158781290 strips of 12 caps domed clear 125 strips 

24-well and 48-well qPCR Plates 

PP. 0.2 ml wells. These plates reduce material 

costs and allow work on a compact PCR 

plate even with small sample throughputs. 

Alphanumeric coding embossed and stamped 

in blue. 

Cat. No. Number of wells Description Pkg.Size 

158781411 24 no skirt 40 

158781415 48 no skirt 20 

96-well qPCR Plates 

PP. 0.2 ml wells. 96-well plates are standard for 

PCR applications. Different variants facilitate the 

optimal use in the most common thermal cyclers. 

Cat. No. Description pack of 

158781350 No skirt * 50 

158781352 Elevated skirt 50 

158781353 Full skirt 50 

158781400 Half skirt * 50 

* alphanumeric coding embossed and stamped in blue 

384-well PCR Plates 

The 40 μl wells can hold sample volumes of 2 

μl to 30 μl. This helps to reduce reagents costs 

and shortens cycle times. The plates can be filled 

using multichannel pipettes or robotic systems. 


158781345 Full skirt, PP 50 

158781347 Full skirt, PP, for qPCR 50 

158781348 Full skirt, rigid plate 50 

PCR Sealing Mats 

PCR sealing mats have been designed to 

fit BRAND PCR plates exactly and reduce 

evaporation losses by up to 75% compared to 

conventional systems. They are characterized by 

their high flexibility, tight sealing, autoclavability 

and are easily pierced by pipette tips. 

Cat. No. Product Material 

158781402 24-well mat (for Cat. No. 158-781411) TPE 

158781403 48-well mat (for Cat. No. 158-781415) TPE 

158781405 96-well mat (for Cat. No. 158-781350, 158-781400) TPE 

158781407 96-well mat (for Cat. No. 158-781352, 158-781353) Silicone 

158781408 384-well mat (for Cat. No. 158-781345, 158-781347) Silicone 

Sample Preparation Quantitation & QC cDNA synthesis PCR & qPCR Reagents PCR Plastics Thermocyclers Electrophoresis & analysis 


20 

Genomics 

White PCR Products for qPCR 

The use of Real-Time PCR (qPCR) is becoming increasingly widespread. In particular, the direct 

quantification of the DNA formed can be achieved by the use of fluorescence measurement. 

The new line of white PCR products by BRAND offers significantly better results in this 

application than transparent 8-tube strips or plates. The different products in this line are 

uniformly colored with TiO2 (titanium dioxide), so that in combination with the smooth 

surfaces, the optimum reflection of the fluorescence signal is provided. 

Strips of 8 PCR tubes 

PP, white for qPCR. PP. 8 connected 0.2 ml 

tubes. Separate, domed or flat caps are 

available in strips of 8. They are easy to open 

and close without tools. The tubes have a 

holding strap at one end, and the cover strips 

on each cap have a small lip on the side for 

careful, contaminationfree opening. Pack of 

125 Strips. 

Cat. No. Description 

158781325 Strips of 8 PCR Tubes 

158781334 Strips of 8 PCR Lid (flat) 

24- and 48-well PCR-Plates 

PP, white. For qPCR. 0.2 ml wells. 

Cat. No. Number of wells Description pack of 

158781412 24 no skirt 40 

158781416 48 no skirt 20 

96-well PCR-Plates 



158781354 no skirt 50 

158781357 half skirt 50 

384-well PCR-Plates 



158781358 full skirt 50 

Sealing Films, Self-adhesive 

In case the PCR plates need not only to be covered, but also securely sealed, self-adhesive 

sealing films are available. These film sheets can be easily applied on the plates and removed 

also without the use of expensive equipment. 

Cat. No. Application Description Material 

158781391 Real Time 

PCR 

single film, hightransparency 

Temperature 

range 

Polyester -40 to +120 

°C 

pack of 

T 22 95 59 59 F 22 95 59 40 

100 

PCR tubes and strips 

• Flat or domed caps 

• Thin wall design for maximum PCR* 

efficiency 

• Certified free of DNase/RNase 


210040937 FB69875 Tubes, flat capped, 0,2 ml 1,000 

210040934 FB69855 Tubes, dome capped, 0,2ml 1,000 

210040936 FB69865 8 tube strip, flat capped, 0,25ml 250 

210040935 FB69860 8 tube strip, dome capped, 0,25ml 250 

PCR microplates 

• Suitablefor0.2mLthermalcyclerblocks 

• Maximumtubecapacity0.25mLwhen 

capped 

• Can be cut into individual tubes 

• Ideal for use in 96 well V bottom 

thermal cyclers 


210040836 FB69882 Microplate for PCR, 96 well, no skirt 25 

210040840 FB69894 Microplate for PCR, 96 well, semi-skirt 25 

210040914 FB69887 Microplate for PCR, 96 well, skirted 25 

PCR cooler from Eppendorf 

By changing color from violet to pink or from 

dark blue to light blue, the PCR Cooler shows 

when upper temperature value of 7°C has 

been exceeded. Decisive for the cooling of 

samples is thereby the color value in the 

depressions of the PCR Cooler. The individual 

temperature of single tubes can be determined 

from the coloring of the respective receptacle 

bore. 

• Cools sensitive samples reliably for more 

than an hour at 0°C (with 2 hour precooling at -20°) 

• Temperature safety through color change when temperature is exceeded: > 7 °C : pink or 

light blue < 7 °C : violet or dark blue 

• Simple, space saving 

• Instead of ice bath: safe from contamination 

• No freezing of samples with special cone contour 

• Each individual bore shows the temperature of each single tube 

• In the 96 - well format 

• For 0.2 ml and 0.5 ml, tube strips, plates and single tubes 

Cat.No Alt.no Description 

275106810 3881000015 0.2mL starter set (1x pink, 1x blue) 

275105090 3881000023 0.2mL, pink 

275106510 3881000031 0,2ml, blue

KingFisher Flex - Thermo Scientific 

Thermo Scientific KingFisher Flex uses patented magnetic separation technology for a rapid 

sample preparation process of nucleic acids, proteins and cells. The new KingFisher Flex 

offers an expanded sample volume range, which in turn increases the yield by five times. With 

minimum hands-on time, KingFisher Flex offers the highest purification capacity with excellent 

reproducibility and high quality. 

Unique technology 

Thanks to the innovative idea of transferring magnetic particles instead of liquids, KingFisher 

Flex offers a rapid and reproducible purification of high-quality DNA, RNA, proteins and cells 

from various and often difficult starting materials like blood, cell cultures, tissue lysates, 

soil and faeces. The technology is based on magnetic rods which move particles through 

the various purification phases – binding, mixing, washing and elution. Automation and 

elimination of liquid handling result in a reliable and robust purification process. 

Increased flexibility 

KingFisher Flex is a truly flexible solution for different types of sample processing needs. With 

the 24-rod magnet head and 24-well deep well plate, customers can raise the processing 

volume up to 5 ml resulting in increased yield of the purified product. For higher throughput 

needs, 96 samples can be processed in different working volumes (20-1000 μl) using 96-rod 

magnet head and appropriate 96-well plates. 

New BindIt Software 

With the new BindItTM Software, customers can easily design new or modify existing 

purification protocols for their own applications. Furthermore, BindIt Software enables setting 

of user access control levels, clear reporting and advanced protocol management. Readymade 

and validated purification protocols for different types of applications are available, offering 

customers the possibility to choose the best possible method for a sample process. BindIt 

Software is compatible with KingFisher Flex but also with other KingFisher family instruments. 

Purification protocols can be run either via PC or using KingFisher as a stand-alone instrument. 

Highest throughput on the market 

KingFisher Flex achieves the highest throughput on the market – up to 96 samples can be 

processed even under 15 minutes. BindIt Software enables KingFisher Flex to interface with 

liquid handling, robotics and plate stacking instruments, providing a fully automated solution 

and the highest throughput for all research, biotech, pharmaceutical and quality control 

laboratories. 

Cat.no Alt.No Description 

238-102110 5400610 KingFisher Flex 

Nucleic acid purification 

instrument, iPrep 

For DNA and RNA purification. 

iPrep purification system 

The iPrep purification instrument takes advantage of ChargeSwitch technology, a major 

advance in DNA purification. 

Unlike silica-based purification methods, ChargeSwitch® technology uses 100% aqueous buffer 

systems, and avoids hazardous reagents that can inhibit downstream applications. Additionally, 

it excels at extracting DNA from forensic samples and buccal scrapes. 

Advantages: 

• Flexibility to process up to 12 samples per run plus a control 

• Fast turnaround time - 18 minutes per run 

• Prepackaged reagent cartridges that eliminates set-up variation 

• Ease of use – true push-button protocol 

• Small footprint- uses only 500mm of bench space 

• Integrated ChargeSwitch technology for inhibitor-free purification 

Cat.No Description 

307102338 iPrep purification instrument 

307106455 iPrep Purelink total RNA kit 

307106342 iPrep Purelink gDNA blood kit 

307106457 iPrep Trizol Plus RNA kit 

Applications for KingFisher Flex: 

• Clinical and biomedical research 

• Pharmacogenomics 

• Applied testing 

• Forensics 

• Biomarker discovery 

• Veterinary diagnostics 

• Quality control of food, beverages and water 

• Plant and seed quality testing 

• Protein research 


22 

Genomics 

Quantitation and QC 

Meet your next labpartner: 

Nanodrop 

As the industry leader in micro-sample quantitation, Thermo Scientific NanoDrop products 

meets the needs of today’s laboratory scientist - with instruments that are innovative, simple 

and robust. 

NanoDrop 2000 - the classic 

• Easy to use, simply pipette sample direct 

onto the pedestal 

• Micro-volume sample size (0.5µl-2.0 µl) 

• No dilutions, even for highly concentrated 

samples (up to 15,000 ng/ µl for dsDNA) 

• Fast, less than five second measurement time 

• User-friendly software ---with features such 

as custom methods 

FRA 

74.900 

NanoDrop 8000 - for 8 samples at a time 

• Measure nucleic acid and protein concentration of samples as small as 1 µL 

• Single sample mode or up to eight samples at a time 

• Analysis of 96 samples in less than 6 minutes 

• Sample Position Illuminator which increases efficiency and reduces error by keeping 

track of the samples to be measured 

• Perfect for core labs, bioprocessing QC/manufacturing environments, biorepositories and 

molecular diagnostics labs. 

Quantitation and quality control 

After extraction of RNA and DNA from samples such as tissues or cells, it is 

important to have an idea of the quantity, quality and purity of the extracted 

material. It can even be a good idea to input the same amount of RNA of each 

sample into each cDNA synthesis reaction to minimize variation between 

samples. UV-spectroscopic methods, such as the NanoDrop, use the absorbance 

T 22 95 59 59 F 22 95 59 40 

NanoDrop 2000c - with cuvette reader 

• Dual modes---choose the measuring option right for your sample ---pedestal or cuvette 

• Very broad concentration range for measuring very low concentrations or very high 

concentrations 

• Cuvette capability for kinetics studies and cell culture (OD 600) measurements 

• Small footprint, perfect for any bench 

NanoDrop 3300 - for fluorescence 

• Sample size as small as 1 µl 

• Mass detection limit more than one order 

of magnitude lower than conventional 

fluorometers 

• UV, Blue and White LED excitation, 

covering a broad wavelength range 

• No filter changes, no monochromator 

• Ability to analyze multiple emission 

profiles from a single sample 

• Simplicity of use – just pipette and wipe 

Cat.No Description 

370812000 NanoDrop 2000 Spectrophotometer 

370802000 NanoDrop 2000c Spectrophotometer with cuvette capability 

370808000 NanoDrop 8000 Multi-sample Spectrophotometer 

370883300 NanoDrop 3300 Fluorospectrometer 

at 260nm to quantify the nucleic acids. Note though that absorbance at 260nm does not 

discriminate between RNA and DNA and also does not discriminate between intact nucleic 

acids and free nucleotides. One can also use the ratio of absorbances at 260/280 to assess 

the amount of contaminating protein and/or phenol. UV-spectroscopic methods are widely 

used because of their simplicity and that no additional reagents are required. The NanoDrop 

also only utilizes 1-2µl of the sample. Fluorometric methods, such as the Qubit, can also 

be used and different fluorescent dyes will discriminate between DNA and RNA. Also 

fluorometric methods tend to be more sensitive.

Gel Electrophoresis and Analysis 

Horizontal 

Electrophoresis Gel Systems 

Injection moulded gel tanks 

Gel dimensions, 

(W x L) 

Unit dimensions, 

(W x L x H) 

Max. sample 

capacity 

SUB-GEL Mini 

• Suppliedwith7x7cmand 

7 x 10cm gel trays 

• Economic low gel and 

buffer volumes 

• Small footprint 

Technical Specifications 

7 x 7cm 

7 x 10cm 

21 x 9 x 9cm 

Buffer volume 225mL 

Combs available: 

No. of samples 

Thicknesses 

7 x 7cm tray – 

32 samples 

7 x 10cm tray – 

64 samples 

1, 2, 4, 8MC, 8, 

10, 12MC, 16 

0.75, 1, 1.5, 2mm 

Catalogue No Description 

210079000 SUB-GEL Mini, 7cm x 7cm & 7cm x 10cm UV Tray, 2 x 8 sample combs 

(1mm), loading guides and dams 

HE 33 mini submarine 

Electrophoresis unit 

The HE 33 mini submarine is 

available as a complete version 

for casting and running 7 x 10 

cm gels and a comb. 


287000760 HE33815 HE 33 Mini Submarine Complete 

package 

HiYield Gel/PCR DNA Extraction Kit 

Designed to recover or concentrate DNA fragments (50bp- 

10Kb) from agarose gels, PCR or other enzymatic reactions. 

The unique dual purpose application and high yield DNA/ 

minicolumn make this kit exceptional value. The method uses a 

chaotropic salt, guanidine thiocyanante to dissolve agarose gel 

and denature enzymes. 

Cat.No Description Pkg.Size 

288000560 HiYield Gel/PCR DNA Extraction Kit 100 preps 

Power Supply 

The Hoefer PS200-HC/PS300B are 

designed for all electrophoresis and semidry 

lotting applications. It can be operated 

in constant current or constant voltage 

mode. The small footprint and large handle 

make the Hoefer PS200-HC/PS300B easy 

to move around and use in your lab. The 

large LED is easy to read. Just set the 

constant parameter, set the timer, if 

desired, and push Start. 

The Hoefer PS200-HC/PS300B are 

designed and certified in accordance with 

the applicable UL, EN and CSA electrical 

standards. For safety reasons, 4mm 

plugs with fixed insulating sheaths are 

recommended, standard on most Hoefer 

equipment. There are no adapters required 

when used with Hoefer equipment. 


287001321 PS200-HC 200 V, 2 A, 200 W maximum power supply with universal 

input voltage and 4 pairs of 4-mm output jacks. 

287001326 PS300B 300V, 500mA, 90 W maximum power supply with universal 

input voltage and 4 pairs of 4-mm output jacks. 

BlueMarine 200 

BlueMarine 200 has the gel formats 15 x 15 cm or 15 x 20 cm, for best resolution or high 

throughput analysis. The pacage contains the main unit and also: 1 removable UV transparent 

gel tray (15 x 20 cm), 1 removable UV transparent gel tray (15 x 15 cm), 2 gel casting gates, 2 

combs (1.0 mm, 16 samples). 

Operational Data (Tray: 15 x 15 cm / 15 x 20 cm): 

Maximum operating voltage: 500 V / 500 V 

Maximum operating current: 300 mA / 300 mA 

Approx. gel volume (5 mm gel): 115 ml / 150 ml 

Possible comb positions: 2 / 4 

Maximum of loadable samples: 62 / 124 

Electrode separation: 28.5 cm / 28.5 cm 

Recommended volts per cm: 20 - 200 / 20 - 200 


18742000 55015301 BlueMarine 200 


24 

Genomics 

DNA marker, 100bp PCR DNA ladder 

Ideal for size assessment and quantitative determination of DNA Mass, blunt ended DNA. 


167924188 BPE2571-100 PCR size confirmation 

100bp PCR DNA ladder (25 to 1,000bp), 

12 bands 

1mL 

Ethidium bromide 

Ethidium bromide is commonly used to stain electrophoresis gels. The compound forms a 

fluorscen complexes with nucleic acids and these can be viewed unde UV light. 


164256530 802511 Ethidium bromide in water solution 

10mg/ml 

10 ml 

Agarose 


257107860 R0491 TopVision Agarose 100g 

257107870 R0492 TopVision Agarose 500g 

Agarose Gel loading dye,6x 

May be added to DNA and RNA samples prior to electrophoresis on agarose gels. 


167916302 BPE633-5 Agarose Gel loding dye 6x 5mL 

Decontaminant wipes, RNase AWAY, 

DNA AWAY 

• Easily removes RNase, DNase and DNA 

contaminants from benchtops, instruments, 

pipettors, glass and plasticware 

• Ideal for labware and surfaces that cannot be 

autoclaved 

• Leaves no residue on work surfaces when 

used as directed 



229007006 7006 RNase AWAY canister 25 

229007007 7007 RNase AWAY, individually wrapped 

towelettes 

35 

229007008 7008 DNA AWAY®, canister 25 

229007009 7009 DNA AWAY®, individually wrapped 

towelettes 

35 

T 22 95 59 59 F 22 95 59 40 

GeneRuler DNA Ladders 

(10 bp – 48502 bp) 

GeneRuler line offers the widest range of DNA ladders, 

including the popular 1 kb and 100 bp ladders. All our DNA 

and RNA ladders are assembled of chromatography purified 

individual DNA fragments and form exceptionally clear sharp 

bands on gels. 

Features: 

• Ideal for both DNA sizing and approximate quantification. 

• Sharp bands. 

• Bright reference bands (given in red). 

• Supplied with loading dye for sample DNA. 

New Gel documentation 

Gel Logic 212 PRO 

• HIGH PERFORMANCE 

• HIGH RESOLUTION 

• LOW COST 

The new Gel Logic 212 PRO is a fully 

automated system that offers ease 

of use, sophistication, versatility 

and performance not typically seen 

on imaging systems aimed at the 

basic research community. The 

GL 212 PRO is ideal for imaging 

fluorescence or colorimetric data in 

sample formats such as gels (DNA/ 

Coomassie/Silver stain) membranes, 

and 96 well plates. Features include 

a 1.4megapixel grade 0 (0 defects) 

CCD, motorized 8-48mm6x zoomlens, 

true 12-bit data capture, virtual 

illumination controls, a rollout transilluminator, 

and newly designed 

white light table (optional). 

The Carestream Molecular Imaging 

software (included with the system) now features a variety of new, easy-to-use functionalities 

such as true autofocusing and directed autoexposure that allow the user to capture perfectly 

exposed images in just a few clicks. for all yourmolecular imaging needs High resolution and 

innovative, automated controls make it easy to capture perfectly exposed images every time. 

• 1.4 megapixel scientific grade camera 

• True 12-bit data capture 

• Auto-focus and easy illumination 

• Motorized 8-48mm lens with 6x zoom 

• Unbeatable price/performance 

Art.nr Benämning 

183415884 GL212pro Gel-doc instrument 


FRA 

69.500

Vi har også en helt ny brosjyre med Fermentas og Finnzymes. 

Den finner du på www.fishersci.no 

E fisher.no@thermofisher.com W www.fishersci.no

26 

Genomics 

Genomics Suppliers 

Plasmid DNA Purification 

• Thermo Scientific 

Fermentas 

• GE Healthcare 

Whole Genome 

Amplification 


Genomic DNA 

Purification 


• AnalytikJena 

RNA Purification 

• Fisher BioReagents 




• Vivantis Technologies 

• Invitrogen 

• TaKaRa 


• Miltenyi Biotec 

• TaKaRa 


Thermo Scientific Fermentas 

T 22 95 59 59 F 22 95 59 40 

PCR Amplification 


Finnzymes 


ABgene 


Fermentas 

• Fisherbrand 


Southern Blotting 


• Whatman 

• Carestream (Kodak) 


In-vitro Translation 

• Thermo Scientific Pierce 

Northern Blotting 


• Whatman 

Fermentas, part of Thermo Fisher Scientific, offers a 

broad range of product for all steps of your experimental 

workflow. 

• FastDigest® and conventional 

restriction enzymes 

• DNA/RNA Modification enzymes 

• Nucleic acid purification 

• PCR, qPCR and RT-PCR 

• Molecular weight ladders 

• Nucleotides 

• Molecular cloning 

• Molecular labeling 

• Transfection reagents 

• Fisher Bioreagents 

• Techne 

• Biometra 


• TaKaRa 

• RBC Bioscience 



• Carestream (Kodak) 


For over 30 years, Fermentas has been a world leader in the discovery, 

manufacturing and marketing of high quality molecular biology products. 


Fast Digest® 

Electrophoresis 


ABgene 

• Thermo Scientific Owl 

• Fisher Scientific 

• Hoefer 

• Grant 

RT-PCR, Q-PCR 

Amplification 

• SERVA 

• Biometra 

• Carestream 

(Kodak) 


• Thermo Scientific Finnzymes 

• Thermo Scientific ABgene 

• Thermo Scientific Fermentas 




• TaKaRa 


• ABI 

PCR Cleanup 

Purification of PCR Products and Restriction Fragments 


Fermentas 




The Fast Digest® enzymes are an advanced line of restriction enzymes for 

rapid DNA digestion. One big advantage of these enzymes are that they 

are 100% active in the universal Fast Digest® and the Fast Digest® Green 

buffers and that they are able to digest DNA in 5-15 minutes. This enable any 

combination of restriction enzymes to work simultaneously in one reaction 

tube, eliminating the need of perfoming sequnential digestions. 

Another improvment for your experiments are that the Fast Digest® Green 

buffer allows for direct loading of the reaction mixture on a gel, no need 

for extra loading buffer. The Fast Digest® Green buffer contains a density 

reagent and two tracking dyes. The blue dye migrates with 3-5 kb DNA 

fragments in 1% agarose gel and has an excitation peak at 424 nm while the 

yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel and 

has a excitation peak at 615 nm. 

Fast clone analysis 

Fast preparation of DNA for cloning 

Digestion of PCR products 

Fast RFLP genotyping 

Digestion of difficult-to-cleave DNA 

Microarray 

• Thermo Scientific NUNC 

• Corning 




Genotyping & 

Fragment Analysis 

• ABI 

Cloning 

Enzymes & Kits, Vectors 


Fermentas 


• TaKaRa 

• Fisherbrand 

• Eppendorf 

• IBA 


• ABI 

Electroporation 


Product Conventional Restriction Enzymes FastDigest® Restriction Enzymes 

Amount 

required to 

cleave 1 μg 

of standard 

Lambda DNA 

1 unit for 60 min 1 μl (rxn) for 5 or 15 min 

Buffer The Five Buffer System 

This system consists of 10X B (blue), 

G (green), O (orange), R (red) and 

Tango (yellow) buffers 


All (176) FastDigest® enzymes 

are 100% active in the universal 

FastDigest® and FastDigest® Green 

buffers 


28 

Genomics 

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Thermo Scientific PikoReal Real-Time PCR System - Fisher Scientific

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