Blood haematological and biochemical changes befor and after ...

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Sc. Parasit., 2009, 1-2, 59-62To make the leucocytes formula it is used Dia-Quik-Panoptic color. For the evaluation ofhaematological indices determination oferythrocytes was done through Turbidity method,hemoglobin determination by colorimetricmethod, and determination of total leukocytes wasperformed using Turk solution. The methods ofliver exploration. The methods of liverexploration included the determination of γ-glutamyltransferase activity using one reagent,and determination of alkaline phosphatase activityusing amethod with a single reagent. The resultswere interpreted in statistical terms using theMicrosoft Office Exel 2007.Results and discussionTo reflect the paraclinics changes occurring inbovine fasciolosis were performed serological andblood tests before and post-therapeutic after usingTolzan- Oxiclozanid 3,4%. The results obtained inthe two experimental groups are presented inTable 1:Table 1 – Blood and serological laboratory valuesBlood ComponentsLeucocitary formula (%)Liver enzymes (U/l)The values recordedbefore treatmentThe values recordedpost-treatmentTotal leucocyte(thousands/mm 3 )5335 5125Erythrocytes(million/mm 3 )7,556 4,669Hemoglobin(g/dl)13,248 9,732Lymphocytes 52,5 51,7Monocytes 4,5 1,9Eosinophils 16,7 13,5Neutrophil 24,8 29,3Basophils 0,1 0,2GGT 28,3 14,5PAL 96 76Following laboratory tests in group T beforetherapy total leukocyte values were5335(thousands/mm 3 ) and post-therapy are5125(thousands/mm 3 ). Values fall within thenormal bovine species(6), and before treatmentand after treatment differences obtained in groupT are not statistically significant(p>0,05).Erythrocyte values also were within normal limits(4,5). Pre therapeutic in T group values were7.556 million/mm³, and after treatment were 4.669million/mm³. Erythrocytes value are normal forbovine species(5,6). Differences between group Tbefore and after treatment are distinct statisticallysignificant (p
Sc. Parasit., 2009, 1-2, 59-62eosinophils in T group were 13.6 ± 8.708% andafter treatment were 13.5±6.974%. Increasednumber of eosinophils is actually an intolerancereaction of animal body, to the substancesdistributed through the parasites. It is noted thatlike the production of antibodies,hypereosinophilic reaction appears depending byterms of parasite contact with host tissues andtheir amplitudes responded. In generaleosinophilia reach the highest level during theinvasion of the body then diminishing during thelatency period. Ghergariu and colab.,2000,describe that eosinophilia is recorded forparasitism associated with hypersensitivityinduced by parasitic infestation. Antiparasiticmechanisms of eosinophilia place under theinfluence of signals from the extracellularenvironment. Eosinophils are mobilized andundergo transformation that increase performancefrom all points of view to be able to causeremoval of various substances that have inducedactivation. Hendrix C.M., 2003 describe that thereare 3 main ways in which eosinophilia acts on theparasite.• the mechanism of cytotoxicity cell mediatedAntibody-dependent, in this case eosinophilsmay be linked the target covered by variousimmunoglobulins;• eosinophils may be linked with the targetscovered by the C3b fragment of complement,it exacerbates binding functions, causingdamages the parasite membrane;• the interaction between IgE and eosinophils toobtain specific mediators, resulting inparasitic defense in two ways: localaccumulation of neutrophils and otherleukocytes, leukocyte activation and increasedtheir activity to damage parasites, and lastwould be action in the local tissue elements,intermediate local inflammation andproducing unfavorable conditions fordevelopment of parasites.Neutrophils and basophils values were withinnormal limits both pre and post therapeutic(4,5).Neutrophils number pre-therapeutic in the groupT were 24.8±9.097% and post-therapeutic29.3±11.602%(4,5). Differences between grouppre-treatment and post-treatment are statisticallyinsignificant (p>0.05). Neutrocitopenia is found inhepatosis, gastroenteritis, weaknesses.The values reported for the basophils pretherapeuticin the group T had value 0.1±0.3%and post-therapeutic 0.2±0.4%. Differencesbetween group pre-treatment and post-treatmentare statistically insignificant (p>0.05)GGT liver enzyme values in group T fall withinnormal limits for bovine species (8). Mean GGTvalue, before treatment are 23.8 U / L and aftertreatment 14, 5 U / L. Differences between antetreatmentand post-treatment group arestatistically significantly different(p
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