CHM 457 Biochemistry Laboratory Syllabus Winter 2009

Date Topic Chapter01/29 Th Purification of Alkaline Phosphatase 7Experiment 7-1 (day 3)-DEAE cellulose chromatography-enzyme assay on all fractions02/03 T Characterization of Alkaline Phosphatase 5Reading assignment: Chapter 5Experiment 5-2 (some reference to Experiment 5-1)-native gel and activity assay-protein determination-enzyme assay (if necessary)02/05 Th Enzyme Kinetics - Alkaline Phosphatase 8Reading assignment: Chapter 8Experiments 8-1 and 8-202/10 T Catch-up Day02/12 Th EXAM 1 (practical)Practical Based on Experiments 9-1 and 9-202/17 T Plasmid Isolation 11Reading assignment: Chapter 11 (pages 277-299) and Handouts-microscale isolation of plasmid DNA-DNA restriction incubation02/19 Th Plasmid DNA Restriction and AnalysisReading assignment: Handout03/03 T Catch-up Day03/05 Th Computer-Based PCR Primer Lab 12Reading assignment: Chapter 12 and Handout03/10 T PCR Technology 12Reading assignment: Experient 12-1 and Handout03/12 Th Characterization of PCR Product 12Reading assignment: Handout-introduction to subcellular fractionation03/17 T Subcellular FractionationReading assignment: Handout-preparation of homogenate-differential centrifugation03/19 Th Subcellular Fractionation 11-biuret assay-marker enzyme assay