Genetic Diversity of Aquilaria crassna - APAFRI-Asia Pacific ...

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Polymerase Chain Reaction• DNA (25 ng/l) 2 l• Primer F (10 M)0.5 l• Primer R (10 M)0.5 l• 10X PCR Buffer (15 mM MgCl 2 ) 1 l• dNTP (1 mM) 1 l• Taq DNA polymerase 0.1 l• Ultra pure water4.9 l• Total10 l
Polymerase Chain Reaction1.Pre-heat 94 ๐ C 3 min2. Denature 94 ๐ C 30 sAnnealing 50-64 ๐ C 30 sExtension 72 ๐ C 1 s28 cycles3. Final extension 72 ๐ C 5 min
Page 1 and 2: Genetic Diversity of Aquilaria cras
Page 3 and 4: ThailandAquilaria crassna Pierre ex
Page 5 and 6: Increasing demand andhigh price•
Page 7 and 8: Molecular markers• Biochemical ma
Page 9 and 10: ObjectivesThis study were to measur
Page 11 and 12: Collection of the sample41 Khon Kae
Page 13: Genomic DNA of agarwoodM 1 2 3 4 5
Page 17 and 18: Polymerase chain reaction(PCR)Elect
Page 19 and 20: DATA ANALYSIS• average number of
Page 21 and 22: Number of individuals in population
Page 23 and 24: Dendrogram
Page 25 and 26: Conclusion and suggestion• averag
Page 27 and 28: Provides data on the genetic divers
Page 29: THANK YOU FOR ATTENTION

Genetic Diversity of Aquilaria crassna - APAFRI-Asia Pacific ...

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