CGH as an Alternative to PCR-based Detection of Deletions in C ...

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MA plot (Hawaiian CB4856 / Bristol N2) – raw dataBefore Lowess NormalizationNon-Robust Lowess NormalizationM (log 2 ratio)M (log 2 ratio)A (intensity)A (intensity)
Our ProjectFind a better way to normalize this data• robust lowess normalization (iterative approach)• subset of probes for normalization to decrease the proportion of outliers(probes to the X chromosome only; probes to genes for which mutants arelethal)• “Supersmoother” variable-span smoothing (not very super)• rank invariant method• sequence information
Page 1 and 2: Normalization of High-Density Array
Page 3: Many Deletions in Strain from Hawai
Page 7 and 8: Robust Lowess vs SupersmootherLowes
Page 9 and 10: Genes with Larval Lethal RNAi Pheno
Page 11 and 12: Normalizing the whole genome with a
Page 13 and 14: EBarrays (LNN model) - Posterior Pr
Page 15 and 16: EBarrays (LNN model)- identified re
Page 17 and 18: EBarrays (LNN model)- identified re

CGH as an Alternative to PCR-based Detection of Deletions in C ...

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