SYKAM Chromatography Products - INSTRULAB

C H R O M AT O G R A P H YSYKAM Chromatography Products

HIGH PERFORMANCE LIQUID CHROMATOGRAPHYHigh Performance Liquid Chromatography (HPLC) is a form of liquid chromatography to separate compounds that are dissolvedin solution. HPLC instruments consist of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector.Compounds are separated by injecting a plug of the sample mixture onto the column. The different components in the mixturepass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase andthe stationary phase. InstrumentationSolvents must be degassed to eliminate formation of bubbles. The pumps provide a steady high pressure with no pulsating,and can be programmed to vary the composition of the solvent during the course of the separation. The liquid sample is introducedinto a sample loop of an injector with a syringe. When the loop is filled, the injector can be inject the sample into thestream by placing the sample loop in line with the mobile phase tubing. The different types of HPLC columns are described ina separate document. The presence of analytes in the column effluent is recorded by detecting a change in refractive index,UV-VIS absorption at a set wavelength, fluorescence after excitation with a suitable wavelength, or electrochemical response.Mass spectrometers can also be interfaced with liquid chromatography to provide structural information and help identify theseparated analytes.CHROMATOGRAPHSSykam Chromatographs are modular systems optimized to the customer‘s needs. The system compositionis set individually according to analytical needs. Every single system can be configured from simple routineHPLC instruments to highly sophisticated systems controlled by computer software for a multitude of possibleanalytical appliations. Any existing Sykam HPLC can be upgraded simply by adding additonal components.SOLVENT DELIVERYThe range of Sykam HPLC pumps include a varietyof different instruments from simple high pressureisocratic pumps to fully-programmable quaternarygradient pumps, also including low-pressure syringeand peristaltic pumps for specialized applications.SAMPLE INJECTIONThe sample injection of a Chromatograph can bedone either automatically by an Autosampler ormanually with a Manual Injection Valve.Autosamplers have the big advantage of automizedsample sequences, but also a higher reproducibilitythan manually injected samples.DETECTORSSykam offers the full range of HPLC detectorsfrom routine UV/Vis Detectors and RI Detectors tosophisticvated Electrochemical, Conductivity andFluorescence Detectors. With this range Sykam canoffer a solution for any application.The integrated gas supply can be used for degassingor for keeping solvents oxygen-free.Additionally, Sykam offers a Vacuum Degasser forkeeping your solvents gas free to insure a properworking of the Chromatograph.SWITCHING VALVESSykam offers a variety of motor switching valves tobe integrated into the Chromatograph.SOFTWAREFor data acquisition, integration and instrumentcontrol, Sykam offers software packages at differentprice and feature levels to meet the idnividualcustomer‘s needs.DEGASSER & REAGENT ORGANIZERThe Sykam Reagent Organizers, with integrated gassupply and valve-equipped bottles provide a cleansolution for storing solvents on your Chromatograph.

SYKAM S 1122 HPLC PUMPThe HPLC pump S 1122 is a dual plunger solventdelivery system. The mechanical design includesshort plunger stroke technology with only two checkvalves. This results in low pulsation, typically observedwith dual plunger pumps and high reliabilityotherwise achieved only by single plunger pumps.The delivery plunger performs a two mm stroke, thecompensation plunger makes one mm. The nearlypulseless solvent delivery of the pump results fromthe high stroke frequency and the use of the compensationplunger. The two plungers are counterrotating installed. While the delivery plunger pushesout the solvent volume, the compensation plunger,located on the pressure side, collects half of thevolume. During the return movement of the deliverypiston, the collected volume is pumped from thecompensation plunger.The microprocessor controlled speed regulationleads to a very stable speed of the D.C. motor,driving the plungers. Additionally, an integrated calculationsoftware continuously corrects the pressuredependent differential between the preset and actualflow rates. This results in a very constant volumedelivery throughout the entire range, independentlyof the resulting back pressure.Through the alphanumerical display, the pump‘sparameters are shown in clear text and the user canaqueous solutions, run time from 1 - 999 minutes,delay time from 1 - 999 minutes, time ramp for softstart,time ramp for soft-stop.Constant Pressure ModeConstant pressure, maximum flow in ml/min,runtime from 1 - 999 minutes, delay time from 1 - 999minutes, time ramp for soft-start, time ramp for softstop.High-Pressure GradientTwo S 1122 could be combined with a S 8200 HighPressure Mixer to form a High Pressure Gradientsystem.TECHNICAL SPECIFICATIONS Delivery Method: Dual-Piston Pump Operation Mode: Constant Flow /Constant Pressure Flow RateMicro:0.01 to 2.00 ml/minAnalytical: 0.05 to 10 ml/minPreparative: 0.2 to 40 ml/min Max. Pressure: 40 MPa (400 bar,6000 PSI) Flow Precision: < 0.1 % Flow Accuracy: < 1.0 % External Control: analoge signalcontrol & digitalcontrol (RS-232) Display:2x20 character LCD Dimensions: 297 x 198 x 495 mm Power:110/220 V, 50/60 Hzeasily control or change the data.The programming is easily done by a stepwisedialogue. There is basically a choice between twooperation modes. The delivery with „constant flow“,and the delivery with „constant pressure“. Normally,the constant flow delivery is used for analytical purposesand the constant pressure mode for specificoperations, e.g. column packing, etc. The followingparameters can be programmed for the respectivemodes:Constant Flow ModeConstant flow in ml/min, minimum pressure level(time delayed), maxiumum pressure level, compressibilityfactor from 0.7 (for methanol) up to 1.0 forORDER INFORMATIONCat.-No.Description10 10 046 S1122, analytical, Steel10 10 047 S1122, analytical, PEEK10 10 048 S1122, micro, Steel10 10 049 S1122, micro, PEEK10 10 050 S1122, praparative, Steel10 10 051 S1122, preparative, PEEK10 80 004 S 8200 High Pressure Mixer

SYKAM S 2100 SOLVENT DELIVERY SYSTEMThe HPLC Pump S 2100 is a compact eluent dosingsystem, upgradable from a high performance isocraticpump to a quaternary gradient pump with outstandingfeatures: menu programmable through large graphicaldisplay basic system: isocratic pump with three interchangablepump heads materials for the pump head: standard stainlesssteel, optional PEEK, Titan or PVDF Quaternary gradient operation gradient programmable in 0.1% steps for eachgradient A, B, C and D graphical display of the gradient curves integrated dynamic mixer with variable mixerspeed mixing sequence programmable 8 free programmable relay switching functionsfor auxiliary instruments Upgradable with integrated 4-channel degasser Diagnostic features storage of the pressureprofile for a gradient run and display of unregularitiesduring unattended automatic operations,e.g. pressure increase or pressure drop duringone runTECHNICAL SPECIFICATIONS Delivery Method: Dual-Piston Pump Operation Mode: Constant Flow /Constant Pressure Flow RateMicro:0.01 to 2.00 ml/minAnalytical: 0.05 to 10 ml/minPreparative: 0.2 to 40 ml/min Max. Pressure: 40 MPa (400 bar,6000 PSI) Flow Precision: < 0.1 % Flow Accuracy: < 1.0 % External Control: analoge signalcontrol & digitalcontrol (RS-232) Display:240x120-dot LCD Dimensions: 310 x 210 x 450 mm Power:110/220 V, 50/60 Hz Weight:~16 kgORDER INFORMATIONOptional: IntegratedVacuum DegasserCat.-No.Description10 10 067 S2100 Solvent Delivery System, analytical, Steel10 10 070 S2100 Solvent Delivery System, analytical, PEEK10 10 071 S2100 Solvent Delivery System, micro, Steel10 10 072 S2100 Solvent Delivery System, micro, PEEK10 10 073 S2100 Solvent Delivery System, preparative, Steel10 10 074 S2100 Solvent Delivery System, preparative, PEEK10 10 069 Option: 4-Channel Vacuum Degasser

SYKAM S 1521 PREPARATIVE PUMPThe pump operates with serial plunger techniquewith only one suction valve and one pressure valve.The pump heads are made of stainless steel (optinallyin PEEK) and come with integrated purgevalve. Control elements like flow rate, minimum andmaximum pressure levels can be easily controlledor changed, using the digital switches on the frontpanel. Furthermore, the LED display shows continuoslythe actual back pressure.One plunger stroke displaces 780 µl volume. Due tocomputer calculated cam plates with ntegrated precompressionprofile the remaining pressure fluctuationis reduced to a minimum. An excellent pressurestability is achieved even with flow rates of 3 ml/min.The electronic speed controller corrects automaticallythe pressure dependent solvent compressibility.Error messages and the pressure levels are availablethrough backside connectors for further controlpurposes.Independent of low or high flow rates and also independentof low or high pressure, the pump will deliverconstantly and free of mechanical or electronicaldisturbances (e.g. increase in temperature concerningthe motor part or control device). An optianlsolenoid valve makes a suction-side, variable sampleload of large volumes possible.For noise reduction, the two-stage driving mechanism(mounted on a solid bottom plate) is completetlyisolated from the pump case by an isolationfoam plate. Bearings and cam plates are permanentlylubricated from an oil reservoir. All componentswithin the pump coming in contact with liquidare placed over a collecting tray equipped with adrain pipeORDER INFORMATIONCat.-No.Description10 10 064 S1521 Preparative PumpTECHNICAL SPECIFICATIONS Delivery Method: Dual-Piston Pump Dimensions: Flow Rate: 3 to 300 ml/min Power: Max. Pressure: 200 bar Weight: External Control: analoge control500 x 180 x 280 mm110/220 V, 50/60 Hz~18 kg

SYKAM S 1610 SYRINGE DOSING SYSTEMThe S 1610 Syringe Dosing System uses 2 exchangeableglass syringes which are driven by steppermotors. This design allows a continuous and almostpulsation-free delivery of liquids with high precision.Syringe AStep 1 Step 2Syringe BSyringe A Working Principle(see the diagram below)While Syringe A delivers its volume to the system,Syringe B is filled from the reservoir through theswitching valve with high speed. As soon as SyringeA is emptied, the valve switches to Syringe B, whichimmediately starts delivering its volume. Syringe Anow starts with its filling cycle and the procedure isrepeated again. To make sure that the deliveringsyringe is ready when the valve is switched, thefilling syringe is drivenwith higher speed. Inthis way one syringe isalways filled completelywhen the other endsits delivery cycle. Thedelivering syringe startsa little bit earlier - beforethe valve switches - topre-compress the liquidfor constant delivery.Syringe BSwitchingValveSwitchingValvePumpOUTPumpOUTORDER INFORMATIONCat.-No.Description10 11 001 S1610 Syringe PumpTECHNICAL SPECIFICATIONS Delivery Method: Dual glass syringes External Control: Syringe Volume: 100 µl to 10 ml Display: Flow Rate: 800 µl/min up to 80 ml/min(depending on syringe used) Dimensions: Power: Max. Pressure: 25 bar Weight:analoge control4-character380 x 220 x 160 mm110/220 V, 50/60 Hz~8 kg

SYKAM S 7131 REAGENT ORGANIZERThe S 7131 Reagent Organizer is the optimal solutionfor storing your solvents. The integrated gas supplywith pressure regulator let you store the eluentunder inert-gas pressure, or for degassing purposesusing Helium.The organizer can hold up to four eluent bottles,which can be closed indivdually with the integratedvalve bottle caps.The S 7131 Reagent Organizer could be used withthe S 2100 Solvent Delivery System.ORDER INFORMATIONCat.-No. Description10 70 009 S7131 Reagent OrganizerTECHNICAL SPECIFICATIONS Gas Pressure: 0 to 7 bar Dimensions: Eluent Supply: 1 to 4 solvent bottles Weight:480 x 310 x 350 mm~7 kg SYKAM S 7121 REAGENT ORGANIZERThe S 7121 Reagent Organizer is the optimal solutionfor storing your solvents. The integrated gas supplywith pressure regulator let you store the eluentunder inert-gas pressure, or for degassing purposesusing Helium.The organizer can hold up to three eluent bottles,which can be closed indivdually with the integratedvalve bottle caps.The S 7121 Reagent Organizer could be used withthe S 1025/1122 Pump.ORDER INFORMATIONCat.-No.Description10 70 007 S7121 Reagent OrganizerTECHNICAL SPECIFICATIONS Gas Pressure: 0 to 7 bar Dimensions: Eluent Supply: 1 to 3 solvent bottles Weight:380 x 330 x 350 mm~3 kg

SYKAM S 7505/7510 VACUUM DEGASSERThe degassing of solvents is very important inliquid chromatography, as oxygen and other inthe solvent dissolved gases affect the analysis forvarious reasons; through the volume contraction(gradient mixing), different pressure ratio (in frontand behind the column) and caused by differenttemperatures, the dissolved gases are released incourse of time. This will influence the reliability ofthe analysis (pump) as well as the detection efficiency(measuring cell). Therefore, dissolved gaseshave to be removed from the solvent.Operation Principle of the DegasserIn a vacuum chamber, the solvent to be degassedis distributed into several, parallel arranged, semipermeablemembrane chambers. When a highvacuum (more than -850 mbar) is applied, only thein the liquid dissolved gases are diffusing throughthe thin membrane. With a total solvent volume of8 ml per degassing channel, the Sykam DegasserS 7510 achieves an extremely low remaining gasconcentration of 0.5 to 5 ppm oxygen, dependingon the degasser‘s flow rate (0 to 10 ml/min). With agiven flow rate, the degassing quality can be furtherincreased by coupling two or several membranechannels.The digital display of the actual vacuum in Milibarmakes the Sykam Degasser S 7510 the first vacuumdegasser on the market which functions can bechecked and documented at any time.On top of that, the actual vacuum run can be continuouslyregistered via an analogue DC output (-1V for 1000 mbar). Through in integrated logic, anyleakage in the vacuum system is detected (run timeof the vacuum pump for defined vacuum range) andis not only optically displayed by a controll LED butis also indicated through a relay contact closure forperipheral systems. For removing possibly existingsolvent traces, the vacuum pump‘s waste gasescan be directed via a fixable tube connection tothe laboratory‘s ventilation. The Teflon membranepackages are exchangable, in case of damage.ORDER INFORMATIONCat.-No.Description10 71 001 S 7510 Degasser, 2-Channel10 71 002 S 7510 Degasser, 3-Channel10 71 003 S 7510 Degasser, 4-Channel10 71 004 S 7505 Degasser, 2-Channel10 71 005 S 7505 Degasser, 3-Channel10 71 006 S 7505 Degasser, 4-ChannelTECHNICAL SPECIFICATIONS Degassing Method: Applied Vacuum Display: Efficiency: 0.5ppm oxygen Dimensions: Flow Rate: max. 10 ml/min Power: Volume/Channel: 8 ml Weight:4-character110 x 150 x 350 mm110/220 V, 50/60 Hz5.6 kg

SYKAM S 3580 RI DETECTORThe S 3580 Differential Refractive Index Detectorseries offers the sensitivity, stability and reproducebilityrequired for optimal RI detection.The thermal isolated optic with a countercurrentheat exchanger and with its programmable temperaturecontrol, results in an extremly stable baselineand an optimal Signal / Noise ratio.The S 3580 series provide autopurge and autozerocapabilities, as well as RS232 communication toacquire data directly without using any external signalinterface. S 3580 detectors are available for:- micro- analytical- semi-preparative modeTECHNICAL SPECIFICATIONSMicro Analytical Semi-Preparative Detection Method:Deflection Refractive Index Range: 1.00 to 1.75 Flow Rate: 0.2 to 3.0 ml/min 0.2 to 3.0 ml/min 1.0 to 50 ml/min Flowcell Volume: 4 µl, 45° 9 µl, 45° 7 µl, 5° Flowcell Pressure: 6 kg / cm² 6 kg / cm² 6 kg / cm² Dead Volume: 6 µl 24 µl 88 or 353 µl Linearity Range: 0 - 500 µRIU 0 - 1,000 µRIU 0 - 20,000 µRIU Noise Level: 10 x 10 -9 RIU 5 x 10 -9 RIU 10 x 10 -8 RIU Drift (1.0 ml/min H 2O): < 1 mV / hour < 1 mV / hour < 1 mV / hour Integrator Output:+/- 1 Volt Digital Interface:RS-232; Purge; Autozero; Start; Stop; Data Out 1Hz, 10 Hz; Lock Digital Output:TTL: Intensity Alarm Digital Input:TTL: Purge, Autozero, Start/Marker Temperature Setting: Ambient, 35 °C to 55°C in 1°C steps; Thermal Fuse @ 75°C Time Constant:RAW (0.0 sec), Fast (0.4 sec), Medium (0.8 sec), Slow (1.2 sec) Dimensions:220 x 350 x 155 mm Power Supply:110/220 V; 50/60 HzORDER INFORMATIONCat.-No.Description10 31 003 S3580 RI Detector

SYKAM S 3210 UV/VIS DETECTORDiode Array Technology, Photoconductor Opticsand Dual Lamp Source. Diode Array Technology for wavelength changewithout any mechanical moving parts. Online scan of wavelength and collection ofspectra data without stopping the flow or interruptingthe running analysis (optional). Dual lamp (deuterium/tungsten) for a spectralrange of 190 - 720 nm. Integrated peak detector with programmabledelay time. Integrated soolvent recycling system (3-waysolenoid valve optionally). External START, external AUTOZERO via TTLsignal. Serial interface (RS232C) for external control. Programmable change of wavelength (up to 10steps). Full DAD operation mode with ChromStar software(optional).TECHNICAL SPECIFICATIONS Optic System: 256-Diode Array Features: Wavelength: 190 to 720 nm Bandwidth: 2 nm per Diode Range:0.0005 to 2.0 AU Display: Drift:

SYKAM S 3240 UV/VIS DETECTORDiode Array Technology, Photoconductor Opticsand Dual Lamp Source. Diode Array Technology for wavelength changewithout any mechanical moving parts. 4-Channel measurement with separated analogoutput (0 - 1000 mV) Online scan of wavelength and collection ofspectra data without stopping the flow or interruptingthe running analysis. Dual lamp (deuterium/tungsten) for a spectralrange of 190 - 720 nm. Integrated peak detector with programmabledelay time. Integrated soolvent recycling system (3-waysolenoid valve optionally). External START, external AUTOZERO via TTLsignal. Serial interface (RS232C) for external control. Programmable change of wavelength (up to 10steps). Full DAD operation mode with ChromStar software(optional).TECHNICAL SPECIFICATIONS Optic System: 256-Diode Array Features: Wavelength: 190 to 720 nm Bandwidth: 2 nm per Diode Range:0.0005 to 2.0 AU Display: Drift:

SYKAM RF10 FLUORESCENCE DETECTORThe RF-10AXL is based on a further developmentof the well known RF-551 and RF-10AxL. Improvedsignal to noise ratio (>300) for the RAMAN Peakand extended wavelength reproducibility are themain features of this instrument. For better performancethe signal processing in the instrument hasbeen improved.New designed flow cell stand for better pressureresistance and minimize noise due to air bubbles.For safety of the system a leak sensor protect forsolvent leaks.The RF-10AXL supports several validation features(e.g. lamp energy test, counter functions and internalwavelength check) for work under strong GLP /GMP guidelines.TECHNICAL SPECIFICATIONS Light Source: High Precision Xenon Lamp Monochromators: Concave, blazed holographicgrating monochromators Wavelength: 200-650 nm(200-900 nm optional) Bandwidth: 15 nm Accuracy: ±2 nm Reproducibility: ±2 nm Sensitivity: S/N ratio 300 for Raman lineof distilled water(350 nm excitation; 1.5 sec.) Detector: Photomultiplier Cell Volume: 12 µl Cell Pressure: ~2 MPa Cell Material: SUS316, Quartz, PTFE Programming: up to 32 Wavelength steps Dimension: 260 x 520 x 205 mm Weight:17 kg Power:100-240V, 350 VA, 50/60 HzORDER INFORMATIONCat.-No.Description10 90 087 RF10 Fluorescence Detector

SYKAM S 5200 AUTOSAMPLERThe system can be upgraded at any time from the basic version forfixed volume injection with sample loop overfilling, up to variable volumeinjection mode for variable volumes from 1 - 100 µl (optional up to500 µl) without changing the sample loop.With the next upgrading step, the S 5200 can be used for fully automaticsample processing fro pre-column derivatization. In the derivatisatingmode, up to three independent reagents can be added to aspecified sample volume, mixed with sample and programmed withindependent reaction times. In addition, the sample can be diluted withmore than 100 times (depending on sample volume and syringe volume).For temperature sensitive samples, the sample tray can be cooled orheated with the optional cooling system.For chemical inert operation, the instrument can bedelivered for fully metal-free sample handling (completelyequipped with PEEK and FEP materials,including sample needle).A wide range of sample racks and sample vials canbe used for operation from 1 µl up to 5000 µl or byusing micro titer plates.Simple operation through large alphanumeric displaymenu.ORDER INFORMATIONCat.-No.Description10 50 001 S5200 Autosampler, Fix Volume10 51 001 Update: Variable Volume10 51 002 Update:Derivatisation10 51 003 Option: Heating/Cooling10 51 004 Option:Inert Version (PEEK)10 51 005 Option:Preparative VersionTECHNICAL SPECIFICATIONS Sampling System: X-Y-Z-Operation; steppermotor driven syringe Sample Capacity: 120 vials in 2 racks á 60 Sample Loop: standard: 20µl Material: Stainless Steel or PEEK Reproducibility: Fix Volume: < 0.5%Variable Volume:

SYKAM S 4011 COLUMN THERMO CONTROLLERNot only the optimization of separations but alsoa constant temperature of the separation columnplays a very important role in the reproducibility ofthe retention times. The Column Thermo ControllerS 4011 was especially developed in order to overcomethese kind of problems. With the help of thebuilt-in Peltier elements, it is ossible to keep thetemperature of the columns between +5° C and+70° C independent of the ambient temperature.A precise column cooling system for programmablefluorescence detection and for the optimization oflater eluting components is of great advantage.Especially separations which are very temperaturedependent, like gradient elution of polycyclic aromatichydrocarbons, greatly profit by this instrument.The integrated Peltier elements provide exacttemperature control. Therefore, fast temperaturechanges are possible, which are needed for temperaturegradients.Furthermore, the Column Thermo Controller S 4011can not only be used for heating, but also for coolingbelow ambient temperature.Gas Sensor Safety FeatureAt the inside of the column chamber a gas sensoris installed for detecting flammable gases. Shouldthere be a leakage during operation, the yellow LEDwill indicate the leakage and the oven is automaticallyswitched off.ORDER INFORMATIONCat.-No.Description10 40 007 S4011 Column Thermo ControllerTECHNICAL SPECIFICATIONS Temp. Element: Integrated Peltier Elements Safety Features: Chamber Size: 35 x 35 x 300 mm Temp. Range: +5° C to +70° C Dimensions: Temp. Accuracy: ±0.5° C Temp. Constancy: ±0.1° C Power: Temp. Selection: 1° C steps Weight: External Control: analoge control Display:2-digit 7-segmentGas Sensor f. flammablegases, overheat protectionController: 220x150x345mmOven: 135x180x510mm110/220 V, 50/60 HzController: 6.3 kgOven: 9.4 kgColumn Chamber with Gas Sensor

SYKAM S 433 AMINO ACID ANALYZERThe innovative automatic Amino Acid Analyzer S433 combines the advantages of the classical ionexchange separation method with the modern techniqueof high performance liquid chromatography. Thecomplete package of sophisticated instrumentation,a wide variety of prepacked and tested separationcolumns, combined with optimized ready-to-use buffersolutions and chemicals, creates the right answerfor any routine or research problem in amino aciddetermination. More than 30 years experience indeveloping and operating sophisticated amino acidanalyzers results in unmatched performance. Multistep SeparationOnly two or three buffer solutions have to be combinedto form the best optimized buffer profile at anypart of the separation program. No more compromisesby the limitation to four or five buffer changes. Cooled Reagent StorageAll buffer solutions, as well as the Ninhydrinereagent are All buffer solutions, as well as theNinhydrine reagent are stored under inert gas pressurein a refrigerated cabinet to avoid oxidation andair contamination independent of the ambient environment. Integrated Autosamplerwith a capacity of 120 vials in a cooled sample tray.Volumes from 1µl to 100 µl can be injected withoutany loss of sample. The injected volume isadjusted by a high precision syringe, driven by astepper motor with a resolution of 17 steps per µl.A programmable wash program will flush the entireinjection system to avoid cross contamination of thesample. Integrated Vacuum Degasseravoids the interruption of the buffer pump by airbubbles without the need of bubble traps withvarying volumes, causing changes in retention timesof the different amino acids. Separation Column Ovenintegrated solid state column oven with fast heatingand cooling capability with a temperature rangefrom +5°C to +85°C independent of the ambienttemperature. Up to twelve programmable temperaturesteps can be used during one separation program. High-Temperature Reactorwith a programmable temperature range from ambientto 185°C with a coiled capillary for the colorreaction of the amino acid-ninhydrine complex.Automatic flushing of the reactor coil with a washingsolution after each run prevents the blockage of thecapillary. Integrated Reagent Dosing Pumpfor Ninhydrine delivery and flushing of the reactioncoil after each run. Programmable flow rate from0.01 to 2.0 ml/min. Complete Inert DesignAll materials coming into contact with the buffersolutions All materials coming into contact with thebuffer solutions and reagents are made of inertmaterials as PEEK, PTFE, PVDF etc. Therefore,there is no need for using special non-corrosive buffercompositions or reagents. Integrated Dual-Channel Photometerfor the amino acid detection at 440 nm and 570 nm

wavelengths.Summing option for both channels, for single channelintegration of all amino acids. Consumablesready-to-use Buffer Solutions, newly formulated Ninhydrine Reagentwith long-term stability and Separation Columns. All consumables areready-to-use, tested, and certified to guarantee the highes quality standard. Optional Application- Post column derivatisation with OPA (needs an optionalFluorescence Detector)- Carbohydrate determination for reducing sugars with Cu-bicinchoninatepost-column derivatisation (except the separation column, noadditional extras are needed)- Polyamine determination with Ninhydrine or OPA postcolumn derivatisation.S430 Amino Acid AnalyzerAmino Acid Analyzer FlowchartORDER INFORMATIONReady-Made Buffer Solutions,Reagents & ColumnsCat.-No.Description11 20 001 S433 Amino Acid Analyzer11 20 002 S433 Amino Acid Analyzer w. Data System: PeakSimple11 20 002DS S433 Amino Acid Analyzer w. Data System: ChromStar11 20 003 S430 Manual Amino Acid Analyzer11 20 004 S430 Manual Amino Acid Analyzer w. Data System: PeakSimple11 20 004DS S430 Manual Amino Acid Analyzer w. Data System: ChromStar10 41 002 S4300 Amino Acid Reaction Module

SYKAM S 135 ION CHROMATOGRAPHY SYSTEMIon Chromatography is one of the mostimportant methods for the determinationof alkaline, alkaline earth and transitionmetals, inorganic anions, sulfuric compoundsof different oxidation levels, organicacids, and various tensides. Indeed,for aqueous solutions Ion Chromatographyis one of the most sensitive methods forthese substances, since in most cases thesamples can be injected into the systemwithout time consuming sample preparation.If required, e.g. for trace amounts,enrichment can be done within the chromatographicsystem.The Sykam S 135 is a compact system with modularsetup. Even the basic system is designed formost sensitive anion analysis employing suppressionof eluent conductivity.The column oven integrated into the system holdsmeasuring cell, pre-column, separation column, andsuppression columns, and thus guarantees accurateretention times and quantification with superiorreproducibility. the UV/Vis detector with variable wavelength forhigh sensitivity determination of nitrite, organicacids, or transition metals after PAR post-columnreaction. the electrochemical detector for detection of sulfide/cyanideor carbohydrates. automatic concentration units for trace analysis.The conductivity detector‘s outstanding features arehigh background suppression, baseline stability, andsignal linearity over a range of several decades.These characteristics become especially importantwhen single column techniques are employed, e.g.for the determination of alkaline ions and alkalineearths.The chemically inert pump with its pump headmade of PEEK meets all the requirements of IonChromatography. It has two serial pistons thus deliveringwith extremely low pulsation. This pump caneven be used for HPLC, and by just adding a gradientmodule the system in fact can be easily upgradedfor any HPLC application.Depending on applicational needs or analytical problems,the Sykam Ion Chromatograph can be easilymodified by integrating additional Sykam systemcomponents. E.g. there are: switching valves to select columns or eluents forrapid method change.ORDER INFORMATIONCat.-No.Description11 11 002 S135 Ion Chromatography System10 65 004 S4260 IC Suppression Module10 30 003 S3115 Conductivity Detector

SYKAM DATA SYSTEM: CHROMSTARChromStar is one of the first MS-Windows-based chromatography data system. It was already presentedunder Windows 2.0, by Bruker-Franzen. Today, ChromStar has become a software with a recent conception,developed in C++, and it is compatible with the user interface of Windows 2000. Thanks to the great flexibilityof ChromStar, all tasks of liquid chromatography can be carried out easily, in agreement with GMP/GLPrequirements. HardwareThe A/D converter boards are designed as PC pluginmodules. The ChromStar data system is completelybuilt up in only one PC, requiring no furtherexternal components. Up to four independent dataacquisition channels can be installed. Via a busorientedcontrol interface designed exclusively forChromStar, a multitude of chromatography instrumentsand individual modules of various manufacturerscan be controlled without need to care abouttheir special properties. Integrating converters with aresolution equal to 30 bits guarantee the acquisitionof chromatograms with the highest possible precision.Raw data are saved in regular intervals avoidingdata loss due to computer crash or power failure.No special PC system is needed. The requiredmemory is only dependent from the operating system,an average chromatogram needs approximately100 kBytes hard disk memory. The computerhandles ChromStar as a Windows application,therefore the user benefits from all computer-networkadvantages without limitations. SoftwareThe ChromStar software includes the following features: GLP guidelines are fully supported. One to threeuser levels and password protection enable restrictionof access.ChromStar: Integration Unlimited number of data points per chromatogram. Acquisition of negative input signal and considerationof negative peaks. Easy handling via function buttons. Chromatogram and report preview alreadyduring file selection. Labelling of chromatograms with peak numbers,retention times, peak names, and/or any texts. Automatic, manual, and combined integration,i.e. directly from the reintegration screen, automaticallyintegrated chromatograms can be integratedmanually as well. GLP-conforming presentation of the originalanalysis from stored data. Grouping and summation of individual peakgroups within a chromatogram simplify the analysis. Serial analysis of great chromatogram numbers. Three-dimensional multiple chromatogram presentation. Multipoint calibration plots of the first, second,and third order. Numerous tools simplify the creation of the peaktables required for quantative analysis: all oreselected data from results lists can be integratedusing import, copy, and paste functions; a graphicpeak-table editor enables the exact adjustmentof the peak window fitting to the actualchromatogram, via reading and presentation ofchromatogram series, the variance of the retentiontimes is taken into account. Ratio, sum, difference calculations, and the concatenationof two chromatograms. Smooth rescaling and inverting of chromatograms,calculation of the first and second derivation,digital filter functions for noise reduction. Spke removal under consideration of the actualpeak width, peak enhancement for calculatoryimprovement of peak separations and for theidentification of hidden peaks. Automatic or manual baseline correction of chromatogramswith baseline drift. Graph adjustment to non-resolved peak groupsby Gaussian functions or fractional rational

part 1 (calibration of the 1st to 9th order). Report EditorChromStar DAD: 3-D Plot (Coffee Sample)The report editior generates printer reports definedaccording to your special needs. By selectingobjects, like chromatogram, result list, parametersfor data acquisition and analysis, date, samplename, graphics, external files, and more, the usercan define individual templates.Based on these templates, ChromStar generatesthe printer report, and the current parameter valuesare automatically included.All standard printer outputs are available as standardtemplates, and they can be modified and adaptedas well. MultitaskingChromStar DAD: Density Plot (Coffee Sample)The option to run several programs simultaneouslyunder Windows enables the user to workwith ChromStar as an integration tool from otherprograms using DDE data transfer. For instance,simple programs for sample preparation or sampledosage can be performed, and, at a predefinedtime, ChromStar sets up a chromatogram withoutuser interference.This technique has been used to develop the fullyautomatic method optimization program Summitand programs for fully automated chromatographiccontrol.functions for the analysis of strongly overlappingpeaks. Data Transfer:With the MS Windows Clipboard, the Windowsmetafile export, and the standard data formatsgenerated with ChromStar, it is easy to transfergraphic and numeric results into other applications,like word processing, drawing, DTP, database and spreadsheet softwares, e.g. MS Excel,CorelDraw. Control of HPLC instruments like pumps, injectors,autosamplers, detectors, and columnheaters of various manufacturers via analogor serial control using control interfaces and/orSTART/STOP/READY communication. Switching output activation of up to 12 differentexternal instruments like pumps, fraction collectors,valves. Calculation and graphic presentation of the differentialand integral distribution curves (Mw, Mn,Mz, Mz+I) for the total integration range or partsof it. Calibration according to DIN standard 55672,ORDER INFORMATIONCat.-No.CSCS-LCS-DADCS-UPD4CS-MULTCS-MCSCS-MCADescriptionChromStarChromStar LightChromStar DADUpdate: 2-Channel A/D BoardMultiCom BoxMultiCom Interface, SerialMultiCom Interface, Analoge

SYKAM DATA SYSTEM: PEAKSIMPLEPeakSimple chromatography software combines quick learning, ease of use, and convenient powerful featuresfor GC and HPLC and CE. PeakSimple’s intuitive graphical functions and features are so user-friendly mostoperators can produce results almost immediately and without specific training. PeakSimple is packed withstate of the art features like drag-able retention time windows, seldom found in other data system softwarepackages costing much more. With PeakSimple, no hardware is installed in your computer, so you can use asmall portable notebook instead of a full-sized computer. If you travel with your system or have limited benchspace you will appreciate this. Multiple users can also connect their individual computers to the chromatographwithout moving the data system hardware from computer to computer. Baseline Subtraction & Data SmoothingBlank baseline subtraction is useful to compensatefor baseline drifting due to column bleed and temperatureramping. PeakSimple lets you subtract baselinesin real time as data is collected or post run.Noisy detector signals can be smoothed manuallyor automatically at the end of a run. Smoothingalgorithms include Olympian, Moving Average, andSavitsky-Golay. CalibrationMulti-Level Calibration CurvesCalibrate peaks six ways (multi-line, quadratic, parabolic,etc.) using single or averaged data at up toseven concentration levels. Statistics for evaluatingline fit quality, modification date audit trail, and curveprintout help to ensure defensible results.Calibration AveragingPeakSimple allows up to three replicate calibrationstandards at seven levels of concentration to beaveraged when constructing calibration curves. Channel DetailsEach channel has a Channel details dialog boxwhich allows users to set parameters for that particularchannel. From the Channel details dialog box,you can set your sampling rate and default millivoltdisplay limits; choose temperature, pressure, orgradient control; subtract the baseline from anotherchannel; overlay the data from another channel; turnData-logger mode ON or OFF; designate a starttime to compensate for relative retention shifts, andmore. ComponentsCreate, save and edit component tables with anunlimited number of compounds. Enter expectedretention times, control peak display, and more!Component details may be viewed and edited bydouble-clicking on any retention window in the chromatogram.Or, open the Component table and doubleclick on any component in the list to view or editthat component’s details. External Event ControlIn addition to performing timed integration events,control up to eight external contact closure relayoutputs to actuate sampling valves, autosamplers,solenoids, pumps, or any external device using TTLor relay contact closure triggers. Dynamic Data Exchange (DDE)Link PeakSimple to your DDE compatible spreadsheetor word processor (Excel, Word, 123, etc.).Anaytical results are automatically transferredafter every run, or can be accumulated withinPeakSimple and copied as a block of data. Use theCopy Picture option to paste the chromatogram itselfinto Excel, etc. along with the results. IntegrationUse the Integration button to determine howPeakSimple integrates the data peaks in the chromatogram.Set peak detection sensitivity, areareject and standard weight. Specify a spike channel,merge results from another channel, and correct forsample weight and dilution. Data Logger ModePeak Simple’s Data Logger Mode allows you to displaya scaled and calibrated result in large numbersinstead of the usual strip chart data presentation.Data Logger Mode is especially useful when monitoringtotal hydrocarbons on one channel while performinga separation on another channel. Manual IntegrationManual integration tools permit you to refine the

integration method applied to any peak. The manualintegration toolbox is available at a click of themouse. Baseline projection may be „rubberbanded“from point to point, forced to a valley, dropped vertically,skimmed, etc.The Manual Integration toolbox may be placedabove (as shown at right) or to the left of the chromatogramwindow. It may also be dragged „off“ thechromatogram window to float as an independent,move-able window. Merge Results from mutiple ChannelsPeakSimple lets you merge the results from anychannel or all channels into one report. This featureis handy when you’re combining results from differentdetectors into a single report for export to Excelor other data analysis programs.„Temperature,“ „Pressure,“ and „Gradient“ channelcontrol options all use the same simple dialog box,and each may be programmed with unlimited rampsand holds. Program one or two SRI GC columnovens from ambient to 400° C with 0.01 degreeresolution and negative programming. Program thecarrier gas pressure on SRI GCs equipped withelectronic pressure control. Form binary HPLC gradientsfor low-cost pump control. Validating HardwarePeakSimple will play back and reacquire any chromatogrammultiple times, establishing the precisionand accuracy of the data system using real data,not „canned“ chromatograms. PeakSimple’s validationcan be performed by the user anytime withoutextra hardware. Overlay ChromatogramsOverlay the data in any channel onto any otherchannel for retention timecomparison or multi-detectorcorrelation.The Overlay Adjust feature lets you stretch and shiftoverlaid data to facilitate pattern matching. PostrunPeakSimple can perform a variety of postrun actionsto help you maintain and organize your data.Specify how data will be saved, and automaticallyadd the results of the run to the results log for thatchannel. You can set PeakSimple to automaticallyprint the results at the end of a run, and update yourDDE link. Execute a command, specify a recalibrationlevel, and restart the run after a given amountof time. You can even have PeakSimple smooth thedata before copying it into another channel. PrintUse any Windows supported color printer to createimpressive hardcopies of your chromatographicdata. Print multiple chromatograms per page foreasy detector-to-detector comparisons and paperworkconsolidation. Print overlaid data in contrastingcolors with adjustable line weight. Queue ProcessingCreate customized autosampler sequences forliquid injections, purge and trap autosamplers, gassampling valves, and stream selectors including uniquepredefined sample information, auto-calibrationand batch reprocessing of previously run samples. Temperature/Gradient ProgrammingORDER INFORMATIONCat.-No.PS-1CPS-4CDescriptionPeakSimple, 1-ChannelPeakSimple, 4-Channel

SYKAM DISTRIBUTORSCHINABeijing JieShengYiKe Science & TechnologyDevelopment Ltd.No. 3 B Longtan StreetChongwen District, Beijing 100061ChinaPhone: 010-64845885 or 010-64845891FAX: 010-64845891Mobile: noneeMail:Homepage:EGYPTChromaTech15 El-Taiaran Street Ext.,Cairo, Egypt 11471market@jsykkj.comhttp://www.jsykkj.comPhone: 00202 312 33 68 or 262 09 24FAX: 00202 710 321 5 or 405 22 93Mobile: noneeMail:Homepage:HUNGARYchromatech@link.netnoneSpectrobal Merkantil Kft.Üllöi ut 1211091 BudapestHungaryPhone: 0036 1 215 42 45FAX: 0039 1 215 42 45Mobile: noneeMail:Homepage:ITALYTecnogalenica s.r.l.Via Vicenza 220063 CernuscoItalyinfo@spectrolab.huhttp://www.spectrolab.huPhone: 0039 02 92 10 67 81FAX: 0039 02 92 10 72 23Mobile: noneeMail:Homepage:nonenoneJORDANArab Medical & Scientific AllianceKhalil Salem Str. Tela El-AliP.O. Box 2509 Tela Al Ali 11953JordanPhone: 00962 6 55 28 009-421FAX: 00962 6 55 32 358Mobile: 00962 79 66 99 229eMail:Homepage:KUWAITams@ads.com.jononeAdvanced Technology Company K.S.C.Opp Al Shaab Leisure ParkMaidan HawalliP.O. Box 44558Hawalli 23060KuwaitPhone: 00965 88 55 99 - 277FAX: noneMobile: noneeMail:Homepage:LEBANONCaretek S.A.L.nonenoneJean Dominique Ayoub St.Ville Dieu Center - 5th FloorAchrafieh - Beirut - 2064 7505LebanonPhone: 00961 1 61 39 99FAX: noneMobile: 00961 3 90 10 57eMail:Homepage:nonehttp://www.caretek.com.lb

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Sykam GmbHSysteme & Komponenten analytischer MeßtechnikGewerbering 1586922 EresingGermanyTel.: ++49 (8193) 93 82 - 0FAX: ++49 (8193) 93 82 - 20EMail: Info@sykam.comWeb: http://www.sykam.com