Elicitation effect of Saccharomyces cerevisiae yeast extract on ...

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N. Happyana et al. Proceeding <strong>of</strong> The International Seminar on Chemistry 2008 (pp. 257-261)Jatinangor, 30-31 October 2008ControlmediaSamplemediaFigure 2 Chromatogram comparison <strong>of</strong> the sample media and the control mediaTable 1 Comparison <strong>of</strong> HPLC chromatogram peak area for analysis <strong>of</strong> the <strong>effect</strong> <strong>of</strong> elicitation <strong>of</strong> S. <strong>cerevisiae</strong><strong>yeast</strong> <strong>extract</strong> elicitor toward production <strong>of</strong> secondary metabolites <strong>of</strong> M. macroura Miq 509 hairy rootculturesCompoundsRetention timePeak Area (mAU)(minutes) Sample Control Sample media Control mediaA 10.987 3484 1475B 14.315 8793C 25.792 7507 5367 12968 9726D 26.411 7069 2310E 26.741 49053 3694 7361 5585F 26.976 29641 7410 26176 4772G (new compound) 27.672 24244 8082 33768 6753H 28.224 15594 1432 2512 628I 28.469 172364 7388 36839 12950J 28.789 9546 3694 100492 4762K (mulberr<strong>of</strong>uran P) 29.045 136973 19407 84005 18484L 29.237 2576 5768N (chalcomoracin) 30.197 360453 228515 364330 92929O 31.168 22213 33667 14822 8481P 32.075 32853 11082 22203 3173Q 33.897 9152 3425 3665 1537R 20.8 10381S 23.589 7393 3554T 24.233 3693 2129U 29.696 2765 6455 1581260
N. Happyana et al. Proceeding <strong>of</strong> The International Seminar on Chemistry 2008 (pp. 257-261)Jatinangor, 30-31 October 2008ConclusionsThe ellicitation <strong>of</strong> <strong>Saccharomyces</strong> <strong>cerevisiae</strong> <strong>yeast</strong><strong>extract</strong> elicitor (0.5 ml; 2.5% w/v) on hairy rootcultures <strong>of</strong> M. macroura Miq. 509 has increased most<strong>of</strong> secondary metabolite content. Based on HPLCanalysis, the elicitation has increased production <strong>of</strong>mulberropuran P (705 %), chalcomoracin (157%), anda new compound (299%). Beside that, accordinghuman ovarian carcinoma cell line (OVCAR-3) assay,generally the elicitation has increased the production<strong>of</strong> secondary metabolites which have cytotoxic activityagainst OVCAR-3 significantly.AcknowledgementsWe thank the Biotechnology Laboratory, IndonesianInstitute <strong>of</strong> Sciences, for the transformation <strong>of</strong> thehairy root cultures. We are grateful to Pr<strong>of</strong>. Dr. OliverKayser, University <strong>of</strong> Groningen, Netherlands for theHPLC measurements.ReferencesDesphande V. H. 1975.Indian J Chem. 13:453.Ferrari F., Monache F. D. 2001. Fitoterapia. 72:301Hakim E. H., Achmad S. A., Makmur L. 1997.Proceedings Joint Seminar ITB-UKM. 2:21.Hano Y., Suzuki S., Nomura T., Iitaka Y. 1988.Heterocycles. 27: 2315Hano Y., Nomura T. 1986. Heterocycles. 24:1381.Heyne K. 1987. The useful Indonesian plants.Research and Development Agency, Ministry <strong>of</strong>Forestry:Jakarta, Indonesia.Nomura T., Hano Y. 1994. Nat Prod Rep. 11:205.Nomura T. 1988. Prog Chem Org Nat Prod. 53:88..Syah Y. M., Achmad S. A., Ghisalberti E. L.,Hakim E. H., Iman M. Z. N., Makmur L.,Mujahidin D., Soekamto N. H. 2000. Fitoterapia.71: 630.Syah Y. M., Achmad S. A., Ghisalberti E. L., HakimE. H., Makmur L., Mujahidin D., Soekamto N. H.2004. Journal <strong>of</strong> Chemical Research. p. 339Takasugi M., Nagao S., Masamune T., Shirata A.,Takahashi K. 1988. Chem Lett. p.1573.Takasugi M., Nagao S., Masamune T., Shirata A,Takahashi K. 1978. Tetrahedron Lett. 9:797261
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