NucleoSpin® Gel and PCR Clean-up - MACHEREY-NAGEL ...

DNA, RNA, andprotein purificationCatalog supplement 2013www.mn-net.com

ContentsMACHEREY-NAGEL · Bioanalysis catalog supplement 2013This Bioanalysis catalog supplement 2013 is an addition to the main Bioanalysis catalog 2011 / 2012.Introducing 11 new products and presenting 4 product updates, the catalog supplement 2013 shows alllatest MACHEREY-NAGEL Bioanalysis developments at a glance.ContentsPlasmid DNAPage CategoryTransfection-grade plasmid DNANucleoBond ® Xtra BAC 2 NEWTransfection-grade, endotoxin-free plasmid DNANucleoBond ® 96 Xtra EF 3 NEWMACHEREY-NAGELClean-upPCR clean-up and gel extractionNucleoSpin ® Gel and PCR Clean-up 4 UpdatePCR clean-upNucleoSpin ® 8 PCR Clean-up 5 UpdateNucleoSpin ® 96 PCR Clean-up 5 UpdateGenomic DNA clean-upNucleoSpin ® gDNA Clean-up 7 NEWNucleoSpin ® gDNA Clean-up XS 8 NEWRNAMicroRNANucleoSpin ® miRNA Plasma 9 NEWRNA from bloodNucleoSpin ® RNA Blood 10 NEWNucleoSpin ® RNA Blood Midi 11 NEWNucleoSpin ® 8 RNA Blood 12 NEWNucleoSpin ® 96 RNA Blood 12 NEWViral RNA and DNAViral RNA and DNA from serum and plasmaNucleoSpin ® Dx Virus 14 NEWProteinPurification of His-tag proteinsProtino ® 96 Ni-NTA 15 NEWAuxiliary tools and transfer mediaSterile filtrationMN Sterilizer CA 16 Updatewww.mn-net.com

MACHEREY-NAGELProduct informationApart from the Bioanalysis catalog 2011 / 2012 and the new supplement 2013,more product information is outlined in diverse marketing materials.• Product selection guide – summary of the main specifications of all Bioanalysisproducts at a glance• Product guides – overview of certain product groups• Product flyers – presentation of single products• Lab focus flyers – selection of products relevant for certain laboratory focusesFor additional information material, please contact MN (tech-bio@mn-net.com)or see www.mn-net.com/bioflyerscatalog.Constantly updated product information, application data, literature, and productnews are provided at the MN website: www.mn-net.com/Bioanalysis.NEWSMACHEREY-NAGEL introduces the new Bioanalysis icons. Each product chapteris now represented by a new icon. The icons, in addition to the different colors ofthe chapters, allow a fast orientation to easily find the products of interest.The new icons will be shown in product information, product material, and marketingmaterial.Plasmid DNA Clean-up RNAMN qualityGenomic DNA Viral RNA and DNA ProteinHigh throughput Accessories Auxiliary toolsEN ISO certificationMACHEREY-NAGEL has been certified under the EN ISO 9001 norm since1996 and for medical devices under the EN ISO 13485 norm since 2001.Selected products are CE-certified in accordance with EU Directive 98/79/EC.Sophisticated German manufacturing and strict quality control lay the foundationfor high quality standards that our products consistently meet. This quality is ensuredby the corresponding MACHEREY-NAGEL seal present on our products.MACHEREY-NAGELMade inGermanyCertified QualityMACHEREY-NAGELForensic productsConsumables used in forensics need to be specially treated in order to deactivateany PCR-amplifiable DNA. MACHEREY-NAGEL has a stringent and controlledproduction process to avoid any contaminating PCR-detectable background.MN products carrying the forensic quality seal contain plastic materials that areethylene oxide treated to inactivate any DNA.www.mn-net.com1

Transfection-grade plasmid DNAtransfection-gradeendotoxin-freehigh- / low-copy plasmidslarge constructsdesalting toolNEWNucleoBond ® Xtra BACFeaturesThe 2 nd generation of anion exchanger for large construct plasmid DNA• Optimized column design – one prep in approximately 75 min• Column filter included – parallel lysate clearing and loading onto the column• Optimal silica material – yields up to 150 μg• Transfection-grade plasmid DNA using proven anion-exchange technology• LyseControl for visualization of completed lysis / neutralizationProduct at a glanceTechnologyAnion-exchange chromatographyFormatGravity-flow columnsLysate clarificationColumn filtersSample material250–750 mLVector size< 300 kbpTypical yield10–150 μgA 260 /A 280 1.80–1.95Isopropanol precipitation CentrifugationPreparation time75 min/prepBinding capacity150 μgPlasmid DNAApplications• Purification of P1, BACs, PACs, and other large constructs from E. coli• Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection For detailed product information and application data, see www.mn-net.com/plasmidOrdering informationProduct Preps Specification REFNucleoBond ® Xtra BAC 10 NucleoBond ® Xtra BAC Columns740436.10with inserted NucleoBond ® Xtra Column Filters,buffers, RNase A25 as above 740436.25Product accessories Pack of Specification REFNucleoBond ® Xtra Combi Rack 1 for use with NucleoBond ® Xtra Midi, Maxi, BAC, 740415AX 100, 500, 2 000, and 10 000 ColumnsNucleoBond ® Xtra BAC Buffer Set 1 additional lysis buffers (1 L each):740437Buffers RES-BAC, LYS-BAC, NEU-BAC, and RNase AFor separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.2www.mn-net.com

Transfection-grade, endotoxin-free plasmid DNAtransfection-gradeendotoxin-freehigh-copy plasmidsNEWNucleoBond ® 96 Xtra EFFeaturesHigh-throughput isolation of endotoxin-free plasmid DNA in 96-well format• Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step• Processing of up to 5 mL bacterial culture• NucleoBond ® Filter Plate for convenient filtration of bacterial lysates• Processing under vacuum or by centrifugation• Innovative MN Wash Plate minimizes risk of cross-contamination• NucleoBond ® Finalizer Plate to speed up DNA precipitationProduct at a glanceTechnologyAnion-exchange chromatographyFormat96-well platesProcessingManualLysate clarification96-well filter platesSample material1–5 mL E. coli cultureVector size< 15 kbp< 300 kbp (without NucleoBond ® Finalizer Plate)Typical yield2–4 μg (1.5 mL LB / TB in 96-well culture plates)10–50 μg (5 mL LB / TB in glass tubes)A 260 /A 280 1.80–1.95Elution volume100–200 μLPreparation time120 min/plateBinding capacity50 μgApplications• Manual isolation of high-copy plasmids• Typical downstream applications: transfection, cloning, sequencing, PCR, restriction analysisPlasmid DNA For detailed product information and application data, see www.mn-net.com/plasmidOrdering informationProduct Preps Specification REFNucleoBond ® 96 Xtra EF 1 x 96 NucleoBond ® Xtra EF Plate, NucleoBond ® Finalizer 740430.1Plate, NucleoBond ® Filter Plate, Culture Plate, MNWash Plate, Elution Plate U-bottom, Gas-permeableFoil, Self-adhering Foil, buffers, RNase A,4 x 96 as above 740430.4Product accessories Pack of Specification REFNucleoVac Vacuum Manifold 1 740681NucleoVac Vacuum Regulator 1 for controlling of vacuum 740641For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.www.mn-net.com 3

PCR clean-up and gel extractionsingle prepmanual HTPautomated HTPmini spin columnssilica matrixUpdateNucleoSpin ® Gel and PCR Clean-upFormerly known as “NucleoSpin ® Extract II”FeaturesPCR clean-up and gel extraction – the two-in-one kit with optimized recovery and elution volume• Two applications, one kit – one buffer with optimal performance for both applications• High recoveries for fragments down to 50 bp• Minimized elution volume: 15 μL – highly concentrated DNA• Binding buffer with pH indicator• Separate buffers for single stranded DNA or SDS containing samples available• Suitable for all gel buffer systems (e.g., TAE, TBE)Clean-upProduct at a glanceTechnologySilica-membrane technologyFormatMini spin columnsSample material< 400 μL PCR reaction mixture< 400 mg TAE / TBE agarose gelFragment size50 bp–approx. 20 kbpTypical recovery 70–95 %A 260 /A 280 1.8–1.9Elution volume15–30 μLPreparation time10 min/6 prepsBinding capacity25 μgApplications• Purification of PCR products• Extraction of DNA from agarose gels• Support protocol for DNA extraction from polyacrylamide gels• Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from agarosegels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)• Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit,see “Ordering information”)• Typical downstream applications: cloning, sequencing, PCR, restriction analysis For detailed product information and application data, see www.mn-net.com/cleanupOrdering informationProduct Preps Specification REFNucleoSpin ® Gel and PCR Clean-up 10 NucleoSpin ® Gel and PCR Clean-up Columns, 740609.10Collection Tubes (2 mL), buffers50 as above 740609.50250 as above 740609.250Product accessories Pack of Specification REFBuffer NTB 150 mL for clean-up of SDS-containing samples 740595.150Buffer NTC 100 mL for clean-up of single stranded DNA 740654.100For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.4www.mn-net.com

PCR clean-upUpdatesingle prepmanual HTPautomated HTP8-well strips96-well plates96-well systemsNucleoSpin ® 8 / 96 PCR Clean-upNucleoSpin ® 8 / 96 PCR Clean-up Core KitFormerly known as “NucleoSpin ® 8 / 96 Extract II”FeaturesClean-up of PCR products in flexible 8-well strip format and for high throughputin convenient 96-well plate format• Time-saving parallel clean-up of PCR products• Complete removal of primers and primer-dimers• Optimal recovery• Suitable for processing under vacuum and centrifugation• Innovative MN Wash Plate minimizes risk of cross-contamination• Suitable for manual or automated use• NucleoSpin ® 8 / 96 PCR Clean-up Core Kits: kits with basic content focused on automation platforms.Additional accessories can be combined as needed.Product at a glanceNucleoSpin ® 8 PCR Clean-upNucleoSpin ® 8 PCR Clean-up Core KitNucleoSpin ® 96 PCR Clean-upNucleoSpin ® 96 PCR Clean-up Core KitTechnologySilica-membrane technologyFormat 8-well strips 96-well platesProcessingManual or automated, vacuum or centrifugationSample material< 100 μL PCR reaction mixtureFragment size 50 bp–10 kbp 50 bp–10 kbpTypical recovery 75–95 % 75–95 %A 260 /A 280 1.7–1.8 1.7–1.8Elution volume 75–150 μL 75–150 μLPreparation time 30 min/6 strips 45 min/plateBinding capacity 15 μg 15 μgClean-upApplications• Manual or automated purification of PCR products For detailed product information and application data, see www.mn-net.com/cleanupOrdering information, see next pagewww.mn-net.com 5

PCR clean-upsingle prepmanual HTPautomated HTP8-well strips96-well plates96-well systemsOrdering informationProduct Preps Specification REFNucleoSpin ® 8 PCR Clean-up 12 x 8 NucleoSpin ® PCR Clean-up Binding Strips, MN Wash 740668Plate, Rack of Tube Strips, buffers60 x 8 as above 740668.5NucleoSpin ® 8 PCR Clean-up 48 x 8 NucleoSpin ® PCR Clean-up Binding Strips, buffers 740463.4Core KitNucleoSpin ® 96 PCR Clean-up 1 x 96 NucleoSpin ® PCR Clean-up Binding Plate, MN Wash 740658.1Plate, Elution Plate U-bottom, Self-adhering Foil, buffers2 x 96 as above 740658.24 x 96 as above 740658.424 x 96 as above 740658.24NucleoSpin ® 96 PCR Clean-upCore Kit4 x 96 NucleoSpin ® PCR Clean-up Binding Plates, buffers 740464.4Product accessories Pack of Specification REFNucleoVac 96 Vacuum Manifold 1 740681NucleoVac Vacuum Regulator 1 for controlling of vacuum 740641Starter Set A 1 for use of NucleoSpin ® 8-well strips on the NucleoVac 96 740682Vacuum ManifoldStarter Set C 1 for use of NucleoSpin ® 8-well strips under centrifugation 740684For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.Clean-up6www.mn-net.com

Genomic DNA clean-upsingle prepmini spin columnsmini spin columns – XS designNEWNucleoSpin ® gDNA Clean-upFeaturesPost clean-up and concentration of large DNA fragments• Effective post clean-up and concentration of large DNA fragments for successfuldownstream applications• High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp• Concentrate your DNA sample easily and faster compared to microdialysis filtrations units• 15 min/10 preps – time-saving procedureProduct at a glanceTechnologySilica-membrane technologyFormatMini spin columnsSample material< 150 μL aqueous DNA solution (< 25 μg DNA)Fragment size100 bp–approx. 50 kbpTypical recovery 80–90 %A 260 /A 280 1.8–1.9Elution volume50–100 μLPreparation time15 min/10 prepsBinding capacity50 μgApplications• Clean-up and concentration of pre-purified DNA (organic extractions)• Clean-up and concentration of DNA from enzymatic reactions• Not recommended for gel or PCR clean-up! For these applications, please use NucleoSpin ® Gel and PCRClean-up (see page 4)• Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplificationClean-up For detailed product information and application data, see www.mn-net.com/cleanupOrdering informationProduct Preps Specification REFNucleoSpin ® gDNA Clean-up 10 NucleoSpin ® gDNA Clean-up Columns,740230.10Collection Tubes (2 mL), buffers50 as above 740230.50250 as above 740230.250For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.www.mn-net.com 7

Genomic DNA clean-upsingle prepmini spin columnsmini spin columns – XS designNEWNucleoSpin ® gDNA Clean-up XSFeaturesPost clean-up and concentration of small amounts of large DNA fragments• Efficient post clean-up of large DNA fragments for successful downstream applications• Recommended for small DNA quantities extracted from, e.g., forensic samples• Unique XS spin column for low elution volume (> 6 μL) – highly concentrated DNA• Easier and faster than microdialysis filtration units• 20 min/6 preps – time-saving procedureProduct at a glanceTechnologySilica-membrane technologyFormatMini spin columns – XS designSample material< 400 μL aqueous DNA solution (< 2 μg DNA)Fragment size100 bp–approx. 50 kbpTypical recovery 60–70 %A 260 /A 280 1.8–1.9Elution volume6–10 μLPreparation time20 min/6 prepsBinding capacity3 μgClean-upApplications• Clean-up and concentration of pre-purified DNA (organic extractions)• Clean-up and concentration of DNA from enzymatic reactions• Not recommended for gel or PCR clean-up! For these applications, please use NucleoSpin ® Gel and PCRClean-up (see page 4)• Typical downstream applications: PCR, enzymatic reactions, Southern blotting, qPCR, STR amplification For detailed product information and application data, see www.mn-net.com/cleanupOrdering informationProduct Preps Specification REFNucleoSpin ® gDNA Clean-up XS 10 NucleoSpin ® gDNA Clean-up XS Columns, 740904.10Collection Tubes (2 mL), buffers50 as above 740904.50250 as above 740904.250For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.8www.mn-net.com

MicroRNAsingle prepmini spin columnsNEWNucleoSpin ® miRNA PlasmaFeaturesIsolation of small RNA and DNA from plasma and serum• Efficient and selective isolation of small RNA from plasma or serum samples• Superior recovery of small RNA > 15 nt• Optional on-column DNA digest for increased sensitivity in downstream applications• Simple and fast procedure – without phenol / chloroformProduct at a glanceTechnologyFormatSample materialFragment sizeElution volumePreparation timeBinding capacitySilica-membrane technologyMini spin columns300 μL plasma or serum (up to 900 μL with multiple loading)< 1000 nt / bp20–50 μL40 min/10 preps (without rDNase digestion)70 min/10 preps (with rDNase digestion)200 μgApplications*• Isolation of small RNA from plasma or serum for cancer research (e.g., reliable monitoring of clinical parameters)• Typical downstream applications: real-time qRT-PCR, chip hybridization* Patent pending; kits to be used for research purposes only (see page 17) For detailed product information and application data, see www.mn-net.com/RNARNAOrdering informationProduct Preps Specification REFNucleoSpin ® miRNA Plasma 10 NucleoSpin ® miRNA Columns, Collection Tubes (2 mL), 740981.10buffers50 as above 740981.50250 as above 740981.250For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.www.mn-net.com9

RNA from bloodsingle prepmanual HTPautomated HTPmini spin columnsmidi spin columnsNEWNucleoSpin ® RNA BloodDirect blood lysis –no selective erythrocyte lysisFeaturesIsolation of RNA from fresh or frozen whole blood (human or animal)• Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysisat 4 °C – complete processing at room temperature)• Superior RNA yield and quality from up to 400 μL whole blood• Efficient on-column DNA removal for increased sensitivity in downstream applications• Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, andheparin)Product at a glanceTechnologySilica-membrane technologyFormatMini spin columnsSample material< 400 μL whole blood (fresh or frozen)Fragment size> 200 ntTypical yield1–8 μg (400 μL whole blood)*A 260 /A 280 1.9–2.1Elution volume40–120 μLPreparation time55 min/6 prepsBinding capacity200 μg* RNA yield strongly depends on the leukocyte number in each individual blood sample.RNAApplications**• Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin)• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology** Kits to be used for research purposes only (see page 17) For detailed product information and application data, see www.mn-net.com/RNAOrdering informationProduct Preps Specification REFNucleoSpin ® RNA Blood 10 NucleoSpin ® RNA Blood Columns with Collection Tubes, 740200.10Collection Tubes (2 mL), buffers, RNase-free rDNase,Liquid Proteinase K50 as above 740200.50For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.10www.mn-net.com

RNA from bloodsingle prepmanual HTPautomated HTPmini spin columnsmidi spin columnsNEWNucleoSpin ® RNA Blood MidiDirect blood lysis –no selective erythrocyte lysisFeaturesMidi spin kit for the isolation of RNA from fresh or frozen blood (human or animal)• Direct total blood lysis: very simple and convenient procedure (no selective erythrocyte lysisat 4 °C – complete processing at room temperature)• Superior RNA yield and quality from up to 1.3 mL whole blood• Efficient on-column DNA removal for an increased sensitivity in downstream applications• Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, andheparin)Product at a glanceTechnologySilica-membrane technologyFormatMidi spin columnsSample material400–1300 μL whole blood (fresh or frozen)Fragment size> 200 ntTypical yield4–26 μg (1300 μL whole blood)*A 260 /A 280 1.9–2.1Elution volume200–400 μLPreparation time75 min/6 prepsBinding capacity700 μg* RNA yield strongly depends on the leukocyte number in each individual blood sample.Applications**• Isolation of RNA from fresh or frozen whole blood from common blood collection tubes (EDTA, citrate, heparin)• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technologyRNA** Kits to be used for research purposes only (see page 17) For detailed product information and application data, see www.mn-net.com/RNAOrdering informationProduct Preps Specification REFNucleoSpin ® RNA Blood Midi 20 NucleoSpin ® RNA Blood Midi Columns with Collection Tubes, 740210.20Collection Tubes (15 mL), buffers, RNase-free rDNase,Liquid Proteinase KFor separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.www.mn-net.com 11

RNA from bloodNEWsingle prepmanual HTPautomated HTP8-well strips96-well platesNucleoSpin ® 8 / 96 RNA BloodDirect blood lysis –no selective erythrocyte lysisFeaturesIsolation of total RNA from whole blood (human or animal) in flexible 8-well strip formator proven 96-well format• Direct total blood lysis: very simple and convenient procedure (no selective erythrocytelysis at 4 °C – complete processing at room temperature)• Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate,and heparin)• Suitable for fresh and frozen blood samples• rDNase included for efficient removal of genomic DNA• Processing under vacuum or by centrifugation• Suitable for manual and automated processing• Innovative MN Wash Plate minimizes risk of cross-contaminationRNAProduct at a glanceNucleoSpin ® 8 RNA Blood NucleoSpin ® 96 RNA BloodTechnologySilica-membrane technologyFormat 8-well strips 96-well platesProcessingManual or automated, vacuum or centrifugationSample material< 400 μL whole blood (fresh or frozen)Fragment size > 200 nt > 200 ntTypical yield 1–8 μg 400 μL (whole blood)* 1–8 μg 400 μL (whole blood)*A 260 /A 280 1.9–2.1 1.9–2.1Elution volume 50–130 μL 50–130 μLPreparation time 60 min/6 strips 100 min/plateBinding capacity 100 μg 100 μg* RNA yield strongly depends on the leukocyte number in each individual blood sample.Applications**• Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with, for example,EDTA, citrate, or heparin• Typical downstream applications: qRT-PCR, next generation sequencing, blotting, array technology** Kits to be used for research purposes only (see page 17) For detailed product information and application data, see www.mn-net.com/RNA12www.mn-net.com

RNA from bloodsingle prepmanual HTPautomated HTP8-well strips96-well platesOrdering informationProduct Preps Specification REFNucleoSpin ® 8 RNA Blood 12 x 8 NucleoSpin ® RNA Blood Binding Strips, MN Wash Plates, 740220Square-well Blocks, Racks of Tube Strips, Elution PlatesU-bottom, Self-adhering Foil, buffers, RNase-free rDNase,Liquid Proteinase K60 x 8 as above 740220.5NucleoSpin ® 96 RNA Blood 2 x 96 NucleoSpin ® RNA Blood Binding Plates, MN Wash Plates, 740225.2Square-well Blocks, Round-well Blocks Low, ElutionPlates U-bottom, Self-adhering Foil, buffers, RNase-freerDNase, Liquid Proteinase K4 x 96 as above 740225.4Product accessories Pack of Specification REFNucleoVac 96 Vacuum Manifold 1 740681NucleoVac Vacuum Regulator 1 for controlling of vacuum 740641Starter Set A 1 for use of NucleoSpin ® 8-well strips on the NucleoVac 96 740682Vacuum ManifoldStarter Set C 1 for use of NucleoSpin ® 8-well strips under centrifugation 740684For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.RNAwww.mn-net.com 13

Viral RNA and DNA from serum and plasmasingle prepmanual HTPautomated HTPmini spin columnsmini spin columns, CE-markedfunnel columnsNEWNucleoSpin ® Dx VirusCE-IVD marked kitFeaturesCE-IVD marked mini spin kit* for the isolation of viral RNA and DNA• CE-marked in accordance with EU Directive 98/79/EC• Fits into in-vitro diagnostic workflows• Compatible with fresh or frozen human serum and plasma treated with EDTA or citrate• Highly consistent viral RNA and DNA isolation for a broad range of viral titers• Also suitable for animal samples* CE-IVD marked kit: not available in all countries, please inquire.Product at a glanceTechnologyFormatSample materialFragment sizeElution volumePreparation timeBinding capacitySilica-membrane technologyMini spin columns150 μL plasma or serum (fresh or frozen, EDTA or citrate treated)100 bp–approx. 50 kbp50 μL30 min/6 preps40 μgViral RNA and DNAApplications• Isolation of viral RNA from 150 μL human plasma or serum• Isolation of viral DNA from 150 μL human plasma or serum• Isolation of viral RNA and viral DNA from 150 μL human plasma or serum (simultaneous isolation)• Isolation of viral RNA and / or viral DNA from animal samples, swabs, plasma, or serum(out of the scope of the IVD Directive 98/79/EC).• Typical downstream applications: enzymatic amplification and detection of viral RNA and / or viral DNA(e.g., PCR, RT-PCR) For detailed product information and application data, see www.mn-net.com/virusOrdering informationProduct Preps Specification REFNucleoSpin ® Dx Virus 50 NucleoSpin ® Dx Virus Columns with Collection Tubes, Lysis Tubes 740895.50(1.5 mL), Collection Tubes (2 mL), Elution Tubes (1.5 mL), buffers,Proteinase K, Carrier RNAFor separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.14www.mn-net.com

Purification of His-tag proteinsNEWbulk materialsingle prepmanual HTPautomated HTP96-well platesProtino ® 96 Ni-NTAFeaturesHigh-throughput purification of His-tag proteins• Efficient protein purification using chelating group NTA (nitrilotriacetic acid)• Unique Protino ® Purification Plate for leak-free incubation during all steps• Plate outlet and MN Wash Plate prevent cross-contamination• High well-to-well reproducibility• High capacity and high affinity• Purification under native and denaturing conditions• Processing under vacuum or by centrifugationProduct at a glanceTechnologyIMAC (immobilized metal ion affinity chromatography)Chelating ligandNTA (nitrilotriacetic acid)Format96-well plates (Protino ® Purification Plate with special filter frit)Matrix 6 % beaded agarose (cross-linked), precharged with Ni 2+ ,Volume capacity per well1.4 mLRecommended sample volume < 750 μL/well, supplied matrix is sufficient for 96 preps with 50 μLof settled agarose beads per wellBed volumeVariable (50 μL recommended)Binding capacity*< 2 mg per well when using 50 μL of settled agarose* Binding capacity will vary for each polyhistidine-tagged protein and strongly depends on chromatographic conditions.Applications• Purification of polyhistidine-tagged proteins• Batch binding experiments• Screening experiments to optimize chromatographic conditionsProtein For detailed product information and application data, see www.mn-net.com/proteinOrdering informationProduct Preps Specification REFProtino ® 96 Ni-NTA 1 x 96 Protino ® Ni-NTA Agarose, Protino ® Purification Plate, 745425.1MN Wash Plate4 x 96 as above 745425.4Product accessories Pack of Specification REFProtino ® Purification Plate 1 96-well plate, suitable for centrifugation and vacuum 745426.14 as above 745426.4Receiver Plate 10 μm 4 96-well plate with an inserted filter frit of 10 μm pore size, 740989.4suitable for centrifugation and vacuumNucleoVac 96 Vacuum Manifold 1 740681NucleoVac Vacuum Regulator 1 for controlling of vacuum 740641MN Shaker Frame 1 shaking frame for Protino ® Purification Plate 740489For separate kit components, see “Accessories” page 137 of the main catalog Bioanalysis 2011 / 2012.www.mn-net.com 15

Sterile filtrationUpdateauxiliary toolsblottingblood sample storagesterile filtrationdecontamination of EtBrsurface protectionMN Sterilizer CAFeaturesHighest quality syringe filter for fast and efficient sterile filtration of aqueous solutions• Removal of particulates > 0.2 μm• Fast flow of liquid through the filter• Very robust membrane – high stability• Low protein bindingAuxiliary tools and transfer mediaProduct at a glanceFormatSyringe filter unitsHousing materialMethyl methacrylate-butadiene-styrene polymer (MBS)Membrane materialCellulose acetateNominal pore size0.2 μmFilter diameter28 mmEffective filtration area 6.2 cm 2Hold-up volume (after air purge) 150 μLInlet connectionFemale luer lockOutlet connectionMale slip luerMaximum pressure4.5 barMaximum temperature 50 °CSterilization methodPre-sterilized with ethylene oxideApplications• Sterile filtration and removal of particulates from aqueous solutions For detailed product information, see www.mn-net.com/auxiliaryOrdering informationProduct Pack of Specification REFMN Sterilizer CA 50 Disposable filter units, membrane material CA (cellulose acetate),pore size 0.2 μm, membrane diameter 28 mm740401.5016www.mn-net.com

Trademarks • Product use restrictionTrademarks used in this supplementMACHEREY-NAGEL trademarksNucleoBondNucleoSpinProtinokits and cartridges for nucleic acid purification (anion-exchange chromatography)kits for purification of DNA and RNA (silica-membrane technology)kits for purification of recombinant proteins (affinity chromatography)Trademarks of other companiesRegistered trademarksTRIzol Molecular Research Center, Inc.PCR is patented by Hoffmann La RocheDisclaimerAll used names and denotations can be brands, trademarks, or registered labels of their respective owner – also if they do not have aspecial denotation. To mention products and brands is only a kind of information, i.e., it does not offend against trademarks and brandsand can not be seen as a kind of recommendation or assessment. Regarding these products or services we can not grant any guaranteesregarding selection, efficiency, or operation.Product use restriction / WarrantyMACHEREY-NAGEL Bioanalysis products and their single components are intended, developed, designed, and sold FOR RESEARCHPURPOSES ONLY, except, however, any other function of the product being expressly described in original MACHEREY-NAGEL productleaflets.MACHEREY-NAGEL products are intended for GENERAL LABORATORY USE ONLY!MACHEREY-NAGEL products are suited for QUALIFIED PERSONNEL ONLY!MACHEREY-NAGEL products shall in any event only be used wearing adequate PROTECTIVE CLOTHING.For detailed information please refer to the respective Material Safety Data Sheet of the product!MACHEREY-NAGEL products shall exclusively be used in an ADEQUATE TEST ENVIRONMENT.MACHEREY-NAGEL does not assume any responsibility for damages due to improper application of our products in other fields of application.Application on the human body is STRICTLY FORBIDDEN. The respective user is liable for any and all damages resulting from suchapplication.DNA/RNA/PROTEIN purification products of MACHEREY-NAGEL are suitable for IN-VITRO-USES ONLY! ONLY MACHEREY-NAGELproducts specially labeled as IVD are also suitable for IN-VITRO-diagnostic use. Please pay attention to the package of the product. IN-VITRO-diagnostic products are expressly marked as IVD on the packaging.IF THERE IS NO IVD SIGN, THE PRODUCT SHALL NOT BE SUITABLE FOR IN-VITRO-DIAGNOSTIC USE!ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY CLINICAL USE (INCLUDING, BUT NOT LIMITED TODIAGNOSTIC, THERAPEUTIC, AND/OR PROGNOSTIC USE).No claim or representations are intended for its use to identify any specific organism or for clinical use (included, but not limited to diagnostic,prognostic, therapeutic, or blood banking). It is rather in the responsibility of the user or – in any case of resale of the products – inthe responsibility of the reseller to inspect and assure the use of the DNA/RNA/protein purification products of MACHEREY-NAGEL for awell-defined and specific application.MACHEREY-NAGEL shall only be responsible for the product specifications and the performance range of MN products according to thespecifications of in-house quality control, product documentation, and marketing material.For detailed information on product use restriction and warranty please refer to the respective user manuals.Trademarks • Product use restrictionwww.mn-net.com17

DNA, RNA, and protein purificationCatalog supplement 2013Service BioanalysisTechnical Support and Customer Service phone +49 (0) 24 21 969-270e-mail tech-bio@mn-net.comProduct Management phone +49 (0) 24 21 969-271+49 (0) 24 21 969-275e-mail pm-bio@mn-net.comSales and Marketing phone +49 (0) 24 21 969-272+49 (0) 24 21 969-273e-mail sales@mn-net.comBusiness Development phone +49 (0) 24 21 969-272e-mail bdm-bio@mn-net.comYour local distributorKATEN300039 Catalog Supplement en2/15/0/09.2012 PDPrinted in Germanywww.mn-net.comMACHEREY-NAGELEN ISO 9001: 2008CERTIFIEDMACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · GermanyGermanyand international:Switzerland:MACHEREY-NAGEL AGFrance:MACHEREY-NAGEL EURLTel.: +49 24 21 969-0 Tel.: +41 62 388 55 00 Tel.: +33 388 68 22 68Fax: +49 24 21 969-199 Fax: +41 62 388 55 05 Fax: +33 388 51 76 88E-mail: info@mn-net.com E-mail: sales-ch@mn-net.com E-mail: sales-fr@mn-net.comUSA:MACHEREY-NAGEL Inc.Tel.: +1 484 821 0984Fax: +1 484 821 1272E-mail: sales-us@mn-net.com