International Journal of Pharma Research & Review, May 2013; 2(5 ...

International Journal of Pharma Research & Review, May 2013; 2(5):13-17book Yugi vaithiya sinthamani (7).Nociception is the neural processes ofencoding and processing of noxious stimuli(8). It is the afferent activity produced inthe peripheral and central nervous systemby the stimuli that have potential to damagethe tissues (9). To prove scientifically, theefficacy of the drug karunkali ver (Acaciaroot) in Diabetic neuropathy central andperipheral acting analgesic activity has beencarried out.MATERIALS AND METHODSDrugs and chemicalsAcetic acid, and CMC, all from Sigma-AldrichChemicals were the chemicals used. Thestandard drugs aspirin and Pentazocin wasprocured from the local market. All theother chemicals and drugs used were ofanalytical grade.Fig. 1: Root of KarunkaliStock solution preparationAs recommended in SOP, 100 grams finepowder of dried root of karungali ver(Acacia catechu) is added in 200 ml ofwater. Then it was boiled continuously tillthe total volume is concise to 1/4 th as adecoction. The filtered supernatant fluidwas employed for the preclinical study.AnimalsAlbino mice (22–28 g) either sex wereobtained from the animal house of animalhousing facility of department ofpharmacology, Vels University, Chennai.Animals were maintained at standardlaboratory conditions and fed with standardfeeding pellets (Sai durga foods, Bangalore).Prior to treatment, the animals were fastedfor 10 and 12 h respectively (10). However,water was made available ad libitum.(ApprovalnumberXIII/VELS/PCOL/05/2000/CPCSEA/IAEC/08.08.2012)Experimental MethodsAcute toxicity safety StudyAcute oral toxicity test for the KarunkaliverKudineer was carried out as per OECDGuidelines 425 (11). As with othersequential test designs, care was taken toensure that animals are available in theappropriate size and age range for theFig. 2: Prepared Drug of Karunkali VerKudineerentire study. The test substance isadministered in a single dose by gavageusing a stomach tube or a suitableintubation cannula. The fasted body weightof each animal is determined and the dose iscalculated according to the body weight.After the substance has been administered,food was withheld for a further 2 hours inmice. The animals were observedcontinuously for the first 4 h and then eachhour for the next 24 h and at 6 hourlyintervals for the following 48 h afteradministering of the test drug, to observeany death or changes in general behaviorand other physiological activities. Singleanimals are dosed in sequence usually at 48h intervals.However, the time interval between dosingis determined by the onset, duration, andseverity of toxic signs. Treatment of ananimal at the next dose was delayed untilone is confident of survival of thepreviously dosed animal. General behavior,respiratory pattern, cardiovascular signs,motor activities, reflexes, change in skin andfur, mortality and the body weight changeswere monitored daily. The time of onset,intensity, and duration of these signs, if any,was recorded.S Umera et.al, IJPRR 2013; 2(5) 14