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<strong>UHPLC</strong><strong>UHPLC</strong> Method Development1.7μm <strong>Fortis</strong> columns will allow the transfer of methods from traditional HPLC to <strong>UHPLC</strong>, saving both time and solvent. In order to performmethod transfer there are several ‘method development’ calculators available to help in making appropriate changes to column dimension,flow rate and gradient conditions. If done properly the overall method time will reduce but resolution and selectivity of solutes will remainconstant or indeed improve. Download at : www.uhplccolumns.com/<strong>UHPLC</strong>_CalculatorEquivalent <strong>UHPLC</strong> Column - ‘Separating Power’First consideration is the ability to scale themethod down in column dimension, lengthand diameter:– Equivalent <strong>UHPLC</strong> columnIf you can retain equivalent column platecount or ‘separating power’ then it is mucheasier to scale down effectively.– ExampleIf you move from a 5μm 150x4.6mm to a1.7μm 50x2.1mm the equivalent separationshould be achieved but a several foldimprovement in analysis time will beachievedColumnLengthEfficiency of5μm250 22,000Efficiency of3μmEfficiency of1.7μm150 12,700 16,800 26,460100 8,300 10,700 21,00050 4,000 6,000 11,20030 3,200 7,00020 3,000Method Development CalculatorDOWNLOAD AT:www.uhplccolumns.com/<strong>UHPLC</strong>_Calculator
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