1.7Î¼m Fortis UHPLC Columns - dichrom

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UHPLC1.7μm Fortis C18 pH options– pH selectivity for method development– pH stable 1-12– Gives high speed of equilibration1.7μm Fortis C18 will operate across the pH spectrum giving the analyst the ability to optimise the correct pH region for their separation.Quickly equilibrating from formic acid to ammonium acetate through to ammonia allows pH, as a method variable, to be rapidly evaluated.Resolution of compounds can be changed radically by altering pH to optimise separation between compound classes.34Column: 1.7μm Fortis C18 30x2.1mmp/n: F18-020201Gradient: 10 - 50% in 5minFlow:0.4ml/minTemp: 20°CWavelength: 254nm1. Uracil2. Procaine3. Fenuron4. 3-Nitrobenzoic acid12pH 2.2431AcidicBasic2NeutralpH 7.2432pH 11.21
Critical Considerations in UHPLCFully ScalableAll Fortis phases can be scaled from 1.7μmall the way through analytical 3μm and 5μmparticles to prep size without any change inretention profile.1.7μm Fortis C18N = 191,670UHPLC– Improve transfer of methodsBy combining the same surface area, pore sizecharacteristics with the identical bonding youhave the ability to scale up or down, also theconfidence to transfer to another laboratorywithout change in selectivity– IssuesIf a small particle used in UHPLC is not thesame as its larger 3μm and 5μm particle thenchanges in resolution and retention can occur,neither of which are acceptable in methodvalidation.1.7um Fortis C18 will alleviate allthese potential issues, leaving theanalyst confident in method transfer.3μm Fortis C18N = 130,1915μm Fortis C18N = 88,7080 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0MinutesHPLC_Acquity_21nov_011AU1.751.51.251.07.5e-15.0e-12.5e-10.0-2.5e-1-5.0e-10.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40HPLC_X Brige_21nov_0112: Diode Array2.90Range: 1.781.25Competitor1.7μm Hybrid C180.240.731.07Competitor3.5μm Hybrid C181.481.622.022.122.242.332.572.762.802: Diode ArrayRange: 2.1541.0AU7.5e-15.0e-12.5e-10.250.811.191.602.362.252.452.192.692.940.01.73-2.5e-1-5.0e-1Major Pharmaceutical Company DataTime0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40Sensitivity GainsPeak height increases in UHPLC due to therise in efficiency (N) from the smaller particle,but it is also inversely proportional to peakwidth, so symmetrical peaks will lead toincreased sensitivity.– Sharp Peak ShapesAll Fortis phases are designed to give thesharpest possible peak shapes.– High EfficiencyMoving from 3μm to 1.7μm Fortis C18gives a peak height increase of 27% in thisexample. The increase from 5um particles is0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0Minutes- All columns 50x2.1mm
Page 1 and 2: Strength in Technology1.7μm Fortis
Page 3 and 4: 1.7μm Phase Chemistry Selectivity1
Page 5 and 6: ComparisonPressure Comparison 1.7μ
Page 7: 0.00 0.20 0.40 0.60 0.80 1.00 1.20
Page 11 and 12: UHPLC Method DevelopmentScaling a M
Page 13 and 14: UHPLC Sample Filter- Low volume in-
Page 15 and 16: 1.7μm UHPLC part numbersFortis C18

1.7Î¼m Fortis UHPLC Columns - dichrom

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