Original article Association between CD4 CD25 T cells and atopy in ...

J ALLERGY CLIN IMMUNOLVOLUME nnn, NUMBER nnARTICLE IN PRESSJartti et al 5FIG 2. Intracellular FOXP3 expression on CD3 1 CD4 1 CD25 1 cells.PBMCs were stained with CD3/Pacific Blue, CD4/allophycocyanin-7, CD25/phycoerythrin, and CD62L/phycoerythrin-cyanine 5 andthen intracellular-stained with an Alexa Fluor 488 isotype control(A) or Alexa Fluor 488 antihuman FOXP3 (B).FIG 4. Seasonal changes in CD4 1 CD25 high and CD4 1 CD25 int T-cellcounts and total pollen counts. T-cell curves were fitted accordingto each 50 values and pollen curves according to each 10 values.One grain increase per m 3 of air in the 1-month average total pollencount preceding blood draw was associated with 0.10% (95% CI,0.050, 0.15) increase in % CD4 1 CD25 high /CD4 (P < .001).FIG 3. Seasonal changes in % CD4 1 CD25 high /CD4 T cells (A) and %CD4 1 CD25 int /CD4 T cells (B) grouped according to sensitization status(none, white; other than pollen, light gray; pollen, dark gray). Inspring, pollen-sensitized children had a 54% higher CD4 1 CD25 int /CD4 T-cell count than nonsensitized children (95% CI, 7.3, 161) bymultivariate analysis (P 5 .023 for interaction). Sample sizes forwinter, spring, summer, and fall seasons, respectively, are 49, 34,40, and 28.FIG 5. Correlation between % CD4 1 CD25 high /CD4 T cells and suppressivecapacity of CD4 1 CD25 1 T cells. Suppressive ratio was definedas CD4 1 CD25 1 depleted/1:4 CD4 1 CD25 1 enriched PBMCs;larger indices represent greater CD4 1 CD25 1 suppressive capacity.other (P 5 .56), active atopic dermatitis (P 5 .85), or activewheezing (P 5 .88) in univariate analyses (data notshown).Finally, we compared CD4CD25 T-cell phenotype withthe capacity of PBMCs to secrete selected cytokines inresponse to phytohemagglutinin stimulation. The proportionof CD4 1 T cells expressing intermediate amounts ofCD25 correlated positively with IL-5 (r 5 0.35; P 5.0010), IL-10 (r 5 0.29; P 5 .0071), and IL-13 (r 50.42; P