Sunrise C18 Sunrise C18-SAC

Sunrise C18 and C18-SACSilanol Activity Controlled C18 HPLC Column◆ New generation reversed-phase utilized silanol groups■ Silanol group and peak tailingIt is generally said that residual silanol groups ona stationary phase such as C18 (ODS) causesabsorption or peak tailing for a sample.Especially silanol groups near a hydrophobicsite don’t solvate with water completely, so thatthey show high absorption for basic compounds.Its peak shows terribly tailing. Several endcappingtechniques have been developed tosolve these problems for many years.■ Silanol activity control technology1st generation2nd generationPellicular type3rd generationBonded type4th generationHighly endcappedtypeHigh carbon andend-capped type5th generationHybrid typeSilanol ActivityControlTechnology(Post-X2)Sunrise C18SunriseC18-SACChromaNik developed the technique thatdecreased only silanol groups with highabsorption activity to a basic compound andremained effective sailnol groups on thestationary phase. Silanol activity control and noend-capping led the existence of silanol groupswith high hydration which created a new andunique reversed-phase separation modeincluding hydrogen bond and ion-exchangeinteraction. Furthermore, silanol activitycontrolling, then end-capping techniqueimproved a peak shape of a basic compoundexceedingly.OSiOSiOSilanol groupswith highabsorption activitySiHOSiHOSiHSilanol ActivityControlTechnologyOSiSiOSiChangeover tosiloxane bondOSiOSiHSilanol activity control◆ Feature of Sunrise seriesSunrise C18•The“1st Choice” column as a fully end-capped C18 column•Full end-cappingafter silanol activity control•Reducingadsorption of a basic compound extremelyely•A good peak shape for a metal cheleting compoundound•Widely available for general reversed-phaseseparationSunrise C18-SAC•The "2nd Choice" column which takes advantage ofeffective silanol groups interaction•Reducingsilanol groups with high adsorption activityity•The new separation mechanism including hydrogen bondand ion-exchange interaction•Effective for separation of a basic compound and a polarcompound•Different selectivity and improvement of separation withoutchanging a mobile phase■ The elution order of pyridineA) Sunrise C18(end-capped)1C) Sunrise C18-SAC(non end-capped)31(2)B) Conventional no end1Column size: 4.6 X 150mm3 Mobile phase:30/70= CH3OH/H2OSample: 1 = uracil(2) = pyridine3 = phenolend-cappedC183(2)(2)0 5 10 15 20retention time / min