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Screening Varieties for Resistanceto Tomato Yellow Leaf Curl VirusMonica Ozores-Hampton, Phil Stansly,Eric Simonne, Eugene McAvoy , Steven Sargent andJeremy Edwards.

Facts: Tomato Yellow Leaf CurlVirus (TYLCV)‣ Is the worst tomato disease,because can have tremendousimpact in yields.‣ The disease is induced by anumber of begomoviruses,transmitted by the whiteflyBemisia tabaci.‣ Leaf symptoms include chloroticmargins, small leaves which arecupped, thick and rubbery. Themajority (up to 90%) of flowersabscise after infection.

In order to lessen the incidence of the TYLCV(There are no wild species as reservoir forTYLCV during the summer):1. Tomato Free Periods2. Eradication of volunteer3. Peppers whitefly controlNow, if the virus become morestrong another tool is “resistantvarieties”.

The TYLCV resistant genetic materialis coming from Israel a dry weatherconditions.ObjectivesTo evaluate the horticultural performanceof the TYLCV varieties such as yield,fruit qualities and post-harvestcharacteristics under South Florida fieldconditions.

VarietySource2007BHN 745FLA 8576FLA 8579FLA 8580HA 3074 (Inbar)HA 3075 (Ofri)HA 3078TygressFL 47 (control)BHN SeedUFUFUFHazeraHazeraHazeraSeminisSeminisHA 3371 (Shanty)Roma TomatoesHazeraHA 3811HazeraSpring 200710 varietiesSWFREC/IMK

VarietySourceBHN 765BHN 745FLA 8579FLA 8632FLA 8633HA 3074 (Inbar)HA 3075 (Ofri)HA 3091Sak 5421Sak 5443Security 28BHNBHNUFUFUFHazeraHazeraHazeraSakataSakataHarris MoranTygressSeminisSebring (control)SyngentaFL 47 (control)SeminisRoma TomatoesShantyHazeraSpring 200814 varietiesImmokalee FarmSak 5808Mariana (control)SakataSakata

VarietyBHN 765FLA 8577FLA 8578HA 3075 (Ofri)HA 3095HA 3096HM 8845Sak 5230Sak 5443SVR 200TycoonTygressFL 47 (control)Sebring (control)Tygress on FL 47FL 47 on TygressSourceBHN SeedUFUFHazeraHazeraHazeraHarris MoranSakataSakataSeminisHazeraSeminisSeminisSyngentaSeminisSeminisSpring 20092 trials Conventional andOrganics16 varietiesImmokalee Farm

Three HarvestExtra-large (5/6), Large (6/6),Medium (6/7) and un-marketable

Results

Spring 2007 vs. 2008 & 09‣ Whitefly pressure was heavier in Spring 2007than in Spring 2008.‣ In 2007, the average whitefly count was 9.9 ±0.38 (mean ± SE) adult per leaf, as comparedto 0.8 ± 1.04 adult per leaf in 2008 and 09.‣ Therefore, number of TYCLV symptomaticplants was lower in 2008 and 09 than 2007.

Virus symptoms0 0.5 1 1.5 2 2.5 3 3.5 4TygressE604 (UF)FTM 5805 (Sakata)Ofri (Hazera)Inbar (Hazera)Tygress (Seminis)FTM 5230 (Sakata)MT 386 (Takii)E603 (UF)E602 (UF)BHN 765 (BHN)E601 (UF)FTM 5443 (Sakata)Tygress inoculatedSecurity 28 (Harris-Moran)Florida 47 (Seminis)

VarietiesSpring 2007FirstHarvestMarketable Yield three Harvest(boxes/acre)XL XL Cull MarketableBHN 745 630cd 770ab 1,433a 1,073bcdFLA 8576 442cd 584ab 1,095bc 985cdFLA 8579 694cd 888ab 1,022bc 1,312bcdFLA 8580 590cd 813ab 694d 1,442bcHA 3074 (Inbar) 991ab 1,472a 833cd 2,015aHA 3075 (Ofri) 726bc 1,025a 708d 1,561abHA 3078 1,118a 1,590a 1,094bc 2,069aTygress 722bc 1,039a 1,146b 1,518abcFlorida 47 (control) 330d 430b 596d 854dSig. ** ** ** **

VarietySpring 2008FirstHarvestMarketable Yield three Harvest(boxes/acre)XL XL Culls Marketable TotalBHN 745 957ab 1,593ab 1,092ab 2,706BHN 765 972ab 1,580ab 1,151ab 2,752FLA 8579 624cd 889d 657cde 2,373FLA 8632 141e 171e 557def 2,185FLA 8633 464d 848d 842bcd 2,286HA 3074 (Inbar) 782abc 1,091cd 516def 2,202HA 3075 (Ofri) 972ab 1,365abc 681cde 2,532HA 3091 1,027ab 1,635a 1,332a 2,444Sak 5421 884abc 1,249bc 1,320a 2,432Sak 5443 823abc 1,571ab 1,010abc 2,613Security 28 1,059a 1,667a 991abc 2,389Tygress 1,019ab 1,262bc 231f 2,580Sebring 721bcd 1,279abc 487def 2,880FL 47 (control) 922abc 1,627a 448ef 2,761Sig. ** ** ** ns

VarietySpring 2009First HarvestMarketable Yield Three Harvest(Boxes/acre)XL XL Culls MarketableBHN 765 1,031c 1,457bcd 2,524a 2,675abcFLA 8577575def863efg 2,012abc 2,148cdeFLA 8578 336f 462g 2,007abc 1,549eHA 3075 (Ofri) 390f 757efg 1,725abcd 2,339bcdHA 3095 457ef 801efg 1,578bcd 1,945deHA 3096 684cdef 1,070def 2,222ab 2,022deHM 8845 965c 1,417bcd 1,301cd 2,810abSak 5230 520ef 663fg 2,130ab 1,639eSak 5443 812cde 1,106def 1,695bcd 2,509bcdSVR 200 1,369b 1,876ab 928d 2,763abcTycoon 922cd 1,233cde 1,634bcd 2,144cdeTygress 1,733a 2,165a 1,023d 2,903abTygress on FL 47 1,422ab 1,920ab 928d 3,162aFL 47 on Tygress 963c 1,697abc 1,232cd 3,173aFL 47 (control) 985c 1,568bcd 1,140d 2,947abSebring (control) 407f 1,247cde 1,098d 3,210aSig. ** ** ** **

Yield (Boxes/acre)2,5002,0001,5001,0005000Organic Marketable YieldSpring 20096/76/65/6Varieties

RomasSpring 2007Varieties First harvest Culls Marketable Yield(Boxes/acre)Shanty 600 1,300 951HA 3811 159 314 622Sig. * * *

RomasSpring 2008VarietySpring 2008First HarvestMarketable Yield Three Harvest(Boxes/acre)XL XL CullsMarketableYieldShanty 504 757ab 2,181a 1,682bSak 5808 337 655b 298b 3,021aMariana(control)774 1,086a 394b 2,884aSig. ns * ** *

facts: Diseases &Post-Harvest Evaluation

There are TYLCV varieties more susceptible tobacterial spot than other varietiesMost of TYLCV varieties tested in 2008 and 09were susceptible to Fusarium crown rotThe most firm fruits were the lowest color rating.1. Spring 2007, Tygress very firm, but had lowcolor rating.2. Spring 2008, the firmness fruit Sec 28, but hadthe lowest color rating. The most firm andhighest color rating among the roma varietieswas Shanty.3. Spring 2009, there were no differences in colorand firmness, pH, TTA , pH and soluble solidscontent among TYLCV varieties content in thesecond harvest, except for few varieties.

Variety Blind Evaluation Spring200828 participants(scale 1-5; 1= very poor and 5 = very good)Participant perception under a blind test ratedbest overall ‘Security 28’ and ‘Sak 5443’.VarietiesOverall RatingRound TomatoesBHN 745 3.2bBHN 7653.1bcFLA 85792.4deFLA 8632 1.8fFLA 86332.2efHA 30912.7cdHA 3074 (Inbar) 3.0bcHA 3075 (Ofri) 3.2bcSak 54213.1bcSak 5443 3.7aSecurity 28 3.8aTygress2.9bcSebring (control) 3.1bcFL 47 (control) 3.2bSig. **Roma TomatoesShanty 2.2bSak 5808 2.6bMariana (control) 3.6a

Variety Blind EvaluationSpring 200923 participants(scale 1-5; 1= very poor and 5 = verygood)Participant perception under a blind test ratedbest overall ‘Tygress’, ‘SVR 200’,‘HM 8845’ and BHNVariety Overall RatingBHN 765 3.6bFLA 85772.8cdFLA 85782.3efHA 3075 (Ofri)2.3efHA 30952.5defHA 3096 2.2fHM 88453.7abSak 5230 3.0cSak 5443 3.1cSVR 2003.7abTycoon2.6deTygress 4.0aFL 47 (control) 3.1cSebring (control)2.9cdSig **

2006Low VirusPressure2007High VirusPressure2008Low VirusPressure2009Low VirusPressureBest :HA 3075 (Hazera), S-50257, VT-60774, andVT-60780 (ZeraimGedera).Best:Hazera 3078, 3074(Inbar) and 3075(Ofri).Roma: ShantyBest:Tygress and 3074(Inbar).Roma: Sakata 5808Best:‘Tygress’, ‘Tygresson FL47’ graftedplants and ‘SVR200’ (Seminis).Good:BHN 745 (BHN Seed)and Tygress (Seminis) .Good:Tygress (Seminis)Good:Security 28 (HarrisMoran), BHN 745 and765, Hazera 3091 and3075 (Ofri), and Sakata5443.Good:BHN 765, HarrisMoran 8845,

http://swfrec.ifas.ufl.edu/veghort/trials/tylcv/

For providing monetary or in-kindsupport to this project.Thanks….thanks…Thanks‣ Southwest Vegetable GrowersAssociation and Florida TomatoCommittee.‣ Sakata, Hazera, Harris Moran, andBHN.‣ Redi Plants and Six’L Farms.