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Experiment Procedures - Frederiksen

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2EDVO-Kit # 275AIDS Kit II: Simulation of HIV-1 Detection by Western BlotTable of ContentsPage<strong>Experiment</strong> Components 3<strong>Experiment</strong> Requirements 3Background Information 4<strong>Experiment</strong> <strong>Procedures</strong><strong>Experiment</strong> Overview 8SDS Protein-Agarose Gel Electrophoresis 9Capillary Western Blot Analysis 12Molecular Weight Estimation 16Study Questions 18Instructor's GuidelinesNotes to the Instructor 19Pre-Lab Preparations 20Avoiding Common Pitfalls 23Idealized Schematic of Results 24Study Questions and Answers 25Material Safety Data Sheets 26EVT 004185AMThe Biotechnology Education Company ® • 1-800-EDVOTEK • www.edvotek.com

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