Experiment Procedures - Frederiksen

22EDVO-Kit # 275AIDS Kit II: Simulation of HIV-1 Detection by Western BlotPre-Lab PreparationsInstructor's GuideEACH LAB GROUP SHOULD RECEIVE THE FOLLOWING:First Day• Boiled Components A-E (pre-aliquoted) and Component F• Components to prepare agarose gel(see Student Experimental Procedure)• Practice gel loading solution (optional)• 20-30 ml 95-100% methanol• Diluted Tris-Glycine-SDS electrophoresis buffer• 80 ml Diluted transfer buffer• 1 Western Blot membrane• 3 Filter paper pieces• Paper towels and plastic wrap• Small non-acrylic boxes for soaking membranes and gels• 20-30 ml Distilled water• Pipet• 400 ml Beaker (weight for blot)Second Day• Western Blot Stain, diluted• Destaining solutionDuplication of this document, in conjunction with use of accompanying reagents, is permitted for classroom/laboratory use only. This document, or any part, may not be reproduced or distributed for any other purposewithout the written consent of EDVOTEK, Inc. Copyright © 2005 EDVOTEK, Inc., all rights reserved.EVT 004185AMThe Biotechnology Education Company ® • 1-800-EDVOTEK • www.edvotek.com