Cytoculture Cytotoxic Safety Cabinet - Esco

Touchpad data entry buttons permit controlsettings and access to diagnostics, default settingsand hierarchical menus.Color coded indicator lamps display greenfor primary function (fan operation); bluefor secondary function (fluorescent lightsand electrical outlet); and orange forcaution (UV lamp ON).Programmable automatic UVlight timer simplifies operation,enhances contamination control,extends UV lamp life and savesenergy.A graphical interface indicates cabinet performance.Digital read-out with alpha-numeric display indicates allinput, status and alarm functions.All functions can be user activated through touch-padprogramming access; see Operations Manual.Sentinel Microprocessor Control System, ProgrammableWhen programmed ON • the start-up sequence confirms status with Air Safe and local time display.• the Personal Identification Number (PIN) access restricts unauthorized adjustments.• an airflow alarm warns of deviations from normal velocities.With a range of specialist featuresincorporated into our Cytoculture CytotoxicSafety Cabinets (CYT), Esco brings youthe ideal solution to your cytotoxic drugpreparation challenges. Our CYT cabinetsare designed to:• Maintain the highest level of cleanlinesswithin the work zone ensuring the bestpossible level of patient safety andproduct cleanliness.• Provide the highest level of operatorsafety to protect you and your personnelfrom hazardous compounds used incytotoxic drugs.• Incorporate an advanced range ofadditional safety features, includinga specialized Tri-Filter design, toguarantee the highest possible levelsof environmental, operator and productprotection. Contaminated air from thework zone is immediately filtered bya HEPA filter below the work zone tominimize the possibility of airbornecontamination.• Meet the requirements of all relevantsafety standards for handling cytotoxicdrugs (see technical specifications fordetails).• Provide ISO Class 3 air cleanliness withinthe work zone.With an additional HEPA filter placeddirectly below the work zone, filters onour CYT cabinets can be changed withoutexposing the ambient environment andservice personnel to potential hazards.This unique design allows contaminatedfilters to be removed easily, and all contaminatedareas to be sterilized withoutcompromising safety.Designed and Built forEnhanced UsabilityAll Esco Cytotoxic Safety Cabinets areergonomically designed for a high level ofusability and efficiency. They incorporatea number of features to ensure operatorcomfort and enhanced productivity.• The ergonomic angled front improvesreach into the work zone and preventsglare off the sliding sash.• The aesthetically pleasing stainless steelinterior will never rust, chip or generateparticles, enhancing operator andproduct protection.• The interior back and side walls arefabricated from a single piece of stainlesssteel, with no joints or sharp corners.This makes the cabinet exceptionallyeasy to clean.• Built-in warm white, electronically ballasted,5000K lighting provides excellentillumination of the work zone to reduceoperator fatigue. The reliable lightingsystem is zero-flicker and instant start.• The front sliding sash window ismotorized for movement, facilitatingeasy transfer of reagents and equipmentinto and out of the work zone.• Spacious knee room is provided for theoperator in a sitting position, ensuringcomfort during use.• The easily removable multi-piece stainlesssteel work surface constructionincreases cleanability.Enhanced Filtration SystemThe enhanced filtration system is designedto provide the highest level of air qualitywithin the work zone.• Esco CYT cabinets provide ISO Class 3air cleanliness within the work zone (asper ISO 14644.1), significantly “cleaner”than the usual Class 5 classification oncabinets offered by the competition.• A highly specialized Tri-Filter designincorporates two standard ULPA filtersand an additional V-bank ULPA exhaustfilter, providing a world class level of aircleanliness to protect the operator, theproduct and the environment.Mini-pleat Separatorless Filter (left) vs.Conventional Aluminium Separator Filter (right)Esco cabinets use Swedish Camfil Farr ® mini-pleatfilters without aluminum separators to increase filterefficiency, minimize the chance of leakage, and toprolong filter life. Filters include a lightweightaluminum frame for structural stability andelimination of swelling common to conventionalwood frames.3

Cytoculture Cytotoxic Safety Cabinet AirflowExhaust ULPA filterSupply ULPA filterDynamic air barrier, inflow and forwarddirected downflow air convergeSecondary exhaust HEPA filterULPA-filtered airUnfiltered / potentially contaminated airRoom air / Inflow airAir enters the cabinet through perforationslocated along the front of the work zonebefore mixing with used downflow airin a common chamber below the workzone (this inflow air does not mix withthe filtered downflow air in the cabinetsmain chamber). The mixed air then passesthrough the HEPA filter located beneaththe work zone.The HEPA filtered air then passes throughinternal ducting in the back wall of thecabinet to a common air plenum where35% is exhausted through the ULPAexhaust filter and 65% is forced evenlythrough the ULPA supply filter. Thissterilized air then passes through themain chamber as downflow air, flushingall contaminates from the work zone.At the work surface the downflowairstream splits and enters the common airchamber beneath the work zone throughperforations located at the front and backof the main chamber, from where thecycle is repeated.4• Advanced minipleat separatorless HEPAand ULPA filter technology maximizessurface area. This energy efficient designreduces operating costs, extends filterlife, and delivers increased laminar airflowuniformity, ensuring better productprotection than conventional filters.• The high quality ULPA filters (perIEST-RP-CC001.3) operate at typicalefficiency of >99.999% at 0.1 to 0.3micron sizes, providing superior productprotection.• For enhanced safety the first exhaustfilter can be changed easily withouttime consuming decontamination.• An integral metal filter guard protectsthe ULPA filters from accidentaldamage.• Unique U-channels in work surfaceconstruction protect the main filtersfrom liquid spills and small parts.User-Friendly Control SystemThe user-friendly Esco Sentinel microprocessor-basedcontrol system, fitted toour CYT cabinets, supervises the operationof all cabinet functions. The controlsare easily configurable to meet yourrequirements and comes equipped with anumber of enhanced features to promotecabinet usability and safety.• All critical cabinet airflow parameterscan be monitored using the built-inaccurate true airflow velocity sensingtechnology. Temperature compensatedsensors ensure increased accuracy.• Audible and visual alarms ensureproduct protection by alerting the userin the event of low airflow.• Password-protected administrationcan be set to restrict access to the mainmenu ensuring that the cabinet cannotbe operated by unauthorized personnel.• Built-in solid state variable speed controllers,with integral RFI and noise filters,are superior to conventional “step”controllers and offer infinite adjustmentfrom zero to maximum setting.Cabinet ConstructionAll Esco products are manufactured to thehighest quality, using the finest materials,for the most demanding laboratoryapplications.• All components are designed formaximum chemical resistance andenhanced durability for a long servicelife, making Esco’s CYT cabinets oneof the most cost effective solutions onthe market.• The main body of the cabinet is constructedof industrial-grade electrogalvanized steelfor durability and enhanced safety.Antimicrobial Powder-CoatingBacterial Population (10 folds)8642All exterior painted surfaces are powder-coated withEsco Isocide, an antimicrobial inhibitor to diminishcontamination. Isocide is integrated into the coatingsubstrate and cannot wash out or diminished byrepeated cleaning. Performance results are availableupon request. Contact Esco or your Esco SalesRepresentative for details.0 4 hours 8 hours 12 hours 16 hours 20 hours 24 hoursContact Time in HoursEsco Specialty Products • Cytotoxic Safety Cabinets

Model CYT Cytotoxic Safety Cabinet Technical Specifications17213141516891011475 mm (18.7")203 mm (8")MAX. OPENINGACCESSOPENING670 mm (26.4")34562190 mm (86.2")12KNEE SPACE DEPTH760 mm (30.0")245 mm (9.6")1270 / 1870 mm(50" / 73.6")1420 / 2030 mm(60" / 80")750 mm (29.5")780 mm (30.7")1. Exhaust ULPA filter2. Blower3. Downflow ULPA filter4. Standard UV light Retrofit Kit provision5. Standard IV bar Retrofit Kit provision6. Electrical outlet Retrofit Kit provision(two single outlets in workzone)7. Electrical and electronics panel8. Fluorescent lamp9. Plugged service fixture provisions(2 on each side)10. Stainless steel multi-piece work tray11. Stainless steel armrest12. Exhaust ULPA filter13. Esco Sentinel microprocessorcontrol system14. Motorized laminated glass slidingsash window15. Single-piece stainless steel back walland side walls16. Removable side panel for plumbingaccess5GeneralRequirementsAir Quality Filtration Electrical SafetyStandardsComplianceEN 12469, EuropeISO 14644.1, Class 3, WorldwideIEST-G-CC1001, WorldwideIEST-G-CC1002, WorldwideEN-1822 (H14), EuropeIEST-RP-CC001.3, USAIEST-RP-CC007.1, USAIEST-RP-CC034.1, USAUL-C-61010A-1, USACSA C22.2, No.1010-92, CanadaEN-61010-1, EuropeIEC61010-1, International• All cabinet components are cleanroomcompatible.• The external structure of all our cabinetsis coated with Esco Isocide antimicrobialcoating to protect against contaminationand inhibit bacterial growth. Isocideeliminates 99.9% of surface bacteriawithin 24 hours of exposure to furtherensure the cleanliness of the work zone.Blower Efficiency• Esco Cytotoxic Safety Cabinets incorporatepermanently lubricated direct drivecentrifugal blowers. The energy efficientexternal rotor motor design reducesoperating costs and has extremely lowvibration.• The blower system automaticallymaintains airflow as the filters becomeloaded, ensuring optimum efficiencyand product protection without theneed for constant manual adjustment.Designed and Built to ExceedSafety CriteriaAt Esco safety is of paramount importance.We take extra care in the design andconstruction of all cabinets to ensure allcomponents used in our products meet orexceed all applicable safety requirements.• Each cabinet is individually factorytested for safety and performance inaccordance with international standards.Each unit is shipped with a documentationoutlining the tests undertakenand the units individual results for eachcabinet.• All electrical components are UL listedor UL recognized, ensuring superiorelectrical safety for the operator.WarrantyEsco Cytotoxic Safety cabinets includean extended 3 year warranty, excludingconsumable parts and accessories. Contactyour local Sales Representative for specificwarranty details.

General Specifications, Cytotoxic Safety CabinetsNote to customer: Insert electrical voltage number into last model number digit _ when ordering.Model CYT-4A_ CYT-6A_Nominal Size 1.2 meters (4') 1.8 meters (6')External Dimensions(W x D x H)Internal Work Area, Dimensions(W x D x H)1420 x 780 x 2190 mm55.9" x 30.7" x 86.2"1270 x 603 x 670 mm50.0" x 23.7" x 26.4"2030 x 780 x 2190 mm79.9" x 30.7" x 86.2"1870 x 603 x 670 mm73.6" x 23.7" x 26.4"Internal Work Area, Space 0.60 m 2 (6.4 sq.ft.) 0.90 m 2 (9.7 sq.ft.)Average AirflowVelocityInflowDownflow0.45 m/s (90 fpm)0.30 m/s (60 fpm)Exhaust Volumewith ThimbleDuctULPA Filter Typical EfficiencyCBV Exhaust Volume 611 m 3 / h (360 cfm) 931 m 3 / h (548 cfm)Static Pressure at CBVExhaust Volume39 Pa / 0.15 in H 2O 63 Pa / 0.25 in H 2O>99.999% for particle size between 0.1 to 0.2 micronsSound Emission Per EN 12469* 61 dBA 63 dBAFluorescent Light Intensity At Zero Ambient >1300 Lux (>121 foot candles) >1200 Lux (>111 foot candles)Cabinet Construction1.5 mm (0.06") 16 gauge electrogalvanized steel with Isocide white oven-baked epoxy powder-coatingNet Weight 383 kg (844 lbs) 500 kg (1102 lbs)Shipping Weight 403.6 kg (889.8 lbs) 476.5 kg (1050.5 lbs)Shipping Dimensions, Maximum (W x D x H)1570 x 940 x 2210 mm61.8" x 37.0" x 87.0"2150 x 950 x 2200 mm84.6" x 37.4" x 86.6"Shipping Volume, Maximum 3.26 m 3 (115 cu.ft) 4.49 m 3 (158.6 cu.ft)ModelVoltage6Electrical**CYT-4A1, CYT-6A1CYT-4A2, CYT-6A2220-240 VAC, 50Hz, 1 phase110-120 VAC, 60Hz, 1 phaseCYT-4A3, CYT-6A3220-240 VAC, 60Hz, 1 phase* Noise as measured in open field / anechoic chamber.** Additional voltages may be available; contact Esco for ordering information.Customized to Meet YourSpecific NeedsEsco CYT cabinets can be configured tomeet unique needs including:• Lead shielding for nuclear medicineapplications.• An optional carbon filter impregnatedwith KI (potassium iodide) in place of theadditional HEPA filter (this configurationdoes not protect service technicianswhen changing contaminated filters).Esco Centrifugal Fan with External Rotor Motor (left) vs.Conventional Fan with Standard Motor (right)Esco cabinets use German made ebm-papst ®permanently lubricated, centrifugal motor/blowers with external rotor designs.Integrated blades narrow the profile andeliminate need for a motor shaft.Motors are selected for energy efficiency,compact design, and flat profile. Thecompletely integrated assembly optimizesmotor cooling.All rotating parts are unitized and balancedfor smooth, quiet, vibration-free operation.Comprehensive Performance Testing At EscoEvery Cytoculture CYT model manufactured by Esco isindividually tested, documented by serial number andvalidated with the following test methods.Inflow / downflow velocityPAO aerosol challenge for filter integrityAirflow pattern visualizationElectrical safety to IEC61010-1Additional KI-Discus containment and microbiologicaltesting is performed on statistical sampling basis.Esco Specialty Products • Cytotoxic Safety Cabinets

Purchase SpecificationsCYT Cytoculture Cytotoxic Safety CabinetGeneral Performance and Certifications1. The cytotoxic safety cabinet shall comply toEN 12469 as Class II Microbiological SafetyCabinet.2. The cabinet shall protect: (a) the operator andlaboratory environment from hazardous drugcompounds used in the work zone; (b) the productand process within the work zone from airbornecontamination from ambient air; (c) andthe product and process within the work zonefrom cross contamination.3. The retention efficiency for the front apertureshall be not less than 99.999% Microbiologicaltesting for cabinet performance shall beperformed on a statistical sampling basis.4. Each 220-240V, AC, 50Hz model shall be listed byCE for electrical safety.5. Original documentation specific to each cabinetserial number shall be provided with thecabinet and maintained in the manufacturers’records. Test data verifying all performancecriteria shall be available upon request toinclude: (a) inflow velocity through directinflow measurement method; (b) downflowvelocity and uniformity; (c) filter leak scan withaerosol challenge for all three filters; (d) electricalsafety.Filtration System6. The cabinet shall employ a triple-filter designwith one supply downflow filter and two exhaustfilters. Both downflow and all exhaust ULPA filtersshall be ULPA-type per IEST-RP-CC001.3 or H14per EN 12469 with filter media complying withEN 1822 fire retardant performance.7. The secondary exhaust filter shall be V-Bankdesign ULPA-type per IEST-RP-CC001.3 or H14per EN 12469 with media complying with EN1822 fire retardant performance.8. The filters shall be within an aluminum frame withmini-pleat design without aluminum separators;no wood or fiberboard shall be used in the filterassembly.9. Typical ULPA filter efficiency shall be more than99.999% for particle size between 0.1 to 0.3microns.10. An integral filter guard shall be affixed to preventdamage to the filter media.11. The filters shall be (a) individually tested bythe manufacturer, (b) individually tested afterassembly, and (b) easily accessible for scan testingin situ by means of a dedicated upstream samplingport accessible from within the cabinet.12. The supply filter shall be angled and orientedto the 10° cabinet front angle to maximizedownflow uniformity over the work surface.13. A removable, perforated metal diffuser shallbe installed below the supply filter to optimizeairflow uniformity and to protect from damage.14. The secondary exhaust HEPA filter shall beinstalled beneath the work zone surface.15. The secondary exhaust HEPA filter fittingshall be designed for easy and safe filterchangingoperations without the need for priordecontamination of the cabinet.Blower System16. The cabinet shall have a direct drive, permanentlylubricated centrifugal blower/motor dynamicallybalanced in two planes compliant to ISO 2710for low noise, low vibration and long filter life.17. The blower/motor shall have an external rotordesign and include an automatic thermal cut-outto disable the motor in case of overheating.18. The blower/motor shall have an semi automaticability to compensate for filter loading.19. The blower/motor system shall be enclosed withina dynamic chamber shaped steel plenum andintegrated with the removable supply filterassembly to simplify filter changing.Cabinet Design, Construction, Cleaning20. The cabinet shall be of triple wall design wherebyall positive pressure plenums capable of handlingcontaminated air shall be surrounded by negativepressure. No positive pressure areas shall beaccessible external to the cabinet. The third wall shallconceal utilities.21. The cabinet shall maintain containmentperformance even when removable work areacomponents are removed for cleaning.22. The work tray shall be multi-piece, removable,stainless steel.23. The closed sidewall shall be sealed withoutperforations, return air slots or concealed areaswhich can contain contaminants.24. The cabinet shall be free of sharp edges,nonfunctional protrusions, bolts, screws orhardware, and all metal edges shall be deburred.25. The cabinet exterior top shall be slanted todiscourage placement of foreign objects and tomaintain proper exhaust airflow.Ergonomics and Convenience26. The front sash shall be frameless to maximizevisibility, and accessible for cleaning front andback. Sash glass shall be laminated safety glass tomaintain containment in the event of accidentalbreakage.27. The front sash movement shall be motorizedand the movement shall be controlled from thecabinet’s microprocessor control panel.28. Magnetic, not mechanical, proximity sensors shallwork in conjunction with the control system toindicate proper sash position for containment.29. Fluorescent lamps shall be mounted behind thecontrol panel module out of the work zone.Electronic ballasts shall be used to eliminateflicker, extend lamp life and reduce heat output.30. The UV lamp, if installed, shall be mounted behindthe control panel and away from the user’s line ofsite for protection.31. The UV lamp shall operate via an automatic timerwith automatic shut-off managed by the microprocessorcontroller and shall be interlockedwith the blower/motor and fluorescent lightsfor safety.32. The cabinet shall be designed with a 10° angledfront to optimize user comfort, reduce glare andmaximize reach into the work area.33. The arm rest shall be raised above the front airgrille to prevent airflow blockage and improvecomfort.34. Penetrations for petcocks and service fittingsshall be provided; penetrations shall be offset toimprove user access.35. The cabinet shall provide a 245 mm (9.6") kneespace inward for sitting operators.36. The cabinet shall be freestanding on castorwheels with leveling rod supports.Control and Alarm System37. All cabinet functions shall be managed by aprogrammable microprocessor control systemcapable of software updates via Internetdownloads.38. The microprocessor controller shall be mountedon the main control panel facing down towardthe user.39. The controller shall include soft-touch keypadcontrols and backlit LCD displays to permitoperation of the blower/motor, light, UV lamp,electrical outlet(s) and menu.40. The controller shall be user programmable insitu to enable or disable functions such as PIN(personal identification number) access restriction,cabinet start-up protocol, airflow alarm and othermicroprocessor controlled operations outlined inthe user manual.41. When programmed ON, the start-up protocolshall perform an automatic pre-purge and postpurgecycle to ensure proper cabinet operation.42. The controller shall include a blower/motor hoursmeter to display aggregate motor running timeto assist in predictive maintenance.43. Audible and visual alarms shall be provided forunsafe conditions such as improper airflow orsash position.44. Airflow shall be monitored by a temperaturecompensating, thermistor-based, true air velocitysensor mounted in the cabinet.45. The airflow display and alarm system shall beindividually calibrated before shipment.46. The main control panel shall exhibit continuousdisplay of air velocity and a 24-hour clockdisplay.Certification, Service and Decontamination47. The first exhaust ULPA filter design shall allow forthe filter to be changed while the cabinet bloweris still activated to protect service personnel.48. The cabinet shall be approved for both hydrogenperoxide vapor (HPV) and formaldehydedecontamination protocol, before the removalof secondary exhaust HEPA filter.49. All panels leading to potentially contaminatedand/or hazardous areas shall be color coded red(with the exception of the first exhaust ULPA filterdirectly below the work zone surface).50. All components with the exception of blower/ motor and ULPA filters shall be locatedoutside of contaminated air spaces to facilitateservicing without the need to decontaminate thecabinet.51. All exterior surfaces shall be painted with apermanent antimicrobial inhibitor coating tominimize contamination.7

8Biological Safety ProductsContainment / Pharma ProductsDuctless Fume Hoods / Carbon FiltrationGeneral Purpose Scientific EquipmentIndustrial Lab EquipmentIn-Vitro Fertilization ProductsPCR ProductsPharmacy ProductsLab Animal Research ProductsLab Thermostatics ProductsLab Ventilation / Chemical Fume Products / Lab FurniturePowder Handling ProductsSince 1978, Esco has emerged as a leader in the development of controlled environment, laboratory and pharmaceuticalequipment solutions. Products sold in more than 100 countries include biological safety cabinets, compounding pharmacyequipment, containment / pharma products, ductless fume hoods, in vitro fertilization workstations, lab animal researchproducts, laboratory fume hoods, laboratory ovens and incubators, laminar flow clean benches and PCR products andinstrumentation. With the most extensive product line in the industry, Esco has passed more tests, in more languages,for more certifications, throughout more countries than any biosafety cabinet manufacturer in the world. Esco remainsdedicated to delivering innovative solutions for the clinical, life science, research and industrial laboratory community.www.escoglobal.com.Biological Safety Cabinets • Cytotoxic Safety Cabinets • Laminar Flow Clean Benches • Pharmacy IsolatorsEsco Technologies, Inc. • 2940 Turnpike Drive, Units 15-16 • Hatboro, PA 19040, USAToll-Free USA and Canada 877-479-3726 • Tel 215-441-9661 • Fax 215-441-9660www.escoglobal.com • usa@escoglobal.comEsco Micro Pte. Ltd. • 21 Changi South Street 1 • Singapore 486 777Tel +65 6542 0833 • Fax +65 6542 6920 • mail@escoglobal.comwww.escoglobal.comEsco Global Offices | Beijing, China | Kuala Lumpur, Malaysia | Manama, Bahrain | Guangzhou, China |Hanoi, Vietnam | Melaka, Malaysia | Mumbai, India | Philadelphia, PA, USA | Salisbury, UK | Shanghai, China | Seoul,Korea | Delhi, India | Osaka, Japan | Manila, Philippines | Midrand, South Africa | Jakarta, Indonesia | Singapore9010031_Compounding Equipment_CYT_Brochure_A4_vB1_02/12Esco can accept no responsibility for possible errors in catalogues, brochures and other printed materials. Esco reserves the right to alter itsproducts and specifications without notice. All trademarks and logotypes in this material are the property of Esco and the respective companies.escoglobal.comLIVE SUPPORTEsco Micro Pte LtdCert. No: 651076PT Esco Bintan IndonesiaCert. No: 651333/E