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Small-molecule MALDIQuantification of acetylcholine und choline in brain dialysate(Coop. Inst. of Pharmacology, Univ. of Frankfurt)N +OON +OH• HPLC not needed• directly from physiological salt conc.• standard dried-droplet preparation• making use of the higher rep rate of the 4800MALDI-TOF(TOF)• accumulation of 2000 laser shots• lower required sample amounts(0.5 µL) allowed us to measuretime-resolved
Results 50 fmol/µL ACh_ 48005 pmol/µL Ch% Intensity10080212.0367146.0593846.8146.1172Acetylcholin: 146.11768.9E+4[Matrix-CO 2 +H] + : 146.060060234.0198Choline40IS2IS1104.1070178.1338228.0123249.99722038.9637113.1627172.0395190.050023.0683 160.1338265.9758379.1466010.0 88.4 166.8 245.2 323.6 402.0Mass (m/z)25
Page 1 and 2: From Small-Molecule Drugs to Proteo
Page 5 and 6: Relative AbundanceTandemMSorMS/MSM
Page 8 and 9: 5000400030002000conferenceuntil 200
Page 10 and 11: MALDI- Matrix AssistedLaser Desorpt
Page 12: nevertheless a difficult start
Page 16: can an exact protein molecular mass
Page 20 and 21: % Intensity% IntensityNative MALDI
Page 22 and 23: ESI and today also MALDI solved an
Page 26: Analyte/IS*ConcAnalyte/IS*Conc10.90
Page 32 and 33: in order to analyze a proteome key
Page 34: 1-the conventional way :"MALDI pept
Page 37: Protein Identifizierung : ESI or MA
Page 40 and 41: in order to analyze a proteome key
Page 44 and 45: Remaining problems :• possibly lo
Page 46 and 47: (self)critical discussion about pro
Page 48 and 49: ut with large efforts and investmen
Page 51: the alternative.....back to hypothe
Page 55 and 56: A new tool MALDI imaging mass spect
Page 57: AK KarasU. Bahr, Ph.DB. Meyer, Ph.D

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