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TABLE OF CONTENTSINTRODUCTION3 Customer Service and Technical Support3 How to Place an Order4 Factory Repairs and Limited Warranty5 Instrument Service PlansINSTRUMENTS, HARDWARE,REACTORS & FLOW CONDITIONERS7 Pinnacle PCX Derivatization Instrument10 Vector PCX Derivatization Instrument12 CRX400 Post-column Reactors12 Flow Conditioner13 Flow Restrictors13 Glyphosate & AMPA Clean-upCHROMATOGRAMS,COLUMNS & GUARDS15 Columns & Guards Catalog Information16 Cation-exchange Columns & GuardsCatalog Information17 Reversed-Phase Columns & GuardsCatalog Information17 Specialty Columns18 Cation-exchange GARD ColumnProtection System18 Lithium Amino Acids25 Sodium Amino Acids30 Carbamate Pesticide34 Glyphosate Herbicide Analysis Column35 Alkion Column39 Paralytic Shelfi sh Toxins40 Mycotox Mycotoxin43 Polyether Antibiotics44 Analysis of Voglibose inPharmaceutical Formulations45 Anion-exchange Column For Nitrite/Nitrate & Bromate Analysis46 Analysis of Bromate in Drinking Water47 Analysis of Alendronic Acid inPharmaceutical FormulationsREAGENTS & CHEMICALS49 TRIONE Ninhydrin Reagent50 o-Phthaladehyde50 Naphthalene-2,3-Dicarboxaldehyde50 Vanillin51 p-Dimethylaminobenzaldehyde (DMAB)51 Thiofluor51 o-Phthalaldehyde Diluents52 Solutions for Sample Preparation forAmino Acid Analysis (SERAPREP andURIPREP)52 Hydrolysis Reagent for CarbamatePesticide Analysis CB13052 Hydrolysis Reagent for CarbamatePesticide Analysis CB130.253 Hypochlorite Diluent for GlyphosateHerbicide Analysis and Amino Acids53 ChlorAC54 Restore54 Pure Water54 Extraction Solution54 Samples and Standards Diluents(Li220 & Na220)55 Calibration Standards and Test Mixtures57 Artifi cial PerspirationELUANTS58 Sodium-based Solutions for AminoAcid Analysis58 Lithium-based Solutions for AminoAcid Analysis58 Glyphosate Herbicide Analysis58 Potassium-Based Solutions forALKION Column for AminoglycosideAntibiotics, Paraquat/Diquat in WaterAnalysis & Streptomycin58 Biogenic Amines/Polyamines Analysis58 Muti-Residue Mycotoxin AnalysisAPPLICATION KITS59 30-min High-Effi ciencyCollagen Hydrolysate59 30-min High-Efficiency Protein Hydrolysate59 Standard 60-min Protein Hydrolysate59 High-Efficiency 55-min Protein Hydrolysate59 High-Effi ciency 55-minCollagen Hydrolysate59 60-min Oxidized Feed Hydrolysate60 70-min High-Efficiency PhysiologicFluid/Native Sample60 Standard 185-min PhysiologicFluid/Native Sample60 High-Effi ciency 120-min PhysiologicFluid/Native Sample60 Temperature Gradient, PhysiologicFluid/Native Sample Kit60 High-Effi ciency 7-min PKUDetection/Physiologic Fluid61 Carbamate Pesticides,23+ Compounds61 Carbamate Pesticides, EPAMethod 531.161 Carbamate Pesticides, AOACmethod 985.2361 Carbamate Pesticides, AOACmethod 531.261 Glyphosate Herbicide, EPA Method547, AOAC Method 991.0861 Biogenic Amines/Polyamines61 Aminoglycoside Antibiotics61 Paraquat/Diquat in Water61 Multi-Residue Mycotoxin61 Afl atoxins61 Polyether Antibiotics61 Paralytic Shellfi sh ToxinsBECKMAN 6300/730062 Analytical Columns and Guards62 Eluants62 Reagents and ChemicalsREPLACEMENT COMPONENTS,FITTINGS & TUBING63 Pinnacle PCX Flow Diagram–Two-Pump System64 Pinnacle PCX Flow Diagram–One-Pump System65 Vector PCX Flow Diagram66 PCX5200 Flow Diagram67 Pinnacle PCX Reagent Pump andValve Components67 Vector PCX Reagent Pump Components67 PCX5200 Reagent Pumps withPiston Wash67 PCX5200 Reagent Pump Components67 Pinnacle PCX ReplacementReactor Cartridge68 Vector PCX Substitute ReactorVolumes or Replacement Reators68 PCX5200 Substitute ReactorVolumes or Replacement Reactor68 Panels for PCX520068 Reagent Reservoirs & Cap Assemblies68 Pressure Regulators, Valves & Guages69 Flow Restrictors69 Filters & Frits69 Tees & Unions69 Tubings, Plastic70 Tubing, Type 316 Stainless Steel70 Fittings, Plastic70 Fittings, Type 316 Stainless Steel71 Fuses & Miscellaneous AccessoriesLCTECH / GPC PRODUCT LINE72 The LCTech Story73 GPC Ultra76 EVA78 GPC Vario82 GPC Quattro80 GPC UNO84 Gel Permeation Columns85 GPC Product Numbers/Description

INTRODUCTIONLCTECH / MYCOTOXIN PRODUCT LINE87 UVE89 AcceClean90 Sample Clean-up Columns92 Elisa Test Kits94 Mycotoxin Product Numbers/Description94 Histamine Analysis Kits94 Elufi x95 Eluvac95 Mineral Oil Hydrocarbon Index(H53-Method) Elufi x ColumnsMETHOD ABSTRACTS97 Afl atoxins (110)98 Multi-Residue Mycotoxin Analysis (203.1)100 Multi-Residue Mycotoxin Analysisof Dry Grains (208)102 Analysis of Alendronic Acid inPharmaceutical Formulations (328)103 30-Min Amino Acid Analysis ofHydrolyzed Samples (380)104 70-Min Amino Acid Analysis ofPhysiological Samples (382)105 PKU and MSUD (107)106 Aminoglycoside Antibiotics in Feed (205)108 Analysis of Biogenic Amines (102)110 Bromate Analysis (120)112 Post-column LC Analysis of23 N-Methyl Carbamates (108)114 Expanded HPLC Method forN-Methyl Carbamates (112)116 Analysis of Formaldehyde (325)117 Glyphosate and AMPA Analysis inCrops (206)119 Simultaneous Determination of Nitrite &Nitrate in Processed Foods (123)122 Paralytic Shellfi sh Toxins (105.2)124 Polyether Antibiotics (104)125 Paraquat and Diquat in Water (117)126 Sulfonamide Artifi cial Sweetners inFood Products (124)127 Analysis of Voglibose in PharmaceuticalFormulations (327)130 General Terms and Conditions of Sale131 DistributorsCUSTOMER SERVICE& TECHNICAL SUPPORTPickering Laboratories has earnedthe reputation for strong TechnicalSupport in HPLC post-column technology:chemistry, columns, reagents,and derivatization instruments. Westart with assisting our customers inidentifying the best HPLC congurationrequired for a successful integrationof our HPLC post-column system.Then we continue our supportthrough and beyond the warrantyperiod to ensure our customers’ chromatogramsreect current standardsof quality and reproducibility.HOW TO CONTACT USFor Customer Service or TechnicalSupport contact:sales@pickeringlabs.comsupport@pickeringlabs.com800-654-3330 / 650-694-6700Fax: 650-968-0749HOW TO PLACE AN ORDERPlease contact us directly toplace an order:orders@pickeringlabs.com800-654-3330 / 650-694-6700Fax: 650-968-0749Pickering Laboratories, Inc.1280 Space Park WayMountain View, CA 94043When ordering, please providethe following:• Complete shipping and billingaddresses• Catalog number and quantityof each item• Brief product description, as foundin Ordering Information• Purchase order number (and contractnumber, if applicable)• Name and telephone number ofperson calling• Conrmation required• Special instructions, e.g., package markings,mode of transit if other than UPS• If ordering a complete post-columnsystem, please specify the brandof HPLC with which it will beoperating. We will advise you ifany modications to the HPLC arenecessary.From European Countries, PleaseContact our European Distributor:LCTech GmbHPostfach/P.O. Box 1360D-84403 Dorfen, GermanyTelefon: +49(0)-80 81-93 68-0Telefax: +49(0)-80 81-93 68-10email: info@lctech.deInternet: www.lctech.deFrom non-European countries, pleasecontact Pickering Laboratories directly.We will provide the names of companieswhich distribute our products.TERMS• A minimum order is not required• Net 30 days for established accounts,or prepayment in U.S. dollars,C.O.D., or Letter-of-Credit• We accept MasterCard & VISA,Discover Card or American Express• Prices are Ex-Works Seller’s Factory,Mountain View, CaliforniaU.S. Destinations & Canada:Freight charges are prepaid andadded to the invoice. Shipments arevia UPS-Ground unless otherwisespecied.Destinations Outside of the U.S.:New Accounts: Before a credit-linehas been established, new accountorders must be pre-paid. Freightcharges are collect.Established Accounts in Good Standing:Freight charges are collect. Unless otherwisespecied, shipments overseaswill be via consolidated air-freight tokeep the costs as low as possible.Prices and specications are subjectto change without prior notice.03PRODUCT CATALOGPICKERING LABORATORIES10/11

INTRODUCTIONINSTALLATION & TRAININGWe encourage you to take advantageof Pickering Laboratories’ individualizedtwo or three-day installationand training courses. For an all-inclusivefee we can provide post-columnsystem installation and training atyour site by an experienced chemistfrom Pickering Laboratories or it’srepresentatives. The training includesperforming analyses, routine maintenanceand troubleshooting, andchromatogram interpretation.Some of our authorized distributorsoffer their own training and installationservices.Whenever you buy installation andtraining by Pickering Laboratories,please call Technical Support earlyto schedule our visit to your site. Thisis especially important if you buyinstallation through a distributor orHPLC Company because we need tohear all the details directly from you.Individually tailored courses are alsogiven at our facility in MountainView, California. Please telephone orfax with your requirements and wewill advise cost and schedule.PRODUCT RETURNSProducts can be returned if unopened.A restocking fee of 15 % oflist price will be applied. Before returninga product for any reason, please:• Phone or fax requesting a ReturnedGoods Authorization Number.• Provide a Purchase Order Numberfor product returns intended forservice and repair.• Package the product so that it willarrive undamaged. Use the originalpacking if possible. If in doubt,please call. Pickering Laboratoriescannot assume responsibility forgoods which arrive damaged inshipment.• Return the completed Health &Safety Declaration with the itemsintended for service or repair (Seepage 4 for details.)FACTORY REPAIRSPickering offers factory repair for it’sinstruments (PCX Model 5200, PinnaclePCX and Vector PCX only).We will re-build or replace componentsas necessary in our judgment,then test the instrument to makecertain that the original problem hasbeen solved.For repairs, we will charge for shipping,parts and labor (current shop rate).What is Required:Obtain a Return Goods AuthorizationNumber (RGA) and a Healthand Safety Declaration form. Whenthe instrument is returned it musthave the RGA noted on the outsideof the box and a completed Healthand Safety Declaration, with theinstrument. To expedite your repairwe request the instrument is returnedwith a purchase order for the cost toevaluate the system (current shop rate).What is Done:Upon arrival we will evaluate yoursystem to generate an estimated costfor repairs. Once we have your approvalwe will complete the repair.We will replace damaged parts, pressuretest the instrument, and thentest the instrument to conrm thesystem meets the products originalmanufacturer’s specication.What you get:The minimum parts replaced are:Restrictor(s), Seal(s) and Filters. If wetest the instrument to determine thatit meets the original manufacturer’sspecication, we will warranty theinstrument for 1 year on all the partsthat were replaced. If you requestthat we do not complete this test wewill not warranty our work.FIELD SERVICEBecause of our limited personnel, weprefer to resolve problems withoutsite visits, but when that just is notenough, we will schedule a site visitwith you. Please call for a quotation.On-site service entails an initialevaluation on the rst day, withrepairs potentially extending to thesecond day to allow for shipment ofparts. The shipment of parts will beincluded in the invoice.On-site repairs costs will includetravel time, air fare, hotel, car rentals,expenses, plus parts and labor.COLUMN REPACKING SERVICEIf you have determined or suspectthat the performance of yourPickering cation-exchange columnor guard has degraded, consider ourRepacking Service before purchasinga new column. Columns eligible forrepacking include the Pickering aminoacid, glyphosate and polyaminecolumns and their respective guards.If you are not certain that thecolumn or guard must be repacked,Pickering will test it for a service fee,freight or postage prepaid. This feewill be credited to any consequentrepacking cost if tests indicate thatrepacking is needed.For this repacking service, Pickeringwill charge 75 % of the current listprice of that particular column orguard. If the column hardware can’t bere-used, Pickering Labs will provide anew column at 85 % of list price.Repacked and tested columns andguards carry the same warranty asnew columns and guards.Analytical columns and guards willbe repacked only one time.LIMITED WARRANTYInstrumentsPickering Laboratories, Inc.,(Pickering) Instruments are warrantedto be free of defects in materialand workmanship under normalinstallation, use, and maintenance,for a period of one year from thedate of delivery to the customer.Pickering will replace or repair,without cost, any defective items. Expendableitems such as check valves,pistons, piston seals, and lters areexcluded from this warranty. In addition,physical damage, poor-quality

INTRODUCTIONreagent- and sample-induced damage,and instrument damage due tocustomer’s misuse are not covered bythis warranty.Analytical ColumnsPickering’s Analytical Columns arewarranted for 90 days to be free ofdefects in materials and workmanshipunder normal installation, use, andmaintenance, for the warranted timebeginning from the date of deliveryto the customer. Pickering will replacethe Analytical Column under warrantyif found defective in material orworkmanship. However, the warrantyis void if the Analytical Column wasdamaged due to customer’s misuse.How to Obtain Warranty ServiceIf there is a problem with yourInstrument or Analytical Columnwithin the warranty period, immediatelynotify Pickering or yourlocal authorized representative. Ifthe Instrument or Analytical Columnwas not purchased directlyfrom Pickering, please contact thevendor where it was purchased. AnyInstrument, part of the Instrument,or Analytical Column returned toPickering for examination or repairshall have Pickering’s prior approval(call for a Returned Goods AuthorizationNumber) and be sent prepaidby the Customer. Return transportationwill be at Pickering’s expense ifthe Instrument, part of the Instrument,or Analytical Column is foundto be defective and under warranty.HEALTH & SAFETY DECLARATIONInstruments and columns must bedecontaminated and cleaned priorto returning to Pickering. To protectand ensure the safety of Pickeringpersonnel, customer must properlyush instrument of any and all hazards(e.g. reactive chemicals, bloodborne pathogens). Please contactPickering to obtain a Health andSafety Declaration Form (B-0107)describing return instructions andcustomer responsibilities. Failure todo so will result in delay of serviceand/or additional charges.INSTRUMENT & SERVICE PLANSIntroducing the Service Plan tomatch the quality and reliability youhave come to expect from PickeringLaboratories. These offerings providefactory trained engineers experiencedin HPLC and post-columntechnologies to service your Pickeringpost-column derivatization instruments.Each plan has the features andbenets most requested by customersto offer the best value and effectivenessfor today’s busy laboratories.When you sign-up for the PickeringLaboratories Service Plans you areassuring the continued quality andreliability of your post-column instrumentas well as your analysis.BENEFITS OF SERVICE AGREEMENTS• Free Software Upgrades• Installation of all required performanceupgrades• PM/Service performed by PickeringLaboratory trained service specialists• Fixed Annual Cost – No Surprises• Unlimited Technical/Analytical Support• Multi-Unit Service Discounts Available05COMPARISON OF SERVICE PLANSFEATURES FACTORY WARRANTY WARRANTY UPGRADE DEPOT SERVICE ELITE SERVICE*Telephone support to isolateand resolve technical problemsMonday - Friday8AM - 5PMExcluding HolidaysMonday - Friday8AM - 5PMExcluding HolidaysMonday - Friday8AM - 5PMExcluding HolidaysMonday - Friday8AM - 5PMExcluding HolidaysRecertification Not included Included Included IncludedSite visits At prevailing rates Two (including PM) At prevailing rates Two (including PM)Preventative Maintenance At prevailing rates One One OneFreight Charges Not Included Included Included IncludedParts and Labor Included Included Included IncludedService Plans offered for the Pickering Laboratories PCX5200, Pinnacle PCX and Vector PCX post-column derivatization instruments.Pre-service inspection is required for instruments that have an expired manufacturers warranty or service contracts.*The Service plan provides a total of two service calls that will cover parts, labor and travel for the period of one year. Included is unlimitedtelephone support from Pickering Technical Service Engineers and Applications Specialists.For more information and quotations,please contact Pickering Laboratories’ headquarters Technical Supportat support@pickeringlabs.com or 800-654-3330.PRODUCT CATALOGPICKERING LABORATORIES10/11

“A LOT HAS CHANGED SINCE PRODUCING OUR FIRST HPLC POST-COLUMNDERIVATIZATION SYSTEM, BUT OUR GOALS HAVE REMAINED THE SAME; PROVIDETOP-NOTCH SYSTEMS WITH THE GREATEST FLEXIBILITY AND QUALITY.”We have optimized our instruments for post-column applications as well as ease of use, ruggedness and efciency.MICHAEL V. PICKERING, PH.D. / FOUNDERSAREETA NERKAR / RESEARCH CHEMISTChalk talk: giving words vision.

INSTRUMENTS & HARDWAREPINNACLE PCX DERIVATIZATION INSTRUMENTPart of the complete integrated system of instruments, chemicals, columns, methods and support fromPickering Laboratories.The Pinnacle PCX Sigma Series is an optimized HPLC post-column derivatization system for analysis of Amino Acids,Carbamates, Mycotoxins, Antibiotics and many other applications. This instrument is the culmination of PickeringLaboratories’ 30 years of experience in post-column analysis instrument manufacturing. Each component is specicallydesigned to optimize the sensitivity and selectivity of post-column analysis.Only Pickering Laboratories offers the complete package of chemicals, columns, methods and post-column systems.Because each part of the method is designed to work together, Pickering Laboratories can offer the extraordinarypromise that the analysis is guaranteed to work for the intended application.The Pinnacle PCX reects the ease of use, reliability and ruggedness you have come to expect fromPickering Laboratories.07SYSTEM DESIGN ADVANCEMENTS RESULT IN OPTIMIZED ANALYSIS:• The electronic syringe pump provides true pulse-free flow for superior sensitivity and consistency. The pumpcylinder and head is made from a single piece of inert ceramic for durability and non-reactivity.• Electronic valves eliminate troublesome check valves and allow automated pump flushing.• The quick-change reactor cartridge makes application switching easy and replacements quick and inexpensive.• The column oven utilizes circulating air for consistency of heating and quick cooling.• Inert flow path extends system life and reduces maintenance.• The PCX control software allows for precise control of the reagent delivery and conservation.• Column oven temperature gradient programming improves separation and run times. Pinnacle PCX is the onlypost-column system with this feature.• Works with any HPLC system.• RoHS compliantPRODUCT CATALOGPICKERING LABORATORIES10/11

INSTRUMENTS & HARDWAREFEATURE HIGHLIGHTThe Pinnacle PCX has a column oven capable of programmable temperature gradients. This feature is useful in AminoAcid Analysis by enabling additional exibility when optimizing methods. Using the temperature gradient program allowsfor improved separation, shortened analysis time and ne-tuned methods for specic compound detection.The Chromatogram in Figure 1 demonstrates some of the clear advantages of the temperature gradient program. Comparedto the standard method without the temperature gradient the total run time is 42 % shorter. Additionally, separationis improved.With the temperature gradient program, you are able to focus on different areas of the chromatogram for your speciccompound of interest.124457 8 111092023151716o1870 C21o65 C241925223045363537393840FIGURE 1High-efficiency Lithiumfor Physiological samplesusing temperaturegradient26 2728 2931332344334 oC461214:313341420 10 20 30 40 50 60 70 min1 Phosphoserine2 Taurine3 Phosphoethanolamine4 Urea5 Aspartic acid6 Hydroxyproline7 Threonine8 Serine9 Asparagine10 Glutamic acid11 Glutamine12 Sarcosine13 -Aminoadipic acid14 Proline15 Glycine16 Alanine17 Citrulline18 -Amino-n-butyric acid19 Valine20 Cystine21 Methionine22 Allo-Isoleucine23 Cystathionine*Internal Standard24 Isoleucine25 Leucine26 Tyrosine27 Phenylalanine28 -Alanine29 -Amino-i-butyric acid30 Homocystine31 -Aminobutyric acid32 Tryptophan33 Ethanolamine34 Ammonia35 Hydroxylysines36 Ornitine37 Lysine38 1-Methylhistidine39 Histidine40 3-Methylhistidine41 Anserine42 Carnosine43 Arginine44 Glucosaminic Acid*45 2-Aminoethyl-cysteine (AEC)*PINNACLE PCX SPECIFICATIONS• Dimensions: (h x w x d): 54 x 26.7 x 46.4 cm(21.25 x 10.5 x 18.25 inches)• Weight: 60 lbs (27.2 Kg) – Duplex• Electrical: 100-120 V, 50/60 Hz, 1.7 A, 200 W or 200-240 V,50/60 Hz, 0.8 A, 200 W• Mains voltage ± 10 % of nominal• Installation (over voltage) category II, pollution degree 2• Indoor use only• Environmental: Altitude up to 6500 ft• Ambient Temperature 15 °- 25 °C• Relative humidity up to 80 % at 31 °CREAGENT PUMPS• True pulse-free syringe pump• Single piece ceramic barrel• Completely inert flow path• Maximum operating pressure 500 psi• Programmable flow rate

INSTRUMENTS & HARDWARE• Flow range: 50 µL to 1500 µL/minute• Refill cycle of 60 seconds• Automatic piston wash• Automatic reagent flush cycle• No check valvesCONTROL• Computer controlled• Windows PC with 2000, NT, XP• USB connection or networkREACTOR• Heated reactor for temperature from 5 °C above ambientto 130 °C• Easy replacement reactor coil cartridges• Range of reactor dwell volumes; 0.1 mL to 3 mL• Reaction coil withstands up to 42 bar (600 psi) inletpressure at 130 °C• Thermal safety switch limits temperature to 150 °C toprevent damage• Fast responseSAFEGUARDS• In-line check valve: Prevents reagent back flow into the columnwhen HPLC pressure drops• Post-column system over pressure: A pre-calibrated relief valveopens at 35 bar (500 psi) to prevent rupture of the post-columnreactor tubing in the event of down-stream blockage• Back-pressure regulator: Applies 7 bar (100 psi) to the detectorflow cell outlet (waste) to prevent detector noise andprecipitation due to out-gassing or boilingCOLUMN HEATER AND REACTOR CONTROLLER• Heater accepts up to 6 or 8 mm OD(0.25 or 0.31 inch) x 50-250 mm in length (column and guard)• Programmable temperature gradient• Power cooling capability• Temperature holds within ± 0.4 °C from the set point. Can be setwith 1 °C resolution from 5 °C above ambient to 75 °C• Easy column accessINSTRUMENT PACKAGE AND FLOW PATH• Advanced fluidics valve management system• Easy access to internal components• Standard fittings• Post-column pressure relief valve• Side panels easily removed for service access• Integrated reagent reservoir tray• Corrosion proof pan and panelsDISPLAY• Backlit LCD• Real time temperature and pressure display• System status icons• Simple system control interfaceGAS PRESSURE MANIFOLD AND REGULATOR• Panel mount manifold• Regulator maintains 0.3 bar (3-5 psi) on reagentreservoirs with 3-5 bar (45-75 psi) source pressure• Safety pressure relief valve opens at 1 bar (14 psi)• Manifold has two 1/4-28 tubing connections• Gas lines with anti-syphon valvesPRESSURIZED REAGENT RESERVOIR• One liter capacity (2 and 5 L reservoirs available)• Maintained under inert gas pressure to inhibitoxidation of oxygen sensitive reagents• Valve built into reservoir cap permits spargingduring reagent preparation• Reagent reservoirs fitted with 3.1 mm (1/8”)OD, oxygen-impermeable SARAN tubing foroxygen-sensitive reagents09PINNACLE PCX CATALOG INFORMATIONCATALOG NO. APPLICATION REAGENT PUMP REACTOR VOLUME VOLTAGE1153-10111153-10121153-10211153-10221153-10311153-10321153-10411153-10421153-10511153-10521153-10911153-10921153-10611153-10621153-10711153-10721153-10811153-10821153-11011153-1102Amino Acids (OPA detection), Aminoglycoside Antibiotics, Biogenic Amines,Polyamines, Paraquat/DiquatAmino Acids (TRIONE® Ninhydrin detection), Ochratoxin A, Formaldehyde,BromateSingle 0.15 mL 120 V240 VSingle 0.5 mL 120 V240 VMycotoxins including Aflatoxins & Fumonisins Single Knitted1.4 mLCholesterol Single Knitted2.8 mL120 V240 V120 V240 VCarbamate Pesticides, Glyphosate Herbicide, Streptomycin, (OPA detection) Dual 0.5 mL 120 V240 VMicro System for Carbamate Pesticides & Glyphosate Herbicide Dual 0.1 mL 120 V240 VParalytic Shellfish ToxinsOshima MethodDual 2.0 mL 120 V240 VTrichothecene Mycotoxins Dual Knitted1.2 & 1.6 mLPolyether Antibiotics Dual Knitted1.4 mLVoglibose Analysis Single Knitted1.4 mLNOTE: Columns and chemicals are sold separately120 V240 V120 V240 V120 V240 VPRODUCT CATALOGPICKERING LABORATORIES10/11

INSTRUMENTS & HARDWARE• Indoor use only• Environmental: altitude up to 6,500 ft• Ambient temperature 15 º - 25 ºC• Relative humidity up to 80 % at 31 ºCVECTOR PCX DERIVATIZATION INSTRUMENTThe Vector PCX instrument is the newest addition to thePickering family of post-column derivatization systems.Introduced a year after the Pinnacle PCX launch, the newVector PCX serves as another post-column choice ideal forapplication-specic methods.Vector PCX provides the selectivity and sensitivity requiredfor most standard post-column applications while beingreliable and easy to use. Since Vector PCX does not have acolumn oven it is important to use the HPLC column ovento ensure stable column temperature and prevent retentiontime drifts and separation problems.Designed as a step-up from our previous model PCX5200,the Vector PCX has several improvements and innovations.VECTOR PCX FEATURES• Inert pumps• PEEK liquid manifolds• Integrated gas manifold• Easy access and monitoring of fluidics• Simple control interface (no computer required)• Reduced bench space profileMatched with Pickering’s Chromatographic Grade ® reagents,eluants and columns, the Vector PCX instrument is a solidchoice for laboratories’ standard post-column needs.VECTOR PCX SPECIFICATIONS• Dimensions: (h x w x d): 43 x 21.6 x 41.2 cm(17 x 8.75 x 16 inches)• Weight: 11.5 kg (25.3 lb.)• Electrical: 100 - 120 V, 50/60 Hz 1.7 A, 200 W or200-240 V, 50/60 Hz, 0.8 A, 200 W• Mains voltage ± 10 % of nominal• Installation (over voltage) category II, pollution degree 2REAGENT PUMPS• Independently adjustable, low-pulsation• Adjustable from 0.05 to 2.00 mL/minute against back-pressuresof up to 2000 psi• Flow Accuracy 3 % for flowrates of 0.33 ml/min and above, 0.01ml/min for flowrates below 0.33 ml/min• Flow Precision 0.5 % RSD• Sapphire pistons• Liquid ends, including check valve housing, PEEK• PEEK bypass/purge valves for each pump located on front ofinstrument panel• Automatic piston washFLOW PATH• Independent pressure transducer for each pump 210 bar(0-3000 psi)• Diamond-packed restrictors, matched to flow rate and viscosityof reagents• PEEK bypass/purge valves• Replaceable reagent filter• PEEK mixing manifoldREACTOR• Heater reactor controls at ±0.4 ˚C for temperatures from 5 ˚Cabove ambient to 130 ˚C• Range of reactor dwell volumes, 0.1 mL to 3 mL• Reaction coil withstands up to 42 bar (600 psi) inlet pressureat 130 ˚C• LCD display of actual temperature or set point• Thermal safety switch limits temperature to 150 ˚CGAS PRESSURE MANIFOLD AND REGULATOR• Panel mounted manifold• Regulator maintains 0.3 bar (3-5 psi) on reagent reservoirs with3-5 bar (45-75 psi) source pressure• Safety pressure relief valve opens at 1 bar (14 psi)• Manifold has two 1/4-28 tubing connections• Gas line with anti-siphon valvePRESSURIZED REAGENT RESERVOIR• One liter capacity (2 and 5 L reservoirs available)• Maintained under inert gas pressure to inhibit oxidation of oxygensensitivereagents• Valve built into reservoir cap permits sparging during reagentpreparation• Reagent reservoirs fitted with 3.1 mm (1/8’’) OD, oxygen-impermeableSARAN tubing for oxygen-sensitive reagents and/or with3.1 mm (1/8’’) OD FEP tubing.

INSTRUMENTS & HARDWARESAFEGUARDS• A pressure switch installed between LC (eluant) pump and sampleinjector turns off power to reagent pumps and reactor when theeluant pump pressure drops to 35 bar (500 psi), ensuring thatreagent will not flow upstream and damage the analytical column.Low eluant pressure can result from power failure, eluant pumpmalfunciton, automatic or intentional shut-down, or an emptyreservoir. The Vector PCX will not restart automatically.• Post-column system over pressure: A pre-calibrated relief valveopens at 35 bar (500 psi) to prevent rupture of the post-columnreactor tubing in the event of down-stream blockage• Back-pressure regulator: Applies 7 bar (100 psi) to the detectorflow cell outlet (waste) to prevent detector noise and precipitationdue to out-gassing or boilingVECTOR PCX CATALOG INFORMATIONCATALOG NO. APPLICATION REAGENT PUMP REACTOR VOLUME VOLTAGE1154-40511154-40521154-40011154-40021154-40611154-40621154-40711154-40721154-40811154-40821154-41011154-4102Carbamate Pesticides, Glyphosate Herbicide, Streptomycin, (OPA detection) Dual 0.5 mL 120 V240 VStreptomycin Dual 0.5 mL 120 V240 VParalytic Shellfish Toxins – Oshima Method Dual Knitted2.0 mLTrichothecene Mycotoxins Dual Knitted1.2 mL and 1.6 mLPolyether AntibioticsInert PEEKDualKnitted1.4 mLVoglibose Analysis Single Knitted1.4 mLNOTE: Please contact Pickering for assistance in confi guring a custom Vector PCX that will meet your specifi c analysis needs.All confi gurations include gas manifold, accessories, spares, reservoir(s), power cord and operation manual.120 V240 V120 V240 V120 V240 V120 V240 V11CONNECTION TUBING KITSCATALOG NO.DESCRIPTION1100-0450 Tubing Kit to connect Vector PCX & Pinnacle PCX to HPLC1100-0460 Dead-head Plumbing Kit for HPLCNOTE: Dead-head circuits reduce the possibility of corrosion and minimize unswept volume, resulting inmore precise gradient control. Dead-head kits include tee, plug, tubes, fittings.PRODUCT CATALOGPICKERING LABORATORIES10/11

INSTRUMENTS & HARDWARECRX400 POST-COLUMN REACTORSCRX400 Post-column Reactor is designed for use withstandard liquid chromatography equipment.Available standard reaction coil volumes are listed below.If these volumes are not satisfactory, please contact us withyour specic requirements.Replacements or substitution of a reaction coil is easilyaccomplished in the laboratory. Reactor Heater and CoilAssemblies are insulated, self-contained units, comprisingthe reactor coil with inlet and outlet tubing and ttings, andheater with electrical connection.To prevent reactor damage we suggest the installation of apressure relief valve assembly (1100-0710)CRX400 SPECIFICATIONS• Operating temperature starting from 5 °C above ambient to 130 ºC• Set temperature is maintained at ± 0.4 °C within control range• 120 or 240-Volt configurations, 50/60 Hz• Dimensions: (cm) 12 W X 14 H X 28.5 L• Shipping weight : 2 kg (4.4 lbs)PULSE-DAMPENER:FC4870B FLOW CONDITIONERThe Pulse-Dampener is intended for use in Liquid Chromatographyapplications where continuous post-columnderivitization is desired. Installed at the outlet of the pump,the model FC4870B Flow Conditioner provides the pulsedampeningnecessary to maintain a smooth baseline. Alsothe following important features are provided:• Continuous filtering of the pumped medium• Working pressure for the reagent pump: check values performmore reliably which results in a more stable flow• Pressure reading (gauge) for the reagent pump• Prime/purge valveThe gauge and pressure relief valve assembly consists of across for connecting a pressure gauge, an anti-siphon valveand a pre-calibrated relief valve. This is used to indicatepost-column pressure and to prevent rupture of the postcolumnreactor tubing in the event of an overpressurecondition. The anti-siphon valve prevents the reservoirgas pressure from pumping reagent while the system isturned off.PULSE-DAMPENER/FLOW CONDITIONER CATALOG INFORMATIONCATALOG NO.DESCRIPTION1100-0093FC4870B Flow Conditioner, with pressure gauge & prime/purgevalve, for OPA (with 1100-0200 restrictor)1100-0094FC4870B Flow Conditioner, with pressure gauge & prime/purgevalve, for TRIONE® (with 1100-0141 restrictor)1100-0710 Post-column Gauge and Pressure Relief Valve AssemblyCRX400 CATALOG INFORMATIONCATALOG NO. APPLICATION Max.Temp.1100-33291100-33301100-33311100-33321100-33411100-33421100-33231100-33241100-33511100-33521100-33431100-33441100-33251100-33261100-33211100-3322CRX400 Post-column Reactor, 0.15 mL, 120 VCRX400 Post-column Reactor, 0.15 mL, 240 VCRX400 Post-column Reactor, 0.5 mL, 120 VCRX400 Post-column Reactor, 0.5 mL, 240 VCRX400 Post-column Reactor, 1.0 mL, 120 VCRX400 Post-column Reactor, 1.0 mL, 240 VCRX400 Post-column Reactor, 1.2 mL and 1.6 mL, 120 VCRX400 Post-column Reactor, 1.2 mL and 1.6 mL, 240 VCRX400 Post-column Reactor, 1.4 mL, 120 VCRX400 Post-column Reactor, 1.4 mL, 240 VCRX400 Post-column Reactor, 2.0 mL, 120 VCRX400 Post-column Reactor, 2.0 mL, 240 VCRX400 Post-column Reactor, 2.8 mL, 120 VCRX400 Post-column Reactor, 2.8 mL, 240 VCRX400 Post-column Reactor, 3.0 mL, 120 VCRX400 Post-column Reactor, 3.0 mL, 240 V130 ºC130 ºC130 ºC130 ºC130 ºC130 ºC75 ºC75 ºC

INSTRUMENTS & HARDWARESAMPLE CLEAN-UPFLOW RESTRICTORSPickering restrictors are tubes packed with sized diamondparticles designed to provide a desired back-pressure, relativeto a given ow rate and viscosity. The restrictor tubeand ttings are type 316 stainless steel or PEEK. The diamondpacking is chemically and chromatographically inertto all mobile phases.FEATURES• Pressure-calibrated to flow rate and viscosity• Diamond-packed tubes with nuts and ferrules• Improve pump efficiency• Essential pulse-dampening component• Troubleshooting aid for HPLC systemsCATALOG NO.FLOW RESTRICTORS CATALOG INFORMATIONDESCRIPTION1100-0141 Flow restrictor, TRIONE® Ninhydrin reagent, 65 psi, 6 cm1100-0200 Flow restrictor, OPA, Hypochlorite, or Hydrolysis reagent, 300 psi, 10 cm1100-0262 Flow restrictor, 1150 psi, 20 cm1100-0263 Flow restrictor, 550 psi, 17 cm1100-0205 Flow restrictor, 200 psi, 6 cm1100-0142 Flow restrictor, 100 psi, 10 cm1100-0263 Flow restrictor, non-metallic (PEEK), 550 psi, 10 cmNOTE: Listed pressures are for a fl ow rate of 0.3 mL/min at ambient temperaturewith 100 % water and pulse-dampened flow.GLYPHOSATE AND AMPA SAMPLECLEAN-UP CARTRIDGESPickering SPE sample clean-up cartridges are used to treatsamples prior to injection. The strong cation-exchange resinis composed of sulfonic acid functional groups attachedto a styrene divinylbenzene copolymer lattice. The highselectivity and ruggedness provides a simple and universalmethod for removing matrix interference from the sample.The SPE sample clean-up cartridges are ideal for the cleanupof vegetables, fruits and crop samples in the analysis ofGlyphosate and AMPA.RECOVERIES FOR GLYPHOSATE AND AMPAMATRIX GLYPHOSATE AMPAAlfalfa 129 % 116 %Strawberry 84 % 82 %Broccoli 97 % 95 %Sample PreparationExtraction:Take 25 g of homogenous sample and add enough water(after estimation of moisture content) to make the totalvolume of water 125 mL. Blend and Centrifuge.Matrix Speci c Modi cation:• High water content – reduce sample amount to 12.5 g• High protein content – add 100 µL HCl to 20 mL ofextract, shake and centrifuge.• High Fat content – perform the methylene chloridepartition twice.Clean-upMethylene Chloride Partition:• To 20 mL of aqueous extract add 15 mL methylenechloride. Shake for 2-3 min and centrifuge.• To 4.5 mL of aqueous layer add 0.5 mL acidic modiersolution. Shake and centrifuge.SPE Clean-up:• Prepare SPE cartridge (refer to product abstract PA210)• Transfer 1 mL portions of SPE mobile phase (refer tomethod abstract MA206 for formulation) and discardthe efuent.• Elute analytes with 12 mL SPE mobile phaseConcentration:• Evaporate to dryness using rotary evaporator or a vacuumvortex-type evaporator or lyophilize• Re-dissolve in 2 mL of the SPE mobile phase13CATALOG NO. DESCRIPTION QTY1705-0001 SPE sample clean-up cartridges 50/pkgPRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSPickering Laboratories columns and guards are intended for specic applications that require post-column derivatization.This technology guarantees detection of certain classes of compounds at very low concentrations—amino acids, carbamatepesticides and polyamines, for example.Each column is packed and tested to separate the target compounds according to Pickering’s chromatographic qualitycontrol standards and published analytical method. The following acceptance criteria apply to all of Pickering’s columns:• With guard column installed, produce a specied chromatogramof the compounds in a standard test mixture.• Separate the compounds in the established order, with specied resolution of critical pairs.• Operate within the specied range of back pressure.• Be free of contaminating material which can cause baseline artifacts.Only after all criteria have been met can the column’s serial number and label be applied.Quite simply, the column is guaranteed to produce a chromatogram for its intended application if it is operated accordingto the conditions and methods prescribed by Pickering Laboratories.About Guard ColumnsWhile it is true that any of our columns may be run without a guard, thepractical consequence is a shorter column lifetime. Guard columns provideprotection against contamination without affecting column efciency. It isfar less expensive to replace or repack a guard than an analytical column.15PRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSCATION-EXCHANGE COLUMNS & GUARDS CATALOG INFORMATIONCATALOG NO.DESCRIPTIONLITHIUM AMINO ACID ANALYSIS COLUMNS0354100T0354675THigh-efficiency Lithium Cation-exchange Column 4.0 x 100 mm &1700-0070 amino acid test mixture70 min High-efficiency Lithium Ion-exchange Column, 4.6 x 75 mm0393250 Standard Lithium Cation-exchange Column 3.0 x 250 mm &1700-0070 amino acid test mixture0354050 High-efficiency Cation-exchange Column for PKU and MSUD screens 4.0 x 50 mm0352020 Lithium Guard Column, 2.0 x 20 mm (for use with 0354100T)0392020 Lithium Guard Column, 2.0 x 20 mm (for use with 0393250 column)11541501154150T1154110TSODIUM AMINO ACID ANALYSIS COLUMNSHigh-efficiency Sodium Cation-exchange for protein collagen hydrolysates Column 4.0 × 150 mm & 1700-0070amino acid test mixtureHigh-efficiency Sodium Cation-exchange for protein, collagen and oxidized feed hydrolysates Column 4.0 × 150 mm &1700-0070 amino acid test mixture30-minute High-efficiency Sodium Cation-exchange Column 4.6 × 110 mm & 1700-0070 amino acid test mixture1193250 Standard Sodium Cation-exchange Column 3.0 × 250 mm & 1700-0070 amino acid test mixture1193020 Sodium Guard Column, 3.0 × 20 mm (for use with 1154150 and 1154150T Columns)1192020 Sodium Guard Column, 2.0 × 20 mm (for use with 1193250 Column)GLYPHOSATE COLUMNS1954150 Cation-exchange Column for Glyphosate analysis, 4 × 150 mm & 1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL1953020 Cation-exchange Guard Column, 3.0 × 20 mm1952150 Micro Cation-exchange Column, K+ form, 2.1 × 150 mm & 1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL1954250 Column, Glyphosate 4 x 250 mm & 1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mLALKION COLUMN9410917 ALKION Cation-exchange column, K form, 4.0 × 150 mm9493020 ALKION Guard column, K form, 3.0 × 20 mmGARD COLUMN PROTECTION SYSTEM (FOR USE WITH ANY CATION-EXCHANGE COLUMN)1700-3102 Cation-exchange GARD Assembly: Includes holder and 2 replaceable GARDS1700-3101 Replacement Cation-exchange GARDs (2/PK)1700-3100 GARD Holder

CHROMATOGRAMS, COLUMNS & GUARDSREVERSED-PHASE COLUMNS & GUARDS CATALOG INFORMATIONCATALOG NO.DESCRIPTIONCARBAMATE PESTICIDE COLUMNS0840250 Carbamate Column, C 8, expanded resolution, 4.0 × 250 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL1846250 Carbamate Column, C 18, high resolution/capacity, 4.6 × 250 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL1846150 Carbamate Column, C 18, rapid analysis, 4.6 × 150 mm & 1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mLPOLYETHER ANTIBIOTICS ANALYSIS2381750 Polyether reversed-phase column, C 18, 4.6 × 250 mmPARALYTIC SHELLFISH TOXINS ANALYSIS0846150 Reversed-phase column for analysis of paralytic shellfish toxins, C 8, 4.6 × 150 mmMYCOTOXINS ANALYSIS1612124 MYCOTOX Reversed-phase column, C 18, 4.6 × 250 mmGUARDS FOR STANDARD REVERSED-PHASE COLUMNS18ECG00118ECG002Guard Cartridge Holder with 3 cartridgesGuard Cartridges, 2/pkGUARDS FOR MICRO REVERSED-PHASE COLUMNS1700-0204 Guard Cartridge Holder with 3 guard cartridges, for use with Micro columns1700-0205 Guard Cartridges, 2/pk, for use with Micro columnsSPECIALTY COLUMNSCATALOG NO.DESCRIPTION0785150 Anion-exchange Column for Bromate and Nitrite/Nitrate Analysis (4.6 × 150 mm), 5 µm171446250 Amino Column for Voglibose Analysis (4.6 × 250 mm), 5 µmPRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSCATION-EXCHANGE GARD COLUMN PROTECTION SYSTEMThe new GARD uses a proprietary material to prevent matrix compounds from passing through (and thereby protecting theanalytical column), but allows the analytes of interest to pass unimpeded through the GARD and onto the analytical column.The new GARD signicantly prolongs column life without band spreading or added pressure. We demonstrated, bymeans of a performance comparison for Amino Acid Analysis, that the use of a GARD will effectively protect the analyticalcolumn, will be more cost-effective for the laboratory, is easy to change, is universal to cation-exchange applications,and most importantly has zero band spreading.Wth the new GARD Column protection system from Pickering Laboratories the same cation-exchange GARD can beused for nearly all cation-exchange applications.LITHIUM AMINO ACID ANALYSIS COLUMNSPickering Laboratories specialize in manufacturing of cation-exchange columns for amino acid analysis. No other techniques,including reversed-phase chromatography, have been shown to match post-column ion-exchange methods in quantitationand reproducibility. Advantages of this method, such as absence of matrix interferences, are especially important inanalysis of native samples.Lithium columns and buffers systems have high selectivity and are perfect for physiological uids and food analysis.Post-column Conditions For Amino Acids Analysis:Reagent: Trione®Reactor: 130 °C, 0.5 mLReagent Flow Rate: 0.3 mL/minDetection:UV-Vis Detector: =570 nm for primary amino acids=440 nm for secondary amino acidsorReagent: 300 mg of OPA, 2 g Thiouor, 3 mL of 30 %Brij® 35 solution in 950 mL of OD104Reactor: 45 °C, 0.15 mLReagent Flow Rate: 0.3 mL/minDetection:Fluorometer: ex330 nm, em465 nmThe recommended gradient conditions are subject tochange without notice. This may happen because of lotchanges in the columns, or improvements in the overallmethod.The recommended gradient for the column will alwaysbe included in the column package, and it supersedes theinformation in this catalog.Pickering Laboratories recommends six different gradientconditions depending on the column and type of sample.Use the program recommended on the column data sheetfor the initial testing.The column oven temperature programming gives additionalexibility when optimizing methods. Using temperaturegradient allows to improve separation, shorten analysis timeand ne-tune the method for detecting compoundsof interest.

CHROMATOGRAMS, COLUMNS & GUARDS70-MIN HIGH-EFFICIENCY LITHIUM CATION-EXCHANGE COLUMN (4.6 X 75 mm)CATALOG NUMBER 0354675TUSE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEMUSE FOR PHYSIOLOGICAL SAMPLESTEMPERATURE GRADIENTHPLC PROGRAMTIME 1700-1125 % Li365 % Li375 % RG003 %0 100 0 0 010 100 0 0 019 40 60 0 032 0 100 0 043 0 100 0 043.1 0 0 100 057 0 0 100 057.1 0 0 70 3072 0 0 70 3072.1 100 0 0 0HPLC FLOW RATE: 0.55 mL/min, INITIAL TEMP.: 34 ºCCOLUMN OVEN PROGRAMTIME TEMP ºC0 346 3417 6525 7070 7071 34REAGENT FLOW RATE: 0.5 mL/min124457 8 11109202315171618 212419252230453635373938401926 2728 29313323443461214:313341 420 10 20 30 40 50 60 70 min1 Phosphoserine2 Taurine3 Phosphoethanolamine4 Urea5 Aspartic acid6 Hydroxyproline7 Threonine8 Serine9 Asparagine10 Glutamic acid11 Glutamine12 Sarcosine13 -Aminoadipic acid14 Proline15 Glycine16 Alanine17 Citrulline18 -Amino-n-butyric acid19 Valine20 Cystine21 Methionine22 Allo-Isoleucine23 Cystathionine24 Isoleucine25 Leucine26 Tyrosine27 Phenylalanine28 -Alanine29 -Amino-i-butyric acid30 Homocystine31 -Aminobutyric acid32 Tryptophan33 Ethanolamine34 Ammonia35 Hydroxylysines36 Ornitine37 Lysine38 1-Methylhistidine39 Histidine40 3-Methylhistidine41 Anserine42 Carnosine43 Arginine44 Glucosaminic Acid*45 2-Aminoethyl-cysteine (AEC)**Internal StandardNOTE: This method utilizes column temperature gradient. Use Pinnacle PCXcolumn oven or HPLC column oven with temperature gradient capabilities.PRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSHIGH-EFFICIENCY LITHIUM CATION-EXCHANGE COLUMN (4.0 X 100 mm)CATALOG NUMBER 0354100TUSE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR LITHIUM GUARD COLUMN 035202 (2.0 X 20 mm)USE FOR PHYSIOLOGICAL SAMPLES AND COMPLEX MATRICESTEMPERATURE ISOCRATIC1 244351715 16 2018227 8 9 10 11 1213461419212324 2526 27302928313233 34 353637383940414243450 25 5075 1001 Phosphoserine2 Taurine3 Phosphoethanolamine4 Urea5 Aspartic acid6 Hydroxyproline7 Threonine8 Serine9 Asparagine10 Glutamic acid11 Glutamine12 Sarcosine13 Aminoadipic acid14 Proline15 Glycine16 Alanine17 Citrulline18 Amino-n-butyric acid19 Valine20 Cystine21 Methionine22 Cystathionine23 Isoleucine24 Leucine25 Norleucine26 Tyrosine27 Phenylalanine28 Alanine29 Amino-i-butyric acid30 Homocystine31 Aminobutyric acid32 Tryptophan33 Ethanolamine34 Hydroxylysines35 Ammonia36 Creatinine37 Ornithine38 Lysine39 Histidine40 3-Methyl-histidine41 1-Methyl-histidine42 Carnosine43 Anserine44 Amino guanidinopropionic acid45 ArginineCONDITIONSSTEP TIME (Min) INTERVAL Li275 % Li750 % RG003 % COMMENT0 0 0 100 0 0 Inject1 8 8 100 0 0 Isocratic2 46 38 65 35 0 Linear Gradient3 86 40 0 100 0 Linear Gradient4 90 4 0 100 0 Isocratic5 115 25 0 94 6 Linear Gradient6 122 2 0 94 6 Isocratic7 122.1 0.1 100 0 0 Step Change8 140 17.9 100 0 0 Re-equilibrationFLOW RATE: 0.35 mL/min, COLUMN TEMP: 37 ºC,INJECTION VOLUME: 10 µL of 0.25 µmole/mL Std.

CHROMATOGRAMS, COLUMNS & GUARDSHIGH-EFFICIENCY LITHIUM CATION-EXCHANGE COLUMN (4.0 X 100 mm)CATALOG NUMBER 0354100TUSE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR LITHIUM GUARD COLUMN 0352020 (2.0 X 20 mm)USE FOR PHYSIOLOGICAL FLUIDSTEMPERATURE GRADIENTCONDITIONSSTEP TIME (Min) 1700-1125 % Li365 % Li375 % RG003 % COMMENT0 0 100 0 0 0 Inject1 15 100 0 0 0 Isocratic2 27 40 60 0 0 Linear Gradient3 45 0 100 0 0 Linear Gradient4 60 0 100 0 0 Isocratic5 60.1 0 0 100 0 Step Gradient6 78 0 0 100 0 Isocratic7 78.1 0 0 70 30 Step Gradient8 95 0 0 70 30 Isocratic9 95.1 100 0 0 0 Step Gradient10 115 100 0 0 0 EquilibrationHPLC FLOW RATE: 0.4 mL/min, INITIAL TEMP.: 34 ºCCOLUMN OVEN PROGRAMTIME (Min) TEMPERATURE ºC0 3413 3430 6567 6680 7097 7098 341215162017182221587192135363 431023 242526293437 38394691311121427 283032313340 41 420 20 40 6080 min1 Phosphoserine2 Taurine3 Phosphoethanolamine4 Urea5 Aspartic acid6 Hydroxyproline7 Threonine8 Serine9 Asparagine10 Glutamic acid11 Glutamine12 Sarcosine13 -Aminoadipic acid14 Proline15 Glycine16 Alanine17 Citrulline18 -Amino-n-butyric acid19 Valine20 Cystine21 Methionine22 Cystathionine23 Isoleucine24 Leucine25 Tyrosine26 Phenylalanine27 -Alanine28 -Amino-i-butyric acid29 Homocystine30 -Aminobutyric acid31 Tryptophan32 Ethanolamine33 Ammonia34 Hydroxylysines35 Ornitine36 Lysine37 1-Methylhistidine38 Histidine39 3-Methylhistidine40 Anserine41 Carnosine42 Arginine43 Glucosaminic Acid**Internal StandardNOTE: This method utilizes column temperature gradient. Use Pinnacle PCXcolumn oven or HPLC column oven with temperature gradient capabilities.PRODUCT CATALOGPICKERING LABORATORIES10/11

ValineNorleucineCHROMATOGRAMS, COLUMNS & GUARDSHIGH-EFFICIENCY LITHIUM CATION-EXCHANGE COLUMN (4.0 X 100 mm)CATALOG NUMBER 0354100TUSE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR LITHIUM GUARD COLUMN 035202 (2.0 X 20 mm)USE FOR PKU/MSUD SCREENING OF PHYSIOLOGICAL FLUIDSTEMPERATURE ISOCRATICCONDITIONSSTEP TIME (Min) INTERVAL Li275 % Li750 % RG003 % COMMENT0 0 0 86 14 0 Inject1 25 11 73 27 0 Linear Gradient3 25.1 0.1 0 0 100 Step Gradient4 30 16.9 0 0 100 Isocratic7 30.1 0.1 86 14 0 Step Gradient8 42 14.9 86 14 0 Re-equilibrationHPLC FLOW RATE: 0.35 mL/min, COLUMN TEMP.: 38 ºCa-Amino-n-Butyric AcidCystathionineMethionineIsoleucineLeucineTyrosinePhenylalanine5 10 15 2025min

CHROMATOGRAMS, COLUMNS & GUARDSSTANDARD LITHIUM CATION-EXCHANGE COLUMN 0393250 (3.0 X 250 mm)CATALOG NUMBER 0393250USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR LITHIUM GUARD COLUMN 0392020 (2.0 X 20 mm)USE FOR PHYSIOLOGICAL FLUIDS, COMPLEX MATRICESTEMPERATURE ISOCRATIC123442022403958710912 131114171816 19153023 24252126 2728 29313234353337 38414243452346360 20 40 60 80 100 120 140 160 180min1 Phosphoserine2 Taurine3 Phosphoethanolamine4 Urea5 Aspartic acid6 Hydroxyproline7 Threonine8 Serine9 Asparagine10 Glutamic acid11 Glutamine12 Sarcosine13 Aminoadipic acid14 Proline15 Glycine16 Alanine17 Citrulline18 Amino-n-butyric acid19 Valine20 Cystine21 Methionine22 Cystathionine23 Isoleucine24 Leucine25 Norleucine26 Tyrosine27 Phenylalanine28 Alanine29 Amino-i-butyric acid30 Homocystine31 Aminobutyric acid32 Tryptophan33 Ethanolamine34 Hydroxylysines35 Ammonia36 Creatinine37 Ornithine38 Lysine39 Histidine40 3-Methyl-histidine41 1-Methyl-histidine42 Carnosine43 Anserine44 Amino guanidino propionic acid45 ArginineCONDITIONSTIME (Min) Li275 % Li750 % RG003 %0 100 0 017 100 0 065 65 35 0128 0 100 0145 0 100 0185 0 94 6185.1 100 0 0210 100 0 0FLOW RATE: 0.3 mL/min, COLUMN TEMP.: 40 ºC,INJECTION VOLUME: 10 L OF 0.25 mole/mL STD.PRODUCT CATALOGPICKERING LABORATORIES10/11

MetTyrPheMetTyrLeu + IleLeu + IlePheCHROMATOGRAMS, COLUMNS & GUARDSHIGH-EFFICIENCY CATION-EXCHANGE COLUMN (4.0 X 50 mm)CATALOG NUMBER 0354050USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR LITHIUM GUARD COLUMN 0352020 (2.0 X 20 mm)USE FOR PKU/MSUD SCREENING OF PHYSIOLOGICAL FLUIDSTEMPERATURE ISOCRATIC01 23 4 5 6 7 8minNormal Neonate serum01 2 3 4 5 6 7 8minNeonate serum with elevated PhenylalanineCONDITIONSTIME (Min) Li357 % RG003 %0 100 08 100 08.1 0 10010 0 10010.1 100 016 100 0FLOW RATE: 0.4 mL/min, COLUMN TEMP.: 65 ºC,INJECTION VOLUME: 10 LLITHIUM COLUMNS CATALOG INFORMATIONCATALOG NO.DESCRIPTION0354050 High-efficiency Cation-exchange Column for PKU and MSUD screens 4.0 x 50 mm0354675T0354100T70-min High-efficiency Lithium Ion-exchange Column, 4.6 x 75 mmHigh-efficiency Lithium Cation-exchange Column 4.0 x 100 mm &1700-0070 amino acid test mixture0393250 Standard Lithium Cation-exchange Column 3.0 x 250 mm &1700-0070 amino acid test mixture0352020 Lithium Guard Column, 2.0 x 20 mm (for use with 0353100T or 0354050 column)0392020 Lithium Guard Column, 2.0 x 20 mm (for use with 0393250 column)GARD COLUMN PROTECTION SYSTEM (FOR USE WITH ANY CATION-EXCHANGE COLUMN)CATALOG NO.DESCRIPTION1700-3102 Cation-exchange GARD Assembly: Includes holder and 2 replaceable GARDS1700-3101 Replacement Cation-exchange GARDs (2/PK)1700-3100 GARD Holder

CHROMATOGRAMS, COLUMNS & GUARDSSODIUM AMINO ACID ANALYSIS COLUMNSIon-exchange chromatography followed by post-columnderivatization has been a method of choice for amino acidanalysis for many years. Pickering ion-exchange columnsallow you to obtain consistent results with sensitivity, stability,selectivity and speed. Sodium columns and buffers systems aredesigned for amino acids analysis of hydrolyzed samples.Post-column Conditions For Amino Acids Analysis:Reagent: Trione®Reactor: 130 °C, 0.5 mLReagent Flow Rate: 0.3 mL/minDetection:UV-Vis Detector: =570 nm for primary amino acids=440 nm for secondary amino acidsorReagent: 300 mg of OPA, 2 g Thiouor, 3 mL of 30 %Brij® 35 solution in 950 mL of OD104Reactor: 45 °C, 0.15 mLReagent Flow Rate: 0.3 mL/minDetection:Fluorometer: ex330 nm, em465 nmThe recommended gradient conditions are subject tochange without notice. This may happen because of lotchanges in the columns, or improvements in the overallmethod.The recommended gradient for the column will alwaysbe included in the column package, and it supersedes theinformation in this catalog.Pickering Laboratories recommends six different gradientconditions depending on the column and type of sample.Use the program recommended on the column data sheetfor the initial testing.The column oven temperature programming gives additionalexibility when optimizing methods. Using temperaturegradient allows to improve separation, shorten analysistime and ne-tune the method for detecting compoundsof interest.25PRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDS30-MIN HIGH-EFFICIENCY SODIUM CATION-EXCHANGE COLUMN (4.6 X 110 mm)CATALOG NUMBER 1154110TUSE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEMUSE FOR PROTEIN AND COLLAGEN HYDROLYSATETEMPERATURE GRADIENTHPLC PROGRAMTIME Na315 % Na740 % RG011 %0 100 0 07.3 100 0 015 53 47 015.1 0 100 028 0 100 028.1 0 0 10030 0 0 10030.1 100 0 039 100 0 0HPLC FLOW RATE: 0.6 mL/min, INITIAL TEMP.: 46 ºCCOLUMN OVEN PROGRAMTIME TEMP ºC0 463 4610 5522 7027 7028 46REAGENT FLOW RATE: 0.5 mL/min8171 2 36 791011 1312141516ProteinHydrolysate420181950 10 20 301 Aspartic Acid2 Threonine3 Serine4 Glutamic Acid5 Proline6 Glycine7 Alanine8 Cystine9 Valine10 Methionine11 Isoleucine12 Leucine13 Norleucine14 Tyrosine15 Phenylalanine16 Lysine17 Histidine18 Ammonia19 Tryptophan20 ArginineminNOTE: This method utilizes column temperature gradient. Use Pinnacle PCXcolumn oven or HPLC column oven with temperature gradient capabilities.

CHROMATOGRAMS, COLUMNS & GUARDSHIGH-EFFICIENCY CATION-EXCHANGE COLUMN (4.0 X 150 mm)CATALOG NUMBER 1154150TUSE WITH GARD COLUMN PROTECTION SYSTEM OR SODIUM GUARD COLUMN 1193020 (3.0 X 20 mm)USE FOR OXIDIZED HYDROLYSATE, PROTEIN HYDROLYSATE, COLLAGEN HYDROLYSATETEMPERATURE ISOCRATIC23243 4 5 67891113 14160 10 20 30 40 50181920OxidizedHydrolysate22minCONDITIONS FOR OXIDIZED SAMPLES 1154150TTIME (Min) Na270 % Na740 % RG011 %0 100 0 014 100 0 032 20 80 032.1 0 100 056 0 100 056.1 0 0 10058 0 0 10058.1 100 0 070 100 0 0FLOW RATE: 0.4 mL/min, COLUMN TEMP.: 50 ºC,INJECTION VOLUME: 10 L OF 0.25 mole/mL STD.3 Aspartic Acid4 Threonine5 Serine6 Glutamic Acid7 Proline8 Glycine9 Alanine11 Valine13 Isoleucine14 Leucine16 Phenylalanine18 Lysine19 Ammonia20 Histidine22 Arginine23 Cysteic Acid24 Methionine Sulfone2710111291820131415 16178119 12 1813 1415 1620ProteinHydrolysate13 456810CollagenHydrolysate3 4 5 619192272272120 10 20 30 40 50min0 10 20 30 40 50min1 Methionine-D,L,-Sulfoxide2 trans-4-Hydroxy-L-Proline3 Aspartic Acid4 Threonine5 Serine6 Glutamic Acid7 Proline8 Glycine9 Alanine10 Cystine11 Valine12 Methionine13 Isoleucine14 Leucine15 Tyrosine16 Phenylalanine17 D,L & allo-Hydroxylysine18 Lysine19 Ammonia20 Histidine21 Tryptophan22 ArginineCONDITIONS FOR PROTEIN &COLLAGEN HYDROLYSATE SAMPLESTIME (Min) Na315 % Na740 % RG011 %0 100 0 010 100 0 030 0 100 053 0 100 053.1 0 0 10055 0 0 10055.1 100 0 067 100 0 0FLOW RATE: 0.4 mL/min, COLUMN TEMP.: 48 ºC,INJECTION VOLUME: 10 L OF 0.25 mole/mL STD.PRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSHIGH-EFFICIENCY SODIUM CATION-EXCHANGE COLUMN (4.0 X 150 mm)CATALOG NUMBER 1154150USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR SODIUM GUARD COLUMN 1193020 (3.0 X 20 mm)USE FOR PROTEIN HYDROLYSATE, COLLAGEN HYDROLYSATETEMPERATURE ISOCRATIC1053 491112131418208Protein Hydrolysate615 1619222175349811121314182010Collagen Hydrolysate161516 1719222705 10 15 20 25 30 35 40 45 50 55 60min1 Methionine-D,L,-Sulfoxide2 trans-4-Hydroxy-L-Proline3 Aspartic Acid4 Threonine5 Serine6 Glutamic Acid7 Proline8 Glycine9 Alanine10 Cystine11 Valine12 Methionine13 Isoleucine14 Leucine15 Tyrosine16 Phenylalanine17 D,L & allo-Hydroxylysine18 Lysine19 Ammonia20 Histidine21 Tryptophan22 ArginineCONDITIONSTIME (Min) 1700-0112 % Na740 % RG011 %0 100 0 012 100 0 034 0 100 053 0 100 053.1 0 0 10055 0 0 10055.1 100 0 067 100 0 0FLOW RATE: 0.4 mL/min, COLUMN TEMP.: 48 ºC,INJECTION VOLUME: 10 L OF 0.25 mole/mL STD.

CHROMATOGRAMS, COLUMNS & GUARDSSTANDARD SODIUM CATION-EXCHANGE COLUMN (3.0 X 250 mm)CATALOG NUMBER 1193250USE WITH CATION-EXCHANGE GARD COLUMN PROTECTION SYSTEM OR SODIUM GUARD COLUMN 1192020 (2.0 X 20 mm)USE FOR PROTEIN HYDROLYSATETEMPERATURE ISOCRATIC34511131418201019689Protein Hydrolysate121516227212905 10 15 20 25 30 35 40 45 50 55 60min3 Aspartic acid4 Threonine5 Serine6 Glutamic acid7 Proline8 Glycine9 Alanine10 Cystine11 Valine12 Methionine13 Isoleucine14 Leucine15 Tyrosine16 Phenylalanine17 Hydroxylysines18 Lysine19 Ammonia20 Histidine21 Tryptophan22 ArginineSODIUM COLUMNS CATALOG INFORMATIONCATALOG NO.DESCRIPTION1154110T30-minute High-efficiency Sodium Cation-exchange Column 4.6 × 110 mm & 1700-0070 aminoacid test mixture1154150THigh-efficiency Sodium Cation-exchange for protein, collagen and oxidized feed hydrolysatesColumn 4.0 × 150 mm & 1700-0070 amino acid test mixture1154150High-efficiency Sodium Cation-exchange for protein and collegen hydrosates Column4.0 × 150 mm & 1700-0070 amino acid test mixture1193250 Standard Sodium Cation-exchange Column 3.0 × 250 mm & 1700-0070 amino acid test mixture1193020 Sodium Guard Column, 3.0 × 20 mm (for use with 1154150 Column)1192020 Sodium Guard Column, 2.0 × 20 mm (for use with 1193250 Column)CONDITIONS FOR PROTEIN HYDROLYSATE SAMPLESTIME (Min) Na328 % Na740 % RG011 %0 100 0 010 100 0 032 0 100 056 0 100 056.1 0 0 10058 0 0 10058.1 100 0 070 100 0 0FLOW RATE: 0.3 mL/min, COLUMN TEMP.: 48 ºC,INJECTION VOLUME: 10 L OF 0.25 mole/mL STD.GARD COLUMN PROTECTION SYSTEM(FOR USE WITH ANY CATION-EXCHANGE COLUMN)CATALOG NO.DESCRIPTION1700-3102 Cation-exchange GARD Assembly: Includes holder and 2 replaceable GARDS1700-3101 Replacement Cation-exchange GARDs (2/PK)1700-3100 GARD HolderPRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSCARBAMATE PESTICIDE ANALYSIS COLUMNSPickering Laboratories carbamate columns are guaranteed to produce the separation of carbamate residues, specied byEPA and AOAC methods. For C 18columns, two water/methanol gradient conditions are available for analysis of methanolicand water samples. Expanded resolution C 8column is capable of separating as many as 23 carbamates with eitherwater/methanol or water/acetonitrile gradients. The difference in selectivity between Methanol and Acetonitrile protocolscould be used for conrming peak identication.Post-column conditions for pesticide analysis:Reagent 1: Hydrolysis reagent CB130 or CB130.2Reagent 2: 100 mg of OPA, 2 g Thiouorin 950 mL of CB910Reactor 1: 100 °C, 0.5 mLReactor 2: ambient. 0.1 mLReagents ow rate: 0.3 mL/minDetection:Fluorometer: ex330 nm, em465 nmThe recommended gradient conditions are subject tochange without notice. This may happen because of lotchanges in the columns, or improvements in the overallmethod.The recommended gradient for the column will alwaysbe included in the column package, and it supersedes theinformation in this catalog.CARBAMATE COLUMN 1846250 (4.6 X 250 mm), C 18 , 5 µm1423654 ng of Carbamates in 150 µL91178 12100 5 10 15 20 25 30 35 40 45minCONDITIONS FORAQUEOUS SAMPLESTIME (Min) WATER % MeOH %0 100 01 100 01.1 82 1836 30 7039 30 7039.1 0 10041 0 10041.1 100 055 100 0FLOW RATE: 1 mL/min,COLUMN TEMP.: 42 ºC,INJECTION VOLUME: Up to 400 L05 10 15 20 25 30 35 40 45 501 Aldicarb sulfoxide2 Aldicarb sulfone3 Oxamyl4 Methomyl123455 3-Hydroxycarbofuran6 Aldicarb7 Propoxur8 Carbofuran625 ng of Carbamates in 10 µL7891011 12min9 Carbaryl10 1-Naphthol11 Methiocarb12 BDMC (internal standard)CONDITIONS FORMETHANOLIC SAMPLESTIME (Min) WATER % MeOH %0 85 151 85 1544 25 7544.1 0 10049 0 10049.1 85 1557 85 15FLOW RATE: 1 mL/min,COLUMN TEMP.: 42 ºC,INJECTION VOLUME: 10 L

CHROMATOGRAMS, COLUMNS & GUARDSCARBAMATE COLUMN 1846150 (4.6 X 150 mm), C 18 , 5 µmCONDITIONS FORAQUEOUS SAMPLESTIME (Min) WATER % MeOH %0 100 01 100 01.1 88 1226 30 7026.1 0 10028 0 10028.1 100 038 100 0FLOW RATE: 1 mL/min,COLUMN TEMP.: 42 ºC,INJECTION VOLUME: Up to 250 L0412354 ng of Carbamates in 150 µL65 10 15 20 25 30min78910 1112CONDITIONS FORMETHANOLIC SAMPLESTIME (Min) WATER % MeOH %0 85 150.5 85 1528.5 30 7028.6 0 10033.5 0 10033.6 85 1541 85 15FLOW RATE: 1 mL/min,COLUMN TEMP.: 42 ºC,INJECTION VOLUME: 10 L01 2 34567891011125 10 15 20 25 30min1 Aldicarb sulfoxide2 Aldicarb sulfone3 Oxamyl4 Methomyl5 3-Hydroxycarbofuran6 Aldicarb7 Propoxur25 ng of Carbamates in 10 µL8 Carbofuran9 Carbaryl10 1-Naphthol11 Methiocarb12 BDMC (internal standard)31PRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSCARBAMATE COLUMN 0840250 (4.0 X 250 mm), C 8 , 5 µmCONDITIONS FORWATER/METHANOL GRADIENTTIME (Min) WATER % MeOH %0 85 152 85 1542 30 7046 30 7046.1 0 10051 0 10051.1 85 1559 85 15FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 50 ºC,INJECTION VOLUME: 10 L formethanolic and up to 400 L foraqueous samples041623525 ng of Carbamates in 10 µL97 10811125 10 15 20 25 30 35 40 45 50minCONDITIONS FORWATER/ACETONITRILEGRADIENTTIME (Min) WATER % ACN %0 90 102 90 1046 49 5146.1 30 7049 30 7049.1 90 1059 90 10FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 50 ºC,INJECTION VOLUME: 10 L formethanolic and up to 400 L foraqueous samples040 pg of Carbamates in 400 µL9134611571281025 10 15 20 25 30 35 40 45 50min1 Aldicarb sulfoxide2 Aldicarb sulfone3 Oxamyl4 Methomyl5 3-Hydroxycarbofuran6 Aldicarb7 Propoxur8 Carbofuran9 Carbaryl10 1-Naphthol11 Methiocarb12 BDMC (internal standard)

CHROMATOGRAMS, COLUMNS & GUARDSSEPARATION OF 23 CARBAMATES USING 0840250 COLUMN (4.0 X 250 mm)CONDITIONS FORWATER/METHANOL GRADIENTTIME (Min) WATER % MeOH %0 85 152 85 1542 30 7046 30 7046.1 0 10051 0 10051.1 85 1559 85 15FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 50 ºC,INJECTION VOLUME: 10 L formethanolic and up to 400 L foraqueous samples1 Aldicarb sulfoxide2 Aldicarb sulfone3 Oxamyl4 Methomyl5 3-Hydroxy6 Aldicarb7 Propoxur8 Carbofuran9 Carbaryl10 Methiocarb11 BDMC internal standard12 Butocarboxim sulfoxide13 Butocarboxim sulfone14 Ethiofencarb sulfoxide15 Ethiofencarb sulfone16 Butocarboxim17 Ethiofencarb18 Thiofanox sulfoxide19 Thiofanox sulfone20 Methiocarb sulfoxide21 Methiocarb sulfone22 3-Ketocarbofuran23 ThiofanoxN 1-Naphthol49162357 N8101142012132141396167151719215111822 810235 10 15 20 25 30 35 40 45 min33CATALOG NO.CARBAMATE COLUMNS CATALOG INFORMATIONDESCRIPTION0840250 Carbamate Column, C 8, expanded resolution, 4.0 × 250 mm &1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL1846250 Carbamate Column, C 18, high resolution/capacity, 4.6 × 250 mm &1700-0063 Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL1846150 Carbamate Column, C 18, rapid analysis, 4.6 × 150 mm & 1700-0063Carbamate Test Mixture, 2.5 µg/mL, 1.5 mL18ECG00118ECG002Guard Cartridge Holder with 3 guard cartridgesGuard Cartridges, 2/pk.PRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSGLYPHOSATE HERBICIDE ANALYSIS COLUMNPickering Laboratories cation-exchange Glyphosate columnis designed and tested for analysis of Glyphosate and itsprimary metabolite Aminomethylphosphonic acid (AMPA).This short, isocratic method provides separation of thepeaks of interest. Reproducible performance is guaranteedrun-to-run and column-to-column.Post-column Conditions For Glyphosate Analysis:Reagent 1: Oxidizing reagent—100 µL of 5 % SodiumHypochlorite in 950 mL of GA116Reagent 2: 100 mg of OPA, 2 g Thiouor in 950 mL of GA104Reactor 1: 36 °C, 0.5 mLReactor 2: ambient. 0.1 mLReagents Flow Rate: 0.3 mL/minDetection:Fluorometer: ex330 nm, em465 nmGLYPHOSATE COLUMN 1954150 (4.0 X 150 mm) K+ CATION-EXCHANGE.TO BE USED WITH CATION-EXCHANGE GARD COLUMN PROTECTIONSYSTEM OR GUARD COLUMN (3.0 X 20 mm), K+ CATION-EXCHANGEGlyphosateAMPA0 10 20 minGlyphosate Test Mixture, 2.5 µg/mLCONDITIONSTIME (Min) K200 % RG019 %0 100 015 100 015.1 0 10017 0 10017.1 100 027 100 0FLOW RATE: 0.4 mL/min,COLUMN TEMP.: 55 ºC,INJECTION VOLUME: 10 LGLYPHOSATE COLUMNS CATALOG INFORMATIONCATALOG NO.DESCRIPTION1954150Cation-exchange Column for Glyphosate analysis, 4 × 150 mm &1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL1953020 Cation-exchange Guard Column, 3.0 × 20 mm1952150Micro Cation-exchange Column, K+ form, 2.1 × 150 mm & 1700-0080 Glyphosate test mixture, 2.5 µg/mL, 1.5 mL1954250Column, Glyphosate 4 x 250 mm & 1700-0080 Glyphosate testmixture, 2.5 µg/mL, 1.5 mLCATALOG NO.1700-3102GARD COLUMN PROTECTION SYSTEM(FOR USE WITH ANY CATION-EXCHANGE COLUMN)DESCRIPTIONCation-exchange GARD Assembly: Includes holder and2 replaceable GARDS1700-3101 Replacement Cation-exchange GARDs (2/PK)1700-3100 GARD Holder

CHROMATOGRAMS, COLUMNS & GUARDSALKION COLUMNThe stationary phase in the ALKION column is a rigid,non-porous polymeric phase that is surface sulfonated. Thetwo modes of separation are ion-exchange and moderatepartitioning. The low capacity of the ion-exchange resinmakes it an ideal phase for the separation of strongly basicand positively charged compounds. The reversed-phasecharacter allows for discrimination between closely relatedspecies.123 45The unique properties of this column allow using it for awide range of applications.ALKION COLUMN ANALYSIS OF BIOGENIC AMINESPost-column Conditions:Reagent 1: 300 mg of OPA, 2 g Thiouor, 3 mL of 30 %Brij® 35 in 950 mL of OD104Reactor: 45 °C, 0.15 mLReagents Flow Rate: 0.3 mL/minDetection:Fluorometer: ex330 nm, em465 nm01 Histamine2 Putrescine3 Cavaverine5 10 15 20minCONDITIONS4 Spermidine5 SpermineTIME (Min) K600 % K563 % K130 %0 100 0 06 100 0 015 0 100 021 0 100 021.1 0 0 10023 0 0 10023.1 100 0 029 100 0 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 45 ºC,INJECTION VOLUME: 10 L35Histamine5 Days Old5 Days OldPutrescineCadaverineFresh0 2 4 6 8 10minAhi Tuna Fillet extracted per FDA MethodHistaminePutrescineCadaverineFresh0 2 4 6 8 10minMahi-Mahi extracted per FDA MethodCONDITIONSTIME (Min) K600 % K130 %0 100 012 100 012.1 0 10015 0 10015.1 100 020 100 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 45 ºC,INJECTION VOLUME: 10 LPRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSALKION COLUMN ANALYSIS OF AMINOGLYCOSIDEANTIBIOTICS IN FEEDSPost-column Conditions:Reagent 1: 300 mg of OPA, 2g Thiouor, 3 mL of 30 %Brij® 35 in 950 mL of OD104Reactor: 45 °C, 0.15 mLReagents Flow Rate: 0.3 mL/minDetection:Fluorometer: ex330 nm, em465 nmApramycinCONDITIONS FOR APRAMYCIN ANALYSISTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 67 33 05 67 33 015 14.7 7.3 7820 14.7 7.3 7820.1 0 22 7821 0 22 7821.1 67 33 028 67 33 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: 10 L05 10 15 20 25 30minGentamicinComplexCONDITIONS FOR GENTAMICIN ANALYSISTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 43 31 2620 9 13 7830 9 13 7830.1 0 22 7831 0 22 7831.1 43 31 2638 43 31 26FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: 10 L05 10 15 20 25 30minNeomycinCONDITIONS FOR NEOMYCIN ANALYSISTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 60 40 015 13.2 8.8 7825 11 11 7825.1 0 22 7826 0 22 7826.1 60 40 032 60 40 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: 10 L05 10 15 20 25 30min

CHROMATOGRAMS, COLUMNS & GUARDSCONDITIONS FOR SEPARATION OF APRAMYCINGENTAMICIN AND NEOMYCINTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 60 40 015 13.2 8.8 7815.1 12 10 7830 12 10 7830.1 0 22 7831 0 22 7831.1 60 40 037 60 40 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: 10 LNeomycin37GentamicinComplexApramycin05 10 15 20 25 30minPRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSALKION COLUMN ANALYSIS OF PARAQUAT ANDDIQUAT IN WATERPost-column Conditions:Reagent 1: 300 mg/L of Sodium Dithionite in0.3 M Sodium HydroxideReactor: 35 °C, 0.15 mLReagents Flow Rate: 0.3 mL/minDetection:UV-Vis Detector: 395 nm for PQ, 378 nm for DQCONDITIONSTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % i-PROPANOL %0 31.5 53.5 0 1510 28 47 10 1515 28 47 10 1515.1 0 85 10 517 0 85 10 517.1 31.5 53.5 0 1523 31.5 53.5 0 15FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 50 ºC,INJECTION VOLUME: up to several mLDQALKION COLUMN ANALYSIS OF STREPTOMYCINPost-column Conditions:Reagent 1: 0.75 N Sodium HydroxideReactor 1: ambient, 0.1 mLReagent 2: 0.6 % NinhydrinReactor 2: 60 °C, 0.5 mLReagents Flow Rate: 0.3 mL/minDetection:Fluorometer: ex395 nm, em500 nmorReagent 1: Oxidizing Reagent: 100 µL of 5 %Sodium Hypochlorite in GA116Reactor 1: 50 °C, 0.5 mLReagent 2: 300 mg of OPA, 2 g Thiouor, 3 mL of 30 %Brij® 35 solution in 950 mL of OD104Reactor 2: ambient, 0.1 mLDetection:Fluorometer: ex330 nm, em465 nmPQPQ=ParaquatDQ=DiquatCONDITIONSTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 60 40 010 60 40 010.1 48 48 415 48 48 415.1 60 40 021 60 40 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: up to several LStreptomycin68 10 12minNOTE: Above chromatogram is approximately 1 ppm in 200 µL.ALKION COLUMN CATALOG INFORMATIONCATALOG NO.DESCRIPTION9410917 ALKION Cation-exchange column, K form, 4.0 × 150 mm9493020 ALKION Guard column, K form, 3.0 × 20 mm0 5 10 15min

CHROMATOGRAMS, COLUMNS & GUARDSAFLATOXINS ANALYSISAatoxins are naturally occurring toxins belonging to the class Mycotoxins. They are produced by fungi and occurin peanuts, peanut meal, cotton-seed meal, wheat, milk and many other foods and feeds.The most important feature of the post-column method described below is that all six Aatoxins are detectableat the same uorescence emission wavelength in a single isocratic HPLC analysis.LC Conditions:Post-column Conditions:Flow Rate: 1.0 mL/min, column temperature 42 °C, Reagent: I 2100 mg/L in waterinjection volume 10 µLReactor: 70 °C, 1.4 mLMobile Phase: Methanol/acetonitrile/water:Reagent Flow Rate: 0.4 mL/min(22:22:56), isocraticDetection:Injection: 10 µL in MethanolFluorometer: 5 ng B 1& Gex365 nm, em430 nm1.5 ng B 2& G 21.25 ng M 1& M 2G1 B2B1M2M1G202 468 10 12 14minMYCOTOX COLUMN CATALOG INFORMATIONCATALOG NO.DESCRIPTION1612124 MYCOTOX Reversed-phase column, C 18, 4.6 × 250 mm18ECG001 Guard Cartridge Holder with 3 cartridges18ECG002 Guard Cartridges, 2/pk.

CHROMATOGRAMS, COLUMNS & GUARDSPOLYETHER ANTIBIOTICS ANALYSIS COLUMNPolyether antibiotics, such as Monensin, Narasin and Salinomycin, found in raw material, premix, liquid supplements andnal feeds, are best quantied using HPLC with post-column derivatization. Because of selectivity of post-column reaction,almost no sample clean-up is needed.LC Conditions:Flow Rate: 0.7 mL/min, column temperature 40 °C,injection volume 10 µLMobile Phase: Methanol/5 % Acetic Acid inWater (90:10), isocraticFeed Extract2Post-column Conditions(non-metallic post-column derivatization system is required):Reagent 1: Concentrated Sulfuric acid / Methanol (4:96 v/v)Reactor 1: Ambient, 0.1 mLReagent 2: 60 g of Vanillin (or p-dimethylaminobenzaldehyde)in 950 mL of MethanolReactor 2: 90 °C, 1.4 mLReagents Flow Rate: 0.3 mL/minNOTE: Using 2-reagent system for this application greatly extends reagents life.One-reagent method and post-column derivatizationsystem are also available.Detection:UV-Vis detector: Vanillin =520 nm, DMAB =450 nm14323Standard10510 15min1 Monensin B 2 Monensin A 3 Narasin APOLYETHER ANTIBIOTICS CATALOG INFORMATIONCATALOG NO.DESCRIPTION2381750 Polyether reversed-phase column, C 18, 4.6 × 250 mm18ECG001 Guard Cartridge Holder with 3 cartridges18ECG002 Guard Cartridges, 2/pk.PRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSVOGLIBOSE ANALYSIS COLUMNMETHODAnalytical ConditionsColumn: Amino column,4.6x250 mm,Catalog number 1446250Temperature: 35 °CFlow Rate: 0.6 mL/minMobile Phase: Sodium phosphate buffer, 20 mMpH 6.5 / Acetonitrile (37 : 63)Injection Volume: 50 µLSample PreparationCrush 5 tablets and mix with 25 mL of mobile phase.Sonicate for 10 min and lter liquid portion through0.45 µm lter. Put in HPLC autosampler vial and inject 50 µL.Post-column ConditionsPost-column System: Pinnacle PCXHeated Reactor Volume: 3.5 mLTemperature: 100 °CCooling Coil: 0.15 mL (at room temperature)Reagent: Taurine (6.25 g), Sodium Periodate (2.56 g)in 1000 mL of waterFlow Rate: 0.6 mL/minDetection: FLD ex: 350 nm, em: 430 nmVogliboseVoglibose0 5 10 15 20 25min0 5 10 15 20 25minChromatogram of Voglibose Standard,50 ppm, 50 µL InjectionChromatogram of Voglibose Tablets (Volix TM , 0.2 mg),50 µL InjectionVOGLIBOSE ANALYSIS CATALOG INFORMATIONCATALOG NO.DESCRIPTION1446250 Amino Column, 4.6 × 250 mm, 5µm

CHROMATOGRAMS, COLUMNS & GUARDSANION-EXCHANGE COLUMNNITRITE & NITRATE ANALYSISAOAC Ofcial method 993.03 1 for the analysis of nitrate involves reduction using spongy Cadmium which is toxic andcarcinogenic. FDA improved on this method by using Vanadium(III) chloride and heat 2 for the post-column reduction ofnitrate to nitrite. Nitrite reacts with this modied Griess reagent to produce a red chromophore with maximal absorbanceat 535nm. Pickering Laboratories Inc. has further improved this method by substituting the corrosive hydrochloric acid withmethane sulfonic acid.METHODAnalytical ConditionsColumn: Anion exchange column4.6 x 150 mm,Catalog number 0785150Temperature: 50 °CSample Injection Volume: 10 µLLC Flow Rate: 1 mL/minMobile Phase: 20 mM sodium chlorate and10 mM dihydrogensodium phosphate0.120.10NitritePost-column ConditionsPost-column System: PinnacleReactor Volume: 0.5 mLReactor Temperature: 100 °CReagent Flow Rate: 0.1 mL/minReagent: (i) 1 % Vanadium(III) chloride in 20 % methanesulfonic acid(ii) 1 % m-Nitro aniline in 20 % methanesulfonic acid(iii) 1 % N-(1-Naphthyl)ethylenediaminedihydrochloride in 20 % methanesulfonic acidMix 50 mL of (i) and (ii), and 1.25 mL of (iii) anddilute to 250 mL using 20 % methanesulfonic acidDetection: UV/VIS, max= 535 nmSample PreparationTo 5 g of baby food in a 50 mL centrifuge tube, add 25 mLof 50-60 °C water (for vegetables) or 15 mM Sodium acetate(for fruits) and shake for 10 min. Add 12.5 mL of acetonitrileand make up the volume to 50 mL using water (for vegetables)and sodium acetate (for fruits). Centrifuge the mixturefor 15 mins at 5000 rpm. Filter the supernatant through a0.45 µ nylon lter and dilute to fall within the linear range.NOTESPost-column reagent solutions are stored in plastic containers.All solutions are fi ltered through 0.45 µ nylon fi lter before use.Nitrate/Nitrite standards should be checked prior to use for oxidation.Sample pH should be checked to determine the choice of extraction solutionsince acidic pH facilitates the conversion of nitrite to nitrate.45AU0.080.060.040.020.00Nitrate2.00 4.00 6.00 8.00 10.00 12.00 14.00min20 ppm Nitrite and Nitrate StandardACKNOWLEDGMENTSJohn A. Casanova, Food and DrugAdministration, 60 8 th Street, Atlanta,GA 30309REFERENCES1. AOAC- Offi cial Methods of Analysisof AOAC International (2000) 17 th Ed.,Section 50.1.11.Use of Griess Reagents ContainingVanadium (III) for the Post-columnDerivatization and SimultaneousDetermination of Nitrite and Nitrite inBaby Food, John A. Casanova, Lois K.Gross, Sarah E. McMullen and FrankSchenck, Food and Drug Administration,60 8 th Street, Atlanta, GA 30309.PRODUCT CATALOGPICKERING LABORATORIES10/11

CHROMATOGRAMS, COLUMNS & GUARDSANALYSIS OF BROMATE IN DRINKING WATERBromate is a disinfection by-product that is formed when Ozone reacts with naturally occurring Bromide in drinkingwater. Bromate is a known animal carcinogen and has also been listed as a group 2B toxin: probable human carcinogen.The USEPA Method 300.1 employs conductivity as the means of detection which works well for most anions. However,the method is non-specic and coeluting interferences cannot be identied. The more recent USEPA Method 317.0utilizes a Bromate specic reagent in a post-column reaction. This allows for a very specic and sensitive assay forBromate in complex matrices.METHODAnalytical ConditionsColumn: Anion exchange column4.6 x 150 mm,Catalog number 0785150Temperature: 42 ºCSample Injection Volume: 250 µLFlow Rate: 1.3 mL/minMobile Phase: 9 mM Na 2CO 3Post-column ConditionsPost-column system: Pinnacle PCXReactor Volume: 0.5 mLReactor Temperature: 60CReagent:o–Dianisidine dihydrochloride (Add 40 mL of 70 % HNO 3to 300mL deionized water in a 500mL volumetric ask. Dissolve2.5g KBr in this solution. Dissolve 250 mg ofo–Dianisidine dihydrochloride in 100 mL of Methanol andadd to the Nitric acid/KBr solution and dilute to volume.)Flow Rate: 0.7 mL/minDetection: UV/VIS detector, max = 450 nmuAU2.010ppb1.58ppb1.05ppb0.50.02.5ppb-0.41.50 1.75 2.00 2.25 2.50 2.75 3.00minNITRITE/NITRATE & BROMATE ANALYSIS CATALOG INFORMATIONCATALOG NO.DESCRIPTION0785150 Anion-exchange column, (4.6 x 150 mm), 5 µm

CHROMATOGRAMS, COLUMNS & GUARDSANALYSIS OF ALENDRONIC ACID IN PHARMACEUTICAL FORMULATIONSAlendronic acid or Alendronate Sodium is a bisphosphonate drug that inhibits osteoclast-mediated bone resorption.Biphosphates work by binding to the bone and preventing calcium from being removed by osteoclasts. This drug is usedto prevent and treat osteoporosis as well as several other bone diseases. Alendronate Sodium is sold as tablets or solutions,alone or in combination with Vitamin D.METHODAnalytical ConditionsColumn: Anion-exchange,4.1x150 mm,catalog number 1441150Alendronate SodiumTemperature: 45 °CFlow Rate: 1.0 mL/minMobile Phase: Sodium Nitrate, 50 mM,pH 7.8 / Methanol (60:40)Post-column ConditionsPost-column System: Pinnacle PCXHeated Reactor Volume: 0.5 mLReactor Temperature: 70 °CReagent: OPA (300 mg), Thiouor (2g),30 % Brij 35 (3 mL) in 950 mL of OD104Reagent Flow Rate: 0.5 mL/min0 5 10Chromatogram of Fosavance® tablet(Alendronate sodium 70 mg, Vitamin D 2800 IU)min47Detection: FLD ex: 330 nm, em: 465 nmSample PreparationCrush 1 tablet and mix with water. Use enough water to getAlendronate Sodium concentration between 300 and 1000µg/mL. Heat the solution using a water bath to 65 ºC andstir for 30 min. Filter liquid portion through 0.45 µm lter.Put ltrate in HPLC autosampler vial and inject 10 µL.ALENDRONIC ACID ANALYSIS CATALOG INFORMATIONCATALOG NO.DESCRIPTION1441150 Anion-exchange column, (4.1 x 150 mm), 5 µmPRODUCT CATALOGPICKERING LABORATORIES10/11

“PURE CHEMICALS DO WHAT THEY ARE SUPPOSED TO EVERY TIME.”To guarantee our formulations, we purify all the reagents that go into them, do analytical conrmationof purity, validate there are no anomalies in the base line and that the peaks are in the right place.REBECCA SMITH / RESEARCH CHEMISTDAVID MAZAWA / TECHNICAL SUPPORT CHEMISTAutomation is a key elementin reproducibility.

REAGENTS & CHEMICALSp-DIMETHYLAMINOBENZALDEHYDE (DMAB)CHROMATOGRAPHIC GRADE FOR POST-COLUMN ANALYSISOF SULFA DRUGS IN FEED AND ANIMAL TISSUESp-Dimethylaminobenzaldehyde (DMAB; Ehrlich’s Reagent)reacts rapidly with many primary amines to form acomplex with maximum absorbance at 450 nm.Reversed-phase LC followed by post-column derivatizationis used to determine the levels of Sulfamethazine,Sulfathiazole and related compounds in feeds, premixesand animal tissues. Although these compounds have a UVchromophore, so do most of the other constituents in thecomplex sample matrix. Forming a colored derivative postcolumnallows shifting of the detection wavelength into thevisible range thus greatly enhancing selectivity.p-DIMETHYLAMINOBENZALDEHYDE (DMAB) CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITY3700-0400 p-Dimethylaminobenzaldehyde (DMAB) each (5 g/bottle)THIOFLUORN,N-DIMETHYL-2-MERCAPTOETHYLAMINE HYDROCHLORIDE – CHROMATOGRAPHIC GRADEFOR AUTOMATED POST-COLUMN DERIVATIZATION OF PRIMARY AMINESPrimary amines form highly uorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptanunder basic conditions.The products of this reaction, 1-alkyl-2-thioalkyl-subsitituted isoindoles, exhibit optimal excitation at 330 nm andmaximal emission at 465 nm.Pickering’s Thiouor, a solid, nearly odorless nucleophile, is a superior substitute for 2-Mercaptoethanol in the preparationof OPA reagents. It forms a more stable reagent and a longer-lasting uorophore with OPA than does 2-Mercaptoethanol, yetit has the same uorescence properties.Unlike the volatile 2-Mercaptoethanol, Thiouor will not migrate through the gas manifold and regulator of the OPAreagent pressurization system.Two grams of Thiouor is equivalent to 1 mL of 2-Mercaptoethanol.51THIOFLUOR CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITY3700-2000 Thiofluor each (10 g/bottle)o-PHTHALALDEHYDE (OPA) DILUENTCHROMATOGRAPHIC GRADE FOR PREPARATION OF o-PHTHALALDEHYDE REAGENTSThe derivatization of primary amines with o-Phthalaldehyde and a mercaptan requires basic conditions. To ensure theoptimum pH of the reaction it is important to use the correct diluent.Three application specic diluents are available : CB910 and GA104 for carbamate and glyphosate analyses respectively, andOD104 for analysis of amino acids and other amines.These borate buffers are produced from starting materials which are free of heavy metals and amines. As with most otherproducts of Pickering Laboratories, the quality of the OPA Diluents is veried by actual post-column HPLC analysis.o-PHTHALALDEHYDE (OPA) DILUENT CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYCB910 o-Phthalaldehyde (OPA) Diluent for Carbamate analysis case of 4 (950 mL/bottle)GA104 o-Phthalaldehyde (OPA) Diluent for Glyphosate analysis case of 4 (950 mL/bottle)OD104 o-Phthalaldehyde (OPA) Diluent for amino acids and amines analysis case of 4 (950 mL/bottle)PRODUCT CATALOGPICKERING LABORATORIES10/11

REAGENTS & CHEMICALSSERAPREP & URIPREPCHROMATOGRAPHIC GRADE REAGENTS FOR THE PREPARATIONOF NATIVE SAMPLES FOR AMINO ACID ANALYSISPreparation of samples such as serum, urine, other physiologicaluids, plant extracts, foods and beverages requires controlof pH and normality, and removal of soluble protein.The samples must be held to a narrow pH range between2.1 and 2.5, and at the proper Lithium ion concentration toensure reproducibility in the early part of the chromatogram.SERAPREP and URIPREP replace commonly usedprotein precipitation reagents such as Acetonitrile, Perchloricacid, and Picric acid, and eliminate the need for dialysis,ultraltration, and the repeated centrifugation steps, followedby pH adjustment.SERAPREP is used for preparing serum and othersamples with high buffering capacity, e.g. sardine oil.URIPREP is used for urine and other samples with lowbuffering capacity, such as fruit juices, beer and wines. Theefciency of protein precipitation and need for post-centrifugationpH adjustment of the sample determine whichreagent is best for your particular sample.SERAPREP & URIPREP CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYSP100 SERAPREP each (250 mL/bottle)UP100 URIPREP each (250 mL/bottle)HYDROLYSIS REAGENT CB130CHROMATOGRAPHIC GRADEThis 0.05 M NaOH/C47 reagent is applied in the rststage of post-column carbamate derivatization. At 100 ºCthe separated carbamates are converted from urethanes toMethylamine. The Methylamine can then react with OPAand Thiouor to form the characteristically uorescentisoindole.Hydrolysis Reagent contains C47 which prevents theprecipitation of insoluble minerals (e.g. Ca 2+ , Mg 2+ )from samples of hard drinking water and ground water.The C47 additive complexes these minerals and keepsthem in solution.HYDROLYSIS REAGENT CB130.2CHROMATOGRAPHIC GRADEThe new Hydrolysis Reagent CB130.2 is formulated accordingto USEPA Method 531.2 and contains 0.075 MSodium Hydroxide. This reagent is applied during the rststage of post-column derivatization for the analysis of carbamatepesticides.Hydrolysis Reagent CB130.2 contains C47, which preventsthe precipitation of insoluble minerals from samplesof hard drinking and ground water, and protects yourinstruments from damage.HYDROLYSIS REAGENT CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYCB130 Hydrolysis Reagent for carbamateanalysis 0.05 Mcase of 4(950 mL/bottle)CB130.2Hydrolysis Reagent for carbamateanalysis 0.075 Mcase of 4(950 mL/bottle)

REAGENTS & CHEMICALSHYPOCHLORITE DILUENTCHROMATOGRAPHIC GRADE FOR THE PREPARATION OFTHE OXIDIZING REAGENT FOR POST-COLUMN GLYPHOSATEHERBICIDE ANALYSISThis diluent is used to prepare the oxidizing reagent requiredto convert glyphosate to a primary amine prior toreacting it with OPA. The reagent is prepared by adding100 µL of 5 % solution of Sodium hyphochlorite to one950 mL bottle of Diluent. The pH 11.6 Diluent is formulatedto ensure pH stability of the mixed stream of columnefuent and oxidizing reagent. This diluent could also beused in uorescence detection of other secondary amines.HYPOCHLORITE DILUENT CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYGA116 Hypochlorite Diluent for Glyphosate analysis case of 4(950 mL/bottle)53CHLORAC BUFFERCHROMATOGRAPHIC GRADE FOR PRESERVATIONOF AQUEOUS CARBAMATE SAMPLESTrace analysis of carbamate insecticide residues in drinkingwater is a mandated requirement in the U.S. Since severalof the common carbamates—Carbaryl, Oxamyl, Hydroxycarbofuran—arelabile in water due to oxidation or hydrolysis,the samples and standards must be preserved in order toobtain valid results.ChlorAC Buffer from Pickering Laboratories is a highlypuried Chromatographic Grade preservative. It is preparedfrom Monochloracetic acid and Potassium acetate toEPA specications. ChlorAC is guaranteed to be free ofco-eluting interferences for the analytes in EPA 531.1.CHLORAC BUFFER CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITY1700-0132 ChlorAC Buffer each (250 mL/bottle)PRODUCT CATALOGPICKERING LABORATORIES10/11

REAGENTS & CHEMICALSRESTORECHROMATOGRAPHIC GRADE FOR REMOVAL OF METAL ION CONTAMINATION AND RESTORATIONOF PROPER ION BALANCE IN GLYPHOSATE ANALYTICAL COLUMNS AND GUARDSGlyphosate herbicide analysis by post-column HPLC according to US EPA Method 547 employs a cation-exchange column.Many polyvalent metal ions which may be present in the sample, especially Iron, will accumulate in the guard or analyticalcolumn. As little as 100 nmole of Ferric Iron, for example, will cause serious degradation of column performance;larger amounts can actually cause the glyphosate peak to vanish completely.Glyphosate RESTORE rapidly removes Iron and all other polyvalent metals from the column and guard. In addition, itRESTORE CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITY1700-0140 RESTORE, removes metal ions from Glyphosate column and guard each (250 mL/bottle)PURE WATERPURE WATER CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITY1700-2300 Ultra High Pure Water 4 x 950 mL/bottleEXTRACTION SOLUTIONAMINOGLYCOSIDE EXTRACTION SOLUTION CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITY1750-1118 Aminoglycoside Extraction Solution 4 x 950 mL/bottleLi220 & Na220CHROMATOGRAPHIC GRADE SAMPLES AND STANDARDS DILUENTS FOR AMINO ACID ANALYSISUse of these diluents is essential to ensuring reproducibility from injection to injection. They establish a uniform pH and ionconcentration at the outset, regardless of the source and pre-treatment of the sample. The sample is maintained buffered andat optimum pH for sample storage and analysis.Use Na220 for hydrolysate samples and to dilute amino acid calibration standards for use with Sodium columns andbuffers. Use Li220 for native amino acid samples and calibration standards for use with Lithium columns and buffers.Li220 & Na220 CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYLi220 Lithium Diluent, pH 2.36 case of 4 (250 mL/bottle)Na220 Sodium Diluent, pH 2.20 case of 4 (250 mL/bottle)

REAGENTS & CHEMICALSCALIBRATION STANDARDS & TEST MIXTURES• Quantitative and guaranteed• Each lot tested chromatographically• Chromatographically-pure starting componentsPickering’s Amino Acid standards have a reputation worldwide for quality and reliability in all post-column systems andmethods. Six Amino Acid Standard mixtures are available for a variety of applications. Except for the three-component testmixture (1700-0070) the standards are in 5 mL vials, in an appropriate citrate buffer.Although they are stored frozen at the factory, Pickering’s calibration standards remain stable when shipped at ambienttemperatures. Upon receipt, however, it is important to place them into a freezer immediately, and store until ready for use.Test Mixtures are the qualitative standards and intended to be used to establish an elution prole and for troubleshooting.TEST MIXTURES CATALOG INFORMATIONCATALOG NO. DESCRIPTION CONSTITUENTS1700-0070 Amino Acid Test Mixture Concentration 0.25 µmole/mL in 0.01 NHCl, 1.5 mL L-Cysteic AcidL-ThreonineL-Serine1700-0063 Carbamate Test Mixture Concentration 2.5 µg/mL in Methanal, 1.5 mL AldicarbAldicarb SulfoneAldicarb SulfoxideDMC (4-Bromo-3,5-dimethylphenyl-N-methylcarbamate)CarbarylCarbofuran3-HydroxycarbofuranMethiocarbMethomyl1-NaphtholOxamylPropoxur1700-0080 Glyphosate Test Mixture Concentration 2.5 µg/mL in water, 1.5 mL GlyphosateAMPA (Aminomethyl-phosphonic acid)55CALIBRATION STANDARDS & CATALOG INFORMATIONCATALOG NO.DESCRIPTION011006P Native Sample Standard with Norleucine, in 0.2 N Lithium citrate buffer pH 2.36, 5 mL012006P Native Sample Standard without Norleucine, in 0.2 N Lithium citrate buffer pH 2.36, 5 mL1700-0150 Native Sample Standard for rapid-screen for PKU and MSUD, in 0.2 N Lithium citrate buffer pH 2.36, 5 mL012506C Collagen Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL012506H Protein Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL1700-0155 Oxidized Feed Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL1700-0170Native Sample Standard without Norleucine & Alpha-Amino-Beta-guanidinopropionic acid, in 0.2 N Lithium citratebuffer pH 2.36, 5 mL1700-0175 Native Sample Standard, basics, in 0.1 N HCl, 5 mL1700-0180 Native Sample Standard, acidics and neutrals, in 0.1 N HCl, 5 mLPRODUCT CATALOGPICKERING LABORATORIES10/11

REAGENTS & CHEMICALSCALIBRATION STANDARDS FOR AMINO ACID ANALYSISCONSTITUENTS 1700-0180* 1700-0175* 011006P 012006P 1700-0150 012506C 012506H 1700-0155 1700-0170Beta-Alanine • • • •Alanine • • • • • • •D,L-a-Amino-adipic acid • • • •Gamma-Amino butyric acid • • • •Alpha-Amino-n-butyric acid • • • •D,L,b-Amino-i-butyric acid • • • •Alpha-Amino-Betaguanidinopropionic• •acidAmmonia • • • • • • •Anserine • • • •Arginine • • • • • • •Asparagine • • • •Aspartic acid • • • • • • •Carnosine • • • •Citrulline • • • •Creatinine • • • •Cystathionine • • • •Cystine • (1.25) • • • • •Cysteic acid•Ethanolamine • • • •Glutamic acid • • • • • • •Glycine • • • • • • •Histidine • • • • • • •D,L-Homocystine • • • •L,L & allo-Hydroxylysine • • • • •4-trans-L-Hydroxyproline • • • • (1.25) •Isoleucine • • • • • • • •Leucine • • • • • • • •Lysine • • • • • • •Methionine • • • • • • •Methionine-D,L-sulfoxide•Methionine-D,L-sulfone•1-Methyl-histidine • • • •3-Methyl-histidine • • • •Norleucine•Ornithine • • • •Phenylalanine • • • • • • • •Phosphoethanolamine • • • •Phosphoserine • • • •Proline • • • • (1.25) • • •Sarcosine • • • •Serine • • • • • • •Taurine • • • •Threonine • • • • • • •Tryptophan • • • • •Tyrosine • • • • • • •Urea • • • •Valine • • • • • • •NOTE: Concentration of all the constituents in the Amino Acid standards is 0.25 µmole/mL unless otherwise specified.*Concentration of all the constituents is 2.5 µmole/mL unless otherwise specified

REAGENTS & CHEMICALSARTIFICIAL PERSPIRATIONFOR TESTING OF PRODUCT COLORFASTNESS, CORROSION, DISCOLORATIONThe articial Eccrine Sweat we offer is a stabilized, ready to use solution. It consists of the 19 amino acids, the seven mostabundant minerals and the four most abundant metabolites at a pH of 4.5. All concentrations closely match experimentallydetermined values for adult human Eccrine Sweat. Custom formulations are available on request.Store and HandlingThe stabilized solution is preserved with a fungicide and bactericide for one year shelf life.The non-stabilized product is kept frozen.METABOLITESMINERALSAMINO ACIDSARTIFICIAL ECCRINE PERSPIRATION LIST OF INGREDIENTSUric AcidUreaLactic AcidAmmoniaSodium Iron PhosphateCalcium Copper SulfateMagnesiumPotassiumZincChlorideGlycine L-Histidine L-Serine (Largest amount)L-Alanine L-Isoleucine L-ThreonineL-Arginine L-Leucine L-TyrosineL-Asparagine L-Lysine L-ValineL-Aspartic acid L-Methionine TaurineL-CitrullineL-OrnithineL-Glutamic acidL-Phenylalanine57ARTIFICIAL PERSPIRATION CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITY1700-0020 Artificial Eccrine Perspiration – stabilized 200 mL1700-0022 Artificial Eccrine Perspiration – not stabilized 200 mL1700-0024 Artificial Eccrine Perspiration – stabilized 5 mL**Ask for a free sampleNOTE: Custom pH formulations can be made in the pH range 3.0 to 9.0.For custom component formulations please contact support@pickeringlabs.com for quote.PRODUCT CATALOGPICKERING LABORATORIES10/11

ELUANTSCHROMATOGRAPHIC GRADEPickering Laboratories eluants are manufactured understrictly controlled conditions to guarantee purity, stabilityand consistency for a reproducible high quality chromatogram.This quality standard guarantees the resulting chromatogramwill be free of any noise and interference.The Lithium and Sodium eluants are not sensitive to oxidationand do not need refrigeration, either in storage or use.Degassing is not required. However, they should be protectedfrom air to prevent contamination. Ambient air actuallycontains amines and amino acids that will dissolve in thelow-pH eluants and will appear in the chromatograms.All buffers are packaged in cases of four 950 mL polyethylenebottles. The regenerants are packaged in single 950mL bottles because of the small volumes used during eachanalysis. Sample diluents are packaged in cases of four 250mL borosilicate glass bottles.CUSTOM ELUANTSWe develop and manufacture custom lots of Eluants forcustomers who require our “Guaranteed Chemistry” commitmentof purity and certied use. Let us make some foryou! Call for details.ELUANTS CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYAmino Acid Analysis, Sodium-Based Solutions:Na270 Sodium Eluant, pH 2.70 case of 4 (950 mL/bottle)Na315 Sodium Eluant, pH 3.15 case of 4 (950 mL/bottle)Na328 Sodium Eluant, pH 3.28 case of 4 (950 mL/bottle)Na425 Sodium Eluant, pH 4.25 case of 4 (950 mL/bottle)Na640 Sodium Eluant, pH 6.4 case of 4 (950 mL/bottle)Na740 Sodium Eluant, pH 7.40 case of 4 (950 mL/bottle)1700-0112* Sodium Eluant, pH 3.15,case of 4 (950 mL/bottle)with 5 % Sulfolane,1700-0114* Sodium Eluant, pH 3.33,case of 4 (950 mL/bottle)with 2.5 % Sulfolane,RG011 Sodium Column Regenerant each (950 mL/bottle)Na220 Sodium Diluent, pH 2.20, for calibrationcase of 4 (950 mL/bottle)standards/hydrolyzed samplesAmino Acid Analysis, Lithium-Based Solutions:Li275 Lithium Eluant, pH 2.75 case of 4 (950 mL/bottle)Li280 Lithium Eluant, pH 2.80 case of 4 (950 mL/bottle)Li292 Lithium Eluant, pH 2.92 case of 4 (950 mL/bottle)1700-1125 Lithium Eluant, pH 2.80 case of 4 (950 mL/bottle)Li357 Lithium Eluant, pH 3.57 case of 4 (950 mL/bottle)Li365 Lithium Eluant, pH 3.65 case of 4 (950 mL/bottle)Li375 Lithium Eluant, pH 3.75 case of 4 (950 mL/bottle)Li750 Lithium Eluant, pH 7.50 case of 4 (950 mL/bottle)RG003 Lithium Column Regenerant each (950 mL/bottle)Li220 Lithium Diluent, pH 2.36, for calibrationcase of 4 (250 mL/bottle)standards/prepared samplesGlyphosate Herbicide Analysis:K200 Glyphosate Potassiumcase of 4 (950 mL/bottle)Phosphate EluantRG019 Glyphosate Column Regenerant each (950 mL/bottle)Potassium-Based Solutions for ALKION Column for AminoglycosideAntibiotics, Paraquat/Diquat in Water Analysis & Streptomycin:1700-1101 Potassium Phosphate Eluant, KO1 case of 4 (950 mL/bottle)1700-1102 Potassium Hydroxide Eluant, KO2 case of 4 (950 mL/bottle)1700-1103 Potassium Chloride Eluant, KO3 case of 4 (950 mL/bottle)Biogenic Amines/Polyamines Analysis:K563 Potassium Phosphate Eluant, pH 5.63 case of 4 (950 mL/bottle)K600 Potassium Phosphate Eluant, pH 6.00 case of 4 (950 mL/bottle)K130 Potassium Column Regenerant each (950 mL/bottle)Muti-Residue Mycotoxin Analysis:1700-1108 Sodium Phosphate Eluant, pH 3.3 case of 4 (950 mL/bottle)* For use with 1154150 column with serial numbers after 1314

APPLICATION KITSAMINO ACID ANALYSIS KITSCATALOG NO.AMINO ACID ANALYSIS KITSDESCRIPTION0352-0061 30-MIN HIGH-EFFICIENCY COLLAGEN HYDROLYSATE KIT:1154110T30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700-0070 amino acid test mixture1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsNa220 Sodium Diluent, pH 2.20, 4 x 250 mLNa315 Sodium Eluant, pH 3.15, case of 4 ( 950 mL/bottle)Na740 Sodium Eluant, pH 7.40, 4 x 950 mLRG011 Sodium Column Regenerant, 950 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)012506C Sodium Calibration Standard, for collagen hydrolysate, 0.25 µmole/mL, 5 mL0352-0062 KIT IDENTICAL TO 0352-0061 WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)0352-0063KIT IDENTICAL TO 0352-0061 WITH ITEMS BELOWREPLACING T100C:OD104 OPA Diluent, 4 x 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)0352-0057 30-MIN HIGH-EFFICIENCY PROTEIN HYDROLYSATE KIT:1154110T30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700-0070 amino acid test mixture1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsNa220 Sodium Diluent, pH 2.20, 4 x 250 mLNa315 Sodium Eluant, pH 3.15, case of 4 ( 950 mL/bottle)Na740 Sodium Eluant, pH 7.40, 4 x 950 mLRG011 Sodium Column Regenerant, 950 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)012506H Sodium Calibration Standard, for protein hydrolysate, 0.25 µmole/mL, 5 mL0352-0058 KIT IDENTICAL TO 0352-0057 WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)0352-0059 KIT IDENTICAL TO 0352-0057 WITH ITEMS BELOW REPLACING T100C:OD104 OPA Diluent, 4 x950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)AT30SH STANDARD 60-MIN PROTEIN HYDROLYSATE KIT:1193250 Sodium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070)1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsNa328 Sodium Eluant, pH 3.28, 4 x 950 mLNa740 Sodium Eluant, pH 7.40, 4 x 950 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)RG011 Sodium Column Regenerant, 950 mLNa220 Sodium Diluent, pH 2.20, 4 x 250 mL012506H Sodium Calibration Standard, for protein hydrolysate, 0.25 µmole/mL, 5 mL0352-0030 KIT IDENTICAL TO AT30SH WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)AO30SH KIT IDENTICAL TO AT30SH WITH ITEMS BELOW REPLACING T100C:OD104 OPA Diluent, 4 x950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)AT31FH HIGH-EFFICIENCY 55-MIN PROTEIN HYDROYSIS KIT:1154150TSodium Ion-exchange Column, 4.0 x 150 mm (with test mixture1700-0070)1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsNa315 Sodium Eluant, pH 3.15, 4 x 950 mLNa740 Sodium Eluant, pH 7.40, 4 x 950 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)RG011 Sodium Column Regenerant, 950 mLNa220 Sodium Diluent, pH 2.20, 4 x 250 mL012506H Sodium Calibration Standard, for protein hydrolysate, 0.25 µmole/mL, 5 mL0352-0031 KIT IDENTICAL TO AT31FH WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)AO31FH KIT IDENTICAL TO AT31FH WITH ITEMS BELOW REPLACING A T100C:OD104 OPA Diluent, 4 x 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)AT32FC HIGH-EFFICIENCY 55-MIN COLLAGEN HYDROLYSATE KIT:1154150TSodium Ion-exchange Column, 4.0 x 150 mm (with test mixture1700-0070)1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsNa315 Sodium Eluant, pH 3.15, 4 x 950 mLNa740 Sodium Eluant, pH 7.40, 4 x 950 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)RG011 Sodium Column Regenerant, 950 mLNa220 Sodium Diluent, pH 2.20, 4 x 250 mL012506CSodium Calibration Standard, for collagen hydrolysate 0.25 µmole/mL, Proline and Hydroxyproline, 1.25 µmole/mL, 5 mL0352-0032 KIT IDENTICAL TO AT32FC WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)AO32FC KIT IDENTICAL TO AT32FC WITH ITEMS BELOW REPLACING T100C:OD104 OPA Diluent, 4 x 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)0352-0018 60-MIN OXIDIZED FEED HYDROLYSATE KIT:1154150T Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsNa270 Sodium Eluant, pH 2.70, 4 x 950 mLNa740 Sodium Eluant, pH 7.40, 4 x 950 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)RG011 Sodium Column Regenerant, 950 mLNa220 Sodium Diluent, pH 2.20, 4 x 250 mL1700-0155Sodium Calibration Standard, for oxidized feed hydrolysate, 0.25µmole/mL, 5 mL0352-0017 KIT IDENTICAL TO 0352-0018 WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)0352-0019 KIT IDENTICAL TO 0352-0018 W/ ITEMS BELOW REPLACING T100COD104 OPA Diluent, 4 x 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g, (2 each per kit)59PRODUCT CATALOGPICKERING LABORATORIES10/11

APPLICATION KITSAmino Acid Analysis Kits Continued0352-000670-MIN HIGH-EFFICIENCY PHYSIOLOGICFLUID/NATIVE SAMPLE KIT:0354675T Lithium Ion-exchange Column, 4.6 x 75 mm (with test mixture 1700-0170)1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDs1700-1125 Lithium Eluant, pH 2.80, 4 x 950 mLLi220 Lithium Diluent, pH 2.20, 4 x 250 mLLi365 Lithium Diluent, pH 3.65, 4 x 950 mLLi375 Lithium Diluent, pH 3.75, 4 x 950 mLRG003 Lithium Column Regenerant, 950 mLSP100 SERAPREP, 250 mLUP100 URIPREP, 250 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)0352-0007 KIT IDENTICAL TO 0352-0006 WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)0352-0008KIT IDENTICAL TO 0352-0006 WITH ITEMS BELOWREPLACING T100C:OD104 OPA Diluent, 4 x 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)AT33SP STANDARD 185-MIN PHYSIOLOGIC FLUID/NATIVE SAMPLE KIT:0393250 Lithium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070)1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsLi275 Lithium Eluant, pH 2.75, 4 x 950 mLLi750 Lithium Eluant, pH 7.50, 4 x 950 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)RG003 Lithium Column Regenerant, 950 mLLi220 Lithium Diluent, pH 2.36, 4 x 250 mL011006P Lithium Calibration Standard, with norleucine, 0.25 µmole/mL, 5 mLSP100 SERAPREP, 250 mLUP100 URIPREP, 250 mL0352-0033 KIT IDENTICAL TO AT33SP WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)AO33SP KIT IDENTICAL TO AT33SP WITH ITEMS BELOW REPLACING T100C:OD104 OPA Diluent, 4 x 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)0352-0015120-MIN HIGH-EFFICIENCY PHYSIOLOGICFLUID/NATIVE SAMPLE KIT:0354100TLithium Ion-exchange Column, 4.0 x 100 mm (with test mixture1700-0070)1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsLi275 Lithium Eluant, pH 2.75, 4 x 950 mLLi750 Lithium Eluant, pH 7.50, 4 x 950 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)RG003 Lithium Column Regenerant, 950 mLLi220 Lithium Diluent, pH 2.36, 4 x 250 mL011006P Lithium Calibration Standard, with norleucine, 0.25 µmole/mL, 5 mLSP100 SERAPREP, 250 mLUP100 URIPREP, 250 mL0352-0011 KIT IDENTICAL TO 0352-0015 WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)0352-0012KIT IDENTICAL TO 0352-0015 WITH ITEMS BELOWREPLACING T100C:OD104 OPA Diluent, 4 x 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g ( 2 each per kit)0352-0013TEMPERATURE GRADIENT 90 MIN PHYSIOLOGICFLUID/NATIVE SAMPLE KIT:0354100TLithium Ion-exchange Column, 4.0 x 100 mm (with test mixture1700-0170)1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDs1700-1125 Lithium Eluant, pH 2.80, 4 x 950 mLLi220 Lithium Diluent, pH 2.20, 4 x 250 mLLi365 Lithium Diluent, pH 3.65, 4 x 950 mLLi375 Lithium Diluent, pH 3.75, 4 x 950 mLRG003 Lithium Column Regenerant, 950 mLSP100 SERAPREP, 250 mLUP100 URIPREP, 250 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)0352-0014 KIT IDENTICAL TO 0352-0013 WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)0352-0016KIT IDENTICAL TO 0352-0013 WITH ITEMSBELOW REPLACING T100C:OD104 OPA Diluent, 4 x 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)AT35PKHIGH-EFFICIENCY 7-MIN PKUDETECTION/PHYSIOLOGIC FLUID KIT:0354050 Lithium Ion-exchange Column, 4.0 x 50 mm1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsLi357 Lithium Eluant, pH 3.57, 4 x 950 mLT100C TRIONE® Ninhydrin Reagent, 4 x 950 mL (3-month shelf life)RG003 Lithium Column Regenerant, 950 mLLi220 Lithium Diluent, pH 2.36, 4 x 250 mL1700-0150Calibration Standard, contains Ile, Leu, Met, Tyr, & Phe, 0.25 µmole/mL, 5 mL0352-0035 KIT IDENTICAL TO AT35PK WITH T200 REPLACING T100C:T200TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL(12-month shelf life)

APPLICATION KITSPESTICIDE & HERBICIDE ANALYSIS KITSCATALOG NO.PESTICIDE & HERBICIDE ANALYSIS KITSDESCRIPTION0352-0002 CARBAMATE PESTICIDES ANALYSIS KIT, 23+ COMPOUNDS:084025018ECG001CB130CB910O120Analytical Column, C 8, expanded resolution, 4.0 × 250 mm (withCarbamate Test Mixture 1700-0063)Guard Cartridge Holder with 3 guard cartridgesCarbamate Hydrolysis Reagent, 4 × 950 mLCarbamate OPA Diluent, 4 × 950 mLo-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)1700-0132 ChlorAC Buffer, 250 mL0352-0003 CARBAMATE PESTICIDES ANALYSIS KIT, FOR EPA METHOD 531.1:1846250Analytical Column, C 18, high resolution/capacity, 4.6 × 250 mm(with Carbamate Test Mixture 1700-0063)18ECG001 Guard Cartridge Holder with 3 guard cartridgesCB130 Carbamate Hydrolysis Reagent, 4 × 950 mLCB910 Carbamate OPA Diluent, 4 × 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)1700-0132 ChlorAC Buffer, 250 mL0352-0009 CARBAMATE PESTICIDES ANALYSIS KIT, FOR EPA METHOD 531.2:1846250Analytical Column, C 18, high resolution/capacity, 4.6 × 250 mm(with Carbamate Test Mixture 1700-0063)18ECG001 Guard Cartridge Holder with 3 guard cartridgesCB130.2 Carbamate Hydrolysis Reagent, 4 × 950 mLCB910 Carbamate OPA Diluent, 4 × 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)1700-0132 ChlorAC Buffer, 250 mL0352-0004CARBAMATE PESTICIDES ANALYSIS KIT, FOR AOACMETHOD 985.23:1846150Analytical Column, C18, rapid analysis/QC, 4.6 × 150 mm (withCarbamate Test Mixture 1700-0063)18ECG001 Guard Cartridge Holder with 3 guard cartridgesCB130 Carbamate Hydrolysis Reagent, 4 × 950 mLCB910 Carbamate OPA Diluent, 4 × 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)1700-0132 ChlorAC Buffer, 250 mL0352-0010GLYPHOSATE HERBICIDE ANALYSIS KIT, FOR EPA METHOD547, AOAC METHOD 991.08:1954150Cation-exchange Column, K form, 4.0 × 150 mm (with GlyphosateTest Mixture 1700-0080)1700-3102 Cation-exchange GARD assembly: Holder w/ 2 replaceable GARDsK200 Glyphosate Potassium Phosphate Eluant, 4 × 950 mLRG019 Glyphosate Column Regenerant, 950 mLGA104 Glyphosate OPA Diluent, 4 × 950 mLGA116 Glyphosate Hypochlorite Diluent, 4 × 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)1700-0140 RESTORE, 250 mLOTHER POST-COLUMN ANALYSIS KITSOTHER POST-COLUMN ANALYSIS KITSCATALOG NO.DESCRIPTION0352-0040 BIOGENIC AMINES/POLYAMINES ANALYSIS KIT:9410917 ALKION Cation-exchange Column, K form, 4.0 × 150 mm9493020 ALKION Guard Column, K form, 3.0 × 20 mm1100-0200 Flow Restrictor, 300 psi, 10 cmK563 Potassium Phosphate Eluant, pH 5.63, 4 × 950 mLK600 Potassium Phosphate Eluant, pH 6.00, 4 × 950 mLK130 Potassium Column Regenerant, 950 mLOD104 OPA Diluent, 4 × 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)0352-0041 AMINOGLYCOSIDE ANTIBIOTICS ANALYSIS KIT:9410917 ALKION Cation-exchange Column, K form, 4.0 × 150 mm9493020 ALKION Guard Column, K form, 3.0 × 20 mm1100-0200 Flow Restrictor, 300 psi, 10 cm1700-1101 Potassium Phosphate Eluant, KO1, 4 × 950 mL1700-1102 Potassium Hydroxide Eluant, KO2, 4 × 950 mL1700-1103 Potassium Chloride Eluant, KO3, 4 × 950 mLOD104 OPA Diluent, 4 × 950 mLO120 o-Phthalaldehyde, 5 g3700-2000 Thiofluor, 10 g (2 each per kit)1700-1118 Aminoglyside extraction solution 4 x 950 mL0352-0042 PARAQUAT/DIQUAT IN WATER ANALYSIS KIT:9410917 ALKION Cation-exchange Column, K form, 4.0 × 150 mm9493020 ALKION Guard Column, K form, 3.0 × 20 mm1100-0200 Flow Restrictor, 300 psi, 10 cm1700-1101 Potassium Phosphate Eluant, KO1, 4 × 950 mL1700-1102 Potassium Hydroxide Eluant, KO2, 4 × 950 mL1700-1103 Potassium Chloride Eluant, KO3, 4 × 950 mL0352-0053 MULTI-RESIDUE MYCOTOXIN KIT:1612124 MYCOTOX Reversed-phase Column, C 18, 4.6 × 250 mm18ECG001 Guard Cartridge Holder with 3 cartridgesOD104 OPA Diluent, 4 × 950 mLO120 o-Phthalaldehyde, 5 g1700-1108 Sodium Phosphate Eluant, pH 3.33700-2000 Thiofluor, 10 g (2 each per kit)0352-0050 AFLATOXINS ANALYSIS KIT:1612124 MYCOTOX Reversed-phase Column, C 18, 4.6 × 250 mm18ECG001 Guard Cartridge Holder with 3 cartridges0352-0051 POLYETHER ANTIBIOTICS KIT:2381750 Polyether Column, C 18, 4.6 × 250 mm18ECG001 Guard Cartridge Holder with 3 cartridges3700-2200 Vanillin, 30 g0352-0052 PARALYTIC SHELLFISH TOXINS KIT:0846150 P.S.P. column, C 8, 4.0 × 150 mm18ECG001 Guard Cartridge Holder with 3 cartridges61PRODUCT CATALOGPICKERING LABORATORIES10/11

BECKMAN 6300/7300 COLUMNS & CHEMICALSANALYTICAL COLUMNS & GUARDS CATALOG INFORMATIONCATALOG NO.0354100T1154150BDESCRIPTIONLithium Cation-exchange column, 4.0 × 100 mmSodium Cation-exchange column, 4.0 × 150 mm0352020 Lithium guard column, 2.0 × 20 mm1193020 Sodium guard column, 3.0 × 20 mmELUANTS CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYPhysiological Fluids And Native Samples, 3-Buffer Method:Li292 1 Lithium Eluant, pH 2.92 case of 4 (950 mL/bottle)1700-1125 2 Lithium Eluant, pH 2.80 case of 4 (950 mL/bottle)Li365 Lithium Eluant, pH 3.65 case of 4 (950 mL/bottle)Li375 Lithium Eluant, pH 3.75 case of 4 (950 mL/bottle)RG003 Lithium Column Regenerant each (950 mL/bottle)Protein Hydrolysate Samples, Classic 3-Buffer Method:Na328 3 Sodium Eluant, pH 3.28 case of 4 (950 mL/bottle)1700-0114 4 Sodium Eluant, pH 3.33 case of 4 (950 mL/bottle)Na425 Sodium Eluant, pH 4.25 case of 4 (950 mL/bottle)Na640 Sodium Eluant, pH 6.40 case of 4 (950 mL/bottle)RG011 Sodium Column Regenerant each (950 mL/bottle)1 To use with columns 0354100A2 To use with columns 0354100T3 For columns 1154150 with serial number 1313 and before4 For columns 1154150 with serial number 1314 and afterNOTE: Please contact Pickering to obtain information on 3-buffer method for CollagenHydrolysate Oxidized Feed samples, 5-buffer method for Physiological Fluids and NativeSamples and for Beckman System GoldREAGENTS & CHEMICALS CATALOG INFORMATIONCATALOG NO. DESCRIPTION QUANTITYT100 TRIONE® Ninhydrin Reagenteach (950 mL/bottle)(3-month* shelf life)T100C TRIONE® Ninhydrin Reagentcase of 4 (950 mL/bottle)(3-month* shelf life)T200 TRIONE® Two-part Ninhydrin Reagent(12-month* shelf life before mixing)to prepare case of 4(900 mL/bottle)Li220 Lithium Diluent, pH 2.36 case of 4 (250 mL/bottle)Na220 Sodium Diluent, pH 2.20 case of 4 (250 mL/bottle)SP100 SERAPREP each (250 mL/bottle)UP100 URIPREP each (250 mL/bottle)012006P Native Sample standard, in 0.2 N Lithium each (5 mL/bottle)citrate buffer pH 2.36 1011006P Native Sample standard, in 0.2 N Lithium each (5 mL/bottle)citrate buffer pH 2.36 2012506H Protein Hydrolysate standard, in 0.27 N each (5 mL/bottle)Sodium citrate buffer pH 2.20 3012506C Collagen Hydrolysate standard, in 0.27 N each (5 mL/bottle)Sodium citrate buffer pH 2.20 41700-0155 Oxidized Feed Hydrolysate standard, in each (5 mL/bottle)0.27 N Sodium citrate buffer pH 2.20 51700-0070 Amino Acid test mixture, in 0.01 N HCl 6 each (1.5 mL/bottle)1700-0170 Native Sample Standard in 0.2 N Lithium each (5 mL/bottle)citrate buffer, pH 2.36 71700-0175 Native Sample Standard, basics, in 0.1 N each (5 mL/bottle)HCl, 5 mL 81700-0180 Native Sample Standard, acidics and each (5 mL/bottle)neutrals, in 0.1 N HCl, 5 mL 8* From date of manufacture1 Without Norleucine, 0.25 µmole/mL2 With Norleucine, 0.25 µmole/mL3 0.25 µmole/mL4 0.25 µmole/mL, Proline & Hydroxyproline, 1.25 µmole/mL5 0.25 µmole/mL6 0.25 µmole/mL7 Without Norleucine & Alpha-Amino-Beta -guanidinopropionic acid, 0.25 µmole/mL8 2.5 µmole/mL

REPLACEMENT COMPONENTS, FITTINGS & TUBINGPINNACLE PCX FLOW DIAGRAM– TWO-PUMP SYSTEMNut: 1452-0118Ferrule: 1452-0117COLUMNTRANSDUCER 21452-01841452-0041R2FLUSHR1Nut: 1452-0180Ferrule: 1452-01811452-01892161452-01882103-0463PUMP 27130-00083102-90401452-01853VALVE 2452103-04631452-0041FILTER1452-0182Nut: 1452-0118Ferrule: 1452-0117HEATEDREACTORAMBIENTREACTOR1100-2927632102-0463Nut: 1452-0118Ferrule: 1452-0117TRANSDUCER 11452-00401452-00411452-01881452-0189Nut: 1452-0114Ferrule: 1452-01123102-9040Coupler: 3102-3064FILTER1452-01842162102-0463PUMP 137130-0008VALVE 15Nut: 1452-0113Ferrule: 1452-01154Check Valve: 3106-1007Nut: 1452-0226Ferrule: 1452-0112FROMHPLCWASTE1452-0185TO DETECTORNut: 1452-0118Ferrule: 1452-0117Union: 3102-3063PRODUCT CATALOGPICKERING LABORATORIES10/11

REPLACEMENT COMPONENTS, FITTINGS & TUBINGPINNACLE PCX FLOW DIAGRAM– ONE-PUMP SYSTEMNut: 1452-0118Ferrule: 1452-0117R1Nut: 1452-0180Ferrule: 1452-0181FLUSH1452-0182Nut: 1452-0118Ferrule: 1452-01172103-04631452-0040COLUMNFILTER 3102-90402102-0463HEATEDREACTOR1452-01871552-0017Coupler: 3102-3064Nut: 1452-0116Ferrule: 1452-0115TRANSDUCERNut: 1452-0118Ferrule: 1452-0117PUMP1452-018412 61452-00933 VALVE 1451452-0190Nut: 1452-0114Ferrule: 1452-0112Nut: 1452-0113Ferrule: 1452-0115Nut: 1452-0118Ferrule: 1452-0117Nut: 3101-0011Ferrule: 1452-0112Check Valve: 3106-1007Nut: 1452-0226Ferrule: 1452-0112FROMHPLC1452-0185WASTETO DETECTOR2102-0463Nut: 1452-0118Ferrule: 1452-0117Union: 3102-3063Back PressureRegulator: 3102-9025bold = kit

REPLACEMENT COMPONENTS, FITTINGS & TUBINGVECTOR PCX FLOW DIAGRAMfromHPLC ColumnHEATEDREACTOR2104-0210(PEEK Tubing)1452-0040(Liquid Manifold)3102-9040(Filter Frit)500 psi OverPressureRelief ValveAA3102-9040(Filter Frit)1452-0041(Filter Manifold)RESTRICTORBRESTRICTORAMBIENTREACTOR1100-2927PULSEDAMPENER3106-1256(Pulse DampenerRebuild Kit)PULSEDAMPENERtoDetectorA65to InjectorC3551-0070(Pressure Switch)500 psiBPUMP 13106-12543106-12553106-12573106-1259(Vector PumpComponents)PUMP 2fromPumpD E FCD E F2103-0463Reagent Reservoir & Cap AssembliesBOTTLEBOTTLEANut: 1452-0118Ferrule: 1452-0117CNut: 1452-0177Ferrule: 1452-0176ENut: 1452-0118Ferrule: 1452-0117BNut: 1452-0179Ferrule: 1452-0178DNut: 1452-0180Ferrule: 1452-0181F3106-1010PRODUCT CATALOGPICKERING LABORATORIES10/11

REPLACEMENT COMPONENTS, FITTINGS & TUBINGPCX 5200 FLOW DIAGRAMInjector Pre-column 3102-9042Filter (0.5 m)ThermostattedColumn and GuardHeatedReactorAmbient Reactor1100-2927AA500 psiPressure Switch1552-00023102-3031AADetector3102-3031LC Pump1452-0035Over-pressure reliefvalve (525 psi)3102-9013Post-columnPressure1452-0032Back-pressureRegulator (100 psi)3102-9025LC EluantsAnti-siphon Valves3102-9016RestrictorsWasteReagent Filters3102-9040ABypass Valves3104-0080Reagent Pressure1452-0033ReagentPump2WasteNut: 1452-0180Ferrule: 1452-0181Check Valve3106-1010Reagent Bottles& Cap AssembliesA Nut: 1452-0118Ferrule: 1452-0117

REPLACEMENT COMPONENTS, FITTINGS & TUBINGPINNACLE PCX REAGENT PUMPAND VALVE COMPONENTSCATALOG NO.1452-0122 Pinnacle PCX Pump Seal KitDESCRIPTION1352-0007 Pinnacle PCX Pump Cylinder, Ceramic 70 mL1452-0038 Pinnacle PCX Pump Assembly, 70 mL with motor1452-0093 Pinnacle PCX Valve Face Assembly, liquid end1452-0045 Pinnacle PCX Valve Assembly, Valve Face and motor1552-0017 Pinnacle PCX Pressure Tranducer Assembly1452-0202 Pinnacle PCX Valve Maintenance Kit (Seals and Tools)1452-0200 Pinnacle PCX Valve Seal Tool1452-0201 Pinnacle PCX Valve Seals Kit1100-0500 Pinnacle PCX Tubing Kit - Dual Pump System1100-0501 Pinnacle PCX Tubing Kit - Single Pump SystemVECTOR PCX REAGENT PUMP COMPONENTSCATALOG NO.DESCRIPTION3106-1254 Pump Check Valve Kit – PEEK (Inlet & Outlet)3106-1255 Seal Kit (Piston seal, Back-up O-ring, Diaphragm, Tool)3106-1256 Pulse Damper Rebuild Kit (Diaphragm, O-rings, Diaphragm seal, Sealtool, 4 Hex wrenches)3106-1258 Prime/Purge Valve – PEEK3106-1259 Head Kit, Vector Pump, (head, check valves, piston and seal)3106-1257 Piston, Vector Pump1452-0176 Ferrule, Peek 1/16 x 10-32 S/T, 5 each1452-0177 Nut, Peek Long 1/16 x 10-32 S/T, 5 each3106-1260 Prime Purge Seal Kit – PEEKPCX5200 REAGENT PUMPSWITH PISTON WASHCATALOG NO.DESCRIPTION1452-0012 Simplex Reagent Pump & Motor Assembly, 50/60 Hz, 120 V1452-0013 Simplex Reagent Pump & Motor Assembly, 50/60 Hz, 240 V1452-0010 Duplex Reagent Pump & Motor Assembly, 50/60 Hz, 120 V1452-0011 Duplex Reagent Pump & Motor Assembly, 50/60 Hz, 240 V1452-0016 Inert PEEK, Simplex Reagent Pump & Motor Assembly, 50/60 Hz, 120 V1452-0017 Inert PEEK, Simplex Reagent Pump & Motor Assembly, 50/60 Hz, 240 V1452-0014 Inert PEEK, Duplex Reagent Pump & Motor Assembly, 50/60 Hz, 120 V1452-0015 Inert PEEK, Duplex Reagent Pump & Motor Assembly, 50/60 Hz, 240 VNOTE: 240 V pumps CE certifi ed. Please contact Pickering for pumps without Piston WashPCX5200 REAGENT PUMP COMPONENTSCATALOG NO.DESCRIPTION3106-1330 Seal & Backup Ring3106-1310 Seal Kit, includes 2 seals, back-up rings, installation tool & hex wrench3106-1314 Inlet Check Valve Assembly3106-1316 Outlet Check Valve Assembly3106-1318 Check Valve Cartridge, inlet or outlet3106-1332 Sapphire Piston, for piston-wash3106-1333 O-ring, for piston-wash3106-5174 Compression Ring3106-1322 Piston Guide/Retainer3106-1324 Liquid End Assembly3106-1326 Preventive Maintenance Kit, includes 2 check-valve cartridge repairkits & 3106-1310 items3106-1335 Seal Kit PEEK pump3106-1336 Check Valve Rebuild Kit3106-1337 Check Valve, Inlet PEEK3106-1338 Check Valve, Outlet PEEK3106-1339 Liquid End Assy, PEEK1100-4001 Piston-Wash Upgrade Kit3106-1313 Washer, Piston Seal Backup3106-1320 Piston, Sapphire no piston-wash3106-1342 Spring, Pump Piston WashNOTE: Please contact Pickering for pumps without Piston WashPINNACLE PCX REPLACEMENTREACTOR CARTRIDGECATALOG NO.DESCRIPTION1452-0094 Reactor Cartridge Coil Assembly - 0.15 mL 130 °C1452-0064 Reactor Cartridge Coil Assembly - 0.5 mL 130 °C1452-0095 Reactor Cartridge Coil Assembly - 1.0 mL 130 °C1452-0096 Reactor Cartridge Knitted Assembly - 1.4 mL 130 °C1452-0097 Reactor Cartridge Knitted Assembly - 2.0 mL 80 °C1452-0098 Reactor Cartridge Knitted Assembly - 2.8 mL 80 °C1452-0099 Reactor Cartridge Knitted Assembly - 3.0 mL 80 °C1452-0100 Reactor Cartridge Knitted Assembly - 1.2 mL & 1.6 mL 130 °C67PRODUCT CATALOGPICKERING LABORATORIES10/11

REPLACEMENT COMPONENTS, FITTINGS & TUBINGVECTOR PCX SUBSTITUTE REACTORVOLUMES OR REPLACEMENT REACTORSCATALOG NO.DESCRIPTION1452-01621452-01631452-01641452-01651452-01661452-01671452-01741452-01751100-2927 OPA Ambient Reactor, 0.010 in ID TFE Tubing1100-0282 Coil Assembly, 0.15 mL without Electronics1100-0281 Coil Assembly, 0.5 mL without Electronics1100-0283 Coil Assembly, 1.0 mL without ElectronicsNOTE: Custom volumes are availablePCX5200 SUBSTITUTE REACTOR VOLUMESOR REPLACEMENT REACTORSCATALOG NO.DESCRIPTION1100-3403 Reactor Heater & Coil Assembly, 0.1 mL, 150 °C max, 120 V1100-3404 Reactor Heater & Coil Assembly, 0.1 mL, 150 °C max, 240 V1100-34011100-34021100-26541100-26531100-26601100-26611100-34111100-34121100-26591100-26581100-35111100-35121100-26831100-26841100-26811100-26821100-35141100-3513Reactor Heater & Coil Assembly, 0.5 mL 150 °C max, 120 VReactor Heater & Coil Assembly, 0.5 mL 150 °C max, 240 VReactor Heater & Coil Assembly, 1.0 mL 150 °C max, 120 VReactor Heater & Coil Assembly, 1.0 mL 150 °C max, 240 VReactor Heater & Knitted Assembly, 1.4 mL 150 °C max, 120 VReactor Heater & Knitted Assembly, 1.4 mL 150 °C max, 240 VReactor Heater & Knitted Assembly, 1.2 & 1.6 mL 150 °C max, 120 VReactor Heater & Knitted Assembly, 1.2 & 1.6 mL 150 °C max, 240 VReactor Heater & Coil Assembly, 0.15 mL, 150 °C max, 120 VReactor Heater & Coil Assembly, 0.15 mL, 150 °C max, 240 VReactor Heater & Coil Assembly, 0.2 mL, 150 °C max, 120 VReactor Heater & Coil Assembly, 0.2 mL, 150 °C max, 240 VReactor Heater & Coil Assembly, 0.5 mL, 150 °C max, 120 VReactor Heater & Coil Assembly, 0.5 mL, 150 °C max, 240 VReactor Heater & Coil Assembly, 1.0 mL, 150 °C max, 120 VReactor Heater & Coil Assembly, 1.0 mL, 150 °C max, 240 VReactor Heater & Knitted Assembly, 1.4 mL, 150 °C max, 120 VReactor Heater & Knitted Assembly, 1.4 mL, 150 °C max, 240 VReactor Heater & Knitted Assembly, 2.0 mL, 150 °C max, 120 VReactor Heater & Knitted Assembly, 2.0 mL, 150 °C max, 240 VReactor Heater & Knitted Assembly, 2.8 mL, 80 °C max, 120 VReactor Heater & Knitted Assembly, 2.8 mL, 80 °C max, 240 VReactor Heater & Knitted Assembly, 3.0 mL, 80 °C max, 120 VReactor Heater & Knitted Assembly, 3.0 mL, 80 °C max, 240 VReactor Heater & Dual-knitted Assembly, 1.2 & 1.6 mL, 150 °C max, 120 VReactor Heater & Dual-knitted Assembly, 1.2 & 1.6 mL, 150 °C max, 240 V1100-2927 OPA Ambient Reactor, 0.010 in. ID TFE Tubing1100-0282 Coil Assembly, 0.15 mL without Electronics1100-0281 Coil Assembly, 0.5 mL without Electronics1100-0283 Coil Assembly, 1.0 mL without Electronics1100-0285 Coil Assembly 0.1 mLPANELS FOR PCX5200CATALOG NO.DESCRIPTION1452-0023 Single-reagent Post-column Gauge Panel1452-0020 Dual-reagent Post-column Gauge Panel1452-0027 Inert PEEK, Single-reagent Post-column Gauge Panel1452-0028 Inert PEEK, Dual-reagent Post-column Gauge Panel1452-0021 Reagent 1 Panel, for OPA, Hypochlorite & Hydrolysis Reagent1452-0022 Reagent 2 Panel, for OPA Reagent1452-0024 Reagent Panel, TRIONE® Ninhydrin Reagent1452-0025 Reagent 1 Panel, Inert PEEK flowpath1452-0026 Reagent 2 Panel, Inert PEEK flowpathREAGENT RESERVOIRS & CAP ASSEMBLIESCATALOG NO.DESCRIPTION3107-0137 Reservoir Bottle, safety-coated, 1L3107-0145 Reservoir Bottle, safety-coated, 2L3107-0146 Reservoir Bottle, safety-coated, 5L3107-0300 Reservoir Assembly, includes 1L bottle, and cap with integrated valve3107-0147 Cap Assembly for 1L bottle, includes cap with integrated valve3107-0148 Cap Assembly for 2L bottle, includes cap with integrated valve3107-0138 Reservoir Cap1925-0129 Cap for Flush Bottle GL-38, Black, Pinnacle PCX1925-0130 Cap for Wash Bottle GL-38, Black, Vector PCX & Pinnacle PCX1452-0120 Bottle, Piston Wash with cap, Pinnacle PCX & Vector PCX1452-0121 Bottle, System Flush with Cap, Pinnacle PCXNOTE: Tubing, nuts and ferrules sold separatelyPRESSURE REGULATORS,VALVES & GAUGESCATALOG NO.DESCRIPTION1152-0010 Gas Manifold & 5 psi Regulator Assembly, PCX 52001552-0002 Pressure Transducer Assembly, PCX 52003102-9025 Pressure Regulator, 100 psi, 1/4-28 connection, for detector waste,PCX 5200, Vector PCX and Pinnacle PCX3102-9013 Pressure Relief Valve, 525 psi, PCX 52003102-9016 Reagent Anti-siphon Valve, 60 psi, PCX 52003104-0080 Prime/Purge Valve Assembly, PCX 52003104-0081 Seal Kit for prime/purge valve, PCX 52001452-0032 Pressure Gauge Assembly, 0–1000 psi, PCX 52001452-0033 Pressure Gauge Assembly, 0–3000 psi, PCX 52001452-0034 Pressure Gauge Assembly, 0–1000 psi, for PEEK, PCX 52003102-9015 Back Pressure Regulator, 40 psi Non-Metal, PCX 52001452-0141 Gas Manifold Assembly, Vector PCX and Pinnacle PCX3106-1007 Inline Check Valve, Pinnacle PCX3106-1010 Check Valve, Reagent Cap

REPLACEMENT COMPONENTS, FITTINGS & TUBINGTEES & UNIONSFLOW RESTRICTORSCATALOG NO.FILTERS & FRITSCATALOG NO.DESCRIPTION1100-0200 Flow Restrictor, for OPA, Hypochlorite & Hydrolysis reagent, 300 psi@ 0.3 mL/min, 10 cm1100-0141 Flow Restrictor, TRIONE® Ninhydrin reagent, 65 psi @ 0.3 mL/min, 6 cm1100-0143 Inert PEEK Flow Restrictor, 550 psi @ 0.3 mL/min, 10 cm1100-0142 Flow Restrictor, 100 psi @ 0.3 mL/min, 10 cm1100-0205 Flow Restrictor, 200 psi @ 0.3 mL/min, 6 cm1100-0263 Flow Restrictor, 550 psi @ 0.3 mL/min, 17 cm1100-0262 Flow Restrictor, 1150 psi @ 0.3 mL/min, 20 cm1100-0300 Static Mixer, internal volume 800 µLNOTE: Listed pressures are for a fl ow rate of 0.3 mL/min at ambient temperaturewith 100 % water and pulse-dampened flow.DESCRIPTION3102-9032 Pre-column Filter Assembly, PCX 52003102-9042 Replacement Frit 0.5 µm for 3102-90323102-9036 Reagent Filter Assembly, PCX 52003102-9040 Replacement Reagent 10 µm Frit3102-9132 Filter Frit, 2 µm Reagent Filter3102-9047 Filter Frit, 0.5 µm PEEK3102-9030 In-Line FilterCATALOG NO.DESCRIPTION3102-3031 Tee, Valco type, 0.010 holes, 1/16"1352-0004 Tee Mount3102-4040 Adapter, 1/4 FPT, 1/16"3102-3050 Union, 0.020 holes, 1/16"3102-3051 Union, 0.050 holes, 1/16"3102-3053 Union, Bulkhead 1/4" x 283102-3054 Union, Bulkhead 1/16" x 0.0103102-3036 Tee, Peek, 1/16"3102-3044 Cross, PEEK 1/16" x 0.010"1452-0040 Liquid Manifold with relief Valve Assembly, Vector PCX & Pinnacle PCX1452-0041 Liquid “T” Manifold Assembly, Vector PCX & Pinnacle PCXTUBING, PLASTICCATALOG NO.DESCRIPTION2101-0006 TFE Tubing, 1/16” OD × 0.006” ID, 90 cm2101-0212 TFE Tubing, 1/16" OD × 0.010" ID, 90 cm2101-0216 TFE Tubing, 1/16" OD x 0.016" ID, 90 cm2101-0220 TFE Tubing, 1/16" OD x 0.020" ID, 90 cm2101-0225 TFE Tubing, 1/16" OD × 0.025" ID, 90 cm2104-0105 PEEK Tubing, 1/32 x 0.005" ID, 90 cm2104-0205 PEEK Tubing, 1/16 OD x 0.005", 90 cm2104-0210 Inert PEEK Tubing, 1/16" OD × 0.010" ID, 90 cm2104-0220 Inert PEEK Tubing, 1/16" OD × 0.020" ID, 90 cm1452-0035 PEEK Tubing Assembly, 0.007" ID × 1/32" (includes nut & ferrules)2102-0463 FEP (clear) Tubing, 1/8" OD × 0.063" ID, 90 cm2103-0463 SARAN® Tubing, 1/8" OD × 0.063" ID, 90 cm1100-2731 Waste Tube PCX 52002101-0231 C-flex Tubing, 1/8" OD × 1/16" ID, 90 cm69PRODUCT CATALOGPICKERING LABORATORIES10/11

REPLACEMENT COMPONENTS, FITTINGS & TUBINGTUBING, TYPE 316 STAINLESS STEELCATALOG NO.DESCRIPTION3103-1005 SST Tubing, 1/16" OD × 0.010" ID, 5 cm3103-1010 SST Tubing, 1/16" OD × 0.010" ID, 10 cm3103-1030 SST Tubing, 1/16" OD × 0.010" ID, 30 cm3103-3005 SST Tubing, 1/16" OD × 0.030" ID, 5 cm3103-3010 SST Tubing, 1/16" OD × 0.030" ID, 10 cm3103-3020 SST Tubing, 1/16" OD × 0.030" ID, 20 cm3103-3030 SST Tubing, 1/16" OD × 0.030" ID, 30 cmFITTINGS, PLASTICCATALOG NO.DESCRIPTION1452-0180 Nut, 1/4-28, 1/8" (Polypropylene) - Pack of 51452-0181 Ferrule, 1/4-28, 1/8" (Tefzel) - Pack of 53101-0007 Nut, 1/4-28, 1/16" (Kel-F)3101-0008 Ferrule, 1/4-28, 1/16" (Tefzel)3102-1110 Nut, 1/4 -28, 1/16" (Tefzel)3102-2110 Ferrule, 1/4-28, 1/16" (Tefzel)3101-0020 Plug (Male), 1/4-28 (Delrin)3101-0030 Plug (Male), 10-32, 1/16" (Delrin)3101-0060 Nut, Fingertight, 1/16" (Kel-F)1452-0118 Nut, 1/16 (PEEK), Lite Touch - Pack of 51452-0117 Ferrule, 1/16 (PEEK), Lite Touch - Pack of 53102-9160 Removal Tool, for Lite-Touch Ferrule3102-1302 Nut, Male 1/4-28 1/16 tubing SST3102-2108 Ferrule, 1/4" SST3101-0011 Nut, Female PEEK 1/4-281452-0112 Ferrule, 1/16 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 51452-0113 Nut, Short, 1/8 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 51452-0114 Nut, Short, 1/16 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 51452-0115 Ferrule, 1/8 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 51452-0116 Nut, Long, 1/8 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 51452-0226 Nut, Long, 1/16 x 1/4-28 (PEEK), Pinnacle PCX - Pack of 5FITTINGS, TYPE 316 STAINLESS STEELCATALOG NO.DESCRIPTION1452-0179 Nut (Male), Parker type, 10-32, 1/16" - Pack of 51452-0178 Ferrule, Parker type, 1/16" - Pack of 53102-1402 Nut (Male), Valco type, 10-32, 1/16"3102-2402 Ferrule, Valco type, 1 /16"3102-1404 Nut, Valco type, 1/32"3102-2404 Ferrule, Valco type, 1/32"

REPLACEMENT COMPONENTS, FITTINGS & TUBINGFUSES & MISC. ACCESSORIESCATALOG NO.DESCRIPTION3543-0045 Fuse, for 120 V instruments, PCX 52003543-0044 Fuse, for 240 V instruments, PCX 52003543-0010 Fuse, for power supply (PCB), PCX 52003543-0014 Fuse, 3.15A Time Delay3551-0073 Fuse, 5A Time Lag Highbreak, ceramic, Pinnacle PCX3107-0020 Syringe, 20 cc3102-9161 Tubing Cutter, for 1/8–1/16 in. tubing1100-0791 Plug, 1/16" 10-32 Male SST1100-4020 System Connection Harware Kit1100-4022 Column Fittings Kit, 1/16" SST1452-0029 Heat-Exchanger for Reactor3102-3064 Coupler for Guard and Column1452-0185 Pinnacle PCX , Tubing Assembly, Valve-Pump (2 each)1452-0184 Pinnacle PCX, Tubing Assembly, Pump-Transducer (2 each)1452-0183 Pinnacle PCX, Tubing Assembly, Column out –Manifold, Dual pump system1452-0182 Pinnacle PCX, Tubing Assembly, Column out –Manifold, Single pump system1452-0186 Pinnacle PCX & Vector PCX, Tubing Assembly, Gas Manifold-Bottle1452-0187 Pinnacle PCX, Tubing Assembly, Valve-Manifold, Single pump system1452-0188 Pinnacle PCX, Tubing Assembly, Transducer – Valve (2 each)1452-0189 Pinnacle PCX, Tubing Assembly, Valve Manifold (2 each)Dual pump system1452-0190 Pinnacle PCX, Tubing Assembly, Transducer –Valve, Single pump system0101-0008 Manual, Pinnacle PCX operations0101-0009 Manual, Vector PCX operations3560-2000 Cable, RS232, 10’1452-0126 Relay Cable Assembly1552-0033 Enable, Switch Vector71The following trademarks are the property of the companies listed:ALKIONBrij-35ChlorACChromatographic GradeHydrolysis Reagent C47RESTORESaranSeraprepTefzelThiofluorTRIONEUriprepVespelWindowsPickering Laboratories, Inc.ICI Americas, Inc.Pickering Laboratories, Inc.Pickering Laboratories, Inc.Pickering Laboratories, Inc.Pickering Laboratories, Inc.Dow Chemical CorporationPickering Laboratories, Inc.E.I. DuPont CorporationPickering Laboratories, Inc.Pickering Laboratories, Inc.Pickering Laboratories, Inc.E.I. DuPont CorporationMicrosoft CorporationPRODUCT CATALOGPICKERING LABORATORIES10/11

“THE LCTECH STORY”Pickering Laboratories’ Partner in GermanyMICHAEL V. PICKERING, PH.D. / FOUNDERWith the onset of a new decade, Pickering Laboratories is embarking on a major expansion of our product offerings:the core of which are GPC Sample clean-up instruments and Mycotoxin analysis products. These products areoutstanding in performance, ruggedness, and value. Better yet, they come from LCTech, a company we know verywell and have grown in business with.I chose to start this business and develop gradient Amino Acid Analysis using modern HPLC equipment back in1984. Nine years later, about the same time Pickering Labs decided to begin selling internationally, Michael Baumannleft Beckman Instruments in Germany to start LCTech GmbH and our synergistic relationship began.LCTech, now our exclusive European distributor, went on to develop GPC sample clean-up instruments as well asImmunoafnity products, primarily clean-up columns and Elisa kits for Mycotoxins. LCTech GmbH has had remarkablesuccess internationally, due in no small part to the fact that their products have the same chemistry, integrity, andfocus on customer needs that also characterize the Pickering Laboratories products. So when we decided to broadenthe Pickering Laboratories product line, it was an easy choice to partner with LCTech and become their exclusivedistributor in the United States and Canada.Since our introduction of LCTech products, we have found that our customers are realizing the quality of these productsand have become our best testimonials of performance and reliability, not to mention the improvement in theirday-to-day workloads.The future of Pickering Laboratories looks promising and we are proud to say that, along with Pickering products,LCTech products are worthy of our esteemed ‘Guaranteed Chemistry’ pledge.

LCTECH / GPC PRODUCT LINEGPC ULTRAAUTOMATED GPC SAMPLE CLEAN-UPGel permeation chromatography is a well-known technology for the removal of fats, proteins, and other large bio-moleculesfrom samples in order to prevent interference in the analysis of chlorinated & organo-phosphorous pesticides, PCBs,polyaromatic hydrocarbons (PAH), and other semi-volatile compounds.The GPC ULTRA automates Gel Permeation Chromatography (GPC) technology and sample concentration for theclean-up of complex animal tissues, food products, environmental, and many other types of samples. The high recoveriesand concentrated samples are easily analyzed by GC-ECD, GC-MS, LC-MS, etc.Ideally suited for high-throughput laboratories requiring reliability and reproducibility, the GPC ULTRA is optimized forunattended operation, low maintenance, and consistent recoveries.73FEATURE HIGHLIGHTS• Gel Permeation Chromatography With Automated Concentration• Automated Rinsing Eliminates Carry-Over Contamination• Automatically Cleans Up To 52 Samples Per Batch• Reproducible High Recoveries• Variety Of Collection And Sample Vial Options• Keypad Or PC Control• Sealed Sample VialsPOPULAR APPLICATIONS INCLUDE• Pesticides And Polychlorinated Biphenyls (PCB) – EN 1528, EPA 8082a• Pesticide Residues – EN 12393, DFG 519• Polycyclic Aromatic Hydrocarbon (PAH) – EPA 8310• Organochlorine Pesticide Residues – AOAC 984.21• Phthalates – EPA 8270c• Gel Permeation Clean-up – EPA 3640a• …And Many More, Including USGS And USDA MethodsPRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / GPC PRODUCT LINEGPC ULTRA PROCESSThe GPC components include an autosampler, precisionpump and valve injection system, a sample loop, anda matched GPC column. The concentration portion iscentered on a uniquely designed evaporation chamber supportedby vacuum and chiller hardware. The optional PCsoftware controls and monitors the operations and enableseach individual sample requirements programmed in foroptimal recoveries.AUTOMATED PROCESSES• Injection Of The Sample (26 Or 52 In Series) Via RobotNeedle And Sample Loop Onto The GPC Column• Clean-up Of The Sample Via Gel PermeationChromatography (GPC)• Transfer Of The Main Fraction Into A Laser ControlledVacuum Chamber• Online Concentration Of The Column Eluate Under VacuumWith Final Volume Controlled To A Precise 5 mL Or 2 mL WithInternal Standard• Aliquotation In Up To Three Vials• Rinse of Needle Loop, Ports, Vacuum Chamber, And All Tubing;Eliminating Carry-overGEL PERMEATION CHROMATOGRAPHY PROCESSESThe autosampler has multiple rack congurations forexibility in sample processing. Sample vials are availablewith a patented septum technology that, together with thesuperior injection needle design, virtually eliminates solventloss. The sample loop is designed for precise sample size andrinsing to eliminate carry-over. Using the optimized GPCcolumn from LCTech provides consistency and reliabilityfor the separation function.RotatingFlaskUpper LaserLower LaserCONCENTRATION PROCESSESAt the heart of the GPC ULTRA is a specially designedsealed evaporation chamber that is laser controlled andextremely accurate. Utilizing the best features of rotary vacuumevaporation designs, the chamber has been optimizedfor accurate automated measurement of nal sample.This concentration/evaporation feature can also be used asa stand alone process without the GPC clean-up.The evaporation chamber is designed with a 2 mL reductionchannel in the bottom of the chamber. This uniquefeature allows the lasers to measure across the small diameterchannel very accurately. The lasers use a proprietaryalgorithm to precisely identify the correct solution level.AUTOMATED CONCENTRATOR• Concentration Of Any Volume (250 mL) To A Precise FinalVolume Of 5 mL Or 2 mL W/ Internal Standard• Single Or Multiple Solvent Exchange Options/Settings• Quantitative Transfer Of The Concentrate Into The Initial Vial ForFurther Clean-up Purposes• Concentration To Dryness And Subsequent Dissolution• Dilution Of The Concentrate• Aliquotation Of The Concentrate Into Sealed Vials• Process For Samples With Internal Standards• Speeds Up Processes For A Faster Sample Preparation• Solvent Resistant Flow Path Of Glass, PTFE , Stainless SteelSYSTEM COMPONENTS• LCTECH GPC COLUMNThe specially designed GPC column is DIN-compliant filled withS-X3 Bio-Beads, 200-400 mesh. The column construction isglass and PTFE, 1/8” PTFE inlet and outlet, rigid PTFE base andadjustable plunger, sturdy construction, and pressure stable.• TOUCH-PAD CONTROL WITH LCD DISPLAYA four lined display with touch-pad input enables the system tooperate in a stand alone mode. The menu driven input isself-explanatory and intuitive for system operation.• SAMPLE HANDLING SYSTEMA precise swivel robot arm, in combination with a spring mountedneedle loop and an inert 10 mL syringe, transfers the liquid withinthe system. There are four solvent paths possible that allow forapplication flexibility and efficient system rinsing functions. Thesample loop is set to 5 mL (other volumes are availableupon request).HeatingBathExtremely accurate, sealed evaporationchamber with laser control.

LCTECH / GPC PRODUCT LINE• VACUUM CHAMBER WITH VACUUM PUMPIn a heated rotation vacuum chamber, samples are concentratedto a defined final volume and transferred into vials after the GPCstep or clean-up. Concentration of samples without GPC is possiblein the stand alone mode.• Sample concentration to a specific volume is controlled bylasers and software algorithms to identify the precise volume.A frequency-modulated membrane vacuum pump provides theoptimal performance with virtually silent operation.• SAMPLE RACKS & VIALSRacks for 26 and 52 positions for flexible sample and GC vialcombinations. With the 26-position rack, a concentrated samplecan be transferred into two GC vials and one 4 mL vial. With the52-position rack, the aliquot is placed in a 4 mL vial. For concentrationtasks, the system can be loaded with up to 52 positionswith 60 mL, 26 positions with 110 mL, or 12 positions with 250mL. For the concentrate, there are 1 mL autosampler vials or 16mL vials available. Sample vials and receptacles for fractions canbe sealed with septa (not required) to prevent sample or solventvapors from escaping.• GPC PUMPThe system contains a robust PLC-controlled double piston preparativepump adjustable from 0.04 – 40.00 mL/min. Preparativepump technology is known for longevity and reliability in continuousoperation environments. The pump flow rate is adjustablewith pulsation less than 1.0 %. With automatic high/low pressureshut-off and inert wetted components, the reliability and low maintenanceare superior to HPLC pump implementations.• CHILLERAn optimized high-performance chiller for the GPC ULTRA enablesvirtually complete solvent recovery in the condenser. Flexibilityof applications is possible with adjustable temperature control.• SOFTWARE VARIOSOFT (OPTIONAL)The LCTech PC Windows VarioSoft provides Laboratory InformationSystem compliance. The Graphical user interface is modeledafter most HPLC and GC software for familiar and intuitive controlof the operations. The Sample ID and Job List Sequence areencrypted and stored to meet all requirements for data security.• FIXED WAVELENGTH DETECTOR (OPTIONAL)The UV detector is a 254 nm fixed wavelength detector controlledby the VarioSoft GPC ULTRA software. It is used to monitor thequality of the GPC columns.TECHNICAL DATA OF THE GPC ULTRA SAMPLE CLEAN-UP SYSTEMAmbient Temperature (Operational) 10 – 40 °CRelative Humidity (Operational) 0 – 95 %ELECTRONIC DATAPower Supply 230 V ± 10 % or 120 V ± 10 %Communication InterfaceRS 232 for Terminal and PC controlModem for remote serviceVACUUM UNITMax. Suction PerformancePressure RangeGPC PUMPTypeFlow RatesPressure0.8 M3 / hAtmosphere – 2 mbarDual PistonAnalytical: 0.10 – 9.95 mL/minPreparative: 0.40 – 40.00 mL/min40 bar, InstrumentMEASURES & WEIGHTMeasures (W X D X H)750 X 600 X 1000 mmWeight (Operational)Ca. 110 kgCONCENTRATORTemperature Range Ambient – 70 ºCMax Evaporation Rate10 mL/minGPC ULTRA CATALOG INFORMATIONCATALOG NO.DESCRIPTION11201 Fully Automated Sample Preparation System GPC ULTRA; Final volume 5 mLThe combined GPC-Evaporator-System performs the completesample preparation process for the analysis of residues; also usableas a concentration unit; high-quality preparative LC-pump, vacuumchamber, stand-alone frequency controlled vacuum pump and vacuumcontrol; 100-110 VAC, 60 Hz. Column, rack, accessories are quotedseparately.NOTE: Racks, columns and accessories will vary by application and laboratory. Thisproduct sold only by quotation.75PRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / GPC PRODUCT LINEUtilizing the easy-to-use Vario PC software or the keypad,the system can customize evaporation proles for eachindividual sample and be setup for unattended operationovernight or through weekends. Matched with several autosamplerrack congurations, the EVA offers the exibilityand precision required in today’s busy laboratories.FEATURE HIGHLIGHTS• Reliable – Durable – Quiet• Sample Carry-Over Eliminated• Unattended Operation• Custom Evaporation ProfileEVAAUTOMATED EVAPORATOR FOR SERIAL CONCENTRATORSAt the heart of the EVA is a uniquely designed evaporationchamber that uses laser controls for accuracy and reproducibility.Utilizing the best features of rotary vacuum evaporationdesign, this chamber has been optimized for nalvolume accuracy and variable evaporation speeds.The rotary evaporator design uses centrifugal force andfrictional force between the wall of the rotating ask and theliquid sample to form a thin lm of warm solvent spreadover a large surface area. The forces created by the rotationsuppress violent, unpredicted boiling or bumping. Therotational design combines a silicon oil bath for consistentheat transfer with a laser-directed volume control to allowfor precision in either fast or gentle evaporation of solvents.The shape of the evaporation chamber has a unique, wideupper chamber and a narrow, tube-shape lower chamber.The width of the upper chamber allows solvent tospread around the walls, which optimizes heat transfer andmaximizes surface area for evaporation. The narrow lowerchamber acts as a collection tube and enables very accurate,level measurements with a laser at the end stages ofevaporation.POPULAR APPLICATIONS INCLUDE• Pesticides and Polychlorinated Biphenyls (PCB)– EN 1528, EPA 8082a• Pesticide Residues – EN 12393• Polycyclic Aromatic Hydrocarbon (PAH) – EPA 8310• Organochlorine Pesticide Residues – AOAC 984.21• Phthalates – EPA 8270c• …And Many More, Including USGS And USDA MethodsSYSTEM FEATURES• FLEXIBILITY IN PROCESSINGThe software allows 98 custom evaporation profiles to optimizefor each sample’s matrix requirements. Evaporation rate andrinsing cycle are variable, facilitating the alleviation of carry-over.Keypad or Vario Soft PC software controlled.• HIGH EVAPORATION EFFICIENCYEvaporation efficiency is determined by vacuum and heating.Vacuum is provided by a membrane pump with frequency controlfor virtually silent operation. Heating is accomplished by a closedsilicon oil bath for consistent and reproducible heating. Temperaturerange and precision from ambient to 70 °C +/- 1 °Cwith maximum evaporation rate of 10 mL/min.• DEFINED END VOLUMEFinal volumes of 1 mL or 2 mL/5 mL are possible withexcellent reproducibility.• SOLVENT EXCHANGEWhen solvent exchange is required, the EVA is capable of evaporatingto dryness and subsequently dissolving with a different solvent.Solvent exchange with vacuum control is also a possibility• HIGH RECOVERY RATES AND EXCELLENTREPRODUCIBILITYAutomation of the evaporation processes guarantees excellentreproducibility and high recovery rates.

LCTECH / GPC PRODUCT LINE• NO HOOD REQUIRED FOR OPERATIONSThe sample containers and autosampler vials are sealed withsepta and the condensed distillate is automatically discharged intoa collection vessel, alleviating the need for a fume hood.• FLEXIBLE SAMPLE RACK CONFIGURATIONSAvailable rack confi gurations include: 52 samples of 60mL each, 26 samples of 110 mL each, or 12 samples of250 mL each. The defined final volume of 1 mL autosamplervials or 16 mL vials are available. A cooling rack feature isoffered for sample cooling requirements.TECHNICAL DATA FOR THE EVA AUTOMATED EVAPORATION SYSTEMAmbient Temperature (Operational) 10 — 40 °CRelative Humidity (Operational) 0 — 95 %ELECTRONIC DATAPower Supply 230 V ± 10 % or 120 V ± 10 %Communication InterfaceRS 232 for Terminal and PC controlModem for remote serviceVACUUM UNITMax. Suction Performance0.8 M 3 / hPressure RangeAtmosphere — 2 mbarMEASURES & WEIGHTMeasures (W X D X H)750 X 600 X 1000 mmWeight (Operational)Ca. 110 kgCONCENTRATORTemperature Range Ambient – 70 ºCMax Evaporation Rate10 mL/min77EVA III EVAPORATION SAMPLE PREP INSTRUMENT CATALOG INFORMATIONCATALOG NO.DESCRIPTION11161 EVA, Sample Preparation System - Final Volume 1 mL,100-110 V / 50-60 Hz11172 EVA, Sample Preparation System - Final Volume 5 mL,100-110 V / 50-60 HzNOTES: Sample racks and accessories required for operation will vary by method.This product sold by quotation.PRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / GPC PRODUCT LINE• Can be integrated into LIMS systems and conforms to requirementsfor non-consecutive sample identification to processinglists (consecutive non-manipulability sample-ID).• Cleansing of the system is performed after each sample. Theentire flow path is rinsed including the sample needle therebyavoiding carry-over between samples.FEATURE HIGHLIGHTS• Reproducible High Recovery Rates• No Carry-Over• Easy Processing Of Complex Matrices• Reliable - Durable – Quiet• Unattended Operation• Keypad Or PC Control• Designed For Fatty Matrices• Sealed Sample VialsGPC VARIOAUTOMATED GEL PERMEATION CHROMATOGRAPHY SYSTEMGel permeation chromatography is a well known technologyfor the removal of fats, proteins, and other large biomoleculesfrom samples in order to prevent interference inthe analysis of chlorinated & organo-phosphorous pesticides,PCBs, polyaromatic hydrocarbons (PAH), and othersemi-volatile compounds.The GPC VARIO automates Gel Permeation Chromatography(GPC) technology for the clean-up of complex animaltissues, food products, environmental and many other typesof samples. The high recoveries are easily analyzed by GC-ECD, GC-MS, LC-MS, etc.Ideally suited for high-throughput laboratories requiringreliability and reproducibility, the GPC VARIO is optimizedfor unattended operation, low maintenance, and consistentrecoveries. Each component is engineered for robustnessand low maintenance, and the GPC VARIO provides theease of use necessary for increased throughput and worryfreeoperations.For 24 x 7 operation, the GPC VARIO is designed for limitedmaintenance and service. All ow paths are solvent resistant.Materials are glass, PTFE, stainless steel and PPS-E2.• Sample vials and receptacles for fractions can be sealed withsepta (not required) to prevent sample or solvent vapors fromescaping.POPULAR APPLICATIONS INCLUDE• Pesticides And Polychlorinated Biphenyls (PCB)– EN 1528, EPA 8082a• Pesticide Residues – EN 12393, DFG S19• Polycyclic Aromatic Hydrocarbon (PAH) – EPA 8310• Organochlorine Pesticide Residues – AOAC 984.21• Phthalates – EPA 8270c• Gel Permeation Clean-up – EPA 3640a• …And Many More, Including USGS And USDA MethodsSYSTEM COMPONENTS• LCTECH GPC COLUMNThe specially designed GPC column is DIN-compliant filled withS-X3 Bio-Beads, 200-400 mesh. The column construction isglass and PTFE, 1/8” PTFE inlet and outlet, rigid PTFE base andadjustable plunger, sturdy construction, and overpressure stable.• KEYPAD CONTROL WITH LCD DISPLAYA four line display with keypad input enables the system to operatein a stand alone mode. The menu driven input is self-explanatoryand intuitive for system operation.• SAMPLE HANDLING SYSTEMA precise swivel robot arm, in combination with a spring mountedneedle loop and an inert 10 mL syringe, transfers the liquid withinthe system. There are four solvent paths possible that allow forapplication flexibility and efficient system rinsing functions.• MULTIPLE INJECTION OPTIONSThe user can choose between sample loop overfill, or partial loopfill. Flexibility in sample handling allow for a variety of options,including multiple injections from the same vial with collection in asingle vial (ideal for fatty samples).

LCTECH / GPC PRODUCT LINE• SAMPLE LOOPThe supplied standard loop is made of PTFE and is set to 5.0 mL(preparative system) or 1 mL (analytical system). A measuringprotocol is supplied and other volumes are available on request.• SAMPLE RACKSRacks are available for flexible sample and GC vial combinations.Options include: 52 positions with 60 mL, 26 positions with 110mL, or 12 positions with 250 mL.• GPC PUMPThe system contains a robust PLC-controlled double pistonpreparative (analytical pump also available) pump adjustable from0.04 – 40.00 mL/min. Preparative pump technology has knownfor longevity and reliability in continuous operation environments.The pump flow rate is adjustable with pulsation less the 1.0 %.Over and low pressure shut-off is integrated, and with inert wettedcomponents, the reliability and low maintenance are superior toHPLC pump implementations.• SOFTWARE VARIOSOFT (OPTIONAL)The LCTech PC Windows VarioSoft can be integrated into LIMSsystems and conforms to requirements for non-consecutivesample identification to processing lists (consecutive non-manipulabilitysample-ID). The graphical user interface is modeled aftermost HPLC and GC software for familiar and intuitive control ofthe operations. Sample ID and Job List Sequence are encryptedand stored to meet all requirements for data security.• FIXED WAVELENGTH DETECTOR (OPTIONAL)The UV detector is a 254 nm fixed wavelength detector controlledby the VarioSoft GPC VARIO software to monitor the quality ofthe GPC columns.TECHNICAL DATA OF THE GPC VARIO SAMPLE CLEAN-UP SYSTEMAmbient Temperature (Operational) 10 — 40 °CRelative Humidity (Operational) 0 — 95 %ELECTRONIC DATAPower Supply 230 V ± 10 % or 120 V ± 10 %Communication InterfaceRS 232 for Terminal and PC control. Modemfor remote serviceGPC PUMPTypeDual PistonFlow RatesAnalytical: 0.10 – 9.95 mL/min Preparative:0.40 – 40.00 mL/minPressure40 bar (instrument)MEASURES & WEIGHTMeasures (W X D X H)Weight (Operational)CATALOG NO.750 X 600 X 1000 mmCa. 110 kgGPC VARIO CATALOG INFORMATIONDESCRIPTIONGel Permeation Chromatography (GPC) System - type VARIO,The basic unit comes with a preparative HPLC-pump (flow rate 0.4011006 to 40.0 mL/min); one sample loop 5 mL; 110 VAC, 50 Hz.Chromatography column, Sample Rack, bottles for equipping the rackas well as waste bottles need to be ordered separately.Gel Permeation Chromatography (GPC-system) - type VARIO-A,basic unit comes with an analytical HPLC-pump (flow rate 0.10 to11007 9.95 mL/min); one sample loop 1 mL; 110 VAC, 50 Hz.Chromatography column, Sample Rack, bottles for equipping the rackas well as waste bottles need to be ordered separately.NOTE: Racks, columns and accessories will vary by application and laboratory. Thisproduct sold only by quotation.79PRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / GPC PRODUCT LINEFEATURE HIGHLIGHTS• 4 Columns For Parallel Processing Or Individual Processing• Touchscreen Operation• Run-Time Fail-Safe Feature – Saves Solvents If Interrupted• Waste Level Sensors• Reproducible High Recoveries• Small FootprintGPC QUATTROPARALLEL PROCESSING FOR GPC SAMPLE CLEAN-UPGel permeation chromatography is a well-known technologyfor the removal of fats, proteins, and other large biomoleculesfrom samples in order to prevent interference inthe analysis of chlorinated & organo-phosphorous pesticides,PCBs, polyaromatic hydrocarbons (PAH), and othersemi-volatile compounds.The GPC Quattro Gel Permeation Chromatography (GPC)technology is well suited to the clean-up of complex animaltissues, food products, and environmental samples. Mainfractions can either undergo further clean-up or concentrationprior to analysis using GC-ECD, GC-MS, HPLC-MS, etc.Ideally suited for high-throughput laboratories requiringreliability and reproducibility, the GPC Quattro is designedfor 4 times the productivity of a single column system.Engineered for robustness and low maintenance, the GPCQuattro provides the ease of use necessary for increasedthroughput and worry-free operations.Utilizing the easy-to-use touch screen, the system can customizeelution proles for each individual sampleand column.OPERATION PARAMETERS• 4 Manual Injection Valves• Standard Is Four 5.0 mL Sample Loops; Others On Request• Forerun: 0 – 999 min• Main Run: 0 – 999 min• Tailings: 0 – 999 min• Global Minimum Pressure in bar or psi• Maximum Pressure for Each IndividualColumn in bar or psi• Methods/Names Assigned To Each ColumnCOLOR DISPLAY• Touchscreen With Color And Graphic SupportAnd Function Keys• Simulated Alphanumeric Input Via Touchscreen• Integrated Method Management With Input OfNew Methods And Names• System Activity Via Symbols• Color Coded Status Of Forerun, Main Run AndTailings For Each Individual Column• Actual Flow Path And Pressure Highlighted In Red• Time Bar Showing Elapsed Run Time• Remaining Run Time Via Figures• Actual And Set Maximum Pressure For Each Column• Errors By Symbol (Language Independent)In The Screen Of The Process Engineering• Chronological List Of Errors In Clear Text By Pressing F2

LCTECH / GPC PRODUCT LINESAFETY FEATURES AND ERROR MANAGEMENT• High And Low Pressure Shut-Off• Waste Level Sensor With Shut-Off• Defined “Load” And “Inject” Position With Safety Timer(Calculates minimum time from flow rate and sample loop size forre-switching to “load” position)• External Columns And Liquid Strictly SeparatedFrom Electric Compartment• Easy Access To Pump Housing• Password Protected System Setup• In Case Of Power Failure Timer, Values AreStored ‡ Resume Or Stop Process• Reset Of Timers When Run Was AbortedUPGRADES• Easy Upgrading Of General Software By CF Card• Upgrade 1: Individual Programming Of Each Column• Upgrade 2: Four Waste Level Sensors• Upgrade 3: Data And Process VisualizationOn PC Via VNC ViewerPAHPCB 12 3GPCQuattroPhthalatesDioxinParallel processing in four separately programmablecolumns improves productivity by 400 % at a fractionof the cost of four 1-column systems.4TECHNICAL DATA OF THE GPC QUATTROAmbient Temperature 10-40 ºCRelative Humidity (Operative) 0-95 %ELECTRONIC DATAPower Supply88-245 V Wide Range Power SupplyCommunication Interface Touchscreen, Optional PC VisualizationGPC PUMPSTypeDual PistonFlow Rates1-40 mL/minPressure62 bar (Instrument)MEASURES & WEIGHTSMeasures (W x D x H) 65 x 45 x 48 cm; Without ColumnsWeight (Operational) 20 kg (Operational)81CATALOG NO.GPC QUATTRO CATALOG INFORMATIONDESCRIPTIONGPC quattro Standard version, 96 - 240 V AC, 50-60 Hz11389(without columns)NOTE: Columns and accessories required for operation will vary by method. Thisproduct sold only by quotation.PRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / GPC PRODUCT LINEFEATURE HIGHLIGHTS• One To Four Column Throughput• Parallel Or Individual Processing With Multiple Columns• Run-Time Fail-Safe Feature-Saves Programming If Interrupted• Reproducible High Recoveries• Small FootprintGPC UNOEXPANDABLE GPC SAMPLE CLEAN-UPGel permeation chromatography is a well-known technologyfor the removal of fats, proteins, and other large biomoleculesfrom samples in order to prevent interference inthe analysis of chlorinated & organo-phosphorous pesticides,PCBs, polyaromatic hydrocarbons (PAH), and othersemi-volatile compounds.Control your budget and the quality of your results with theGPC Uno. Utilizing the GPC Quattro model features the GPCUno can be congured for single GPC operation or upgradedin the future to 4 column parallel processing. Engineered forrobustness and low maintenance, the GPC Uno provides theease of use and the expandability to grow with your laboratoryneeds.OPERATION PARAMETERS• 4 Manual Injection Valves• Standard 5.0 mL Sample Loop; Others On Request• Forerun: 0 – 999 min• Main Run: 0 – 999 min• Tailings: 0 – 999 min• Global Minimum Pressure in bar or psi• Maximum Pressure for Each Column in bar or psi• Methods/Names Assigned To Each ColumnCOLOR DISPLAY• Touchscreen With Color And Graphic SupportAnd Function Keys• Simulated Alphanumeric Input Via Touchscreen• Integrated Method Management With Input OfNew Methods And Names• System Activity Via Symbols• Color Coded Status Of Forerun, Main Run AndTailings For Each Individual Column• Actual Flow Path And Pressure Highlighted In Red• Time Bar Showing Elapsed Run Time• Remaining Run Time Via Figures• Actual And Set Maximum Pressure For Each Column• Errors By Symbol (Language Independent)In The Screen Of The Process Engineering• Chronological List Of Errors In Clear Text By Pressing F2

LCTECH / GPC PRODUCT LINESAFETY FEATURES AND ERROR MANAGEMENT• High And Low Pressure Shut-Off• Waste Level Sensor With Shut-Off• Defined “Load” And “Inject” Position With Safety Timer(Calculates minimum time from flow rate and sample loopsize for re-switching to “load” position)• External Columns And Liquid Strictly SeparatedFrom Electric Compartment• Easy Access To Pump Housing• Password Protected System Setup• In Case Of Power Failure Timer ValuesAre Stored• Reset Of Timers When Run Was AbortedUPGRADES• Easy Upgrading Of General Software By CF Card• Upgrade 1: Individual Programming Of Each Column• Upgrade 2: Four Waste Level Sensors• Upgrade 3: Data And Process VisualizationOn PC Via VNC Viewer1 21 1 23 41 2 3 1 2 3 4Expand throughput to your needsTECHNICAL DATA OF THE GPC QUATTROAmbient Temperature 10-40 ºCRelative Humidity (Operative) 0-95 %ELECTRONIC DATAPower Supply88-245 V Wide Range Power SupplyCommunication Interface Touchscreen, Optional PC VisualizationGPC PUMPSTypeDual PistonFlow Rates1-40 mL/minPressure62 bar (Instrument)MEASURES & WEIGHTSMeasures (W x D x H) 65 x 45 x 48 cm; Without ColumnsWeight (Operational) 20 kg (Operational)83CATALOG NO.GPC UNO CATALOG INFORMATIONDESCRIPTIONGPC Uno (with one GPC-line), 96 - 240 V AC, 50-60 Hz11601(without columns)Upgrade of GPC uno by one GPC-line, consists of pump,11607electronic devices & tubingNOTE: Columns and accessories required for operation will vary by method. Thisproduct sold only by quotation.PRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / GPC PRODUCT LINE3Intensity5000025000124GEL PERMEATION COLUMNSGPC COLUMNS; FUNCTIONALITY THROUGH AND THROUGHThe LCTech GPC columns are designed for easy and selfexplanatoryhandling. Due to their robustness, they can be usedfor every-day analysis in laboratories.The columns are available lled or unlled as well as indifferent lengths and lling heights. Suitable lling kits areprovided for easy relling.A patented xture is used for simple and clean relling ofBio-Beads for re-use.FEATURE HIGHLIGHTS• Leak Tightness Due To Sophisticated Construction With Only AFew Sealing Elements Only• Simple And Self-Explanatory Construction• Robust Layout• Pressure Stable (Max 20 Bars)• No Cross-Contamination In Subsequent Runs EvenWith Demanding Samples• Chemically Inert• Exchangeable 10 mm PTFE Frits• Available Ready-To-Use & Easily Refillable• DFG S19-Compliance (D25)• Column Cleaning & Refill Can Be Done By Customer00 5 10 15 20 25 30 35 40 45 50minMATERIALSFILTER FRITSSCREWEDFITTINGSPRESSURELCTech GPC Columns - Tested QualityQA CHROMATOGRAM OF A COLUMN GPC10011Ethylacetate/Cyclohexane 5.0 mL/minInjection Volume 5 mLUV Detection at 254 nm1. Corn Oil 25 g/L2. Di (2-Ethylhexyl) Phthalate 1 g/L3. Methoxychlor 0.2 g/L4. Perylene 0.02 g/LTo each fi lled LCTech GPC Column, a QA chromatogramis attached, showing the perfect separation of the testcomponents.GEL PERMEATION COLUMNS SPECIFICATIONSGlass, PTFE, aluminum and acetal, solvent resistance ofall parts10 µm, PTFE, exchangeableUNF 1/4” - 28 (standard lab screw connection)Max 20 barsP/NDIMENSIONS(TL x OD x ID)*FILLED GPC COLUMNSBIO-BEADSS-X3 FILLINGBEDLENGTHSOLVENT** METHOD (e.g.)GPC10011 500 x 40 mm, 25 mm 50 g 320 mm EA/CY 1/1 DFG S19GPC30011 660 x 40 mm, 25 mm 70 g 450 mm EA/CY 1/1 Modified USEPA 3640 AGPC40011 425 x 40 mm, 20 mm 24 g 225 mm EA/CP 7/3 FSIS CLG-CHC3GPC50011 780 x 55 mm, 25 mm 70 g 540 mm DCUSEPA 3640 A,EPA Method 8270 C, 8141 BGPC90011 500 x 40 mm, 25 mm 35 g 200 mm DC/HX 1/1 USP Monographs: Lanolin* TL = Total Length, OD = Outer Diameter, ID = Inner Diameter** CY = Cyclohexane; CP = Cyclopentane; DC = Dichlorometane; EA = Ethyl Acetate; HX = Hexane,Mixing ratio on volume basisNOTE: To ensure we sell you the best possible column for your needs,please contact us with any questions, or for a quotation.

LCTECH / GPC PRODUCT LINEGPC QUATTROCATALOG NO.DESCRIPTION11389GPC quattro, Standard version, 96 - 240 V AC, 50-60 Hz(without columns)11391 Software UPGRADE (indiviual programming of each channel/column)11392 Waste Level Sensor UPGRADE (three more waste sensors)11393 Data & Process Visualization on PC via VNCViewerGPC UNOCATALOG NO.1160111607DESCRIPTIONGPC Uno (with one GPC-line), 96 - 240 V AC, 50-60 Hz(without columns)Upgrade of GPC uno by one GPC-line, consists of pump,electronic devices & tubingGPC QUATTRO & UNO ACCESSORIESCATALOG NO.DESCRIPTION10071 Syringe, 1.0 mL (Needle has to be ordered addionally)10072 Syringe, 5.0 mL (Needle has to be ordered separately)10074 Syringe, 10.0 mL (Needle has to be ordered separately)10075 Needle/Probe for syringesGPC VARIO85CATALOG NO.DESCRIPTIONGel Permeation Chromatography (GPC) System - type VARIO,11006 The basic includes preparative HPLC-pump; one sample loop 5 mL; 110 VAC, 50 Hz.Chromatography column, Sample Rack, bottles for equipping the rack as well as waste bottles are quoted separately.Gel Permeation Chromatography (GPC-system) - type VARIO-A,11007 basic unit includes analytical HPLC-pump; one sample loop 1 mL; 110 VAC, 50 Hz.Chromatography column, Sample Rack, bottles for equipping the rack as well as waste bottles are quoted separately.10835 Upgrade GPC VARIO Upgrade-set for an additional column, valves, tubes. This enables sample separation on different columns depending on the matrix.10879 Upgrade GPC Vario for High-Pressure VersionVarioSoft - PC-Software to control GPC Vario.10273PC-Requirements: Windows 98SE or higher; 2 GB Hard Drive; one RS232 Interface (Com-Port); CD Rom. Computer sold separately.GPC ULTRACATALOG NO.DESCRIPTIONFully Automated Sample Preparation System GPC ULTRA; Final volume 5 mL11201The combined GPC-Evaporator-System performs the complete sample preparation process for the analysis of residues; also usable as a concentration unit;high-quality preparative LC-pump, vacuum chamber, stand-alone frequency controlled vacuum pump and vacuum control; 100-110 VAC, 60 Hz. Column, rack, accessoriesare quoted separately.10880 Fully Automated Sample Preparation System GPC ULTRA; Final volume 5 mL as above, but with high-quality analytical LC-pump10881 Special system for PAH in Oils: Fully Automated Sample Preparation System GPC ULTRA; preparative LC-pump10836 Online-Combi-Method: EVAporation-GPC-EVAporation covers methods from dried & filtered raw extract to GC-Vial10778 Cooling unit for LCTech systems with turntable rack; recommended for all applications with temperature sensitive samples or solvents with low boiling points10518VarioSoft 1.0 - PC-Software to run GPC ULTRAPC-Requirements: Windows 98SE or higher; 2 GB Hard Drive; one RS232 Interface (Com-Port), CD-ROM. Computer sold separately.PRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / GPC PRODUCT LINEEVA IIICONSUMABLESCATALOG NO.DESCRIPTION11002 Final Volume 1 mL, 240 VAC, 60 HZ11161 Final Volume 1 mL, 100-110 VAC / 50-60 HZ11003 Final Volume 5 mL, 240 VAC, 60 HZ11172 Final Volume 5 mL, 100-110 VAC / 50-60 HZPC-Software to control EVAPC-Requirements: Windows 98SE or higher; 2 GPC Hard Drive;10274one RS232 Interface (Com-Port); CD Rom Drive. Computer soldseparately.Cooling unit for LCTech systems with turntable rack;10778 recommended for all applications with temperature sensitive samplesor solvents with low boiling points.GPC ACCESSORIESCATALOG NO.DESCRIPTIONP-250-16Rack for 12 samplessample presentation in 16 mL /fractions in 250 mLP-100-16Rack for 26 samplessample presentation in 16 mL /fractions in 110 mLP-60-16Rack for 52 samplessample presentation in 16 mL / fractions 60 mLRack for 12 samplesP-100S-16 sample presentation in 16 mL / fractions in pear shaped bottomflasks, 100 mL & 50 mL; grinding: NS 14/23for 8 samplesP-250R-16 sample presentation in 16 mL / fractions in standard flasks, roundbottom 250 mL (grinding: NS 29/32)11768 Stopper NS 14/23, PP with hole for needleV0250KL Stopper NS 29/32, PP with hole for needle10430Adapter / Reduction insert; suitable for all racks P/N P-...-16,for Vials 5 mL, P/N V0005 / round bottomF10000-DVReservoir bottle glass, 10 L, GL 45; Including sealing cap;1/8”-connectionF10000-DReservoir bottle glass, 10 L GL 45 including sealing cap for directconnection to the waste line11170Chiller/Circulating Cooler; operational range - 20 °C to 40 °C;for GPC ULTRA & EVA; incl. tubing & thermo fluidGPC COLUMNSCATALOG NO.GPC10011GPC20011GPC30011GPC40011GPC50011GPC90011DESCRIPTIONColumn GPC, 25 id x 500 mm, 50 gColumn GPC, 10 id x 500 mm, 10 gColumn GPC, 25 id x 660 mm, 70 gColumn GPC, 20 id x 425 mm, 24 gColumn GPC, 25 id x 780 mm, 70 gColumn GPC, 25 id x 500 mm, 35 gCATALOG NO.F060F100-DF100F250-DF250V0025-SLV0024-SLV0025-DV0016V0016-SLV0016-DV0016-SV0004V0004-SLV0004-SV0004-DV000510430V0001V0001-BBOTTLES (MAINLY USED FOR GPC & EVA)DESCRIPTIONScrew-thread bottle, 60 mL, with cap (GL24), sample rack P-60- ... ;with screw cap (V0024-SL) and w/o Seals (V0025-D)100/pkScrew-thread bottle,120 mL, with sealing cap (GL25), with aperture for seal suitable forsample rack P-100- ... ;2/pkScrew-thread bottle, with concave bottom for quantitative liquid transferNominal content max. 110 mL; with cap (GL25) for seal;For sample rack P-100...2/pkScrew-thread bottle,250 mL, cap (GL25) with aperture for seal; For sample rack P-250- ... ;2/pkScrew-thread bottle, concave bottom for quantitative liquid transfer250 mL, with cap (GL25) with aperture for seal;For sample rack P-250- ... ;2/pkCAPS & SEALS FOR A.M. BOTTLESSealing cap with hole (for seal break-through) For screw-threadbottles F-100-D and F-250-D (GL25)10/pkSealing cap with hole (for seal break-through) For screw-threadbottles F-060 (GL24)100/pkSeals for all screw-thread bottles F-250-D, F100-D, F-060100/pkScrew-thread vials, 16 mL, flat bottom, open (no seal);For all racks P-...-16100/pkScrew cap with hole, black for 16 mL-vials (Part-No.: V-0016),100/pkSeal G18 for 16 mL-vials PTFE/silicone, (Part-No.: V-0016)100/pkScrew cap without hole, black for 16 mL-vials (Part-No.: V-0016),100/pkSAMPLE VIALSScrew-thread vials, clear, 4 mL, flat bottomDimensions: 45 x 14.75 x 0.95 mm100/pkScrew cap with hole, black for 4 mL-vials (Part-No. V-0004),100/pkScrew cap without hole, black for 4 mL-vials (Part-No.: V-0004),100/pkSeal G13 for 4 mL - vials PTFE/silicone, for 4 mL-vials (Part-No.: V-0004),100/pkSAMPLE VIALSRound bottom vials, 5 mL, with aperture for seal. For sample racksP... 16 ; For use only with adapter (reduction insert) P/N 10430w/o screw cap (V0004-SL) and sealing cap (V0004-D)125/pkAdapter / Reduction insert; suitable for all racks P/N P-...-16,for Vials 5 mL, P/N V0005 / round bottomGC SAMPLE VIALSInjection vials, clear glass, 1.5 mL for GC-systemsCrimp-top vials 11 x 4 mmDimensions 32 x 11.5 x 0.9 mm100/pkCrimp seals, R11, with inert seal washer PTFE/Silicone/PTFE, red100/pk

LCTECH / MYCOTOXIN PRODUCT LINEUVEPHOTOCHEMICAL REACTORUsed for Detection Enhancement for Aatoxins, Phenylurea Pesticides, Barbiturates and Other Compounds. Photochemicalderivatization is a simple, inexpensive and exible technique that improves sensitivity and selectivity of detection for abroad range of analytes. Among the applications for the photochemical reactor are analysis of Aatoxins in foods, PhenylureaPesticides in water and Barbiturates in biological samples. Photochemical derivatization also allows identication ofclosely related compounds such as polyphenols.Pickering Laboratories Multi-residue Mycotoxins method for DON, Aatoxins, Fumonisins, Ochratoxin A and Zearalenoneemploys photochemical derivatization for Aatoxins, allowing detection at sub-ppb levels.The photochemical reactor has a 254 nm lamp and a knitted reactor coil.87FEATURE HIGHLIGHTS• 254 nm UV Low Pressure Lamp With Cooled Reflector Tube• Long Term Stability Of Lamp And Coil• High Light Transmission• Safety Shut-off Turns Off Lamp If Cover Is Removed• Robust Steel Housing To Meet Laboratory Requirements• Special Designed Fluorocarbon Coil• Comparable To Electrochemical Derivatization With Cobra Cell(DG Joint Research Center Of The EuropeanCommission In The Institute For Health And Consumer Protection)• AOAC Accepted Methodology• Standard Reactor Volume Is 1.0 mLPRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / MYCOTOXIN PRODUCT LINEUV DERIVATIZATION RESULTS IN CLEAR PEAKS FOR AFLATOXINSNote the short run time: B1 elutes at 9.5 Min10,00010,00090009000B18000B28000G17000G2700060006000B2Intensity50004000Intensity50004000G230003000200020001000G1B11000000 1 2 3 4 5 6 7 8 9 10Without UVE: Low response for G1 and B1Min0 1 2 3 4 5 6 7 8 9 10MinWith UVE: High response and no band spreadingUVE SPECIFICATIONSUVE CE CERTIFIEDUV LAMPYes254 nmHPLCREACTOR COILDIMENSIONSSpecial14.5 x 8.5 x 27 cmFLDPOWER INPUTWEIGHT50 W3 kgEasy Handling: Simply place the UVEbetween your HPLC device and detector,switch it on - ready to use!UVE CATALOG INFORMATIONCATALOG NO.DESCRIPTION1100-3347 Photochemical Reactor 1.0 mL, 120 V1100-3348 Photochemical Reactor 1.0 mL, 240 V1552-0024 Lamp, 254 UV, Photochemical1100-3505 Reactor Coil, Photochemical 1.0 mL

LCTECH / MYCOTOXIN PRODUCT LINEACCECLEANAUTOMATED SAMPLE CLEAN-UP FOR IAC & SPEThree Needle Parallel Sample Clean-up for ImmunoafnityColumns. The LCTech AcceCLEAN processes up to 30samples in one batch, and runs three samples simultaneously.Sample clean-up of crude extracts for a broad rangeof matrices is performed using immunoafnity columns.Utilizing the easy-to-use the keypad, the system can customizeproles for each individual sample rack and setup forunattended operation overnight or through weekends. TheAcceCLEAN offers the exibility and precision requiredin today’s busy laboratories.FEATURE HIGHLIGHTSAFLATOXINS G2 G1 B2 B1Area Count Sample 865 1490 1108 2390Area Count Blank 0 0 0 0Comparison of recovered area counts of a 300 ppbsample with the immediately following blankLCTECHCOLUMN TYPEOtaCLEANMATRIXPeanutButterREC. RATEMANUAL (%)REC. RATE AUTOMATEDWITH ACCECLEAN (%)97 97OtaCLEAN Maize 94 9389• Three Parallel Operating NeedlesAfla-OtaCLEAN Rice 91 93• No Cross-Contamination Or Carry-Over From Sample-To-Sample• Eight-Port Valve Enables Seven Different Solvents In AdditionTo Air FlowComparison of recovery rates of Ochratoxin A with manualand automated operation of Immunoafnity columns withdifferent matrices• Rack Platform With Up To Ten Racks, 3 Samples Each• Different Method For Each Rack Available• Holds Up To 50 Stored Methods• Rugged Construction For Low Maintenance AndContinuous Operation• Easy-To-Use Keypad Operation• 1 mL, 3 mL, 6 mL / Columns• System Can Easily Handle Most SPE Columns• Compatible With Any Immunoaffinity Columns• Virtually Silent OperationRecovery Rate [%]10080604020G2 G1 B2 B1 G2 G1 B2 B1 G2 G1 B2 B1 G2 G1 B2 B1G2G1B2B1ACCECLEAN CATALOG INFORMATIONCATALOG NO.DESCRIPTION11020 AcceCLEAN robotic system for the automated handling of IAC columns11262 Rack 3 mL vials, three samples simultaneouslyNOTE: Additional racks can be found on page 96.0.02.5 50100200Total concentration of all aflatoxins in the matrix [ppb]Recovery rates of Aatoxins in peanut butter,using 3 mL AaCLEAN columns in AcceCLEANColumns and accessories required for operation will vary by method. This productsold only by quotation.PRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / MYCOTOXIN PRODUCT LINESAMPLE CLEAN-UP COLUMNFOR AFLATOXIN ANALYSISAflaCLEAN from LCTech was developed for the sampleclean-up of foods, grains, feeds, etc. for aflatoxin analysisusing HPLC post-column derivatization or other techniques.The AflaCLEAN column is selective for B1, B2, G1 and G2.The maximum loading capacity is 150 ng of Aflatoxin. Theshelf-life is 18 months (from date of manufacture) at roomtemperature.AFLACLEAN, OTACLEAN & AFLAOTACLEANCOMBINATION CLEAN-UP COLUMNIMMUNOAFFINITY SAMPLE CLEAN-UP COLUMNSAatoxins and Ochratoxin A are produced by fungi, e.g.Aspergillus and Penicillium species. Therefore both toxintypes are found together in many foods and animal feeds,e.g. cereals. Thus it is appropriate to analyze one extractfor the occurrence of both mycotoxins in a single step.Of signicant assistance is the clean-up of extracts by a combinedimmunoafnity column for both mycotoxin types inone step. The subsequent analysis may then be performed byHPLC with post-column derivatization or other techniques.SIMULTANEOUS CLEAN-UP OFAFLATOXINS AND OCHRATOXIN AThe combination sample clean-up column Aa-OtaCLEANfrom LCTech is very tolerant towards many matrices andallows for a comprehensive clean-up of the aatoxins B1,B2, G1 and G2 as well as Ochratoxin A. The high maximumcapacity of 150 ng for Aatoxin B1 and 200 ng forOchratoxin A provides a wide measurement range. Witha shelf-life of 1 year (from date of manufacture) at roomtemperature, the storage and use of the columns is veryconvenient.EXAMPLE RECOVERIES OF AFLATOXIN AND OCHRATOXINSMATRIX B1 B2 G1 G2 OTAMaize (Afla 10 ppb, OTA 14.3 ppb) 107 % 91 % 103 % 75 % 97 %Rice (Afla 10 ppb, OTA 14.3 ppb) 107 % 93 % 98 % 85 % 101 %Malt (Afla 10 ppb, OTA 14.3 ppb) 98 % 99 % 97 % 70 % 96 %Raisins (Afla 10 ppb, OTA 14.3 ppb) 99 % 106 % 101 % 69 % 97 %AFLATOXINS & OCHRATOXIN A SAMPLE CLEAN-UPCOLUMN CATALOG INFORMATIONCATALOG NO. DESCRIPTION QTY1705-0005 Alfa-OtaCLEAN sample clean-up column 25/pkgNOTE: Larger quantities available upon requestEXAMPLE RECOVERIES OF AFLATOXINMATRIX B1 B2 G1 G2Peanut Butter 95 % 98 % 93 % 84 %Peanuts 104 % 94 % 96 % 85 %Dried Distillers Grain 109 % 97 % 90 % 77 %Maize 101 % 98 % 103 % 80 %AFLATOXIN SAMPLE CLEAN-UP COLUMN CATALOG INFORMATIONCATALOG NO. DESCRIPTION QTY1705-0003 AlfaClean sample clean-up column, 3 mL 25/pkgNOTE: Larger quantities available upon request160125100755025mVEmission: 460 nm-200 1.3 2.5 3.8 5.0 6.3 7.5 8.8 10.0minDistillers grain spiked with 5 ppb total Aatoxins400300200100mV-500.0 2.5 5.0 7.5 10.0minG2G1Peanut Butter spiked with 5 ppb AatoxinB2B1

LCTECH / MYCOTOXIN PRODUCT LINESAMPLE CLEAN-UP COLUMN FOROCHRATOXIN ANALYSISOtaCLEAN was developed for sample clean-up of foods,grains, feeds, etc. for Ochratoxin A analysis using HPLC orother techniques. The antibody employed possesses a veryhigh Ochratoxin A specicity. This leads to exceptionalchromatographic results without any interfering secondarysignals and very high recovery rates. Independent of thecomplexity of the matrix, excellent results can be achieved.200150100mVOta AEmission: 440 nmThe maximum loading capacity is 200 ng Ochratoxin A.The shelf-life is 18 months (from date of manufacture) atroom temperature.50EXAMPLE RECOVERIES OF OCHRATOXIN AMATRIXRECOVERY RATEDried Distillers Grain 102 %Duran Wheat 92 %Coffee 100 %Red Wine 108 %Rye Bran 91 %Beer 96 %Grapes 91 %Horse Feed 94 %Wort 107 %OCHRATOXIN A SAMPLE CLEAN-UP COLUMN CATALOG INFORMATIONCATALOG NO. DESCRIPTION QTY1705-0004 OtaCLEAN sample clean-up column 25/pkgNOTE: Larger quantities available upon request-200 1.3 2.5 3.8 5.0 6.3 7.5 8.8 10.0min700500250mVDistillers grain spiked with5 ppb Ochratoxin AOta A9100.0 2.5 5.0 7.5 10.0minRoasted Coffee spiked with5 ppb Ochratoxin APRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / MYCOTOXIN PRODUCT LINEOTAREAD & AFLAREADAFLATOXIN AND OCHRATOXIN ELISA TEST KITSThe contamination of food and feed is a worldwide problem.Under inappropriate storage conditions fungi will producesecondary metabolites like mycotoxins, which often renderconsumption or further processing impossible. As a resultof these toxic and hazardous conditions, analyses must beextremely effective, sensitive and reliable to conform to thestrict regulations.The ELISA Test Kits from LCTech meet and exceed allregulatory requirements. The immunological test procedureis based on antibody-antigen recognition and enzymaticcolor reaction. It leads to a reliable result after a short incubationperiod and performs quick and sensitive screeningof a variety of food products like rice, corn, cocoa, coffee,peanut butter, beer, wine, liquorice and animal feed.LCTech ELISA kits are delivered ready-to-use and can directlybe used for screening sample extracts without further pretreatment.These kits consist of a shrink-wrapped, coatedELISA plate, the standard solutions, all necessary reagentsand a comprehensive manual to guide you through theeffective and easy analysis.FEATURE HIGHLIGHTS• Reduced Costs (No Analytical Instruments Needed)• Very High Sample Throughput (96 Samples incl. stds. in 130 min)• Unmatched Aflatoxin B1 Sensitivity (0.2 ppb)• Unmatched Ochratoxin A Sensitivity Of (0.6 ppb for Liquids)• Fast And Reliable Results• Highest Reproducibility• Long Shelf-Life (12 Months From Date Of Manufacture)• Easy Handling• Wide Range Of Matrices Including Dried Distillers Grain• Each Kit Contains 96-Well Plate, Standards, And AllNecessary SolutionsELISA KITS CATALOG INFORMATIONCATALOG NO.DESCRIPTION11139 AflaREAD, ELISA Kit (96 well) for the detection of Aflatoxin B111853AflaREADfast, ELISA Kit for determination of Aflatoxin in corn andpeanut based feed11068 OtaREAD, ELISA Kit (96 well) for the detection of Ochratoxin A11854OtaREADfast, ELISA Kit (96 well) for the detection of Ota A in corn,coffee, and peanut based feedOTAREAD OCHRATOXIN AThe LCTech ELISA kit test can be used with solid or liquidmatrices. The detection range for solid matrices is 2-20 ppb,for beer 0.6-6 ppb and for wine 0.8-8 ppb.Absorption ( % of Standard 1 )10090807060504030201000.100 0.200 0.400 0.600 0.800 1.0Ochratoxin A Concentration ( ng/mL )Representative test of a standard, with exemplarylimits in accordance with EU regulations.ALFAREAD AFLATOXIN AThe test range is 2-100 ppb.Absorption at 450 nm [%]10090807060504030201002 5 10 20 50 100Aflatoxin B1 Concentration in the Maxtrix [ppb]Representative test of a sample, with exemplarylimits in accordance with EU regulations.

LCTECH / MYCOTOXIN PRODUCT LINEAFLAREAD FASTThis easy-to-use kit is for the detection and quantication ofAatoxin B1 in corn and peanut based feed. The test durationis 40 min (including color development). The test rangeis 2 - 100 ppb.10090Absorption ( % of Standard 1 )807060504030201000.100 0.250 0.500 1.0 2.5 5.0Aflatoxin B1 Concentration ( ng/mL )AaREAD FastOTAREAD FASTOtaREAD fast is a competitive ELISA for the detection andquantication of Ochratoxin A in corn and peanut basedfeed or coffee. The test duration is 25 min (including colordevelopment) The test range 2 - 20 ppb. The antibody usedin this kit shows cross-reactivity against Ochratoxin B orOchratoxin alpha.9310090Absorption ( % of Standard 1 )807060504030201000.100 0.200 0.400 0.600 0.800 1.0Ochratoxin A Concentration ( ng/mL )OtaREAD FastPRODUCT CATALOGPICKERING LABORATORIES10/11

LCTECH / MYCOTOXIN PRODUCT LINEACCECLEAN SAMPLE CLEAN-UPACCECLEAN, SAMPLE PREPARATION SYSTEM FOR IAC & SPE COLUMNSCATALOG NO.DESCRIPTION11020 AcceCLEAN, robotic system, up to 30 samples in one sequenceSpecial Rack for 3 mL IAC-Cartridge, 3 samples parallel; for 30 mL11262sample vials (V0030) & 4 mL Collection Vial (V0004)Rack* for 1 mL SPE-Cartridge, for usage of 30 mL sample vials11630(V0030), 3 samples parallelRack* for 3 mL SPE-Cartridge, for usage of 30 mL sample vials11631(V0030), 3 samples parallelRack* for 6 mL SPE-Cartridge, for usage of 30 mL sample vials(V0030), 3 samples parallel11632* works only with an eluate rack, please see belowOne of the following eluate racks is needed for 11630-1163211633 ELUATE Rack for 4 mL vials (P/N V0004)11634 ELUATE Rack for 10 mL vials (P/N V0010)CONSUMABLES (VIALS & CAPS) FOR USAGE WITH ACCECLEAN10933 Inlet Cap for 3 mL SPE-columns, 100/bag11629 Inlet Cap for 6/8 mL SPE-columns, 100/bagVials with snap-on-lid, total volume 30 mLV0030 (Seals & snap-on-lid for storage only)Packing unit 200/pkVials with snap-on-lid, total volume 10 mLV0010 (Seals & snap-on-lid for storage only)Packing unit 100/pkUVE - UV-DERIVATIZERUVE - PHOTOCHEMICAL REACTOR FOR AFLATOXIN ANALYSISCATALOG NO.DESCRIPTION1100-3348 UVE - Photochemical Reactor for Aflatoxin Analysis;254 nm lamp; ready to use, CE-certification, 240 V, 50 Hz1100-3347 UVE - Photochemical Reactor for Aflatoxin Analysis;254 nm lamp; ready to use, CE-certification, 90-126 VAC, 50/60 Hz1100-3505 Reactor coil (only for UVE - reactor) ; 1.0 mL, other coil size on request1552-0024 UV replacement lampMINIPHOTOMETER FOR USAGE WITH ELISA-STRIPES(E.G. LCTECH ELISA KITS FOR MYCOTOXIN-ANALYSIS)11689 Photometer with 450 nm filter & power supply11720 3Ax6 Battery pack boxSAMPLE CLEAN-UP COLUMNSCATALOG NO.DESCRIPTION1705-0003 AflaCLEAN - 25 columns/box1705-0004 OtaCLEAN - 25 columns/box1705-0005 Afla-OtaCLEAN - 25 columns/box1705-0001 SPE Glyphosate clean-up - 50 columns/boxHISTAMINE ANALYSIS KITSHistamine is produced by microbial decomposition of theamino acid histidine and is stored in the cells.In perishable foodstuff like sh, sausages, cheese, wine ormilk the concentration of histamine is an indicator for thefreshness and ultimately for quality of product.The consumption of histamine-containing food can lead toserious poisoning. This is why it is absolutely necessary tohave quick and reliable controls for the histamine concentrationin both food and feed.From an in-situ quick test to a complete kit for the analysisof biogenic amines, LCTech and Pickering offer reliableand inexpensive tools for the analysis of histamine.CATALOG NO.DESCRIPTION11755 HistaQUICK Fish Meal, 48 samples11754 HistaREAD ELISA Kit, 96 samplesELUFIXCOLUMNS FOR THE H53 METHODAND OTHER FLORISIL® APPLICATIONSThe determination of the hydrocarbon oil index in surface,industrial and process water in compliance with EN ISO9377-2 (H53) is daily routine in many laboratories. Usingintelligent sample preparation can reduce time and costs forthe analysis signicantly.The LCTech Elux columns can be used for all kinds ofsamples. They are delivered factory sealed and can beinstantly used without further pre-treatment. The single-usecolumns are lled with 2g, 4g or 10g of activated Florisiland 2g anhydrous sodium sulphate. The use of inert materialslike glass, ber glass and PTFE excludes interactionswith the analytes as well as contaminations.CATALOG NO.937093719372DESCRIPTIONELUFIX Ready-to-use columns; 2 g FLORISIL® (150-250 µm) & 2 ganhydrous sodium sulfateELUFIX Ready-to-use columns; 4 g FLORISIL® (150-250 µm) & 2 ganhydrous sodium sulfateELUFIX Ready-to-use columns; 10 g FLORISIL® (150-250 µm) & 2g anhydrous sodium sulphateELISA KITSCATALOG NO.DESCRIPTION11139 AflaREAD, ELISA Kit (96 well) for the detection of Aflatoxin B111853AflaREADfast, ELISA Kit (96 well) for determination of Aflatoxin incorn and peanut based feed11068 OtaREAD, ELISA Kit (96 well) for the detection of Ochratoxin A11854OtaREADfast, ELISA Kit (96 well) for the detection of Ota A in corn,coffee, and peanut based feed

LCTECH / MYCOTOXIN PRODUCT LINEELUVACVACUUM MANIFOLD FOR SOLID PHASE EXTRACTIONThe EluVac vacuum manifold allows simultaneous processingup to 20 samples under vacuum. It is the ideal solutionfor enriching, concentrating and isolating analytes using solidphase extraction.It is mechanically and chemically robust, exible and can optimallybe used for a wide range of matrices in environmental,food and feed, pharmaceutical and clinical applications.A special highlight: By simply turning the head, you canswitch from the “waste” to the “collect” position withoutdisassembling the device.Racks for ten 60 mL and twenty 4 mL as well as for six 100 mLround bottom bottles (NS 29/32) are available for the manifold.For waste, either a 600 mL beaker glass is placed directly inthe system or a central tube leads the waste into an externalcontainer. Thus the EluVac device can also be used for largevolume samples e.g. water or milk.ELUVAC CATALOG INFORMATIONCATALOG NO.DESCRIPTIONEluVac, Vacuum Manifold Package / 20 samples simultaneously,11146 consists of: EluVac - Vacuum Manifold (p/n 11048), sample rack(p/n 11098) and collection rack for 60 mL bottles (p/n 11118)1114711739EluVac, Vacuum Manifold Package / 20 samples simultaneously,consists of: EluVac - Vacuum Manifold (p/n 11048), Probenrack(p/n 11560) collection rack for round bottom bottles for rotaryevaporator (p/n 11112) and 6 pear shaped rotary evaporator bottles(p/n 11106)EluVac, Vacuum Manifold Package / 20 samples simultaneously,- for high sample volume, e.g. water, milk, etc.consists of: EluVac - Vacuum Manifold (p/n 11048), collectionrack for 4 mL-vials (p/n 11105) and 10 SPE-column adapters (p/n11734) and Woulff’s bottle, 10 L (p/n F10000B)11048 EluVac, Vacuum Manifold / 20 samples simultaneously110981111811105Sample Rack for up to 10 sample reservoirs (p/ns 10896 or 9370-1/ see below) - on topCollection rack for 60 mL bottles (P/N F060) / 10 samplessimultaneouslyCollection rack for 4 mL Vials (P/N V0004) / 20 samples simultaneouslyfor usage of max 6 pear shaped rotary evaporator flasks:11560 Sample Rack for up to 6 sample reservoirs - on top11112111461114711739Collection rack for 100 mL pear shaped rotary evaporator bottles, NS29/32 (P/N 11106)EluVac, Vacuum Manifold Package / 20 samples simultaneously,consists of: EluVac - Vacuum Manifold (p/n 11048), sample rack(p/n 11098) andcollection rack for 60 mL bottles (p/n 11118)EluVac, Vacuum Manifold Package / 20 samples simultaneously,consists of: EluVac - Vacuum Manifold (11048), Probenrack (p/n11560)collection rack for round bottem bottles for rotary evaporator (p/n11112) &6 pear shaped rotary evaporator bottles (p/n 11106)EluVac, Vacuum Manifold Package / 20 samples simultaneously, -for high sample volume , e.g. water, milk etc.consists of: EluVac - Vacuum Manifold (p/n 11048),collection rack for 4 mL-vials (p/n 11105) & 10 SPE-column-adapters(p/n 11734) & Woulff’s bottle, 10 L (p/n F10000B)ELUVAC CATALOG INFORMATIONCATALOG NO.DESCRIPTION11125ME 2C, diaphragm pump, one stage, “Chemistry” RECOMMENDED!230 V, 50-60 Hz, cable, plug & instructions111261112311124ME 2C, diaphragm pump, one stage, “Chemistry” (USA, Korea, Japan)100-120/200-230V / 50-60 Hz, cable, plug & instructionsME 2, diaphragm pump, one stage, “Aluminium”230 V, 50-60 Hz, cable, plug & instructionsME 2, diaphragm pump, one stage, “Aluminium” (USA, Korea, Japan)100-120/200-230V / 50-60 Hz, cable, plug & instructions11043 Adapter ELUFIX-Column to Luer (10 pcs./pck) 5 packages and more117349370-1Adapter for SPE-columns (inlet side);connects any size column onto a 1, 3, 6 or 15 mL column, with 1.5 maspiration tubing when ordering 10 units or moreReservoir (2 pcs/Pck), made of glass; for sample load (120 mL);labwasher-proof; reusable, includes screwing and PTFE sealSeal fits to LCTech glas columns (p/ns 9370, 9371, 9372) whenordering 4 packages and more9373-2 Seals for sample reservoir P/N 9371-1, PTFE, 10 pcs/pck10896Reservoir (2 pcs/Pck), made of glass; for sample load (120 mL);labwasher-proof; reusable, includes screwing and PTFE sealSeal fits to immunoaffinity columns when ordering 4 packages and more10909 Seals for sample reservoir P/N 10896, PTFE, 10 pcs/pck10081F10000BClamp, 3 fingers with rubber (for usage with reservoir) 5 packagesand moreBottle, coated, 10 L, with special screw cap and 2 m tubingMINERAL OIL HYDROCARBON INDEX(H53-METHOD) ELUFIX COLUMNSACCECLEAN, SAMPLE PREPARATION SYSTEM FOR IAC- & SPE COLUMNSCATALOG NO.DESCRIPTION9370 ELUFIX Ready-to-use columns; made of glass for method H53; lotcertification enclosed; suitability check and result performance incompliance with EN ISO 9377-2; Filling material: 2 g FLORISIL®(150 - 250 µm) & 2 g anhydrous sodium sulfate welded in strips offive; 10 strips per package4 packages and more (200 columns)8 packages and more (400 columns)16 packages and more (800 columns)9371 ELUFIX Ready-to-use columns; made of glass for method H53;lot certificate enclosed; suitability check and result performance incompliance with EN ISO 9377-2Filling material: 4 g FLORISIL® (150 - 250 µm) & 2 g anhydroussodium sulfate welded in strips of five; 10 strips per package4 packages and more (200 columns)8 packages and more (400 columns)16 packages and more (800 columns)9372 ELUFIX Ready-to-use columns; made of glass for method H53;lot certificate enclosed; suitability check and result performance incompliance with EN ISO 9377-2Filling material: 10 g FLORISIL® (150 - 250 µm) & 2 g anhydroussodium sulfate welded in strips of three; 10 strips per package4 packages and more (120 columns)16 packages and more (460 columns)32 packages and more (960 columns)10374 Empty Glass Column, Fiolax, 105 mm length x 17 mm diam.;100 pcs/pck4 packages and more11156 Empty Glass Column, Fiolax, 180 mm length x 17 mm diam.;100 pcs/pck4 packages and more10230 Glass Fiber Filters, Diameter 15 mm (100 pcs/box)ACCESSORIES FOR ELUVAC11106 Rotary evaporator bottle, NS 29/32, 100 mL, pear shaped95PRODUCT CATALOGPICKERING LABORATORIES10/11

“CUSTOMERS REALLY ONLY WANT THE CHROMATOGRAM.WE MAKE SURE THEY GET IT – GUARANTEED.”We take responsibly for all components of a method from the column, instrument and reagents.These methods are robust, rugged and very analyte selective.MARIA OFITSEROVA / SENIOR RESEARCH CHEMISTSAREETA NERKAR / RESEARCH CHEMISTAlthough multiuse, HPLC’s arevery specific when in use.

METHOD ABSTRACTS110 / AFLATOXINSTOXINS IN PEANUTS, VEGETABLE MATTER, AND MILKAatoxins occur naturally in peanuts, peanut meal, cottonseed meal, corn, dried chili pepper, etc. However, the growthof mold does not always indicate the presence of toxin since the yield of Aatoxins is dependent on growth conditionssuch as moisture, temperature, and aeration. The Aatoxins are characterized as B for blue uorescence and G for greenuorescence. The numerical subscripts indicate relative chromatographic mobility. Besides the toxins commonly found invegetable matter (B1, B2, G1, and G2), Aatoxins M (for milk) are found in milk of cows fed toxic meals. The highly toxicM metabolites are 4-hydroxylated Bs.The most important feature of the post-column method described here is that all four Aatoxins are detectable at the sameuorescence emission wavelength in a single run. The Pickering Pinnacle PCX with a 1.4 mL reactor is recommended forthis method.METHODAnalytical ConditionsColumn: MYCOTOX column, Catalog No. 1612124Temperature: 42 °CFlow Rate: 1.0 mL/minMobile Phase: MeOH, CH 3CN, H 2O; 22:22:56, IsocraticPost-column ConditionsPost-column System: Pinnacle PCX with 1.4 mL reactorTemperature: 95 °CReagent: I2 100 mg/L in waterFlow Rate: 0.31 mL/minDetection: Fluorometer, Xenon lamp ex: 365 nm, em: 430 nmG1 B2B197M2M1G2REFERENCES1) R. Buchi in “Afl atoxins,” L. Goldblat, Ed., Academic Press,New York, NY (1969)2) C.W. Thorp, G.M. Ware, and A.E. Pohland,“Proceedings of the 5thInternational IUPAC Symposium on Mycotoxins and Phycotoxins,”W. Pfannhauser and P.B. Czedic-Eysenberg (Eds.),Technical University, Vienna (1982) 52–5502 468 10 12 14min3) J.W. Dorner & R.J. Cole, J.A.O.A.C., 71 (1988) 43–474) M.J. Shepherd and J. Gilbert, Food Additives Contaminants, 1(1984) 325–335PRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS203.1 / MULTI-RESIDUE MYCOTOXIN ANALYSISSINGLE RUN ANALYSIS OF DEOXYNIVALENOL, AFLATOXINS, OCHRATOXIN A,ZEARALENONE AND FUMONISIN BY HPLC AND POST-COLUMN DERIVATIZATIONMaria Otserova, PhD, Sareeta Nerkar, PhD, Michael Pickering, PhDAlthough Aspergillus (Aatoxins, Ochratoxin A) are generally associated with peanuts and Fusarium (Deoxynivalenol,Zearalenone) with wheat, these fungi and those that produce other toxins are not host selective and so can cross plant species.This situation is complicated by the fact that the microscopic mold may not be visible to the naked eye. Also, when infectedgrains are processed, any visible mold is lost but the toxic metabolites carry over into the nished products. Thus, multi-residueanalytical screens for toxins in grain and nished goods are a wiser choice than single-family protocols. We present a singlescreen to cover ve families of toxins. This method is suitable for analyzing beverages, grains and feeds.Sample Extraction and Clean-up25 g of nely grounded sample is extracted with 150 mL of water/Methanol mixture (30/70). 20 mL of ltered extractis diluted with 70 mL of Phosphate Buffered Saline (PBS). Aatoxins, Zearealenone and Ochratoxin A are isolated usingAOZ Immunoafnity column (Vicam, USA) according to the procedure from the column manufacture. Toxins are elutedwith 2 x 2 mL of Methanol. Fumonisins are isolated using FumoniTest Immunoafnity column (Vicam, USA) according tothe procedure from the column manufacture. Toxins are eluted with 2 x 1.5 mL of Methanol.To isolate DON 3 mL of ltered extract is mixed with 6 mL of Acetonitrile and cleaned with MycoSep 277 column(Romer Labs, USA) according to the manufacture’s instructions. The cleaned solution is ltered and 5.5 mL of it is combinedwith eluants from AOZ and FumoniTest columns. The solution is evaporated to 0.5 mL and nal volume is adjustedto 1 mL with Methanol.METHODAnalytical ConditionsColumn: MYCOTOX reversed-phase C 18,4.6x250 mm Catalog No. 1612124Temperature: 40 ºCFlow Rate: 1 mL/minMobile Phase: Sodium Phosphate buffer, pH 3.5Catalog No 1700-1108/MeOH/ACNHPLC PROGRAMTIME (Min) 1700-1108 ,% METHANOL, % ACETONITRILE, %0.0 85 0 155.0 85 0 155.1 57 28 1520.0 57 28 1523.0 40 60 040.0 40 60 050.0 20 0 8060.0 20 0 80REFERENCES:Ofi tserova, M., Nerkar, S., Pickering, M., Torma, L., Thiex, N.,Multiresidue Mycotoxin Analysis in Corn Grain by ColumnHigh-performance Liquid Chromatography with Post-columnPhotochemical and Chemical Derivatization: Single-LaboratoryValidation., (2009), J AOAC Int., 92, 15-25Post-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 1.4 mLTemperature: 60 CReagent: OPA, Thiouor, Brij 35® in GA104Photochemical Reactor: UVEDetection:FluorescenceAatoxins (photochemical derivatization) ex= 365 nm; em= 455 nmFumonisins (post-column derivatization with OPA) ex= 330 nm; em= 465 nmOchratoxin A ex= 335 nm; em= 455 nmZearalenone ex= 275 nm; em= 455 nmUV/VisDeoxynivalenol=218 nm

METHOD ABSTRACTSFLD Signalreagent ONreagent OFFUV signalDONFB1reagent ONFB2G1G2 B2B1OchraZON0 10 20 30 40 50 minChromatogram of a standard solution of mycotoxins. Concentrations of toxins (ng/mL): deoxynivalenol (DON) 930,aatoxin B1 4.5, aatoxin B2 1.6, aatoxin G1 4.7, aatoxin G2 2, ochratoxin A 92, zearalenone 481, fumonisin B1474, and fumonisin B2 627.99FLD Signalreagent ONFB1reagent OFFUV signalreagent ONFB2DONG1G2 B2B1OchraZON0 10 20 30 40 50 minChromatogram of a corn grain sample naturally contaminated with fumonisins FB1 and FB2 and spiked with DON;aatoxins B1, B2, G1, and G2; ochratoxin A; and zearalenone. Concentrations of toxins in the sample (ng/g):deoxynivalenol 930, aatoxin B1 5.0, aatoxin B2 1.7, aatoxin G1 5.1, aatoxin G2 2.2, ochratoxin A 102,zearalenone 529, fumonisin B1 1838, and fumonisin B2 1107.PRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS208 / MULTI-RESIDUE MYCOTOXIN ANALYSIS OF DRY DISTILLERS GRAINSSINGLE RUN ANALYSIS OF AFLATOXINS, OCHRATOXIN A, ZEARALENONEAND FUMONISINS BY HPLC AND POST-COLUMN DERIVATIZATIONDistillers grains (DG) are the still residues after the ethanol has been collected. Approximately 90 % of US production isused in domestic animal feed. Any Mycotoxins present in the fresh corn can be concentrated by a factor of three. Contaminationcan also occur during storage. This raises concern about the potential animal and human health hazards fromthe use of Mycotoxin contaminated distillers grains. Corn entering the ethanol processing plant as well as distillers grainsshould be routinely tested for Mycotoxin contaminations to ensure compliance with guidelines set by FDA.We present a single screen method to cover 4 families of toxins that could be present in dry distillers grains (DDG).Sample Extraction and Clean-up25 g of nely grounded sample is extracted with 150 mL of water/Methanol mixture (30/70). 20 mL of ltered extract isdiluted with 70 mL of Phosphate Buffered Saline (PBS). Aatoxins, Zearealenone and OchratoxinA are isolated using AOZImmunoafnity column (Vicam, USA) according to the procedure from the column manufacture. Toxins are eluted with2 x 2 mL of Methanol. Fumonisins are isolated using FumoniTest Immunoafnity column (Vicam, USA) according to theprocedure from the column manufacture. Toxins are eluted with 2 x 1.5 mL of Methanol and combined with eluant fromAOZ column. Solution is evaporated to 0.5 mL and nal volume is adjusted to 1 mL with Methanol.METHODAnalytical ConditionsColumn: MYCOTOX reversed-phase C 18,4.6x250 mm Catalog No. 1612124Temperature: 40 ºCFlow Rate: 1 mL/minMobile Phase: Sodium Phosphate buffer, pH 3.5Catalog No 1700-1108/MeOH/ACNPost-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 1.4 mLTemperature: 60 CReagent: OPA, Thiouor, Brij 35® in GA104Photochemical Reactor: UVEDetection:FluorescenceAatoxins (photochemical derivatization) ex= 365 nm; em= 455 nmFumonisins (post-column derivatization with OPA) ex= 330 nm; em= 465 nmOchratoxin A ex= 335 nm; em= 455 nmZearalenone ex= 275 nm; em= 455 nmREFERENCES:Ofi tserova, M., Nerkar, S., Pickering, M., Torma, L., Thex, N., Multiresidue Mycotoxin Analysisin Corn Grain by Column High-Performance Liquid Chromatography with Post-columnPhotochemical and Chemical Derivatization: Single-Laboratory Validation.(2009) J.AOAC Int., 92, 15-25

METHOD ABSTRACTSFB2FB1Mycotoxin StandardOPA onOPA offOPA onOchraG1G2 B2B1Zea0 10 20 30 40 50minMYCOTOXINSPIKECONC, ppbNATURAL CONTAMINATIONLEVEL, ppbRECOVERIES % RSD N=4, %Aflatoxin B1 10.0 65 7.6Aflatoxin B2 3.4 79 6.3Aflatoxin G1 10.2 75 9.4Aflatoxin G2 4.4 82 9.1Ochratoxin A 203 89 7.1Zearalenone 1057 231 75 8.8Fumonisin B1 1042 801 109 5.8Fumonisin B2 1379 223 104 6.8101DDG Sample Spiked with MycotoxinsOPA onFB1FB2OPA offOPA onG2G1B2 B1OchraZea0 10 20 30 40 50min5-POINT CALIBRATION CURVESMYCOTOXIN CONCENTRATION RANGE CORRELATIONAflatoxin B1 0.23 – 113.1 ppb 0.99926Aflatoxin B2 0.2 – 39.7 ppb 0.99966Aflatoxin G1 0.5 – 58.2 ppb 0.99933Aflatoxin G2 0.2 – 24.7 ppb 0.99941Ochratoxin A 9.2 – 1155 ppb 0.99926Zearalenone 0.024 – 12.01 ppm 0.99908Fumonisin FB1 0.024 – 11.84 ppm 0.99987Fumonisin FB2 0.031 – 7.84 ppm 0.99993PRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS328 / ANALYSIS OF ALENDRONIC ACID IN PHARMACEUTICAL FORMULATIONSAlendronic acid or Alendronate Sodium is a bisphosphonate drug that inhibits osteoclast-mediated bone resorption.Biphosphates work by binding to the bone and preventing calcium from being removed by osteoclasts. This drug is usedto prevent and treat osteoporosis as well as several other bone diseases. Alendronate Sodium is sold as tablets or solutions,alone or in combination with Vitamin D.Since Alendronic acid has no chromophore, post-column derivatization is employed to produce a uorescence derivative.This abstract describes selective and sensitive analytical method for the analysis of Alendronic Acid in pharmaceutical tablets.Simple sample preparation and fast analysis time allow for using this method in high throughput environments.METHODAnalytical ConditionsColumn: Anion-exchange,4.1x150 mm,catalog number 1441150Temperature: 45 °CFlow Rate: 1.0 mL/minMobile Phase: Sodium Nitrate, 50 mM,pH 7.8 / Methanol (60:40)Post-column ConditionsPost-column System: Pinnacle PCXHeated Reactor Volume: 0.5 mLReactor Temperature: 70 °CReagent: OPA (300 mg), Thiouor (2g),30 % Brij 35 (3 mL) in 950 mL of OD104Reagent Flow Rate: 0.5 mL/minDetection: FLD ex: 330 nm, em: 465 nmArea20001800R 2 = 0.9982160014001200100080060040020000 200 400 600 800 1000 1200Concentration, ppmCalibration curve for analytical range 100-1000 ppmAlendronate SodiumSample PreparationCrush 1 tablet and mix with water. Use enough water to getAlendronate Sodium concentration between 300 and 1000µg/mL. Heat the solution using a water bath to 65 ºC andstir for 30 min. Filter liquid portion through 0.45 µm lter.Put ltrate in HPLC autosampler vial and inject 10 µL.0 5 10Chromatogram of Fosavance® tablet(Alendronate sodium 70 mg, Vitamin D 2800 IU)min

METHOD ABSTRACTS380 / 30-MINUTE AMINO ACID ANALYSIS OF HYDROLYZED SAMPLESPickering Laboratories specializes in the manufacturing of cation-exchange columns and eluants for amino acid analysis. Noother technique has been shown to match the reproducibility and selectivity of ion-exchange analysis with post-column Ninhydrindetection. Nor is there a chromatography technique that provides as much information; the 570/440 nm signal ratio foreach amino acid is a constant and so offers information about peak purity.Pinnacle PCX post-column derivatization system provides a unique opportunity to combine eluant gradient capabilitiesof modern HPLC instruments with column temperature gradients. As might be expected, this capability also reduces theanalysis time.We introduce an accelerated Amino Acid analysis method for hydrolyzed samples that utilizes temperature andeluant gradients.METHODAnalytical ConditionsColumn: High-efciency Sodiumcation-exchange column, 4.6 x 110 mm,Catalog number 1154110TFlow Rate: 0.6 mL/minMobile Phase: Na315, Na740, RG011Post-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 0.5 mLCOLUMN OVEN PROGRAMTIME TEMP ºC0 463 4610 5522 7027 7028 46HPLC PROGRAMTIME Na315 % Na740 % RG011 %0 100 0 07.3 100 0 015 53 47 015.1 0 100 028 0 100 028.1 0 0 10030 0 0 100103Temperature: 130 CReagent: Trione Ninhydrin reagentFlow Rate: 0.5 mL/minDetection: UV/VIS 570 nm for primary amino acids440 nm for secondary amino acidsRuntime: 30 minEquilibration Time: 9 min1 23 456 7 8129 10 15111314161718190.0 5.0 10.0 15.0 20.0 25.0minChromatogram of Protein Hydrolysate Standard1 Aspartic Acid2 Threonine3 Serine4 Glutamic Acid5 Proline6 Glycine7 Alanine8 Cystine9 Valine10 Methionine11 Isoleucine12 Leucine13 Tyrosine14 Phenylalanine15 Lysine16 Histidine17 Ammonia18 Tryptophan19 ArgininePRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS382 / 70-MIN AMINO ACID ANALYSIS OFPHYSIOLOGICAL SAMPLESPickering Laboratories specializes in the manufacturing of cation-exchangecolumns and eluants for amino acid analysis. Post-column derivatizationwith Ninhydrin offers unmatched selectivity and reproducibility of theanalysis for most challenging matrixes.Pinnacle PCX post-column derivatization system allows analysts to combineeluant gradient capabilities of modern HPLC instruments with columntemperature gradients. We introduce new accelerated Amino Acids Analysismethod for physiological samples that utilizes temperature and eluantgradients.COLUMN OVEN PROGRAMTIME TEMP ºC0 346 3417 6525 7070 7071 341HPLC PROGRAMTIME 1700-1125 % Li365 % Li375 % RG003 %0 100 0 0 010 100 0 0 019 40 60 0 032 0 100 0 043 0 100 0 043.1 0 0 100 057 0 0 100 057.1 0 0 70 3072 0 0 70 3072.1 100 0 0 0METHODAnalytical ConditionsColumn: High-efciency Lithiumcation-exchange column,4.6 x 75 mm, 5 µmCatalog number 0354675TFlow Rate: 0.55 mL/minMobile Phase: 1700-1125, Li365, Li375, RG003Post-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 0.5 mLTemperature: 130 CReagent: TrioneFlow Rate: 0.5 mL/minDetection: UV/VIS 570 nm for primary amino acids440 nm for secondary amino acidsRuntime: 72 minEquilibration Time: 15 min4320222357 8 111091315171618 211924232931343335363837394225 2627 28461214:303240 410 10 20 30 40 50 60 70min1 Phosphoserine2 Taurine3 Phosphoethanolamine4 Urea5 Aspartic acid6 Hydroxyproline7 Threonine8 Serine9 Asparagine10 Glutamic acid11 Glutamine12 Sarcosine13 -Aminoadipic acid14 Proline15 Glycine16 Alanine17 Citrulline18 -Amino-n-butyric acid19 Valine20 Cystine21 Methionine22 Cystathionine23 Isoleucine24 Leucine25 Tyrosine26 Phenylalanine27 -Alanine28 -Amino-i-butyric acid29 Homocystine30 -Aminobutyric acid31 Tryptophan32 Ethanolamine33 Ammonia34 Hydroxylysines35 Ornitine36 Lysine37 1-Methylhistidine38 Histidine39 3-Methylhistidine40 Anserine41 Carnosine42 Arginine43 Glucosaminic Acid**Internal Standard

MetTyrPheLeu + IleMETHOD ABSTRACTS107 / PKU AND MSUDSCREENING FOR PHENYLKETONURIA AND MAPLE SYRUP URINE DISEASEPhenylketonuria (PKU) and Maple syrup urine disease (MSUD, branched-chain ketoaciduria) are genetically transmittedmetabolic diseases. PKU results in elevated Phenylalanine levels due to decient activity of the enzyme Phenylalaninehydroxylase. In the MSUD state, elevated levels of leucine (Leu), isoleucine (Ile), valine (Val), and alloiso-leucine can bedetected in blood. The increased concentration of the branched-chain amino acids is caused by an inherited decient activityof the enzyme branched-chain keto-acid decarboxylase.Failure to diagnose and treat these conditions in newborns can result in severe mental retardation (PKU) or even death (MSUD).Dietary restriction of Val, Leu, and Ile for MSUD newborns must begin immediately once the diagnosis is made or suspected.MSUD patients must be monitored on a regular schedule in conjunction with the dietary therapy because evenrelatively small changes in the intake of the branched-chain amino acids may produce rather large uctuations of their concentrationsin the plasma. In this respect, MSUD is more difcult to control by dietary means than phenylketonuria (PKU),because each of the branched-chain amino acids has to be individually adjusted and monitored.The Pickering Laboratories PKU/MSUD post-column system is a rapid, automated method for the quantitative analysis ofMet, Leu + Ile, Tyr and Phe from whole blood samples. Between-injection time is 13 min while actual time is only 7 min.The Pickering lithium ion-exchange column has a matched set of eluant and regenerant to provide the best possible separation,sensitivity, and reproducibility. The post-column derivatizing reagent is TRIONE® Ninhydrin.METHODAnalytical ConditionsColumn: PKU/MSUD column, 4.0 x 50 mmCatalog No. 0354050Temperature: 65 °CFlow Rate: 0.4 mL/minMobile Phase: Eluant (A): Li357, Regenerant (B): RG003Gradient:0–8.0 min 100 % A8.1–10.0 min 100 % B10.1–13.0 min 100 % APost-column ConditionsPost-column System: Pinnacle PCXReaction Dwell: 0.5 mLTemperature: 130 °CReagent: TRIONE® NinhydrinFlow Rate: 0.3 mL/minDetection: Visible @ 570 nm01 23Neonate serumwith elevatedPhenylalanineNormalNeonate serum4 5 6 7 8minThis chromatogram is reproduced by courtesy of Quynh Vantu and Wanda Andrews, VirginiaNewborn Screenng Laboratory, Richmond, Virginia.105“TRIONE” (U.S. Patent No. 4,274,833) is a registeredtrademark of Pickering Laboratories, Inc.PRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS205 / AMINOGLYCOSIDE ANTIBIOTICS IN FEEDAPRAMYCIN, GENTAMICIN, NEOMYCINAminoglycoside antibiotics are among those commonly used in animal feeds to manage intestinal microorganisms. Thebenecial effects include improved growth and generally healthier animal populations. Use of antibiotics creates a demandfor analytical procedures to verify concentrations in pre-mixes and feeds and in some instances for residue analysis in animalproducts.This note describes a simple, robust analytical method for the family of Aminoglycoside antibiotics in feeds and animalproducts. The sample is homogenized with a generic extraction solution and the crude soluble portion is directly injectedinto an HPLC ion-exchange column. The column efuent is then mixed with an OPA/Thiouor reagent which formshighly uorescent derivatives with the primary amine moieties of the antibiotics.Call if you need to analyze other Aminoglycosides.Extraction Procedure:Take one part feed:10 parts Extraction solution (w/v)Catalog No. 1700-1118 and homogenize for ve minutes.Centrifuge for 10 minutes. Three layers will form: the pellet,a supernatant emulsion and a soft layer of oating fat. Carefullylift the oating fatty layer with a spatula and discard.Transfer the emulsion to a sealable vial. Coagulate theemulsion by placing the vial in a boiling water bath for 15minutes. Centrifuge for 10 minutes. The clear supernate isltered (0.45 um Nylon) and placed in an autosampler vial.METHODAnalytical ConditionsColumn: ALKION cation-exchange, K+ form, 4 x 150 mm,Catalog No. 9410917ALKION Guard column, 3 x 20 mm,Catalog No. 9493020ApramycinNeomycinGentamicinComplexTemperature: 40 °CFlow Rate: 0.8 mL/minMobile Phase:1700-1101, Potassium buffer, K011700-1102, Potassium titrant, K021700-1103, Potassium ionic strength adjuster, K03Post-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 0.15 mLTemperature: 45 °CReagent: o-Phthalaldehyde/Thiouor + Brij 35®Flow Rate: 0.3 mL/minDetection: Fluorometer ex: 330 nm, em: 465 nm“ALKION” and Thiofl uor are trademarks of Pickering Laboratories, Inc.“Brij 35” is a registered trademark of ICI Americas, Inc.05 10 15 20 25 30minCONDITIONS FOR SEPARATION OF APRAMYCINGENTAMICIN AND NEOMYCINTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 60 40 015 13.2 8.8 7815.1 12 10 7830 12 10 7830.1 0 22 7831 0 22 7831.1 60 40 037 60 40 0FLOW RATE: 0.8 mL/min,COLUMN TEMP.: 40 ºC,INJECTION VOLUME: 10 LREFERENCES:Driver, J. L., Thex, N., Raynie, D., Ofi tserova, M., Pickering, M. Single-Laboratory Validation forthe Quantifi cation of Neomycin B and Neomycin C in Animal Feeds by Liquid ChromatographyFluorescence Detection with Post-column Derivatization.(2009) J.AOAC Int, 92, 34-41

METHOD ABSTRACTSStandard obtainedfrom SigmaApramycinAPRAMYCIN ANALYSISTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 67 33 05 67 33 015 14.7 7.3 7820 14.7 7.3 7820.1 0 22 7821 0 22 7821.1 67 33 0SAMPLE: 11.1 nmole Apramycin on column010 20 30minStandard obtainedfrom SigmaGentamicin Complex{GENTAMICIN ANALYSISTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 43 31 2620 9 13 7830 9 13 7830.1 0 22 7831 0 22 7831.1 43 31 26SAMPLE: 5 x 10-6 g Gentamicin complex on column107010 20 30minStandard obtained fromUS PharmacopeiaNeomycinNEOMYCIN ANALYSISTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 %0 60 40 015 13.2 8.8 7825 11 11 7825.1 0 22 7826 0 22 7826.1 60 40 0SAMPLE: 2.75 nmole Neomycin010 20 30minPRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS102 / ANALYSIS OF BIOGENIC AMINESFOOD AND PHYSIOLOGICAL SAMPLESHistamine and other polyamines such as Putrescine, Cadaverine, Spermidine and Spermine are products of decomposition.The level of histamine, in particular, has been used as a regulatory guideline for degree of decomposition in sh. Also,Histamine and Tyramine levels are of interest to producers of wine and cheese.The polyamines, Spermidine, Spermine and Putrescine are elevated in neoplastic and damaged, e.g., burned tissue. In fact,the level of circulating and urinary polyamines is an indicator of total body damage. The levels can be monitored for theeffect of diet and other modalities on recovery.This method employs ion-exchange chromatography so, after extraction, centrifugation/ltration are the only samplepreparations necessary.Extraction ProcedureWeigh 10 g of sample into a small glass blender cup (anyfortications should be added at this time). Add 50 mL ofextraction solution, 80 % HPLC Methanol and 20 % 0.1N Hcl, and homogenize for two minutes. Centrifuge forve minutes. Mix equal portions of the supernatant withthe mobile phase (K600). Allow to coagulate at -4 °C for 15minutes, then centrifuge for ve minutes. The clear supernatantis ltered (0.45 µm Nylon) and placed in an autosamplervial.METHODAnalytical ConditionsColumn: ALKION cation-exchange, K+ form,4 x 150 mm, Catalog No. 9410917ALKION Guard column, 3 x 20 mm,Catalog No. 9493020Temperature: 45 °CFlow Rate: 0.8 mL/minMobile Phase: K600, K563, K130Post-column ConditionsPost-Column System: Pinnacle PCXReactor Volume: 0.15 mLTemperature: 45 °CReagent: 300 mg of OPA, 2 g of Thiouor,3 mL of 30 % Brij® 35 in 950 mL of OD104Flow Rate: 0.3 mL/minDetection: Fluorometer ex: 330 nm, em: 465 nm

METHOD ABSTRACTSHistamine5 Days Old5 Days OldFreshPutrescineCadaverineHistamineFreshPutrescineCadaverineTIME (Min) K600 % K130 %0 100 012 100 012.1 0 10015 0 10015.1 100 020 100 002 4 6 8 10Ahi Tunamin02 4 6 8 10Mahi-Mahimin1 Histamine2 Putrescine3 Cadaverine1093 425015 10 15 20minGradient Separation of Polyamine MixtureTIME (Min) K600 % K563 % K130 %0 100 0 06 100 0 015 0 100 021 0 100 021.1 0 0 10023 0 0 10023.1 100 0 029 100 0 01 Histamine2 Putrescine3 Cavaverine4 Spermidine5 SperminePRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS120 / BROMATE ANALYSISANALYSIS OF BROMATE IN DRINKING WATER BY HPLC AND POST-COLUMN DERIVATIZATIONBromate is a disinfection by-product that is formed when Ozone reacts with naturally occurring Bromide in drinkingwater. Bromate is a known animal carcinogen and has also been listed as a group 2B toxin: probable human carcinogen.The U.S.EPA Method 300.1 employs conductivity as the means of detection which works well for most anions. However,the method is non-specic and coeluting interferences cannot be identied. The more recent U.S.EPA Method 317.0utilizes a Bromate specic reagent in a post-column reaction. This allows for a very specic and sensitive assay forBromate in complex matrices.METHODEquipment• LC with a binary pump• UV/VIS detector• Pickering Laboratories dual reagent PCX5200 Post-columnderivatization instrument• Pickering Laboratories anion-exchange column, 4.6 x 150mm(Cat. No. 0785150)Reagents• 9.0 mM Sodium carbonate• Conc. Nitric acid (70 %)• Potassium bromide• o–Dianisidine dihydrochloride• MethanolLC ConditionsLC Column Temperature: 42CSample Injection Volume: 250 µLLC Flow Rate: 1.3 mL/minMobile Phase: 9 mM Na 2CO 3Post-column ConditionsPost-column system: Pinnacle PCXReactor Volume: 0.5 mLReactor Temperature: 60CReagent:o–Dianisidine dihydrochloride (Add 40 mL of 70 % HNO 3to 300mL deionized water in a 500mL volumetric ask. Dissolve2.5g KBr in this solution. Dissolve 250 mg ofo–Dianisidine dihydrochloride in 100 mL of Methanol andadd to the Nitric acid/KBr solution and dilute to volume.)Flow Rate: 0.7 mL/minDetection: UV/VIS detector, max = 450 nmREFERENCESU.S.EPA Method 317.0. Determination of inorganic oxyhalide disinfection by-products indrinking water using ion chromatography with the addition of a post-column reagent for tracebromate analysis.H.P.Wagner, B.V.Pepich, D.P.Hautman and D.J.Munch, J.Chromatography A, 882 (2000)309 – 319.C.R.Warner, D.H.Daniels, F.L.Joe and G.W.Diachenko, Food Additives and Contaminants,vol. 13, No.6 (1996) 633 – 638.

METHOD ABSTRACTS90007000y = 705x + 6.6128R 2 = 0.9998Area5000300010002 6 10Concentration (ppb)Calibration CurveuAU2.010ppb1.58ppb1.00.55ppb1110.02.5ppb-0.41.50 1.75 2.00 2.25 2.50 2.75 3.00minPRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS108 / POST-COLUMN LC ANALYSIS OF 23 N-METHYL CARBAMATESAN EXPANDED METHOD USING WATER/ACETONITRILE OR WATER/METHANOL GRADIENTSThere are a number of carbamate pesticide compounds employed worldwide which are not included in the 10 compoundsmandated by USEPA Method 531.1 and AOAC Protocol 29.A05.This Application Note describes methods for separating as many as 23 compounds, using a Pickering C8, 5 µm silica, columnwith either Water/Acetonitrile or Water/Methanol gradients. Instruments, post-column derivatization and detectionprotocols, and reagents remain unchanged, as described Pickering’s brochure, Post-column LC Systems for Environmental PesticideAnalysis (B-CA5) and User’s Manual 0101-0002.Although there are only two co-elutions in each of the solvent gradient protocols, the compounds were assigned to twoseparate test mixtures, A and B, in order to demonstrate the full range of separation.The differences in selectivity between the Acetonitrile and Methanol protocols enable the user to employ both solventsystems as a means of conrming peak identication.The following comparison of Acetonitrile to Methanol might be helpful in choosing which solvent system will be for usedroutinely, and which will be for conrmation:• Acetonitrile generally exhibits higher sensitivity and baseline noise.• It also costs more and its disposal is more restricted.Eleven of the most widely occurring carbamate compounds and BDMC (internal standard) are supplied by PickeringLaboratories in its qualitative test mixtures, Cat. No. 1700-0063. These are designated by * in the test mixture tables below.Other compounds may be purchased from suppliers of environmental standards. A few may vary considerably in homogeneityand availability.Water/AcetonitrileWater/Methanol1 Aldicarb sulfoxide (Standak)2 Aldicarb sulfone3 Oxamyl (Vydate)4 Methomyl (Lannate)5 3-Hydroxy carbofuran6 Aldicarb (Temik)7 Propoxur (Baygon)8 Carbofuran (Furadan)9 Carbaryl (Sevin)10 Methiocarb (Mesurol)11 BDMC internal standard12 Butocarboxim sulfoxide13 Butocarboxim sulfone14 Ethiofencarb sulfoxide15 Ethiofencarb sulfone16 Butocarboxim17 Ethiofencarb18 Thiofanox Sulfoxide19 Thiofanox Sulfone20 Methiocarb sulfoxide21 Methiocarb sulfone22 3-Ketocarbofuran23 Thiofanox

METHOD ABSTRACTSAnalytical Conditions: Water/Acetonitrile ProtocolColumn: 4.0 x 250 mm, Pickering Cat.No. 0840250Sample Injection: 10 µl in MeOH (for all chromatograms shown)Mobile Phase:Solvent A: HPLC-grade WaterSolvent B: HPLC-grade Acetonitrile (ACN)Program:TIME H 2O % ACN % GRADIENT0 90 102 90 10 Isocratic46 49 51 Linear46.1 30 70 Step49 30 70 IsocraticEquilibration Time: 13 minutesEluant Flow Rate: 0.80 mL/minuteColumn Temperature: 37 CReagent 1: 0.3 mL/minute, CB130 Hydrolysis ReagentReagent 2: 0.3 mL/minute, OPA reagent 1 for derivatizationReactors:Hydrolysis: 100 C, 500 µLDerivatization: Ambient, 100 µLFlourescence Detection:Excitation: 330 nmEmission: 465 nmAnalytical Conditions: Water/Methanol ProtocolMobile Phase:Solvent A: HPLC-grade WaterSolvent B: HPLC-grade Methanol (MeOH)Program:TIME H 2O % MeOH % GRADIENT0 88 122 88 12 Isocratic42 34 66 Linear46 34 66 Isocratic46.1 0 100 Step49 0 100 Isocratic1131 o-Phthalaldehyde (O120) and Thiofl our (3700-2000), Pickering’sbrand of Dimethylaminoethanethiol•HCI, in OPA Diluent (CB910)REFERENCE:R.T. Krause, J. Chromatography, 185, 615-624 (1979)R.T. J. Chromatographic Science, 16, 281-288 (1978)H.A. Moye, S.J. Scherer and P.A. St. John, Anal. Letters, 10, 1049-1073 (1977)A. de Kok and M. Hiemstra, J. AOAC int’l, 75, 1063-1072 (1992)Offi cial Methods of Analysis, 15th Ed., AOAC Int’l, Arlington, VA, (291-293)Method 531.1, U.S. Environmental Protection AgencyPRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS112 / EXPANDED HPLC METHOD FOR N-METHYL CARBAMATESPOST-COLUMN ANALYSIS OF PESTICIDES IN FOOD AND POTABLE WATER SAMPLESCarbamate pesticides are widely used around the world to protect crops. In addition, they are used as biocides for industrialor other applications and in household products. Though carbamates are biodegradable compounds and bioaccumulationusually happens only to a low extend it is important to monitor produce to make sure enough time has elapsed betweenharvest and applying pesticides. Also, because of their high solubility, carbamates can leach into ground waters in poroussoils and consequently nd their way into drinking water supplies.As part of FDA’s pesticide monitoring program individual lots of domestic and imported foods and feeds are sampled andtested for pesticide residues to enforce the tolerances set by EPA. There are 11 compounds mandated by USEPA Method531.2 for drinking water but they represent only a fraction of the carbamates that require monitoring in domestic andimported products. Methyl carbamates are separated using a reversed-phase column and then readily react with o-Phthalaldehydeand a mercaptan after hydrolysis to form highly uorescence compounds. This post-column reaction is the basis forofcial EPA Method 531.2 and AOAC Method 985.23.This new expanded method is suitable for detecting a wide range of carbamates; post-column derivatization with uorescencedetection is a sensitive and selective method for residue analysis in water, food and feed samples. This methodemploys the same HPLC and post-column equipment and chemicals as USEPA Method 531.2 and will allow laboratoriesto increase the range of tested compounds.The separation is achieved on a C 8stationary phase with a water/Methanol gradient. Differences in selectivity of a water/Acetonitrile gradient may be used for conrmation.METHODAnalytical ConditionsColumn: Expanded resolution C 8analytical column 4x250 mm,P/N 0840250 Guard column P/N 18ECG001Flow Rate: 0.8 mL/minColumn Temperature: 50 CMobile Phase: Water/MethanolPost-column ConditionsPost-column System: Pinnacle PCX or Vector PCXReactor Volume: 0.5 mLReactor Temperature: 100 CReagent 1: CB130Reagent 2: 100 mg of OPA and 2 g of Thiouor in 950 mLof CB910 DiluentReagents Flow Rate: 0.3 mL/minDetection:Fluorescence Detector ex= 330 nm, em= 465 nmHPLC Gradient:TIME WATER % METHANOL %0 85 152 85 1542 30 7046 30 7046.1 0 10050 0 100Equilibration: 10 minACKNOWLEDGEMENTS:Pickering Laboratories would like to thank John Casanova and Frank Schenck (FDA) fortheir help with this project.

METHOD ABSTRACTS1 35678 915192021222726 28293031320 10 20 30 40min24101112 13 1416171823242533 34350 10 20 30 40 min11551522351 2 3768 920212633 3230310 10 20 30 40 minList of Carbamates1 Aldicarb sulfoxide2 Butoxycarboxim3 Aldicarb sulfone4 Oxamyl5 Methomyl6 Ethiofencarb sulfone7 Ethidimuron8 Thiofanox sulfoxide9 Thiofanox sulfone10 Formetanate HCl11 Ethiofencarb sulfoxide12 Dioxacarb13 3-Hydroxycarbofuran14 Butocarboxim15 Aldicarb16 Metolcarb17 Cloethacarb18 Bendiocarb19 Carbetamide20 Propoxur21 Carbofuran22 Carbaryl23 Ethiofencarb24 Thiofanox25 Banol26 Isoprocarb (MIPC)27 2,3,5-Trimethacarb28 3,4,5-Trimethacarb29 Fenobucarb (BPMC)30 Methiocarb31 BDMC32 Bufencarb33 Promecarb34 Zectran35 AminocarbPRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS325 / ANALYSIS OF FORMALDEHYDESIMPLE AND SENSITIVE METHOD FOR ANALYZING FREE FORMALDEHYDEFormaldehyde is used for disinfections of industrially manufactured products. However, it is detrimental to health and itspotential oncogenicity led to the current utilization of a formaldehyde donor for long-term preservation. Such donors are inhydrolytic equilibrium with formaldehyde producing free formaldehyde on exposure to moisture.This method is used to quantify the free formaldehyde without upsetting the existing equilibrium. Potential interferencesare either separated from formaldehyde or they don’t react with the post-column reagent. The lutidine derivative is highlyuorescent resulting in ppm detection levels.METHODAnalytical ConditionsColumn: Diol, 5 µm, 4.6 x 250 mmTemperature: 40 CFlow Rate: 0.5 mL/minMobil Phase: Acetonitrile: Water (85:15) isocraticPost-column ConditionsPost-column System: Pinnacle PCXReactor Volume: 0.5 mLTemperature: 100 CReagent:0.81 M Ammonimn acetate0.12 M Galcial acetic acid (pH=5)0.05 M 2, 4-Pentanedione with waterFlow Rate: 0.4 mL/minDetection: Fluorometer ex: 412 nm, em: 510 nm4.0 6.0 8.0 10.0 12.0 14.0 16.0

METHOD ABSTRACTS206 / GLYPHOSATE AND AMPA ANALYSIS IN CROPSA SIMPLE AND REPRODUCIBLE EXTRACTION AND CLEAN-UP 2 FOR HPLC POST-COLUMN DERIVATIZATIONThe recently practiced method1 for analysis of Glyphosate and AMPA in crops suffers from an expensive, time consumingclean-up procedure that has less than ideal recoveries. Although the analysis (after clean-up) by ion-exchange chromatographywith post-column derivatization is rugged and sensitive, a new method was sought to improve the sample preparation.This resulted in AOAC Method 2000.52 2 which has a streamlined clean-up followed by pre-column derivatization andGC/MS analysis. We show how this simplied sample preparation is suitable for the classic ion-exchange/post-columnanalytical protocol.METHODEquipment:• LC with a binary pump• Fluorescence detector• Pickering Laboratories Vector PCX or Pinnacle PCX Post-columnDerivatization instrument• Pickering Laboratories Potassium cation exchange column,4.0 x 150 mm (Cat. No. 1954150)• Pickering Laboratories Potassium cation exchange guard column,3.0 x 20 mm (Cat. No 1953020)• Cation exchange clean-up column (Cat. No 1705-0001)Reagents:• Potassium eluant (Cat. No.K200)• Potassium regenerant (Cat. No. RG019)• Hypochlorite diluent (Cat. No. GA116)• o-Phthalaldehyde diluent (Cat. No. GA104)• Thiofluor (Cat. No. 3700-2000)• o-Phthalaldehyde (Cat. No. O120)• 5 % Sodium hypochlorite solution• Methylene chloride• Acidic modifier solution• Clean-up column eluantSample PreparationExtraction:To 25 g of a homogenous sample add enough water (afterestimation of moisture content) to make the total volume ofwater 125 mL. Blend at high speed for 3–5 min. and centrifugefor 10 min. Transfer 20 mL of the aqueous extract intoa centrifuge tube and add 15 mL of methylene chloride (toremove nonpolar co-extractives). Shake for 2–3 min. andcentrifuge for 10 min. Transfer 4.5 mL of the aqueous layerinto a vial and add 0.50 mL acidic modier solution (16gKH 2PO 4, 160 mL H 2O, 40 mL Methanol, 13.4 mL HCl).Shake and centrifuge for 10 min.Matrix Speci c Modi cation:Plants with high: 1) Water 2) Protein 3) Fat Content1 For crops that absorb large amounts of water, reduce testportion to 12.5 g keeping water volume the same.2 For crops that have high protein content add 100 µL HCl to20 mL aliquot of crude extract. Cap, shake and centrifugefor 10 min.3 For crops that have high oil content, do the methylenechloride partition twice.Cation-Exchange Clean-up:Transfer 1 mL of extract (representing 0.18 g normal cropor 0.09 g dry crop) to the column reservoir and elute to thetop of the resin bed. Add 0.70 mL of the elution solution(160 mL H 20, 2.7 mL HCl, 40 mL Methanol) and discardthe efuent. Repeat with a second 0.70 mL portion anddiscard efuent. Elute with 12 mL of the elution solutionand collect in a round-bottomed ask. Evaporate to drynessin a water bath set at 40 C using a rotary evaporator. Orcollect in a centrifuge tube and evaporate using a vacuumvortex evaporator. Dissolve residue in 2.0 mL of the elutionsolution (use 1.5 mL for dry crops). Extracts before evaporationcan be stored refrigerated for up to 7 days.117Continued on next pagePRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS206 continued from previous pageLC ConditionsLC Column Temperature: 55 CSample Injection Volume: 100 µLLC Flow Rate: 0.40 mL/minMobile Phases:K200, Potassium EluantRG019, RegenerantPost-column ConditionsPost-column System: Vector PCX or Pinnacle PCXReactor Volume: 0.5 mLReactor Temperature: 36 CReagent 1: 100µL of 5 % NaOCl (Bleach) in GA116 DiluentReagent 2: 100 mg o-Phthalaldehyde and 2g Thiouor in950 mL GA104 DiluentFlow rate: 0.3 mL/minDetection: Fluorescence detector ex: 330 nm, em: 465 nmACKNOWLEDGEMENT:We thank The Montana Department of Agriculture, Laboratory Bureau for extractingalfalfa, tomato and other samples.REFERENCES:1) “Validation of an Analytical Residue Method for Analysis of Glyphosate and Metabolite:An Interlaboratory Study.” J. Agric. Food Chem. 34, (1986) 955–960.2) P.L. Alferness and L.A. Wiebe, “Determination of Glyphosate and AminomethylphosphonicAcid in Crops by Capillary Gas Chromatography with Mass-Selective Detection:Collaborative Study.” Journal of AOAC International, 2001 84, 823–846.STEP TIME (Min) INTERVAL (Min) K200 % RG019 %0 0 0 100 0 Injection1 0-15 15 100 0 Isocratic2 15.1-17 1.9 0 100 Step Change3 17.1-25 7.9 100 0 Re-EquilibrationNOTE: Ensure that the auto sampler injection valve is fi tted with a Tefzel or PEEK rotorseal for compatibility with the high-pH column regenerant.Heavy-metal ions, especially iron can cause loss of sensitivity. Contamination of the guardand column can be removed by using RESTORE (Cat. No. 1700-0140) reagent.GlyphosateAMPAAlfalfa spiked with0.2 ppm glyphosateTomato spiked with0.15 ppm glyphosate2 4 6 8 10 12 14 16 18 20 22 24Time [min]Chromatograms of Alfalfa and Tomato matrix spiked with glyphosate and AMPA

METHOD ABSTRACTS123 / SIMULTANEOUS DETERMINATION OF NITRITE AND NITRATE IN PROCESSED FOODSSIMULTANEOUS DETERMINATION OF NITRITE AND NITRATE IN BABY FOODSSaji George, Maria Otserova, Ph. D., Michael Pickering, Ph. D.Nitrite either alone or in combination with nitrate is added to food to preserve the color and taste and to prevent foods frombecoming rancid. They are also used in food for their anti-microbial properties. Higher levels in vegetables and leafy greensare possible from the use of nitrate fertilizers and/or livestock manure.Nitrate can be reduced to nitrite at certain physiological conditions in the human body. Nitrite however can oxidize Fe (II) inhemoglobin to methemoglobin, an Fe (III) product. The oxidized product is incapable of binding molecular oxygen andhigh concentrations of methemoglobin can result in methemoglobinemia especially in infants. Nitrite can also react withsecondary amines present in food products or in the digestive system to form nitrosamines, a class of carcinogenic compounds.Nitrite levels in food could also be produced by reduction of nitrate to nitrite during processing.The AOAC Ofcial Method 993.03 1 for the analysis of nitrate involves reduction using spongy Cadmium which is toxic andcarcinogenic. The USFDA improved on this method by using Vanadium (III) chloride and heat 2 for the post-column reductionof nitrate to nitrite. Nitrite reacts with this modied Griess reagent to produce a red chromophore with maximal absorbanceat 535 nm. Pickering Laboratories Inc. has further improved this method by substituting the corrosive and volatilehydrochloric acid with methane sulfonic acid.METHODEquipment• LC with a binary pump• UV/VIS detector• Pickering Laboratories single reagent Pinnacle PCXpost-column derivatization unit(1153-1021 - 120 V, 1153-1022 – 240 V)• Pickering Laboratories anion exchange column,4.6 X 150 mm (Cat. No. 0785150)Chemicals• Sodium acetate• Vanadium(III)chloride• N-(1-Naphthyl)ethylenediamine dihydrochloride• m-Nitro aniline• 20 %(v/v) methane sulfonic acidLC ConditionsSample Injection Volume: 10 µLLC Flow Rate: 1 mL/minMobile Phase: 0.15 M sodium acetatePost-column ConditionsColumn Temperature: 50 °CReactor Volume: 0.5 mLReactor Temperature: 100 °CReagent Flow Rate: 0.1 mL/minReagent:Mix 50 mL of (i) and (ii), and 1.25 mL of (iii) and dilute to250 mL using 20 % methanesulfonic acid[ (i) 1 % Vanadium(III)chloride in 20 % methanesulfonicacid,(ii) 1 % m-Nitro aniline in 20 % methanesulfonic acid,(iii) 1 % N-(1-Naphthyl)ethylenediamine dihydrochloride in20 % methanesulfonic acid ]Detector: UV/VIS, max= 535 nmExtraction ProcedureFruits and VegetablesTo 5 g of baby food in a 50 mL centrifuge tube add 25mL of 50-60 ºC water (for vegetables) or 15 mM Sodiumacetate (for fruits) and shake for 10 min. Add 12.5 mL ofacetonitrile and make up the volume to 50 mL using water(for vegetables) and sodium acetate (for fruits). Centrifugethe mixture for 15 mins at 5000 rpm. Filter the supernatantthrough a 0.45 µ nylon lter and dilute to fall within thelinear range.Processed MeatTo 5 g of homogenized processed meat in a blender add 25mL of 50-60 ºC water and blend for 2 min. Add 25 mL ofacetonitrile and blend for an additional 2 min. Transfer intoa beaker and make up the volume to 100 mL using warmwater. Filter themixture using Whatman lter paper. Filter further through a0.45 µ nylon lter and dilute to fall within the linear range.NOTESPost-column reagent solutions are stored in plastic containers.All solutions are fi ltered through 0.45 µ nylon fi lter before use.Nitrate/Nitrite standards should be checked prior to use for oxidation.Sample pH should be checked to determine the choice of extraction solutionsince acidic pH facilitates the conversion of nitrite to nitrate.119Continued on next pagePRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS123 continued from previous page10 ppm nitrite-nitrate standard using (a) 20 %methanesulfonic acid , (b) 20 % hydrochloric acidNitriteNitratea)b)0 10 20min(a) 0.05 ppm, (b) 0.01 ppm nitrite-nitrate standardNitritea)b)Nitrate0.0 5.0 10.0 15.0 minCalibration Curve for Nitrite-Nitrate (0.05 ppm – 50 ppm)2500000020000000y (nitrite) = 377491x + 204536R 2 = 0.99861500000010000000y (nitrate) = 166790x - 17901R 2 = 0.9985500000000NitriteNitrate10 20 30 40 50 60

METHOD ABSTRACTSBanana (baby food): (a) spiked with 100 ppmnitrite-nitrate solution, (b) blankCarrot (baby food): (a) spiked with 100 ppmnitrite-nitrate solution, (b) blankNitrateb)a)Nitratea)Nitriteb)0.0 5.0 10.0 15.0 minNitriteCorned Beef: (a) spiked with 50 ppm nitrite and500 ppm nitrate solution, (b) blank0.0 5.0 10.0 15.0 minNitratea)b)Nitrite0.0 5.0 10.0 15.0 min121SAMPLESPIKEDCONC.(PPM)NITRITECAL.CONC.(PPM)RECOVERY DATARECOVERY(%)SPIKEDCONC.(PPM)NITRATECAL.CONC.(PPM)RECOVERY(%)Baby FoodCarrot 100 92.25 92 100 79.3 79Banana 100 94.6 95 100 102.5 103ProcessedFoodCornedBeef50 54.4 109 500 478.9 96ACKNOWLEDGMENTSJohn A. Casanova, Food and Drug Administration, 60 8 th Street, Atlanta, GA 30309REFERENCES1. 1. AOAC- Offi cial Methods of Analysis of AOAC International (2000) 17th Ed., Section 50.1.11.2. Use of Griess Reagents Containing Vanadium (III) for the Post-columnDerivatization and Simultaneous Determination of Nitrite and Nitrite in Baby Food,John A. Casanova, Lois K. Gross, Sarah E. McMullen and Frank Schenck,Food and Drug Administration, 60 8th Street, Atlanta, GA 30309.PRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS105.2 / PARALYTIC SHELLFISH TOXINSANALYSIS OF DINOFLAGELATE DERIVED NEUROTOXINS IN BIVALVE MULLUSKS USINGHPLC POST-COLUMN FLUORESCENCE METHODThe paralytic shellsh toxins are a group of 18 secondary metabolites deposited in bivalve mollusks by dinoagelates. Dino-agelates blooms are seasonal, occurring during warm months. Since it is unpredictable whether an infestation will occur,the shellsh population should be regularly monitored for toxins. Ingestion of contaminated shellsh can lead to paralyticshellsh poisoning; a life-threatening illness.Mouse bioassay is the ofcial method of AOAC International, but the drawbacks associated with this method have led toexploration of chemical methods. The most common HPLC post-column method is to oxidize the separated toxins underalkaline conditions to a uorescent compound. Sullivan et al. 1 used this method to determine the gonyautoxins 1-6 (GTX1-6), saxitoxin (STX) and neosaxitoxin (neoSTX) but not the N-sulfocarbamoyl-11-hydroxysulfate toxins (C1-C4). Oshima etal. 2, 3 modied this method to determine the 3 toxin groups separately using isocratic elution with 3 different mobile phases.Further improvement by Jeffery van de Riet et al. 4, 5 has led to a shorter analysis time to determine the 3 groups of toxinsusing step gradient and a switching valve. This method abstract describes the use of Pickering Laboratories Pinnacle PCXpost-column derivatization system for the HPLC post-column determination of paralytic shell sh toxins.METHODEquipment:• LC with a Binary Pump• Fluorescence Detector• Pickering Laboratories dual reagentPinnacle PCX post-column derivatization unit(1153-1061 – 120 V, 1153-1062 – 240 V)• Agilent Technologies Zorbax Bonus RP column,3.5 µm, 4.6 x 150 mm (863668-901)Reagents:• Heptane Sulfonate• Phosphoric Acid• Ammonium Hydroxide• Acetonitrile• Periodic Acid• Sodium Hydroxide• Nitric AcidLC ConditionsSample Injection Volume: 10 µLFlow Rate: 0.8 mL/minMobile Phase:A. 11 mM heptane sulfonate, 5.5 mM phosphoric acid, adjustedto pH 7.1 with ammonium hydroxide.B. 11 mM heptane sulfonate, 16.5 mM phosphoric acid, 11.5 %acetonitrile, adjusted to pH 7.1 with ammonium hydroxide.Post-column ConditionsColumn Temperature: 40 °CReactor Volume: 1.0 mL (knitted)Reactor Temperature: 85 °CReagent Flow Rate: 0.4 mL/minReagents:1. 100 mM phosphoric acid, 5 mM periodic acid,adjusted to pH 7.8 with 5 M sodium hydroxide2. 0.75 M nitric acidDetection: Fluorometer ex: 330 nm, em: 390 nm

METHOD ABSTRACTSGTX4GTX1dcGTX3GTX5 dcGTX2neoSTXdcSTXSTXGTX2GTX30.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00Chromatogram of GTX and STX mixed working standardHPLC GRADIENT PROGRAMTIME (Min) %A %B0 100 07.9 100 08 0 10018.5 0 10018.6 100 024 100 0123REFERENCES:1) J.J.Sullivan et. alJ.Food Sci. 50(1) (1985) 26-29.2) Yasukatsu Oshima“Post-column Derivatization Liquid Chromatographic Method for Paralytic Shellfi sh Toxins.”Journal of AOAC International Vol.78, No.2, (1995) 528-532.3) Yasukatsu Oshima, et. alIn Mycotoxins and Phytotoxins “88, Elsevier Science Publishers,Amsterdam, The Netherlands, 319-326.4) Wade A. Rourke, et. al“Rapid Post-column Methodology for Determination of Paralytic Shellfi sh Toxins in Shellfi sh Tissue.”Journal of AOAC International Vol.91, No.3, (2008) 589-597.5) Jeffrey M. van de Riet, et. al“Liquid Chromatographic Post-column Oxidation Method for Analysis of Paralytic Shellfi sh Toxins inMussels, Clams, Scallops and Oysters: Single-Laboratory Validation:”Journal of AOAC International Vol.92, No. 6, (2009) 1690-1704.PRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS104 / POLYETHER ANTIBIOTICSMONENSIN, NARASIN, & SALINOMYCINPolyether antibiotics, such as Monensin, Narasin, and Salinomycin, in raw material, premix, liquid supplements, andnal feeds, are best quantied using a high-performance liquid chromatograph equipped with a post-column reactor. Thepolyether antibiotics react, post-column, with Vanillin in the presence of Sulfuric acid, and the resulting products can bedetected at 520 nm. However, this corrosive reagent can adversely degrade most instruments. Pickering’s special Post-columnSystems, comprised of non-metallic parts and washing pump head, offers resistance to sulfuric acid. Special operatinginstructions will also be included with this instrument. Contact Pickering Laboratories for more information about the InertVector PCX Post-column System for polyether antibiotics.METHODAnalytical ConditionsColumn: Polyether reversed-phase column, C 18,4.6 x 250 mm, Catalog No. 2381750Temperature: 40 °CFlow Rate: 0.7 mL/minMobile Phase: Methanol/5 % acetic acid inwater (90/10), IsocraticPost-column ConditionsPost-column System: Vector PCX or Pinnacle PCX(Non-metallic post-column derivatization system is required)Reagent 1: Concentrated Sulfuric acid/Methanol (4:96 v/v)Reactor 1: Ambient, 0.1 mLReagent 2: 60 g of Vanillin (or p-dimethylaminobenzaldehyde)in 950 mL of MethanolReactor 2: 90 °C, 1.4 mLFlow Rate: 0.3 mL/minDetection: UV-Vis detector:Vanillin =520 nm, DMAB =450 nmMonensin B0 5 10 15 20 25Monensin ASalinomycinNarasinminREFERENCES1) M.R. LaPointe and H. Cohen, J.A.O.A.C., 71 (1988) 480–484.2) J.M. Rodewald, J.N. Moran, A.L. Donoho, and M.R. Coleman,J.A.O.A.C., 75 (1992) 272–279.

METHOD ABSTRACTS117 / PARAQUAT AND DIQUAT IN WATERDIRECT INJECTION METHODParaquat and Diquat are widely used in agriculture and industry. They are employed as contact herbicides, as harvest aids,for pasture renovation and for aquatic weed control. Their environmental persistence allows for contamination of watersupplies; wells, lakes, rivers, etc. Since Paraquat is a Class I Toxin and Diquat is a Class II Toxin, water resources must bemonitored for the presence of these herbicides.This method employs ion exchange chromatography so ltration is the only sample preparation necessary. Also, since bothParaquat and Diquat have a xed +2 charge, 2 mL can be injected without peak degradation. Post-column derivitizationimproves sensitivity and greatly improves selectivity by shifting the detection to longer wavelengths.METHODAnalytical ConditionsColumn: ALKION column 4 x 150mm,Catalog No. 9410917, Guard column 3 x 20mm,Catalog No. 9493020Temperature: 50 °CFlow Rate: 0.8 mL/minMobile Phase:1700-1101, Potassium buffer, K011700-1102, Potassium titrant, K021700-1103, Potassium ionic strength adjuster, K03Injection Volume: 2 mLPost-column ConditionsPost-column System: Pinnacle PCXReaction Dwell: 0.15 mLTemperature: 35 °CReagent: 300 mg/L Sodium Dithionite in0.3 M Sodium HydroxideFlow Rate: 0.3 mL/minDetection: 395 nm for Paraquat and 378 nm for DiquatWithout post-column derivatization:257 nm for Paraquat and 308 nm for DiquatDiquatParaquat0 2 4 6 8 10 12CONDITIONSTIME (Min) 1700-1101 % 1700-1102 % 1700-1103 % IPA %0 31.5 53.5 0 1510 28 47 10 1515 28 47 10 1515.1 0 85 10 517 0 85 10 517.1 31.5 53.5 0 15min125PRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS124 / SULFONAMIDE ARTIFICIAL SWEETENERS IN FOOD PRODUCTSANALYSIS OF ACESULFAME, SACCHARIN AND CYCLAMATE BY HPLC WITH POST-COLUMN DERIVATIZATIONNonnutritive sweeteners are widely used in foods and beverages. Since some studies raised questions about long-term safetyof these compounds, their concentration in food products is regulated. Among the articial sweeteners used worldwideSaccharin and Cyclamate cause most controversy and their use is restricted in many countries, including the US. Liquidchromatography is a method of choice for articial sweeteners but Cyclamate analysis is complicated by the fact that thiscompound does not exhibit noticeable UV absorbance.This method allows for simultaneous analysis of Acesulfame, Saccharin and Cyclamate by LC with post-column derivatizationfollowed by uorescence detection. Since the sweeteners are commonly used in combination, a single method ofanalysis is preferred.METHODAnalytical ConditionsAnalytical Column: Reversed-phase column, C18, 2x100 mmTemperature: 50 CFlow Rate: 0.2 mL/minMobile Phase:0.02 M Potassium Phosphate Monobasic in water. Isocraticfor 8 min, followed by washing the column with 70 % ACN– 30 % 0.02 M KH 2PO 4Post-column ConditionsPost-column System: Pinnacle PCXReagent: Hexadecyltrimethyl ammonium bromide (10-3 M),1,6 Diphenyl-1,3,5-hexatriene (4x10-6 M)Reactor: 40 C, 0.1 mLFlow Rate: 0.3 mL/minDetection: Fluorescence, ex= 365 nm, em= 460 nmAcesulfame KSaccharinCyclamate0 2.5 57.5 minChromatograms of a) Diet cola containing Acesulfame K; b) Soft drink spiked with 60 ppm ofartifi cial sweeteners.baREFERENCESJames F. Lawrence, Analyst, vol. 112, No 6 (1987), 879-881James F. Lawrence, Claudette F. Charbonneau, J. Assoc. Anal.Chem., vol. 71, No 5 (1988), 934-937

METHOD ABSTRACTS327 / ANALYSIS OF VOGLIBOSE IN PHARMACEUTICAL FORMULATIONSBY HPLC WITH POST-COLUMN DERIVATIZATIONVoglibose is an Alpha-Glucosidase inhibitor widely used for the treatment of diabetes. Alpha-glucosidase inhibitors areagents that delay the glucose absorption at the intestinal level and thereby prevent sudden surge of glucose after a meal.Vogilbose is the safest and most effective drug of its class.Since Vogibose has no UV chromophore, post-column derivatization is employed to produce a uorescent derivative.This abstract describes a very sensitive and robust analytical method for the analysis of Voglibose in pharmaceutical tablets.Simple sample preparation and fast analysis time allow for using this method in high throughput environments.METHODAnalytical ConditionsColumn: Amino column,4.6x250 mm,Catalog number 1446250Temperature: 35 °CFlow Rate: 0.6 mL/minMobile Phase: Sodium phosphate buffer, 20 mMpH 6.5 / Acetonitrile (37 : 63)Injection Volume: 50 µLSample PreparationCrush 5 tablets and mix with 25 mL of mobile phase.Sonicate for 10 min and lter liquid portion through0.45 µm lter. Put in HPLC autosampler vial and inject 50 µL.Post-column ConditionsPost-column System: Pinnacle PCXHeated Reactor Volume: 3.5 mLTemperature: 100 °CCooling Coil: 0.15 mL (at room temperature)Reagent: Taurine (6.25 g), Sodium Periodate (2.56 g)in 1000 mL of waterFlow Rate: 0.6 mL/minDetection: FLD ex: 350 nm, em: 430 nm127Repeatability studies fordifferent concentration levels.0.5 ppm 100 ppmAverage RT, min 21.25 21.26RSD, %, N = 6 0.36 0.08Average Peak Area 9.22 1,562.69RSD, %, N = 6 1.48 0.79Continued on next pagePRODUCT CATALOGPICKERING LABORATORIES10/11

METHOD ABSTRACTS327 continued from previous page35003000y = 16.468x + 4.7493R 2 = 0.99982500Peak Area20001500100050000 50 100 150 200 250Concentration, ppmCalibration Curve for Analytical Range 0.5-200 ppmVoglibose0 5 10 15 20 25Chromatogram of Voglibose Standard,50 ppm, 50 µL InjectionminVogliboseVoglibose0 5 10 15 20 25Chromatogram of Voglibose Standard,0.5 ppm, 50 uL Injectionmin0 5 10 15 20 25Chromatogram of Voglibose Tablets (Volix TM , 0.2 mg),50 µL Injectionmin

NOTES129PRODUCT CATALOGPICKERING LABORATORIES10/11

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THIS SECTIONSTATES SELLER’S TOTAL RESPONSIBILITY AND LIABILITY, AND THEBUYER’S SOLE REMEDY FOR ANY ACTUAL OR ALLEGED INFRINGE-MENT OF ANY PATENT, TRADEMARK, OR COPYRIGHT BY ANYGOODS DELIVERED HEREUNDER OR ANY PART THEREOF. THISSECTION 8 IS IN LIEU OF AND REPLACES ANY OTHER EXPRESS,IMPLIED OR STATUTORY WARRANTY AGAINST INFRINGEMENT.(b) The Buyer shall, at its own expense, indemnify and hold Seller harmless fromand against any expense or loss resulting from any infringement of any patent,trademark, or copyright arising as a result of Seller’s compliance with any ofthe Buyer’s designs, specications, or instructions, and shall defend at its ownexpense including attorney fees, any suit brought against Seller alleging any suchinfringements provided that Seller (i) gives the Buyer immediate notice of any suchsuit and permits the Buyer through counsel of its choice, to defend such suit, and(ii) gives Buyer all needed information, assistance, and authority, at the Buyer’sexpense, necessary for the Buyer to defend any such suit.9 ORDER CANCELLATIONBuyer may, at any time, cancel its purchase of goods or services but only by paying15 % of the purchase price if such cancellation is received by Seller more than 90days before the scheduled delivery date, 50 % if received more than 45 days but lessthan 90 days, 75 % if received more than 15 days but less than 45 days, and 100 % ifreceived less than 15 days.10 WARRANTY AND LIMITATION OF LIABILITYIN NO EVENT SHALL SELLER BE LIABLE FOR ANY INDIRECT, SPECIAL,INCIDENTAL, OR CONSEQUENTIAL DAMAGES RESULTING FROMSELLER’S PERFORMANCE OR FAILURE TO PERFORM HEREUNDER ORTHE FURNISHING, PERFORMANCE, OR USE OF ANY GOODS OR SER-VICES SOLD PURSUANT HERETO. IN NO EVENT SHALL THE AMOIJNTOF SELLER’S LIABILITY EXCEED THE AMOUNTS PAYABLE BY BUYERHEREUNDER. SELLER EXPRESSLY DISCLAIMS ANY LIABILITY FOR ANYWARRANTY, EXPRESS, IMPLIED OR STATUTORY, INCLUDED BUT NOTLIMITED TO WARRANTY OF MERCHANTABILITY AND FITNESS FOR APARTICULAR PURPOSE, EXCEPT SUCH WARRANTIES EXPRESSLY IDEN-TIFIED AS WARRANTIES AS ARE SET FORTH IN SELLER’S CURRENTOPERATING MANUAL, CATALOG OR OTHER WRITTEN GUARANTYCOVERING THE PRODUCT.11 GENERAL(a) The sale of goods shall be governed by Uniform Commercial Code and otherslaws of the State of California and Santa Clara County, California shall be theappropriate venue and jurisdiction for the resolution of disputes hereunder.(b) The Buyer may not assign its rights or obligations under this acknowledgementwithout the prior written consent of Seller, and any purported assignment withoutsuch consent shall have no force or effect.(c) Any waiver by Seller of any default by the Buyer hereunder shall not be deemed tobe a continuing waiver of such default or a waiver of any other default or any ofthe terms and conditions of this acknowledgement.(d) These T & C’s may not be superseded, modied, or amended except in writingstating that there is such a modication and signed by an authorized representativeof each party hereto, provided, however that Seller may modify the specicationsof the goods sold hereunder if such modication does not change the form, t, orfunction of such goods.(e) This document constitutes the entire terms and conditions agreement between theBuyer and Seller with regard to the goods or services purchased or provided andexpressly supersedes and replaces any prior or contemporaneous agreements, writtenor oral relating to such goods or services.(f) In no event shall Buyer commence any action under this contract later than oneyear after the cause of action has accrued.

DISTRIBUTORSArgentinaOmnilab srlVirrey Arredondo 2850Buenos Aires, 1426 ArgentinaTel: 54-11-4553-1100Fax: 54-11-4555-1584Email: info@omnilab.com.arAnalytical Technologies S.A.Avda. Cordoba 1131C1055AAB, Capital FederalBuenos Aires, ArgentinaTel: +54-11-4814-4445Fax: +54-11-4814-4445Email: info@analytical-tech.comAustraliaBiolab Ltd.2 Clayton RoadClayton, VIC 3168 AustraliaTel: 61-3-9263-4300Fax: 61-3-9562-7953Email: info@aus.biolabgroup.comWalker Scientifi cUnit 2, 5 Ledger RoadBalcatta, 6021 Western AustraliaTel: 61-8-9240-5255Fax: 61-8-9240-5266Email: gwalker@business.ibc.com.auA.I. Scientifi c Pty. Ltd.10-22 Hornibrook EsplanadeClontarf, Qld, 4019 AustraliaTel: 61-7-3105-5000Fax: 61-7-3283-7933Email: aimail@aiscientifi c.comWebsite: www.aiscientifi c.comBoliviaSistemas Analíticos SRLCalle Barachavi 138- Casilla de Correo2853-Santa Cruz de la Sierra, BoliviaTel: ++591 3 351 6298Fax: ++591 3 311 9209Email: cmontes@sistemasanaliticos.com.boWebsite: www.sistemasanaliticos.com.boBrasilCromatecRua Charles Darwin, 483Vila Sta CatarinaSao Paul - SP CEP. 04379-060Tel: 55-11-5670-2510Fax: 55-11-5670-2522Email: cromatec@cromatec.com.brWebsite: www.cromatec.com.brUNITECH Brasil (offices in DF, RJ, SP, RS)Av. Brasilia, 1080 – 2o. AndarPaulinia, Sao Paulo, Brasil 13140-000Tel: (19) 3833-4062Fax: (19) 3874-4841Website: www.UNITECHusa.comInterprise Instrumentos Analiticos Ltda.Rua Maria Das DoresLeal de Queiroz, 784Paulina, SP 131400-000 BrasilTel: 55-19-3874-7147Fax: 55-19-3874-7140Agilent TechnologiesAlameda Araguaia, 1142Alphaville BarueriSao Paulo, 06455-940 BrasilTel: 55-11-7297-3776Fax: 55-11-7297-3798Chemetric Instrumentacao Analitica LtdaEstr. Dos Bandeirantes 1430/201Jacarepagua, Rio de JaneiroCEP 22710-113 – RJ, BrasilTel: 55-21-2426-6236 / 6251Email: chemetric@chemetric.com.brWebsite: www.chemetric.com.brMerck S.A.,Estrada dos Bandeirantes, 1099Cep: 22710-571JacarepaguáRio de Janeiro - RJ - BrasilTel: +55 (21) 2444-2000Fax: 2445-2263Website: www.merck.com.brNOVA ANALÍTICAIMPORTAÇÃO E EXPORTAÇÃO LTDARua Assungui, 43204131-000 - São Paulo, SPTel: +55 11 2162 8080Fax: + 55 11 2162 8081Email: adriene.sandoval@novanalitica.com.brWebsite: www.analiticaweb.com.brChileEquilab Ltda.San Isidro 1839Santiago, ChileTel: 56-2-5569974Fax: 56-2-5514006Email: ventas2@equilab.clChina & Hong Kong TerritoriesLWL Development Ltd.Room 1920-21, Metro Centre II21 Lam Hing Street, Kowloon BayKoowloon, Hong KongTel: 852-27572332Fax: 852-27573811Email: lwlltlhk@netvigator.comTegent Technologies Ltd.Room 2103-05 Westin Centre26 Hung To Road, Kwun TongKowloon, Hong KongTel: 852-2759-2182Fax: 852-2758-3830Email: tegent@netvigator.comAgilent TechnologiesAsia Pacifi c Service CentreCSA for Life Science & Chemical Analysis GroupTel: 65-62157607Fax: 65-62157925Toll-free for China:Tel: 800-810-0712Fax: 800-810-0717Suntek Science Instruments Co., Ltd.Suite 501, No. 240 Baidi RoadTianjin, China 300192Tel: 022-8789-0415Fax: 022-8789-0417General Manager: Mr. Zhang, Bin-ChenAgilent Technologies Shanghai Branch12 F Novel Building, No. 887, HuaihaiZhong Road, Shanghai, 200020 ChinaTel: 86-21-64456247Fax: 86-21-64747708Agilent Technologies Hong Kong Branch24 F, Cityplaza One, 1111 Kings RoadTaikoo Shing, Hong KongTel: 852-652157609Fax: 852-652726037ColombiaUNITECH ColombiaCalle 38, #45-46Barranquilla, ColombiaTel: +57-5-340-8536Fax: +57-5-340-7536Website: www.UNITECHusa.comCasa Cientifi caCarrera 27A 49ª41Bogota, ColombiaTel: +571-3126310Fax: +571-3126304Email: casacam@casacientifi ca.comWebsite: www.casacientifi ca.comCosta RicaOrgoma S.A.El Carmen de Paso Anco175 Mts al Este, San Jose, Costa RicaTel: 506-226-0906Fax: 506-226-0408Email: orgoma@racsa.co.crElectronicaP.O. Box 2733-1000Hacienda Vieja CurridabatSan Jose, Costa RicaTel: 506-272-3700Fax: 506-272-0460Email: biocien@racsa.co.crUNITECH Costa Rica200 metros Norte Y 10 metros Oestedel Restaurante La Casona del PuebloSan Antonio de Coronado, Casa #123San Jose, Costa RicaTelefax: +506-294-0768Website: www.UNITECHusa.comEcuadorUNITECH EcuadorGuerrero Martinez 519 y P.P. GomezUrdaneta, Guayaquil, EcuadorTel: +593-4-236-0976Fax: +593-4-237-4435Website: www.UNITECHusa.comEuropean TerritoriesLCTech GmbHBahnweg 41D-84405 Dorfen, GermanyTel: 49(0)-80 81-93 68-0Fax: 49(0)-80 81-93 68-10Email: info@lctech.deGuatemalaAnalitica QuimicaP.O. 750, Km 15.5 CarreteraRoosevelt Zona 11, Centro ComercialMolino Local 22Guatemala Ciudad, GuatemalaTel: 502-2-435-5360Fax: 502-2-435-4464Email: anaqui@guate.netIPESA9 CALLE 15-45 ZONA 13, Edifi cio PlazaTrafalgarGuatemala, C. A. 01013Tel: +(502)384 8181Fax: +(502)384 8111Email: recepcion_daq@ipesa.comIndiaSpinco Biotech PVT LTDNo. 4 Vaidyaram StreetT. Nagar, Madras - 600 017 India+91 44 2434 01 74 phone+91 44 2434 07 61 FaxIndonesiaPT. Perkindo Mitra AnalitikaKomplek Graha Kencana Blok BHJI. Raya Pejuangan 88 KebonJeruk, Jakarta Barat, IndonesiaTel: 62-21-5332449Fax: 62-21-53673916Email: perkindo@cbn.net.idIsraelManbar Tech17A Lazarov StreetRishon Le-Zion, 75106 IsraelTel: 972-3-9512020Fax: 972-3-9511099KoreaYoung Lin Instrument Co. Ltd.899-6 Hogye 2-dong, Dongan-gu,Anyang-si, Kyonggi-do, 431-836 KoreaTel: 82-15443744Fax: 82-31-4673770Interface Engineering Co. Ltd.8F, BMS Building, 829, Yeoksam-dong,Gangnam-gu, Seoul, Korea, 135-936Tel: 82-2-4002605Fax: 82-2-4060762Email: ckjoo@interface.co.krIn Sung Chroma-Tech Co. Ltd.In Sung Bldg., 89-106, Shinjung 2-Dong,Yangcheon-Ku, Seoul, KoreaTel: 82-2-2644-1991Fax: 82-2-2644-1996MalaysiaFisher Scientifi c (M) Sdn Bhd.No. 3, Jalan Sepadu 25/123Taman Perindustrian Axis, Seksyen 2540400 Shah Alam, Darul EhsanSelangor, MalaysiaTel: 60-3-5228888Fax: 60-3-5218899MexicoABC Instrumentación Analítica S.A. de C. V,Tigre No.33 Col. Actipan del ValleC.P. 03230 México DF.Tel: 01 55 5524-2208Fax: 01 55 5534-4107Website: www.abcia.com.mxEmail: ventasvap@abcia.com.mxPerkin Elmer de Mexico S.A.Macedonio Alcal No. 54Col. Guadalupe InnMexico D.F., 01020 MexicoTel: 52-55-56517077Fax: 52-55-56607734Sintesis y Formulaciones deAlta Tecnologia SA de CVOlmo No. 32, Col. Valle de Pinos54040 TlainepantlaEdo. De Mexico, MexicoTel: 52-55-53975008Fax: 52-55-53976658Email: sifatec@webtelmex.net.mxVarian S.A.Concepcion Beistegui No. 109Col. Del Valle, 3100 MexicoTel: 52-55-5239162Fax: 52-55-5239472New ZealandAlphatech Systems & Co. Ltd.P. O. Box 62613Ellerslie South, 1131Auckland, New ZealandTelephone: ++64 9 580 1959Fax: ++64 9 580 2044Email: sales@alphatech.co.nzMedtec Products Ltd.Unit C, 8 Vega PlaceMairangi Bay, Aukland, New ZealandTel: 64-9-479-1068Fax: 64-9-479-1450Email: customer.service@medtec.co.nzPanamaPromed S.A.Urb. Industrial Costa del Este, Calle2daEdifi cio Promed, Apartado 6281Zona 5, Republic of PanamaTel: 507-271-3149Fax: 507-271-3164Email: segundo@promed.com.paPeruCientifi ca Andina S.A.C.Av. Dos De Mayo No. 276Mirafl ores, Lima 18, PeruTel: 51-1-444-9983Fax: 51-1-444-9937Sulabsa Suministros de Laboratorio S.A.Av. Javier Prado Este 1570, #301Lima 27, PeruTel: 51-1-224-5398Fax: 51-1-224-4418UNITECH PeruParque San Fernando, 141La Perla Alta, Callao, PeruTel: +51-1-420-3804Fax: +51-1-420-3660Website: www.UNITECHusa.comPhilippinesDAKILA TRADING CORPORATION208 Pilar StreetMandaluyong City, 1550 PhilippinesContact: Mr. Richard TeeTel: (632) 724-75-11Fax: (632) 721-07-39Email: sales@dakila.com131Continued on next pagePRODUCT CATALOGPICKERING LABORATORIES10/11

DISTRIBUTORSContinued from previous pageSingaporeAgilent TechnologiesAsia Pacifi c Service CentreCSA for Life Science &Chemical Analysis GroupTel: 65-62157607Fax: 65-62157925Agilent Technologies Singapore (Sales) Pte LtdLife Sciences and Chemical Analysis1 Yishun Avenue 7Singapore 768923Tel: +65-6377-1688 - Main line(ask for LSCA Sales)Fax: +65-6822-8247Perkin Elmer Asia29, International Business Park#03-04/06, Acer Bldg, Tower B609923 SingaporeTel: 65-8962136Fax: 65-8962146South AfricaAnatech Instruments13 Langwa StreetStrydompark Randburg, South AfricaTel: 27-11-7923300Fax: 27-11-7923363Email: sales@anatech.co.zaChemetrix Pty Ltd.Block J, Central Park, 400 16th RoadMidrand, South AfricaTel: 27-11-5419801Fax: 27-11-5419802TaiwanInpac International Corp.12F, No. 79 Sec. 1 Hsin Hai RoadTaipei, R.O.C. 106 TaiwanTel: 886-2-23637996Fax: 886-2-23923975Email: inpac@ms10.hinet.netVercotech Inc.6F No. 7-1 An Hsing Road, Hsin TienTaipeishien 231, R.O.C. TaiwanTel: 886-2-22113939Fax: 886-2-22115522Email: vercopak@ms5.hinet.netAnalab Corporation6F-2, No. 181 Sec. 2 Changan East RoadTaipei, R.O.C. TaiwanTel: 886-227-766931Fax: 886-227766932Email: analab@ms46.hinet.netAbetech Taiwan Inc.11F, No. 208 Rueiguang RoadTaipei, R.O.C. TaiwanTel: 886-2-87512323Fax: 886-2-887512020ThailandFortune Scientifi c Co, Ltd.301/34 M.6 Phaholyothin RoadAnusawaree, Bangkhen, Bangkok,10220 THAILANDTel: 662 986 1250 till 1254Fax: 662 986 1255, 662 986 1243Email: sales@fortunesci.comWebsite: www.fortunesci.comExecutive Trading Limited55/194-5 Moo 6, Soi Yothin Phattana, PraditManutham Road, LatphraoBangkok 10230 ThailandTel: 662-5150150Fax: 662-5150144Email: exec@ji-net.comPerkin Elmer Ltd.290 (1st fl oor), Raintree Offi ce GardenSoi Japanese School, Rama 9 RoadKhwang Bangkapi, Khet Huay KhwangBangkok, 10320 ThailandTel: 66-2-3197901Fax: 66-2-3197900UruguayPablo Ferrando S.A.P.O. Box 370, Avda Italia 2877Montevideo, 11600 UruguayTel: 598-2-4872586Fax: 598-2-4870131Email: ferrando@netgate.com.uyASMGabriel Pereira 2841 Apto. 60111300 Montevideo, UruguayTel / Fax: 598-2-709-6955Email: info@asm.com.uyUSAgilent TechnologiesLife Sciences/Chemical Analysis GroupTel: 800-227-9770 or 302-993-5304Fax: 302-633-5301Email: cag_sales-na@agilent.comWebsite: www.agilent.com4CHROM Inc.3015 Barrow DriveRaleigh, NC 27616Tel: 866-424-7661 or 919-872-5700Fax: 919-872-5738techsupport@4chrom.comChrom Tech, Inc.PO Box 240248Apple Valley, MN 55124 Tel: 800-822-5242or 952-431-6000Fax: 952-431-6345Email: sales@chromtech.comWebsite: www.chromtech.comFisher Scientifi c International Inc.Customer Service & Sales SupportTel: 800-766-7000Website: www.fi sherscientifi c.comGovernment Scientifi c Source, Inc.8460 Tyco RoadVienna, VA 22182Tel: 800-248-8030 or 703-734-1805Fax: 703-734-1803Email: sales@govsci.comWebsite: www.govsci.comP.J. Cobert Associates, Inc.PO Box 460046St. Louis, MO 63146Tel: 800-972-4766 or 314-993-2390Fax: 314-993-2491Email: cobert@cobertassoc.comWebsite: www.cobertassoc.comPerkin Elmer Corporation710 Bridgeport AvenueShelton, CT 06484-4794Tel: 800-762-4000 or 203-925-4600Fax: 203-925-4654Email: info@perkinelmer.comWebsite: www.perkinelmer.comPhanco P.R.PO Box 10464Granada Hill 1-APonce, PR 00732Tel: 787-844-7371Fax: 787-843-0657Email: phancopr@hotmail.comSci-Con631 Executive DriveWinter Park, FL 32789Tel: 800-433-6865 or 407-647-4900Fax: 407-644-4355Email: jimwscicon1@cs.comWebsite: www.scicon.netShimadzu Scientifi c Instruments7102 Riverwood DriveColumbia, MD 21046Tel: 800-477-1227Email: webmaster@shimadzu.comWebsite: www.shimadzu.comThompson Instruments354 Tyler Rd.Clear Brook, VA 22624Tel: 540-667-7044Fax: 540-667-6878Email: info@hplc.comThomson Instrument Company1121 South Cleveland StreetOceanside, CA 92054Tel: 800-541-4792 or 760-757-9182Fax: 760-757-9367Email: folks@hplc1.comWebsite: www.hplc1.comVWR International1310 Goshen ParkwayWest Chester, PA 19380Tel: 800-932-5000Email: solutions@vwr.comWebsite: www.vwr.comVenezuelaCorporacion Cientifi ca VenezolanaC.A. ComercialLibertador Piso 1 Ofc. 1-2Caracas, 1070 VenezuelaTel: 58-2-127614757Fax: 58-2-127623050Email: ccvjharb@telcel.net.veAgilent Technologies de VenezuelaLife Science & Chemical Analysis GroupTel: 58-2-122094609Fax: 58-2-122094618VietnamTramat Co., Ltd.12 Hang Duong StreetHanoi, VietnamContact: Phung Ngoc PhucEmail: phuc_phungngoc@tramat.com.vnTel: +84-4-8281631Fax: +84-4-9283289Offi ce email: info@tramat.com.vnWebsite: http://www.tramat.com.vnFor all other countries, please contactPickering Laboratories directly.

CATALYST FOR SUCCESSPickering Laboratories, Inc.1280 Space Park Way / Mountain View, CA 94043 / www.pickeringlabs.comsales@pickeringlabs.com / support@pickeringlabs.com800-654-3330 / 650-694-6700 / Fax: 650-968-0749