Herbal plants used in TCM as a source of compounds with ... - Bora

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AbstractEight compounds were obtained from an ethanol extract from the aerial parts of Abies forrestii,by use of several different chromatographic techniques (size exclusion, TLC, normal andreverse phase). The chemical structure was determined for three of them by use of MS- andNMR-techniques (the known diterpenes abietic acid, dehydroabietic acid, 9,13(β)-epidioxy-8(14)-abieten-18-oic acid) and for one of them by comparing to a standard on TLC (β-sitosterol).The rest was either not pure enough or was obtained in a too low concentration. The pulverizedplant material was extracted with ethanol, purified with ethylacetate and fractionated withgradient elution over a silica gel column and a MCI-column. The compounds were isolated andpurified over silica gel-, Sephadex LH-20- and ODS-columns, and with P-TLC. The structuresof the compounds were determined by a combination of LC-ESI MS, 1 H- and 13 C NMR data.The extracted compounds are well known compounds in other Abies species, but no recordshave been found describing earlier extraction from Abies forrestii.-4-
Table of ContentsPreface ......................................................................................................................................... 3Abstract ....................................................................................................................................... 41 Introduction .......................................................................................................................... 71.1 TCMCANCER project ..................................................................................................... 71.1.1 Modern Research Center of Traditional Chinese Medicine (MRCTCM), SecondMilitary Medical University (SMMU) ....................................................................................... 71.2 Traditional Chinese Medicine (TCM) .............................................................................. 81.2.1 Yin and Yang ........................................................................................................... 81.2.2 Qi ............................................................................................................................. 91.2.3 Fundamental Textures .......................................................................................... 101.2.4 The Organs in TCM ............................................................................................... 101.2.5 The cause of illness or the pattern of symptoms ................................................... 101.2.6 Chinese Herbal medicine ...................................................................................... 111.2.7 Chinese Herbal Medicine as a source of compounds for Modern Medicine ......... 141.3 Abies (Fir) ..................................................................................................................... 151.3.1 Abies forrestii (Forrest fir) ...................................................................................... 161.4 Natural Products ........................................................................................................... 161.4.1 Terpenes ............................................................................................................... 172 Materials ............................................................................................................................. 212.1 Chemicals ..................................................................................................................... 212.2 Apparatuses and equipment ......................................................................................... 213 Methods and procedures .................................................................................................. 233.1 Chromatography ........................................................................................................... 233.1.1 Normal phase adsorption chromatography ........................................................... 233.1.2 Reverse phase adsorption chromatography ......................................................... 253.1.3 Size exclusion chromatography ............................................................................ 273.1.4 Thin-Layer Chromatography (TLC) ....................................................................... 283.2 Analytical spectroscopic methods ................................................................................ 313.2.1 Nuclear Magnetic Resonance (NMR) spectroscopy.............................................. 313.2.2 MS (Mass Spectrometry) ....................................................................................... 33-5-
Page 1: Herbal plants used inTraditional Ch
Page 6 and 7: 3.3 Natural Products Library at MRC
Page 8 and 9: IntroductionMRCTCM has developed a
Page 10 and 11: Introduction1.2.3 Fundamental Textu
Page 12 and 13: IntroductionClassification by thera
Page 14: Introduction1.2.7 Chinese Herbal Me
Page 17 and 18: Introduction1.4.1 TerpenesTerpenes
Page 19: IntroductionFrom triterpenes, compo
Page 22 and 23: • Agilent LC/MSD Trap XCT mass sp
Page 24 and 25: Methods and proceduresIn normal pha
Page 26 and 27: Methods and proceduresFigure 3.2: P
Page 28 and 29: Methods and proceduresSephadex LH-2
Page 30 and 31: Methods and proceduresof compounds
Page 32 and 33: Methods and proceduresdifferent fre
Page 34 and 35: Methods and proceduresmeasured from
Page 36 and 37: Results and discussionFigure 4.1: E
Page 38 and 39: Results and discussion4.2.1 E2a - M
Page 40 and 41: Results and discussionwas given the
Page 42 and 43: Results and discussionP:E=7:3 gave
Page 44 and 45: Results and discussionwas 20 ml, we
Page 46 and 47: Results and discussion4.3 Structura
Page 48 and 49: Results and discussionFigure 4.5: 1
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Results and discussionWith the exce
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Results and discussionFigure 4.12:
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Results and discussion4.3.4 C1, C4,
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Conclusion5 ConclusionThe history a
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References6 References1. Liu, Z., e
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