Mussel Report - Friends of Big Walnut Creek

Figure #List of Figures1 Map of the Big Walnut Creek watershed from Hoover Dam to its mouth showingthe 21 sample locations, the canoed reaches (red) and where access was from bridges(blue). 302 Extant state significant species of mussels in Big Walnut Creek collected in 2012.A – Megalonaias nervosa (Ohio endangered), B – Ligumia recta (Ohio Threatened),and C – Alasmidonta marginata, D – Ptychobranchus fasciolaris, E – Truncillatruncata, and F – Lampsilis fasciola (top, female, bottom male) (all Ohio SpecialInterest). 313 Line graph showing the relationship between the number of extant and extirpatedindividuals collected from each collection site from Big Walnut Creek from belowHoover Reservoir (Site 1) to immediately above its mouth (Site 21). 324 Line graph showing the relationship between the number of extant and extirpatedspecies collected from each collection site from Big Walnut Creek from belowHoover Reservoir (Site 1) to immediately above its mouth (Site 21). 335 Average difference between the total community (H’-Total) and the extantcommunity (H’-Extant) for the mussel communites of Big Walnut Creek.The upstream sites are sites 1-7, the midstream sites are sites 8-14, and thedownstream sites are sites 15-21. a = no significant difference between theH’-Total and H’-Extant and b shows a significant difference between the twomeasures of community diversity (p < 0.05) 346 Relationship between H’ (Shannon-Weiner Diversity Index) for the totalcommunity (live, freshly dead, weathered and subfossil) compared to extant(live and freshly dead). 357 Relationship between Mussel-IBI scores and how similar the total communitywas to the extant community (Jaccard Coeficient of Similarity). 368 Relationship between Mussel-IBI and the % extant species of mussels. 379 Relationship between Mussel-IBI (this study) and Fish-IBI (OEPA, 2004). 3810 OEPA (2003) Figure 50 showing IBI and MIwb scores for Big Walnut Creek from1982 to 2000. 394

IntroductionThe distribution of the mussels of Big Walnut Creek is not well known. This is a bitsurprising given the location of the stream in central Ohio and its proximity to other knownmussel rich streams (such as Big Darby Creek, the Scioto River and the Olentangy River).Hoggarth (1997) found the mussel diversity to be excellent in the Lockbourne Road area andused this information to oppose discharge of untreated water into the creek by Big Walnut Sandand Gravel, Inc. (Hoggarth, 1997). He found evidence of 28 species of mussels in this reachincluding specimens of one federally listed endangered species, Epioblasma triquetra (snuffbox),two Ohio endangered species, Quadrula cylindrica (rabbitsfoot), and Megalonaias nervosa(washboard), one Ohio threatened species, Truncilla donaciformis (fawn foot), and seven speciescurrently listed as species of concern in the state, Anodonta suborbiculata (round floater),Alasmidonta marginata (elktoe), Lasmigona compressa (creek heelsplitter), Pleurobema sintoxia(round pigtoe), Ptychobranchus fasciolaris (kidneyshell), Truncilla truncata (deertoe), andLampsilis fasciola (wavy-rayed pocketbook).On Earth Day of 2010, Hoggarth presented a talk to the Friends of Big Walnut Creekentitled, “Big Walnut Creek: the Eastern Gem of the Scioto.” Some of the data presented at thattime included data collected by Ohio EPA (2003), which clearly showed the high diversity oforganisms found in the creek, especially beyond the impacts of Hoover Reservoir, and the factthat this diversity and water quality had either remained high since at least 1982. The creek isknown to have supported 40 species of mussels (Watters et al., 2009), 44 species of fish(Hoggarth et al., 2000, 2004: Ohio EPA, 2003), IBI scores from 34 immediately downstream ofHoover Dam to 48 near its mouth (OEPA data, see OEPA, 2003), and QHEI scores from 82.0-6

shell often faded to white). The living and freshly dead component of the collection wasdetermine to represent an extant population at the site (regardless of if weathered and subfossilspecimens were found) and if only weathered and subfossil specimens were found, then thiscomponent of the collected mussels was determined to represent an extirpated species for thesite.If a specimen was found alive, its length was measured and approximate age wasdetermined by the annular ring method. Once all measurements were made, the mussels foundin the timed searches were returned to the area from which they were found or if found duringquadrat sampling, within the same quadrat. Some live mussels; especially specimens of L.cardium (plain pocketbook) and L. fasciola (wavy-rayed pocketbook) were exhibitingreproductive behaviors while submerged in the substrate. These mussels were identified andcounted, but left undisturbed. Several dead mussels were found in situ within the substratethroughout the lower reaches of the stream. These were collected as described above. Waterchemistry was also sampled each day of this study. Observations made included temperature,turbidity, conductivity, pH, and oxygen saturation.AnalysisThe numeric data collected on live and dead mussels were collected so that we coulddetermine mussel diversity, abundance, and overall community structure throughout the studyarea. The Shannon-Wiener Diversity Index (H’) was used to determine diversity throughout thestream. Two indices of diversity were determined for each sample reach; the diversity of extantindividuals and the total diversity at the site represented by extant and extirpated individuals.9

This was done to determine if the extant diversity was similar to the total diversity of mussels ateach site.The Jaccard Coefficient of Similarity and % extant species also were calculated todetermine if the two communities (extant and total) were similar or not. Jaccard Coefficient ofSimilarity examines the degree to which two groups of species are similar and % extant speciesexamines how many of the historic taxa at a site are still extant at that site. A Student T-testswas used to determine any significant difference in mussel community from site to site. Inaddition, a Mussel-IBI (Hoggarth and Goodman, in review) was calculated and compared to H’for the entire community, H’ for the extant community, Jaccard Coefficient of Similarity and %extant individuals for each site. The Mussel-IBI also was compared to Fish IBI and invertebratecommunity structure (ICI), whice were determined from collections made in 2000 by the OhioEPA (OEPA, 2003). Regression analysis was used to determine where a relationship betweenthese community parameters was significant. The following formulas were used to determinethese values:Shannon-Wiener Diversity Index: H’ = - Σ (p i lnp i )/N, where p i is the proportion of thetotal sample belonging to the i th species, and N is the total number of individualsJaccard Coefficient of Similarity: J = A and B/ A + b + c, where A and B are the numberof species present in both communities, b is the number of species present only in onecommunity and c is the number of species present only in the other community.Mussel-IBI: The mussels IBI is calculated as the Σ of metrics 1-10. Appendix 1 providesall of the data collected during this study and Appendix 2 provides a summary of Mussel-IBIcalculations for all sites with greater than three species of mussels.10

ResultsMussel DiversityDuring the current study, 31 species of mussels were found alive or as dead shells(freshly dead, weathered dead and subfossil shells). Of this number, 24 species were found to beextant (either collected alive or as freshly dead shells) and seven were only found as weatheredor subfossil shells (Table 3). Of the 24 species found to be extant, one (M. nervosa, washboard)is an Ohio Endangered Species and another (L. recta, black sandshell) is listed as Threatened inthe state. Other state significant species found alive during this study (all Ohio Species ofConcern) were A. marginata (elktoe), P. fasciolaris (kidneyshell), T. truncata (deertoe), and L.fasciola (wavy-rayed pocketbook) (Figure 2 contains representative photographs of thesespecies).In addition to the six species listed above, four Federal (including candidate species) andOhio Endangered Species (Q. clyindrica, rabbitsfoot, P clava, clubshell, E. triquetra, snuffbox,and E. t. rangiana, northern riffleshell), one additional Ohio Threatened Species (T.donaciformis, fawn foot), and one Ohio Species of Concern (P. sintoxia, round pigtoe) werefound, but only as extirpated specimens.Four species comprised more than 10% each of the entire fauna (extant and extirpated)and were widely distributed throughout the reach sampled during this study (Table 3). The mostabundant extant species by % of total were Elliptio dilatata (spike) (16.7%), Potamilus alatus(pink heelsplitter) (15.9%), Leptodea fragilis (fragile papershell) (14.8%) and Lampsilis radiataluteola (fat mucket) (10.9%). Elliptio dilatata (spike) was most commonly found in the upperreaches of the survey area whereas P. alatus (pink heelsplitter) and L. fragilis (fragile papershell)11

were more commonly found in the middle to lower reaches. Elliptio dilatata (spike), Amblemaplicata (threeridge), and Fusconia flava (Wabash pigtoe) were the most abundantly foundextirpated species with E. dilatata (spike) being found predominantly in the upper to middlereaches, A. plicata (threeridge) in the middle to lower reaches, and F. flava (Wabash pigtoe) inthe upper reaches.The number of extant individuals outnumbered the extirpated individuals at sites 1-8, 12-17, and 19-20 (Figure 3). The number of extirpated individuals was only greater at sites 9-11,18, and 21. In the upper reaches (sites 1-7), more extant species were found than extirpatedspecies except at site 2. In the middle reaches (sites 8-14), extant species outnumbered theextirpated species found except at sites 10 and 11. In the lower reaches (sites 15-21), the numberof extirpated species was greater than the number of extant species at every site except site 15(Figure 4). The mussel communities from Hoover Dam to Whitehall (RM 22.0) changed verylittle (Jaccard Coefficient = 83% and percent extant = 78%). H’ values for this reach were notsignificantly different when comparing the total and extant communities (t = 1.08, p > 0.05).However, the communities from RM 22.0 to RM 15.0 (just downstream of Three CreeksMetroPark) had fewer extant species (Jaccard Coefficient and percent extant values of 62% and36%) and significantly diminished species diversity (t = 2.35, p < 0.05). Diminished speciesdiversity continued to be expressed downstream (t = 2.48, p < 0.05), with some recovery(Jaccard Coefficient value of 67% and percent extant value of 42%) found in the lower reachesof the creek. We believe some of this recovery in the extreme lower reaches of Big WalnutCreek may be the result of movement of mussels from the Scioto River (Figure 5). In addition,although the relationship between H’-Extant and H’-Total was not significant (Figure 6), it doesdemonstrate that where the number of extant species are highest (as in the best sites, including12

some in the lower reaches of the creek), the two follow the regression line almost exactly. Thisfinding suggests a great deal of potential recovery for the mid-section of the creek where thesame cannot be said.Water Quality AssessmentThe Mussel-IBI is a good indicator of high quality mussel habitat and water quality(Tables 4 & 5 and Figures 7 & 8), but less so of symbiotic function (at least Fish-IBI scores)(Figure 9). Where the extant and total communities were more similar to each other (JaccardCoefficient had the highest values), Mussel-IBI scores also were highest (Figure 7). Similarly,where the largest numbers of extant species were found, the Mussel-IBI also had its highestvalues (Figure 8). Table 4 shows that the “Good” values for the Mussel-IBI were mostlyencountered in the upper reaches of the study area (sites 3, 4, 5 & 8), with the most “Poor” sitesfound in the mid-section of the study area, and then with mostly “Fair” to “Good” Mussel-IBIscores found downstream.It is interesting that the same was not true for the Fish-IBI scores calculated from 2000data by Ohio EPA (OEPA, 2003). It may be because of the time elapsed since the two studies(2000 – 2012) or the differences in what the mussel communities and fish communities can tellus about their environment (mussels leave behind a record in the form of a dead shell, which canbe used to assess the long-term suitability of a reach for aquatic animals), but it is apparent thatthe reaches with the best water resource integrity based on Fish-IBI (mid to lower sections of theriver) are not the same reaches where mussels suggest water resource integrity is highest (upperportion of the study area downstream of Hoover Reservoir). Figure 10 shows the Ohio EPAFish-IBI and MIwB data graphically and it clearly shows lower IBI scores where we found thehighest Mussel-IBI scores, the fewest extirpated species, and in many cases, the most number of13

complete their life cycle. Given these facts, they are excellent indicators of long-term waterresource integrity (Hoggarth and Goodman, in review).The fact that mussels tell us more about the long-term quality of the aquatic resourcesrather than the more immediate integrity of the resource can produce some difficulty ininterpreting the data. For example, we noticed many very old (perhaps 20-35 years old) musselsdead in situ in the lower portion of the creek (especially at sites 16 and 17: off of LockbourneRoad and upstream of the Rowe Road Bridge). The substrate around these shells had beenmostly washed away. These observations suggest these mussels were killed almost immediately(they did not try to move from the reach) and that they had been dead for a very long time. Itsuggests that some unknown water quality issue killed these mussels (old age is not suspectedsince there were so many shells in exactly the same position in the stream), but it is difficult tosay when this occurred other than to say it was some years ago. The fact that these same twosites produced relatively large numbers of living mussels, including rare species, suggests thesame water quality issues are not being experienced today. However, it is interesting that manyof the species that were eliminated (mostly species that bury in the substrate) are still gone. Thiscould be due to some latent problem associated with the substrate or it could be access, as manyof these species (such as Lasmigona costata, fluted-shell) are fairly rare to absent in much of thelower portion of the creek. Still, some analysis and interpretation of the mussel data we collectedis in order.The Upper Section (from Hoover Dam to Whitehall; sites 1-7)As noted previously, this section of the creek suffered the least loss of species and hadthe highest diversity and overall range of Mussel-IBI scores (all fair to good). Sites 1-4 are15

located upstream of the lowhead dam in Gahanna, while the remaining four sites are locatedfrom Gahanna to Whitehall. Site 3 (at Woodside Green Park) and Site 4 (adjacent to theGahanna Municipal Golf Course) produced an impressive number of live and freshly deadmussels. Only two other sites, Site 8 (at Big Walnut Park) and Site 16 (off of Lockbourne Road)produced similar numbers of live mussels.The best sites in this section (Sites 3 & 4) had significant riparian corridors and weremore or less protected from further development by being adjacent to a park and a golf course.The creek in these two areas had a natural channel without evidence of excessive nutrient and/orother pollutants entering the stream. Most of the live mussels found in these two reaches werecomplete buried in the substrate and fairly densely packed (Site 3 had an average density of 10mussels/m 2 and Site 4 had an average density of 4 mussels/m 2 ). For comparison, Site 8 had anaverage density of 6 mussels/m 2 and Site 16 had an average density of 0 mussels/m 2 from thequadrat sampling, but produced many more live mussels during the timed searches.The recovery potential for the mussel community in this reach varies depending on wherethe mussels are currently found. The recovery potential of Sites 1 – 4 are reduced by theirlocation between dams (Hoover Dam) and the lowhead dam in Gahanna. It seems unlikely thatfish with glochidia (the parasitic larvae of mussels) will be able to move upstream of the lowheaddam, and so recruitment of new species from downstream will be limited, or that fish withglochidia will emerge from outflow of water from Hoover Reservoir, since the reservoir does notsupport a riverine fauna of mussels. The section of the creek upstream of Site 3 could benefitfrom immigration; however, this too is limited by the presence of the lowhead dam at the HapCremean Water Plant. The recovery potential of Sites 5 – 7, however is enhanced by thepresence of Site 8 (Big Walnut Park), which produced a large number of live mussels.16

The Mid-Section (from Whitehall to State Route 317; sites 8-14)Other than Site 8 (Big Walnut Park), this reach of stream suffered the highest degree ofmussel loss examined during this study. This was surprising as this reach had the highest Fish-IBI scores and ICI scores of any of the stations examined by Ohio EPA in 2000 (OEPA, 2003).As noted above, Site 8 produced a diverse mussel community (an average density of 6mussels/m 2 ) with a good number of species that bury in the substrate (generally an indication ofoverall good, long-term habitat and water quality). As at Sites 3 & 4, the creek at this site wasfairly protected with a good riparian corridor and the substrate in the creek was stable andcomposed of sediments ideal for mussels (sand and gravel). There was no indication ofexcessive nutrients and/or other pollutants in the water (no excessive algae growth, for example).These same conditions were found just upstream and downstream of Three Creeks MetroPark (aswell as within the park itself), but very few mussels were found at the two sites on either side ofthe metropark (Sites 11 & 12) or within the metropark (no station was established in themetropark as no area of mussel concentration was found). Otherwise, this reach was dominatedby narrow riparian corridors and some reaches where indication of excessive nutrient loading(excessive algae covering the substrate) was apparent. Ohio EPA (OEPA, 2003) identifies somewastewater treatment issues associated with Alum and Blacklick creeks, which may be, or have,influenced water quality in Big Walnut Creek in and below Three Rivers MetroPark, but thereare probably more localized, point and non-point sources in this section of the river (impossibleto detect from the current study) that have affected water and/or habitat quality in this reach. Themussels are still reacting negatively to these disturbances, but do have the potential to improvefrom immigration of individuals from Site 8 and from the lower section of the river. No dams inthis portion of the creek would impair movement of mussels here.17

The Lower Section (from State Route 317 to the mouth; sites 15-21)Whatever has affected the mid-section of Big Walnut Creek also has to have affected thelower section of the creek. In addition, there are potentially other developments in the lowersections (such as sand and gravel operations; Hoggarth, 1997, OEPA, 2003) that would add tothe problems for mussels in this reach. Still, this reach appears to have experienced limitedrecovery. For example, even though L. recta (black sandshell) has historically been a member ofthe mussel fauna of Big Walnut Creek (Watters, et al., 2009), it is even more abundant andwidely distributed today. It is also one of the mussel species that has experienced significantrecovery in the Scioto River (Hoggarth, 2004, 2008, 2009b). In addition, the relatively largenumber or specimens of P. alatus (pink heelsplitter), P. ohiensis (pink papershell), L. fragilis(fragile papershell), and T. truncata (deertoe) suggest a viable connection with the Scioto River,where these species comprise significant components of the mussel fauna of that stream(Hoggarth, 2004, 2008, 2009a, 2009b). Other species that have always been common in BigWalnut Creek, such as Tritogonia verrucosa (pistolgrip) and Quadrula quadrula (mapleleaf) alsoare common today in the Scioto River, which could be a result of exchange from Big WalnutCreek to the Scioto River. It is not too long ago that this portion of the Scioto was devoid ofmussels (Personal Communication from Dave Stansbery) but today supports not only mussels,but fish and other invertebrates (OEPA, 2012). It is, perhaps, a bit surprising that Truncilladonaciformis (fawnfoot) has not moved into the lower section of Big Walnut Creek, as thisspecies uses the freshwater drum as host just as many of the other species that have moved intothe stream, but given its relatively small size it may have been missed during this study or it mayhave substrate and/or water quality requirements that are more selective than some of these other18

species. It may be a good indicator of improving habitat quality and water quality in Big WalnutCreek as we go forward.Of special note in the lower section of the creek is the continued presence of M. nervosa(washboard) in this reach. One live specimens of this Ohio endangered species was found at Site16 (off of Lockbourne Road). That species was found alive at this site by Hoggarth (1997) andgiven that the specimen found during the current survey was 35 years old (annular ring methodof determining age), it could easily have been one of the mussels found by Hoggarth more than16 years ago. This species is on the brink of extirpation in the creek and should be protectedfrom further decline. It has not been found recently in the Scioto River near the mouth of BigWalnut Creek (Hoggarth, 2004, 2008, 2009a, 2009b) and it is only rarely found in lower BigDarby Creek (Watters et al., 2009). This population, if it is viable, may be the bestrepresentation of this species in central Ohio, and could produce specimens that ultimately couldpopulate the Scioto River and Big Darby Creek. Its potential to affect the diversity of mussels isOhio is quite large.AcknowledgementsWe would like to thank the Friends of Big Walnut Creek for funding the study andOtterbein University Office of Sponsored Programs for managing the project. Their support ofthis project made this study possible as well as the report of our findings to the Ohio Academy ofScience as summarized in our published abstract in the Ohio Journal of Science (Appendix 3).Literature CitedHoggarth, M.A. 1997. Letter to Ohio EPA concerning a permit to discharge water in BigWalnut Creek by S&S Aggregate, 24 August 1997 (see Appendix 1).19

Hoggarth, M.A. 2004. Mussel survey and relocation on the Scioto River for PIC-22-17.03.HzW Environmental Consultants, Inc. 6105 Heisley Road, Mentor, Ohio 44060Hoggarth, M.A. 2006. Report on a Mussel Survey and Relocation for UNI 42-1.49 (PID 13500),Big Darby Creek. TranSystems Corporation, 55 Public Square, Suite 1650, Cleveland,Ohio 44113.Hoggarth, M.A. 2007. Report on the status of mussels relocated from Big Darby CreekFor UNI-42-1.49 (PID 13500) one year following relocation, Union County, Ohio.Final Report to ENTRAN, Cincinnati. Ohio. 13 pp.Hoggarth, M.A. 2008a. Report on the status of mussels relocated from Big Darby Creekfor UNI-42-1.49 (PID 13500) two years following relocation, Union County,Ohio. The Mannik & Smith Group, Inc., 1800 Indian Wood Circle, Maumee,Ohio 43537. 16 pages.Hoggarth, M.A. 2008b. Mussel Survey and Relocation for FRA 270-52.16 (PID 25733)Over the Scioto River, Columbus, Ohio. Final Report for ENTRAN, 1848Summit Road, Cincinnati, Ohio 45237. 30 pages.Hoggarth, M.A. 2009a. Report on the status of mussels relocated from the Scioto River atPIC CR 4- 2.64 (PID 77235) one year after relocation. Final Report for StrategicEnvironmental and Ecological Services, Inc., 653 McCorkle Boulevard, Suite G,Westerville, Ohio 43082. 11 pages.Hoggarth, M.A. 2009b. Report on the status of mussels relocated from the Scioto RiverSoutherly Wastewater Treatment Plan Effluent Conduit Outlet Channel. FinalReport for Stantec, 1500 Lake Shore Drive, Suite 100, Columbus, Ohio 43204. 11pages.Hoggarth, M.A. 2012. A report on a mussel survey of Big Walnut Creek at the InterstateRoute 270 Bridge in Franklin County, Ohio. Final Report to AmericanStructurepoint, Inc., 2550 Corporate Exchange, Suite 300, Columbus, Ohio43231. 18 pages.Hoggarth, M.A., B.A. Carr, C.R. Chandler, D.L. Konfal, B.L. Plummer, W.D. Rossiter,R.S. Smith, M.A. Stalnaker, and K.M Wittman. 2000. An Ecological Assessmentof Big Walnut Creek and its Tributaries in Gahanna. Final Report to the City ofGahanna (Ecology class project). Otterbein University, Department of Biologyand Earth Science.Hoggarth, M.A., R. Bears, D. Kimberly, P. La Susa, A. Lammers, C. Stull, K. Tilley, B.Van Allen, and J. Whitehall. 2004. An Ecological Assessment of Big WalnutCreek from Hoover Reservoir Dam to the City of Gahanna, Franklin County, Ohio. FinalReport to the City of Gahanna (Ecology class project). Otterbein University, Departmentof Biology and Earth Science.20

Hoggarth, M.A. and M.H. Goodman. In preparation. The development and application ofa Mussel Index of Biotic Integrity (Mussel-IBI) for the Little Miami RiverSystem, Ohio. Ecological Applications.Ohio EPA. 2003. Biological and Water Quality Study of the Big Walnut Creek Basin2000. Ohio EPA Technical Report DSW/EAS 2003-11-10.Ohio EPA. 2012. Biological and Water Quality Study of the Middle Scioto River and SelectTributaries, 2010: Delaware, Franklin, Pickaway, and Union Counties. Ohio EPATechnical Report DSW/EAS 2012-12-12.Watters, G. Thomas., Michael A. Hoggarth, and David H. Stansbery. 2009. The FreshwaterMussels of Ohio. The Ohio State Univeristy Press, Columbus, Ohio. 421 pages.21

Table 1. List of mussels recorded from the Big Walnut Creek watershed in Franklin andPickaway counties, Ohio by Watters, et al. 2009.Species Name Common Name Watershed BWC1. Utterbackia imbecillis paper pondshell Extant Extant2. Anodonta suborbiculata d round floater Extant Extant3. Pyganodon grandis giant floater Extant Extant4. Anodontoides ferussacianus cylindrical papershell Extant Extant5. Strophitus undulatus creeper Extant Extant6. Alasmidonta viridis slippershell Extant7. Alasmidonta marginata d elktoe Extant Extant8. Lasmigona costata fluted shell Extant Extant9. Lasmigona complanata white heelsplitter Extant Extant10. Lasmigona compressa d creek heelsplitter Extant Extant11. Simpsonaias ambigua salamander mussel Extant12. Tritogonia verrucosa pistolgrip Extant Extant13. Quadrula cylindrica a rabbitsfoot Extant Extant14. Quadrula quadrula mapleleaf Extant Extant15. Quadrula pustulosa pimpleback Extant Extant16. Amblema plicata threeridge Extant Extant17. Cyclonaias tuberculata purple pimpleback Extirpated Extirpated18. Fusconia flava Wabash pigtoe Extant Extant19. Pleurobema sintoxia d round pigtoe Extant Extant20. Pleurobema clava a clubshell Extant Extirpated21. Elliptio dilatata spike Extant Extant22. Elliptio crassidens b elephant ear Extant Extant23. Megalonaias nervosa b washboard Extirpated Extirpated24. Ptychobranchus fasciolaris d kidneyshell Extant Extant25. Actinonaias ligamentina carinata mucket Extirpated Extirpated26. Leptodea fragilis fragile papershell Extant Extant27. Potamilus alatus pink heelsplitter Extant Extant28. Potamilus ohiensis pink papershell Extirpated Extirpated29. Truncilla donaciformis c fawnsfoot Extant Extant30. Truncilla truncata c deertoe Extant Extant31. Obovaria subrotunda hickorynut Extant Extant32. Toxolasma parvum lilliput Extirpated Extirpated33. Ligumia recta c black sandshell Extirpated Extirpated34. Villosa fabalis a bean shell Extirpated Extirpated35. Villosa iris rainbow Extant Extant36. Lampsilis radiata luteola fatmucket Extant Extant37. Lampsilis cardium plain pocketbook Extant Extant22

Table 1. List of mussels recorded from the Big Walnut Creek watershed in Franklin andPickaway counties, Ohio by Watters et al. 2009. – continued –Species Name Common Name Watershed BWC38. Lampsilis fasciola d wavy-rayed pocketbook Extant Extant39. Epioblasma triquetra a snuffbox Extant Extant40. Epioblasma torulosa rangiana a northern riffleshell Extirpated ExtirpatedWatershed – species that has been collected from anywhere in the watershedBWC – species that has been collected from Big Walnut Creek in Franklin and/or PickawaycountiesExtant – collected alive or as a freshly dead shell; Extirpated – collected only as a weathered orsubfossil shell from the creeka – Federal and Ohio Endangered Species, b – Ohio Endangered Species, c – Ohio ThreatenedSpecies, d – Ohio Specie of Concern23

Table 2. Sample locations for a mussel study of lower Big Walnut Creek.Site #: location (00 o 00’00.00” = onsite GPS location; 00.000000 o = Google Earth GPS location),date sampled.Site 1: Big Walnut Creek downstream of Central College Road, at 40 o 05’54.5”N, 82 o 52’50.5”W,40.097607 o N -82.880414 o W, Franklin Co., Ohio, on 1 September 2012 and 2 September 2012.Site 2: Big Walnut Creek downstream of Morse Road, at 40 o 03’40.1”N, 82 o 53’27.3”W,40.051029 o N -82.890739 o W, Franklin Co., Ohio, on 9 September 2012.Site 3: Big Walnut Creek at Woodside Green Park, at 40 o 02’38.7”N, 82 o 52’47.9”W,40.044091 o N -82.879978 o W, Franklin Co., Ohio, on 22 September 2012.Site 4: Big Walnut Creek adjacent to Gahanna Municipal Golf Course, at 40 o 01’47.3”N,-82 o 52’40.2”W, 40.029562 o N 82.877703 o W, Franklin Co., Ohio, on 29 September 2012.Site 5: Big Walnut Creek at the IR 270 Bridge, at 40 o 01’47.3”N, 82 o 52’40.2”W, 40.006664 o N-82.874567 o W, Franklin Co., Ohio, on 29 September 2012.Site 6: Big Walnut Creek at Airport Golf Course downstream of Pizzuro Park, at 40 o 01’47.3”N,82 o 52’40.2”W, 39.993509 o N -82.857756 o W, Franklin Co., Ohio, on 29 September 2012.Site 7: Big Walnut Creek at Whitehall Community Center, at 39 o 58’54.5”N, 82 o 51’50.9”W,39.981531 o N -82.863957 o W, Franklin Co., Ohio, on 30 September 2012.Site 8: Big Walnut Creek at Big Walnut Park, at 39 o 56’57.0”N, 82 o 51’16.3”W, 39.949177 o N-82.854546 o W, Franklin Co., Ohio, on 30 September 2012.Site 9: Big Walnut Creek at Nerfzgel Park, at 39 o 54’47.3”N, 82 o 52’14.0”W, 39.912873 o N-82.870410 o W, Franklin Co., Ohio, on 4 October 2012.Site 10: Big Walnut Creek downstream of Hamilton Road (SR 317), at 39 o 54’36.4”N,82 o 52’57.4”W, 39.910023 o N -82.883249 o W, Franklin Co., Ohio, on 4 October 2012.Site 11: Big Walnut Creek at East Williams Road, at 39 o 53’15.6”N, 82 o 54’18.4”W, 39.887874 o N-82.905006 o W, Franklin Co., Ohio, on 11 October 2012.Site 12: Big Walnut Creek downstream of three rivers MetroPark, at 39 o 52’24.5”N,82 o 54’43.6”W, 39.873349 o N -82.912382 o W, Franklin Co., Ohio, on 11 October 2012.Site 13: Big Walnut Creek upstream of London-Groveport Bridge (SR 17), at 39 o 50’00.5”N,82 o 59’30.2”W, 39.833993 o N -82.980494 o W, Franklin Co., Ohio, on 13 September 2012.24

Table 2. Sample locations for a mussel study of lower Big Walnut Creek. – Continued –Site #: location (00 o 00’00.00” = onsite GPS location; 00.000000 o = Google Earth GPS location),date sampled.Site 14: Big Walnut Creek downstream of London-Groveport Bridge (SR 317), at 39 o 49’52.9”N,82 o 59’33.6”W, on 39.831382 o N -82.992654 o W, Franklin Co., Ohio, 13 September 2012.Site 15: Big Walnut Creek adjacent to gravel quarry, at 39 o 49’25.3”N, 82 o 59’23.1”W,39.823557 o N -82.990004 o W, Franklin Co., Ohio, on 15 September 2012.Site 16: Big Walnut Creek off of Lockbourne Road, at 39 o 58’11.3”N, 82 o 58’11.3”W,39.819118 o N -82.970161 o W, Franklin Co., Ohio, on 16 September 2012.Site 17: Big Walnut Creek upstream of Rowe Road Bridge (CR 133), at 39 o 48’42.8”N,82 o 58’11.3”W, 39.812071 o N -82.969446 o W, Franklin Co., Ohio, on 16 September 2012.Site 18: Big Walnut Creek downstream of Rowe Road Bridge (CR 133), at 39 o 48’34.6”N,82 o 58’38.8”W, 39.809502 o N -82.977974 o W, Franklin Co., Ohio, on 13 October 2012.Site 19: Big Walnut Creek downstream of US Rt. 23 Bridge, at 39 o 47’59.5”N, 83 o 00’14.9”W,39.8806785 o N -82.996485 o W, Franklin Co., Ohio, on 20 September 2012.Site 20: Big Walnut Creek further downstream of US Rt. 23 Bridge, at 39 o 47’59.5”N,83 o 00’14.9”W, 39.80012 o N -83.004117 o W, Pickaway Co., Ohio, on 20 September 2012.Site 21: Big Walnut Creek just upstream of its mouth, at 39 o 47’57.6”N, 83 o 00’34.4”W,39.799587 o N -83.007799 o W, Pickaway Co., Ohio, on 20 September 2012.25

Table 3. Extant (live and freshly dead collected) and extirpated (weathered and subfossilcollected) mussels collected from Big Walnut Creek in Franklin and Pickaway counties, Ohio in2012.Species Common Name Extant Extirpated# %1. Pyganodon grandis giant floater 12 1.7 22. Strophitus undulatus creeper 5 0.7 23. Alasmidonta marginata d elktoe 4 0.6 14. Lasmigona costata fluted shell 13 1.9 145. Lasmigona complanata white heelsplitter 2 0.3 106. Tritogonia verrucosa pistolgrip 29 4.2 97. Quadrula cylindrica a rabbitsfoot -- 18. Quadrula quadrula mapleleaf 4 0.6 119. Quadrula pustulosa pimpleback 2 0.3 810. Amblema plicata threeridge 17 2.5 2811. Cyclonaias tuberculata purple pimpleback -- 412. Fusconia flava Wabash pigtoe 51 7.4 2413. Pleurobema sintoxia d round pigtoe -- 514. Pleurobema clava a clubshell -- 115. Elliptio dilatata spike 115 16.7 3916. Megalonaias nervosa a washboard 2 0.3 317. Ptychobranchus fasciolaris d kidneyshell 47 6.8 1818. Leptodea fragilis fragile papershell 102 14.8 1019. Potamilus alatus pink heelsplitter 110 15.9 1120. Potamilus ohiensis pink papershell 2 0.3 --21. Truncilla donaciformis c fawnsfoot -- 222. Truncilla truncata c deertoe 7 1.0 623. Obovaria subrotunda hickorynut 3 0.4 624. Toxolasma parvum lilliput 1 0.1 --25. Ligumia recta c black sandshell 5 0.7 126. Villosa iris rainbow 13 1.9 --27. Lampsilis radiata luteola fatmucket 75 10.9 1628. Lampsilis cardium plain pocketbook 49 7.1 729. Lampsilis fasciola d wavy-rayed pocketbook 20 2.9 230. Epioblasma triquetra a snuffbox -- 531. Epioblasma torulosa rangiana northern riffleshell -- 1Total number of species 24 28Total number of individuals 690 247a - Federal & Ohio Endangered Species, b - Ohio Endangered Species, c - Ohio Threatened Species, d -Ohio Specie of Concern26

Table 4. Shannon-Weiner Diversity Index, Jaccard Coefficient of Similarity values, percentextant species values, and Mussel-IBI scores and values for the mussels collected from BigWalnut Creek in Franklin and Pickaway counties, Ohio in 2012.Site # H’Extant H’ Total Jaccard a % Extant Mussel-IBI% Similarity Score Value1 0.6642 0.6761 89 88 28 Fair2 0.5353 0.7495 69 55 24 Fair3 0.7310 0.7593 86 83 34 Good4 0.8975 0.9849 79 73 36 Good5 0.9089 0.9608 87 85 32 Good6 0.7068 0.8694 77 70 28 Fair7 0.5936 0.7088 78 63 22 Fair8 1.0369 1.0428 100 100 36 Good9 0.7782 0.9666 67 50 26 Fair10 0.6021 1.0066 61 36 16 Poor11 0.3768 1.0488 57 23 16 Poor12 0.4582 0.4582 -- b 100 -- b ----- b13 0.5448 0.6282 82 78 34 Good14 0.5579 0.7549 75 67 28 Fair15 0.4403 0.5676 -- b 67 -- b ----- b16 0.4326 1.1468 68 52 32 Good17 0.8537 1.0487 70 57 24 Fair18 0.0000 0.3510 -- b 0 -- b ----- b19 0.5951 0.9071 62 39 20 Fair20 0.7362 0.9554 74 64 32 Good21 0.4990 0.9125 63 40 22 Faira – a comparison of extant and total community similarityb – not calculated beause too few mussels were collected27

Table 5. Water quality measurements from the 21 sample locations on Big Walnut Creek.Site # Water Conductivity O 2 Turbidity pH DateTemp o C uS/cm mg/l NTU1 ----- ---- ----- 4.66 ----- 1 Sept. 20122 20.1 374 6.20 11.30 8.20 9 Sept. 20123 19.2 339 7.12 17.70 8.33 22 Sept. 20124 16.1 394 7.39 8.74 8.34 29 Sept. 20125 16.1 394 7.39 8.74 8.34 29 Sept. 20126 16.1 394 7.39 8.74 8.34 29 Sept. 20127 17.3 487 8.21 5.50 8.57 30 Sept. 20128 17.3 487 8.21 5.50 8.57 30 Sept. 20129 17.7 389 6.99 4.64 8.26 4 Oct. 201210 17.7 389 6.99 4.64 8.26 4 Oct. 201211 10.4 539 8.96 2.85 8.30 11 Oct. 201212 10.4 539 8.96 2.85 8.30 11 Oct. 201213 21.4 578 6.96 8.28 8.36 13 Sept. 201214 21.4 578 6.96 8.28 8.36 13 Sept. 201215 19.2 656 7.46 6.65 8.41 15 Sept. 201216 19.2 656 7.46 6.65 8.41 16 Sept. 201217 23.3 628 8.32 5.22 8.60 16 Sept. 201218 ----- ---- ----- ----- ----- 13 Oct. 201219 19.3 677 8.17 5.46 8.67 20 Sept. 201220 19.3 677 8.17 5.46 8.67 20 Sept. 201221 19.3 677 8.17 5.46 8.67 20 Sept. 201228

Table 6. Mussels recently collected from the Scioto River near the mouth of Big Walnut Creekby Hoggarth (2004, 2008a, 2009a, 2009b).Species PIC 22 PIC 4 FRA 270 Southerly# % # % # % # %1. Utterbackia imbecillis 1 0.6 0 0.0 1 0.2 0 0.02. Anodonta subrotunda 1 0.6 0 0.0 1 0.2 0 0.03. Pyganodon grandis 0 0.0 0 0.0 4 0.6 1 0.14. Alasmidonta marginata 0 0.0 0 0.0 0 0.0 2 0.35. Lasmigona costata 1 0.6 0 0.0 0 0.0 0 0.06. Lasmigona complanata 12 6.9 10 6.2 75 11.8 45 6.27. Amblema plicatata 4 2.3 0 0.0 7 1.1 4 0.68. Tritogonia verrucosa 22 12.6 1 0.6 1 0.2 2 0.39. Quadrula quadrula 27 15.5 63 39.1 178 28.1 242 33.310. Quadrula pustulosa 1 0.6 4 2.5 1 0.2 4 0.611. Fusconaia flava 1 0.6 0 0.0 0 0.0 0 0.012. Leptodea fragilis 52 29.9 0 0.0 152 24.0 135 18.613. Potamilus alatus 2 1.2 9 5.6 9 1.4 28 3.914. Potamilus ohiensis 2 1.2 7 4.3 0 0.0 2 0.315. Truncilla donaciformis 15 8.6 27 16.8 157 24.8 233 32.116. Truncilla truncata 8 4.6 15 9.3 43 6.8 16 2.217. Obliquaria reflexa 6 3.5 24 14.9 2 0.3 8 1.118. Toxolasma parvum 0 0.0 0 0.0 1 0.2 0 0.019. Ligumia recta 3 1.7 0 0.0 1 0.2 1 0.120. Lampsilis r. luteola 2 1.2 0 0.0 0 0.0 1 0.121. Lampsilis cardium 12 6.9 1 0.6 1 0.2 3 0.422. Lampsilis fasciola 2 1.2 0 0.0 0 0.0 0 0.0Total 174 161 634 727PIC 22 = US Rt. 22, Pickaway County, 15 miles downstream of the mouth of Big Walnut Creek(Hoggarth, 2004)PIC 4 = CO Rt. 4, Pickaway County, 13 miles downstream of the mouth of Big Walnut Creek(Hoggarth, 2009a)FRA 270 = IR Rt. 270, Franklin County, 7 miles upstream of the mouth of Big Walnut Creek(Hoggarth, 2008)Southerly = Southerly Wastewater Treatment Plant relocation area at the mouth of Big WalnutCreek (Hoggarth, 2009b)29

Figure 1. Map of the Big Walnut Creek watershed from Hoover Dam to its mouth showing the21 sample locations, the canoed reaches (red) and where access was from bridges (blue).30

ABCDEFFigure 2. Extant state significant species of mussels in Big Walnut Creek collected in 2012.A – Megalonaias nervosa (Ohio endangered), B – Ligumia recta (Ohio Threatened), and C –Alasmidonta marginata, D – Ptychobranchus fasciolaris, E – Truncilla truncata, and F –Lampsilis fasciola (top, female, bottom male) (all Ohio Special Interest).31

Number of Individuals140120100Extant80Extirpated60402001 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21Site NumberFigure 3. Line graph showing the relationship between the number of extant and extirpated individuals collected from each collectionsite from Big Walnut Creek from below Hoover Reservoir (Site 1) to immediately above its mouth (Site 21).32

Number of Species18161412ExtantExirpated10864201 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21Site NumberFigure 4. Line graph showing the relationship between the number of extant and extirpated species collected from each collection sitefrom Big Walnut Creek from below Hoover Reservoir (Site 1) to immediately above its mouth (Site 21).33

Average difference between H'-Extant & H'-Total0.45b0.40.35b0.30.250.2a0.150.10.0501 2 31 - Upstream sites 2 - Midstream sites 3 - Downstream sitesFigure 5. Average difference between the total community (H’-Total) and the extant community (H’-Extant) for the musselcommunites of Big Walnut Creek. The upstream sites are sites 1-7, the midstream sites are sites 8-14, and the downstream sites aresites 15-21. a = no significant difference between the H’-Total and H’-Extant and b shows a significant difference between the twomeasures of community diversity (p < 0.05)34

H' Total1.41.210.80.60.4y = 0.5418x + 0.4994R² = 0.3343, p > 0.050.200 0.2 0.4 0.6 0.8 1 1.2H' ExtantFigure 6. Relationship between H’ (Shannon-Weiner Diversity Index) for the total community (live, freshly dead, weathered andsubfossil) compared to extant (live and freshly dead).35

Mussel- IBI4540353025201510y = 43.932x - 5.5806R² = 0.6008, p < 0.05500 0.2 0.4 0.6 0.8 1 1.2Jaccard Coeficient of SimilarityFigure 7. Relationship between Mussel-IBI scores and how similar the total community was to the extant community (JaccardCoeficient of Similarity).36

Mussel-IBI403530252015y = 25.986x + 11.01R² = 0.6864, p < 0.0510500.00 0.20 0.40 0.60 0.80 1.00 1.20% Extant SpeciesFigure 8. Relationship between Mussel-IBI and the % extant species of mussels.37

Mussel-IBI40353025201510y = 0.184x + 21.05R² = 0.0367, p > 0.05500 10 20 30 40 50 60Fish-IBI (OEPA, 2003)Figure 9. Relationship between Mussel-IBI (this study) and Fish-IBI (OEPA, 2004).38

Figure 10. OEPA (2003) Figure 50 showing IBI and MIwb scoresfor Big Walnut Creek from 1982 to 2000.39

Site 1: Big Walnut Creek (R.M. 36.7) downstream of Central College Road, at 40 o 05’54.5”N,82 o 52’50.5”W, 40.097607 o N -82.880414 o W, Franklin Co., Ohio, on 1 September 2012 and 2September 2012.Live mussels: Species Common name Length General Transect Age1. Pyganodon grandis common floater --- x ---2. Elliptio dilatata spike 92 x 153. Elliptio dilatata spike 93 x 124. Elliptio dilatata spike 85 x 125. Fusconaia flava Wabash pigtoe 75 x 146. Ptychobranchus fasciolaris kidneyshell 88 x 157. Lampsilis cardium plain pocketbook 90 x 128. Fusconaia flava Wabash pigtoe 69 T2Q6 109. Lampsilis r. luteola fatmucket 80 T2Q19 9Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona costata fluted-shell 3 0 5 82. Amblema plicata threeridge 0 0 1 13. Fusconaia flava Wabash pigtoe 4 6 0 104. Elliptio dilatata spike 11 6 0 175. Ptychobranchus fasciolaris kidneyshell 4 0 0 4Total 22 12 6 40Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 40/0 Q2: 60/0 Q3: 35/0 Q4: 40/0Q5: 25/0 Q6: 50/0 Q7: 60/0 Q8: 50/0Q9: 40/0 Q10: 40/0 Q11: 35/0 Q12: 40/0Q13: 35/0 Q14: 20/0 Q15: 20/0 Q16: 15/0Q17: 20/0 Q18: 15/0 Q19: 15/0 Q20: 15/0Total # of C. fluminea = 670: Density = 134.0/m 2Total # of mussels = 0: Density = 0.0/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 35/0 Q2: 50/0 Q3: 50/0 Q4: 50/0Q5: 50/0 Q6: 35/1 Q7: 50/0 Q8: 35/0Q9: 20/0 Q10: 50/0 Q11: 25/0 Q12: 50/0Q13: 20/0 Q14: 20/0 Q15: 50/0 Q16: 20/0Q17: 50/0 Q18: 20/0 Q19: 25/1 Q20: 0/0Total # of C. fluminea = 705: Density = 141.0/m 2Total # of mussels = 2: Density = 0.4/m 241

Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 1 0 0 02. Lasmigona costata fluted-shell 0 3 0 53. Amblema plicata threeridge 0 0 0 14. Fusconaia flava Wabash pigtoe 2 4 6 05. Elliptio dilatata spike 3 11 6 06. Ptychobranchus fasciolaris kidneyshell 1 4 0 07. Lampsilis r. luteola fat mucket 1 0 0 08. Lampsilis cardium plain pocketbook 1 0 0 0Total number of individual 9 22 12 6Total individual 1) live + dead, 2) total 31 49H’ 0.6642 0.6761Jaccard 0.89% Extant species 0.88Mussel-IBI 2842

Site 2: Big Walnut Creek (R.M. 32.1) downstream of Morse Road, at 40 o 03’40.1”N,82 o 53’27.3”W, 40.051029 o N -82.890739 o W, Franklin Co., Ohio, on 9 September 2012.Live mussels: Species Common name Length General Transect Age1. Pyganodon grandis giant floater --- x ---2. Leptodea fragilis fragile papershell --- x ---3. Lampsilis r. luteola fatmucket --- x ---4. Lampsilis cardium plain pocketbook --- x ---5. Elliptio dilatata spike 85 T1Q3 106. Elliptio dilatata spike 81 T1Q4 107. Elliptio dilatata spike 92 T1Q6 138. Elliptio dilatata spike 93 T1Q7 139. Elliptio dilatata spike 81 T1Q12 910. Elliptio dilatata spike 96 T1Q12 1511. Leptodea fragilis fragile papershell 73 T1Q12 512. Lampsilis r. luteola fatmucket 87 T1Q12 613. Lampsilis cardium plain pocketbook 98 T1Q14 1014. Fusconaia flava Wabash pigtoe 98 T1Q17 1515. Elliptio dilatata spike 95 T1Q19 1516. Elliptio dilatata spike 84 T2Q11 1517. Elliptio dilatata spike 84 T2Q11 1518. Elliptio dilatata spike 90 T2Q11 1519. Elliptio dilatata spike 86 T2Q13 1220. Elliptio dilatata spike 82 T2Q13 1221. Leptodea fragilis fragile papershell 86 T2Q14 1722. Elliptio dilatata spike 86 T2Q14 1223. Elliptio dilatata spike 86 T2Q18 1324. Elliptio dilatata spike 85 T2Q20 12Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona costata fluted-shell 0 2 0 22. Lasmigona complanata white heelsplitter 0 0 1 13. Amblema plicata threeridge 0 0 1 14. Quadrula quadrula mapleleaf 0 1 0 15. Fusconaia flava Wabash pigtoe 0 2 0 26. Elliptio dilatata spike 0 9 0 97. Ptychobranchus fasciolaris kidneyshell 0 2 0 28. Leptodea fragilis fragile papershell 0 1 0 19. Lampsilis r. luteola fatmucket 0 1 0 110. Lampsilis cardium plain pocketbook 0 2 0 2Total 0 20 2 2243

Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 10/0 Q2: 7/0 Q3: 5/1 Q4: 11/1Q5: 13/0 Q6: 13/1 Q7: 7/1 Q8: 2/0Q9: 2/0 Q10: 7/0 Q11: 2/0 Q12: 6/3Q13: 4/0 Q14: 3/1 Q15: 16/0 Q16: 3/0Q17: 1/1 Q18: 1/0 Q19: 3/1 Q20: 0/0Total # of C. fluminea = 116: Density = 23.2/m 2Total # of mussels = 11: Density = 2.2/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 0/0 Q2: 3/0 Q3: 2/0 Q4: 6/0Q5: 6/0 Q6: 0/0 Q7: 4/0 Q8: 4/0Q9: 1/0 Q10: 2/0 Q11: 1/3 Q12: 0/0Q13: 2/2 Q14: 7/2 Q15: 6/0 Q16: 12/0Q17: 6/0 Q18: 0/1 Q19: 2/0 Q20: 5/1Total # of C. fluminea = 69: Density = 13.8/m 2Total # of mussels = 9: Density = 1.8/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 1 0 0 02. Lasmigona costata fluted-shell 0 0 2 03. Lasmigona complanata white heelsplitter 0 0 0 14. Amblema plicata threeridge 0 0 0 15. Quadrula quadrula mapleleaf 0 0 1 06. Fusconaia flava Wabash pigtoe 1 0 2 07. Elliptio dilatata spike 15 0 9 08. Ptychobranchus fasciolaris kidneyshell 0 0 2 09. Leptodea fragilis paper pondshell 3 0 1 010. Lampsilis r. luteola fat mucket 2 0 1 011. Lampsilis cardium plain pocketbook 2 0 2 0Total number of individual 24 0 20 2Total individual 1) live + dead, 2) total 24 46H’ 0.5353 0.7495Jaccard coefficient of similarity (% similar – total & extant communities) 69% Extant species 55Mussel-IBI 2444

Site 3: Big Walnut Creek (R.M. 30.9) at Woodside Green Park, at 40 o 02’38.7”N, 82 o 52’47.9”W,40.044091 o N -82.879978 o W, Franklin Co., Ohio, on 22 September 2012.Live mussels: Species Common name Length General Transect Age1. Fusconaia flava Wabash pigtoe 44 x 52. Fusconaia flava Wabash pigtoe 52 x 93. Fusconaia flava Wabash pigtoe 58 x 124. Elliptio dilatata spike 42 x 45. Elliptio dilatata spike 51 x 56. Elliptio dilatata spike 49 x 67. Elliptio dilatata spike 49 x 68. Elliptio dilatata spike 52 x 69. Elliptio dilatata spike 58 x 810. Ptychobranchus fasciolaris kidneyshell 55 x 511. Ptychobranchus fasciolaris kidneyshell 64 x 612. Ptychobranchus fasciolaris kidneyshell 88 x 1413. Ptychobranchus fasciolaris kidneyshell 103 x 1714. Villosa iris ♂ rainbow 51 x 715. Villosa iris ♂ rainbow 37 x 416. Villosa iris ♀ rainbow 41 x 717. Lampsilis r. luteola ♀ fat mucket 62 x 518. Lampsilis r. luteola ♀ fat mucket 65 x 519. Elliptio dilatata spike 55 T1Q1 620. Ptychobranchus fasciolaris kidneyshell 62 T1Q1 621. Leptodea fragilis juv. fragile papershell 33 T1Q1 222. Lampsilis r. luteola ♂ fat mucket 58 T1Q1 323. Lampsilis fasciola juv. wavy-rayed poketbook 30 T1Q2 224. Elliptio dilatata spike 50 T1Q3 625. Elliptio dilatata spike 40 T1Q4 526. Elliptio dilatata spike 46 T1Q4 527. Elliptio dilatata spike 78 T1Q4 1528. Villosa iris ♂ rainbow 42 T1Q4 429. Villosa iris ♀ rainbow 48 T1Q4 730. Lampsilis r. luteola ♀ fat mucket 65 T1Q4 531. Lampsilis r. luteola ♂ fat mucket 80 T1Q4 832. Ptychobranchus fasciolaris kidneyshell 75 T1Q5 833. Lampsilis r. luteola ♀ fat mucket 55 T1Q5 434. Fusconaia flava Wabash pigtoe 54 T1Q6 935. Fusconaia flava Wabash pigtoe 66 T1Q6 1436. Fusconaia flava Wabash pigtoe 81 T1Q6 1937. Elliptio dilatata spike 43 T1Q6 238. Truncilla truncata deertoe 10 T1Q6 139. Elliptio dilatata spike 38 T1Q7 540. Ptychobranchus fasciolaris kidneyshell 58 T1Q7 641. Ptychobranchus fasciolaris kidneyshell 69 T1Q7 945

42. Lampsilis r. luteola ♀ fat mucket 66 T1Q7 643. Elliptio dilatata spike 45 T1Q8 444. Elliptio dilatata spike 59 T1Q8 645. Elliptio dilatata spike 80 T1Q8 1346. Ptychobranchus fasciolaris kidneyshell 15 T1Q8 147. Fusconaia flava Wabash pigtoe 80 T1Q9 1848. Elliptio dilatata spike 35 T1Q9 449. Elliptio dilatata spike 55 T1Q9 650. Elliptio dilatata spike 66 T1Q9 751. Lampsilis r. luteola ♂ fat mucket 62 T1Q9 552. Fusconaia flava Wabash pigtoe 49 T1Q10 753. Elliptio dilatata spike 66 T1Q10 654. Ptychobranchus fasciolaris kidneyshell 69 T1Q10 855. Lampsilis fasciola ♂ wavy-rayed pocketbook 35 T1Q10 456. Lampsilis fasciola ♀ wavy-rayed pocketbook 42 T1Q10 557. Lampsilis r. luteola ♂ fat mucket 70 T1Q10 758. Ptychobranchus fasciolaris kidneyshell 73 T1Q11 1259. Fusconaia flava Wabash pigtoe 82 T1Q12 1660. Elliptio dilatata spike 46 T1Q12 461. Elliptio dilatata spike 56 T1Q12 662. Elliptio dilatata spike 58 T1Q12 763. Elliptio dilatata spike 60 T1Q12 764. Ptychobranchus fasciolaris kidneyshell 93 T1Q12 1365. Villosa iris ♂ rainbow 47 T1Q12 466. Lampsilis r. luteola ♂ fat mucket 99 T1Q12 1067. Elliptio dilatata spike 49 T1Q13 568. Elliptio dilatata spike 81 T1Q13 1269. Villosa iris ♀ rainbow 48 T1Q13 470. Elliptio dilatata spike 88 T1Q15 771. Lampsilis r. luteola ♂ fat mucket 50 T1Q15 372. Elliptio dilatata spike 52 T1Q17 673. Lampsilis r. luteola ♂ fat mucket 74 T1Q17 674. Elliptio dilatata spike 88 T1Q18 1675. Villosa iris ♂ rainbow 55 T1Q18 676. Elliptio dilatata spike 25 T1Q19 277. Elliptio dilatata spike 55 T1Q19 678. Elliptio dilatata spike 57 T1Q19 679. Ptychobranchus fasciolaris kidneyshell 64 T1Q19 780. Villosa iris ♂ rainbow 44 T1Q19 681. Lampsilis r. luteola ♂ fat mucket 77 T1Q19 1682. Elliptio dilatata spike 40 T1Q20 483. Elliptio dilatata spike 51 T1Q20 584. Elliptio dilatata spike 52 T1Q20 685. Elliptio dilatata spike 55 T1Q20 686. Ptychobranchus fasciolaris kidneyshell 20 T1Q20 187. Ptychobranchus fasciolaris kidneyshell 65 T1Q20 746

88. Lampsilis fasciola ♀ wavy-rayed pocketbook 40 T1Q20 289. Lampsilis r. luteola ♀ fat mucket 58 T2Q1 290. Elliptio dilatata spike 69 T2Q3 1291. Toxolasma parvum lilliput 15 T2Q3 492. Ptychobranchus fasciolaris kidneyshell 70 T2Q4 893. Elliptio dilatata spike 42 T2Q5 694. Obovaria subrotunda hickorynut 40 T2Q5 795. Elliptio dilatata spike 71 T2Q7 1296. Elliptio dilatata spike 80 T2Q7 1397. Lampsilis r. luteola ♂ fat mucket 64 T2Q8 698. Elliptio dilatata spike 74 T2Q9 1299. Elliptio dilatata spike 87 T2Q9 14100. Obovaria subrotunda hickorynut 42 T2Q10 8101. Lampsilis r. luteola ♂ fat mucket 28 T2Q10 3102. Lampsilis r. luteola ♂ fat mucket 53 T2Q10 3103. Lampsilis r. luteola ♂ fat mucket 60 T2Q10 4104. Lampsilis r. luteola ♂ fat mucket 67 T2Q10 9105. Elliptio dilatata spike 44 T2Q12 6106. Elliptio dilatata spike 60 T2Q12 9107. Elliptio dilatata spike 67 T2Q12 11108. Fusconaia flava Wabash pigtoe 80 T2Q13 12109. Ptychobranchus fasciolaris kidneyshell 61 T2Q13 6110. Fusconaia flava Wabash pigtoe 65 T2Q14 11111. Elliptio dilatata spike 65 T2Q14 6112. Elliptio dilatata spike 44 T2Q15 6113. Elliptio dilatata spike 62 T2Q15 8114. Lampsilis r. luteola ♂ fat mucket 49 T2Q15 3115. Elliptio dilatata spike 36 T2Q19 4116. Elliptio dilatata spike 69 T2Q19 12117. Elliptio dilatata spike 70 T2Q19 11118. Lampsilis r. luteola ♂ fat mucket 42 T2Q20 6Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona costata fluted-shell 0 1 0 12. Fusconaia flava Wabash pigtoe 3 0 0 33. Elliptio dilatata spike 3 0 0 34. Ptychobranchus fasciolaris kidneyshell 2 1 0 35. Villosa iris rainbow 2 0 0 26. Lampsilis r. luteola fat mucket 3 1 0 47. Lampsilis cardium plain pocketbook 0 1 0 18. Lampsilis fasciola wavy-rayed pocket. 1 0 0 1Total 14 4 0 1847

Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 27/4 Q2: 21/1 Q3: 25/1 Q4: 12/7Q5: 20/2 Q6: 29/5 Q7: 22/4 Q8: 28/4Q9: 21/5 Q10: 10/6 Q11: 8/1 Q12: 13/8Q13: 18/3 Q14: 15/0 Q15: 12/2 Q16: 18/0Q17: 15/2 Q18: 11/2 Q19: 19/6 Q20: 23/7Total # of C. fluminea = 367: Density = 73.4/m 2Total # of mussels = 70: Density = 14.0/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 40/1 Q2: 20/0 Q3: 25/2 Q4: 30/1Q5: 35/2 Q6: 37/0 Q7: 23/2 Q8: 18/1Q9: 15/2 Q10: 22/5 Q11: 36/0 Q12: -- /3Q13: --/2 Q14: --/2 Q15: --/3 Q16: 15/0Q17: 40/0 Q18: 42/0 Q19: --/3 Q20: --/1Total # of C. fluminea = 398/3.5 m 2 : Density = 73.4/m 2Total # of mussels = 30: Density = 6.0/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Lasmigona costata fluted-shell 0 0 1 02. Fusconaia flava Wabash pigtoe 11 3 0 03. Elliptio dilatata spike 51 3 0 04. Ptychobranchus fasciolaris kidneyshell 17 2 1 05. Leptodea fragilis paper pondshell 1 0 0 06. Toxolasma parvum Lilliput 1 0 0 07. Truncilla truncata deertoe 1 0 0 08. Obovaria subrotunda hickorynut 2 0 0 09. Villosa iris rainbow 9 2 0 010. Lampsilis r. luteola fat mucket 21 3 1 011. Lampsilis cardium plain pocketbook 0 0 1 012. Lampsilis fasciola wavy-rayed pocket. 4 1 0 0Total number of individual 118 14 4 0Total individual 1) live + dead, 2) total 132 136H’ 0.7310 0.7593Jaccard coefficient of similarity (% similar – total & extant communities) 86% Extant species 83Mussel-IBI 3448

Site 4: Big Walnut Creek (R.M. 29.1) adjacent to Gahanna Municipal Golf Course, at40 o 01’47.3”N, -82 o 52’40.2”W, 40.029562 o N 82.877703 o W, Franklin Co., Ohio, on 29September 2012.Live mussels: Species Common name Length General Transect Age1. Lasmigona costata fluted-shell 115 x 142. Amblema plicata threeridge 99 x 103. Amblema plicata threeridge 113 x 154. Quadrula quadrula mapleleaf 63 x 85. Quadrula quadrula mapleleaf 71 x 96. Quadrula quadrula mapleleaf 89 x 107. Fusconaia flava Wabash pigtoe 55 x 68. Fusconaia flava Wabash pigtoe 81 x 109. Elliptio dilatata spike 36 x 210. Elliptio dilatata spike 43 x 311. Lampsilis r. luteola ♀ fat mucket 67 x 412. Lampsilis r. luteola ♂ fat mucket 111 x 1913. Lampsilis cardium ♀ plain pocketbook ---- x ---14. Lampsilis fasciola ♀ wavy-rayed pocketbook 47 x 515. Fusconaia flava Wabash pigtoe 80 T1Q11 916. Elliptio dilatata spike 113 T1Q11 2017. Fusconaia flava Wabash pigtoe 74 T1Q12 1418. Ptychobranchus fasciolaris kidneyshell 80 T1Q12 1019. Amblema plicata threeridge 113 T1Q13 2020. Elliptio dilatata spike 75 T1Q13 1121. Ptychobranchus fasciolaris kidneyshell 43 T1Q14 422. Ptychobranchus fasciolaris kidneyshell 100 T1Q14 1223. Ptychobranchus fasciolaris kidneyshell 99 T1Q15 1424. Amblema plicata threeridge 79 T1Q16 1025. Elliptio dilatata spike 92 T1Q17 1326. Ptychobranchus fasciolaris kidneyshell 63 T1Q17 827. Lampsilis cardium ♂ plain pocketbook 93 T1Q17 1428. Elliptio dilatata spike 42 T1Q18 429. Lampsilis cardium ♀ plain pocketbook 84 T1Q19 830. Ptychobranchus fasciolaris kidneyshell 63 T1Q20 531. Ptychobranchus fasciolaris kidneyshell 91 T1Q15 1332. Lampsilis r. luteola ♀ fat mucket 64 T1Q20 533. Ptychobranchus fasciolaris kidneyshell 44 T2Q4 334. Lampsilis r. luteola ♀ fat mucket 64 T2Q5 335. Villosa iris juv. rainbow 21 T2Q6 136. Ptychobranchus fasciolaris kidneyshell 41 T2Q10 437. Leptodea fragilis ♀ fragile papershell 63 T2Q11 238. Fusconaia flava Wabash pigtoe 51 T2Q13 839. Fusconaia flava Wabash pigtoe 74 T2Q13 1040. Ptychobranchus fasciolaris kidneyshell 32 T2Q13 149

41. Elliptio dilatata spike 83 T2Q14 1042. Lampsilis cardium ♀ plain pocketbook 125 T2Q16 2543. Elliptio dilatata spike 84 T2Q18 1044. Elliptio dilatata spike 35 T2Q19 245. Elliptio dilatata spike 66 T2Q19 746. Elliptio dilatata spike 94 T2Q19 1347. Lampsilis r. luteola ♂ fat mucket 69 T2Q19 548. Elliptio dilatata spike 84 T2Q20 1049. Lampsilis cardium ♀ plain pocketbook 86 T2Q20 4Dead mussels: Species Common name Dead Weathered Subfossil Total1. Pygaonodon grandis giant floater 0 1 0 12. Strophitus undulatus creeper 0 2 0 23. Lasmigona costata fluted-shell 0 1 0 14. Amblema plicata threeridge 0 2 0 25. Fusconaia flava Wabash pigtoe 1 4 0 56. Elliptio dilatata spike 1 5 0 67. Ptychobranchus fasciolaris kidneyshell 1 2 0 38. Obovaria subrotunda hickorynut 0 3 0 39. Lampsilis r. luteola fat mucket 1 0 0 110. Epioblasm triquetra snuffbox 0 1 0 1Total 4 21 0 25Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 6/0 Q2: 7/0 Q3: 11/0 Q4: 17/0Q5: 4/0 Q6: 23/0 Q7: 24/0 Q8: 23/0Q9: 36/0 Q10: 25/0 Q11: 35/2 Q12: 22/2Q13: 20/2 Q14: 22/2 Q15: 18/1 Q16: 32/1Q17: 28/3 Q18: 23/1 Q19: 28/1 Q20: 26/3Total # of C. fluminea = 429: Density = 85.8/m 2Total # of mussels = 18: Density = 3.6/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 24/0 Q2: 14/0 Q3: 25/0 Q4: 29/1Q5: 5/1 Q6: 35/1 Q7: 39/0 Q8: 35/0Q9: 31/0 Q10: 25/1 Q11: 13/1 Q12: 15/0Q13: 15/3 Q14: 13/1 Q15: 11/0 Q16: 10/1Q17: 19/0 Q18: 15/1 Q19: 7/4 Q20: 7/2Total # of C. fluminea = 387: Density = 77.4/m 2Total # of mussels = 17: Density = 3.4/m 250

Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 0 0 1 02. Strophitus undulatus creeper 0 0 2 03. Lasmigona costata fluted-shell 1 0 1 04. Amblema plicata threeridge 4 0 2 05. Quadrula quadrula mapleleaf 3 0 0 06. Fusconaia flava Wabash pigtoe 6 1 4 07. Elliptio dilatata spike 12 1 5 08. Ptychobranchus fasciolaris kidneyshell 10 1 2 09. Leptodea fragilis paper pondshell 1 0 0 010. Obovaria subrotunda hickorynut 0 0 3 011. Villosa iris rainbow 1 0 0 012. Lampsilis r. luteola fat mucket 5 1 0 013. Lampsilis cardium plain pocketbook 5 0 0 014. Lampsilis fasciola wavy-rayed pocket. 1 0 0 015. Epioblasm triquetra snuffbox 0 0 1 0Total number of individual 49 4 21 0Total individual 1) live + dead, 2) total 53 74H’ 0.8975 0.9849Jaccard coefficient of similarity (% similar – total & extant communities) 79% Extant species 73Mussel-IBI 3651

Site 5: Big Walnut Creek (R.M. 27.7) at the IR 270 Bridge, at 40 o 01’47.3”N, 82 o 52’40.2”W,40.006664 o N -82.874567 o W, Franklin Co., Ohio, on 29 September 2012.Live mussels: Species Common name Length General Transect Age1. Pyganodon grandis giant floater 100 x 62. Lasmigona costata fluted-shell 94 x 83. Fusconaia flava Wabash pigtoe 89 x 184. Ptychobranchus fasciolaris kidneyshell 57 x 25. Lampsilis r. luteola ♀ fat mucket 59 x 36. Lampsilis r. luteola ♀ fat mucket 77 x 77. Lampsilis r. luteola ♀ fat mucket 81 x 148. Lampsilis r. luteola ♂ fat mucket 88 x 109. Lampsilis r. luteola ♂ fat mucket 90 x 1410. Lampsilis cardium ♂ plain pocketbook 110 x 1711. Lampsilis cardium ♀ plain pocketbook ---- x ---12. Lampsilis cardium ♀ plain pocketbook ---- x ---13. Lampsilis cardium ♀ plain pocketbook ---- x ---14. Lampsilis cardium ♂ plain pocketbook ---- x ---15. Lampsilis fasciola ♀ wavy-rayed pocketbook 61 x 1016. Lampsilis fasciola ♀ wavy-rayed pocketbook --- x ---17. Lampsilis fasciola ♀ wavy-rayed pocketbook --- x ---18. Lampsilis fasciola ♀ wavy-rayed pocketbook --- x ---19. Fusconaia flava Wabash pigtoe 42 T1Q6 420. Elliptio dilatata creeper 70 T1Q10 821. Fusconaia flava Wabash pigtoe 60 T1Q18 12Dead mussels: Species Common name Dead Weathered Subfossil Total1. Strophitus undulatus creeper 1 0 0 12. Lasmigona costata fluted-shell 3 0 0 33. Lasmigona complanata white heelsplitter 1 0 0 14. Quadrula quadrula mapleleaf 0 1 0 15. Fusconaia flava Wabash pigtoe 3 0 0 36. Ptychobranchus fasciolaris kidneyshell 5 0 0 57. Villosa iris rainbow 1 0 0 18. Lampsilis r. luteola fat mucket 5 0 0 59. Lampsilis cardium plain pocketbook 2 0 0 210. Lampsilis fasciola wavy-rayed pocketbook 1 0 0 111. Epioblasm triquetra snuffbox 0 0 1 1Total 22 1 1 2452

Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 10/0 Q2: 17/0 Q3: 16/0 Q4: 12/0Q5: 27/0 Q6: 16/1 Q7: 10/0 Q8: 8/0Q9: 14/0 Q10: 17/1 Q11: 8/0 Q12: 14/0Q13: 28/0 Q14: 29/0 Q15: 18/0 Q16: 14/0Q17: 7/0 Q18: 14/1 Q19: 12/0 Q20: 12/0Total # of C. fluminea = 303: Density = 60.6/m 2Total # of mussels = 3: Density = 0.6/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 8/0 Q2: 10/0 Q3: 5/0 Q4: 4/0Q5: 7/0 Q6: 8/0 Q7: 0/0 Q8: 7/0Q9: 12/0 Q10: 0/0 Q11: 0/0 Q12: 3/0Q13: 6/0 Q14: 8/0 Q15: 9/0 Q16: 8/0Q17: 8/0 Q18: 6/0 Q19: 4/0 Q20: 7/0Total # of C. fluminea = 120: Density = 24.0/m 2Total # of mussels = 0: Density = 0.0/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 1 0 0 02. Strophitus undulatus creeper 0 1 0 03. Lasmigona costata fluted-shell 1 3 0 04. Lasmigona complanata white heelsplitter 0 1 0 05. Quadrula quadrula mapleleaf 0 0 1 06. Fusconaia flava Wabash pigtoe 3 3 0 07. Elliptio dilatata spike 1 0 0 08. Ptychobranchus fasciolaris kidneyshell 1 5 0 09. Villosa iris rainbow 0 1 0 010. Lampsilis r. luteola fat mucket 5 5 0 011. Lampsilis cardium plain pocketbook 5 2 0 012. Lampsilis fasciola wavy-rayed pocket. 4 1 0 013. Epioblasm triquetra snuffbox 0 0 0 1Total number of individual 21 22 1 1Total individual 1) live + dead, 2) total 43 45H’ 0.9089 0.9608Jaccard coefficient of similarity (% similar – total & extant communities) 87% Extant species 85Mussel-IBI 3253

Site 6: Big Walnut Creek (R.M. 26.2) at Airport Golf Course downstream of Pizzuro Park, at40 o 01’47.3”N, 82 o 52’40.2”W, 39.993509 o N -82.857756 o W, Franklin Co., Ohio, on 29September 2012.Live mussels: Species Common name Length General Transect Age1. Lasmigona costata fluted-shell 91 x 82. Amblema plicata threeridge 105 x 103. Fusconaia flava Wabash pigtoe 74 x 144. Fusconaia flava Wabash pigtoe 81 x 125. Ptychobranchus fasciolaris kidneyshell 99 x 156. Leptodea fragilis fragile papershell 75 x 47. Leptodea fragilis fragile papershell 87 x 108. Leptodea fragilis fragile papershell 104 x 79. Lampsilis r. luteola juv fat mucket 37 x 210. Lampsilis r. luteola ♂ fat mucket 51 x 311. Lampsilis r. luteola ♂ fat mucket 61 x 412. Lampsilis r. luteola ♂ fat mucket 62 x 413. Lampsilis r. luteola ♂ fat mucket 91 x 1214. Lampsilis r. luteola ♂ fat mucket 94 x 1415. Lampsilis r. luteola ♂ fat mucket 97 x 1416. Lampsilis cardium ♀ plain pocketbook 75 x 717. Lampsilis cardium ♀ plain pocketbook 95 x 918. Lampsilis cardium ♀ plain pocketbook 95 x 919. Lampsilis cardium ♀ plain pocketbook 104 x 1420. Lampsilis cardium ♀ plain pocketbook 104 x 1421. Lampsilis cardium ♀ plain pocketbook 110 x 1422. Lampsilis cardium ♂ plain pocketbook 117 x 2023. Lampsilis cardium ♀ plain pocketbook 120 x 1524. Lampsilis cardium ♂ plain pocketbook 125 x 2225. Lampsilis cardium ♂ plain pocketbook 125 x 22Dead mussels: Species Common name Dead Weathered Subfossil Total1. Amblema plicata threeridge 0 1 2 32. Quadrula quadrula mapleleaf 0 0 1 13. Fusconaia flava Wabash pigtoe 0 1 0 14. Ptychobranchus fasciolaris kidneyshell 0 1 0 15. Leptodea fragilis fragile papershell 3 0 0 36. Obovaria subrotunda hickorynut 0 1 0 17. Lampsilis cardium plain pocketbook 0 1 0 18. Epioblasm triquetra snuffbox 0 1 0 1Total 3 6 3 1254

Transect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Lasmigona costata fluted-shell 1 0 0 02. Amblema plicata threeridge 1 0 1 23. Quadrula quadrula mapleleaf 0 0 0 14. Fusconaia flava Wabash pigtoe 2 0 1 05. Ptychobranchus fasciolaris kidneyshell 1 0 1 06. Leptodea fragilis paper pondshell 3 3 0 07. Obovaria subrotunda hickorynut 0 0 1 08. Lampsilis r. luteola fat mucket 7 0 0 09. Lampsilis cardium plain pocketbook 10 0 1 010. Epioblasm triquetra snuffbox 0 0 1 0Total number of individual 25 3 6 3Total individual 1) live + dead, 2) total 28 37H’ 0.7068 0.8694Jaccard coefficient of similarity (% similar – total & extant communities) 77% Extant species 70Mussel-IBI* 28* quadrat sampling not done so % invasive bivavles given value of 155

Site 7: Big Walnut Creek (R.M. 24.0) at Whitehall Community Center, at 39 o 58’54.5”N,82 o 51’50.9”W, 39.981531 o N -82.863957 o W, Franklin Co., Ohio, on 30 September 2012.Live mussels: Species Common name Length General Transect Age1. Pyganodon grandis giant floater 120 x 142. Elliptio dilatata spike 82 x 223. Leptodea fragilis fragile papershell 90 x 94. Leptodea fragilis fragile papershell 105 x 115. Leptodea fragilis fragile papershell 121 x 186. Lampsilis r. luteola ♀ fat mucket 67 x 77. Lampsilis r. luteola ♀ fat mucket 69 x 68. Lampsilis r. luteola ♂ fat mucket 83 x 8Dead mussels: Species Common name Dead Weathered Subfossil Total1. Amblema plicata threeridge 0 0 1 12. Fusconaia flava Wabash pigtoe 0 1 3 43. Elliptio dilatata spike 0 2 2 44. Leptodea fragilis fragile papershell 2 0 0 25. Ligumia recta black sandshell 0 0 1 16. Lampsilis cardium plain pocketbook 1 0 0 1Total 3 3 7 13Transect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 256

Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 1 0 0 02. Amblema plicata threeridge 0 0 0 13. Fusconaia flava Wabash pigtoe 0 0 1 34. Elliptio dilatata spike 1 0 2 25. Leptodea fragilis paper pondshell 3 2 0 06. Ligumia recta black sandshell 0 0 0 17. Lampsilis r. luteola fat mucket 3 0 0 08. Lampsilis cardium plain pocketbook 0 1 0 0Total number of individual 8 3 3 7Total individual 1) live + dead, 2) total 11 21H’ 0.5936 0.7088Jaccard coefficient of similarity (% similar – total & extant communities) 78% Extant species 63Mussel-IBI* 22* quadrat sampling not done so % invasive bivavles given value of 157

Site 8: Big Walnut Creek (R.M. 22.3) at Big Walnut Park, at 39 o 56’57.0”N, 82 o 51’16.3”W,39.949177 o N -82.854546 o W, Franklin Co., Ohio, on 30 September 2012.Live mussels: Species Common name Length General Transect Age1. Strophitus undulatus creeper 46 x 22. Strophitus undulatus creeper 63 x 63. Lasmigona costata fluted-shell 109 x 144. Amblema plicata threeridge 94 x 105. Amblema plicata threeridge 128 x 156. Quadrula quadrula mapleleaf 70 x 107. Elliptio dilatata spike 68 x 88. Elliptio dilatata spike 77 x 129. Leptodea fragilis fragile papershell 96 x 810. Lampsilis r. luteola ♂ fat mucket 71 x 511. Lampsilis r. luteola ♂ fat mucket 93 x 1412. Lampsilis cardium ♂ plain pocketbook 115 x 1213. Lampsilis cardium ♀ plain pocketbook 115 x 1214. Lampsilis cardium ♂ plain pocketbook 126 x 2215. Lampsilis fasciola ♀ wavy-rayed pocketbook 80 x 616. Lampsilis fasciola ♀ wavy-rayed pocketbook 80 x 617. Lampsilis fasciola ♀ wavy-rayed pocketbook 95 x 818. Ptychobranchus fasciolaris kidneyshell 20 T1Q4 119. Fusconaia flava Wabash pigtoe 36 T1Q7 420. Fusconaia flava Wabash pigtoe 75 T1Q8 921. Leptodea fragilis fragile papershell 102 T1Q8 1222. Lampsilis r. luteola ♀ fatmucket 58 T1Q8 423. Lampsilis r. luteola ♂ fatmucket 86 T1Q9 724. Ptychobranchus fasciolaris kidneyshell 68 T1Q10 425. Lampsilis r. luteola ♀ fatmucket 64 T1Q10 426. Amblema plicata threeridge 95 T1Q11 1627. Fusconaia flava Wabash pigtoe 75 T1Q14 1028. Lampsilis r. luteola ♂ fatmucket 64 T1Q15 529. Ptychobranchus fasciolaris kidneyshell 27 T1Q18 230. Ptychobranchus fasciolaris kidneyshell 27 T1Q18 231. Lampsilis cardium ♂ plain pocketbook 64 T1Q18 332. Ptychobranchus fasciolaris kidneyshell 27 T1Q19 233. Tritogonia verrucosa pistolgrip 97 T1Q19 634. Elliptio dilatata spike 40 T2Q1 235. Alasmidonta marginata elktoe 77 T2Q2 1136. Fusconaia flava Wabash pigtoe 75 T2Q2 1337. Strophitus undulatus creeper 61 T2Q3 538. Fusconaia flava Wabash pigtoe 37 T2Q3 339. Lampsilis cardium ♂ plainpocketbook 58 T2Q3 240. Fusconaia flava Wabash pigtoe 25 T2Q4 241. Lampsilis r. luteola ♀ fat mucket 77 T2Q4 758

42. Alasmidonta marginata elktoe 47 T2Q5 243. Fusconaia flava Wabash pigtoe 27 T2Q5 144. Fusconaia flava Wabash pigtoe 66 T2Q5 745. Lampsilis r. luteola ♂ fatmucket 62 T2Q5 546. Lampsilis cardium juv plainpocketbook 50 T2Q5 247. Alasmidonta marginata elktoe 36 T2Q6 248. Lasmigona costata fluted-shell 74 T2Q6 449. Truncilla truncata deertoe 37 T2Q6 350. Lampsilis r. luteola ♀ fat mucket 52 T2Q7 351. Lampsilis r. luteola juv fat mucket 20 T2Q8 152. Fusconaia flava Wabash pigtoe 32 T2Q9 353. Fusconaia flava Wabash pigtoe 55 T2Q9 754. Leptodea fragilis fragile papershell 106 T2Q9 1055. Lampsilis r. luteola juv fat mucket 22 T2Q9 156. Lampsilis r. luteola ♂ fatmucket 47 T2Q9 357. Elliptio dilatata spike 26 T2Q11 158. Tritogonia verrucosa pistolgrip 115 T2Q12 1159. Fusconaia flava Wabash pigtoe 71 T2Q12 360. Ptychobranchus fasciolaris kidneyshell 58 T2Q12 361. Elliptio dilatata spike 90 T2Q13 1262. Lampsilis cardium ♀ plain pocketbook 73 T2Q13 363. Alasmidonta marginata elktoe 61 T2Q14 564. Fusconaia flava Wabash pigtoe 47 T2Q14 565. Fusconaia flava Wabash pigtoe 65 T2Q14 966. Leptodea fragilis fragile papershell 82 T2Q14 567. Leptodea fragilis fragile papershell 34 T2Q16 168. Lampsilis r. luteola ♂ fatmucket 64 T2Q16 369. Strophitus undulatus creeper 43 T2Q17 370. Tritogonia verrucosa pistolgrip 103 T2Q17 1871. Elliptio dilatata spike 51 T2Q17 572. Lampsilis r. luteola juv fat mucket 27 T2Q17 173. Fusconaia flava Wabash pigtoe 65 T2Q18 874. Elliptio dilatata spike 30 T2Q18 275. Truncilla truncata deertoe 24 T2Q18 276. Lampsilis r. luteola juv fat mucket 21 T2Q18 177. Lampsilis cardium ♂ plainpocketbook 111 T2Q19 978. Lampsilis r. luteola juv fat mucket 25 T2Q20 159

Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona costata fluted-shell 1 0 0 12. Amblema plicata threeridge 0 4 0 43. Fusconaia flava Wabash pigtoe 0 1 0 14. Elliptio dilatata spike 0 2 0 25. Ptychobranchus fasciolaris kidneyshell 0 1 0 16. Leptodea fragilis fragile papershell 13 0 0 137. Potamilus alatus pink heelsplitter 2 0 0 28. Lampsilis r. luteola fat mucket 0 0 2 29. Lampsilis cardium plain pocketbook 0 0 1 1Total 16 8 3 27Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 29/0 Q2: 26/0 Q3: 47/0 Q4: 50/1Q5: 34/0 Q6: 56/0 Q7: 35/1 Q8: 28/3Q9: 25/1 Q10: 18/2 Q11: 15/1 Q12: 24/0Q13: 32/0 Q14: 22/1 Q15: 34/1 Q16: 28/0Q17: 37/0 Q18: 48/3 Q19: 51/2 Q20: 43/0Total # of C. fluminea = 682: Density = 136.4/m 2Total # of mussels = 16: Density = 3.2/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 25/1 Q2: 28/2 Q3: 20/3 Q4: 20/2Q5: 22/5 Q6: 17/3 Q7: 15/1 Q8: 21/1Q9: 12/5 Q10: 15/0 Q11: 19/1 Q12: 13/3Q13: 10/2 Q14: 25/4 Q15: 29/0 Q16: 19/2Q17: 31/4 Q18: 36/4 Q19: 30/1 Q20: 21/1Total # of C. fluminea = 428: Density = 85.6/m 2Total # of mussels = 45: Density = 9.0/m 260

Summary Site Data Common name Live Dead Weathered Subfossil1. Strophitus undulatus creeper 4 0 0 02. Lasmigona costata fluted-shell 2 1 0 03. Alasmidonta marginata elktoe 4 0 0 04. Amblema plicata threeridge 3 0 4 05. Quadrula quadrula mapleleaf 1 0 0 06. Tritogonia verrucosa pistolgrip 2 0 0 07. Fusconaia flava Wabash pigtoe 14 0 1 08. Elliptio dilatata spike 7 0 2 09. Ptychobranchus fasciolaris kidneyshell 6 0 1 010. Leptodea fragilis fragile papershell 5 13 0 011. Potamilus alatus pink heelsplitter 0 2 0 012. Truncilla truncata deertoe 2 0 0 013. Lampsilis r. luteola fat mucket 16 0 0 214. Lampsilis cardium plain pocketbook 8 0 0 115. Lampsilis fasciola wavy-rayed pocket. 3 0 0 0Total number of individual 77 16 8 3Total individual 1) live + dead, 2) total 93 104H’ 1.0369 1.0428Jaccard coefficient of similarity (% similar – total & extant communities) 100% Extant species 100Mussel-IBI 3661

Site 9: Big Walnut Creek (R.M. 19.3) at Nerfzgel Park, at 39 o 54’47.3”N, 82 o 52’14.0”W,39.912873 o N -82.870410 o W, Franklin Co., Ohio, on 4 October 2012.Live mussels: Species Common name Length General Transect Age1. Lasmigona costata fluted-shell 102 x 92. Tritogonia verrucosa pistolgrip 112 x 103. Fusconaia flava Wabash pigtoe 76 x 124. Ptychobranchus fasciolaris kidneyshell 121 x 255. Lampsilis fasciola ♀ wavy-rayed pocketbook 52 x 6Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona costata fluted-shell 0 0 1 12. Lasmigona complanata white heelsplitter 0 0 2 23. Amblema plicata threeridge 0 1 2 34. Quadrula quadrula mapleleaf 0 0 1 15. Fusconaia flava Wabash pigtoe 0 1 0 16. Elliptio dilatata spike 0 3 6 97. Pleurobema sintoxia round pigtoe 0 1 0 18. Ptychobranchus fasciolaris kidneyshell 0 2 0 29. Leptodea fragilis fragile papershell 1 1 0 210. Lampsilis r. luteola fatmucket 0 0 4 4Total 1 9 16 26Transect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 262

Summary Site Data Common name Live Dead Weathered Subfossil1. Lasmigona costata fluted-shell 1 0 0 12. Lasmigona complanata white heelsplitter 0 0 0 23. Amblema plicata threeridge 0 0 1 24. Tritogonia verrucosa pistolgrip 1 0 0 05. Quadrula quadrula mapleleaf 0 0 0 16. Fusconaia flava Wabash pigtoe 1 0 1 07. Elliptio dilatata spike 0 0 3 68. Pleurobema sintoxia round pigtoe 0 0 1 09. Ptychobranchus fasciolaris kidneyshell 1 0 2 010. Leptodea fragilis paper pondshell 0 1 1 011. Lampsilis r. luteola fat mucket 0 0 0 412. Lampsilis fasciola wavy-rayed pocket. 1 0 0 0Total number of individual 5 1 9 16Total individual 1) live + dead, 2) total 6 31H’ 0.7782 0.9666Jaccard coefficient of similarity (% similar – total & extant communities) 67% Extant species 50Mussel-IBI* 26* quadrat sampling not done so % invasive bivavles given value of 163

Site 10: Big Walnut Creek (R.M. 18.0) downstream of Hamilton Road (SR 317), at39 o 54’36.4”N, 82 o 52’57.4”W, 39.910023 o N -82.883249 o W, Franklin Co., Ohio, on 4 October2012.Live mussels: Species Common name Length General Transect Age1. Pyganodon grandis giant floater 107 x 82. Leptodea fragilis fragile papershell 90 x 83. Potamilus alatus pink heelsplitter 104 x 94. Lampsilis r. luteola ♂ fatmucket 68 x 55. Lampsilis cardium ♂ plain pocketbook 91 x 86. Lampsilis cardium ♀ plain pocketbook 100 x ---7. Lampsilis cardium ♀ plain pocketbook 110 x ---8. Lampsilis cardium ♀ plain pocketbook 125 x ---Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona complanata white heelsplitter 0 0 3 32. Amblema plicata threeridge 0 0 1 13. Quadrula quadrula mapleleaf 0 0 1 14. Fusconaia flava Wabash pigtoe 0 0 1 15. Elliptio dilatata spike 0 0 6 66. Ptychobranchus fasciolaris kidneyshell 0 0 1 17. Leptodea fragilis fragile papershell 0 1 0 18. Obovaria subrotunda hickorynut 0 0 1 19. Truncilla donaciformis fawnfoot 0 0 1 110. Lampsilis r. luteola fatmucket 0 1 5 611. Lampsilis fasciola wavy-rayed pocketbook 0 0 1 1Total 0 2 21 23Transect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 264

Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 1 0 0 02. Lasmigona complanata white heelsplitter 0 0 0 33. Amblema plicata threeridge 0 0 0 14. Quadrula quadrula mapleleaf 0 0 0 15. Fusconaia flava Wabash pigtoe 0 0 0 16. Elliptio dilatata spike 0 0 0 67. Ptychobranchus fasciolaris kidneyshell 0 0 0 18. Leptodea fragilis paper pondshell 1 0 1 09. Potamilius alatus pink heelsplitter 1 0 0 010. Obovaria subrotunda hickorynut 0 0 0 111. Truncilla donaciformis fawnfoot 0 0 0 112. Lampsilis r. luteola fat mucket 1 0 1 513. Lampsilis cardium plain pocketbook 4 0 0 014. Lampsilis fasciola wavy-rayed pocket. 0 0 0 1Total number of individual 8 0 2 21Total individual 1) live + dead, 2) total 8 31H’ 0.6021 1.0066Jaccard coefficient of similarity (% similar – total & extant communities) 61% Extant species 36Mussel-IBI* 16* quadrat sampling not done so % invasive bivavles given value of 165

Site 11: Big Walnut Creek (R.M. 15.8) at East Williams Road, at 39 o 53’15.6”N, 82 o 54’18.4”W,39.887874 o N -82.905006 o W, Franklin Co., Ohio, on 11 October 2012.Live mussels: Species Common name Length General Transect Age1. Tritogonia verrucosa pistolgrip 129 x 142. Potamilus alatus pink heelsplitter 112 x 103. Lampsilis r. luteola ♂ fatmucket 62 x 54. Lampsilis r. luteola ♂ fatmucket 71 x 85. Lampsilis r. luteola ♂ fatmucket 72 x 76. Lampsilis r. luteola ♂ fatmucket 79 x 7Dead mussels: Species Common name Dead Weathered Subfossil Total1. Pyganodon grandis giant floater 0 1 0 12. Lasmigona costata fluted-shell 0 0 1 13. Lasmigona complanata white heelsplitter 0 0 1 14. Amblema plicata threeridge 0 0 1 15. Quadrula quadrula mapleleaf 0 0 1 16. Fusconaia flava Wabash pigtoe 0 0 2 27. Elliptio dilatata spike 0 0 3 38. Ptychobranchus fasciolaris kidneyshell 0 0 2 29. Leptodea fragilis paper pondshell 0 1 0 110. Obovaria subrotunda hickorynut 0 0 1 1Total 0 2 12 14Transect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 266

Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 0 0 1 02. Lasmigona costata fluted-shell 0 0 0 13. Lasmigona complanata white heelsplitter 0 0 0 14. Amblema plicata threeridge 0 0 0 15. Tritogonia verrucosa pistolgrip 1 0 0 06. Quadrula quadrula mapleleaf 0 0 0 17. Fusconaia flava Wabash pigtoe 0 0 0 28. Elliptio dilatata spike 0 0 0 39. Ptychobranchus fasciolaris kidneyshell 0 0 0 210. Leptodea fragilis paper pondshell 0 0 1 011. Potamilius alatus pink heelsplitter 1 0 0 012. Obovaria subrotunda hickorynut 0 0 0 113. Lampsilis r. luteola fat mucket 4 0 0 0Total number of individual 6 0 2 12Total individual 1) live + dead, 2) total 6 20H’ 0.3768 1.0488Jaccard coefficient of similarity (% similar – total & extant communities) 57% Extant species 23Mussel-IBI* 16* quadrat sampling not done so % invasive bivavles given value of 167

Site 12: Big Walnut Creek (R.M. 14.8) downstream of three rivers MetroPark, at 39 o 52’24.5”N,82 o 54’43.6”W, 39.873349 o N -82.912382 o W, Franklin Co., Ohio, on 11 October 2012.Live mussels: Species Common name Length General Transect Age1. Tritogonia verrucosa pistolgrip 68 x 52. Potamilus alatus pink heelsplitter 121 x 183. Potamilus alatus pink heelsplitter 129 x 204. Leptodea fragilis fragile papershell 60 x 45. Leptodea fragilis fragile papershell 102 x 10Dead mussels: Species Common name Dead Weathered Subfossil TotalNone collectedTransect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Tritogonia verrucosa pistolgrip 1 0 0 02. Leptodea fragilis paper pondshell 2 0 0 03. Potamilius alatus pink heelsplitter 2 0 0 0Total number of individual 5 0 0 0Total individual 1) live + dead, 2) total 5 5H’ 0.4582 0.4582Jaccard coefficient of similarity (% similar – total & extant communities) ------% Extant species 100Mussel-IBI ------68

Site 13: Big Walnut Creek (R.M. 8.4) upstream of London-Groveport Bridge (SR 317), at39 o 50’00.5”N, 82 o 59’30.2”W, 39.833993 o N -82.980494 o W, Franklin Co., Ohio, on 13September 2012.Live mussels: Species Common name Length General Transect Age1. Leptodea fragilis fragile papershell 99 x 142. Potamilus alatus pink heelsplitter --- x ---3. Lampsilis cardium ♀ plain pocketbook --- x ---4. Lampsilis cardium ♂ plain pocketbook 99 T1Q7 65. Potamilus alatus pink heelsplitter 100 T2Q5 86. Amblema plicata threeridge 115 T2Q6 207. Truncilla truncata deertoe 36 T2Q6 38. Quadrula pustulosa pimpleback 62 T2Q7 129. Potamilus alatus pink heelsplitter 95 T2Q7 1410. Truncilla truncata deertoe 52 T2Q11 511. Amblema plicata threeridge 96 T2Q13 912. Lampsilis cardium juv plain pocketbook 35 T2Q14 1Dead mussels: Species Common name Dead Weathered Subfossil Total1. Amblema plicata threeridge 1 1 0 22. Quadrula quadrula mapleleaf 0 0 1 13. Quadrula pustulosa pimpleback 0 1 0 14. Fusconaia flava Wabash pigtoe 0 1 0 15. Leptodea fragilis fragile papershell 12 1 0 136. Potamilus alatus pink heelsplitter 30 0 0 307. Potamilus ohiensis pink papershell 1 0 0 18. Lampsilis cardium plain pocketbook 1 0 0 1Total 45 4 1 50Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 2/0 Q2: 6/0 Q3: 5/0 Q4: 4/0Q5: 6/0 Q6: 12/0 Q7: 20/1 Q8: 28/0Q9: 12/0 Q10: 10/0 Q11: 15/0 Q12:0 30/0Q13: 17/0 Q14: 20/0 Q15: 20/0 Q16: 25/0Q17: 15/0 Q18: 20/0 Q19: 10/0 Q20: 10/0Total # of C. fluminea = 287: Density = 57.4 /m 2Total # of mussels = 1: Density = 0.2/m 269

Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 8/0 Q2: 6/0 Q3: 9/0 Q4: 12/0Q5: 15/1 Q6: 10/2 Q7: 11/2 Q8: 23/0Q9: 21/0 Q10: 22/0 Q11: 22/1 Q12: 17/0Q13: 15/1 Q14: 12/1 Q15: 22/0 Q16: 19/0Q17: 35/0 Q18: 21/0 Q19: 21/0 Q20: 11/0Total # of C. fluminea = 332: Density = 66.4/m 2Total # of mussels = 8: Density = 1.6/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Amblema plicata threeridge 2 1 1 02. Quadrula quadrula mapleleaf 0 0 0 13. Quadrula pustulosa pimpleback 1 0 1 04. Fusconaia flava Wabash pigtoe 0 0 1 05. Leptodea fragilis fragile papershell 1 12 1 06. Potamilus alatus pink heelsplitter 3 30 0 07. Potamilus ohiensis pink papershell 0 1 0 08. Truncilla truncata deertoe 2 0 0 09. Lampsilis cardium plain pocketbook 3 1 0 0Total number of individual 12 45 4 1Total individual 1) live + dead, 2) total 57 62H’ 0.5448 0.6282Jaccard coefficient of similarity (% similar – total & extant communities) 82% Extant species 78Mussel-IBI 3470

Site 14: Big Walnut Creek (R.M. 6.9) downstream of London-Groveport Bridge (SR 317), at39 o 49’52.9”N, 82 o 59’33.6”W, 39.831382 o N -82.992654 o W, Franklin Co., Ohio, 13 September 2012.Live mussels: Species Common name Length General Transect Age1. Lampsilis cardium juv plain pocketbook 46 T1Q1 22. Tritogonia verrucosa pistolgrip 120 T1Q7 123. Lampsilis cardium ♂ plain pocketbook 100 T1Q14 204. Lampsilis r. luteola juv fatmucket 28 T2Q1 25. Potamilus alatus pink heelsplitter 65 T2Q6 36. Leptodea fragilis fragile papershell 42 T2Q6 37. Potamilus alatus pink heelsplitter 55 T2Q7 28. Potamilus alatus pink heelsplitter 67 T2Q14 4Dead mussels: Species Common name Dead Weathered Subfossil Total1. Amblema plicata threeridge 0 2 0 22. Tritogonia verrucosa pistolgrip 0 3 0 33. Quadrula quadrula mapleleaf 0 0 1 14. Leptodea fragilis fragile papershell 6 0 0 65. Potamilus alatus pink heelsplitter 10 0 0 106. Truncilla truncata deertoe 0 1 0 17. Ligumia recta black sandshell 1 0 0 1Total 17 6 1 24Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 15/1 Q2: 20/0 Q3: 20/0 Q4: 10/0Q5: 10/0 Q6: 20/0 Q7: 10/1 Q8: 10/0Q9: 10/0 Q10: 10/0 Q11: 5/0 Q12: 10/0Q13: 10/0 Q14: 10/1 Q15: 10/0 Q16: 15/0Q17: 20/0 Q18: 15/0 Q19: 15/0 Q20: 10/0Total # of C. fluminea = 255: Density = 51.0/m 2Total # of mussels = 3: Density = 0.6/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 7/1 Q2: 6/0 Q3: 4/0 Q4: 21/0Q5: 20/0 Q6: 32/2 Q7: 19/1 Q8: 10/0Q9: 23/0 Q10: 21/0 Q11: 15/0 Q12: 31/0Q13: 37/0 Q14: 21/1 Q15: 37/0 Q16: 21/0Q17: 9/0 Q18: 40/0 Q19: 31/0 Q20: 29/0Total # of C. fluminea = 434: Density = 86.8/m 2Total # of mussels = 5: Density = 1.0/m 271

Summary Site Data Common name Live Dead Weathered Subfossil1. Amblema plicata threeridge 0 0 2 02. Tritogonia verrucosa pistolgrip 1 0 3 03. Quadrula quadrula mapleleaf 0 0 1 04. Leptodea fragilis fragile papershell 1 6 0 05. Potamilus alatus pink heelsplitter 3 10 0 06. Truncilla truncata deertoe 0 0 1 07. Ligumia recta black sandshell 0 1 0 08. Lampsilis r. luteola fatmucket 1 0 0 09. Lampsilis cardium plain pocketbook 2 0 0 0Total number of individual 8 17 7 0Total individual 1) live + dead, 2) total 25 32H’ 0.5579 0.7549Jaccard coefficient of similarity (% similar – total & extant communities) 75% Extant species 67Mussel-IBI 2872

Site 15: Big Walnut Creek (R.M. 6.0) adjacent to gravel quarry, at 39 o 49’25.3”N, 82 o 59’23.1”W,39.823557 o N -82.990004 o W, Franklin Co., Ohio, on 15 September 2012.Live mussels: Species Common name Length General Transect Age1. Tritogonia verrucosa pistolgrip 133 x 202. Potamilus alatus pink heelsplitter 51 x 23. Potamilus alatus pink heelsplitter 59 x 34. Leptodea fragilis fragile papershell 46 x 25. Lampsilis cardium plain pocketbook --- x ---Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona complanata white heelsplitter 0 1 0 12. Ptychobranchus fasciolaris kidneyshell 0 1 0 13. Leptodea fragilis paper pondshell 5 0 0 54. Potamilius alatus pink heelsplitter 8 0 0 8Total 13 2 0 15Transect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Lasmigona complanata white heelsplitter 0 0 1 02. Tritogonia verrucosa pistolgrip 1 0 0 03. Ptychobranchus fasciolaris kidneyshell 0 0 1 04. Leptodea fragilis paper pondshell 1 5 0 05. Potamilius alatus pink heelsplitter 2 8 0 06. Lampsilis cardium plain pocketbook 1 0 0 0Total number of individual 5 13 2 0Total individual 1) live + dead, 2) total 18 20H’ 0.4403 0.5676Jaccard (only for sites with 10 species are more) ------% Extant species 67Mussel-IBI (only for site with 8 species are more) ------73

Site 16: Big Walnut Creek (R.M. 4.5) off of Lockbourne Road, at 39 o 58’11.3”N, 82 o 58’11.3”W,39.819118 o N -82.970161 o W, Franklin Co., Ohio, on 16 September 2012.Live mussels: Species Common name Length General Transect Age1. Pyganodon grandis giant floater 130 x 122. Pyganodon grandis giant floater 130 x 153. Pyganodon grandis giant floater 139 x 174. Pyganodon grandis giant floater 160 x 235. Lasmigona complanata white heelsplitter 128 x 76. Amblema plicata threeridge 50 x 37. Amblema plicata threeridge 106 x 128. Tritogonia verrucosa pistolgrip --- x ---9. Tritogonia verrucosa pistolgrip 128 x 1810. Tritogonia verrucosa pistolgrip 132 x 2011. Tritogonia verrucosa pistolgrip 132 x 2012. Tritogonia verrucosa pistolgrip 135 x 2013. Tritogonia verrucosa pistolgrip 135 x 2014. Tritogonia verrucosa pistolgrip 139 x 1815. Megalonaias nervosa washboard 210 x 3516. Potamilus alatus pink heelsplitter 65 x 417. Obovaria subrotunda hickorynut --- x ---18. Ligumia recta black sandshell --- x ---19. Ligumia recta ♀ black sandshell 131 x 920. Ligumia recta ♂ black sandshell 160 x 1621. Lampsilis r. luteola ♀ fatmucket 60 x 322. Lampsilis cardium ♂ plain pocketbook 92 x 6Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona complanata white heelsplitter 0 1 0 12. Lasmigona costata fluted-shell 0 1 0 13. Amblema plicata threeridge 0 3 1 44. Tritogonia verrucosa pistolgrip 1 2 1 45. Quadrula cylindrica rabbitsfoot 0 0 1 16. Quadrula pustulosa pimpleback 0 2 0 27. Fusconaia flava Wabash pigtoe 0 1 0 18. Elliptio dilatata spike 0 2 0 29. Pleurobema clava clubshell 0 0 1 110. Pleurobema sintoxia round pigtoe 0 1 1 211. Cyclonaias tuberculata purple wartyback 0 0 2 212. Megalonaias nervosa washboard 0 2 0 213. Ptychobranchus fasciolaris kidneyshell 0 2 1 314. Leptodea fragilis fragile papershell 13 0 0 1315. Potamilus alatus pink heelsplitter 15 0 0 1516. Potamilus ohiensis pink papershell 1 0 0 174

17. Lampsilis r. luteola fatmucket 0 1 0 118. Lampsilis cardium plain pocketbook 0 1 0 119. Lampsilis fasciola wavy-rayed pocketbook 1 0 0 120. Epioblasma triquetra snuffbox 0 0 1 1Total 31 19 9 59Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 21/0 Q2: 23/0 Q3: 21/0 Q4: 22/0Q5: 19/0 Q6: 5/0 Q7: 3/0 Q8: 10/0Q9: 11/0 Q10: 3/0 Q11: 10/0 Q12: 12/0Q13: 3/0 Q14: 7/0 Q15: 5/0 Q16: 10/0Q17: 11/0 Q18: 3/0 Q19: 5/0 Q20: 2/0Total # of C. fluminea = 206: Density = 41.2/m 2Total # of mussels = 0 Density = 0.0/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 10/0 Q2: 15/0 Q3: 10/0 Q4: 20/0Q5: 15/0 Q6: 15/0 Q7: 10/0 Q8: 5/0Q9: 20/0 Q10: 10/0 Q11: 10/0 Q12: 10/0Q13: 5/0 Q14: 10/0 Q15: 10/0 Q16: 10/0Q17: 10/0 Q18: 10/0 Q19: 10/0 Q20: 10/0Total # of C. fluminea = 225: Density = 45.0/m 2Total # of mussels = 0: Density = 0.0/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 4 0 0 02. Lasmigona complanata white heelsplitter 1 0 1 03. Lasmigona costata fluted-shell 0 0 1 04. Amblema plicata threeridge 2 0 3 15. Tritogonia verrucosa pistolgrip 8 1 2 16. Quadrula cylindrica rabbitsfoot 0 0 0 17. Quadrula pustulosa pimpleback 0 0 2 08. Fusconaia flava Wabash pigtoe 0 0 1 09. Elliptio dilatata spike 0 0 2 010. Pleurobema clava clubshell 0 0 0 111. Pleurobema sintoxia round pigtoe 0 0 1 112. Cyclonaias tuberculata purple wartyback 0 0 0 213. Megalonaias nervosa washboard 1 0 2 014. Ptychobranchus fasciolaris kidneyshell 0 0 2 115. Leptodea fragilis fragile papershell 0 13 0 016. Potamilus alatus pink heelsplitter 1 15 0 075

17. Potamilus ohiensis pink papershell 0 1 0 018. Obovaria subrotunda hickorynut 1 0 0 019. Ligumia recta black sandshell 3 0 0 020. Lampsilis r. luteola fatmucket 1 0 1 021. Lampsilis cardium plain pocketbook 0 0 1 022. Lampsilis fasciola wavy-rayed pocket. 0 1 0 023. Epioblasma triquetra snuffbox 0 0 0 1Total number of individual 22 31 19 9Total individual 1) live + dead, 2) total 53 81H’ 0.4326 1.1468Jaccard coefficient of similarity (% similar – total & extant communities) 68% Extant species 52Mussel-IBI 3276

Site 17: Big Walnut Creek (R.M. 4.1) upstream of Rowe Road Bridge (CR 133), at39 o 48’42.8”N, 82 o 58’11.3”W, 39.812071 o N -82.969446 o W, Franklin Co., Ohio, on 16September 2012.Live mussels: Species Common name Length General Transect Age1. Pyganodon grandis giant floater 91 x 62. Pyganodon grandis giant floater 109 x 83. Amblema plicata threeridge 94 x 144. Amblema plicata threeridge 99 x 95. Amblema plicata threeridge 110 x 126. Tritogonia verrucosa pistolgrip 103 x 97. Tritogonia verrucosa pistolgrip 119 x 178. Tritogonia verrucosa pistolgrip 123 x 119. Tritogonia verrucosa pistolgrip 138 x 2210. Tritogonia verrucosa pistolgrip 150 x 1711. Leptodea fragilis fragile papershell 54 x 312. Potamilus alatus pink heelsplitter 106 x 813. Ligumia recta ♂ black sandshell 154 x 1414. Lampsilis cardium ♀ plain pocketbook --- x ---15. Lampsilis fasciola ♀ wavy-rayed pocketbook 73 x 16Dead mussels: Species Common name Dead Weathered Subfossil Total1. Amblema plicata threeridge 0 1 0 12. Quadrula pustulosa pimpleback 0 1 0 13. Elliptio dilatata spike 0 0 1 14. Pleurobema sintoxia round pigtoe 0 1 0 15. Cyclonaias tuberculata purple wartyback 0 0 1 16. Megalonaias nervosa washboard 0 1 0 17. Leptodea fragilis fragile papershell 2 0 0 28. Potamilus alatus pink heelsplitter 4 0 0 49. Lampsilis fasciola wavy-rayed pocketbook 1 0 0 110. Epioblasma t. rangiana northern riffleshell 0 0 1 1Total 7 4 3 14Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 7/0 Q2: 12/0 Q3: 4/0 Q4: 15/0Q5: 17/0 Q6: 10/0 Q7: 10/0 Q8: 20/0Q9: 21/0 Q10: 36/0 Q11: 22/0 Q12: 33/0Q13: 31/0 Q14: 18/0 Q15: 26/0 Q16: 12/0Q17: 13/0 Q19: 3/0 Q19: 22/0 Q20: 15/0Total # of C. fluminea = 347: Density = 69.4/m 2Total # of mussels = 0 Density = 0.0/m 277

Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 3/0 Q2: 2/0 Q3: 4/0 Q4: 0/0Q5: 5/0 Q6: 10/0 Q7: 10/0 Q8: 10/0Q9: 10/0 Q10: 14/0 Q11: 10/0 Q12: 10/0Q13: 10/0 Q14: 10/0 Q15: 10/0 Q16: 10/0Q17: 10/0 Q18: 10/0 Q19: 10/0 Q20: 10/0Total # of C. fluminea = 168: Density = 33.6/m 2Total # of mussels = 0: Density = 0.0/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 2 0 0 02. Amblema plicata threeridge 3 0 1 03. Tritogonia verrucosa pistolgrip 5 0 0 04. Quadrula pustulosa pimpleback 0 0 1 05. Elliptio dilatata spike 0 0 0 16. Pleurobema sintoxia round pigtoe 0 0 1 07. Cyclonaias tuberculata purple wartyback 0 0 0 18. Megalonaias nervosa washboard 0 0 1 09. Leptodea fragilis fragile papershell 1 2 0 010. Potamilus alatus pink heelsplitter 1 4 0 011. Ligumia recta black sandshell 1 0 0 012. Lampsilis cardium plain pocketbook 1 0 0 013. Lampsilis fasciola wavy-rayed pocket. 1 1 0 014. Epioblasma t. rangiana northern riffleshell 0 0 0 1Total number of individual 15 7 4 3Total individual 1) live + dead, 2) total 22 29H’ 0.8537 1.0487Jaccard coefficient of similarity (% similar – total & extant communities) 70% Extant species 57Mussel-IBI 2478

Site 18: Big Walnut Creek (R.M. 3.5) downstream of Rowe Road Bridge (CR 133), at 39 o 48’34.6”N,82 o 58’38.8”W, 39.809502 o N -82.977974 o W, Franklin Co., Ohio, on 13 October 2012.Live mussels: Species Common name Length General Transect AgeNone found aliveDead mussels: Species Common name Dead Weathered Subfossil Total1. Tritogonia verrucosa pistolgrip 0 1 0 12. Leptodea fragilis fragile papershell 0 5 0 53. Potamilus alatus pink heelsplitter 0 11 0 11Total 0 17 0 17Transect data: Transect 1 – # of Corbicula fluminea/musselsQ1: 15/0 Q2: 12/0 Q3: 10/0 Q4: 7/0Q5: 5/0 Q6: 0/0 Q7: 13/0 Q8: 20/0Q9: 16/0 Q10: 5/0 Q11: --/- Q12: --/-Q13: --/- Q14: --/- Q15: --/- Q16: --/-Q17: --/- Q19: --/- Q19: --/- Q20: --/-Total # of C. fluminea = 206: Density = 41.2/m 2Total # of mussels = 0 Density = 0.0/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsQ1: 21/0 Q2: 17/0 Q3: 15/0 Q4: 5/0Q5: 6/0 Q6: 3/0 Q7: 2/0 Q8: 10/0Q9: 11/0 Q10: 10/0 Q11: --/- Q12: --/-Q13: --/- Q14: --/- Q15: --/- Q16: --/-Q17: --/- Q18: --/- Q19: --/- Q20: --/-Total # of C. fluminea = 200: Density = 40.0/m 2Total # of mussels = 0: Density = 0.0/m 2Summary Site Data Common name Live Dead Weathered Subfossil1. Tritogonia verrucosa pistolgrip 0 0 1 02. Leptodea fragilis fragile papershell 0 0 5 03. Potamilus alatus pink heelsplitter 0 0 11 0Total number of individual 0 0 17 0Total individual 1) live + dead, 2) total 0 17H’ 0.0 0.3510Jaccard coefficient of similarity (% similar – total & extant communities) ------% Extant species 0Mussel-IBI ------79

Site 19: Big Walnut Creek (R.M. 1.6) downstream of US Rt. 23 Bridge, at 39 o 47’59.5”N,83 o 00’14.9”W, 39.8806785 o N -82.996485 o W, Franklin Co., Ohio, on 20 September 2012.Live mussels: Species Common name Length General Transect Age1. Pyganodon grandis giant floater 102 x 92. Tritogonia verrucosa pistolgrip 106 x 73. Tritogonia verrucosa pistolgrip 122 x 124. Tritogonia verrucosa pistolgrip 126 x 125. Lampsilis cardium ♀ plain pocketbook --- x ---Dead mussels: Species Common name Dead Weathered Subfossil Total1. Alasmidonta marginata elktoe 0 1 0 12. Amblema plicata threereidge 0 1 0 13. Tritogonia verrucosa pistolgrip 3 0 0 34. Quadrula quadrula mapleleaf 0 2 0 25. Quadrula pustulosa pimpleback 0 1 0 16. Pleurobema sintoxia round pigtoe 0 0 1 17. Cyclonaias tuberculata purple wartyback 0 1 0 18. Ptychobranchus fasciolaris kidneyshell 0 0 1 19. Leptodea fragilis fragile papershell 9 0 0 910. Potamilus alatus pink heelsplitter 10 0 0 1011. Truncilla truncata deertoe 0 1 0 112. Lampsilis cardium plain pocketbook 1 0 0 1Total 24 7 2 33Transect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 280

Summary Site Data Common name Live Dead Weathered Subfossil1. Pyganodon grandis giant floater 1 0 0 02. Alasmidonta marginata elktoe 0 0 1 03. Amblema plicata threereidge 0 0 1 04. Tritogonia verrucosa pistolgrip 3 3 0 05. Quadrula quadrula mapleleaf 0 0 2 06. Quadrula pustulosa pimpleback 0 0 1 07. Pleurobema sintoxia round pigtoe 0 0 0 18. Cyclonaias tuberculata purple wartyback 0 0 1 09. Ptychobranchus fasciolaris kidneyshell 0 0 0 110. Leptodea fragilis fragile papershell 0 9 0 011. Potamilus alatus pink heelsplitter 0 10 0 012. Truncilla truncata deertoe 0 0 1 013. Lampsilis cardium plain pocketbook 1 1 0 0Total number of individual 5 24 7 2Total individual 1) live + dead, 2) total 29 38H’ 0.5951 0.9071Jaccard coefficient of similarity (% similar – total & extant communities) 62% Extant species 39Mussel-IBI* 20* quadrat sampling not done so % invasive bivavles given value of 181

Site 20: Big Walnut Creek (R.M. 0.7) further downstream of US Rt. 23 Bridge, at 39 o 47’59.5”N,83 o 00’14.9”W, 39.80012 o N -83.004117 o W, Pickaway Co., Ohio, on 20 September 2012.Live mussels: Species Common name Length General Transect Age1. Tritogonia verrucosa pistolgrip 98 x 72. Megalonaias nervosa washboard 170 x 203. Potamilus alatus pink heelsplitter 116 x 184. Potamilus alatus pink heelsplitter 117 x 75. Potamilus alatus pink heelsplitter 138 x 206. Lampsilis cardium ♀ plain pocketbook --- x ---7. Lampsilis cardium ♀ plain pocketbook --- x ---8. Lampsilis fasciola ♀ wavy-rayed pocketbook --- x ---Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona costata fluted shell 0 0 1 12. Tritogonia verrucosa pistolgrip 1 2 0 33. Quadrula pustulosa pimpleback 1 2 0 34. Ptychobranchus fasciolaris kidneyshell 0 1 0 15. Leptodea fragilis fragile papershell 11 0 0 116. Potamilus alatus pink heelsplitter 9 0 0 97. Truncilla truncata deertoe 1 2 0 38. Villosa iris rainbow 1 0 0 19. Ligumia recta black sandshell 0 1 0 110. Lampsilis r. luteola fatmucket 0 1 0 111. Lampsilis cardium plain pocketbook 1 0 0 112. Lampsilis fasciola wavy-rayed pocketbook 1 1 0 213. Epioblasma triquetra snuffbox 0 0 1 1Total 26 10 2 38Transect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 282

Summary Site Data Common name Live Dead Weathered Subfossil1. Lasmigona costata fluted shell 0 0 0 12. Tritogonia verrucosa pistolgrip 1 1 2 03. Quadrula pustulosa pimpleback 0 1 2 04. Megalonaias nervosa washboard 1 0 0 05. Ptychobranchus fasciolaris kidneyshell 0 0 1 06. Leptodea fragilis fragile papershell 0 11 0 07. Potamilus alatus pink heelsplitter 3 9 0 08. Truncilla truncata deertoe 0 1 2 09. Villosa iris rainbow 0 1 0 010. Ligumia recta black sandshell 0 0 1 011. Lampsilis r. luteola fatmucket 0 0 1 012. Lampsilis cardium plain pocketbook 2 1 0 013. Lampsilis fasciola wavy-rayed pocket. 1 1 1 014. Epioblasma triquetra snuffbox 0 0 0 1Total number of individual 8 26 10 2Total individual 1) live + dead, 2) total 34 46H’ 0.7362 0.9554Jaccard coefficient of similarity (% similar – total & extant communities) 74% Extant species 64Mussel-IBI* 32* quadrat sampling not done so % invasive bivavles given value of 183

Site 21: Big Walnut Creek (R.M. 0.1) just upstream of its mouth, at 39 o 47’57.6”N,83 o 00’34.4”W, 39.799587 o N -83.007799 o W, Pickaway Co., Ohio, on 20 September 2012.Live mussels: Species Common name Length General Transect Age1. Amblema plicata threeridge 102 x 11Dead mussels: Species Common name Dead Weathered Subfossil Total1. Lasmigona costata fluted shell 0 1 0 12. Lasmigona complanata white heelsplitter 0 1 0 13. Amblema plicata threeridge 0 2 0 24. Quadrula quadrula mapleleaf 0 1 0 15. Quadrula pustulosa pimpleback 0 1 0 16. Leptodea fragilis fragile papershell 2 0 0 27. Potamilus alatus pink heelsplitter 5 0 0 58. Truncilla truncata deertoe 1 2 0 39. Truncilla donaciformis fawnfoot 0 0 1 110. Lampsilis cardium plain pocketbook 0 1 0 1Total 8 9 1 18Transect data: Transect 1 – # of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 2Transect data: Transect 2 –# of Corbicula fluminea/musselsNot doneTotal # of C. fluminea = ---: Density = ---/m 2Total # of mussels = ---: Density = ---/m 284

Summary Site Data Common name Live Dead Weathered Subfossil1. Lasmigona costata fluted shell 0 0 1 02. Lasmigona complanata white heelsplitter 0 0 1 03. Amblema plicata threeridge 1 0 2 04. Quadrula quadrula mapleleaf 0 0 1 05. Quadrula pustulosa pimpleback 0 0 1 06. Leptodea fragilis fragile papershell 0 2 0 07. Potamilus alatus pink heelsplitter 0 5 0 08. Truncilla truncata deertoe 0 1 2 09. Truncilla donaciformis fawnfoot 0 0 0 110. Lampsilis cardium plain pocketbook 0 0 1 0Total number of individual 1 8 9 1Total individual 1) live + dead, 2) total 9 19H’ 0.4990 0.9125Jaccard coefficient of similarity (% similar – total & extant communities) 63% Extant species 40Mussel-IBI* 22* quadrat sampling not done so % invasive bivavles given value of 185

Mussel-IBI calculations for the Big Walnut Creek project from Hoover Reservoir to its mouthSite 1: Big Walnut Creek downstream of Central College Road, at 40 o 05’54.5”N, 82 o 52’50.5”W,40.097607 o N -82.880414 o W, Franklin Co., Ohio, on 1 September 2012 and 2 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 1 x 32 % state listed species 13% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 56% x 35 % extant individuals 63% x 56 ratio of mean to median age 0.92 x 3Subtotal 11/15 73%Community Structure7 Shannon-Weiner Index 0.6642 x 38 % invasive bivalves 99% x 19 % burying species 50% x 310 total # of extant individuals 31 x 3Subtotal 10/20 50%Total Score 5 21 2 28______________________________________________________________________________86

Site 2: Big Walnut Creek downstream of Morse Road, at 40 o 03’40.1”N, 82 o 53’27.3”W,40.051029 o N -82.890739 o W, Franklin Co., Ohio, on 9 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 1 x 32 % state listed species 9% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 55% x 35 % extant individuals 52% x 36 ratio of mean to median age 0.91 x 3Subtotal 9/15 60%Community Structure7 Shannon-Weiner Index 0.5353 x 38 % invasive bivalves 78% x 19 % burying species 55% x 310 total # of extant individuals 24 x 1Subtotal 8/20 40%Total Score 0 21 3 24______________________________________________________________________________87

Site 3: Big Walnut Creek at Woodside Green Park, at 40 o 02’38.7”N, 82 o 52’47.9”W,40.044091 o N -82.879978 o W, Franklin Co., Ohio, on 22 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 3 x 32 % state listed species 25% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 83% x 55 % extant individuals 97% x 56 ratio of mean to median age 0.70 x 5Subtotal 15/15 100%Community Structure7 Shannon-Weiner Index 0.7310 x 38 % invasive bivalves 85% x 19 % burying species 67% x 310 total # of extant individuals 132 x 5Subtotal 12/20 60%Total Score 20 12 2 34______________________________________________________________________________88

Site 4: Big Walnut Creek adjacent to Gahanna Municipal Golf Course, at 40 o 01’47.3”N,-82 o 52’40.2”W, 40.029562 o N 82.877703 o W, Franklin Co., Ohio, on 29 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 2 x 32 % state listed species 13% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 73% x 55 % extant individuals 72% x 56 ratio of mean to median age 0.90 x 5Subtotal 15/15 100%Community Structure7 Shannon-Weiner Index 0.8975 x 58 % invasive bivalves 94% x 19 % burying species 63% x 310 total # of extant individuals 53 x 5Subtotal 14/20 70%Total Score 25 9 2 36______________________________________________________________________________89

Site 5: Big Walnut Creek at the IR 270 Bridge, at 40 o 01’47.3”N, 82 o 52’40.2”W, 40.006664 o N-82.874567 o W, Franklin Co., Ohio, on 29 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 2 x 32 % state listed species 18% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 85% x 55 % extant individuals 96% x 56 ratio of mean to median age 0.95 x 3Subtotal 13/15 87%Community Structure7 Shannon-Weiner Index 0.9089 x 58 % invasive bivalves 95% x 19 % burying species 64% x 310 total # of extant individuals 43 x 3Subtotal 12/20 60%Total Score 15 15 2 32______________________________________________________________________________90

Site 6: Big Walnut Creek at Airport Golf Course downstream of Pizzuro Park, at 40 o 01’47.3”N,82 o 52’40.2”W, 39.993509 o N -82.857756 o W, Franklin Co., Ohio, on 29 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 1 x 32 % state listed species 14% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 70% x 55 % extant individuals 76% x 56 ratio of mean to median age 0.92 x 3Subtotal 13/15 87%Community Structure7 Shannon-Weiner Index 0.7068 x 38 % invasive bivalves ---% x 19 % burying species 57% x 310 total # of extant individuals 28 x 1Subtotal 8/20 40%Total Score 10 15 3 28______________________________________________________________________________---% - no quadrat data were collected so no invasive species were collected: score assigned = 191

Site 7: Big Walnut Creek at Whitehall Community Center, at 39 o 58’54.5”N, 82 o 51’50.9”W,39.981531 o N -82.863957 o W, Franklin Co., Ohio, on 30 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 0 x 12 % state listed species 0% x 13 # of federal listed species 0 x 1Subtotal 3/15 20%Reproductive Potential4 % extant species 63% x 55 % extant individuals 52% x 36 ratio of mean to median age 0.83 x 5Subtotal 13/15 87%Community Structure7 Shannon-Weiner Index 0.5936 x 38 % invasive bivalves ---% x 19 % burying species 20% x 110 total # of extant individuals 11 x 1Subtotal 6/20 30%Total Score 10 6 6 22______________________________________________________________________________---% - no quadrat data were collected so no invasive species were collected: score assigned = 192

Site 8: Big Walnut Creek at Big Walnut Park, at 39 o 56’57.0”N, 82 o 51’16.3”W, 39.949177 o N-82.854546 o W, Franklin Co., Ohio, on 30 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 4 x 32 % state listed species 27% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 100% x 55 % extant individuals 89% x 56 ratio of mean to median age 0.50 x 5Subtotal 15/15 100%Community Structure7 Shannon-Weiner Index 1.0369 x 58 % invasive bivalves 93% x 19 % burying species 67% x 310 total # of extant individuals 93 x 3Subtotal 12/20 60%Total Score 25 9 2 36______________________________________________________________________________93

Site 9: Big Walnut Creek at Nerfzgel Park, at 39 o 54’47.3”N, 82 o 52’14.0”W, 39.912873 o N-82.870410 o W, Franklin Co., Ohio, on 4 October 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 2 x 32 % state listed species 30% x 53 # of federal listed species 0 x 1Subtotal 9/15 60%Reproductive Potential4 % extant species 50% x 35 % extant individuals 19% x 16 ratio of mean to median age 0.80 x 5Subtotal 9/15 60%Community Structure7 Shannon-Weiner Index 0.7782 x 38 % invasive bivalves ---% x 19 % burying species 67% x 310 total # of extant individuals 6 x 1Subtotal 8/20 40%Total Score 10 12 4 26______________________________________________________________________________---% - no quadrat data were collected so no invasive species were collected: score assigned = 194

Site 10: Big Walnut Creek downstream of Hamilton Road (SR 317), at 39 o 54’36.4”N,82 o 52’57.4”W, 39.910023 o N -82.883249 o W, Franklin Co., Ohio, on 4 October 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 0 x 12 % state listed species 0% x 13 # of federal listed species 0 x 1Subtotal 3/15 20%Reproductive Potential4 % extant species 36% x 35 % extant individuals 26% x 16 ratio of mean to median age 1.09 x 3Subtotal 7/15 47%Community Structure7 Shannon-Weiner Index 0.6021 x 38 % invasive bivalves ---% x 19 % burying species 0% x 110 total # of extant individuals 8 x 1Subtotal 6/20 30%Total Score 0 9 7 16______________________________________________________________________________---% - no quadrat data were collected so no invasive species were collected: score assigned = 195

Site 11: Big Walnut Creek at East Williams Road, at 39 o 53’15.6”N, 82 o 54’18.4”W, 39.887874 o N-82.905006 o W, Franklin Co., Ohio, on 11 October 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 0 x 12 % state listed species 0% x 13 # of federal listed species 0 x 1Subtotal 3/15 20%Reproductive Potential4 % extant species 23% x 15 % extant individuals 30% x 36 ratio of mean to median age 0.89 x 5Subtotal 9/15 60%Community Structure7 Shannon-Weiner Index 0.3768 x 18 % invasive bivalves ---% x 19 % burying species 0% x 110 total # of extant individuals 6 x 1Subtotal 4/20 20%Total Score 5 3 8 16______________________________________________________________________________---% - no quadrat data were collected so no invasive species were collected: score assigned = 196

Site 12: Big Walnut Creek downstream of three rivers MetroPark, at 39 o 52’24.5”N,82 o 54’43.6”W, 39.873349 o N -82.912382 o W, Franklin Co., Ohio, on 11 October 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species2 % state listed species3 # of federal listed speciesSubtotalReproductive Potential4 % extant species5 % extant individuals Analysis not done for this site6 ratio of mean to median ageSubtotalCommunity Structure7 Shannon-Weiner Index8 % invasive bivalves9 % burying species10 total # of extant individualsSubtotalTotal Score______________________________________________________________________________97

Site 13: Big Walnut Creek upstream of London-Groveport Bridge (SR 17), at 39 o 50’00.5”N,82 o 59’30.2”W, 39.833993 o N -82.980494 o W, Franklin Co., Ohio, on 13 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 1 x 32 % state listed species 14% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 78% x 55 % extant individuals 92% x 56 ratio of mean to median age 0.88 x 5Subtotal 15/15 100%Community Structure7 Shannon-Weiner Index 0.5448 x 38 % invasive bivalves 92% x 19 % burying species 43% x 310 total # of extant individuals 57 x 5Subtotal 12/20 60%Total Score 20 12 2 34______________________________________________________________________________98

Site 14: Big Walnut Creek downstream of London-Groveport Bridge (SR 317), at 39 o 49’52.9”N,82 o 59’33.6”W, 39.831382 o N -82.992654 o W, Franklin Co., Ohio, 13 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 1 x 32 % state listed species 17% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 67% x 55 % extant individuals 78% x 56 ratio of mean to median age 0.50 x 5Subtotal 15/15 100%Community Structure7 Shannon-Weiner Index 0.5579 x 38 % invasive bivalves 92% x 19 % burying species 0% x 110 total # of extant individuals 25 x 1Subtotal 6/20 30%Total Score 15 9 4 28______________________________________________________________________________99

Site 15: Big Walnut Creek adjacent to gravel quarry, at 39 o 49’25.3”N, 82 o 59’23.1”W,39.823557 o N -82.990004 o W, Franklin Co., Ohio, on 15 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species2 % state listed species3 # of federal listed speciesSubtotalReproductive Potential4 % extant species5 % extant individuals Analysis not done for this site6 ratio of mean to median ageSubtotalCommunity Structure7 Shannon-Weiner Index8 % invasive bivalves9 % burying species10 total # of extant individualsSubtotalTotal Score______________________________________________________________________________100

Site 16: Big Walnut Creek off of Lockbourne Road, at 39 o 58’11.3”N, 82 o 58’11.3”W,39.819118 o N -82.970161 o W, Franklin Co., Ohio, on 16 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 4 x 32 % state listed species 33% x 53 # of federal listed species 0 x 1Subtotal 9/15 60%Reproductive Potential4 % extant species 52% x 35 % extant individuals 65% x 56 ratio of mean to median age 0.77 x 5Subtotal 13/15 87%Community Structure7 Shannon-Weiner Index 0.4326 x 18 % invasive bivalves 95% x 19 % burying species 33% x 310 total # of extant individuals 53 x 5Subtotal 10/20 50%Total Score 20 9 3 32______________________________________________________________________________101

Site 17: Big Walnut Creek upstream of Rowe Road Bridge (CR 133), at 39 o 48’42.8”N,82 o 58’11.3”W, 39.812071 o N -82.969446 o W, Franklin Co., Ohio, on 16 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 1 x 32 % state listed species 13% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 50% x 35 % extant individuals 76% x 56 ratio of mean to median age 0.95 x 3Subtotal 11/15 73%Community Structure7 Shannon-Weiner Index 0.8537 x 38 % invasive bivalves 93% x 19 % burying species 13% x 110 total # of extant individuals 22 x 1Subtotal 6/20 30%Total Score 5 15 4 24______________________________________________________________________________102

Site 18: Big Walnut Creek downstream of Rowe Road Bridge (CR 133), at 39 o 48’34.6”N,82 o 58’38.8”W, 39.809502 o N -82.977974 o W, Franklin Co., Ohio, on 13 October 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species2 % state listed species3 # of federal listed speciesSubtotalReproductive Potential4 % extant species5 % extant individuals Analysis not done for this site6 ratio of mean to median ageSubtotalCommunity Structure7 Shannon-Weiner Index8 % invasive bivalves9 % burying species10 total # of extant individualsSubtotalTotal Score______________________________________________________________________________103

Site 19: Big Walnut Creek downstream of US Rt. 23 Bridge, at 39 o 47’59.5”N, 83 o 00’14.9”W,39.8806785 o N -82.996485 o W, Franklin Co., Ohio, on 20 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 0 x 12 % state listed species 0% x 13 # of federal listed species 0 x 1Subtotal 3/15 20%Reproductive Potential4 % extant species 38% x 35 % extant individuals 76% x 56 ratio of mean to median age 0.95 x 3Subtotal 11/15 73%Community Structure7 Shannon-Weiner Index 0.5951 x 38 % invasive bivalves ---% x 19 % burying species 0% x 110 total # of extant individuals 29 x 1Subtotal 6/20 30%Total Score 5 9 6 20______________________________________________________________________________---% - no quadrat data were collected so no invasive species were collected: score assigned = 1104

Site 20: Big Walnut Creek further downstream of US Rt. 23 Bridge, at 39 o 47’59.5”N,83 o 00’14.9”W, 39.80012 o N -83.004117 o W, Pickaway Co., Ohio, on 20 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 3 x 32 % state listed species 33% x 53 # of federal listed species 0 x 1Subtotal 9/15 60%Reproductive Potential4 % extant species 64% x 55 % extant individuals 74% x 56 ratio of mean to median age 1.10 x 3Subtotal 13/15 87%Community Structure7 Shannon-Weiner Index 0.7362 x 38 % invasive bivalves ---% x 19 % burying species 33% x 310 total # of extant individuals 34 x 3Subtotal 10/20 50%Total Score 15 15 2 32______________________________________________________________________________---% - no quadrat data were collected so no invasive species were collected: score assigned = 1105

Site 21: Big Walnut Creek just upstream of its mouth, at 39 o 47’57.6”N, 83 o 00’34.4”W,39.799587 o N -83.007799 o W, Pickaway Co., Ohio, on 20 September 2012.Metric Value 5 3 1 Score______________________________________________________________________________Distribution & Abundance1 # of state listed species 1 x 32 % state listed species 25% x 33 # of federal listed species 0 x 1Subtotal 7/15 47%Reproductive Potential4 % extant species 40% x 35 % extant individuals 47% x 36 ratio of mean to median age 1.00 x 3Subtotal 9/15 60%Community Structure7 Shannon-Weiner Index 0.4990 x 38 % invasive bivalves ---% x 19 % burying species 25% x 110 total # of extant individuals 9 x 1Subtotal 6/20 30%Total Score 0 18 4 22______________________________________________________________________________---% - no quadrat data were collected so no invasive species were collected: score assigned = 1106

Appendix 3. Abstract of Ohio Academy of Science presentationMUSSEL COMMUNITY CHANGE IN LOWER BIG WALNUT CREEK. Michael D.Grumney, michael.grumney@otterbein.edu, and Michael A. Hoggarthmhoggarth@otterbein.edu, Otterbein University, Dept. of Biology and Earth Science,Westerville OH 43081.Big Walnut Creek in central Ohio once supported a mussel fauna of 38 species, but nosystematic study of the mussel fauna of the creek has been done. The objective of the currentstudy was to determine the distribution and abundance of mussels in Big Walnut Creekdownstream of Hoover Dam (RM 36.7) to its mouth at the Scioto River. The extant (living andfreshly dead shells) and total (extant plus weathered and subfossil shells) mussel communitieswere determined at 21 sites. Two techniques were used to determine the mussel community ateach site: timed searches and transect/quadrat sampling. Shannon-Weiner (H’) values, JaccardCoefficient of similarity values, and percent extant species were calculated for the musselcommunities at each location. Student T-Tests were used to determine where a significantchange in community structure occurred. The mussel communities from Hoover Dam toWhitehall (RM 22.0) changed very little (Jaccard Coefficient = 83% and percent extant = 78%).H’ values for this reach were not significantly different when comparing the total and extantcommunities (t = 1.08, p > 0.05). However, the communities from RM 22.0 to RM 15.0 (justdownstream of Three Creeks Metropark) had fewer extant species (Jaccard Coefficient andpercent extant values of 62% and 36%) and significantly diminished species diversity (t = 2.35, p< 0.05). Diminished species diversity continued to be expressed downstream (t = 2.48, p < 0.05),with some recovery (Jaccard Coefficient value of 67% and percent extant value of 42%) as aresult of movement of mussels from the Scioto River.Citation:Grumney, Michael D. and Michael A. Hoggarth. 2013. Mussel community change in lower BigWalnut Creek. The Ohio Journal of Science, 113:1): A37.107