Joint Meeting - Genomics - U.S. Department of Energy

Milestone 1
branching structure generated by multiple displacement amplification. A three-step enzymatic treatment
has been developed to resolve the high order DNA structure, so that the chimeric rate has been
reduced from as high as 52% to 6%. We have sequenced two MIT 9312 plones, one at the sequencing
depth of 3.5x and the other at 4.7x, and recovered 62% and 66% of the genome respectively. Full
genome coverage can be achieved by increasing the sequencing depth to ~24x or PCR sequencing
from the plones. The estimated mutation rate in single cell amplification is