Influence of different types of bedding materials ... - Veterinary World

Veterinary World Vol.3(9):418-421RESEARCHInfluence of different types of bedding materialson immune response and serum biochemicalprofile of caged miceVijayakumar . R, R.Samanta*, A.K.Samanta, R. Guria and S. Joardar**Department of Livestock Production Management**Department of Veterinary MicrobiologyFaculty of Veterinary & Animal SciencesWest Bengal University of Animal & Fishery Sciences, Kolkata - 700 037*Corresponding author Email: rajarshisamanta@yahoo.inAbstractContact bedding material is an important environmental factor and welfare creator for laboratory mice. It canalter important physiological process and create potential chance for experimental variation which puts hurdlefor comparability. The present experiment was conducted to assess the possible impact of different types ofbedding material viz CPS, NWS and PH with fifty one albino mice for a period of fifteen weeks in LaboratoryAnimal Facility. It was observed that local immunity in mice was enhanced in NWS. During in vitro immuneassays, mice from NWS showed higher OD value for reactive oxygen radical, produced more NO2 and higherstimulation index i.e. 0.71 ± 0.01, 30.67 ± 0.88 µM and 7.90 ± 0.17, respectively than PH (0.23 ± 0.01, 17.0 ±1.15 µM and 6.33 ± 0.21) and CPS (0.33 ± 0.03, 15.67 ± 1.20 µM and 6.46 ± 0.27). There was no influence ofbedding type on systemic response. Reduced glutathione value in liver was higher in NWS than PH and CPSi.e. 8.54 ± 0.2, 7.09 ± 0.18 and 6.96 ± 0.14 µmole/ gm of tissue. But heart reduced glutathione showed novariation among different types of bedding materials. Serum analysis showed significantly (p

Influence of different types of bedding materials on immune response and serum biochemical profile of micewood shavings (NWS) and (c) paddy husk (PH) were described by (Griffith, 1980) in UV-VIS spectrophotometersteam sterilized at 130° C for 20 minutes and applied atat 412 nm wavelength.a depth of 2 cm in cages and changed at weeklyinterval. For this study NWS bedding was consideredResults and Discussionas enrichment object. The mice were provided ad Immunological parameters: To determine thelibitum acidified water (pH 2.8 to 3.1, monitored external environmental influence on local and systemiccontinuously) and provided with own formulated feed immunity, intestinal mucosal leucocytes as well ascontaining ingredients like crushed wheat, skimmed blood and spleenic leukocytes were assayedmilk powder, cod liver oil, dried yeast, and sodium respectively (Table1). The intestinal mucosachloride (I.V.R.I) and that was autoclaved at 100°C for leukocytes were activated by NWS that lead to more58 min. Light intensity in the room was 200–260 lux, functional and proliferative activity of leukocytes.measured 1 m above the floor. A 12 h light Significant (p

Influence of different types of bedding materials on immune response and serum biochemical profile of miceTable 1: Mean ± S.E of O.D value of Superoxide anion production assay, NaNo 2 (µM) of in vitro nitriteproduction assay and Stimulation Index (S.I) of lymphoproliferation assay for different beddingtypesBedding O.D value NaNo2 (µM) S.IIML Blood IML SL IML SLPH 0.23 ± 0.01b 0.31 ± 0.03b 17.0 ± 1.15b 18.67 ±1.86 6.33 ± 0.21 b 6.88 ± 0.29NWS 0.71 ± 0.01a 0.41 ± 0.01a 30.67 ± 0.88a 22.0 ± 2.31 7.90 ± 0.17 a 6.69 ± 0.32CPS 0.33 ± 0.03b 0.31 ± 0.02ab 15.67 ± 1.20b 19.0 ± 0.58 6.46 ± 0.27 b 6.47 ± 0.26Mean values bearing common superscripts do not differ significantly. (p

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