21.07.2015 Views

绿色植物生长调节剂( GGR)对高寒草甸矮嵩草抗氧化 ... - 中国科学院

绿色植物生长调节剂( GGR)对高寒草甸矮嵩草抗氧化 ... - 中国科学院

绿色植物生长调节剂( GGR)对高寒草甸矮嵩草抗氧化 ... - 中国科学院

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

18 1 Vol. 18 No. 1 ACTA A GRESTIA SINICA2010 1 Jan. 2010( <strong>GGR</strong>) 1 , 2 ,3 , 1 3 , 1 , 1 , 1(1. , 810008 ; 2. , 610041 ;3. , 100039): ( Kobresia humilis (C. A. Mey ex Trauvt) Serg. ) ,( <strong>GGR</strong>) :(SOD) 6 8 ( P < 0. 05) ,7 ;( GSH) ;S ,V,( P


1 :( <strong>GGR</strong>) 57,,( <strong>GGR</strong>) , <strong>GGR</strong> [4 ] [5 ] ,, <strong>GGR</strong> ,, [6 ] <strong>GGR</strong> ,,<strong>GGR</strong> ,, 1 ,, 420 - 560 mm ,5 - 10 1. 2 [7 ] ,( Ko2AB T ,) ,375 kg hm - 2 (C K) 6 - 9 ,,40 ,,,1. 3 :5 mL 0. 15 mol L - 1(p H 7. 0 ,0. 3 % PV P) ,,15000 r min - 1 10 min ,( SOD) Giannopiolitis Ries [8 ] ;( GSH) Ellman [9 ] ;Arnon [10 ] [11 ] ;G2250 1. 1 [12 ] ; [13 ] ;, [14 ]4 ,,5 ,6 ,,9 1. 4 SPSS 13. 0 ,(33 43 - 35 16 N , 98 48 - 100 55 E ,4000 mSigmaPlot 10. 0 ) ,,,7 - 8 ,;,4 - 5 0 2 ,,, 2. 1 <strong>GGR</strong>SOD GSH ,SOD 7 (1A) ,U , (6 ) (9 ),(7 8 ) ,, 6 8 SOD ( P


58 18 1 <strong>GGR</strong>SOD GSHFig. 1 Effect of <strong>GGR</strong> on the activity of SOD and contents of GSH in the leaves of K. humilis<strong>GGR</strong> GSH 2. 2 <strong>GGR</strong>222S,V(2A) ,9 ( P < 0. 05) ,9 <strong>GGR</strong> ,8 ,( P < 0. 05) (2B) ,V,V 6 ,7 8 ( P


1 :( <strong>GGR</strong>) 593 <strong>GGR</strong>Fig. 3 Effect of <strong>GGR</strong> on the contents of chlorophyll in the leaves of K.humilis4 <strong>GGR</strong>O -2 Fig. 4 Effect of <strong>GGR</strong> on the generate velocity of3 O - 2 in the leaves of K. humilisDNA [15 ] ,(SOD GSH AsA ) ROS ,ROS SOD ,,O - 2H2 O2 , [16 ] , [17 ] GSH ,, GSH2AsA ROS [18 ], GSH [ 19 ] GSH ,SOD ,, [5 ] ,SOD ,, , [20 ],<strong>GGR</strong> SOD <strong>GGR</strong> [5 21 22 ] ,,,, ,, [7 ] , [23 ] ( Caragana Fabr. ) , ,,( ROS) , ,ROS 9 ,,,ROS ,, [24 ] ,7 8 , , [24 ] ; ncel [25 ] , [22 ] <strong>GGR</strong> ,,RNA © 1994-2010 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net


60 18 [26 ] ,, , , [27 ] <strong>GGR</strong> ,,,, [6 ] ,<strong>GGR</strong> [ 28 ] , <strong>GGR</strong> ,,,O -2 ,H2 O2 ,,, [29 ] <strong>GGR</strong> O -2 ,<strong>GGR</strong> O - 2,,,<strong>GGR</strong> 4 <strong>GGR</strong> SOD ,GSH <strong>GGR</strong> :;<strong>GGR</strong> ,,O - 2,[1 ] . [J ] .,2003 ,12 (6) :12 9[ 2 ] , ,,. [J ]. ,2008 ,27 (5) : 6972704[ 3 ] ,,,. [J ] . ,2009 ,17 (5) :6292635[ 4 ] ,,. [J ] . ,2002 ,8 (5) :39240[5 ] . [ D ]() . :,2006 ,324[ 6 ] ,,,. ( <strong>GGR</strong>) [J ] . ,2008 ,20 (3) :15217[ 7 ] ,,,. [J ]. ,2005 ,14 (3) :31240[ 8 ] Giannopolitis C N , Ries S K. Superoxide dismutase II. Purifi2cation and quantitative relationship wit h water soluble proteinin seedlings [J ] . Plant Physio1ogy , 1997 , 59 : 3152318[ 9 ] Ellman G L . Tissue sulf hydryl groups [J ] . Archives of Bio2chemistry and Biophysics , 1959 , 82 : 70277[ 10 ] Arnon D I. Copper enzymes in isolated chloroplast s : polyphe2noloxidase in Beta vul garis [J ]. Plant Physiology , 1949 , 24 :1215[ 11 ] . [ M ] . :,1990 ,46[ 12 ] Braford M M. A rapid and sensitive met hod for t he quantiza2tion of microgram quantities of protein utilizing t he principle ofprotein2dye binding [J ] . Analytical Biochemistry , 1976 , 72 :2482254[ 13 ] ,,. [ M ] . :,2004 ,65267[ 14 ] . [ M ] . :,2004. 3082309[ 15 ] Franga M B , Panek A D , Eleut herio E C A. Oxidative stressand it s effect s during dehydration [J ] . Comparative Biochemis2try and Physiology2Part A : Molecular & Integrative Physiolo2gy , 2007 , 146 : 6212631[ 16 ] ,,. [J ] . ,2005 ,29 (2) :3312337[ 17 ] Foyer CH , Halliwell B. The presence of glutat hione and glu2tat hione reductase in chloroplast : a proposed role in ascorbicacid metabolism [J ] . Planta , 1976 , 133 : 21225[ 18 ] Noctor G , Foyer C H. Ascorbate and glutat hione : keeping ac2tive oxygen under cont rol [J ] . Annual Review of Plant Physi2ology and Plant Molecular Biology , 1998 , 49 : 2492279[ 19 ] Asada K. The water2water cycle in chloroplast s : scavenging ofactive oxygen and dissipation of excess photons [J ] . AnnualReview of Plant Physiology and Plant Molecular Biology ,1999 , 50 : 6012639[ 20 ] ,,,. [J ] . ,2009 ,17 (4) :4702473[ 21 ] . ( <strong>GGR</strong>) [J ] . ,2006 ,5 :9210[ 22 ] ,. AB T <strong>GGR</strong> NAA [J ] . ,2004 ,2 :11212[ 23 ] ,,,. 5 [J ] . () ,2005 ,36 (6) :6772682[ 24 ] ,,. [J ] . ,1997 ,27 (2) :972104[ 25 ] ; ncel I , Yurdakulol E , Kele, Y , et al . Role of antioxidant de2fense system and biochemical adaptation on st ress tolerance ofhigh mountain and steppe plant s [J ] . Acta Oecologica , 2004 ,26 : 2112218[26 ] ,,. [J ] . ,2003 ,20 (5) :5392548[ 27 ] ,,,. [J ] . ,2003 ,9 (5) :4892492[ 28 ] Ren H X , Wang Z L , Chen X , et al . Antioxidative responsesto different altitudes in Plantago maj or [J ] . Environmentaland Experimental Botany , 1999 , 42 : 51259[ 29 ] ,. [J ] . ,2000 ,17 (6) :5592564( )© 1994-2010 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!