2011-2012 Catalogue - PeproTech, Inc.

PEPROTECH ®www.peprotech.comManufacturer of Quality Cytokine Products®Copyright © 2011 by PeproTech Inc.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se1

www.peprotech.com2011-2012 Catalogue2To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comAbout PeproTechPeproTech was established in 1988 by a group of scientists who decided to focus their efforts on the development andproduction of recombinant cytokines for life-science research. Today, PeproTech is a world leader in supplying high qualitycytokine products including E. coli, insect, and mammalian cell-derived recombinant proteins, their monoclonal/polyclonalantibodies, ELISA Development Kits, and other cytokine-related reagents. These products are distributed worldwidethrough customer-service offices in the US, UK, France, Germany, Israel, Czech Republic, Korea, China, Japan, Brazil,and Mexico. We plan to maintain the highly competitive price of our products and to expand their distribution to Australia,New Zealand, and other locations. To keep up with current research and growing demand we have recently expanded ourproduction capabilities to include a 5000 sq. ft. animal-free manufacturing facility. A key component in keeping up withcurrent scientific advancements has been our collaboration with researchers in both academia and industry who provide uswith suggestions necessary to continuously expand our product pipeline. We encourage you to convey to us your particularresearch needs and promise to do our best to fulfill them.A propos de PeproTechPeproTech a été crée en 1988 par un groupe de scientifiques qui ont concentré leurs efforts sur le développement et laproduction de cytokines recombinantes pour la recherche en science de la vie. Aujourd’hui, PeproTech est un fournisseurmondial de réactifs de haute qualité composés de Protéines recombinantes dérivées de E. Coli, de cellules d’insectes& mammifères. PeproTech fabrique également des Anticorps polyclonaux et monoclonaux, des kits ELISA et autresréactifs en relation avec les cytokines. Ces produits sont distribués à travers le monde grâce à nos bureaux basés auxEtats-Unis, au Royaume – Uni, en France, en Allemagne, en Israël, en République Tchèque, en Corée, en Chine, auJapon, au Brésil et au Mexique. Nous souhaitons maintenir les prix très compétitifs de nos produits et étendre notredistribution á l´Australie, Nouvelle Zélande et autres territoires. Afin de poursuivre nos recherches et de répondre à lademande croissante, nous avons agrandi nos laboratoires par l’acquisition d’une surface de production de 5000m2 pouraccueillir le site de production « animal-free ». Le facteur clé de la progression de nos recherches scientifiques a été notrecollaboration avec les laboratoires publics & privés qui nous ont fait part de leurs suggestions permettant de développernos gammes de produits. Nous vous encourageons à nous faire part de vos besoins particuliers pour vos recherches etnous vous promettons de faire au mieux pour y répondre.Über PeproTechPeproTech wurde 1988 von einer Gruppe Wissenschaftler gegründet, die entschieden hatten, sich auf Entwicklung undProduktion rekombinanter Zytokine für die Life-Science Forschung zu konzentrieren.Heute ist PeproTech ein weltweit führender Hersteller qualitativ hochwertiger Zytokine sowie verwandter Produkte.Die Produktpalette beinhaltet aus E.coli, Insektenzellen und Säugerzellen gewonnene rekombinante Proteine, dieentsprechenden Antikorper, ELISA-Development Kits, und andere, Zytokin-verwandte Reagenzien. Wir vertreibenunsere Produkte weltweit über Kundenservice Büros in den USA, England, Frankreich, Deutschland, Israel, derTschechischen Republik, Korea, China, Japan, Brasilien und Mexiko. Wir planen die wettbewerbsfähigen Preise und diehervorragende Qualität unserer Produkte beizubehalten und den Vertrieb auf andere Länder, wie zum Beispiel Australienund Neuseeland, auszuweiten. Um mit der aktuellen Forschung und der steigenden Nachfrage mitzuhalten, haben wirunsere Produktion mit einer 550 qm grossen Anlage erweitert, in der ausschliesslich Produkte hergestellt werden, diefrei von tierischen Bestandteilen (ADCF) sind. Unsere sehr enge Zusammenarbeit mit Forschern aus Universitäten undder Industrie, ermöglicht es uns unser Produktportfolio ständig zu erweitern, und so mit dem Fortschritt der aktuellenWissenschaft Schritt zu halten. Wir möchten daher auch Sie bitten, uns Ihre Bedürfnisse mitzuteilen, und wir werdenunser Bestes geben um diese zu realisieren.O PeproTechuSpolečnost PeproTech byla založena v roce 1988 skupinou odborníků, kteří se rozhodli zaměřit svou vědeckou práci navývoj a výrobu rekombinantních cytokinů pro využití v biomedicínském výzkumu. Peprotech je v současnosti světovoujedničkou mezi dodavateli produktů v oblasti cytokinů dosahujících vysoké kvality. Vedle cytokinů zahrnuje naše produkcetaké další rekombinantní proteiny derivované z E.coli, buněk insektu a savců, dále jejich monoklonální/polyklonální protilátky,ELISA Development Kity a další, s cytokiny související, látky. Všechny tyto produkty jsou celosvětově distribuoványnašimi kancelářemi v USA, Kanadě, UK, Francii, Německu, Izraeli, Koreji, Číně, Brazílii a Mexiku. Naším cílem je udrženíbezkonkurenčních cen našich produktů a rozšíření distribuce v Austrálii, na Novém Zélandě a v dalších zemích. Abychomudrželi krok se současným výzkumem a narůstajícími nároky, tak jsme nedávno rozšířili naši výrobní kapacitu, včetně470 m 2 určených pro výrobu našich „Animal-free“ produktů. Klíčovou součástí našeho rozvoje je spolupráce s vědeckýmipracovníky, jak z akademické sféry, tak i z průmyslu. To oni nám poskytují podněty důležité k neustálému rozšiřování našíproduktové řady. Budeme rádi, když nám sdělíte své konkrétní potřeby pro váš výzkum a slibujeme, že uděláme vše, coje v našich silách, abychom je uspokojili.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se3

www.peprotech.com2011-2012 CatalogueTable of ContentsLook for PeproTech ............................................................................................................... (below)About PeproTech............................................................................................................................3Ordering Information......................................................................................................................5PeproTech Worldwide Directory.....................................................................................................6Tradeshows 2011............................................................................................................................7PeproTech Publications..................................................................................................................8ELISA Buffer Kits............................................................................................................................9Animal-Free Media Supplements.................................................................................................10Animal-Free Recombinant Proteins..............................................................................................11Products (Listed Alphabetically)............................................................................................12-141Control Antibodies......................................................................................................................141Charts & Tables:Chemokine Nomenclature.................................................................................................142FGF Family Table........................................................................................................143-144TNF Superfamily Nomenclature........................................................................................145TGF-β Superfamily Table............................................................................................146-148Antagonists of TGF-β Ligand..............................................................................................149General Characteristics of Plasma Lipoproteins................................................................150Classification of Apoproteins.............................................................................................150General Abbreviations ...............................................................................................................151Technical Guide:Cytokine FAQ.....................................................................................................................152Animal-Free Media Supplement Kits FAQ.........................................................................153Antigen-Affinity Purification Protocol.........................................................................154-156General Antibody FAQ.......................................................................................................157ELISA Protocol............................................................................................................158-160ELISA FAQ.........................................................................................................................161ELISA Troubleshooting Guide.....................................................................................162-163Western Transfer Protocol...........................................................................................164-165Western Transfer FAQ........................................................................................................166Western Transfer Troubleshooting Guide....................................................................167-171Immunohistochemistry General Protocol...................................................................172-173Immunohistochemistry Troubleshooting Guide..........................................................174-176Microarray Protocol....................................................................................................177-179Index ...................................................................................................................................180-187Look for PeproTech…PeproTech representatives routinely attend scientific conferences throughout the year, e.g.,International Society for Stem Cell Research, Amercian Society of Cell Biology, American Associationfor Cancer Research, Experimental Biology, and others. We welcome you to visit our booth tocommunicate your research needs, and to discuss our latest products. Please visit our website atwww.peprotech.com for our conference schedule.4To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comOrdering InformationOur normal business hours are from 9:00 AM to 5:00 PM Monday - Friday. To place an order or requesta Catalogue after business hours, or on weekends, you may send us a fax or visit our website.To Place an Order:European Headquarters (for countries not listed below):Tel: +44 (0)20 7610 3062, or +44 (0)20 7610 3055 • Fax +44 (0)20 7610 3430info@peprotech.co.ukAUSTRIA:Tel: +43 (0)1 405 9696 • Fax +43 (0)1 405 9898 • info@peprotech.atCZECH REPUBLIC:Tel: +420 225 992 284 • Fax +420 225 992 201 • info@peprotech.czFRANCE:Tel: +33 (0)1 46 24 58 20 • Fax: +33 (0)1 46 24 01 66 • info@peprotechfr.comGERMANY:Tel: 0800 436 99 10, or +49 (0)40 734 35 77 70 • Fax +49 (0)40 734 35 77 79 • info@peprotech.deNORDIC:Tel: +46 (0)8 640 41 07 • Fax +46 (0)8 640 41 09 • info@peprotech.seWEBSITE: www.peprotech.comMethod of Payment:Payment terms are net thirty (30) days.1.5% interest per month may be charged on past due accounts.VISA and MasterCardaccepted.Shipping:PeproTech's products have been formulated to allow shipment at ambient temperature. If your purchase order isreceived before 4:00 pm, all available items are shipped by courier service for next day delivery.Return Policy:Any claim for credit or return goods must be made within 7 days of receipt. PeproTech will not accept returnedproducts without prior authorization. No credit or replacement will be issued without a return authorization number.All returns are subject to a 25% return fee unless the products are returned due to lack of conformity with thespecifications in PeproTech's data sheets. Please call Customer Service for return instructions.Warranty:PeproTech warrants to the original purchaser that products sold substantially conform to specifications provided withshipped products and are free from material defects. This warranty does not extend to any product which has beenaltered in anyway by personnel other than PeproTech's employees or to any product which has been handled in a mannercontrary to instructions included in product documentation. PeproTech is not responsible for patent infringementor other violations which may occur with the use of these products. PeproTech makes no warranty of the product'ssuitability for any purchaser's particular use; suitability for use must be determined by the purchaser or user of theproducts. All other warranties, expressed or implied are disclaimed to the fullest extent allowed by law. Purchaser'ssole remedy under the above warranty shall be replacement with a conforming product, a credit toward future purchasesof PeproTech products or, at PeproTech's sole discretion, a refund of the price of the product. If there is aquestion or concern please contact our Technical Support Department immediately so we may assist you.Resale:In the absence of an expressed written agreement to the contrary, all products are sold by PeproTech Inc. for theexclusive use of the purchaser and are not to be resold.Information Changes:This Catalogueue contains current information on PeproTech products. PeproTech reserves the right to discontinuesales of any product or to change product descriptions and/or formulations at any time without notice to purchasers.Product Use Limitations:PeproTech products are offered for research use only. Not for drug use or clinical diagnostic procedures.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se5

www.peprotech.com2011-2012 CataloguePeproTech - WorldwideUSA & JapanPrinceton Business Park5 Crescent Ave.P.O. Box 275Rocky Hill, NJ 08553-0275U.S.A.Tel: (800) 436-9910(609) 497-0253Fax: (609) 497-0321email:info@peprotech.comsales@peprotech.comwww.peprotech.comCanada4280, boul. Saint JeanSuite 203Dollard des OrmeauxQuebecCanada H9H 2A5Tel: (877) 696-1982Fax: (877) 696-6998email:info@peprotechcanada.comwww.peprotech.comLatin & South AmericaLuz Saviñon No. 1027-4Col. NarvarteDel. Benito JuarezMéxico, DFTel: (52) (55) 5672-0389Fax: (52) (55) 5672-0388email:pepromex@prodigy.net.mxwww.peprotech.comEuropePeproTech House29 Margravine RoadLondon W6 8LLEnglandTel: +44 (0)20 7610 3062Fax: +44 (0)20 7610 3430email: info@peprotech.co.ukwww.peprotech.comAustriaSchwendergasse 171150 WienAustriaTel: +43 (0)1 405 9696Fax: +43 (0)1 405 9898email: info@peprotech.atwww.peprotech.comCzech RepublicGemini - Budova BNa Pankráci 129/1724140 00 - Praha 4Czech RepublicTel: +420 225 992 284Fax: +420 225 992 201email: info@peprotech.czwww.peprotech.comFrance12 rue Paul Chatrousse92200 Neuilly-Sur-SeineFranceTel: +33 (0)1 46 24 58 20Fax: +33 (0)1 46 24 01 66email: info@peprotechfr.comwww.peprotech.comAsia, Africa & Australia12 Hamada StreetTamar BuildingRehovot, 76703IsraelTel: +972 8 946 0948Fax: +972 8 946 0861email:info@peprotechasia.comwww.peprotech.comChinaRoom 1918 Gold River Int.BuildingNo. 35, Shishan RoadSuzhou, Jiangsu ProvinceP. R. China, 215011Tel: +86 512 6832 5993Fax: +86 512 6832 5983email: china@peprotech.comwww.peprotech.comKorea1017, 3rd ComplexKing’s Garden B/DNaesu-Dong, Jongro-Gu,Seoul 110-872, KoreaTel: 82-2-3210-2808Fax: 82-2-3210-2835e-mail: info@peprotech.co.krwww.peprotech.comGermanyOberaltenallee 8D-22081 HamburgGermanyTel: +49 (0)40 734 35 77 70(0)800 436 99 10Fax: +49 (0)40 734 35 77 79email: info@peprotech.dewww.peprotech.comNordicKlarabergsviadukten 70107 24 StockholmSwedenTel: +46 (0)8 640 41 07Fax: +46 (0)8 640 41 09email: info@peprotech.sewww.peprotech.com6To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comPeproTech Trade Shows 2011Date Name of Show LocationMarch 1-2 The 10th Congress of the Japanese Society for Regenerative Medicine Tokyo, JapanMarch 30 - April 1 UK National Stem Cell Network Meeting York, EnglandApril 2-6 American Association for Cancer Research (AACR) Orlando, FLApril 6-8 Stem Cell Technology Application Forum 2011 Hangzhou,Zhejiang Province, ChinaApril 9-13 Experimental Biology Washington, DCApril 18-19 British Microcirculation Meeting London, EnglandApril 27-30 Joint Spring Meeting of the British Society of Cell Biology Canterbury, England& British Society of Developmental Biology (BSCB/BSDB)May 8-11 IL-1 Family of Cytokines Conference Clearwater, FLMay 13-17 Immunology 2011 San Francisco, CAMay 18-21 17th Annual Meeting of the International Society Rotterdam,for Cellular Therapy (ISCT)The NetherlandsMay 18-19 NIH Spring Research Festival Bethesda, MDMay 23-24 Israeli Stem Cell Society 4th annual meeting Beit Sourasky, IsraelMay 25-27 13th Annual Meeting of the French Society of Biology Paris, Franceof Mineralised Tissues (SFBTM)May 25-27 Cancer Immunotherapy Meeting (CIMT) Mainz, GermanyMay 26-29 17th Symposium of Czech Reproductive Immunology Zdar nad Sazavou,Czech RepublicMay 29 - June 1 Canadian Neuroscience Meeting Quebec City, CanadaMay 30 - June 1 Colloque des Cytokines Le Croisic, FranceJune 3-4 The 2011 Conference of The Korean Association of Immunologists Seoul, South KoreaJune 9-12 16th Congress of the European Hematology Association London, EnglandJune 15-18 International Society for Stem Cell Research (ISSCR) Toronto, ON CanadaJune 21-25 9th International Cerebral Vascular Biology Conference Leiden, The NetherlandsJune 25-29 10th World Congress on Inflammation Paris, FranceJuly 15-18 12th Congress of Cell Biology (CSCB) BeijingJuly 29- August 1 9th Annual Congress of Neuroscience (CNS) Zhengzhou,Henan Province, ChinaAugust* Universidad Nacional Autonoma de Mexico Mexico City, MexicoAugust* The 9th Catholic International Stem Cell Symposium Seoul, South KoreaSeptember* Universidad Autonoma Metropolitana Mexico City, MexicoSeptember 11-14 Stem Cells in Development and Disease Berlin, GermanySeptember 22-24 Annual European Macrophage & Dendritic Cell Society Meeting (EMDS) Brussels, BelgiumSeptember 22-24 Society for Leukocyte Biology Kansas City, MOSeptember 26 -28 41st Annual Meeting of the German & Italian Society for Immunology Riccione, ItalyOctober* 2011 Annual Meeting of Korean Society of Stem Cell Research Seoul, South KoreaOctober 9-12 ISICR & ICS Annual Meeting Florence, ItalyOctober 11-13 Biotechnica Hannover, GermanyNovember* Latin America Congress of Immunology Lima, PeruNovember 6-9 National Cancer Research Institute Meeting (NICR) Liverpool, EnglandNovember 13-16 Society for Neuroscience Washington, DCNovember 16-19 Medica Dusseldorf, GermanyDecember 3-7 The American Society for Cell Biology Denver, CODecember 4-8 World Allergy Congress XXII Cancun, MexicoDecember 5-9 Annual Meeting of the British Society of Immunology (BSI) Liverpool, EnglandThe Tradeshow Schedule above is tentative and is subject to change.Please visit our website, www.peprotech.com for the most up-to-date schedule.* Dates undetermined at time of printing.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se7

www.peprotech.com2011-2012 CataloguePeproTech PublicationsPeproTech’s publications are intended to provide updated information about our products, and to reviewcurrent trends in life science research. To request any of these complimentary publications please contactus at your local PeproTech office. Contact information on page 6.The UPDATE, is a newsletterpublished on an annual basis.NEW!2011-2012 USBCatalogueOur new USB driveCatalogue containsall the currentPeproTech products,and technicalsupport information.The FOCUS is a collection ofarticles that have been publishedin previous UPDATE editions.2011 Weekly PlannerPeproTech’s 52 weekplanner/calendar.Posters:ChemokinesThis 17” x 24” color poster displays theSystematic Nomenclature for Chemokines.FGF FamilyThis 17” x 24” color poster displays the FGFcytokine family and includes target cells,receptors, and more.TGFb SuperfamilyThis 20” x 26” color poster includesrelevant information about the TGFβSuperfamily.Cytokine INDEX (3rd ed.)This 312 page INDEX containsencyclopedic coverage of morethan 700 cytokines.TNF SuperfamilyThis 17” x 24” color posterdisplays the TNF Superfamilynomenclature.VEGF/PDGF FamilyThis 17” x 24” color poster includesrelavant information about VEGF/PDGF members.Price ListThis 44 page booklet contains thelatest products and purchasinginformation.8To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comPeproTech’s NewELISA Buffer KitPeproTech is pleased to announce therelease of its ELISA Buffer Kit. The buffersin this supplemental kit have been speciallyformulated for optimal performance whenused in conjunction with PeproTech’s ELISADevelopment Kits. The buffer kit containsall of the necessary components to assayten 96 well ELISA plates (included) andcontains detailed handling instructions. Allof the reagents have been sterile filtered tominimize assay interference and maximizeshelf life. The ELISA Buffer Kit can bepurchased along with PeproTech’s ELISADevelopment Kit at a combined packageprice.This easy-to-use ELISA buffer kit can alsobe purchased as a stand-alone productsince the plates included are not pre-coatedwith capture antibody. This format allowsthe researcher to develop and optimize anassay for use with their own capture anddetection antibodies. The actual antibodyconcentrations and detection ranges of theELISA will vary.Kit Contents:- 20X Plate Coating Buffer- 1X Blocking Buffer- 20X Sample Diluent- 20X Wash Buffer- Ready-to-use ABTS- 10 Sterile, Uncoated 96 well ELISA Plates- 50 Plate Sealing Films- Buffer Handling InstructionsELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se9

PEPROTECH ®www.peprotech.comAnimal-Free Recombinant ProteinsTo keep up with current research and growing demand we have recently expanded ourproduction capabilities to include a 5000 sq. ft., animal-free manufacturing facility. Ourgoal is to provide you with animal-free recombinant proteins and other reagents capableof equaling or enhancing the performance of animal-derived media formulations. Pleasecontact your local office for a list of animal-free products currently available as well asthose in our pipeline.Currently Available Animal-Free Recombinant ProteinsProduct Name Catalogue Number Size BHuman BDNF..................................................... AF-450-02........................................................ 10 mgHuman BMP-4 .................................................AF-120-05ET..................................................... 10 mgHuman EGF ...................................................... AF-100-15...................................................... 500 mgHuman FGF-acidic............................................AF-100-17A...................................................... 50 mgHuman FGF-basic ............................................AF-100-18B...................................................... 50 mgHuman flt3-Ligand............................................. AF-300-19........................................................ 10 mgHuman GM-CSF................................................ AF-300-03........................................................ 20 mgHuman Heregulinb-1 ......................................... AF-100-03........................................................ 50 mgHuman IFN-g...................................................... AF-300-02...................................................... 100 mgHuman IGF-I ..................................................... AF-100-11...................................................... 100 mgHuman IL-1b ...................................................AF-200-01B...................................................... 10 mgHuman IL-2 ...................................................... AF-200-02........................................................ 50 mgHuman IL-3........................................................ AF-200-03........................................................ 10 mgHuman IL-4 ....................................................... AF-200-04........................................................ 20 mgHuman IL-6 ....................................................... AF-200-06........................................................ 20 mgHuman IL-15 ..................................................... AF-200-15........................................................ 10 mgHuman IL-16 ....................................................AF-200-16A...................................................... 10 mgHuman IL-17 ..................................................... AF-200-17........................................................ 25 mgHuman IL-21 ..................................................... AF-200-21........................................................ 10 mgHuman KGF....................................................... AF-100-19........................................................ 10 mgHuman M-CSF .................................................. AF-300-25........................................................ 10 mgHuman MCP-1 ................................................... AF-300-04........................................................ 20 mgHuman b-NGF.................................................... AF-450-01........................................................ 20 mgHuman PDGF-BB ............................................AF-100-14B...................................................... 10 mgHuman PlGF-1 ................................................... AF-100-06........................................................ 25 mgHuman SCF ....................................................... AF-300-07........................................................ 10 mgHuman TNF-a ..................................................AF-300-01A...................................................... 50 mgHuman TPO........................................................ AF-300-18........................................................ 10 mgHuman VEGF..................................................... AF-100-20........................................................ 10 mgMurine IL-3........................................................ AF-213-13........................................................ 10 mgMurine IL-4 ....................................................... AF-214-14........................................................ 20 mgMurine GM-CSF................................................ AF-315-03........................................................ 20 mgMurine M-CSF .................................................. AF-315-02........................................................ 10 mgMurine SCF ....................................................... AF-250-03........................................................ 10 mgRat TPO.............................................................. AF-400-34........................................................ 10 mgBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se11

www.peprotech.com2011-2012 CatalogueActivin ASynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Activin A 120-14 Insect cells 26.0 kDa 1µg 5µg 1mgHuman Activin A 120-14E E.coli 26.0 kDa 2µg 10µg 1mgAdditional sizes available please inquireBioassay analysis of human Activin A.Determined by its ability to inhibit theproliferation of mouse MCP-11 cells.OD (490 nm)2.001.601.200.800.40Bioassay for Human Activin A using MPC-11 cells0.000.00 0.01 0.10 1.00 10.00h-Activin A (ng/ml) [log scale]AHuman Activin A; A; PeproTech; Cat# 120-14EActivin BHUMAN Human ACTIVIN Activin A; A; PEPROTECH; PeproTech; CAT# Cat# 120-14Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Activin B 120-15 Insect cells 25.6 kDa 1µg 5µg 1mgAdditional sizes available please inquireAdiponectinSynonyms: Acrp30, apM-1, adipoQ, gelatin-binding protein, 28kD (GBP28)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Adiponectin 450-24 Insect cells 25.0-36.0 kDa 5µg 25µg 1mgAdditional sizes available please inquireAITRL Activation-induced TNFR member ligandSynonyms: TNFSF18, GITRL, TL6Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman AITRL 310-22 E.coli 14.3 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human AITRL 500-P244 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human AITRL 500-P244bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested12To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comAminopeptidaseSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BAeromonas Aminopeptidase 100-10 E.coli 31.4 kDa 100µg 500µg 1mgAdditional sizes available please inquireAmphiregulinSynonyms: ARRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Amphiregulin 100-55B E.coli 11.3 kDa 10μg 50μg 1mgAdditional sizes available please inquireAANG-1 Angiopoietin-1Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Angiopoietin-1 130-06 HeLa cells 60.0-70.0 kDa 5µg 20µg 1mgAdditional sizes available please inquireApoA-1 Apolipoprotein A-1Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman ApoA-1 350-11 E.coli 28.2 kDa 20µg 100µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se13

www.peprotech.com2011-2012 CatalogueApoE2 Apolipoprotein E2Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman ApoE2 350-12 E.coli 34.3 kDa 100µg 500µg 1mgAdditional sizes available please inquireApoE3 Apolipoprotein E3Synonyms: noneARecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman ApoE3 350-02 E.coli 34.0 kDa 100µg 500µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human ApoE3 500-P238 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human ApoE3 500-P238bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedApoE4 Apolipoprotein E4Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman ApoE4 350-04 E.coli 34.4 kDa 100µg 500µg 1mgAdditional sizes available please inquireApo-SAASynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Apo-SAA 300-13 E.coli 11.7 kDa 10µg 50µg 1mgHuman Apo-SAA1 300-53 E.coli 11.7 kDa 10µg 50µg 1mgAdditional sizes available please inquire14To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comAPRIL A proliferating-inducing ligandSynonyms: TNFSF13, TRDL-1αRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman APRIL 310-10C Insect cells 16.3 kDa 2µg 10µg N/AMurine APRIL 315-13 E.coli 21.9 kDa 5µg 20µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human APRIL 500-P192 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human APRIL 500-P192bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedHuman carcinoid (FFPE) stained with rabbit antihumanAPRIL at 0.25 µg/mL. Staining of tumor cells.Tissue samples were provided by the Cooperative Human Tissue Network,which is funded by the National Cancer Institute. Other investigators mayhave received specimens from the same subjects. Photograph provided byPatsy Ruegg for PeproTech.AArteminSynonyms: ARTRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Artemin 450-17 E.coli 24.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Artemin 500-P245 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human Artemin 500-P245bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedkDa148Representative Western Transfer (Blot) analysis ofhuman Artemin using Rabbit anti-human Arteminat 0.20µg/ml.604230221764Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se15

www.peprotech.com2011-2012 Catalogue4-1BBLSynonyms: TNFSF9, CD137LRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman 4-1BBL 310-11 E.coli 19.5 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human 4-1BBL 500-P169 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human 4-1BBL 500-P169bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested4-1BB (Receptor)Synonyms: TNFRSF9, CD137 antigenBRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman 4-1BB 310-15 E.coli 17.7 kDa 5µg 20µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human 4-1BB receptor 500-P167G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human 4-1BB receptor 500-P167Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human 4-1BB receptor Catalogue # 900-K208ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)B7-1Polyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-murine B7-1 500-P128 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine B7-1 500-P128bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested16To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comBAFF B-cell activating factor belonging to the TNF familySynonyms: TNFSF13B, TALL-1, BLyS, THANK, zTNF4Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BAFF 310-13 E.coli 17.0 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human BAFF 500-P163G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human BAFF 500-P163Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human BAFF 500-M64 Mouse E 500µgBAFF enhances Ig secretion by human B cellsco-stimulated through the BcR. Ig secretion wasdetermined using heavy chain specific ELISAsafter 12-14 days of culture. (S.G. Tangye et. al.Seminars in Immunology 18 (2006) 305-317.BBAFF Receptor B-cell activating factor belonging to the TNF receptor familySynonyms: BAFFR, TNFRSF13CRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BAFF Receptor 310-13R E.coli 7.7 kDa 10µg 50µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se17

www.peprotech.com2011-2012 CatalogueBCA-1/BLC (CXCL13) B cell attracting chemokine-1Synonyms: CXCL13, BLC (murine), BLR1 LigandRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BCA-1 300-47 E.coli 10.1 kDa 5µg 20µg 1mgMurine BCA-1/BLC 250-24 E.coli 9.8 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human BCA-1 500-P141 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human BCA-1 500-P141bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBChemotaxis analysis of human BCA-1. Data showsthe migration of human peripheral blood lymphocytesin response to human BCA-1.BCMA B cell maturation antigenSynonyms: TNFRSF17, BCMRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BCMA 310-16 E.coli 5.3 kDa 5µg 20µg 1mgAdditional sizes available please inquire18To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comBD-1,-2,-3,-4 β-Defensins 1-4Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BD-1 (36 a.a.) 300-51 E.coli 3.9 kDa 5µg 20µg 1mgHuman BD-1 (47 a.a.) 300-51A E.coli 5.0 kDa 5µg 20µg 1mgHuman BD-2 300-49 E.coli 4.3 kDa 5µg 20µg 1mgHuman BD-3 300-52 E.coli 5.1 kDa 5µg 20µg 1mgHuman BD-4 300-65 E.coli 5.9 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human BD-1 500-P253 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human BD-1 500-P253bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human BD-2 500-P161G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human BD-2 500-P161Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-human BD-3 500-P241 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human BD-3 500-P241bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human BD-4 500-P268 Rabbit E, WB, NA 50μg 100μg 1mgBiot. anti-human BD-4 500-P268bt Rabbit E, WB, NA 25μg 50μg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human BD-1 Catalogue # 900-K202Human BD-2 Catalogue # 900-K172Human BD-3 Catalogue # 900-K210Human BD-4 Catalogue # 900-K435Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human BD-1 Catalogue # 900-M202Human BD-2 Catalogue # 900-M172Human BD-4 Catalogue # 900-M435ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se19

www.peprotech.com2011-2012 CatalogueBDNF Brain-derived neurotrophic factorSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BDNF 450-02 E.coli 27.0 kDa 2µg 10µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman BDNF AF-450-02 E.coli 27.0 kDa N/A 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human BDNF 500-P84 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human BDNF 500-P84bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBBetacellulinSynonyms: BTCRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Betacellulin 100-50 E.coli 9.0 kDa 5µg 20µg 1mgMurine Betacellulin 315-21 E.coli 9.0 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Betacellulin 500-P254 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human Betacellulin 500-P254bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human Betacellulin Catalogue # 900-K372ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)20To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comBMP-2 Bone morphogenetic protein-2Synonyms: BMP-2ARecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BMP-2 120-02 E.coli 26.0 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human BMP-2 500-P195 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human BMP-2 500-P195bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human BMP-2 Catalogue # 900-K255ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)BBMP-3 Bone morphogenetic protein-3Synonyms: Osteogenin, BMP-3ARecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BMP-3 120-24B E.coli 25.2 kDa 10µg 50µg 1mgAdditional sizes available please inquireBMP-4 Bone morphogenetic protein-4Synonyms: BMP-2B, DVR4Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BMP-4 120-05 HeLa cells 31.0-36.0 kDa 1µg 5µg 1mgHuman BMP-4 120-05ET E.coli 23.8 kDa 2µg 10µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman BMP-4 AF-120-05ET E.coli 23.8 kDa N/A 10µg 1mgMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human BMP-4 500-M121 Mouse E, WB, N 500µgAdditional sizes available please inquireKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se21

www.peprotech.com2011-2012 CatalogueBMP-6 Bone morphogenetic protein-6Synonyms: VGRRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BMP-6 120-06 HEK 293 cells 30.0-36.0 kDa 2µg 10µg 1mgAdditional sizes available please inquireBMP-7 Bone morphogenetic protein-7Synonyms: OP-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BMP-7 120-03 CHO cells 28.8 kDa 2µg 10µg 1mgAdditional sizes available please inquireBPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human BMP-7 500-P198 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human BMP-7 500-P198bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human BMP-7 500-M123 Mouse E 500µgBMP-13/CDMP-2 Bone morphogenetic protein-13/CDMP-2Synonyms: Cartilage-derived morphogenetic protein-2, GDF-6Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BMP-13/CDMP-2 120-04 E.coli 27.0 kDa 10µg 50µg 1mgAdditional sizes available please inquire22To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comBRAK/CXCL14 Breast and kidney-expressed chemokineSynonyms: CXCL14, bolekineRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman BRAK 300-50 E.coli 9.4 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human BRAK 500-P237 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human BRAK 500-P237bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedHuman breast invasive ductal carcinoma (FFPE) stainedwith rabbit anti-human BRAK at 0.25 µg/mL.Staining of tumor cells in ducts.Tissue samples were provided by the Cooperative Human Tissue Network, whichis funded by the National Cancer Institute. Other investigators may have receivedspecimens from the same subjects.Photograph provided by Patsy Ruegg for PeproTech.B-CC10/CCL6Synonyms: CCL6, MRP-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BMurine C10 250-06 E.coli 10.7 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-murine C10 500-P112 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine C10 500-P112bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedCarboxypeptidaseSynonyms: Cpb1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BRat Carboxypeptidase 400-00 E.coli 35.1 kDa 5µg 25µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se23

www.peprotech.com2011-2012 CatalogueCardiotrophin-1Synonyms: CT-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Cardiotrophin-1 300-32 E.coli 21.5 kDa 2µg 10µg 1mgMurine Cardiotrophin-1 250-25 E.coli 21.3 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Cardiotrophin-1 500-P101 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human Cardiotrophin-1 500-P101bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedsCD14 soluble CD14CSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sCD14 110-01 HEK 293 cells 41.6 kDa 10μg 50μg 1mgAdditional sizes available please inquiresCD22 soluble CD22Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sCD22 100-01 CHO cells 75.0 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human sCD22 500-P227 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human sCD22 500-P227bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested24To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comsCD23 soluble CD23Synonyms: CD23 antigen, Fc-epsilon-RII, Lymphocyte IgE receptor, BLAST-2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sCD23 310-26 E.coli 19.2 kDa 5µg 20µg 1mgAdditional sizes available please inquiresCD27 Ligand soluble CD27 LigandSynonyms: TNFSF27, CD70, CD27LRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sCD27 Ligand/TNFSF27 310-30 CHO cells 18.8 kDa 10µg 50µg 1mgAdditional sizes available please inquireCsCD30 Ligand soluble CD30 LigandSynonyms: TNFSF8, CD153 antigen, CD30LRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sCD30 Ligand/TNFSF8 450-42 CHO cells 21.3 kDa 10µg 50µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se25

www.peprotech.com2011-2012 CataloguesCD40 Ligand soluble CD40 LigandSynonyms: TNFSF5, TRAP, CD154, gp39, T-BAMRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sCD40 Ligand 310-02 E.coli 16.3 kDa 10µg 50µg 1mgMurine sCD40 Ligand 315-15 E.coli 16.4 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human sCD40 Ligand 500-P142 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human sCD40 Ligand 500-P142bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human sCD40 Ligand 500-P142G Goat I, E, WB, NA 50µg 100µg 1mgBiot. anti-human sCD40 Ligand 500-P142Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedCELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human sCD40 Ligand Catalogue # 900-K145Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human sCD40 Ligand Catalogue # 900-M145ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Human cervical squamous cell carcinoma (FFPE) stainedwith goat anti-human sCD40L at 2.5 µg/ml.Tissue samples wereprovided by the Cooperative Human Tissue Network, which is funded by the NCI.Other investigators may have received specimens from the same subject.Photograph provided by Patsy Ruegg for Peprotech.sCD100Synonyms: Semaphorin-4D, BB18, A8, GR3Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sCD100 310-29 CHO cells 78.9 kDa 5µg 20µg 1mgAdditional sizes available please inquire26To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comCDNF Cerebral dopamine neurotrophic factorSynonyms: ARMETL1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman CDNF 450-05 E.coli 18.5 kDa 5µg 25µg 1mgAdditional sizes available please inquireChemerinSynonyms: Tazaroyene-induced gene 2 (TIG2), RARRES2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Chemerin 300-66 E.coli 15.6 kDa 5µg 25µg 1mgAdditional sizes available please inquireCNTF Ciliary neurotrophic factorSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman CNTF 450-13 E.coli 22.7 kDa 5µg 20µg 1mgRat CNTF 450-50 E.coli 22.7 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human CNTF 500-P140 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human CNTF 500-P140bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat CNTF 500-P79 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat CNTF 500-P79bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human CNTF Catalogue # 900-K158CELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se27

www.peprotech.com2011-2012 CatalogueCTACK/CCL27 Cuteaneous T-cell attracting chemokineSynonyms: CCL27, ALP, Skinkine, EskineRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman CTACK 300-54 E.coli 9.8 kDa 5µg 20µg 1mgMurine CTACK 250-26 E.coli 10.9 kDa 5µg 20µg 1mgAdditional sizes available please inquireCCTGF Connective tissue growth factorSynonyms: CCN2, Hypertrophic chondrocyte-specific protein 24 (HCS24)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman CTGF (98 a.a.) 120-19 E.coli 11.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human CTGF 500-P252 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human CTGF 500-P252bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human CTGF Catalogue # 900-K317ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)CTGFL/WISP-2 Connective tissue growth factor-like proteinSynonyms: CCN5, CT-58Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman CTGFL/WISP-2 120-16 E.coli 24.8 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human CTGFL/WISP-2 500-P212 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human CTGFL/WISP-2 500-P212bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I= IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested28To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comCTLA-4Polyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-murine CTLA-4 500-P147 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine CTLA-4 500-P147bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedCXCL16Synonyms: SRPSOXRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman CXCL16 300-55 E.coli 10.1 kDa 5µg 25µg 1mgMurine CXCL16 250-28 E.coli 9.9 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human CXCL16 500-P200 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human CXCL16 500-P200bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine CXCL16 500-P201G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-murine CXCL16 500-P201Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedCELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human CXCL16 Catalogue # 900-K230Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human CXCL16 Catalogue # 900-M230ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)CYR61Synonyms: CNN1, GIGI proteinRecombinant ProteinsProduct Catalogue # Source MW SIZE A SIZE BHuman CYR61 120-25 E.coli 39.5 kDa 5µg 20µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se29

www.peprotech.com2011-2012 CatalogueDDAHSynonyms: Dimethylarginine dimethylaminohydrolase-1, Dimethylargininase-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman DDAH-1 150-13 E. coli 31.4 kDa 10μg 50μg 1mgAdditional sizes available please inquireDKK-1Synonyms: Dickkopf-related protein-1, Dickkopf-1, SKRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman DKK-1 120-30 HEK 293 cells 35.0-40.0 kDa 2μg 10μg 1mgAdditional sizes available please inquireDsDLL-1Synonyms: Delta-like protein-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sDLL-1 140-08 HEK 293 cells 56.0-60.0 kDa 5μg 25μg 1mgAdditional sizes available please inquiresDLL-4Synonyms: Delta-like protein-4, Drosophila Delta homolog 4Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sDLL-4 140-07 HEK 293 cells 54.0 kDa 5μg 25μg 1mgAdditional sizes available please inquire30To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comEGF Epidermal growth factorSynonyms: Urogastrone, URGRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman EGF AF-100-15 E.coli 6.2 kDa 100µg 500µg 1mgMurine EGF 315-09 E.coli 6.0 kDa 100µg 500µg 1mgRat EGF 400-25 E.coli 6.2 kDa 20µg 100µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman EGF AF-100-15 E.coli 6.2 kDa N/A 500µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human EGF 500-P45 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human EGF 500-P45bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine EGF 500-P174G Goat E, WB, N 50µg 100µg 1mgBiot. anti-murine EGF 500-P174Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-rat EGF 500-P277 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat EGF 500-P277bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedEMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-murine EGF 500-M136 Rat E 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human EGF Catalogue # 900-K05Murine EGF Catalogue # 900-K179Rat EGF Catalogue # 900-K390Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human EGF Catalogue # 900-M05Rat EGF Catalogue # 900-M390ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Normal human skin (FFPE) stained withrabbit anti-human EGF at 1.0 µg/ml. Stainingof apocrine gland epithelial cells andsecretory cells. Photograph by PeproTech.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se31

www.peprotech.com2011-2012 CatalogueEGF ReceptorSynonyms: ErbB1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman EGF Receptor 100-15R CHO cells 68.5 kDa 2μg 10μg 1mgAdditional sizes available please inquireEG-VEGF Endocrine gland-derived endothelial growth factorSynonyms: Prokineticin, PROK1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman EG-VEGF 100-44 E.coli 9.6 kDa 5µg 20µg 1mgAdditional sizes available please inquireEPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human EG-VEGF 500-P188G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human EG-VEGF 500-P188Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human EG-VEGF Catalogue # 900-K244ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)EMAP-II Endothelial-monocyte activating polypeptide IIPolyclonal Antibodies - Antigen Affinity Purified Size A Size BProduct Catalogue # Source APPLICATIONS $195.00 $250.00 1mgAnti-human EMAP-II 500-P172G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human EMAP-II 500-P172Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested32To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comENA-78/LIX (CXCL5) Epithelial neutrophil activating peptide-78Synonyms: CXCL5, LIX (murine)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman ENA-78 (74 a.a.) 300-22 E.coli 8.0 kDa 5µg 20µg 1mgHuman ENA-78 (71 a.a.) 300-22B E.coli 8.0 kDa 5µg 20µg 1mgMurine LIX (93 a.a.) 250-17 E.coli 9.8 kDa 5µg 20µg 1mgMurine LIX (70 a.a.) 250-36 E.coli 7.6 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human ENA-78 500-P91 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human ENA-78 500-P91bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine LIX 500-P146 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine LIX 500-P146bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedEndostatinESynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Endostatin 150-01 E.coli 20.2 kDa 20µg 100µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Endostatin 500-P262 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human Endostatin 500-P262bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedAnti-Human Endostatin (Catalogue #500-P262) *Human breast invasive ductal carcinoma (FFPE)stained with Rabbit Anti-Human Endostatin at 0.25µg/mL. Staining of endothelial cells.Tissue samples were provided by the Cooperative Human Tissue Network,which is funded by the National Cancer Institute. Other investigators mayhave received specimens from the same subjects.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se33

www.peprotech.com2011-2012 CatalogueEnterokinaseSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Enterokinase 450-48C CHO cells 108.7 kDa 10µg 50µg 1mgAdditional sizes available please inquireEotaxin/CCL11Synonyms: CCL11ERecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Eotaxin 300-21 E.coli 8.3 kDa 5µg 20µg 1mgMurine Eotaxin 250-01 E.coli 8.4 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Eotaxin 500-P41 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human Eotaxin 500-P41bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human Eotaxin 500-P41G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human Eotaxin 500-P41Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine Eotaxin 500-P67 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine Eotaxin 500-P67bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human Eotaxin 500-M25 Mouse E, WB 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human Eotaxin Catalogue # 900-K69Murine Eotaxin Catalogue # 900-K68ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Chemotaxis analysis of human Eotaxin.Data shows the migration of purified humaneosinophils in response to human Eotaxin.34To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comEotaxin-2/CCL24Synonyms: CCL24, MPIF-2, CkB-6Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Eotaxin-2 300-33 E.coli 8.8 kDa 5µg 20µg 1mgMurine Eotaxin-2 250-22 E.coli 10.3 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Eotaxin-2 500-P103G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human Eotaxin-2 500-P103Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine Eotaxin-2 500-P175G Goat E, WB, N 50µg 100µg 1mgBiot. anti-murine Eotaxin-2 500-P175Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human Eotaxin-2 500-M31 Mouse E, WB 500µgEotaxin-3/CCL26ESynonyms: CCL26Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Eotaxin-3 300-48 E.coli 8.4 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Eotaxin-3 500-P156G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human Eotaxin-3 500-P156Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human Eotaxin-3 500-M32 Mouse E, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human Eotaxin-3 Catalogue # 900-K167ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se35

www.peprotech.com2011-2012 CatalogueEpigenSynonyms: EPG, Epithelial mitogenRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Epigen 100-51 E.coli 7.9 kDa 5µg 25µg 1mgAdditional sizes available please inquireEpiregulinSynonyms: EREGRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Epiregulin 100-04 E.coli 5.6 kDa 5µg 25µg 1mgAdditional sizes available please inquireEBioassay analysis of humanEpiregulin (proliferation) usingmouse Balb/3T3 cells.E-SelectinSynonyms: Endothelial leukocyte adhesion molecule-1 (ELAM1), CD62E, SELERecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman E-Selectin 150-15 CHO cells 58.6 kDa 10µg 50µg 1mgAdditional sizes available please inquire36To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comExodus-2/CCL21Synonyms: CCL21, 6Ckine, SLCRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Exodus-2 300-35 E.coli 12.2 kDa 5µg 20µg 1mgMurine Exodus-2 250-13 E.coli 12.0 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Exodus-2 500-P109 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human Exodus-2 500-P109bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine Exodus-2 500-P114 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine Exodus-2 500-P114bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedAnti-Human Exodus-2(Catalogue #500-P109)Human prostate malignant adenocarcinoma(FFPE) stained withRabbit Anti-Human Exodus-2 at0.75 µg/mL.Tissue samples were provided by the CooperativeHuman Tissue Network, which is funded by theNational Cancer Institute. Other investigators mayhave received specimens from the same subjects.EBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se37

www.peprotech.com2011-2012 CataloguesFas Ligand soluble Fas LigandSynonyms: TNFSF6, sFasL, CD95L, Apo I Ligand, APTLRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sFas Ligand 310-03H CHO cells 17.9 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human sFas Ligand 500-P184G Goat I, E, WB, N 50µg 100µg 1mgBiot. anti-human sFas Ligand 500-P184Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedFHuman angiosarcoma (FFPE) stained with goatanti-human sFas Ligand at 2.5 µg/mL. Photographprovided by Patsy Ruegg for PeproTech.sFas Receptor soluble Fas receptorSynonyms: TNFRSF6, CD95, Fas antigen, Apo IRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sFas receptor 310-20 E.coli 17.6 kDa 5µg 20µg 1mgAdditional sizes available please inquireBioassay analysis of human sFasReceptor. Determined by its abilityto inhibit sFas Ligand inducedcytotoxicity using Jurkat cells.38To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comFGF Fibroblast growth factorSynonyms: See FGF chart on pages 143-144.Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman FGF-acidic 100-17A E.coli 15.8 kDa 10µg 50µg 1mgMurine FGF-acidic 450-33A E.coli 15.9 kDa 10µg 50µg 1mgHuman FGF-basic 100-18B E.coli 17.2 kDa 10µg 50µg 1mgMurine FGF-basic 450-33 E.coli 16.2 kDa 10µg 50µg 1mgRat FGF-basic 400-29 E.coli 16.3 kDa 10µg 50µg 1mgHuman FGF-4 100-31 E.coli 19.7 kDa 5µg 25µg 1mgHuman FGF-5 100-34 E.coli 27.6 kDa 10µg 50µg 1mgHuman FGF-6 100-30 E.coli 18.7 kDa 5µg 25µg 1mgHuman FGF-7/KGF 100-19 E.coli 18.9 kDa 2µg 10µg 1mgHuman FGF-8 100-25 E.coli 22.4 kDa 5µg 25µg 1mgHuman FGF-9 100-23 E.coli 23.4 kDa 5µg 20µg 1mgMurine FGF-9 450-30 E.coli 23.3 kDa 2µg 10µg 1mgHuman FGF-10 100-26 E.coli 19.3 kDa 5µg 25µg 1mgHuman FGF-16 100-29 E.coli 23.7 kDa 5µg 25µg 1mgHuman FGF-17 100-27 E.coli 22.7 kDa 5µg 25µg 1mgHuman FGF-18 100-28 E.coli 20.1 kDa 5µg 25µg 1mgHuman FGF-19 100-32 E.coli 21.8 kDa 5µg 25µg 1mgHuman FGF-20 100-41 E.coli 23.2 kDa 3µg 15µg 1mgHuman FGF-21 100-42 E.coli 19.5 kDa 5µg 25µg 1mgHuman FGF-23 100-52 E.coli 25.4 kDa 5μg 20μg 1mgAdditional sizes available please inquireFAnimal-Free Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman FGF-acidic AF-100-17A E.coli 15.8 kDa N/A 50µg 1mgHuman FGF-basic AF-100-18B E.coli 17.2 kDa N/A 50µg 1mgHuman FGF-7/KGF AF-100-19 E.coli 18.9 kDa N/A 10µg 1mgAdditional sizes available please inquireFGF continued on next page...Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se39

www.peprotech.com2011-2012 CatalogueFGF continued...FPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human FGF-acidic 500-P17 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human FGF-acidic 500-P17bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human FGF-basic 500-P18 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human FGF-basic 500-P18bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human FGF-4 500-P158 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human FGF-4 500-P158bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human FGF-7/KGF 500-P19 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human FGF-7/KGF 500-P19bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine FGF-9 500-P66 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine FGF-9 500-P66bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human FGF-10 500-P151G Goat I, E, WB, NA 50µg 100µg 1mgBiot. anti-human FGF-10 500-P151Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-human FGF-16 500-P160G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human FGF-16 500-P160Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-human FGF-17 500-P152 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human FGF-17 500-P152bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human FGF-17 500-P152G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human FGF-17 500-P152Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human FGF-basic 500-M38 Mouse I, E, WB 500µgAnti-human FGF-5 500-M40 Mouse E, N 500µgKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human FGF-basic Catalogue # 900-K08Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human FGF-basic Catalogue # 900-M08ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)FGF continued on next page...40To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comFGF continued...Human carcinoid tissue (FFPE) stained withgoat anti-human FGF-10 at 1.0 µg/ml.Tissue samples were provided by the Cooperative HumanTissue Network, which is funded by the NCI. Otherinvestigators may have received specimens from the samesubject. Photograph provided by Patsy Ruegg for PeproTech.Normal skin (FFPE) stained with Rabbit antihumanKGF/FGF-7 at 0.5 µg/ml. Staining ofgland epithelial cells. Photograph by Peprotech.FHuman breast invasive ductal carcinoma(FFPE) stained with rabbit anti-humanFGF-basic at 0.25 µg/ml.Tissue samples were provided by the Cooperative HumanTissue Network, which is funded by the NCI. Otherinvestigators may have received specimens from the samesubject. Photograph by PeproTech.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se41

www.peprotech.com2011-2012 Catalogueflt3-LigandSynonyms: flt3L, Fms-related tyrosine kinase 3 ligandRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman flt3-Ligand 300-19 E.coli 17.6 kDa 2µg 10µg 1mgMurine flt3-Ligand 250-31L E.coli 18.6 kDa 2µg 10µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman flt3-Ligand AF-300-19 E.coli 17.6 kDa N/A 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human flt3-Ligand 500-P42 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human flt3-Ligand 500-P42bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedFFollistatinSynonyms: FS, activins-binding proteinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Follistatin 120-13 E.coli 31.5 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Follistatin 500-P207 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human Follistatin 500-P207bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human Follistatin Catalogue # 900-K299ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)42To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comFractalkine/CX3CL1Synonyms: CX3CL1, NeurotactinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Fractalkine 300-31 E.coli 8.5 kDa 5µg 20µg 1mgRat Fractalkine 400-26 E.coli 8.7 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Fractalkine 500-P98 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human Fractalkine 500-P98bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedsFRP-1 soluble Frizzled Related Protein-1Synonyms: Secreted Apoptosis Related Protein-2, SARP-2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sFRP-1 120-29 HeLa cells 31.0-36.0 kDa 5µg 20µg 1mgAdditional sizes available please inquireF-GgAcrp30Synonyms: Adipolean (human)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman gAcrp30/Adipolean 450-21 E.coli 16.6 kDa 5µg 25µg 1mgMurine gAcrp30 450-27 E.coli 16.6 kDa 5µg 25µg 1mgHuman gAcrp30/Adipolean Variant 450-20 E.coli 18.1 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human gAcrp30/Adipolean 500-P193G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human gAcrp30/Adipolean 500-P193Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-human Adipolean Variant 500-P205 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human Adipolean Variant 500-P205bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se43

www.peprotech.com2011-2012 CatalogueGalectin-1Synonyms: beta-galactoside-binding lectin L-14-I, Galaptin, 14 kDa lectin, S-LAC lectin 1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Galectin-1 450-39 E.coli 14.5 kDa 10µg 50µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Galectin-1 500-P210 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human Galectin-1 500-P210bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedGGalectin-3Synonyms: Galactose-specific lectin-3, IgE-binding protein, MAC2, L-29, CPB-35Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Galectin-3 450-38 E.coli 26.0 kDa 10µg 50µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Galectin-3 500-P246 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human Galectin-3 500-P246bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedHuman pancreas ductal adenocarcinoma(FFPE) stained with rabbit anti-humanGalectin-3 at 31.25 ng/mL. Staining ofnerve cells and tumor cells. (photo.1, left)Staining of tumor cells. (photo.2, right)Tissue samples were provided by the Cooperative HumanTissue Network, which is funded by the NationalCancer Institute. Other investigators may have receivedspecimens from the same subjects.Photograph provided byPatsy Ruegg for PeproTech.44To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comGCP-2/CXCL6 Granulocyte chemotactic protein-2Synonyms: CXCL6Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GCP-2 300-41 E.coli 7.9 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human GCP-2 500-P120 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human GCP-2 500-P120bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedkDa148Representative Western Transfer (Blot) analysis ofhuman GCP-2 using Rabbit anti-human GCP-2at 0.20 µg/ml.60423022176G4Human GCP-2 induceschemotaxis of human neutrophils.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se45

www.peprotech.com2011-2012 CatalogueG-CSF Granulocyte colony-stimulating factorSynonyms: CSF-3, MGI-1G, GM-CSFβ, pluripoietinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman G-CSF 300-23 E.coli 18.7 kDa 2µg 10µg 1mgMurine G-CSF 250-05 E.coli 19.0 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human G-CSF 500-P43 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human G-CSF 500-P43bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine G-CSF 500-P69 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine G-CSF 500-P69bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedGELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human G-CSF Catalogue # 900-K77Murine G-CSF Catalogue # 900-K103ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bioassay analysis of murine G-CSF (proliferation)using mouse M-NFS-60 cells.GDF-2 Growth and differentiation factor-2Synonyms: Growth/Differentiation Factor-2, BMP-9Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GDF-2 120-07 CHO cells 24.1 kDa 2µg 10µg 1mgAdditional sizes available please inquire46To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comGDF-3 Growth and differentiation factor-3Synonyms: UNQ222/PRO248Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GDF-3 120-22 E.coli 26.0 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human GDF-3 500-P235 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human GDF-3 500-P235bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedGDF-5/CDMP-1 Growth and differentiation factor-5Synonyms: BMP-14, Cartilage-Derived Morphogenetic Protein-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GDF-5/CDMP-1 120-01 E.coli 27.0 kDa 10µg 50µg 1mgAdditional sizes available please inquireGGDF-11 Growth and differentiation factor-11Synonyms: BMP-11Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GDF-11 120-11 E.coli 25.0 kDa 5µg 20µg 1mgAdditional sizes available please inquireGDF-15/MIC-1 Growth and differentiation factor-15Synonyms: Placental TGF-β, Prostate differentiation factorRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GDF-15/MIC-1 120-28 cell culture 24.5 kDa 5µg 20µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se47

www.peprotech.com2011-2012 CatalogueGDNF Glial-derived neurotrophic factorSynonyms: ATF-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GDNF 450-10 E.coli 30.4 kDa 2µg 10µg 1mgMurine GDNF 450-44 E.coli 30.2 kDa 2µg 10µg 1mgRat GDNF 450-51 E.coli 30.0 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human GDNF 500-P81 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human GDNF 500-P81bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedkDaGRepresentative Western Transfer (Blot) analysis ofhuman GDNF using Rabbit anti-human GDNFat 0.20µg/ml.148604230221764GLP-1 Glucagon-like peptideSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GLP-1 (7-36 a.a.) 130-08 E.coli 3.3 kDa 200µg 1000µg 5mgAdditional sizes available please inquireGlu-CSynonyms: V8 ProteaseRecombinant ProteinsProduct Catalogue # Source MW Size A Size BStaphylococcus Glu-C 450-46 E.coli 29.1 kDa 50µg 250µg 1mgAdditional sizes available please inquire48To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comGM-CSF Granulocyte/macrophage colony stimulating factorSynonyms: CSF-2, Pluripoietin-α, MGI-1GMRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GM-CSF 300-03 E.coli 14.6 kDa 5µg 20µg 1mgMurine GM-CSF 315-03 E.coli 14.2 kDa 5µg 20µg 1mgRat GM-CSF 400-23 E.coli 14.5 kDa 5µg 20µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman GM-CSF AF-300-03 E.coli 14.6 kDa N/A 20µg 1mgMurine GM-CSF AF-315-03 E.coli 14.2 kDa N/A 20µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human GM-CSF 500-P33 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human GM-CSF 500-P33bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine GM-CSF 500-P65 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine GM-CSF 500-P65bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedGELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells..Human GM-CSF Catalogue # 900-K30Murine GM-CSF Catalogue # 900-K55ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Standard curve using Murine GM-CSF ELISAdevelopment kit.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se49

www.peprotech.com2011-2012 CatalogueGMF-β Glial Maturation Factor-betaSynonyms: GMFβRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GMF-β 450-37 E.coli 16.5 kDa 2µg 10µg 1mgAdditional sizes available please inquireGranzyme BSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BMurine Granzyme B 140-03 Baculovirus 28.9 kDa 2µg 10µg 1mgAdditional sizes available please inquireG50To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comGROα/MGSA, GROβ, GROγ Growth regulated proteinSynonyms: GROα: CXCL1, GRO1, MGSAα, KC, NAP-3, CINCGROβ: CXCL2, GRO2, MGSAβ, MIP-2αGROγ: CXCL3, GRO3, MIP-2βRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman GRO/MGSA (CXCL1) 300-11 E.coli 7.8 kDa 5µg 25µg 1mgMurine KC (CXCL1) 250-11 E.coli 7.8 kDa 5µg 20µg 1mgRat GRO/KC (CXCL1) 400-10 E.coli 7.8 kDa 5µg 25µg 1mgHuman GROβ (CXCL2) 300-39 E.coli 7.9 kDa 2µg 10µg 1mgMurine MIP-2 (CXCL2) 250-15 E.coli 7.8 kDa 5µg 20µg 1mgRat GROβ/MIP-2 (CXCL2) 400-11 E.coli 7.9 kDa 5µg 25µg 1mgHuman GROγ (CXCL3) 300-40 E.coli 7.9 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human GRO/MGSA 500-P92 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human GRO/MGSA 500-P92bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine KC 500-P115 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine KC 500-P115bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat GRO/KC 500-P74 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat GRO/KC 500-P74bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human GROβ 500-P104 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human GROβ 500-P104bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine MIP-2 500-P130 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine MIP-2 500-P130bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat GROβ/MIP-2 500-P75 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat GROβ/MIP-2 500-P75bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human GROγ 500-P105 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human GROγ 500-P105bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedGELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human GRO/MGSA Catalogue # 900-K38Human GROβ Catalogue # 900-K120Rat GRO/KC Catalogue # 900-K57Murine MIP-2 Catalogue # 900-K152Murine KC Catalogue # 900-K127Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Murine KC Catalogue # 900-M127ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se51

www.peprotech.com2011-2012 CatalogueHB-EGF Heparin-binding EGF-like growth factorSynonyms: HBEGF, Diphtheria toxin receptor, DT-RRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman HB-EGF 100-47 E.coli 9.8 kDa 10µg 50µg 1mgAdditional sizes available please inquireHCC-1/CCL14 Hemofiltrate CC chemokine-1Synonyms: CCL14, HCC-3, NCC-2HRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman HCC-1 (72 a.a.) 300-38 E.coli 8.4 kDa 2µg 10µg 1mgHuman HCC-1 (66 a.a.) 300-38B E.coli 7.7 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human HCC-1 500-P106 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human HCC-1 500-P106bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedHuman HCC-1 induceschemotaxis of humanmonocytes.52To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comHeregulinβ-1Synonyms: HRG, Neuregulin1 (NRG), Breast cancer cell differentiation factor p45, ARIA (Acetylcholinereceptor inducing activity), glial factor, Neu differentiation factor (rat)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Heregulinβ-1 100-03 E.coli 7.5 kDa 10µg 50µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman Heregulinβ-1 AF-100-03 E.coli 7.5 kDa N/A 50µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Heregulinβ-1 500-P288 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human Heregulinβ-1 500-P288bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedHSDS-PAGE gel analysis under non-reducing conditionsfor human HCC-1 (Lane 1), HB-EGF (Lane 2),and Heregulinβ-1 (Lane 3), markers (Lane 4).HGF Hepatocyte growth factorSynonyms: Scatter factor (SF), Hepatopoietin (HPTA)Recombinant ProteinsProduct Catalogue # Source MW Size A Size B

www.peprotech.com2011-2012 CatalogueHVEM-FcSynonyms: TNFSFR14, HVEM, ATAR, TR2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman HVEM-Fc 310-27 Insect cells 88.5 kDa 20µg 100µg 1mgAdditional sizes available please inquireSDS-PAGE gel analysis under non-reducing conditions forhuman HGF(Lane 1), HVEM-Fc (Lane 2), and markers(Lane 3). Due to glycosylation HVEM-Fc migrates higher.

PEPROTECH ®www.peprotech.comICAM-1 Intercellular adhesion molecule-1Synonyms: Major group rhinovirus receptor, CD54 antigenRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman ICAM-1 150-05 CHO cells 49.5 kDa 10μg 50μg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human ICAM-1 500-P287 Rabbit I, E, WB, NA 50μg 100μg 1mgBiot. anti-human ICAM-1 500-P287Bbt Rabbit E, WB, NA 25μg 50μg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human ICAM-1 Catalogue # 900-K464Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human ICAM-1 Catalogue # 900-M464ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)IIFN-a Interferon alphaSynonyms: Interferon alpha, Leukocyte interferon, B cell interferon, Type I interferonRecombinant ProteinsProduct CatalogUE # Source MW Size A Size BHuman IFN-a 300-02A E.coli 19.2 kDa 20μg 100μg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct CatalogUE # Source APPLICATIONS Size A Size BAnti-human IFN-a 500-P32AG Goat E, WB, N 50μg 100μg 1mgBiot. anti-human IFN-a 500-P32Abt Rabbit E, WB, NA 25μg 50μg 1mgBiot. = BiotinylatedKey: E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIFN-β Interferon betaSynonyms: Leukocyte interferon, B cell interferon, Type I interferonRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IFN-β 300-02BC CHO cells 20.0-22.4 kDa 5μg 20μg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IFN-β 500-P32B Rabbit E, WB, N 50μg 100μg 1mgBiot. anti-human IFN-β 500-P32Bbt Rabbit E, WB, NA 25μg 50μg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se55

www.peprotech.com2011-2012 CatalogueIFN-γ Interferon-gammaSynonyms: Immune Interferon, type II interferon, T cell interferon, MAFRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IFN-γ 300-02 E.coli 16.7 kDa 20µg 100µg 1mgMurine IFN-γ 315-05 E.coli 15.6 kDa 20µg 100µg 1mgRat IFN-γ 400-20 E.coli 15.6 kDa 20µg 100µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IFN-γ AF-300-02 E.coli 16.7 kDa N/A 100µg 1mgAdditional sizes available please inquireIPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IFN-γ 500-P32 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IFN-γ 500-P32bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IFN-γ 500-P119 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IFN-γ 500-P119bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IFN-γ 500-P122 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-rat IFN-γ 500-P122bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IFN-γ 500-P122G Goat E, WB, N 50µg 100µg 1mgBiot. anti-rat IFN-γ 500-P122Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IFN-γ 500-M90 Mouse E, WB, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IFN-γ Catalogue # 900-K27Murine IFN-γ Catalogue # 900-K98Rat IFN-γ Catalogue # 900-K109Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IFN-γ Catalogue # 900-M27Murine IFN-γ Catalogue # 900-M98ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Continued on next page56To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIFN-γ ContinuedHuman cervical squamous cellcarcinoma (FFPE) stainedwith rabbit anti-human IFNγat 0.25 µg/mL.Tissue samples were provided by theCooperative Human Tissue Network,which is funded by the National CancerInstitute. Other investigators mayhave received specimens from the samesubjects.Photograph provided by PatsyRuegg for PeproTech.IFN-λ -1,-2 Interferon lambda-1,-2Synonyms: IFN-λ1: IL-29; IFN-λ2: IL-28ARecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IFN-λ1 300-02L E.coli 19.8 kDa 5µg 20µg 1mgHuman IFN-λ2 300-02K E.coli 19.6 kDa 5µg 20µg 1mgMurine IFN-λ2 250-33 E.coli 19.8 kDa 5µg 20µg 1mgAdditional sizes available please inquireIPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IFN-λ2 500-P247 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human IFN-λ2 500-P247bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIFN-W Interferon-omegaSynonyms: IFN alpha II-1, IFNW1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IFN-W 300-02J E.coli 19.9 kDa 20µg 100µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se57

www.peprotech.com2011-2012 CatalogueIGF-I and IGF-II Insulin-like growth factor I and IISynonyms: IGF-I: Somatamedin C, IGF-IA; IGF-II: Somatamedin ARecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IGF-I 100-11 E.coli 7.6 kDa 20µg 100µg 1mgMurine IGF-I 250-19 E.coli 7.6 kDa 10µg 50µg 1mgHuman IGF-II 100-12 E.coli 7.5 kDa 10µg 50µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IGF-I AF-100-11 E.coli 7.6 kDa N/A 100µg 1mgAdditional sizes available please inquireIPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IGF-I 500-P11 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IGF-I 500-P11bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IGF-I 500-P157G Goat E, WB, N 50µg 100µg 1mgBiot. anti-murine IGF-I 500-P157Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-human IGF-II 500-P12 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IGF-II 500-P12bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Murine IGF-I Catalogue # 900-K170ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Normal human placenta (FFPE) stained with rabbitanti-human IGF-II at 5.0 µg/ml. Staining of epithelialcells and vessel lining cells. Photograph by PeproTech.58To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIGF-BP Insulin-like growth factor binding proteinsSynonyms: IGF-BP1: IBP-1, Placenta Protein 12 (PP12)IGF-BP2: IBP-2IGF-BP3: Growth-hormone-dependant binding proteinIGF-BP4: IBP-4, HT29-IGF-BP, colon cancer cell growth inhibitorIGF-BP5: noneIGF-BP6: IBP-6IGF-BP7: Mac25, IGFBPrP1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IGF-BP1 350-10 E.coli 25.4 kDa 5µg 25µg 1mgHuman IGF-BP2 350-06B Insect cells 31.5 kDa 5µg 20µg 1mgHuman IGF-BP3 100-08 E.coli 28.8 kDa 5µg 25µg 1mgHuman IGF-BP4 350-05B Insect cells 26.0 kDa 5µg 20µg 1mgHuman IGF-BP5 100-05 E.coli 28.6 kDa 5µg 25µg 1mgHuman IGF-BP6 350-07B Insect cells 23.0-30.0 kDa 5µg 20µg 1mgHuman IGF-BP7 350-09 E.coli 26.4 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IGF-BP1 500-P228 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human IGF-BP1 500-P228bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human IGF-BP3 500-P230 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human IGF-BP3 500-P230bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human IGF-BP5 500-P232 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human IGF-BP5 500-P232bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human IGF-BP7 500-P234 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human IGF-BP7 500-P234bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedINormal human placenta (FFPE) stained with rabbitanti-human IGF-BP3 at 2.5 µg/ml. Photographby PeproTech.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se59

www.peprotech.com2011-2012 CatalogueIL-1α Interleukin-1 alphaISynonyms: Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyteendogenous mediator (LEM), Mononuclear cell factor (MCF)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-1α 200-01A E.coli 18.0 kDa 2µg 10µg 1mgMurine IL-1α 211-11A E.coli 17.9 kDa 2µg 10µg 1mgRat IL-1α 400-01A E.coli 17.7 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-1α 500-P21A Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-1α 500-P21Abt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-1α 500-P51A Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-1α 500-P51Abt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IL-1α 500-P180G Goat E, WB, N 50µg 100µg 1mgBiot. anti-rat IL-1α 500-P180Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-1α Catalogue # 900-K11Murine IL-1α Catalogue # 900-K82Rat IL-1α Catalogue # 900-K204ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)1.701.601.50OD (490 nm)1.401.301.201.101.000.900.800.00 0.01 0.10 1.00 10.00 100.00 1000.00Human IL-1aRat IL-1aMurine IL-1aIL-1a (pg/ml) [log scale]Bioassay analysis of human, murine, and rat IL-1α (proliferation)using mouse D10S cells.60To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-1β Interleukin-1 betaSynonyms: Catabolin, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyteendogenous mediator (LEM), Mononuclear cell factor (MCF)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-1β 200-01B E.coli 17.3 kDa 2µg 10µg 1mgMurine IL-1β 211-11B E.coli 17.5 kDa 2µg 10µg 1mgRat IL-1β 400-01B E.coli 17.3 kDa 2µg 10µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IL-1β AF-200-01B E.coli 17.3 kDa N/A 10µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-murine IL-1β 500-P51 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-1β 500-P51bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IL-1β 500-P80 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat IL-1β 500-P80bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Murine IL-1β Catalogue # 900-K47Rat IL-1β Catalogue # 900-K91Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Murine IL-1β Catalogue # 900-M47ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Standard curve using Rat IL-1β ELISA development kit.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se61

www.peprotech.com2011-2012 CatalogueIL-1RA Interleukin-1 receptor antagonistSynonyms: ICIL-1RA, IRAP, IL-1RNRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-1RA 200-01RA E.coli 17.2 kDa 20µg 100µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-1RA 500-P209 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-1RA 500-P209bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-1RA Catalogue # 900-K474IMini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-1RA Catalogue # 900-M474ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Standard curve usingHuman IL-1RA ELISAdevelopment Kit.62To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-2 Interleukin-2Synonyms: T-cell growth factor (TCGF), AldesleukinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-2 200-02 E.coli 15.4 kDa 10µg 50µg 1mgMurine IL-2 212-12 E.coli 17.2 kDa 5µg 20µg 1mgRat IL-2 400-02 E.coli 15.3 kDa 5µg 20µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IL-2 AF-200-02 E.coli 15.4 kDa N/A 50µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-2 500-P22 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-2 500-P22bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human IL-2 500-P22G Goat I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-2 500-P22Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine IL-2 500-P111 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-2 500-P111bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IL-2 500-P274 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat IL-2 500-P274bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IL-2 500-M02 Mouse I, E 500µgAnti-murine IL-2 500-M127 Rat E 500µgAnti-rat IL-2 500-M129 Mouse E 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-2 Catalogue # 900-K12Murine IL-2 Catalogue # 900-K108Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-2 Catalogue # 900-M12ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Human breast invasive ductal carcinoma (FFPE) stainedwith goat anti-human IL-2 at 0.5 µg/mL. Staining of lymphocyteswith tumor cells in the center.Tissue samples were provided by the Cooperative Human TissueNetwork, which is funded by the National Cancer Institute. Otherinvestigators may have received specimens from the same subjects.Photograph provided by Patsy Ruegg for PeproTech.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se63

www.peprotech.com2011-2012 CataloguesIL-2Ra soluble Interleukin-2 receptor alphaSynonyms: TAC-antigen, CD25 antigenRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sIL-2Ra 200-02R Insect cells 31.0 kDa 5µg 25µg 1mgMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAdditional sizes available please inquireAnti-human sIL-2Ra 500-M02R Mouse I, E 500µgKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIL-3 Interleukin-3Synonyms: Mast cell growth factor (MCGF), multipotential colony-stimulating factor (Multi-CSF),hematopoietic cell growth factor (HCGF), P-cell stimulation factorIRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-3 200-03 E.coli 15.0 kDa 2µg 10µg 1mgMurine IL-3 213-13 E.coli 15.1 kDa 2µg 10µg 1mgRat IL-3β 400-03 E.coli 16.3 kDa 5µg 20µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IL-3 AF-200-03 E.coli 15.0 kDa N/A 10µg 1mgMurine IL-3 AF-213-13 E.coli 15.1 kDa N/A 10µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-3 500-P23 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-3 500-P23bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-3 500-P53 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-3 500-P53bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IL-3β 500-P177G Goat E, WB, N 50µg 100µg 1mgBiot. anti-rat IL-3β 500-P177Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-3 Catalogue # 900-K13Murine IL-3 Catalogue # 900-K48ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)64To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-4 Interleukin-4Synonyms: BCGF, BCDF, B cell stimulating factor (BSF-1)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-4 200-04 E.coli 14.9 kDa 5µg 20µg 1mgMurine IL-4 214-14 E.coli 13.5 kDa 5µg 20µg 1mgRat IL-4 400-04 E.coli 14.0 kDa 5µg 20µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IL-4 AF-200-04 E.coli 14.9 kDa N/A 20µg 1mgMurine IL-4 AF-214-14 E.coli 13.5 kDa N/A 20µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-4 500-P24 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-4 500-P24bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-4 500-P54 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-4 500-P54bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IL-4 500-P94 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-rat IL-4 500-P94bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I= IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IL-4 500-M04 Mouse E, WB, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-4 Catalogue # 900-K14Murine IL-4 Catalogue # 900-K49Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-4 Catalogue # 900-M14ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se65

www.peprotech.com2011-2012 CataloguesIL-4Rα soluble Interleukin-4 receptor alphaSynonyms: CD124Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sIL-4Rα 200-04R HEK 293 cells 23.7 kDa 3µg 15µg 1mgAdditional sizes available please inquireIL-5 Interleukin-5Synonyms: EDF, BCDFII, TRFIRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-5 200-05 E.coli 26.0 kDa 2µg 10µg 1mgMurine IL-5 215-15 E.coli 26.2 kDa 5µg 25µg 1mgRat IL-5 400-05 E.coli 26.0 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-5 500-P25 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-5 500-P25bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-5 Catalogue # 900-K15Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-5 Catalogue # 900-M15ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)66To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-6 Interleukin-6Synonyms: 26 kDa protein, IFN-β2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-6 200-06 E.coli 20.9 kDa 5µg 20µg 1mgMurine IL-6 216-16 E.coli 21.7 kDa 2µg 10µg 1mgRat IL-6 400-06 E.coli 21.7 kDa 2µg 10µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IL-6 AF-200-06 E.coli 20.9 kDa N/A 20µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-6 500-P26 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-6 500-P26bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human IL-6 500-P26G Goat I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-6 500-P26Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine IL-6 500-P56 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-6 500-P56bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IL-6 500-P73 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat IL-6 500-P73bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IL-6 500-P73G Goat E, WB, N 50µg 100µg 1mgBiot. anti-rat IL-6 500-P73Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IL-6 500-M06 Mouse I, E, WB, N 500µgKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIL-6 continued on next page...Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se67

www.peprotech.com2011-2012 CatalogueIL-6 continued...ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-6 Catalogue # 900-K16Murine IL-6 Catalogue # 900-K50Rat IL-6 Catalogue # 900-K86Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-6 Catalogue # 900-M16Rat IL-6 Catalogue # 900-M86ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)IHuman cervical squamous cell carcinoma (FFPE) stained with rabbitanti-human IL-6 at 0.5 µg/ml. Staining of tumor cells andextracellular expression.Tissues samples were provided by the Cooperative Human Tissue Network, which is funded by the NCI.Other investigators may have received specimens from the same subjects.Photographs provided by Patsy Ruegg for PeproTech.sIL-6Rα soluble Interleukin-6 receptor alphaSynonyms: B-cell stimulatory factor-2 receptor, CD126Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sIL-6Rα 200-06R HEK 293 cells 37.6 kDa 5µg 20µg 1mgAdditional sizes available please inquire68To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-7 Interleukin-7Synonyms: Lymphopoietin 1 (LP-1), pre-B cell factorRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-7 200-07 E.coli 17.4 kDa 2µg 10µg 1mgMurine IL-7 217-17 E.coli 15.0 kDa 2µg 10µg 1mgRat IL-7 400-07 E.coli 15.0 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-7 500-P27 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-7 500-P27bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-7 500-P57 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-7 500-P57bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IL-7 500-M07 Mouse E, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.IHuman IL-7 Catalogue # 900-K17ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)IL-8/CXCL8 Interleukin-8Synonyms: CXCL8, MDNCF, Neutrophil activating factor (NAF), NAP-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-8 (72 a.a.) 200-08M E.coli 8.4 kDa 5µg 25µg 1mgHuman IL-8 (77 a.a.) 200-08 E.coli 8.9 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-8 500-P28 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-8 500-P28bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIL-8 continued on next page...Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se69

www.peprotech.com2011-2012 CatalogueIL-8 continued...Normal human skin (FFPE) stained with rabbitanti-human IL-8 at 0.25 µg/ml. Photographby PeproTech.Monoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IL-8 500-M08 Mouse E, N, WB 500µgKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-8 Catalogue # 900-K18IMini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-8 Catalogue # 900-M18ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)IL-9 Interleukin-9Synonyms: p40 cytokine, T-cell growth factor p40Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-9 200-09 E.coli 14.0 kDa 2µg 10µg 1mgMurine IL-9 219-19 E.coli 14.3 kDa 2µg 10µg 1mgRat IL-9 400-18 E.coli 14.3 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-9 500-P29 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-9 500-P29bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-9 500-P59 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-9 500-P59bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-9 Catalogue # 900-K20ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)70To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-10 Interleukin-10Synonyms: B-TCGF, CSIF, TGIFRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-10 200-10 E.coli 18.6 kDa 2µg 10µg 1mgMurine IL-10 210-10 E.coli 18.7 kDa 2µg 10µg 1mgRat IL-10 400-19 E.coli 18.7 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-10 500-P20 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-10 500-P20bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-10 500-P60 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-10 500-P60bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IL-10 500-P139 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-rat IL-10 500-P139bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IL-10 500-M86 Mouse I, E, WB 500µgAnti-murine IL-10 500-M128 Rat E, WB, N 500µgAnti-rat IL-10 500-M130 Mouse E 500µgIELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-10 Catalogue # 900-K21Murine IL-10 Catalogue # 900-K53Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Murine IL-10 Catalogue # 900-M53ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se71

www.peprotech.com2011-2012 CatalogueIL-11 Interleukin-11Synonyms: AGIF (Adipogenesis inhibitory factor)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-11 200-11 E.coli 19.1 kDa 2µg 10µg 1mgMurine IL-11 220-11 E.coli 19.2 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-11 500-P01 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-11 500-P01bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IL-11 500-M11 Mouse E, WB, N 500µgIBioassay analysis of murine IL-11(proliferation) using T11 cells.72To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-12 Interleukin-12Synonyms: NKSF, CTL maturation factor, Cytotoxic lymphocyte maturation factor (CLMF), TSFRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-12 200-12 CHO cells 75.0 kDa 2µg 10µg 1mgMurine IL-12 210-12 CHO cells 75.0 kDa 2µg 10µg 1mgHuman IL-12 p40 200-12p40 CHO cells 40.0 kDa 2µg 10µg 1mgHuman IL-12 p80 200-12p80H Insect cells 80.0 kDa 2µg 10µg N/APolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-12 500-P154G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human IL-12 500-P154Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine IL-12 500-P155G Goat E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-12 500-P155Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IL-12 500-M12 Mouse E, WB 500µgAnti-murine IL-12 500-M59 Rat E, N 500µgIELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-12 Catalogue # 900-K96Murine IL-12 Catalogue # 900-K97Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-12 Catalogue # 900-M96Murine IL-12 Catalogue # 900-M97ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Standard curve using Human IL-12 ELISAdevelopment kit.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se73

www.peprotech.com2011-2012 CatalogueIL-13 Interleukin-13Synonyms: P600 (murine), NC30 (human)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-13 200-13 E.coli 12.5 kDa 2µg 10µg 1mgMurine IL-13 210-13 E.coli 12.3 kDa 2µg 10µg 1mgRat IL-13 400-16 E.coli 11.9 kDa 2µg 10µg 1mgRat IL-13 (113 a.a.) 400-16L E.coli 12.3 kDa 2µg 10µg 1mgHuman IL-13 Variant 200-13A E.coli 12.6 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-13 500-P13 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-13 500-P13bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-13 500-P178 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-13 500-P178bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-13 Catalogue # 900-K23Murine IL-13 Catalogue # 900-K207Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Murine IL-13 Catalogue # 900-M207ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)74To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-15 Interleukin-15Synonyms: IL-TRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-15 200-15 E.coli 12.9 kDa 2µg 10µg 1mgMurine IL-15 210-15 E.coli 13.3 kDa 2µg 10µg 1mgRat IL-15 400-24 E.coli 13.5 kDa 2µg 10µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IL-15 AF-200-15 E.coli 12.9 kDa N/A 10µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-15 500-P15 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-15 500-P15bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-15 500-P173 Rabbit E, WB, N 25µg 100µg 1mgBiot. anti-murine IL-15 500-P173bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human IL-15 500-M15 Mouse E, WB 500µgIL-16 Interleukin-16Synonyms: LCF (Lymphocyte Chemoattractant Factor)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-16 (121 a.a.) 200-16A E.coli 12.4 kDa 2µg 10µg 1mgHuman IL-16 (130 a.a.) 200-16 E.coli 13.5 kDa 2µg 10µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IL-16 AF-200-16A E.coli 12.4 kDa N/A 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-16 500-P06 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-16 500-P06bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se75

www.peprotech.com2011-2012 CatalogueIL-17A/IL-17 Interleukin-17ASynonyms: CTLA-8Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-17A/IL-17 200-17 E.coli 31.0 kDa 5µg 25µg 1mgMurine IL-17 210-17 E.coli 30.0 kDa 5µg 25µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IL-17 AF-200-17 E.coli 31.0 kDa N/A 25µg 1mgAdditional sizes available please inquireIPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-17A 500-P07 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-17A 500-P07bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human IL-17A 500-P07G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human IL-17A 500-P07Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine IL-17A 500-P265 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine IL-17A 500-P265bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-17A/IL-17 Catalogue # 900-K84Murine IL-17 Catalogue # 900-K392Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-17A Catalogue # 900-M84Murine IL-17A Catalogue # 900-M392ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)76To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-17B Interleukin-17BSynonyms: IL-20, NIRFRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-17B 200-28 E.coli 36.6 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-17B 500-P248 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human IL-17B 500-P248bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIL-17D Interleukin-17DSynonyms: IL-27Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-17D 200-27 E.coli 40.5 kDa 5µg 25µg 1mgAdditional sizes available please inquireIIL-17E Interleukin-17ESynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-17E 200-24 E.coli 33.8 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-17E 500-P89 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human IL-17E 500-P89bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-17E Catalogue # 900-K234Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-17E Catalogue # 900-M234ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se77

www.peprotech.com2011-2012 CatalogueIL-17F Interleukin-17FSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-17F 200-25 E.coli 30.1 kDa 5µg 25µg 1mgMurine IL-17F 210-17F E.coli 30.0 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-17F 500-P90 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-17F 500-P90bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-17F Catalogue # 900-K277IELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)IL-18BP Interleukin-18 Binding ProteinPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-murine IL-18BP 500-P153G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-murine IL-18BP 500-P153Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested78To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-19 Interleukin-19Synonyms: Melanoma differentiation associated like proteinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-19 200-19 E.coli 17.9 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-19 500-P189 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human IL-19 500-P189bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIL-20 Interleukin-20Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-20 200-20 E.coli 35.2 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-20 500-P190G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human IL-20 500-P190Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-20 Catalogue # 900-K224ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Standard curve using Human IL-20 ELISAdevelopment kit.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se79

www.peprotech.com2011-2012 CatalogueIL-21 Interleukin-21Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-21 200-21 E.coli 15.4 kDa 2µg 10µg 1mgMurine IL-21 210-21 E.coli 15.0 kDa 2µg 10µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman IL-21 AF-200-21 E.coli 15.4 kDa N/A 10µg 1mgAdditional sizes available please inquireIPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-21 500-P191 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-21 500-P191bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-21 500-P278 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine IL-21 500-P278bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-21 Catalogue # 900-K226ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)80To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-22 Interleukin-22Synonyms: IL-TIFRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-22 200-22 E.coli 33.6 kDa 2µg 10µg 1mgMurine IL-22 210-22 E.coli 33.4 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-22 500-P211 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human IL-22 500-P211bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IL-22 500-P223 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IL-22 500-P223bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-22 Catalogue # 900-K246IELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se81

www.peprotech.com2011-2012 CatalogueIL-23 Interleukin-23Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-23 200-23 Insect cells 53.5 kDa 2µg 10µg 1mgAdditional sizes available please inquireIL-31 Interleukin-31Synonyms: noneIRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-31 200-31 E.coli 15.8 kDa 2µg 10µg 1mgMurine IL-31 210-31 E.coli 15.9 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-31 500-P249 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human IL-31 500-P249bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-31 Catalogue # 900-K347Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-31 Catalogue # 900-M347ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)82To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comIL-33 Interleukin-33Synonyms: NF-HEVRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-33 200-33 E.coli 18.0 kDa 2µg 10µg 1mgMurine IL-33 210-33 E.coli 17.5 kDa 2μg 10μg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IL-33 500-P261 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human IL-33 500-P261bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IL-33 Catalogue # 900-K398Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human IL-33 Catalogue # 900-M398IELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)IL-34 Interleukin-34Synonyms: C16orf77Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IL-34 200-34 HEK 293 cells 52.5 kDa 2µg 10µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se83

www.peprotech.com2011-2012 CatalogueIP-10/CXCL10 γ-Interferon inducible protein-10Synonyms: CXCL10, crg-2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman IP-10 300-12 E.coli 8.5 kDa 5µg 25µg 1mgMurine IP-10 250-16 E.coli 8.7 kDa 5µg 25µg 1mgRat IP-10 400-33 E.coli 8.6 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human IP-10 500-P93 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human IP-10 500-P93bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine IP-10 500-P129 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine IP-10 500-P129bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat IP-10 500-P290 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat IP-10 500-P290bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedIELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human IP-10 Catalogue # 900-K39Murine IP-10 Catalogue # 900-K153Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Murine IP-10 Catalogue # 900-M153ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)84To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comI-TAC/CXCL11 Interferon inducible-T cell α chemokineSynonyms: CXCL11, B-R1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman I-TAC 300-46 E.coli 8.3 kDa 5µg 20µg 1mgMurine I-TAC 250-29 E.coli 9.0 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human I-TAC 500-P132 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human I-TAC 500-P132bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human I-TAC Catalogue # 900-K151ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)I-J-KHuman renal tumor with parenchyma (FFPE) stained withrabbit anti-human I-TAC at 0.25 µg/mL. Staining of vessellining cells and tumor cells.Tissue samples were provided by the Cooperative Human Tissue Network,which is funded by the National Cancer Institute. Other investigators may havereceived specimens from the same subjects.Photograph provided by Patsy Ruegg for PeproTech.JE/MCP-1/CCL2 (See MCP-1)Synonyms: CCL2, MCP-1KC/CXCL1 (See GROα)Synonyms: CXCL1, GROαYeast Kex-2Synonyms: Endoproteinase Lys/Arg-ArgRecombinant ProteinsProduct Catalogue # Source MW Size A Size BYeast Kex-2 450-45 Insect cells 59.9 kDa 50µg 250µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se85

www.peprotech.com2011-2012 CatalogueKGF/FGF-7 (See FGF-7)Synonyms: FGF-7, HBGFKLF4-TATSynonyms: Kruppel-like factor-4, EZF, GKLFRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman KLF4-TAT 110-08 HEK 293 cells 51.7 kDa 5µg 25µg 1mgAdditional sizes available please inquireKlothoSynonyms: αKlotho, KLAK-LRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Klotho 100-53 CHO cells 65.0-70.0 kDa 5μg 20μg 1mgAdditional sizes available please inquireLAG-1/CCL4L1 Lymphocyte activation gene-1Synonyms: CCL4L1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman LAG-1 300-58 E.coli 7.7 kDa 5µg 20µg 1mgAdditional sizes available please inquireLD78β/CCL3L1Synonyms: CCL3L1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman LD78β 300-56 E.coli 7.7 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human LD78β 500-P187G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human LD78β 500-P187Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested86To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comLEC/CCL16 Liver-expressed chemokineSynonyms: CCL16, NCC-4, HCC-4, LMC, LCC-1, MTN-1, IL-10-inducible chemokineRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman LEC 300-44 E.coli 11.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human LEC 500-P125G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human LEC 500-P125Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human LEC 500-M43 Mouse E, WB, N 500µgLeptinSynonyms: Obesity protein (OB)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Leptin 300-27 E.coli 16.0 kDa 200µg 1000µg 5mgMurine Leptin 450-31 E.coli 16.2 kDa 200µg 1000µg 5mgRat Leptin 400-21 E.coli 16.2 kDa 200µg 1000µg 5mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Leptin 500-P86 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human Leptin 500-P86bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine Leptin 500-P68 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-murine Leptin 500-P68bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat Leptin 500-P185G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-rat Leptin 500-P185Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedLMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human Leptin 500-M27 Mouse E, WB 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human Leptin Catalogue # 900-K90Murine Leptin Catalogue # 900-K76ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)See next page for IHC images of anti-murine Leptin...Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se87

www.peprotech.com2011-2012 CatalogueLeptin Continued…Murine tissue 4% PFA, paraffin-embedded sections of a Trichuris muris infected mouse stainedwith rabbit anti-murine Leptin at 0.20 µg/ml. Information and photographs are courtesy of KatherineGuild and David Artis, University of Pennsylvania.LIGHTLSynonyms: TNFSF14, HVEM-LRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman LIGHT 310-09B Insect cells 21.4 kDa 3µg 15µg N/AMurine LIGHT 315-12 E.coli 20.1 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human LIGHT 500-P179 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human LIGHT 500-P179bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedLin28-TATSynonyms: CSDD1, LIN28A, ZCCHC1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Lin28-TAT 110-06 E.coli 24.4 kDa 5µg 25µg 1mgAdditional sizes available please inquireLIX/CXCL5 (See ENA-78)Synonyms: CXCL5, ENA-78 (human)88To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comLungkineSynonyms: CXCL15Recombinant ProteinsProduct Catalogue # Source MW Size A Size BMurine Lungkine 250-37 E.coli 16.3 kDa 5µg 20µg 1mgAdditional sizes available please inquireLymphotactin/XCL1Synonyms: XCL1, SCM-1a, ATACRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Lymphotactin 300-20 E.coli 10.0 kDa 5µg 20µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Lymphotactin 500-P40 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human Lymphotactin 500-P40bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedL-MMaspinSynonyms: SerpinB5, Protease inhibitor 5Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Maspin 130-12 E.coli 42.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Maspin 500-P270 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human Maspin 500-P270bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedAnti-Human Maspin (Catalogue #500-P270)Human breast lobular carcinoma in situ (FFPE) stainedwith Rabbit Anti-Human Maspin at 7.8 ng/mL. Stainingof myoepithelial cells.Tissue samples were provided by the Cooperative Human Tissue Network,which is funded by the National Cancer Institute. Other investigators may havereceived specimens from the same subjects.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se89

PEPROTECH ®www.peprotech.comMCPs Monocyte chemotactic proteinsSynonyms: MCP-1: CCL2, JE (murine); MCP-2: CCL8, HC14; MCP-3: CCL7, MARC; MCP-4:CCL13, NCC-1; MCP-5: CCL12Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MCP-1 300-04 E.coli 8.6 kDa 5µg 20µg 1mgMurine JE/MCP-1 250-10 E.coli 13.8 kDa 2µg 10µg 1mgRat MCP-1 400-12 E.coli 14.0 kDa 2µg 10µg 1mgHuman MCP-2 300-15 E.coli 8.9 kDa 2µg 10µg 1mgMurine MCP-2 250-14 E.coli 8.5 kDa 5µg 20µg 1mgHuman MCP-3 300-17 E.coli 9.0 kDa 2µg 10µg 1mgMurine MCP-3 250-08 E.coli 8.5 kDa 2µg 10µg 1mgHuman MCP-4 300-24 E.coli 8.6 kDa 5µg 20µg 1mgMurine MCP-5 250-04 E.coli 9.3 kDa 5µg 20µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman MCP-1 AF-300-04 E.coli 8.6 kDa N/A 20µg 1mgAdditional sizes available please inquireMPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MCP-1 500-P34 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human MCP-1 500-P34bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine JE/MCP-1 500-P113 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine JE/MCP-1 500-P113bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedHuman breast invasive ductal carcinoma (FFPE) stained withrabbit anti-human MCP-1 at 2.5 µg/ml. Tear shaped mammaryduct with tumor cells and normal cells.Tissue samples were provided by the Cooperative Human Tissue Network, which is funded bythe NCI. Other investigators may have received specimens from the same subjects.Photographs by PeproTech.MCP continued on next page...Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se91

www.peprotech.com2011-2012 CatalogueMCP continued...MPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-rat MCP-1 500-P76 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat MCP-1 500-P76bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human MCP-2 500-P35 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human MCP-2 500-P35bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine MCP-2 500-P127 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine MCP-2 500-P127bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human MCP-3 500-P37 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human MCP-3 500-P37bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human MCP-3 500-P37G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human MCP-3 500-P37Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine MCP-3 500-P116G Goat E, WB, N 50µg 100µg 1mgBiot. anti-murine MCP-3 500-P116Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-human MCP-4 500-P04 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human MCP-4 500-P04bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human MCP-4 500-P04G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human MCP-4 500-P04Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine MCP-5 500-P61 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine MCP-5 500-P61bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human MCP-1 500-M71 Mouse I, E, WB 500µgAnti-human MCP-2 500-M69 Mouse E, WB, N 500µgAnti-human MCP-3 500-M73 Mouse E, WB, N 500µgAnti-human MCP-4 500-M70 Mouse E, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells..Human MCP-1 Catalogue # 900-K31Murine JE/MCP-1 Catalogue # 900-K126Rat MCP-1 Catalogue # 900-K59Murine MCP-3 Catalogue # 900-K123Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Murine JE/MCP-1 Catalogue # 900-M126ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)92To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comMDC/CCL22 Macrophage-derived chemokineSynonyms: CCL22, STCP-1, ABCD-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MDC (67 a.a.) 300-36 E.coli 8.0 kDa 5µg 20µg 1mgHuman MDC (69 a.a.) 300-36A E.coli 8.1 kDa 5µg 20µg 1mgMurine MDC 250-23 E.coli 7.8 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MDC 500-P107 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human MDC 500-P107bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine MDC 500-P176 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine MDC 500-P176bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human MDC 500-M41 Mouse E, WB 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.MMurine MDC Catalogue # 900-K197ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Standard curve using Murine MDC ELISAdevelopment kit.MEC/CCL28 Mucosae-associated epithelial chemokineSynonyms: CCL28, CCK-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MEC 300-57 E.coli 12.3 kDa 5µg 20µg 1mgMurine MEC 250-30 E.coli 12.6 kDa 5µg 20µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se93

www.peprotech.com2011-2012 CatalogueMIA Melanoma inhibitory activitySynonyms: CD-RAP (murine and rat)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MIA 130-01 E.coli 12.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MIA 500-P243 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human MIA 500-P243bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedHuman pancreas adenocarcinoma (FFPE)stained with rabbit anti-human MIA at 1.0 µg/ml.Tissue samples were provided by the Cooperative Human TissueNetwork, which is funded by the NCI. Other investigators may havereceived specimens from the same subjects.Photographs provided byPeproTech and Patsy Ruegg.MMIA-2 Melanoma inhibitory activity-2Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MIA-2 130-02 E.coli 11.5 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MIA-2 500-P255 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human MIA-2 500-P255bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human MIA-2 Catalogue # 900-K357ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Human pancreas infiltrating ductal adenocarcinoma tissue(FFPE)stained with rabbit anti-human MIA-2 at 5.0 µg/ml.Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by theNCI. Other investigators may have received specimens from the same subjects.Photograph by PeproTech.94To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comMidkineSynonyms: MK, NEGF-2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Midkine 450-16 E.coli 13.4 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Midkine 500-P171 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human Midkine 500-P171bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human Midkine Catalogue # 900-K190ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)MIG/CXCL9 Monokine induced by interferon-γMSynonyms: CXCL9Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MIG 300-26 E.coli 11.7 kDa 5µg 20µg 1mgMurine MIG 250-18 E.coli 12.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MIG 500-P50 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human MIG 500-P50bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human MIG 500-M42 Mouse E, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells..Human MIG Catalogue # 900-K87Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human MIG Catalogue # 900-M87ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se95

www.peprotech.com2011-2012 CatalogueMIP-1α and β Macrophage inflammatory proteins alpha and betaSynonyms: MIP-1α: CCL3, LD78αMIP-1β: CCL4, ACT-2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MIP-1α 300-08 E.coli 7.8 kDa 5µg 20µg 1mgMurine MIP-1α 250-09 E.coli 7.8 kDa 2µg 10µg 1mgRat MIP-1α 400-15 E.coli 7.8 kDa 5µg 20µg 1mgHuman MIP-1β 300-09 E.coli 7.6 kDa 2µg 10µg 1mgMurine MIP-1β 250-32 E.coli 7.8 kDa 2µg 10µg 1mgRat MIP-1β 400-09 E.coli 7.8 kDa 5µg 20µg 1mgAdditional sizes available please inquireMPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MIP-1α 500-P38 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human MIP-1α 500-P38bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human MIP-1α 500-P38G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human MIP-1α 500-P38Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine MIP-1α 500-P121 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine MIP-1α 500-P121bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat MIP-1α 500-P77 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat MIP-1α 500-P77bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human MIP-1β 500-P38B Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human MIP-1β 500-P38Bbt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine MIP-1β 500-P213 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine MIP-1β 500-P213bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human MIP-1α 500-M74 Mouse E, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human MIP-1α Catalogue # 900-K35Murine MIP-1α Catalogue # 900-K125Rat MIP-1α Catalogue # 900-K75Murine MIP-1b Catalogue # 900-K278Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human MIP-1a Catalogue # 900-M125Murine MIP-1b Catalogue # 900-M278ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)See next page for standard curve for Human MIP-1 alpha ELISA development kit...96To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comMIP-1a and b Continued…Standard curve using the Human MIP-1 alphaELISA development kit.MIP-1γ/CCL9/10 Macrophage inflammatory protein gammaSynonyms: CCL9/10, MRP-2, CCF18Recombinant ProteinsProduct Catalogue # Source MW Size A Size BMurine MIP-1γ 250-12 E.coli 11.6 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-murine MIP-1γ 500-P117 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-murine MIP-1γ 500-P117bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMMurine MIP-1γ induces chemotaxis ofpurified human monocytes.MIP-2/CXCL2 (See GROβ)Synonyms: CXCL2, GROβvMIP-2 viral Macrophage inflammatory protein-2Synonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BvMIP-2 350-03 E.coli 7.9 kDa 10µg 50µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se97

www.peprotech.com2011-2012 CatalogueMIP-3/CCL23 Macrophage inflammatory protein-3Synonyms: CCL23, MPIF-1, Ck-β8Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MIP-3 300-29 E.coli 11.3 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MIP-3 500-P124 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human MIP-3 500-P124bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMIP-3α/CCL20 Macrophage inflammatory protein-3 alphaMSynonyms: CCL20, LARC, Exodus-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MIP-3α 300-29A E.coli 8.0 kDa 5µg 20µg 1mgMurine MIP-3α 250-27 E.coli 7.9 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MIP-3α 500-P95A Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human MIP-3α 500-P95Abt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human MIP-3α 500-M28 Mouse E, WB 500µghCCR6/HEK293 Migration (Mean No./HPF±std)400350Binding Mediumh MIP-3a3002502001501005000 0.1 1 10 100 1000Concentration of Chemokines (ng/ml)Human MIP-3α induces chemotaxis of HEK293 cellstransfected with human CCR6.98To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comMIP-3β/CCL19 Macrophage inflammatory protein-3 betaSynonyms: CCL19, ELC, Exodus-3Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MIP-3β 300-29B E.coli 8.8 kDa 5µg 20µg 1mgMurine MIP-3β 250-27B E.coli 9.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MIP-3β 500-P95B Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human MIP-3β 500-P95Bbt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human MIP-3β 500-M29 Mouse E 500µgRepresentative Western Transfer (blot) analysis ofhuman MIP-3α using anti-human MIP-3β at 0.20µgml.kDa14860M4230221764MIP-4/CCL18 Macrophage inflammatory protein-4Synonyms: CCL18, PARC, DC-CK1, AMAC-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MIP-4 300-34 E.coli 7.8 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MIP-4 500-P108 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human MIP-4 500-P108bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se99

www.peprotech.com2011-2012 CatalogueMIP-5/CCL15 Macrophage inflammatory protein-5Synonyms: CCL15, HCC-2, Lkn-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MIP-5 300-43 E.coli 10.1 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human MIP-5 500-P123G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human MIP-5 500-P123Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMMP-1 Matrix metalloproteinase-1Synonyms: Fibroblast collagenase, Interstitial CollagenaseMRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MMP-1 420-01 E.coli 42.7 kDa 2µg 10µg 1mgAdditional sizes available please inquireMMP-2 Matrix metalloproteinase-2Synonyms: Gelatinase A, TBE-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MMP-2 420-02 E.coli 62.1 kDa 2µg 10µg 1mgAdditional sizes available please inquireMMP-3 Matrix metalloproteinase-3Synonyms: Stromelysin-1, SL-1, Transin-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman MMP-3 420-03 E.coli 42.8 kDa 2µg 10µg 1mgMycoplasma Arginine DeiminaseSynonyms: ADIRecombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BMycoplasma Arginine Deiminase 150-12 E.coli 46.4 kDa 5µg 20µg 1mgAdditional sizes available please inquire100To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comMyostatinSynonyms: GDF-8Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Myostatin 120-00 E.coli 25.0 kDa 2µg 10µg 1mgAdditional sizes available please inquireMyostatin PropeptideSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Myostatin Propeptide 120-12 E.coli 27.8 kDa 5µg 25µg 1mgAdditional sizes available please inquireNanogM-NSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Nanog 120-21 E.coli 34.7 kDa 5µg 20µg 1mgHuman Nanog-TAT 120-21B E.coli 36.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Nanog 500-P236 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human Nanog 500-P236bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedAnti-Human Nanog (Catalogue #500-P236)Human prostate malignant adenocarcinoma (FFPE)stained with Rabbit Anti-Human Nanog at 0.5 µg/mL.Staining of tumor cells.Tissue samples were provided by the Cooperative Human Tissue Network,which is funded by the National Cancer Institute. Other investigators may havereceived specimens from the same subjects.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se101

www.peprotech.com2011-2012 CatalogueNAP-2/CXCL7 Neutrophil activating protein-2Synonyms: CXCL7, PBP (parent molecule), CTAP-III (precursor)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman NAP-2 300-14 E.coli 7.6 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human NAP-2 500-P03 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human NAP-2 500-P03bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human NAP-2 500-P03G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human NAP-2 500-P03Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human NAP-2 500-M33 Mouse E 500µgNELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human NAP-2 Catalogue # 900-K40ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)NeuritinSynonyms: CPG15, NRN1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Neuritin 450-36D E.coli 19.4 kDa 5μg 20μg 1mgAdditional sizes available please inquireNeuropoietinSynonyms: NPO, NPRecombinant ProteinsProduct Catalogue # Source MW Size A Size BMurine Neuropoietin 250-25B E.coli 19.8 kDa 5µg 25µg 1mgAdditional sizes available please inquire102To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comNeuroserpinSynonyms: Serpin I1, Protease inhibitor 12Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Neuroserpin 130-14 E.coli 44.6 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Neuroserpin 500-P271 Rabbit E, WB, NA 50μg 100μg 1mgBiot. anti-human Neuroserpin 500-P271bt Rabbit E, WB, NA 25μg 50μg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedNeurturinSynonyms: NTN, NRTNRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Neurturin 450-11 E.coli 23.6 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Neurturin 500-P102 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human Neurturin 500-P102bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedNBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se103

www.peprotech.com2011-2012 Catalogueβ-NGF beta-Nerve growth factorSynonyms: NGF-betaRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman β-NGF 450-01 E.coli 27.0 kDa 5µg 20µg 1mgMurine β-NGF 450-34 E.coli 27.0 kDa 5µg 20µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman β-NGF AF-450-01 E.coli 27. 0 kDa N/A 20µg 1mgAdditional sizes available please inquireNPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human β-NGF 500-P85 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human β-NGF 500-P85bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human β-NGF 500-M85 Mouse E, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human β-NGF Catalogue # 900-K60ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)104To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comNNT-1/BCSF-3 Novel neurotrophin-1/B-cell stimulating factor-3Synonyms: Cardiotrophin-like cytokineRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman NNT-1/BCSF-3 450-18 E.coli 22.4 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human NNT-1/BCSF-3 500-P186 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human NNT-1/BCSF-3 500-P186bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedNOGGINSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman NOGGIN 120-10C HEK 293 cells 28.0-33.0 kDa 5µg 20µg 1mgMurine NOGGIN 250-38 E.coli 46.4 kDa 5µg 20µg 1mgAdditional sizes available please inquireNNOV Nephroblastoma overexpressed geneSynonyms: CCN3Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman NOV 120-26 E.coli 36.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human NOV 500-P257 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human NOV 500-P257bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human NOV Catalogue # 900-K338ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se105

www.peprotech.com2011-2012 CatalogueNP-1 Neutrophil Peptide-1Synonyms: alpha-defensin-1, HNP-1, CryptdinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman NP-1 300-42 E.coli 3.4 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS Size A Size B 1mgAnti-human NP-1 500-P126G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human NP-1 500-P126Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedNT-3 Neurotrophin-3Synonyms: Neurotrophic Factor, Nerve growth factor-2 (NGF-2), HGNFNRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman NT-3 450-03 E.coli 27.2 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human NT-3 500-P82 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human NT-3 500-P82bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human NT-3 500-P82G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human NT-3 500-P82Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedNT-4 Neurotrophin-4Synonyms: Neurotrophic 4/5 (NT-4/NT-5)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman NT-4 450-04 E.coli 28.1 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human NT-4 500-P83G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human NT-4 500-P83Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human NT-4 500-M24 Mouse E 500µg106To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comOct4-TATSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Oct4-TAT 110-04H HEK 293 cells 41.3 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Oct4-TAT 500-P289 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human Oct4-TAT 500-P289bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedOncostatin MSynonyms: OSMRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Oncostatin M (227 a.a.) 300-10 E.coli 26.0 kDa 2µg 10µg 1mgHuman Oncostatin M (209 a.a.) 300-10T E.coli 23.9 kDa 2µg 10µg 1mgRat Oncostatin M 400-36 E.coli 24.4 kDa 2µg 10µg 1mgAdditional sizes available please inquireOPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Oncostatin M 500-P30 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human Oncostatin M 500-P30bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedOsteopontinSynonyms: Bone sialoprotein 1, Secreted phosphoprotein 1, SPP-1, Nephropontin, UropontinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Osteopontin 120-35 HEK 293 cells 60.0-65.0 kDa 10µg 50µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se107

www.peprotech.com2011-2012 CatalogueOsteoprotegerinSynonyms: TNFRSF11B, OPG, OCIF (Osteoclastogenesis inhibitory factor),TR1 (TNF receptor like protein-1)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Osteoprotegerin (OPG) 450-14 E.coli 20.0 kDa 10µg 50µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human OPG 500-P149 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human OPG 500-P149bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedOHuman breast invasive ductal carcinoma (FFPE) stainedwith rabbit anti-human OPG at 5.0 µg/ml.Tissue samples were provided by the Cooperative Human Tissue Network, which is fundedby the NCI. Other investigators may have received specimens from the same subjects.Photograph by PeproTech.OTORSynonyms: Otoraplin, MIALRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman OTOR 130-03 E.coli 12.7 kDa 5µg 20µg 1mgAdditional sizes available please inquiresOX40 LigandSynonyms: sOX40L, Glycoprotein Gp34, CD252 antigen, TXGP1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sOX40L 310-28 Insect cells 15.5-25.0 kDa 2µg 10µg 1mgAdditional sizes available please inquire108To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comp16-INK4aSynonyms: Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, CDK4I,p16INK4A, Multiple tumor suppressor 1, MTS-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman p16-INK4a 110-02 E.coli 16.5 kDa 5μg 20μg 1mgHuman p16-INK4a-TAT 110-02T E.coli 18.0 kDa 5μg 25μg 1mgPAF-AHAdditional sizes available please inquireSynonyms: PAF acetylhydrolase, PAF 2-acylhydrolase, LDL-associated phopholipase A2, LDL-PLA (2),2-acetyl-alkylglycerophosphocholine esterase, 1-alkyl-2-acetylglycerophosphocholine esteraseRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PAF-AH 140-10 HEK 293 cells 47.0-55.0 kDa 5μg 20μg 1mgAdditional sizes available please inquirePAI-1 Plasminogen Activator Inhibitor-1Synonyms: Serpin E1PRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PAI-1 140-04 E.coli 42.7 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human PAI-1 500-P260 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human PAI-1 500-P260bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human PAI-1 Catalogue # 900-K383ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)PAI-2 Plasminogen Activator Inhibitor-2Synonyms: Serpin B2, Urokinase inhibitor, Monocyte Arg-serpinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PAI-2 140-06 E.coli 46.5 kDa 2µg 10µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se109

www.peprotech.com2011-2012 CataloguePDGF AA, AB, BB, CC Platelet-derived growth factorSynonyms: Glioma-derived growth factor (GDGF), Osteosarcoma-derived growth factor (ODGF)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PDGF-AA 100-13A E.coli 28.5 kDa 2µg 10µg 1mgMurine PDGF-AA 315-17 E.coli 28.8 kDa 2μg 10μg 1mgHuman PDGF-AB 100-00AB E.coli 25.5 kDa 2µg 10µg 1mgHuman PDGF-BB 100-14B E.coli 24.3 kDa 2µg 10µg 1mgMurine PDGF-BB 315-18 E.coli 24.6 kDa 2µg 10µg 1mgHuman PDGF-CC 100-00CC E.coli 25.0 kDa 5µg 20µg 1mgAdditional sizes available please inquirePAnimal-Free Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PDGF-BB AF-100-14B E.coli 24.3 kDa N/A 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human PDGF-AA 500-P46 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human PDGF-AA 500-P46bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human PDGF-BB 500-P47 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human PDGF-BB 500-P47bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human PDGF-BB Catalogue # 900-K04ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Normal human skin (FFPE) stainedwith rabbit anti-human PDGF-AAat 1.0 µg/ml. Photographs providedby Patsy Ruegg for PeproTech.Normal human skin (FFPE)stained with rabbit anti-humanPDGF-BB at 1.0 µg/ml.Granular staining of the sweatgland epithelial cells in the dermis.Photograph by PeproTech.110To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comPECAM-1Synonyms: Platelet endothelial cell adhesion molecule, CD31 antigen, EndoCamRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PECAM-1 150-06 HEK 293 cells 80.0-95.0 kDa 10µg 50µg 1mgAdditional sizes available please inquirePEDF Pigment Epithelium-Derived FactorSynonyms: Serpin F1, EPC-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PEDF 130-13 E.coli 44.5 kDa 5µg 20µg 1mgAdditional sizes available please inquirePersephinSynonyms: PSP, PSPNRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Persephin 450-12 E.coli 20.6 kDa 5µg 20µg 1mgMurine Persephin 450-35 E.coli 20.6 kDa 5µg 20µg 1mgAdditional sizes available please inquirePPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Persephin 500-P138 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human Persephin 500-P138bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedPF-4/CXCL4 Platelet factor-4Synonyms: CXCL4, Oncostatin A, IroplactRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PF-4 300-16 E.coli 7.8 kDa 5µg 20µg 1mgMurine PF-4 250-39 E.coli 8.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human PF-4 500-P05 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human PF-4 500-P05bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se111

www.peprotech.com2011-2012 CataloguePleiotrophinSynonyms: PTN, Heparin affin regulatory protein (HARP), Heparin-binding growth factor-8 (HBGF8),Osteoblast-specific factor (OSF-1)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Pleiotrophin 450-15 E.coli 15.4 kDa 5µg 20µg 1mgAdditional sizes available please inquirePlGF-1 Placenta growth factor-1Synonyms: PGFPRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PlGF-1 100-06 E.coli 29.7 kDa 5µg 25µg 1mgAdditional sizes available please inquireAnimal-Free Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PlGF-1 AF-100-06 E.coli 29.7 kDa N/A 25µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human PlGF-1 500-P226 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human PlGF-1 500-P226bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human PlGF-1 Catalogue # 900-K307ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Standard curve using Human PlGF-1ELISA development kit.112To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comPlGF-2 Placenta growth factor-2Synonyms: PGFLRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PlGF-2 100-56 E.coli 34.0 kDa 5μg 25μg 1mgAdditional sizes available please inquirePlGF-3 Placenta growth factor-3Synonyms: PGFLRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PlGF-3 100-57 E.coli 22.7 kDa 5μg 25μg 1mgAdditional sizes available please inquirePProkineticin-2Synonyms: PK2, Protein Bv8 homologRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Prokineticin-2 100-46 E.coli 8.8 kDa 5µg 20µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se113

www.peprotech.com2011-2012 CatalogueProlactinSynonyms: Mammotropin, luterotropic hormone, lutetropinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Prolactin 100-07 E.coli 23.0 kDa 10µg 50µg 1mgMurine Prolactin 315-16 E.coli 22.5 kDa 10µg 50µg 1mgRat Prolactin 400-27 E.coli 22.6 kDa 10µg 50µg 1mgAdditional sizes available please inquirePTHrP Parathyroid hormone-related proteinSynonyms: nonePRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman PTHrP 100-09 E.coli 9.8 kDa 10µg 50µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human PTHrP 500-P276 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human PTHrP 500-P276bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested114To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comsRANKL soluble Receptor activator of NFkB ligandSynonyms: TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL (Osteoprotegerinligand), ODF (Osteoclast differentiation factor)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sRANKL 310-01 E.coli 20.0 kDa 2µg 10µg 1mgMurine sRANKL 315-11 E.coli 19.4 kDa 2µg 10µg 1mgRat sRANKL 400-30 E.coli 19.6 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human sRANKL 500-P133 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human sRANKL 500-P133bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human sRANKL 500-P133G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human sRANKL 500-P133Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine sRANKL 500-P63 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine sRANKL 500-P63bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human sRANKL 500-M46 Mouse E 500µgRELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human sRANKL Catalogue # 900-K142Murine sRANKL Catalogue # 900-K233ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Human metastatic carcinoma of lymph node (FFPE) from breast tissue stained withrabbit anti-human sRANKL at 0.25 µg/ml. Staining of membranous cells located in the germinal center.Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the NCI. Other investigators may have receivedspecimens from the same subjects.Photographs by PeproTech.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se115

www.peprotech.com2011-2012 CataloguesRANK Receptor soluble RANK ReceptorSynonyms: TNFRSF11A, TRANCE Receptor (TNF-related activation-induced cytokine receptor), ODAR(Osteoclast differentiation and activation receptor), ODFR (Osteoclast differentiation factor receptor)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sRANK Receptor 310-08 E.coli 19.3 kDa 20µg 100µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human sRANK Receptor 500-P144 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human sRANK Receptor 500-P144bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedRHuman breast invasive ductal carcinoma (FFPE) stained withrabbit anti-human sRANK receptor at 1.0 µg/ml.Tissue samples were provided by the Cooperative Human Tissue Network, which is fundedby the NCI. Other investigators may have received specimens from the same subjects.Photograph by PeproTech.116To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

www.peprotech.com2011-2012 CatalogueRelaxin-2Synonyms: H2 relaxin, RLN2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Relaxin-2 130-15 E.coli 6.0 kDa 5µg 25µg 1mgAdditional sizes available please inquireRelaxin-3Synonyms: H3 relaxin, Insulin-like peptide-7, INSL7Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Relaxin-3 130-10 E.coli 5.5 kDa 5µg 25µg 1mgAdditional sizes available please inquireRRELMα Resistin-like alphaSynonyms: Cysteine-rich secreted protein FIZZ1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BMurine RELMα 450-26 E.coli 9.5 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-murine RELMα 500-P214 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-murine RELMα 500-P214bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMurine cecum tissue (4% PFA, paraffin-embedded sectionswith positive cells infiltrating the Lamina Propia of a Trichurismuris infected mouse) stained with rabbit anti-murine RELMαat 4.0 ng/ml. Information and photograph are courtesy ofKatherine Guild and David Artis, University of Pennsylvania.118To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comRELMβ Resistin-like betaSynonyms: Cysteine-rich secreted protein FIZZ2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman RELMβ 450-22 E.coli 19.0 kDa 5µg 25µg 1mgMurine RELMβ 450-26B E.coli 18.0 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human RELMβ 500-P217 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human RELMβ 500-P217bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine RELMβ 500-P215 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-murine RELMβ 500-P215bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMurine cecum tissue (4% PFA, paraffin-embedded sectionswith positive goblet cells of a Trichuris muris infected mouse)stained with rabbit anti-murine RELMβ at 0.33 ng/ml. Informationand photograph are courtesy of Katherine Guild and David Artis,University of Pennsylvania.RRELMγ Resistin-like gammaSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BMurine RELMγ 450-26G E.coli 18.6 kDa 5µg 25µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se119

www.peprotech.com2011-2012 CatalogueResistinSynonyms: FIZZ3, Adipose tissue-specific secretory factor, ADSFRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Resistin 450-19 E.coli 19.5 kDa 5µg 25µg 1mgMurine Resistin 450-28 E.coli 20.2 kDa 5µg 25µg 1mgRat Resistin 400-35 E.coli 20.2 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Resistin 500-P183G Goat I, E, WB, NA 50µg 100µg 1mgBiot. anti-human Resistin 500-P183Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine Resistin 500-P182G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-murine Resistin 500-P182Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedRMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human Resistin 500-M91 Mouse I, E, WB 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human Resistin Catalogue # 900-K235ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Standard curve using human ResistinELISA development kit.120To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comSCF Stem cell factorSynonyms: c-Kit Ligand, Mast cell growth factor (MGF), Steel factor (SF)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman SCF 300-07 E.coli 18.4 kDa 2µg 10µg 1mgMurine SCF 250-03 E.coli 18.3 kDa 2µg 10µg 1mgRat SCF 400-22 E.coli 18.4 kDa 2µg 10µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman SCF AF-300-07 E.coli 18.4 kDa N/A 10µg 1mgMurine SCF AF-250-03 E.coli 18.3 kDa N/A 10µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human SCF 500-P48 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human SCF 500-P48bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human SCF 500-P48G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human SCF 500-P48Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine SCF 500-P71 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine SCF 500-P71bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat SCF 500-P202 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat SCF 500-P202bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedSMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human SCF 500-M44 Mouse E, WB 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human SCF Catalogue # 900-K34Murine SCF Catalogue # 900-K78Rat SCF Catalogue # 900-K258ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Bioassay analysis of human SCF (proliferation)using human TF-1 cells.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se121

www.peprotech.com2011-2012 CatalogueSCGF-α and β Stem cell growth factor alpha and betaSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman SCGF-α 100-22A E.coli 33.9 kDa 2µg 10µg 1mgHuman SCGF-β 100-22B E.coli 25.0 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human SCGF-β 500-P99G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human SCGF-β 500-P99Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedSDF-1α and β/CXCL12 Stromal-cell derived factor-1 alpha and betaSynonyms: CXCL12, PBSFSRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman SDF-1α 300-28A E.coli 8.0 kDa 2µg 10µg 1mgMurine SDF-1α 250-20A E.coli 7.9 kDa 2µg 10µg 1mgRat SDF-1α 400-32A E.coli 7.9 kDa 2μg 10μg 1mgHuman SDF-1β 300-28B E.coli 8.5 kDa 2µg 10µg 1mgMurine SDF-1β 250-20B E.coli 8.5 kDa 2µg 10µg 1mgRat SDF-1β 400-32B E.coli 8.4 kDa 2μg 10μg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquiProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human SDF-1α 500-P87A Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human SDF-1α 500-P87Abt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine SDF-1α 500-P164G Goat E, WB, N 50µg 100µg 1mgBiot. anti-murine SDF-1α 500-P164Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-human SDF-1β 500-P87BG Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human SDF-1β 500-P87BGbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedHuman colorectal adenocarcinoma (FFPE) stainedwith rabbit anti-human SDF-1α at 0.5 µg/mL.Tissue samples were provided by the Cooperative Human TissueNetwork, which is funded by the National Cancer Institute. Other investigatorsmay have received specimens from the same subjects.Photograph provided by Patsy Ruegg for PeproTech.122To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

www.peprotech.com2011-2012 CatalogueSPARC/OsteonectinSynonyms: Secreted protein acidic and rich in cysteine, BM-40, ONRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman SPARC/Osteonectin 120-36 CHO cells 32.0 kDa 10μg 50μg 1mgAdditional sizes available please inquireTACI Transmembrane activator and CAML interactorSynonyms: TNFRSF13BS-TRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TACI 310-17 E.coli 17.8 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TACI 500-P166G Goat E, WB, NA 50µg 100µg 1mgBiot. anti-human TACI 500-P166Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human TACI Catalogue # 900-K216ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)124To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comTAFA-2Synonyms: Chemokine-like protein TAFA-2, FAM19A2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TAFA-2 300-63 E.coli 11.2 kDa 5µg 20µg 1mgAdditional sizes available please inquireTARC/CCL17 Thymus activation regulation chemokineSynonyms: CCL17, ABCD-2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TARC 300-30 E.coli 8.0 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TARC 500-P97 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human TARC 500-P97bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedTTECK/CCL25 Thymus-expressed chemokineSynonyms: CCL25Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TECK 300-45 E.coli 14.2 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TECK 500-P134 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human TECK 500-P134bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human TECK 500-M48 Mouse E, WB, N 500µgBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se125

www.peprotech.com2011-2012 CatalogueTFF TreFoil FactorsSynonyms: TFF-1: pS2 protein, HP1.A, Breast cancer estrogen-inducible protein, PNR2TFF-2: Spasmolytic polypeptide (SP), SpasmolysinTFF-3: Intestinal trefoil, ITF, TFIRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TFF1 300-60 E.coli 8.2 kDa 5µg 20µg 1mgHuman TFF2 300-59 E.coli 12.0 kDa 5µg 20µg 1mgHuman TFF3 300-61 E.coli 13.2 kDa 5µg 20µg 1mgAdditional sizes available please inquireTGF-α Transforming growth factor alphaSynonyms: Sarcoma growth factor, TGF-type I, ETGFTRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TGF-α 100-16A E.coli 5.5 kDa 20µg 100µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TGF-α 500-P16 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human TGF-α 500-P16bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedHuman breast invasive ductal carcinoma(FFPE) stained with rabbit anti-humanTGF-α at 0.5 µg/ml.Tissue samples were provided by the Cooperative Human TissueNetwork, which is funded by the NCI. Other investigators mayhave received specimens from the same subjects.Photographs by PeproTech.126To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comTGF-β1,2 and 3 Transforming growth factor beta1, 2 and 3Synonyms: TGF-β1: Differentiation inhibiting factor, cartilage-inducing factorTGF-β2: Glioblastoma-derived T-cell suppressor factor, BSC-1, Cetermin, PolyerginTGF-β3: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TGF-β1 100-21 HEK 293 cells 25.0 kDa 1µg 5µg 1mgHuman TGF-β1 100-21C CHO cells 25.0 kDa 1µg 5µg 1mgHuman TGF-β2 100-35 Insect cells 25.0 kDa 1µg 5µg 1mgHuman TGF-β2 100-35B HEK 293 cells 25.0 kDa 1µg 5µg 1mgHuman TGF-β3 100-36E E.coli 25.0 kDa 2µg 10µg 1mgAdditional sizes available please inquireMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human TGF-β1 500-M66 Mouse I, E, WB 500µgKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedThrombomodulinSynonyms: CD141, BDCA-3, THBD, TMRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Thrombomodulin 100-58 HEK 293 cells 51.4 kDa 2µg 10µg 1mgAdditional sizes available please inquireTTIGARSynonyms: TP53-induced glycolysis and apoptosis regulatorRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TIGAR 150-14 E.coli 29.9 kDa 5μg 25μg 1mgHuman TIGAR-TAT 150-14T E.coli 31.6 kDa 5μg 25μg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se127

www.peprotech.com2011-2012 CatalogueTIMP-1 Tissue inhibitor of metalloproteinases 1Synonyms: Fibroblast collagenase inhibitor; Erythroid-potentiating activityRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TIMP-1 410-01 E.coli 20.6 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TIMP-1 500-P280 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human TIMP-1 500-P280bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human TIMP-1 Catalogue # 900-K438TMini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human TIMP-1 Catalogue # 900-M438ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Anti-Human TIMP-1 (Catalogue #500-P280) *Human breast invasive ductal carcinoma (FFPE) stainedwith Rabbit Anti-Human TIMP-1 at 0.100 µg/mL.Staining of tumor cells.Tissue samples were provided by the Cooperative Human Tissue Network, whichis funded by the National Cancer Institute. Other investigators may have receivedspecimens from the same subjects.TIMP-2 Tissue inhibitor of metalloproteinases 2Synonyms: CSC-21KRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TIMP-2 410-02 E.coli 21.8 kDa 2μg 10μg 1mgAdditional sizes available please inquire128To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comTL-1ASynonyms: TNFSF15, VEGIRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TL-1A 310-23 E.coli 22.0 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TL-1A 500-P240 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human TL-1A 500-P240bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human TL-1A Catalogue # 900-K290ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)TTLR-3 Toll-like receptor 3Synonyms: CD283 antigenRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TLR-3 160-01 HEK 293 cells 77.0 kDa 5μg 25μg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se129

www.peprotech.com2011-2012 CatalogueTNF-α Tumor necrosis factor alphaSynonyms: TNFSF1A, TNFSF2, cachectin, differentiation-inducing factor (DIF), necrosin, cytotoxinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TNF-α 300-01A E.coli 17.4 kDa 10µg 50µg 1mgMurine TNF-α 315-01A E.coli 17.3 kDa 5µg 20µg 1mgRat TNF-α 400-14 E.coli 17.3 kDa 5µg 20µg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman TNF-α AF-300-01A E.coli 17.4 kDa N/A 50µg 1mgAdditional sizes available please inquireTPolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TNF-α 500-P31A Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human TNF-α 500-P31Abt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human TNF-α 500-P31AG Goat I, E, WB, N 50µg 100µg 1mgBiot. anti-human TNF-α 500-P31AGbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine TNF-α 500-P64 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine TNF-α 500-P64bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-murine TNF-α 500-P64G Goat E, WB, N 50µg 100µg 1mgBiot. anti-murine TNF-α 500-P64Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-rat TNF-α 500-P72 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-rat TNF-α 500-P72bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human TNF-α 500-M26 Mouse I, E, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human TNF-α Catalogue # 900-K25Murine TNF-α Catalogue # 900-K54Rat TNF-α Catalogue # 900-K73Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human TNF-α Catalogue # 900-M25Murine TNF-α Catalogue # 900-M54ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)See next page for IHC images of anti-human TNF-α130To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comTNF-α Continued…Human breast invasive ductal carcinoma (FFPE) stained withrabbit anti-human TNF-α at 0.5 µg/ml.Tissue samples were provided by the Cooperative Human Tissue Network, which is fundedby the NCI. Other investigators may have received specimens from the same subjects.Photograph by PeproTech.TNF-β Tumor necrosis factor betaSynonyms: TNFSF1, TNFSF1B, Lymphotoxin alpha (LT-α)Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TNF-β 300-01B E.coli 18.6 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TNF-β 500-P31B Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human TNF-β 500-P31Bbt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedTsTNF Receptor Type I soluble TNF Receptor Type ISynonyms: TNFRSF1A, TNFAR, TNF-R55, TNFR60, p55, CD120aRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sTNF Receptor Type I 310-07 E.coli 18.3 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human sTNF Receptor Type I 500-P143 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human sTNF Receptor Type I 500-P143bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedBioassay analysis of human sTNF Receptor Type I.Determined by its inhibitory effect for the TNF-αmediated cytotoxicity in murine L929 cells.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se131

www.peprotech.com2011-2012 CataloguesTNF Receptor Type II soluble TNF Receptor Type IISynonyms: TNFRSF1B, p75, TNFR75, CD120b, TNFR80, p80, TNFR2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sTNF Receptor Type II 310-12 E.coli 18.9 kDa 5µg 20µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human sTNF Receptor Type II 500-P168 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-human sTNF Receptor Type II 500-P168bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedTPO ThrombopoietinSynonyms: Megakaryocyte colony-stimulating factor, c-MPL ligand, MGDFTRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TPO 300-18 E.coli 18.6 kDa 2µg 10µg 1mgMurine TPO 315-14 E.coli 18.7 kDa 2µg 10µg 1mgRat TPO 400-34 E.coli 18.7 kDa 2μg 10μg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman TPO AF-300-18 E.coli 18.6 kDa N/A 10µg 1mgRat TPO AF-400-34 E.coli 18.7 kDa N/A 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TPO 500-P49G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human TPO 500-P49Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human TPO Catalogue # 900-K44ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Standard curve using human TPOELISA development kit.132To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comsTRAIL/Apo2L soluble TNF-related apoptosis inducing ligandSynonyms: TNFSF10, Apo2 ligand, TL2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sTRAIL/Apo2L 310-04 E.coli 19.6 kDa 10µg 50µg 1mgMurine sTRAIL 315-19 E.coli 20.0 kDa 10μg 50μg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human sTRAIL/Apo2L 500-P135 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human sTRAIL/Apo2L 500-P135bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human sTRAIL/Apo2L 500-M49 Mouse E 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human sTRAIL/Apo2L Catalogue # 900-K141TELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)Human metastatic carcinoma of lymph node from breasttissue (FFPE) stained with rabbit anti-human sTRAIL/Apo2L at 0.25 µg/ml.Tissue samples were provided by the Cooperative Human Tissue Network,which is funded by the NCI. Other investigators may have received specimensfrom the same subjects.Photograph by PeproTech.sTRAIL Receptor-1/DR4 soluble TRAIL Receptor-1Synonyms: TNFRSF10A, DR4, Apo2Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sTRAIL Receptor-1/DR4 310-18 E.coli 22.7 kDa 10µg 50µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se133

www.peprotech.com2011-2012 CataloguesTRAIL Receptor-2/DR5 soluble TRAIL Receptor-2Synonyms: TNFRSF10B, DR5, KILLER, TRICK2A, TRICK2BRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman sTRAIL Receptor-2/DR5 310-19 E.coli 14.9 kDa 10µg 50µg 1mgAdditional sizes available please inquireTSG Twisted Gastrulation ProteinSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TSG 120-09 E.coli 22.2 kDa 10µg 50µg 1mgAdditional sizes available please inquireTTSLP Thymic stromal lymphopoietin proteinSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TSLP 300-62 E.coli 15.1kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TSLP 500-P258 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human TSLP 500-P258bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human TSLP Catalogue # 900-K334ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)134To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comTWEAK TNF-related weak inducer of apoptosisSynonyms: TNFSF12, DR3LG, Apo3 LigandRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TWEAK 310-06 E.coli 17.0 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human TWEAK 500-P137G Goat E, WB, N 50µg 100µg 1mgBiot. anti-human TWEAK 500-P137Gbt Goat E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human TWEAK Catalogue # 900-K149ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)TTWEAK Receptor TNF-related weak inducer of apoptosis receptorSynonyms: TNFRSF12A, FGF-inducible 14Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman TWEAK Receptor 310-21 E.coli 5.6 kDa 5µg 25µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se135

www.peprotech.com2011-2012 CatalogueVaspinSynonyms: Visceral adipose tissue-derived serpinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Vaspin 130-11 E.coli 45.2 kDa 5µg 25µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Vaspin 500-P256 Rabbit E, WB, NA 50µg 100µg 1mgBiot. anti-human Vaspin 500-P256bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedkDa148Representative Western Transfer (blot)analysis of human Vaspin using anti-humanVaspin at 0.20 µg/ml.6042V30221764VCAM-1Synonyms: CD106, INCAM-100, MGC108734, MGC99561, VCAM, VCAM1, VCAM1B, VECAM1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman VCAM-1 150-04 HEK 293 cells 74.1 kDa 10µg 50µg 1mgAdditional sizes available please inquire136To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comVEGF Vascular endothelial cell growth factorSynonyms: VEGF-A, VPF, VAS, folliculostellate cell-derived growth factor, glioma-derived endothelialcell mitogenRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman VEGF 121100-20A E.coli 28.4 kDa 2µg 10µg 1mgHuman VEGF 165100-20 E.coli 38.2 kDa 2µg 10µg 1mgMurine VEGF 165450-32 E.coli 39.0 kDa 2µg 10µg 1mgRat VEGF 165400-31 E.coli 38.5 kDa 2μg 10μg 1mgAnimal-Free Recombinant ProteinsAdditional sizes available please inquireProduct Catalogue # Source MW Size A Size BHuman VEGF AF-100-20 E.coli 38.2 kDa N/A 10µg 1mgAdditional sizes available please inquirePolyclonal Antibodies - Antigen Affinity PurifiedProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human VEGF 500-P10 Rabbit I, E, WB, N 50µg 100µg 1mgBiot. anti-human VEGF 500-P10bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-human VEGF 500-P10G Goat I, E, WB, N 50µg 100µg 1mgBiot. anti-human VEGF 500-P10Gbt Goat E, WB, NA 25µg 50µg 1mgAnti-murine VEGF 500-P131 Rabbit E, WB, N 50µg 100µg 1mgBiot. anti-murine VEGF 500-P131bt Rabbit E, WB, NA 25µg 50µg 1mgAnti-rat VEGF 500-P275 Rabbit E, WB, N 50μg 100μg 1mgBiot. anti-rat VEGF 500-P275bt Rabbit E, WB, NA 25μg 50μg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedVMonoclonal AntibodiesProduct Catalogue # Source APPLICATIONS SizeAnti-human VEGF 500-M88 Mouse E, WB, N 500µgELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 1000 ELISA plate wells.Human VEGF Catalogue # 900-K10Murine VEGF Catalogue # 900-K99Rat VEGF Catalogue # 900-K436Mini ELISA Development KitEach kit contains: capture antibody, detection antibody, calibrated standard, and Avidin-HRP Conjugate, sufficient to assay the target cytokine in at least 200 ELISA plate wells.Human VEGF Catalogue # 900-M10Rat VEGF Catalogue # 900-M436ELISA Buffer Kit.ELISA Buffer Kit Catalogue # 900-K00ELISA Buffer Kit and EDK Combination (For combined package price, please contact your local office.)See next page for IHC images of anti-human VEGFBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se137

PEPROTECH ®www.peprotech.comVEGF-D Vascular endothelial cell growth factor-DSynonyms: c-fos-induced growth factor, FIGFRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman VEGF-D 100-20D HEK 293 cells 20.0-26.0 kDa 2μg 10μg 1mgAdditional sizes available please inquireVisfatinSynonyms: PBEFRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Visfatin 130-09 E.coli 52.2 kDa 5µg 25µg 1mgAdditional sizes available please inquireVitronectinV-WSynonyms: VTN, Serum-spreading factor, V75Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Vitronectin 140-09 HEK 293 cells 52.2 kDa 50µg 250µg 1mgAdditional sizes available please inquireWISP-1 Wnt-1 inducible signaling pathway protein-1Synonyms: CCN4, Wnt-1-induced secreted proteinRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman WISP-1 120-18 E.coli 38.1 kDa 5µg 20µg 1mgAdditional sizes available please inquireWISP-3 Wnt-1 inducible signaling pathway protein-3Synonyms: CCN6Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman WISP-3 120-20 E.coli 36.8 kDa 5µg 20µg 1mgAdditional sizes available please inquireBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se139

www.peprotech.com2011-2012 CatalogueWnt-1Synonyms: INT-1Recombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Wnt-1 120-17 E.coli 38.1 kDa 2µg 10µg 1mgPolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Wnt-1 500-P250 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human Wnt-1 500-P250bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not testedWHuman breast invasive ductal carcinoma (FFPE) stained with rabbit anti-humanWnt-1 at 62.5 ng/mL. Staining of ductal carcinoma cells.Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the NationalCancer Institute. Other investigators may have received specimens from the same subjects.Photograph provided by Patsy Ruegg for PeproTechWnt-3aSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Wnt-3a 120-27C HEK 293 cells 38.1 kDa 2µg 10µg 1mgMurine Wnt-3a 315-20 cell culture 38.8-41.0 kDa 2µg 10µg N/APolyclonal Antibodies - Antigen Affinity PurifiedAdditional sizes available please inquireProduct Catalogue # Source APPLICATIONS SIZE A SIZE BAnti-human Wnt-3a 500-P251 Rabbit I, E, WB, NA 50µg 100µg 1mgBiot. anti-human Wnt-3a 500-P251bt Rabbit E, WB, NA 25µg 50µg 1mgBiot. = BiotinylatedKey: I = IHC, E = ELISA, WB = Western blot, N = Neutralization, NA = Neutralization not tested140To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comWnt-7aSynonyms: noneRecombinant ProteinsProduct Catalogue # Source MW Size A Size BHuman Wnt-7a 120-31 HeLa cells 35.5 kDa 3µg 15µg N/AAdditional sizes available please inquireWControl AntibodiesProduct Catalogue # SizeControl Goat Immunoglobulin 500-G00 1mgControl Mouse Immunoglobulin 500-M00 250µgControl Rabbit Immunoglobulin 500-P00 500µgBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se141

www.peprotech.com2011-2012 CatalogueChemokine NomenclatureINFORMATION & TECHNICAL SUPPORTKEYTm = memory T cellsB = B-cellsactT = activated T cellBs = BasophilT = resting T cellEo = EosinophilNK = Natural Killer cellsMo = monocytePMN = neutrophilsmDC = mature dendritic cellsiDC = immature DC142To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comFGF FamilyFibroblast Growth Factor familyFibroblast Growth FactorsMW-Secretedform (murine)EstimatedpITarget Cells(Partial List)ReceptorsFunctions(Partial list)FGF1 (Acidic FGF) 15.8 7.99 mesenchymal, all FGF Angiogenic in vivo,Heparin-binding growth factor-1 (HBGF-1) (15.7kDa) (7.88) neuroectodermal receptors Mitogenic in vitro,Beta-endothelial cell growth factor endothelial cells wound healingECGF-beta (endothelial cell growth factor-beta)FGF2 (Basic FGF) 17.2 10.03 mesenchymal, 1b,1c, Vasculogenesis, woundHeparin-binding growth factor-2 (HBGF-2) (17.1kDa) (9.59) neuroectodermal 2c, 3c,4 healing, angiogensis,Prostatropin endothelial cells hematopoiesis,neuron survivalFGF3 24.9 10.98 epithelial cells 2b Mesoderm induction,int-2 (25.2kDa) (10.84) that express FGF angiogenesis, inner earreceptor 2bdevelopmentFGF4 19.7 10.22 cells that express 1c, Angiogenesis,Heparin secretory transforming protein (HST-1) (18.9kDa) (10.24) FGF receptors 2c, 3c,4 vertabrate limbTransforming protein KS3development andHeparin-binding growth factor-4 (HBGF-4)development ofstomach cancerFGF5 27.5 11.1 cells that express 1c,2c Hair growth andHeparin-binding growth factor-5 (HBGF-5) (27.1kDa) (10.50) FGF receptors developmentSmag-82FGF6 18.7 10.09 cells that express 1c,2c,4 Skeletal muscleHeparin-binding growth factor-6 (HBGF-6) (18.9kDa) (9.80) FGF receptors developmentHST-2FGF7/KGF (Kerotinocyte Growth Factor) 18.9 9.55 keratinocytes and 2b Keratinocyte growthHeparin-binding growth factor-7 (HBGF-7) (18.8kDa) (9.22) epithelial cells factor, kidney and lungthat express FGFdevelopment,receptor 2bangiogenesis andwound healingFGF8 22.4 10.95 mammary 2c,3c,4 Limb, centralAndrogen-induced growth factor (AIGF) (28.0kDa) (10.46) carcinoma cells (possibly 1c) nervous system,Heparin-binding growth factor-8 (HBGF-8) and other cells cardiac outflow tractthat expressdevelopmentFGF receptorsFGF9 23.4 7.44 glial cells, 1c,2c, Glia-activating factor,Glia activating factor (GAF) (23.3kDa) (7.44) astrocytes cells 3b,3c,4 motoneuron survival,Heparin-binding growth factor-9 (HBGF-9) and other cells lung and testesthat express FGFdevelopmentreceptorsINFORMATION & TECHNICAL SUPPORTFGF10 19.3 10.49 epithelial cells 2b Wound healing,FGFA (19.5kDa) (9.76) that express FGF multi-organKeratinocyte growth factor 2 receptor 2b development includinglimb and lungFGF11 25.0 9.92 none Appears to be involvedFGFB (25.1kDa) (10.06) in nervous systemFibroblast Growth factor homologousdevelopmentfactor-3 (FHF-3)and functionFGF12 27.4kDa 9.98 none Appears to be involvedFGFCin nervous systemFibroblast Growth factor homologousdevelopmentfactor-1 (FHF-1)and functionContinued…Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se143

www.peprotech.com2011-2012 CatalogueFGF Family ContinuedFibroblast Growth FactorsMW-Secretedform (murine)EstimatedpITarget Cells(Partial List)ReceptorsFunctions(Partial list)FGF13 27.5 9.92 none Appears to be involvedFGFD (27.6 kDa) (9.92) in nervous systemFibroblast Growth factor homologousdevelopmentfactor-2 (FHF-2)and functionINFORMATION & TECHNICAL SUPPORTFGF14 27.7 10.11 none Regulates centralFGFE (27.7 kDa) (10.11) nervous systemFibroblast Growth factor homologousdevelopmentfactor-4 (FHF-4)and functionFGF15 (22.5 kDa) (5.76) cells that express unknown at Regulator of cellFGFF, identified in mouse not human FGF Receptor 4 time of division and patterningprinting in specific regions ofembroyonic brain,spinal cord andsensory organsFGF16 23.7 kDa 9.52 cells that express 2c, 3c Central Nervous SystemFGFG FGF receptors developmentFGF17 22.7 11.22 cells that express 1c, 2c, Signals induction andFGFH (22.5kDa) (10.71) FGF receptors 3c, 4 patterning of embryonicbrainFGF18 21.2 10.67 cells that express 1c, 2c, An essential regulatorzFGF5 (20.9kDa) (10.09) FGF receptors 3c, 4 of long bone andFGFIcalvarial developmentFGF19 21.8 kDa 6.32 cells that express 4 Expressed during brainFGFJ, identified in human not mouse FGF Receptor 4 development andduring embryogenesisFGF20 23.2 kDa 9.22 epithelial and 1c, 2c, 3c Expressed during limbFGFK mesenchymal cells and brain developmentFGF21 19.4 5.29 unknown at unknown at Expressed in liverFGFL (19.9 kDa) (4.59) time of printing time of printingFGF22 17.1 11.71 hair follicle- unknown at May be involvedFGFM (16.5 kDa) (11.58) keratinocytes time of printing in cutaneousdevelopment & repair,and hair developmentFGF23 25.3 8.95 renal proximal 3c Expressed in brain andFGFN (25.3 kDa) (9.64) epithelial cells thymus, regulatesphosphate homeostasis,mutant in hypophosphatemicrickets.144To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comTNF Superfamily NomenclatureTNF Superfamily – LigandsNomenclature NameTNFSF1ATNFSF1BTNFSF3TNFSF4TNFSF5TNFSF6TNFSF7TNFSF8TNFSF9TNFSF10TNFSF11TNFSF12TNFSF13TNFSF13BTNFSF14TNFSF15TNFSF18TNFSF20TNF Superfamily – ReceptorsNomenclature NameTNFRSF1ATNFRSF1BTNFRSF3/LTBR: (lymphotoxin beta receptor)TNFRSF4TNFRSF5TNFRSF6TNFRSF6BTNFRSF7TNFRSF8TNFRSF9TNFRSF10ATNFRSF10BTNFRSF10C: (decoy without an intracellular domain)TNFRSF10DTNFRSF11ATNFRSF11B(TNFRSF12)TNFRSF12ATNFRSF12LTNFRSF13BTNFRSF13CTNFRSF14TNFRSF16TNFRSF17TNFRSF18TNFRSF19TNFRSF19LTNFRSF21TNFRSF22TNFRSF23TNFRSF25TNFRSF26Note: This table is reflective of the current knowledge.Functional NamesTNF-alpha, Cachectin, DIF, TNFSF2TNF-beta, LT alpha, TNFSF1LT beta, TNFC, p33OX40 Ligand (OX40L), gp34, TXGP1CD40 Ligand (CD40L), IMD3, TRAP, gp39, CD154, HIGM1, hyper-IgM syndromeFas Ligand (FasL), Apo I Ligand (ApoI L), APTL, APT1LG1, CD95LCD70, CD27 Ligand, CD27LG (CD27L, CD27LG)CD30 Ligand (CD30L, CD30LG), CD1534-1BB Ligand (4-1BBL), CD Antigen 137 Ligand (CD137L)TRAIL, Apo-2 Ligand (Apo2L), TL2RANK Ligand (RANKL), TRANCE, OPGL, ODFTWEAK, DR3LG, APO3-Ligand (APO3L)APRIL, TALL2BAFF, BLyS, THANK, TALL1, DTLLIGHT, LTg, HVEM-Ligand (HVEM-L)TL1A, TL1, VEGIAITR Ligand (AITRL), TL6, GITRL Ligand (GITRL)BAFF, BLyS, THANK, TALL1, DTL, TNFSF13BFunctional NamesTNF Receptor Type I (TNFR1), TNFAR, TNF-R55, TNFR60, p55-R, p55TNFR, p55, CD120aTNF Receptor Type II (TNFR2), CD120b, p75, TNF-R, TNFR80, TNF-R75, TNFBR, p75TNFRTNFR2-RP, CD18, TNFR-RP, TNFCR, TNF-Receptor Type III (TNFR3)OX40, ACT35, TXGP1LCD40 (Receptor), p50, Bp50Fas, CD95, Apo-1, APT1DcR3, M68, TR6CD27, Tp55, S152CD30 , Ki-1, D1S166E4-1BB (Receptor), CD137, ILADR4, Apo2, TRAIL Receptor 1 (TRAILR1)DR5, TRAIL Receptor 2 (TRAIL-R2), KILLER, TRICK2A, TRICKBDcR1, TRAILR3, LIT, TRIDDcR2, TRUNDD, TRAILR4RANK (Receptor), ODFR, TRANCE ROsteoprotegerin (OPG), PRG, OCIF, TR1TNFRSF12 (bovine)TWEAK Receptor (TWEAKR), FN14DR3LTACIBAFFRHVEM, ATAR, TR2, LIGHTR, HVEANGFR, p75NTKBCMA, BCMAITR, GITR, GITR-DTROY, TRADE, TAJRELTDR6SOBa, Tnfrh2, 2810028K06Rik, DcTRAIL R2mSOB, Tnfrh1, DcTRAIL R1DR3, Apo-3, TRAMP, LARD, TR3, WSL-1, TNFRSF12TNFRH3INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se145

www.peprotech.com2011-2012 CatalogueTGF-β SuperfamilyNatural Antagonists/Name Synonyms Main Function Binding ProteinsINFORMATION & TECHNICAL SUPPORTTGF-β 1Differentiation inhibiting Regulates cell proliferation, growth, differentiation Follistatin, Follistatinfactor, cartilage-inducing and motility. Involved in adipogenesis, -like related genefactor chondrogenesis, embryogenesis, tissue remodeling, (FLRG), Decorin,wound healing and tumor formation.alpha-2macroglobulinTGF-β 2Glioblastoma-derived Regulates cell proliferation, growth, differentiation Decorin, alpha-2T-cell suppressor factor, and motility. Involved in adipogenesis, macroglobulinBSC-1, Cetermin,chondrogenesis, embryogenesis, tissue remodeling,Polyerginwound healing and tumor formation.TGF-β 3None Regulates cell proliferation, growth, differentiationand motility. Involved in adipogenesis,chondrogenesis, embryogenesis, tissue remodeling,wound healing and tumor formation.TGF-β 4Endometrial bleeding asso- Essential for left-right (L-R) asymmetryciated factor beta-4, (EBAF) determination of organ systems. Possible role inLefty preproprotein, LEFTA endometrial bleeding.Inhibin A Inhibin alpha & beta A Inhibits secretion of follitropin by the pituitarygland, regulates embryogenesis, osteogenesis,hematopoiesis, reproductive physiology andhormone secretion from the hypothalamic,pituitary and gonadal glands.Inhibin B Inhibin alpha & beta B Inhibits secretion of follitropin by the pituitarygland, regulates embryogenesis, osteogenesis,hematopoiesis, reproductive physiology andhormone secretion from the hypothalamic,pituitary and gonadal glands.Activin A Activin beta-A, Inhibin Regulates embryogenesis, osteogenesis, Follistatin,beta-1, FRP, (Follicle- hematopoiesis, reproductive physiology and Follistatin-likestimulating hormone- hormone secretion from the hypothalamic, pituitary related gene (FLRG),releasing protein),FSH- and gonadal glands. GASP-1, Cerberus,releasing protein, FSH-DAN, alpha2releasing factor, EDFmacroglobulin.(Erythroid differentiation factor)Activin AB Activin beta A & beta B Regulates embryogenesis, osteogenesis,hematopoiesis, reproductive physiology andhormone secretion from the hypothalamic, pituitaryand gonadal glands.Activin B Activin beta-B, Inhibin Regulates embryogenesis, osteogenesis,beta-2hematopoiesis, reproductive physiology andhormone secretion from the hypothalamic, pituitaryand gonadal glands.Activin C Activin beta-C, Inhibin Regulates embryogenesis, osteogenesis,beta-C, blastocyst B1 hematopoiesis, reproductive physiology andhormone secretion from the hypothalamic, pituitaryand gonadal glands.Activin E Activin beta-E, Regulates embryogenesis, osteogenesis,inhibin-beta Ehematopoiesis, reproductive physiology andhormone secretion from the hypothalamic, pituitaryand gonadal glands.BMP-2 BMP-2A Induces cartilage and bone formation, plays a Noggin, Chordin,role in cardiac morphogenesis.Follistatin,Follistatin-like relatedgene (FLRG), DAN,Cerberus, GremlinBMP-3 Osteogenin Induces cartilage and bone formation.146To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comTGF-β Superfamily continuedNatural Antagonists/Name Synonyms Main Function Binding ProteinsBMP-3B GDF-10 Biological function unknown, but may play a rolein differentiation of osteoblasts, augmenting BMP-2activity.BMP-4 BMP2B (BMP2B1, Induces cartilage and bone formation, involved in Noggin, Chordin,BMP2B2, Bmp2-rs1) mesoderm induction, tooth development, limb Chordin-like/formation and fracture repair.Neuralin/DAN,Cerberus, GremlinBMP-5 None Induces cartilage and bone formation. Noggin, Chordinlike/Neuralin/Ventroptin,Sclerostin/SOSTBMP-6 Vgr-1, Induces cartilage and bone formation. Noggin, Chordin-Vg-1-related proteinlike/Neuralin/Ventroptin Follistatin,Follistatin-likerelated gene (FLRG),Sclerostin/SOSTBMP-7 OP-1 (Osteogenic protein-1) Induces cartilage and bone formation, involved in Noggin, Chordin,calcium regulation and bone homeostasis. May act Follistatin-relatedas an osteoinductive factor responsible for epithelial gene (FLRG),osteogenesis.DAN, Cerberus,Sclerostin/SOSTBMP-8 BMP-8a, Induces cartilage and bone formation, involved inOP-2 (Osteogenic protein-2) calcium regulation and bone homeostasis. May actas an osteoinductive factor responsible for epithelialosteogenesis.BMP-8b None Stimulates cartilage and bone formation, implicatedin calcium regulation and bone homeostasis.BMP-9 GDF-2 May be involved in bone formation, regulates bloodglucose homeostasis, potential autocrine/paracrinemediator in the hepatic reticuloendothelial system,involved in chondrogenesis.BMP-10 None Plays a crucial role in trabeculation of theembryonic heart.BMP-11 GDF-11 Involved in the patterning of both mesodermal Follistatin,and neural tissues and in establishing the skeletal Follistatin-likemuscle. Acts globally to specific positional identity related gene (FLRG),along the anterior/posterior axis.GASP-1BMP-12 GDF-7, CDMP-3 Induces the formation of tendon and ligamenttissues.BMP-13 GDF-6, CDMP-2 Plays a role in cartilage homeostasis, involved in the Nogginembroyonic skeletal development, and formation ofa tendon-like tissue.BMP-14 GDF-5, CDMP-1 Essential for limb cartilage and limb-joint formation Noggin, DANin developing mice. Involved in the embryonicskeletal development.BMP-15 GDF-9B An oocyte-specific factor that regulates granulosacell proliferation and differentiation, and is essentialfor normal follicular growth.GDF-1 Embryonic growth/ May be involved in mediating cell differentiationdifferentiation factor events during embryonic development.INFORMATION & TECHNICAL SUPPORTContinued…Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se147

www.peprotech.com2011-2012 CatalogueTGF-β Superfamily continuedNatural Antagonists/Name Synonyms Main Function Binding ProteinsINFORMATION & TECHNICAL SUPPORTGDF-2 BMP-9 Implicated in bone formation.GDF-3 Vgr-2 Embryonal carcinoma stem cell-associated markerin vitro and in vivo.GDF-5 CDMP-1, BMP-14 Essential for limb cartilage and limb-joint formation Noggin, DANin developing mice, involved in the embryonicskeletal development.GDF-6 BMP-13, CDMP-2, Formation of a tendon-like tissue. Plays a role in Noggindynamo (zebrafish) cartilage homeostasis, and embryonic skeletaldevelopment.GDF-7BMP-12, CDMP-3, prostate Induces the formation of tendon and ligamentdifferentiation factor, tissues.MIC-1, PLABGDF-8 Myostatin (MSTN) Regulates skeletal muscle mass. Follistatin,Follistatin-likerelated gene(FLRG), GASP-1GDF-9 None Essential for normal follicular growth.GDF-10 BMP-3B, Biological function unknown, but may play a roleBIP (Bone-Inducing Protein) in differentiation of osteoblasts, augmentingBMP-2 activity.GDF-11 BMP-11 Involved in the patterning of both mesodermal and Follistatin,neural tissues and in establishing the skeletal Follistatin-likemuscle. Acts globally to specific positional identity related gene (FLRG),along the anterior/posterior axis.GASP-1GDF-15 PLAB, placental bone Possible mediator of placental control of embryonicprotein, prostatedevelopment, may act as an autocrine regulatorydifferentiation factor, molecule.NRG1, MIC-1(Macrophage Inhibiting Cytokine-1)GDNF ATF (Astrocyte derived Promotes dopamine uptake and survival andneurotrophic factor) morphological differentiation of midbrain neurons.Artemin ARTN Supports the survival of all peripheral ganglia suchas sympathetic, neural crest and placodally-derivedsensory neurons, and dopaminergic midbrainneurons.Neurturin NTN, NRTN Promotes the development and survival ofsympathetic and sensory neurons.Persephin PSP, PSPN Promotes the survival of ventral midbraindopaminergic neurons and motor neurons, andpromotes ureteric bud branching.LEFTY-1 LEFTYB, Protein Lefty B Essential for left-right asymmetry of organ systems.LEFTY-2 LEFTYA, TGF-b 4, Essential for left-right asymmetry of organ systems.Protein Lefty AAMH MIS, Muellerian Causes regression of the Muellerian duct,inhibits(anti-Muellerian inhibiting substance the growth of tumors derived from tissues ofhormone)Muellerian duct origin.Dorsalin dorsalin-1, DSL-1 Regulates cell differentiation within neural tube.(chick)NODAL None Essential for mesoderm formation and Cerberussubsequent organization of axial structures.Note: This table is reflective of the current knowledge.148To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comAntagonists of TGF-β LigandsNatural TGF-β Antagonists Structural Features Contained in the Known TGF-β BindingAntagonist Polypeptide (M.W.)PartnersNoggin unique Noggin cysteine knot (26 kDa) BMP-2,-4,-5,-6,-7,-13/GDF-6,-14/GDF-5Chordin 4 CR/VWCC (Chordin) domains, BMP-2,-4,-73 SOG repeats (102 kDa)Chordin-like/Neuralin/Ventroptin 3 Chordin domains (51 kDa) BMP-4,-5,-6Follistatindomains (38 kDa)3 cysteine-rich Follistain (FS) and 3 kazal activin, BMP-2,-4,-6,-7, myostatin/GDF-8,GDF-11, TGFβ-1Follistatin-like related gene 2 FS and 2 kazal domains (28 kDa) activin, myostatin/GDF-8, GDF-11, TGFβ-1(FLRG)GASP-1 1 Wap, 1 FS, 1 kazal, 1 IG-like, myostatin/GDF-8, GDF-112 kunitz, 1 netrin domains (63 kDa)Follistatin-related protein 1 FS, 1 CR/VWRC, activin, BMP-2,-6,-7(FSRP)2 EF-hand domains (35 kDa)DAN unique DAN cysteine knot (19 kDa) BMP-2,-4,-7, -14/GDF-5Cerberus DAN-like cysteine knot (30 kDa) BMP-2,-4,-7, activin, nodalGremlin DAN-like cysteine knot (21 kDa) BMP-2,-4,-7Sclerostin/SOST unique Sclerostin cysteine knot (24 kDa) BMP-5, -6Decorin multiple leucine-rich repeats (40 kDa) TGFβ-1, -2alpha-2 macroglobulin multiple proteinase inhibitor domains TGFβ-1, -2 , activin, inhibin(163 kDa)Note: This table is reflective of the current knowledge.INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se149

www.peprotech.com2011-2012 CatalogueGeneral Characteristics of Plasma LipoproteinsLP Particle Size Density (g/ml)* TG/CE Ratio* L/P Ratio* Associated ApoproteinsCM 1000nm

PEPROTECH ®www.peprotech.comGeneral AbbreviationsAA (aa) Amino AcidAAP Antigen-Affinity PurifiedABTS azino-diethylbenzothiazoline-sulfonateAEC 3-amino-9-ethylcarbazoleAP Alkaline PhosphataseAR Antigen RetrievalbME beta-MercatoethanolBSA Bovine Serum AlbuminBt BiotinylatedCHO Chinese Hampster Ovary (cells)ConA Concanavalin ADa DaltonsDAB “3,3' Diaminobenzidine”DI DeionizedDMEM Dulbecco’s Minimum Essential MediumDMSO Dimethyl SulfoxideDTT DithiothreitolED50 50% Effective DoseEDK ELISA Development KitECM Extra Cellular MatrixEIA Enzyme ImmunoassayEIER Enzyme-Induced Epitope RetrievalELISA Enzyme-Linked Immunosorbent AssayEU Endotoxin UnitES Embryonic Stem CellsFACS Fluorescence-Activated Cell SorterFBS Fetal Bovine SerumFCS Fetal Calf SerumFFPE Formalin-Fixed Paraffin-EmbeddedFLISA Fluorescence-linked immunosorbent assayGt GoatHCl Hydrochloric acidHEK293 Human Endothelial Kidney CellsHIER Heat-Induced Epitope RetrievalHoAc Acetic AcidHPLC High-Performance Liquid ChromatographyHRP Horseradish PeroxidaseHSA Human Serum AlbuminHSC Hematopoietic Stem CellsHUVEC Human Umbilical Venous Endothelial CellsICCIgGIgEIHCIUkDaLALLPSMAbMSDSMWMSCNaP (NaPO4)NFDMNKNMNSCO.D.PAbPAGEPCRPBLPBSPBSTPBMCPHApIPIERRbRIARTSDSTBSTFATMBWBWFIWHOImmunocytochemistryImmunoglobulin GImmunoglobulin EImmunohistochemistryInternational UnitkilodaltonsLimulus Amoebocyte Lysate assayLipopolysaccharideMonoclonal AntibodyMaterial Safety Data SheetMolecular WeightMesenchymal Stem CellsSodium PhosphateNonfat Dry MilkNatural Killer CellsNitrocellulose MembraneNeural Stem CellsOptical DensityPolyclonal AntibodyPolyacrylamide gel electrophoresisPolymerase Chain ReactionPeripheral Blood LymphocytesPhosphate Buffered SalinePBS with 0.1% TWEEN-20Peripheral Blood Mononuclear CellsPhytohemagglutininIsoelectric PointProtease-Induced Epitope RetrievalRabbitRadioimmunoassayRoom TemperatureSodium Dodecyl SulfateTris Buffered SalineTrifluoroacetic Acid“3,3',5,5'-tetramethylbenzidine”Western BlotWater Force InjectionWorld Health OrganizationINFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se151

www.peprotech.com2011-2012 CatalogueCytokines - Technical FAQINFORMATION & TECHNICAL SUPPORTBefore releasing our cytokines and cytokine productsfor sale they are thoroughly analyzed to ensurethe following qualities:1. Authenticity: by N-terminal sequence analysisand, when possible, by SDS-PAGE, RP-HPLC, and Mass Spectrometry analyses versusstandards.2. Purity: by SDS-PAGE and RP-HPLC analysis3. Biological Activity: by the relevant in vitro or in vivobioassay.4. Protein content: by UV spectroscopy, SDS-PAGEanalysis and, when possible, by HPLC analysis versusthe calibrated standard solution.5. Endotoxin contamination: by kinetic LAL method.6. Microbiological contamination: by membrane filtrationmethod; protein solutions are sterile-filtered priorto vialing.The relevant information relating to each productappears on the Certificate of Analysis that is shippedwith the product. Please read this information carefullyto obtain useful instructions for reconstitutionand storage. If, after reading the data sheet, you needadditional information, please review the followingset of questions and answers, or call our technicalservice department at 800-436-9910.1. What should I know about the stability of your proteinproducts?Unless otherwise mentioned on the product informationsheet, all of our products are formulated insuch a manner that the lyophilized proteins are verystable at room temperature. However, we recommendstoring lyophilized products at -20 o C.For reconstituted solutions of most products, werecommend short-term storage at 4 o C. For longerterm storage the protein solution should be storedwith a carrier protein (eg. 0.1%BSA) in workingaliquots and stored frozen at -20 o C. Please keep inmind that every freeze/thaw cycle may cause somedenaturation of the protein.2. Why can't I see the protein pellet in the vial?Unlike many protein products available on themarket, PeproTech products are not formulatedwith carrier protein or other additives (e.g., BSA,HSA, sucrose, etc.) and are often lyophilized witha minimum amount of salt. As a result, the smallamounts of protein can be deposited on the vial duringlyophilization as a thin and sometimes invisiblefilm. Before opening, we recommend centrifugingeach vial in a microcentrifuge for 20-30 seconds todrive any protein that may be lodged in the cap or onthe side to the bottom of the vial. Our quality controlprocedures assure that each vial contains the correctamount of product.3. What is the relationship between the specific activityexpressed as an ED 50and as units/mg?The ED 50is defined as the cytokine concentrationat which the activity is 50% of the maximumresponse. This method of expressing potency shouldonly be used for cytokines whose dose-responsecurves are sigmoidal in shape. The formula forconverting the activity as an ED 50in ng/ml to specificactivity in units/mg is:1 x 10 6 specific activity (units/mg)ED 50(ng/ml)=4. Which cytokines show cross-species activity?With a few exceptions, most human cytokines areactive on mouse cells. Many mouse cytokines areactive on human cells, but may show lower specificactivity than the corresponding human cytokine.A few human cytokines, such as IL-7, even exhibithigher specific activities on mouse cells than do thecorresponding mouse cytokines. The interferons, GM-CSF, IL-3, and IL-4 are known to be species-specificwith very little, if any, activity on non-homologouscells. In contrast, the FGF's and neurotrophins arevery highly conserved and show excellent activity oncells of other animal species.152To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comAnimal-Free Media Supplement Kits- Technical FAQ1. What is chemically defined serum freemedia?Chemically defined media include onlycomponents of known molecular structuresand concentrations.2. Are PeproTech’s Serum-Free Cell Culturemedia supplement kits protein-free?Yes, PeproTech’s Serum-Free Cell Culturemedia kits are protein-free, as well asanimal-free.3. What are the advantages of usingchemically-defined Serum-Free media?The advantages include: lot-tolotconsistency and reproducible cellgrowth; greater protein yield (for proteinproduction applications) vs. using serum,consistent media performance, loweredrisk of contamination with viral and otheradventitious infectious agents, reduceddownstream purification challenges (forprotein production applications), eliminationfor the need to prescreen serum lots, andsimplification of any regulatory or otherdocumentation requirements.4. Can you provide the certifications for theseanimal-free components?Yes, please contact QC/Technical services.5. Where can I find formulations for these kits?Formulations for these kits are proprietaryto PeproTech, Inc.6. Can I use DMEM instead of DMEM/F12?DMEM/F12 medium is the mixture ofhalf DMEM and half F12 medium, thereforeDMEM/F12 has much more nutritionthan DMEM alone. PeproTech has nottested DMEM at this time. Therefore, it isrecommended that DMEM/F12 is used withPeproTech supplements.INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se153

www.peprotech.com2011-2012 CatalogueAntigen Affinity Purification of AntibodiesINFORMATION & TECHNICAL SUPPORTCytokines are signaling proteins necessary for cell- body production. Consequently, the sera obtained fromto-cell communication throughout the body. In accordancewith their role in preserving vital biological func-I-4) typically contain minute amounts of cytokine-spec-host animals after immunization with cytokines (Figuretions, the amino-acid sequence of homologous cytokines ific antibodies. Isolation of these antibodies by stanfromdifferent mammalian species is generally highly dard purification procedures (e.g. ion-exchange chromatography)is tedious and ineffective. On the other conserved, rendering them poor immunogens for anti-hand,Figure I-1 - Antigen Affinity Purification of Antibodies(A.) Preparation of separation resin. (B.) Specific binding of Antibody to the resin. (C.) pH elution of the antibody from the affinity column.154To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.compurification protocols which exploit the binding affinity contains less than 0.2% of the desired antibody. The largeof antibodies to certain bio-molecules have yielded much quantity of unrelated IgGs found in these preparationsbetter results. For example, Protein A and Protein G are not only interferes with quantification of the relativebacterial (Staphylococcus)-derived proteins that possess amount of the cytokine-specific antibody, but also conhighbinding affinity toward the F c (Fragment crystallizable)region of immunoglobulins (IgGs), and when atbodyis used in analytical procedures such as ELISA,siderably increases the background noise when the antitachedto a solid-support matrix, enable efficient separationof IgGs from other serum constituents. Protein A/G ting. A superior method for isolating specific polyclonalneutralization, immunohistochemistry, and Western blot-affinity purification of serum-IgGs typically enriches the antibodies from antiserum is by affinity chromatography,which exploits the specificity of antibody-antigendesired antibody by more than 100-fold. Yet, in the caseof anti-cytokine antibodies, the total IgG fraction often interactions (Figure I-1). Here, the separation mediumFigure I-2Schematic illustration of column chromatography setup for antigen-affinity purification of antibodies.PB{ }INFORMATION & TECHNICAL SUPPORTNOTE: Elution is titrated with1M NaOH to pH 7.2.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se155

www.peprotech.com2011-2012 CatalogueAntigen Affinity Purification of Antibodies Continuedconsists of a solid-support resin to which the antigen isattached through stable covalent bonds. The immobilizedantigen is then used in a column chromatography setupto selectively capture antigen-specific antibodies, whileother serum proteins and unrelated immunoglobulins arewashed away (Figure I-2). The antigen-bound antibod-ies can be eluted from the column by an acidic solution(Figure I-3), which is promptly brought back to physiologicalpH to prevent acid-catalyzed antibody denaturation.This method typically yields >95% pure specificantibodies (Figure 1-5).INFORMATION & TECHNICAL SUPPORTFigure I-3 - A typical Antigen Affinity Purification ChromatogramFigure I-4 - General Immunization Schedulefor Polyclonal Antibody Production in RabbitsDay Procedure Antiserum Collected1 Prebleed 3mL1 Initial Immunization (250ug) -21 Immunization (125ug) -31 Titer Test Bleed 3mL42 Immunization (125ug) -49 Production Bleed 10-25mL56 Production Bleed 10-25mL63 Immunization (125ug) -70 Production Bleed 10-25mL77 Production Bleed 10-25mL84 Immunization (125ug) -91 Production Bleed 10-25mL98 Production Bleed 10-25mLFigure I-5 - SDS-PAGEanalysis of rabbit anti-human IL-8polyclonal antibodies (RbαhIL-8)Lane 1: Molecular weight markersLane 2: 2µg of RbαhIL-8 (unreduced)Lane 3: 2µg of RbαhIL-8 (reduced)Approx.M.W. (kDa)200.0116.397.466.355.436.531.021.514.46.01 2 3156To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comAntibody FAQ1. Does PeproTech test for endotoxin in their antibodies?PeproTech does test their antibodies forendotoxin using the kinetic LAL method.Please contact Technical Support for moreinformation.2. Which isotype are PeproTech’s polyclonal antibodies?The polyclonal antibodies that PeproTechmanufactures are IgG antibodies.3. How are your antibodies purified?All polyclonal and biotinylated polyclonalantibodies are antigen-affinity purified. Monoclonalantibody purification varies by product;please contact Technical Support for ˇmoreinformation.4. Can you tell me what epitope your antibodybinds to?PeproTech does not perform epitope mappingat this time. As a general guideline, polyclonalantibodies will naturally bind to more epitopesthan monoclonal antibodies since they are IgGantibodies.5. Have PeproTech’s antibodies been tested in neutralizationassays?Neutralization testing is performed on a lotto-lotbasis for each antibody. The resultsof this testing can be found on the product’scorresponding data sheet.6. Are PeproTech’s antibodies suitable for usein ELISA and Western Blot applications?PeproTech’s antibodies are suitable for use inELISA and Western Blot assays; please see ourindividual ELISA and Western Blot F.A.Q.sections for more information regarding theseapplications.7. What information should be known aboutthe stability of your antibody products?PeproTech’s antibodies are lyophilized fromPBS. As such, they are stable at roomtemperature for at least 1 month. For longerperiods, we recommend storing the lyophilizedproducts at -20 ° C.For reconstituted solutions of the antibodies,we recommend short-term storage at 4 ° C.For long-term storage the antibody solutionshould first be aliquoted (to avoid more than onefreeze/thaw cycle) and stored frozen at -20 ° C.Frozen aliquots of this antibody solution arestable for at least 1 year when kept at -20 ° C.8. Do PeproTech’s antibody products contain anycarrier proteins or other additives?No, PeproTech does not formulate itspolyclonal, biotinylated polyclonal, ormonoclonal antibodies with additives or carrierproteins.9. Will PeproTech’s antibodies work inimmunohistochemistry andimmunocytochemistry applications?All antibodies that have been tested so farhave been found to be suitable for theseapplications. However, we are still gatheringdata for more of our antibodies. Please contactTechnical Support for more information.10. Will PeproTech antibodies recognize targetproteins sold by other vendors?PeproTech’s antibody products have highbinding affinity towards the natural and/orrecombinant versions of the correspondingproteins. However, due to lack of authenticitysometimes found in other vendors’ proteins, wecannot guarantee that our antibodies willperform as well with these proteins.11. Will PeproTech’s antibodies recognize targetprotein in complex biological fluids such asblood or serum?Yes. However, for samples that have a highcontent of interfering agents the recognition willbe less efficient and a higher background, orreduced signal to noise ratio, may be seen.INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se157

www.peprotech.com2011-2012 CatalogueELISAINFORMATION & TECHNICAL SUPPORTEnzyme-linked immunosorbent assay (ELISA) is isotopes and expensive counting devices. In recent years,a laboratory technique for quantifying the concentration ELISA has gained popularity in both research and clinicallaboratories, and has become an important diagnos-of specific antigens or antibodies in cell lysates, sera, orother complex solutions. In its simplest format, called tic test for many infectious diseases, including the hudirectELISA, a solid-phase binding support such as a man immunodeficiency virus (HIV).polystyrene plate is used to capture the tested antigen, A number of different enzymes are suitable for thewhich is then detected with the aid of a specific enzymelinkedantibody (E-Ab). The detection protocol involves horseradish peroxidase, and carbonic anhydrase. Al-preparation of E-Abs, including alkaline phosphatase,conversion of a colorless substrate solution into an intense-coloredproduct that can be measured directly in enzymes to antibodies through a coupling agent such asthough E-Abs can be obtained by covalently bindingthe reaction plate. The increase in optical density during glutaraldehyde, this method is not commonly used, bethereaction is recorded at different time points for sev- cause it often yields products with impaired immunologicaland/or enzymatic activity. A superior method toeral sample dilutions, and the collected data is then usedin conjunction with a standard curve to calculate the concentrationof the antigen in the original sample (Figure ing affinity of avidin for biotin, as depicted in Figure IIpreparefully active E-Abs is by exploiting the high bind-II-1). Although the sensitivity and specificity of ELISA 2. The biotinylated antibody used in this method containson average 6-8 biotin moieties, which due to theirare comparable to those of radioimmunoassay (RIA),ELISA has the advantage of not requiring radioactive small size do not interfere with the interaction betweenFigure II - 1 - Sample Standard Curve - Human EGF ELISA158To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comFigure II - 2 - Steps in the production of enzyme-linked antibody,through Avidin-Biotin interaction.(A.) Biotinylation of an Antibody. (B.) Avidin - Enzyme Conjugation.(C.) In Situ formation of enzyme-linked Antibody via noncovalent interaction between Avidin and Biotin.22 3INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se159

www.peprotech.com2011-2012 CatalogueELISA continedINFORMATION & TECHNICAL SUPPORTthe antibody and the captured antigen. Once unboundantibody has been rinsed from the plate, the biotin moietiesare used in-situ to form stable complexes with avidin-enzymeconjugates. A major limitation of the directELISA format is that certain antigens (e.g. low molecularweight cytokines) bind poorly to polystyrene plates,and therefore, cannot be measured accurately by the assay.To by-pass this limitation, the plate can be pre-coatedwith a specific antibody that recognizes the tested antigenthrough epitopes that are not used by the E-Ab. Usinga matched pair of antigen-specific antibodies, one forcapturing and one for detection, significantly improvesboth the sensitivity and specificity of the assay. This format,called Sandwich ELISA, enables detection of picogramquantities of antigens and is the preferred methodfor measuring cytokines and hormones in biologicalsamples (Figure II-3).Figure II - 3 - Schematic presentation of Sandwich ELISA protocol.A. Prepare freshsolution of antigenspecificantibody(capture antibody)in PBS (1.0µg/ml),and promptly add100µl aliquots toeach well. Seal theplate and incubateat room temperatureovernight or at 37˚Cfor 2-4 hours. Whenincubation time iscomplete, washaway unboundantibody (i.e.aspirate and wash4x).B. Add freshsolution of blockingagent (e.g. BSA) andincubate for at least1 hour. Whenincubation is over,wash away theblocking agent.Note: Inefficientblocking of nonspecificbindingmay lead to highbackground noise. Ifthis is observed,increase theincubation time ortry other blockingagents.C. Add freshlyprepared solutionsof standards,samples or controlsand incubate for > 2hours. Wash thewells 4x.BBB BBD. Add freshlyprepared solution ofbiotinylated antigenspecificantibody(detection antibody),and incubate for2 hours. Wash thewells 4x.BBB BBE. Add a solution ofavidin-peroxidaseconjugate andincubate for 30minutes. Wash thewells 4x.BBB BBF. Add substrate(ABTS) solution andread the developingoptical density at 5minute intervalsusing an ELISA platereader set at 405nmwith a 650 nmwavelengthcorrection.Note: Blank opticaldensity (O.D.)readings should notexceed 0.2 O.D.units.Note: Unless otherwise specified, the ELISA protocol is conducted at ambient temperature.160To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comELISA FAQ1. How many plates can I run with one ofPeproTech’s ELISA Development Kits (EDKs)?One EDK contains enough material to run 10standard plates at 100 wells each. Thiscorresponds to 1,000 ELISA plate wells.2. What is the stability of the avidin-HRPincluded in PeproTech’s ELISA Development Kit?The avidin-HRP included in the kit is stable forup to 1 month at 2-8˚C, and for up to 2 years at-20˚C.3. How can I find cross reactivity information for akit?PeproTech performs in-house cross reactivitytesting on its ELISA Development Kits. Theresults that have been collected from this testingare located on the kit’s data sheet.4. Is there any step of the ELISA protocol that canbe left over the weekend?The plate may be coated with the captureantibody on Friday, left at 4˚C over the weekend,and resumed on Monday. Please note: changingincubation times may cause results to varybetween plates.5. Are PeproTech’s EDKs suitable to use with allsample types?Although PeproTech has not tested all of its kitsin every matrix available, they should be suitablefor use in, but not limited to: serum, plasma, cellculture supernatant, urine and saliva.6. Is a stop solution necessary to stop thereaction?A stop solution is not needed when usingavidin-HRP + ABTS. In general, reliable standardcurves are obtained when either O.D. readings donot exceed 0.2 units for the zero standard concentrations,or 1.2 units for the highest standardconcentration.If a stop solution is desired, 1% sodium dodecylsulfate (SDS) may be used to end the reaction.Stop solutions are not used in PeproTech’slaboratory, though literature has shown that SDS isthe optimal stop solution for ABTS.7. Can I use TMB with PeproTech’s EDKs?PeproTech’s EDKs are optimized using ABTSand are therefore best used in conjunction withthis substrate. The kit can still be used in combinationwith TMB, but only after some adjustmentshave been made:• The avidin-HRP provided in the kit cannot beused with TMB; streptavidin must be purchasedseparately.• Dilutions of streptavidin will need to beoptimized.• A stop solution is generally needed when usingstreptavidin + TMB. Refer to manufacturer’s datasheet.• The TMB reaction time, prior to the addition ofstop solution, will need to be optimized.• The plate is to be read at 450nm with a correctionat 650nm when using recommended plates.8. Why is D-mannitol added to the EDKcomponents?D-mannitol is added to the EDK components inorder to aid in protein/antibody visualization. Itdoes not alter ELISA results.9. Can I use the curve on the EDK data sheet as mystandard curve?A separate standard curve must be run on eachELISA plate. In other words, the curve from oneplate cannot be used for a different plate. Thecurve that PeproTech provides on the EDK datasheet is for demonstration purposes only, asachieved in PeproTech’s laboratory.10. How does PeproTech generate their standardcurve?When an ELISA is run in PeproTech’s lab, aMolecular Devices ® plate reader and theSOFTmax ® PRO software are used. This programuses the values that are received and generates a4-parameter curve. Here is the equation that theprogram uses:4-P Fit:x = concentration (pg/ml)y = O.D. (405nm – 650nm)A, B, C, & D correspond to the 4 parameters.**For more detailed information regarding the parameters,please contact the Technical Support Department.INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se161

www.peprotech.com2011-2012 CatalogueELISA Troubleshooting GuideWhen trouble shooting, prepare all fresh buffers. Sterile filter buffers containing BSA and allow all reagents to reachroom temperature prior to use.Problem Possible Source SuggestionINFORMATION & TECHNICAL SUPPORTHigh Background/ Non-specific binding of antibody • Modify blocking buffer used (e.g. substituteŇon-specific Colorcasein for BSA). Do not wash after blockingDevelopmentstep; dump, blot & go directly to the next step.Plate not washed sufficiently• Be sure all wells are filled with buffer duringˇevery wash step. Be sure washing apparatus isˇworking properly. Wash 3-5x between steps.ˇDo not add additional washes. After final wash,ˇblot plate forcefully on paper towel to removeˇresidual buffer. Be sure the correct amount ofˇ Tween was added to the wash solution (0.01-ˇ0.1% recommended).ˇ Contaminated Buffers • Prepare all buffers fresh (within 7 days of use)ˇand sterile filter.ˇ Incubation temperature too high • Incubate at room temperature (25ºC) throughoutˇwhole procedure.ˇ Incubation time too long • Reduce incubation time.ˇ Contamination of the substrate • Change pipette tips between reagents and useˇ with the conjugate or contamination separate reservoirs. Ideally, wash bufferof blank wells with positive control should be aspirated (pouring/dumping may leadˇto cross contamination).ˇ Concentration of detection antibody • Check calculations or try further dilutions.or avidin-HRP is too highˇ Substrate exposed to light prior to use • Keep substrate in the dark until ready toˇdispense into wells.ˇ Wrong filter used when taking readings • Wavelength should be 405nm for ABTS (450 forˇTMB) with a 650nm wavelength correctionˇwhen using recommended plates.ˇ Read beyond time needed for development • Read at 5 minute intervals in order to monitorˇblank O.D. readings.Weak/No Color A reagent or a step of the procedure • Check protocol and follow steps carefully.Development omitted by mistakeˇ Detergent concentration in wash buffer • Remove or decrease detergent (0.01-0.1%ˇ too high recommended).ˇ Plate washed inadequately • Decrease number of washes between steps,ˇespecially if not using automated or semi-ˇautomated washer. 1-2 washes may suffice.ˇ Enzyme inhibitor present in samples • Sodium azide inhibits peroxidase activity.ˇ Wrong incubation time or temperature • Check and follow protocol recommendations.ˇPlace plates in an incubator during incubationˇperiods to avoid temperature fluctuations (set toˇ25ºC).162To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comProblem Possible Source Suggestionˇ ˇWeak/No Color Wrong substrate volume added to wells • Check pipette; use calibrated pipettes.ˇ Ďevelopment Wrong enzyme-substrate system used • Check manufacturer specifications: avidin-HRPˇ ˇ(continued)+ ABTS/ streptavidin + TMB.ˇ Conjugate or substrate inactive • Test activity in another system and checkˇexpiration dates.ˇ Incorrect storage of components • Store all components as recommended on data sheet.ˇ Wrong filter used when taking readings • Wavelength should be 405nm for ABTS (450 forˇTMB) with a 650nm wavelength correctionˇwhen using recommended plates.Poor Standard Curve Incorrect preparation of standard • Reconstitute standard as suggested on dataˇsheet. Dilute only with recommended diluentˇbuffer unless alternative buffer has been optimized.ˇ Dilutions made too early • Prepare reagents immediately prior to use andˇadd to wells promptly.ˇ Capture Antibody did not bind • Use ELISA-suitable plates (i.e. Nunc MaxiSorpˇProd. # 439454); not tissue culture plates.ˇ Inefficient washing • Be sure wash apparatus is working properly (i.e.ˇdistributing even volumes into each well). Beˇsure wells are empty after aspiration, yet beˇsure to fill wells in a timely manner.ˇ Pipetting error • Dispense quickly and identically into the side ofˇeach well. Use calibrated pipettes.ˇ• In general, reliable standard curves are obtainedˇ Read beyond time needed for development when O.D. readings do not exceed 0.2 forˇblanks or 1.2 for the highest standard concentration.ˇ Incorrect storage of components • Store all components as recommended on dataˇsheet. Do not allow reconstituted reagents toˇstay at room temperature for excess time.Poor Precision Insufficient mixing of reagents • Ensure adequate mixing of reagents before pipetting.ˇ Inefficient washing • Be sure wash apparatus is working properly (i.e.ˇdistributing even volumes into each well). Beˇsure wells are empty after aspiration, yet beˇsure to fill wells in a timely manner.ˇ Pipetting error • Dispense quickly and identically into the side ofˇeach well. Use calibrated pipettes.ˇ Reused materials • Change pipette tips between samples, reservoirsˇbetween reagents, and plate sealers betweenˇincubation periods.ˇ Wells have been scratched by pipette • Use caution when dispensing and aspirating.ˇtips or washing apparatusˇ Precipitate in samples • Centrifuge vials prior to use.ˇ Unclean wells or debris • Inspect wells and remove debris prior to use.ˇWipe bottom of plate to remove any debris orˇfingerprints prior to reading.INFORMATION & TECHNICAL SUPPORTEdge Effects Evaporation • Use plate sealers between each step.ˇ Uneven temperature • Place plates in an incubator during incubationˇperiods to avoid temperature fluctuationsˇ(set to 25ºC). Do not stack plates.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se163

www.peprotech.com2011-2012 CatalogueWestern Transfer (Western Blot)INFORMATION & TECHNICAL SUPPORTWestern Transfer, also known as Western Blotting,is a rapid immunoblotting technique for identifyingthe presence of a particular protein in a complexmixture of proteins such as cell lysates or sera. Thetechnique exploits both the efficiency of SDS-PAGE toseparate a mixture of proteins into distinct protein bands,and the ability of immunochemical reagents to interactspecifically with a given protein antigen.A typical Western-transfer protocol (e.g. for thedetection of protein X) includes the following steps:(1) The proteins in the tested solution are separated intodistinct bands by SDS-PAGE, (2) The size-separatedproteins are transferred from the polyacrylamide gel toa nitrocellulose membrane (or another suitable matrix).(3) The membrane containing the protein bands is seriallyincubated with: (a) a suitable blocking reagent toprevent non-specific protein binding, (b) a wash solutionto rinse any unbound blocking reagent, (c) a probingantibody (anti-protein-X antibody) that forms a specificimmune complex with Protein-X, (d) additionalwash solution to remove any unbound antibody, (e) anenzyme-linked antibody that binds specifically to theF cregion of the anti-Protein-X antibody, (f) additionalwash solution to remove any unbound enzyme-linkedantibody, and finally, (g) a substrate solution, which inthe presence of the enzyme, yields an insoluble, coloredproduct that precipitates at the site of the immunecomplex, thereby rendering the Protein X band visible(Figure III-1).Typical Western Transfer andDevelopment Protocol• *Western Transfer Buffer (1/2 x TOWBIN)Tris Base..............1.45g (12mM finalconcentration)Glycine................7.2g (96mM finalconcentration)Methanol.............200mL (20% finalvolume)diH 2O...................add to 1.0L final volume• Antigen specific probing antibody (PrimaryAntibody)• Secondary antibody (Donkey-anti-probingantibody species conjugated to AlkalinePhosphatase)• Commercially available alkaline phosphataseconjugate substrate kitTransfer Protocol1. Load the protein sample onto a 4-20% Tris-Glycine polyacrylamide gel and run until desiredresolution is achieved. (The electrophoresis can befollowed by using pre-stained molecular weightmarkers).Continued…Figure III-1 - Schematic presentation ofWestern Transfer Protocol-+Materials Needed• SDS-PAGE apparatus and accessories• Submerged Western Transfer Cassette andaccessories• A nitrocellulose membrane, approximatelythe size of the gel, presoaked in WesternTransfer Buffer* for five minutes. (Note:The membrane should be handled withgloves and clean forceps to avoidcontamination with extraneous proteins.)164To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.com2. Set up the Submerged Western Transfer Cassetteas described below and in Figure III-2:a. Submerge the open Transfer Cassette cathodeplate onto the tray pre-filled with WesternTransfer Buffer.b. Place a sponge support pad onto the cassetteand remove the air bubbles by gently rolling aPasteur pipette over the pad.c. Place a piece of blotting paper onto the spongesupport pad.d. Remove the gel from the electrophoresisplates, cut off approximately the bottom 3mmof the gel so that the membrane can be laidflat against the gel, and place the gel over theblotting paper. Expel air bubbles as before.e. Carefully place the pre-soaked nitrocellulosemembrane onto the gel and expel air bubbles.Ensure that the membrane remains directlyover the gel before proceeding.f. Place a second piece of blotting paper ontothe nitrocellulose membrane and remove airbubbles.g. Place a sponge support pad onto the secondpiece of blotting paper and remove airbubbles.h. Gently close the cassette by placing the anodeplate over the exposed pad.3. Carefully place the assembled cassette into thetransfer tank containing Western Transfer Buffer upto the “pre-fill” level and adjust the buffer level, asneeded, after the addition of the cassette.4. Connect the assembled apparatus to anelectrophoresis power supply and run forapproximately 1.5 hours at a constant current of400mA.Development (Immunostaining) Protocol1. After the transfer is complete, incubate themembrane in blocking solution (3% Nonfat DryMilk in diH 2O) for 30 minutes with gentleagitation on an orbital shaker.2. Wash the membrane three times with TBST (TBS,pH 7.2 with 0.1% TWEEN-20) in a clean tray onan orbital shaker; each wash lasting 5-10 minutes.3. Dilute the probing (primary) antibody in TBST toa volume of 50ml (approximate final concentrationof 0.20µg/ml) and incubate the membrane in theantibody solution for one to four hours at roomtemperature. (The optimum incubation timedepends on the antibody/antigen binding affinityand must be pre-determined for each antibody.)4. Wash the membrane three times as in step #2.5. Dilute the secondary antibody in TBST accordingto the manufacturer’s specification. Incubate themembrane in a clean tray containing 50ml ofdiluted secondary antibody for one hour at roomtemperature on an orbital shaker.6. Wash the membrane three times as in step #2.7. Color development requires the use of acommercially available (e.g. Bio-Rad or Sigma)alkaline phosphatase conjugate substrate kit.Follow the manufacturer’s instructions.8. After the bands become clearly visible, stop thecolor by placing the membrane in a tray containingdiH 2O for at least ten minutes.Figure III-2 - Submerged WesternTransfer CassetteINFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se165

www.peprotech.com2011-2012 CatalogueWestern Transfer FAQINFORMATION & TECHNICAL SUPPORT1. How long will the immunostaining take whenPeproTech’s Western Transfer protocol isfollowed?The Western Transfer process will takeapproximately 6 hours from transfer of proteinsto visualization of bands.2. What type of molecular weight markershould be used?PeproTech uses Invitrogen’s Multi-Mark prestainedmolecular weight marker for use in allWestern Transfers performed in-house, althoughthis molecular weight marker does not have tobe utilized. However, it is necessary that a prestainedmolecular weight marker is used whennot utilizing the ECL detection method.3. Is the addition of a positive control necessary ina Western Transfer?Yes, in order to know exactly how the proteinof interest will visualize on the Western Transferyou must add a positive control to your gel.When using PeproTech’s antibodies in a WesternTransfer, we recommend using the correspondingPeproTech recombinant protein, which was theimmunizing antigen for the antibody of choice.4. Does the color development system recommendedin PeproTech’s Western Transferprotocol have to be used?No. There are many different color developmentsystems that can be used for the visualizationof your Western Transfer. However, thesystem that is chosen must be compatible withthe enzyme conjugate being used. PeproTechuses an alkaline phosphatase linked secondaryantibody. NBT/BCIP is suitable for use with thisenzyme, and is therefore used for the visualizationof our Western Transfers.5. Is agitation of the membrane essential during theincubation periods?Yes, it is essential that the membrane beagitated during the incubation periods. If themembrane is not agitated the antibodies,blocking buffer, and washing buffer may notaffect the membrane evenly and can createsplotchy or patchy background. It can also limitthe detection of the proteins by the antibodies.6. Why are some Western Transfer resultsstronger than others when using different lots ofthe same antibody?Due to the nature of polyclonal antibodies,variability may be seen from lot to lot.7. Is it necessary to include the filter paperduring the protein transfer step?Yes, it is necessary to include the filter paperas a barrier in the protein transfer step. This filterpaper helps protect the gel and membrane fromany possible damage resulting from directcontact with the sponges, yet does not interferewith the electric current.8. Can an enzyme-conjugated primary antibody beused in a Western Transfer rather than using aprimary/secondary antibody system?Yes, an enzyme-conjugated primary antibodycan be used instead of utilizing a primary/secondary antibody system in your WesternTransfer. However, by using a labeled secondaryantibody that recognizes the antigen specificprimary antibody, there will be an amplificationof the signal seen in a Western Transfer whencompared to using the enzyme-conjugatedprimary antibody alone.166To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comWestern Transfer Troubleshooting GuideProblem Possible source SuggestionWeak/No Signal Insufficient amount of protein • Increase amount of protein in sample.present• Confirm protein is present through an alternateˇmethod.ˇ Gel ran too long • Decrease running time and monitor the gel byˇwatching dye front and ensuring the current isˇturned off when dye front is near bottom of gel.ˇ Old or degraded protein samples • Test a fresh sample of target protein.ˇ Protein over-transferred/ • Use a membrane with a smaller pore size.ˇ did not transfer correctly • Decrease voltage for lower MW proteins (lessˇthan 10kDa).ˇ• Make sure membrane is pre-soaked accordingˇto manufacturer’s instructions.ˇ• Check and optimize transfer time, asˇappropriate transfer time will vary with MW ofˇthe target protein.ˇ• Make sure transfer “sandwich” is set up correctlyˇand there is sufficient contact between gel andˇmembrane. (Please see our Western Transferˇprotocol for correct transfer “sandwich” set up.)ˇ• Ensure the correct transfer buffer is being used.ˇ• The presence of SDS during transfer can reduceˇthe binding of the protein when using aˇnitrocellulose membrane.ˇ• Add 20% methanol to transfer buffer to increaseˇbinding.ˇ• Check protein transfer by staining the membraneˇwith a reversible stain, such as Ponceau S, toˇvisualize major bands.ˇ Protein has a pI greater than 9 • Substitute buffer with a higher pH buffer (i.e.ˇ CAPS buffer, pH 10.5).ˇ Protein masked by blocking buffer • Test using a different blocking buffer.ˇ• Optimize concentration of protein in blockingˇbuffer. We recommend 3% Non-Fat Dry Milk inˇ diH 2O.ˇ Enzyme inhibitor present (i.e. azide) • Make sure buffers do not contain sodium azide,ˇas this will interfere with the HRP signal.ˇ Old or degraded primary antibody • Check manufacturer’s recommendations forˇstorage and/or expiration date. If expired or pastˇstorage time, purchase new antibody.ˇ• If antibody was subjected to repeated freeze/thawˇcycles purchase new antibody, as this can affectˇthe structure and protein:antibody binding.ˇ• Perform a Dot Blot to ensure antibody is still active.ˇ Insufficient amount of primary • Check manufacturer’s recommendation forˇ and/or secondary antibody antibody concentration.ˇ• Test a range of concentrations to find the optimalˇcondition for individual assay.ˇ Incorrect primary antibody • Ensure primary antibody reacts with the targetˇprotein from the species being studied (i.e.ˇ Rabbit-anti-human VEGF Human VEGF).INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se167

www.peprotech.com2011-2012 CatalogueWestern Transfer Troubleshooting Guide ContinuedINFORMATION & TECHNICAL SUPPORTProblem Possible source SuggestionWeak/No Signal Incorrect secondary antibody • Ensure the secondary antibody being used iscontinuedagainst the species of the primary antibody (i.e.ˇ Donkey-anti-rabbit IgG Rabbit-anti-human VEGF).ˇ Insufficient incubation • Increase incubation period of primary antibody.ˇ of primary antibody Antibody can be incubated for at least one hourˇat room temperature or overnight at 4°C.ˇ Insufficient incubation • Increase incubation period of secondary antibodyˇ of secondary antibody (please see manufacturer’s recommendations forˇincubation time and temperature).ˇ Membrane over-washed • Shorten wash times and/or reduce number of washes.ˇ• Use a washing buffer that does not contain aˇdetergent, or that has a lower concentration ofˇdetergent present.ˇ Incompatible developing reagent • Make sure the developing reagent being used isˇcompatible with the enzyme conjugate.ˇ• Ensure substrate is made correctly by followingˇthe manufacturer’s instructions.ˇ Excess salt present • Decrease amount of salt present in wash buffer.ˇ• Decrease amount of salt present in antibodyˇdilution buffer.ˇ Degraded/Incorrect developing • Ensure the developing reagent was preparedˇ reagent correctly.ˇ• Check that the reagent is still active. If not,ˇpurchase new reagent.ˇ For ECL method, film is bad/expired • Ensure film is not expired or exposed. If so,ˇpurchase new film.ˇ For ECL method, ECL developing • Use fresh ECL developing solutions.ˇsolutions are old/expiredˇ For ECL method, film exposure • Increase exposure time of the film.ˇtime is too shortˇ For ECL method, cling film • Use different brand of cling film.ˇinterfering with reactionHigh Background Antibody concentration too high • Check manufacturer’s recommendations forˇprimary and/or secondary antibody concentrations.ˇ• Test a range of concentrations to find optimalˇcondition for individual assay.ˇ Over-incubation of antibodies • Check manufacturer’s protocol to ensure theˇcorrect incubation time and temperature is used.ˇIf this information is not supplied by theˇmanufacturer, optimize incubation time throughˇtesting.ˇ Non-specific binding of antibodies • Ensure the primary antibody is specific only forˇthe protein that is being targeted.ˇ• Run a control, omitting the primary antibodyˇincubation. If bands develop, choose a differentˇsecondary antibody.ˇ• Choose a different host species for secondaryˇantibody.168To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comProblem Possible source SuggestionHigh Background Cross reaction of antibodies with • Use a different blocking buffer formulation.čontinued proteins in blocking buffer • Goat antibodies will react with BSA due to aˇ and/or sample slight recognition of the bovine protein by theˇgoat antibody. This will lead to a purple sheen onˇyour membrane. If this occurs, use a differentˇspecies antibody or a different blocking buffer.ˇ• If secondary antibody is suspected, diluteˇantibody in buffer containing 1-5% normal serumˇfrom the same species as target protein.ˇ Ineffective blocking buffer • Use a different blocking buffer formulation.ˇ• Add detergent to blocking buffer, such asˇTWEEN-20.ˇ Insufficient blocking • Use a different blocking buffer formulation.ˇ• Batch of blocking buffer may be inactive. Useˇfresh blocking buffer.ˇ• If blocking overnight at 4°C, this may decreaseˇblocking efficiency since lower temperatures mayˇlessen the effectiveness. Try incubation for oneˇhour at room temperature.ˇ• Add TWEEN-20 to blocking buffer.ˇ• Optimize incubation time and/or temperature ofˇblocking step.ˇ Insufficient washing • After each step, wash membrane 3 times for 10ˇminutes each. Increase the duration of theˇwashing step if this is not sufficient.ˇ• Try a different detergent in washing buffer or addˇTWEEN-20 if not already present.ˇ• Increase volume of washing buffer used.ˇ Over-incubation in color • Shorten incubation time in color developmentˇ development substrate solution. Monitor development until bands areˇpresent and stop reaction by washing membraneˇ in diH 2O.ˇ Excess enzyme present • Reduce concentration of selected enzyme conjugate.ˇ Interference by endogenous enzyme • Non Fat Dry Milk contains endogenous biotin andˇmay interfere with avidin/biotin detection system.ˇUse different blocking buffer, such as 3% BSA.ˇ Contaminated reagents • Filter buffers before use to remove contaminant.ˇ• Make fresh buffers and re-run Western Transfer.ˇ Membrane problems • Test new membranes.ˇ• During the entire procedure the membrane canˇonly be dried between transfer and immunostaining.ˇOnce immunostaining has begun, the membraneˇcannot be dried until completion of immunostainingˇprocedure. Re-run Western Transfer if membraneˇwas dried at any other point duringˇimmunostaining procedure.ˇ For ECL method, ECL film • Shorten film exposure time.ˇ overexposed • If signal from target protein is too strong, wait 5-ˇ10 minutes and re-expose to film.INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se169

www.peprotech.com2011-2012 CatalogueWestern Transfer Troubleshooting Guide ContinuedINFORMATION & TECHNICAL SUPPORTProblem Possible source SuggestionSplotchy background Uneven agitation during incubations • Ensure the membrane is agitated evenly byˇplacing on a rocker or shaker during incubation.ˇ Insufficient amount of incubation • Ensure enough solution is present during eachˇ buffer incubation period to fully submerge theˇmembrane and allow it to float freely in theˇsolution.ˇ Air bubbles present during transfer • Gently remove all air bubbles from transferˇ“sandwich” before transfer is started. This can beˇdone by using a roller or clean glass rod/Pasteurˇpipette.ˇ Membrane problems • Ensure membrane is wetted thoroughly accordingˇto the manufacturer’s protocol.ˇ• Handle membranes with extreme care.ˇMishandled membranes can cause non-specificˇbinding.ˇ• Handling membranes with bare hands can lead toˇcontamination of membrane. Use forceps or wearˇgloves when handling membrane.ˇ Aggregation of HRP conjugate • If using HRP, filter conjugate to remove HRPˇaggregates.ˇ• Use a fresh sample of HRP conjugate.ˇ Contamination of reagents • Filter buffers before use to remove contaminant.ˇ• Make fresh buffers and re-run Western Transfer.Non-specific bands Antibody concentration too high • Check manufacturer’s recommendations forˇprimary and/or secondary antibody concentrations.ˇ• Test a range of concentrations to find optimalˇcondition for individual assay.ˇ Old or degraded antibody • Check manufacturer’s recommendations forˇstorage and/or expiration date. If expired or pastˇstorage time, purchase new antibody.ˇ• If antibody was subjected to repeated freeze/thawˇcycles purchase new antibody, as this can affectˇthe structure and protein:antibody binding.ˇ Non-specific binding of primary • Decrease concentration of primary antibody.ˇ antibody • Check concentration of protein being added to gel.ˇ• Use a more specific antibody, such as aˇmonoclonal antibody or Antigen Affinity Purifiedˇpolyclonal antibody.ˇ• Add a small amount of detergent, such asˇTWEEN-20 (0.1-0.5%) to the primary antibodyˇsolution and/or blotting buffer.ˇ• Increase number/duration of washes.ˇ Non-specific binding of secondary • Decrease concentration of secondary antibody.ˇ antibody • Run a control, omitting the primary antibodyˇincubation. If bands develop, choose a differentˇsecondary antibody.ˇ• Add a small amount of detergent, such asˇTWEEN-20 (0.1-0.5%) to the secondary antibodyˇsolution.ˇ• Increase number/duration of washes.170To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comProblem Possible source SuggestionNon-Specific Bands Aggregation of target protein • Add or increase a reducing agent, such as DTT orcontinuedBME, to ensure all disulfide bonds are reduced.ˇ • Heat protein sample in a hot water bath for 5-10ˇminutes before loading onto gel.ˇ• Check concentration of protein being added toˇgel. A concentration that is too high can causeˇprotein aggregation. Re-run Western Transferˇwith a lower concentration of protein.ˇ Degradation of protein sample • Make fresh samples and minimize freeze/thawˇcycles of samples.ˇ• Add protease inhibitor to samples prior to storage.ˇ• Keep samples stored between -20°C and -80°C.ˇ Insufficient blocking • Test a different blocking buffer formulation.ˇ• Batch of blocking buffer may be inactive. Use aˇfresh blocking buffer.ˇ• If blocking overnight at 4°C, this may decreaseˇblocking efficiency since lower temperatures mayˇlessen the effectiveness. Try incubation for oneˇhour at room temperature.ˇ• Add TWEEN-20 to blocking buffer.ˇ• Optimize incubation time and/or temperature ofˇblocking step.ˇ SDS facilitates binding to • Ensure membrane is sufficiently washed afterˇ immobilized protein bands transfer of proteins.ˇ• Do not use SDS during immunostaining whenˇusing a nitrocellulose membrane.ˇ Excess enzyme present • Reduce concentration of the selected enzymeˇconjugate.ˇ Contamination of reagents • Filter buffers before use to remove contaminant.ˇ• Make fresh buffers and re-run Western Transfer.Diffused/Fuzzy bands Antibody concentration too high • Check manufacturer’s recommendations forˇprimary and/or secondary antibody concentrations.ˇ• Test a range of concentrations to find optimalˇcondition for individual assay.ˇ Excess protein on gel • Further dilute protein samples before loading onto gel.ˇ Gel over-heated/high voltage • Heat causes the gel to lose its structure andˇresolving power. Be sure the transfer reservoir isˇrun in an ice bath.ˇ• Reduce voltage or current used during transfer.ˇ Incorrect preparation of membrane • Pre-soak membrane in correct transfer solutionˇ(see membrane manufacturer’s protocol). Ensureˇit is soaked for the correct amount of time.White Bands Antibody or protein concentration • White bands are the result of rapid consumptionˇ(ECL visualization too high/Excessive signal generated of the substrate caused by the presence of tooˇmethod)much conjugate. This is the result of highˇconcentrations of the antibodies and/or proteins.ˇCheck manufacturer’s recommendations forˇantibody and/or protein concentrations.INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se171

www.peprotech.com2011-2012 CatalogueImmunohistochemistryINFORMATION & TECHNICAL SUPPORTThe analysis of tissues and cells for the presenceWorking with cells and tissue can pose difficultproblems due to the elaborate and complex natureof specific proteins is an important component of numerousclinical diagnostic and research programs. of these structures. In order to accurately examine theseImmunohistochemistry (histo=tissue) and immunocytochemistry(cyto=cell) are powerful techniques that uti- possible, the native state of the tissue being analyzed.complex samples it is vital to preserve, to the extentlize the specific interaction between a target protein and This is accomplished through a technique known asits corresponding antibody to visualize a protein of in- fixation. The fixation process is a cornerstone in immunohistochemistryand helps maintain the integrityterest in a complex biological sample. The terms immunohistochemistry(IHC) and immunocytochemistry and authenticity of the proteins and structures presentare often used interchangeably due to the intimate rela- in the samples. This is usually achieved by inhibitingtionship between cells and tissues. Two common de- the innate degradative processes present in the samplet e c t i o n m e t h o d s u s e d i n I H C a r e d i r e c t tissue by some combination of cryostasis, treatment withimmunoflouresence, in which the detecting antibody is chemical fixatives, and embedding tissue samples indirectly labeled with a fluorophore such as fluorescein paraffin. One of the most commonly used fixative comorrhodamine, and immunoenzymatic staining, in which binations is the formalin-fixed paraffin-embeddedthe detecting antibody is conjugated to a chromogen (FFPE) fixation method. To achieve maximum recognitionof the target protein, it is necessary to optimizeactivating enzyme either directly, or indirectly throughthe use of a conjugated secondary antibody.the fixation process for each sample type analyzed. Fac-Figure V-1 - Indirect Immunoenzymatic Detection Method172To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comtors which influence the optimization of the fixationprocess include the purity, affinity and specificity ofthe detecting antibody, the structure and stability of thetarget protein, and the structural and biochemical characteristicsof the tissue sample.The fixation process, while critical for maintainingthe integrity of the tissue, often masks the antibodyrecognition epitope on the target protein by inducingchemical modification, conformational alterations, orprotein aggregation. Therefore, most IHC procedurescontain an unmasking step, which is frequently called“antigen retrieval” or “epitope recovery”. This unmaskingprocess can be accomplished by several procedures,including HIER (Heat-Induced Epitope Retrieval) andPIER (Protease-Induced Epitope Retrieval), which,depending on the sample and the target protein, can beused alone or in tandem.The detection strategies commonly applied in IHCprocedures can be classified into two general categories,designated as direct and indirect detection methods.The direct detection methods utilize a labeled an-tibody, capable of fluorescing or activating a chemicalchromogen, which binds directly to the target protein.This method has the advantages of simplicity, low nonspecificbinding in most cases, and short assay “runtimes.” Indirect methods use an unlabeled primary antibodyto initially bind the target protein, which is thenrecognized by a labeled secondary antibody. Some indirectIHC detection procedures use a “three stage” procedure,in which an unlabeled secondary antibody isused and subsequently recognized by a labeled tertiaryantibody. Most indirect methods produce some degreeof signal amplification during the multiple stages of thedetection procedure, which consequently results in increasedassay sensitivity.Figure V-1 is a sample IHC detection protocolrepresenting the use of an “indirect immunoenzymatic”method.References(1) Boenisch, Thomas ed. Immunochemical staining procedureshandbook. 3 rd ed. Carpinteria, CA; 2001.(2) Janeway, et al. Immunobiology: the immune system in healthand disease. 6 th ed. New York: Garland Science, 2005.INFORMATION & TECHNICAL SUPPORTGoat anti-human VEGFHuman Breast Ductal Carcinoma immunochemically stainedwith Goat anti-human VEGF (Catalogue #500-P10G) using aHorseradish Peroxidase-DAB (brown) detection system withhematoxylin (blue) counterstain.Dark brown areas indicate stained carcinoma tumor cells.Light brown areas indicate stained duct and vessel lining.Rabbit anti-human IL-6Human Cervical Squamous Cell carcinomaimmunochemically stained with Rabbit anti-human IL-6(Catalogue #500-P26) using an Alkaline Phosphatasepolymer enhanced detection system with Permanent Red andhematoxylin (blue) counterstain.The cytoplasm of the carcinoma cells is stained red.Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se173

www.peprotech.com2011-2012 CatalogueImmunohistochemistry Troubleshooting GuideINFORMATION & TECHNICAL SUPPORTProblem Possible Source SuggestionWeak/No Staining Tissue Processing • The antigen may have been destroyed during the tissue processing.ˇ• Ensure tissue is prepared properly prior to immunostainingˇprocedure. Use proper fixative, fixation time, embedding reagentsˇand embedding techniques. Use proper sectioning techniquesˇand equipment. The recommended tissue thickness for IHC isˇ4µm sections. For formalin-fixed, paraffin-embedded tissue,ˇensure slides are completely deparaffinized and rehydrated.ˇReplace xylenes and alcohols as needed.ˇ Antigen Retrieval • Determine optimal antigen retrieval conditions. Consider stainingˇ (Epitope Retrieval) a tissue section without antigen retrieval.ˇ• Use HIER (Heat Induced Epitope Retrieval). Adjust and maintainˇthe temperature. Adjust the pH (choose appropriate buffer). Adjustˇthe amount of time.ˇ• Use EIER (Enzyme Induced Epitope Retrieval). Choose anˇappropriate enzyme (e.g. proteinase K). Adjust the incubationˇtime. Perform antigen retrieval at room temperature or with heat.ˇ• Do not reuse antigen retrieval solution. Perform adequate washˇsteps.ˇ Primary Antibody • Ensure antibodies are prepared as per data sheet:ˇ• Centrifuge vial prior to opening.ˇ• Once the antibody is reconstituted, centrifuge vial to remove anyˇpossible aggregates.ˇ• Aliquot and freeze antibody at -20˚C. Avoid repeated freeze/thawˇcycles.ˇ• Use antibody before expiration date.ˇ• Increase antibody concentration.ˇ• Increase incubation time. (e.g. stain antibody overnight at 4˚C.)ˇ Secondary Antibody • Match primary antibody with appropriate secondary reagent orˇdetection system.ˇ• Increase secondary antibody concentration or incubation time.ˇ Detection System • Use appropriate detection system.ˇ• Ensure enzyme and chromogen are compatible (e.g. DABˇchromogen requires the HRP enzyme).ˇ• Use reagents in proper order.ˇ• Perform appropriate wash steps.ˇ• Use components at room temperature.ˇ• Use components prior to expiration dates.ˇ• Use an amplified detection system, such as a polymer detectionˇsystem or tyramide amplification. A direct labeling system mayˇhave less amplification and may produce a weaker signal.ˇ• Sodium azide is an inhibitor of horseradish peroxidase and mayˇinterfere with staining results if present in certain reagents, suchˇas an HRP-conjugated secondary antibody. Limited amounts ofˇsodium azide in other reagents should not interfere with theˇimmunostaining procedure.174To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comˇProblem Possible Source Suggestionˇ Antigen • This antigen may not be present in the particular tissue or at aˇsignificant level for detection.ˇ• The epitopes of the antigen may have been destroyed duringˇtissue processing or antigen retrieval. Also, if using a monoclonalˇantibody and that particular epitope is masked or destroyed, thenˇthe antibody will not stain. Consider re-staining or reprocessing aˇnew tissue sample.ˇ• Use an alternative assay to detect for the presence of the antigenˇ(e.g. in situ hybridization).ˇ Chromogen • Adjust incubation time. Check for chromogen precipitate and doˇnot use past expiration date.ˇ• Use appropriate mounting media for each chromogen (e.g. DABˇcan be coverslipped with a permanent mounting media, while aˇnon-alcohol soluble AEC will require an aqueous mounting media).Over-staining Tissue Processing • Ensure tissue is prepared properly prior to immunostainingˇprocedure. Use proper fixative, fixation time, embedding reagentsˇand embedding techniques. Use proper sectioning techniquesˇand equipment. The recommended tissue thickness for IHC isˇ4µm sections. For formalin-fixed, paraffin-embedded tissue,ˇensure slides are completely deparaffinized and rehydrated.ˇReplace xylenes and alcohols as needed.ˇ Antigen Retrieval • The tissue may be over-retrieved. Adjust the incubation time.ˇAdjust the temperature. If necessary, use alternate antigenˇretrieval methods. Stain a tissue section without antigen retrieval.ˇ Primary Antibody • Ensure antibodies are prepared as per data sheet:ˇ• Centrifuge vial prior to opening.ˇ• Once the antibody is reconstituted, centrifuge vial to remove anyˇpossible aggregates.ˇ• Aliquot and freeze antibody at -20˚C. Avoid repeated freeze/thawˇcycles.ˇ• Use antibody before expiration date.ˇ• Decrease antibody concentration. Adjust antibody incubation time.ˇ• Incubate tissue specimen with a protein block just prior to theˇprimary antibody incubation (see protein block below).ˇ Secondary Antibody • Adjust concentration of secondary antibody. Use a secondaryˇantibody that is less likely to exhibit non-specific binding (e.g.ˇcross-adsorbed antibody or F(ab’) 2fragment).INFORMATION & TECHNICAL SUPPORTˇ Detection System • Use a detection system that will exhibit less non-specific binding.ˇ• Adjust incubation time(s).ˇ Non-specific Binding • Block endogenous molecules and highly charged molecules.ˇ Endogenous • Depending on the tissue type and staining procedure, endogenousˇ Molecules molecules may be present and interfere with staining. Determineˇthe necessary blocking steps for each tissue, such as blocking forˇendogenous molecules such as endogenous biotin, endogenousˇperoxidase, endogenous phosphatase, or Fc Receptors.ˇ• Choose an alternative staining method when an endogenousˇmolecule may be difficult to completely block (such as a non-ˇbiotin detection method on liver, which is rich in biotin).Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se175

www.peprotech.com2011-2012 CatalogueImmunohistochemistry Troubleshooting Guide Continued...Problem Possible Source Suggestionˇ Protein Block • Use a protein block or serum block from the host animal of theˇsecondary antibody to prevent non-specific binding (antibodiesˇare highly charged proteins and the molecules’ hydrophobicityˇmay cause non-specific staining).ˇ• Incubate the tissue specimen with the protein block prior to theˇprimary antibody incubation.INFORMATION & TECHNICAL SUPPORTˇ Inadequate washing • Increase the amount of time and/or the number of washes. Useˇthe appropriate buffer pH for different staining systems (e.g. PBSˇor Tris wash buffers). Add a detergent, such as TWEEN-20 toˇreduce hydrophobic interactions.ˇ Chromogen • Adjust incubation time. If possible, adjust the chromogenˇconcentration. Check for precipitate and do not use pastˇexpiration date.ˇ Tissue sections • Do not allow tissue sections to dry out during the immunostainingˇ dried out procedure. Use a humidity chamber and sufficient reagentˇvolume during tissue incubations. Use a PAP pen to create aˇhydrophobic barrier around the tissue section to ensure the tissueˇremains covered with a reagent.Other Conditions Reagent Expiration • Check the expiration dates of all reagents used during theˇ Dates immunostaining procedure.ˇ Controls • Ensure positive and negative controls are valid.ˇ Surfactant wash • Add a surfactant wash step to enhance reagent penetration (e.g.ˇ PBS/0.2% Triton X 100).ˇ Mounting Medium • Use appropriate media with each chromogen (differentˇchromogens require aqueous or non-aqueous mounting medias).ˇ Counterstain • Counterstain for the optimal amount of time.ˇ Automated Staining • Check machine to ensure proper specifications.176To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comMicroarrayMicroarray technology enables the simultaneousdetection and quantification of a large number ofproteins or nucleic acids in a variety of biologicalsamples, including cells, tissues, and bodily fluids. Theterm microarray refers to a miniaturized solid supportor chip, such as a nylon membrane or glass microscopeslide, which is spotted with a capture element (antibody,protein, oligonucleotide, cDNA, etc.) in a grid-like configuration.There are many applications of microarraysincluding assessing protein families, probing serum fordiagnostic information, profiling expression in responseto drugs, and profiling cells treated with certain stimulatorsor transfected with a given gene. Although thereare many procedural variations, the five essential stepsin the preparation of a microarray are: 1) capture elementsynthesis, 2) preparation of the solid support surface,3) immobilization of the capture elements ontothe solid support (using a robotic arrayer), 4) bindingof the target molecule to the immobilized capture elements,and 5) detection and quantification of the target/captureelement complex.Antibody microarrays use antibodies, raisedagainst known protein antigens, as the capture elementsand proteins capable of specifically binding to the immobilizedantibodies as the detected target molecules.Fluorescent dyes such as Cy3, Cy5, FITC, and TexasRed, or enzyme systems such as biotin-horseradish peroxidase,are a sensitive means of detection and visualization.Antibody microarrays exploit many of the sameprinciples applied to well established immunoassayprocedures such as ELISA or Western blot; however,microarrays offer many advantages including an increasednumber of data points (which has a direct effecton accuracy), the ability to detect many proteinssimultaneously, and reduced antibody and sample volumerequirements. In addition, once an array hasbeen designed and the components validated, a roboticarrayer can rapidly spot limitless copies for use withlarge sample groups.In this article, two antibody microarray formatswill be highlighted: direct labeling and fluorescence-linkedimmunosorbent assay (FLISA). Both assaysuse specific antibodies as the capture element. Indirect labeling, all proteins in the sample mixture arelabeled prior to capture in contrast to FLISA, wheretarget proteins from an untreated sample mixture arecaptured and subsequently detected through the use ofa biotinylated antibody and a fluorophore-labeled secondaryantibody.Antibodies used in microarray assays workbest when they are highly purified, exhibit minimalcross-reactivity, and have been shown to work well intraditional ELISA formats. Initially, antibody solutionsare prepared at a pre-determined optimal concentration(usually 300-500µg/ml), and then they are assembledinto a print plate or microtiter plate, which is used by arobotic arrayer to prepare a spotted solid support. Sixto ten replicates per antibody are usually sufficient and,depending on the researcher’s needs, up to 500 differentantibodies per array can be spotted and immobilizedfor comparison. The spotted support is blockedby treatment with a BSA solution for at least one hour,and then, the support is ready to be exposed to thesamples of interest (serum, urine, cell lysate, etc).Figure 1 depicts direct labeling detection. Allproteins in a complex mixture are conjugated to eithera fluorophore (Figure 1A) or biotin prior to incubationwith the capture element. The arrays are thenwashed and scanned for data analysis. The comparisonof signal intensities between samples can be usedto measure the relative amounts or expression levels ofdetected proteins. Alternatively, the comparison of signalintensities to calibrated protein standards, labeledwith distinct fluorphores, allows exact expression lev-INFORMATION & TECHNICAL SUPPORTBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se177

www.peprotech.com2011-2012 CatalogueMicroarray ContinuedINFORMATION & TECHNICAL SUPPORTels to be quantitatively measured (Figure 1B). Advantagesof this method are its simplicity and the requirementof only one specific antibody per target. The primarydisadvantage is increased background signal resultingfrom the labeling of all proteins in the samplemixture (e.g. albumin in serum). Detection sensitivityis limited by the concentration of the background proteinrelative to the target protein and is typically around100ng/ml in serum.In FLISA detection (Figure 2), antibodiesspotted on the microarray capture unlabeled proteintar-gets. This is followed by incubation with a cocktailof biotin-labeled antibodies, each antibody correspondingto one of the spotted capture antibodies. Finally, thearray is incubated with a suitable detection antibodysuch as a fluorophore labeled anti-biotin secondary antibody.The FLISA detection format results in increasedsensitivity, relative to the direct labeling method, becausebackground is significantly reduced. Sensitivity in thisformat can be enhanced even further using tyramide signalamplification or rolling-circle amplification (3).However, optimization of assays measuring many targetscan be difficult using this format; therefore, FLISAdetection is usually used for a limited number of targetsthat are present at concentrations below the detection limitof the direct labeling method. Although the detectionlimits for the assay depends on a number of parameters,including the affinity and specificity of the antibodiesused, the protein background in the sample, and the detectionconditions chosen, the FLISA method can providedetection limits in the low pg/ml range.References:(1) Fung, Eric ed. Protein arrays: methods and protocols. Fremont,CA: Humana Press Inc.; 2004.(2) Ye, S. and Day, I. eds. et al. Microarrays and Microplates:applications in biomedical sciences. Oxford, UK: BIOSScientific Publishers Ltd.; 2003.(3) Zhou, Heping, et al. Genome Biology, Vol. 5, issue 4, R28; 2004.178To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comFigure 1B - Multi-Color Detection Figure 2 - FLISA agINFORMATION & TECHNICAL SUPPORTg a Bestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se179

www.peprotech.com2011-2012 CatalogueIndexINDEX14 kDa lectin...................................................... see Galectin-126 kDa protein.............................................................see IL-628 kD (GBP28)................................................see Adiponectin4-1BB Ligand.................................................................... p.164-1BB Receptor................................................................. p.166Ckine..................................................................see Exodus-2ABCD-1.....................................................................see MDCABCD-2....................................................................see TARCAcrp30.............................................................see AdiponectinACT-2.................................................................... see MIP-1βActivin A............................................................................ p.12Activin B........................................................................... p.12activins-binding protein......................................see FollistatinAdipogenesis inhibitory factor (AGIF)..................... see IL-11Adipolean (human)............................................... see gAcrp30Adipolean variant (human).......................see gAcrp30 VariantAdiponectin....................................................................... p.12AdipoQ............................................................see AdiponectinAdipose tissue-specific secretory factor (ADSF).. see ResistinAIGF........................................................................ see FGF-8AITRL............................................................................... p.12Aldesleukin..................................................................see IL-2ALP .......................................................................see CTACKAlpha defensin-1........................................................see NP-1AMAC-1...................................................................see MIP-4Aminopeptidase ................................................................ p.13Amphiregulin..................................................................... p.13Angiopoietin-1 (ANG-1)................................................... p.13Apm-1 variant...........................................see gAcrp30 VariantApm-1..............................................................see AdiponectinApo I.............................................................. see Fas ReceptorApo I Ligand..................................................... see Fas LigandApo2 Ligand............................................................see TRAILApo3 Ligand.........................................................see TWEAKApoA-1.............................................................................. p.13ApoE2................................................................................ p.14ApoE3................................................................................ p.14ApoE4................................................................................ p.14Apo-SAA........................................................................... p.14AR .............................................................. see AmphiregulinAPRIL................................................................................ p.15APTL................................................................ see Fas LigandARIA..............................................................see Heregulinβ-1ART.......................................................................see ArteminArtemin.............................................................................. p.15ATAC............................................................see LymphotactinATF-1....................................................................... see GDNFB71.................................................................................... p.16B cell stimulatory factor-2 receptor.....................IL-6 receptorBAFF................................................................................. p.17BAFF Receptor.................................................................. p.17BCA-1................................................................................ p.18BCDF...........................................................................see IL-6BCDFII........................................................................see IL-5BCGF...........................................................................see IL-4BCM........................................................................see BCMABCMA................................................................................ p.18BD-1.................................................................................. p.19BD-2.................................................................................. p.19BD-3.................................................................................. p.19BD-4.................................................................................. p.19BDNF................................................................................ p.20Betacellulin........................................................................ p.20Beta-glactoside-binding lectin L-14-I............... see Galectin-1BLC.........................................................................see BCA-1BLR1 Ligand...........................................................see BCA-1BLys.......................................................................... see BAFFBMP-2...............................................................................p.21BMP-3...............................................................................p.21BMP-4...............................................................................p.21BMP-6...............................................................................p.22BMP-7...............................................................................p.22BMP-11.................................................................see GDF-11BMP-13............................................................................. p.22BMP-14...................................................................see GDF-5BMP-15................................................................ see GDF-9BBolekine...................................................................see BRAKB-R1.........................................................................see I-TACBRAK................................................................................ p.23Breast cancer cell differentiation factor p45.............. see Heregulinβ-1BSC-1.................................................................... see TGF-β2BSF-1 (B-cell stimulating factor)................................see IL-4BSF-2...........................................................................see IL-6BTC.................................................................see BetacellulinB-TCGF.....................................................................see IL-10C10.................................................................................... p.23Cachectin.................................................................see TNF-αCarboxypeptidase B........................................................... p.23Cardiotrophin-1................................................................. p.24Cardiotrophin-like cytokine......................... see NNT-1/BSF-3Cartilage-inducing factor....................................... see TGF-β1Catabolin....................................................................see IL-1βCCF18.....................................................................see MIP-1γCCK-1........................................................................ see MECCCL1......................................................................... see I-309CCL2...................................................................... see MCP-1180To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comCCL3...................................................................... seeMIP-1αCCL3L1...................................................................see LD78βCCL4..................................................................... see MIP-1βCCL4L1...................................................................see LAG-1CCL5...................................................................see RANTESCCL6........................................................................... see C10CCL7...................................................................... see MCP-3CCL8...................................................................... see MCP-2CCL9/10.................................................................see MIP-1γCCL11....................................................................see EotaxinCCL12.................................................................... see MCP-5CCL13.................................................................... see MCP-4CCL14.....................................................................see HCC-1CCL15......................................................................see MIP-5CCL16.........................................................................see LECCCL17......................................................................see TARCCCL18......................................................................see MIP-4CCL19................................................................... see MIP-3βCCL20.................................................................... seeMIP-3αCCL21.................................................................see Exodus-2CCL22.......................................................................see MDCCCL23......................................................................see MIP-3CCL24................................................................ see Eotaxin-2CCL25......................................................................see TECKCCL26................................................................ see Eotaxin-3CCL27...................................................................see CTACKCCL28....................................................................... see MECCCN2........................................................................see CTGFCCN4..................................................................... see WISP-1CCN5......................................................................see CTGFLCCN6..................................................................... see WISP-3sCD14................................................................................p.24sCD22................................................................................ p.24sCD23................................................................................ p.25sCD27L.............................................................................. p.25sCD30L.............................................................................. p.25sCD100.............................................................................. p.26CD120a.............................................see TNF Receptor Type ICD120b............................................see TNF Receptor Type IICD124...........................................................see IL-4 ReceptorCD126...........................................................see IL-6 ReceptorCD137 antigen..........................................see 4-1BB ReceptorCD137L....................................................... see 4-1BB LigandCD154........................................................... see CD40 LigandCD25 antigen................................................see IL-2 ReceptorCD252 antigen...................................................... see sOX40LCD40 Ligand..................................................................... p.26CD95.............................................................. see Fas ReceptorCD95L.............................................................. see Fas LigandCDMP-1..................................................................see GDF-5CDMP-2............................................................... see BMP-13CDNF................................................................................ p.27Cetermin................................................................ see TGF-β2Chemerin........................................................................... p.27CINC........................................................................ see GROαCkB-6................................................................. see Eotaxin-2c-Kit Ligand.................................................................see SCFCkβ-8........................................................................see MIP-3c-MPL Ligand..............................................................see TPOCNTF................................................................................. p.27Colon Cancer Cell Growth Inhibitor................... see IGF-BP4Control Goat Immunoglobulin........................................ p.141Control Mouse Immunoglobulin..................................... p.141Control Rabbit Immunoglobulin..................................... p.141CPB-35.............................................................. see Galectin-3Crg-2.......................................................................... see IP-10Cryptdin......................................................................see NP-1CSF-1...................................................................... see M-CSFCSF-2................................................................... see GM-CSFCSF-3.......................................................................see G-CSFCSIF...........................................................................see IL-10CT-1.......................................................... see Cardiotrophin-1CT-58......................................................................see CTGFLCTACK ............................................................................. p.28CTAP-III..................................................................see NAP-2CTGF................................................................................. p.28CTGFL/WISP-2................................................................ p.28CTL maturation factor (TCMF)................................see IL-12CTLA-4............................................................................. p.29CTLA-8.................................................................. see IL-17ACX3CL1........................................................... see FractalkineCXCL1.................................................................... see GROαCXCL2.................................................................... see GROβCXCL3.....................................................................see GROγCXCL4....................................................................... see PF-4CXCL5..................................................................see ENA-78CXCL6....................................................................see GCP-2CXCL7....................................................................see NAP-2CXCL8........................................................................see IL-8CXCL9....................................................................... see MIGCXCL10.................................................................... see IP-10CXCL11....................................................................see I-TACCXCL12.............................................................see SDF-1α/βCXCL13..................................................................see BCA-1CXCL14..................................................................see BRAKCXCL16............................................................................ p.29INDEXBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se181

www.peprotech.com2011-2012 CatalogueIndexINDEXCYR61............................................................................... p.29Cysteine-rich secreted protein FIZZ1................... see RELMαCysteine-rich secreted protein FIZZ2....................see RELMβCytotoxic lymphocyte maturation factor (CLMF)....see IL-12Cytotoxin.................................................................see TNF-αDC-CK1....................................................................see MIP-4DDAH................................................................................ p.30DIF...........................................................................see TNF-αDifferentiation inhibiting factor............................. see TGF-β1Diphtheria toxin receptor......................................see HB-EGFDKK-1...............................................................................p.30DLL-1................................................................................p.30DLL-4................................................................................p.30DR3LG.................................................................see TWEAKDR4........................................................ see TRAIL receptor 1DR5........................................................ see TRAIL receptor 2DT-R.....................................................................see HB-EGFECGF-beta........................................................see FGF-acidicEDF..............................................................................see IL-5EGF.................................................................................... p.31EGF receptor..................................................................... p.32EG-VEGF.......................................................................... p.32ELC........................................................................ see MIP-3βEMAP-II............................................................................ p.32ENA-78.............................................................................. p.33Endostatin.......................................................................... p.33Enterokinase...................................................................... p.34Eotaxin............................................................................... p.34Eotaxin-2........................................................................... p.35Eotaxin-3........................................................................... p.35EP........................................................................... see IL-1α/βEPC-1....................................................................... see PEDFEPG..........................................................................see EpigenEpigen................................................................................ p.36Epiregulin.......................................................................... p.36Epithelial mitogen...................................................see EpigenEREG................................................................. see EpiregulinE-Selectin.......................................................................... p.36Eskine....................................................................see CTACKETGF.......................................................................see TGF-αExodus-1................................................................ see MIP-3αExodus-2............................................................................ p.37Exodus-3................................................................ see MIP-3βsFas Antigen.................................................. see Fas ReceptorFas Ligand......................................................................... p.38Fas Receptor...................................................................... p.38FGF-1...............................................................see FGF-acidicFGF-2................................................................ see FGF-basicFGF-4...........................................................................p.39-41FGF-5...........................................................................p.39-41FGF-6...........................................................................p.39-41FGF-7/KGF..................................................................p.39-41FGF-8...........................................................................p.39-41FGF-9...........................................................................p.39-41FGF-10.........................................................................p.39-41FGF-16.........................................................................p.39-41FGF-17.........................................................................p.39-41FGF-18.........................................................................p.39-41FGF-19.........................................................................p.39-41FGF-20.........................................................................p.39-41FGF-21.........................................................................p.39-41FGF-22.........................................................................p.39-41FGFA..................................................................... see FGF-10FGF-acidic....................................................................p.39-41FGF-basic.....................................................................p.39-41FGFG..................................................................... see FGF-16FGFH..................................................................... see FGF-17FGFI...................................................................... see FGF-18FGF-inducible 14.................................. see TWEAK ReceptorFGFJ...................................................................... see FGF-19FGFK..................................................................... see FGF-20FIZZ3..................................................................... see Resistinflt3L.................................................................. see flt3-Ligandflt3-Ligand......................................................................... p.42Folliculostellate cell-derived growth factor..............see VEGFFollistatin........................................................................... p.42Fractalkine......................................................................... p.43Frizzled related protein-1....................................... see sFRP-1FRP-1...................................................................... see sFRP-1FS........................................................................see FollistatingAcrp30............................................................................. p.43gAcrp30 variant................................................................. p.43GAF......................................................................... see FGF-9Galactose-specific lectin-3................................. see Galectin-3Galaptin............................................................. see Galectin-1Galectin-1.......................................................................... p.44Galectin-3.......................................................................... p.44GCP-2............................................................................... .p.45G-CSF................................................................................ p.46GDF-2................................................................................ p.46GDF-3............................................................................... .p.47GDF-5................................................................................ p.47GDF-6................................................................... see BMP-13GDF-8.................................................................see MyostatinGDF-11.............................................................................. p.47GDF-15/MIC-1.................................................................. p.47182To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comGDGF..................................................................... see PDGFsGDNF............................................................................... .p.48gelatin-binding protein....................................see AdiponectinGITRL.....................................................................see AITRLGlioblastoma-derived T cell suppressor factor...... see TGF-β2Glioma-derived endothelial cell mitogen.................see VEGFGLP-1................................................................................ p.48Glial Maturation Factor-beta..................................see GMF-βGlu-C................................................................................. p.48Glucagon-like peptide............................................. see GLP-1Glycoprotein Gp34............................................... see sOX40LGM-CSF............................................................................ p.49GM-CSFβ................................................................see G-CSFGMF-β............................................................................... p.50gp39.............................................................. see CD40 LigandGranzyme B....................................................................... p.50GROα................................................................................ p.51GROβ............................................................................... .p.51GROγ............................................................................... ..p.51GRO1....................................................................... see GROαGRO2....................................................................... see GROβGRO3........................................................................see GROγGrowth-hormone-dependent binding proteins.... see IGF-BP3H3 relaxin........................................................... see Relaxin-3HARP.............................................................. see PleiotrophinHB-EGF............................................................................. p.52HBGF-1............................................................ see FGF acidicHBGF-2..............................................................see FGF basicHBGF-4................................................................... see FGF-4HBGF-5................................................................... see FGF-5HBGF-6................................................................... see FGF-6HBGF-7.......................................................... see FGF-7/KGFHBGF-8................................................................... see FGF-8HBGF-9................................................................... see FGF-9HC14....................................................................... see MCP-2HCC-1................................................................................ p.52HCC-2.......................................................................see MIP-5HCC-3......................................................................see HCC-1HCC-4..........................................................................see LECHCGF (Hematopoietic cell growth factor)..................see IL-3HCS24......................................................................see CTGFHematopoietin-1....................................................... see IL-1αHeparin-binding growth factor-8 (HBGF-8).. see PleiotrophinHeregulinβ-1...................................................................... p.53HGF................................................................................... p.53HGNF.........................................................................see NT-3HHG-1......................................................see Sonic HedgehogHNP-1.........................................................................see NP-1HP1.A.......................................................................see TTF-1HPGF...........................................................................see IL-6HPTA.......................................................................... see HGFHRG...............................................................see Heregulinβ-1HSF..............................................................................see IL-6HST-1....................................................................... see FGF-4HST-2....................................................................... see FGF-6HVEM-L................................................................. see LIGHTHVEM-Fc.......................................................................... p.54I-309.................................................................................. p.54IBP-1.................................................................... see IGF-BP1IBP-2.................................................................... see IGF-BP2ICAM-1.............................................................................p.55IFN-b.................................................................................p.55IFN-β2.........................................................................see IL-6IFN-γ.............................................................................p.56-57IFN-λ1............................................................................... p.57IFN-λ2............................................................................... p.57IFN-w................................................................................. p.57IgE-binding protein........................................... see Galectin-3IGF-I.................................................................................. p.58IGF-IA.......................................................................see IGF-IIGF-II................................................................................. p.58IGF-BP1............................................................................ p.59IGF-BP2............................................................................ p.59IGF-BP3............................................................................ p.59IGF-BP4............................................................................ p.59IGF-BP5............................................................................ p.59IGF-BP6............................................................................ p.59IGF-BP7............................................................................ p.59IGFBPrP1............................................................ see IGF-BP7IL-10-inducible chemokine.........................................see LECIL-1α................................................................................. p.60IL-1β.................................................................................. p.61IL-1RA............................................................................... p.62IL-2.................................................................................... p.63IL-2 Receptor α................................................................. p.64IL-3.................................................................................... p.64IL-4.................................................................................... p.65IL-4 Receptor α................................................................. p.66IL-5.................................................................................... p.66IL-6...............................................................................p.67-68IL-6 Receptor α................................................................. p.68IL-7.................................................................................... p.69IL-8...............................................................................p.69-70IL-9.................................................................................... p.70IL-10.................................................................................. p.71IL-11.................................................................................. p.72INDEXBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se183

www.peprotech.com2011-2012 CatalogueIndexINDEXIL-12.................................................................................. p.73IL-13.................................................................................. p.74IL-13 variant...................................................................... p.74IL-15.................................................................................. p.75IL-16.................................................................................. p.75IL-17A/IL-17..................................................................... p.76IL-17B............................................................................... p.77IL-17D............................................................................... p.77IL-17E................................................................................ p.77IL-17F................................................................................ p.78IL-18BP............................................................................. p.78IL-19.................................................................................. p.79IL-20.................................................................................. p.79IL-21.................................................................................. p.80IL-22.................................................................................. p.81IL-23.................................................................................. p.82IL-25...........................................................................see SF20IL-28A.................................................................... see IFN-λ2IL-29....................................................................... see IFN-λ1IL-31.................................................................................. p.82IL-33.................................................................................. p.83IL-34.................................................................................. p.83IL-T............................................................................see IL-15IL-TIF........................................................................see IL-22Immune Interferon.....................................................see IFN-γInsulin-like peptide-7.......................................... see Relaxin-3INSL7................................................................. see Relaxin-3Int-1........................................................................ see WNT-1Int-2...........................................................................see FGF3IP-10.................................................................................. p.84Iroplact........................................................................ see PF-4I-TAC................................................................................. p.85JE............................................................................ see MCP-1KC............................................................................ see GROαKex-2................................................................................. p.85KGF......................................................................... see FGF-7KILLER................................................see TRAIL Receptor-2KLF4-TAT......................................................................... p.86Klotho................................................................................p.86L-29................................................................... see Galectin-3LAF........................................................................ see IL-1α/βLAG-1................................................................................ p.86LARC.................................................................... see MIP-3αLCC-1..........................................................................see LECLD78α................................................................... see MIP-1αLD78β................................................................................ p.86LEC.................................................................................... p.87LEM....................................................................... see IL-1α/βLeptin............................................................................p.87-88LIGHT............................................................................... p.88Lin28-TAT......................................................................... p.88LIX........................................................................see ENA-78Lkn-1........................................................................see MIP-5LMC............................................................................see LECLungkine............................................................................ p.89Luterotropic hormone...........................................see ProlactinLutetropin.............................................................see ProlactinLymphocyte chemoattractant factor (LCF)...............see IL-16Lymphopoietin-1 (LP-1)..............................................see IL-7Lymphotactin..................................................................... p.89Lymphotoxin-alpha (LT-α)...................................... see TNF-βMAC2................................................................ see Galectin-3MAF..........................................................................see IFN-γMammotropin.......................................................see ProlactinMARC.................................................................... see MCP-3Maspin............................................................................... p.89Mast cell growth-enhancing activity (murine)............see IL-9MCF....................................................................... see IL-1α/βMCGF..........................................................................see IL-3MCP-1..........................................................................p.91-92MCP-2..........................................................................p.91-92MCP-3 .........................................................................p.91-92MCP-4 .........................................................................p.91-92MCP-5..........................................................................p.91-92M-CSF............................................................................... p.90MDC.................................................................................. p.93MDNCF.......................................................................see IL-8MEC.................................................................................. p.93Megakaryocyted colony-stimulating factor.................see TPOMelanoma differentiation associated-like protein.....see IL-19MGDF..........................................................................see TPOMGF............................................................................see SCFMGI-1G...................................................................see G-CSFMGI-1GM............................................................ see GM-CSFMGI-2..........................................................................see IL-6MGI-IM.................................................................. see M-CSFMGSAα................................................................... see GROαMGSAβ................................................................... see GROβMGSAγ.....................................................................see GROγMIA.................................................................................... p.94MIA-2................................................................................ p.94MIAL....................................................................... see OTORMidkine.............................................................................. p.95MIG................................................................................... p.95MIP-1α.........................................................................p.96-97MIP-1β.........................................................................p.96-97184To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comMIP-1γ............................................................................... p.97MIP-2..........................................................................see GROMIP-3............................................................................... ..p.98MIP-3α.............................................................................. p.98MIP-3β.............................................................................. p.99MIP-4................................................................................. p.99MIP-5............................................................................... p.100MMP-1............................................................................ p.100MMP-2............................................................................ p.100MMP-3............................................................................ p.100Monocyte Arg-Serpin................................................see PAI-2MPIF-1.....................................................................see MIP-3MPIF-2............................................................... see Eotaxin-2MRP-1......................................................................... see C10MRP-2....................................................................see MIP-1γMTN-1.........................................................................see LECMulti-Colony stimulating factor .................................see IL-3Mycoplasma Arginine Deiminase .................................. p.100Myostatin......................................................................... p.101Myostatin Propeptide....................................................... p.101NAF.............................................................................see IL-8Nanog.............................................................................. p.101Nanog-TAT...................................................................... p.101NAP-1..........................................................................see IL-8NAP-2.............................................................................. p.102NAP-3...................................................................... see GROαNC30 (Human)..........................................................see IL-13NCC-1..................................................................... see MCP-4NCC-2......................................................................see HCC-1NCC-4..........................................................................see LECNecrosin...................................................................see TNF-αNEGF-2.................................................................see MidkineNeu differentiation factor..............................see Heregulinβ-1Neuregulin1...................................................see Heregulinβ-1Neuritin............................................................................p.102Neuropoietin.................................................................... p.102Neuroserpin..................................................................... p.103Neurotactin....................................................... see FractalkineNeurotrophic factor....................................................see NT-3Neurturin.......................................................................... p.103NGF-β.............................................................................. p.104NGF-2.........................................................................see NT-3NKSF.........................................................................see IL-12NNT-1/BSF-3.................................................................. p.105NOGGIN......................................................................... p.105NOV................................................................................. p.105NP-1................................................................................. p.106NP..................................................................see NeuropoietinNPO...............................................................see NeuropoietinNRG...............................................................see Heregulinβ-1NRTN..................................................................see NeurturinNT-3................................................................................. p.106NT-4................................................................................. p.106NT-4/NT-5..................................................................see NT-4NTN.....................................................................see NeurturinObesity protein (OB)................................................see LeptinOCIF......................................................... see OsteoprotegerinOct4-TAT ........................................................................ p.107ODAR...................................................... see RANK ReceptorODF...................................................................... see RANKLODFR...................................................... see RANK ReceptorODGF..................................................................... see PDGFsOncostatin A............................................................... see PF-4Oncostatin M................................................................... p.107OP-7........................................................................ see BMP-7OPG.......................................................... see OsteoprotegerinOPGL.................................................................... see RANKLOSF-1............................................................. see PleiotrophinOSM............................................................. see Oncostatin MOsteopontin...................................................................... p.107Osteoprotegerin............................................................... p.108OTOR.............................................................................. p.108Otoraplin.................................................................. see OTORsOX40L........................................................................... p.108OX40 Ligand (OX40L)........................................ see sOX40Lp16-INK4a.......................................................................p.109p16-INK4a-TAT...............................................................p.109P40 cytokine................................................................see IL-9P55...................................................see TNF Receptor Type IIP75...................................................see TNF Receptor Type IIP80...................................................see TNF Receptor Type IIP600 (Murine)...........................................................see IL-13PAF-AH...........................................................................p.109PAI-1................................................................................ p.109PAI-2................................................................................ p.109PARC........................................................................see MIP-4PBEF.......................................................................see VisfatinPBP..........................................................................see NAP-2PBSF...................................................................see SDF-1α/βP-cell stimulation factor..............................................see IL-3PDGF-AA........................................................................ p.110PDGF-AB....................................................................... .p.110PDGF-BB....................................................................... .p.110PDGF-CC ....................................................................... p.110PECAM-1.........................................................................p.111PEDF................................................................................p.111INDEXBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se185

www.peprotech.com2011-2012 CatalogueIndexINDEXPersephin..........................................................................p.111PF-4..................................................................................p.111PGF ............................................................................see PlGFPK2.............................................................. see Prokineticin-2Placenta Protein 12 (PP12).................................. see IGF-BP1Placental TGF-β....................................................see GDF-15Pleiotrophin..................................................................... p.112PlGF-1............................................................................. p.112PlGF-2............................................................................. p.113PlGF-3.............................................................................p.113Pluripoietin..............................................................see G-CSFPluripoietin-α...................................................... see GM-CSFPNR2........................................................................see TFF-1Polyergin................................................................ see TGF-β2Pre-B cell factor...........................................................see IL-7Pre-B cell colony-enhancing factor........................see VisfatinPROK1..............................................................see EG-VEGFProkineticin-1....................................................see EG-VEGFProkineticin-2.................................................................. p.113Prolactin .......................................................................... p.114Prostatropin........................................................ see FGF-basicProstate differentiation factor....................see GDF-15/MIC-1Protease inhibitor 5................................................. see MaspinProtease inhibitor 12....................................... see NeuroserpinProtein Bv8 homolog .................................. see Prokineticin-2pS2............................................................................see TFF-1PSP...................................................................... see PersephinPSPN................................................................... see PersephinPTHrP.............................................................................. p.114PTN..................................................................see pleiotrophinRANK receptor................................................................ p.116RANKL........................................................................... p.115RANTES.......................................................................... p.117Relaxin-2......................................................................... p.118Relaxin-3......................................................................... p.118RELMα........................................................................... p.118RELMβ............................................................................ p.119RELMγ............................................................................ p.119Resistin............................................................................ p.120Sarcoma growth factor............................................see TGF-αScatter Factor.............................................................. see HGFSCF.................................................................................. p.121SCGFα............................................................................. p.122SCGFβ............................................................................. p.122SCM-1a.........................................................see LymphotactinSDF-1α............................................................................ p.122SDF-1β............................................................................ p.122Serpin B2...................................................................see PAI-2Serpin B5................................................................ see MaspinSerpin E1...................................................................see PAI-1Serpin F1.................................................................. see PEDFSerpin I1......................................................... see NeuroserpinSF................................................................................ see HGFSF20................................................................................. p.123sFRP-1.............................................................................. p.43SIS-delta..............................................................see RANTESsHH...........................................................see Sonic HedgehogSkinkine.................................................................see CTACKS-LAC lectin-1.................................................. see Galectin-1SLC......................................................................see Exodus-2Slit2-N............................................................................. p.123Smag-82................................................................... see FGF-5Somatamedin A........................................................ see IGF-IISomatamedin C.........................................................see IGF-ISonic Hedgehog .............................................................. p.123Sox2................................................................................. p.123Sox2-TAT......................................................................... p.123SPARC/Osteonectin......................................................... p.124Spasmmolysin............................................................ see TFF2Spasmolytic Polypeptide (SP)................................... see TFF2SRPSOX.............................................................. see CXCL16STCP-1......................................................................see MDCSteel Factor..................................................................see SCFT cell growth factor p40..............................................see IL-9T cell interferon.........................................................see IFN-γTAC-antigen.................................................see IL-2 ReceptorTACI................................................................................ p.124TAFA............................................................................... p.125TALL-1..................................................................... see BAFFTARC............................................................................... p.125T-BAM.......................................................... see CD40 LigandTCA-3........................................................................ see I-309TCGF...........................................................................see IL-2TECK............................................................................... p.125TFF1................................................................................ p.126TFF2................................................................................ p.126TFF3................................................................................ p.126TGF-type I...............................................................see TGF-αTGF-α.............................................................................. p.126TGF-β1............................................................................ p.127TGF-β2............................................................................ p.127TGF-β3............................................................................ p.127TGIF..........................................................................see IL-10THANK.................................................................... see BAFFThrombomodulin ............................................................ p.127TIGAR ............................................................................ p.127186To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz

PEPROTECH ®www.peprotech.comTIGAR-TAT .................................................................... p.127TIMP-1............................................................................ p.128TIMP-2............................................................................ p.128TL-1A.............................................................................. p.129TL2..........................................................................see TRAILTL6..........................................................................see AITRLTLR-3..............................................................................p.129TNF Receptor Type I....................................................... p.131TNF Receptor Type II...................................................... p.132TNFAR............................................ see TNF Receptor Type ITNF-R55...........................................see TNF Receptor Type ITNFR60............................................see TNF Receptor Type ITNFR75.......................................... see TNF Receptor Type IITNFR80.......................................... see TNF Receptor Type IITNFRSF1A.......................................see TNF Receptor Type ITNFRSF1B......................................see TNF Receptor Type IITNFRSF6.....................................................see FAS ReceptorTNFRSF9.................................................see 4-1BB ReceptorTNFRSF10A........................................ see TRAIL Receptor-1TNFRSF10B........................................ see TRAIL Receptor-2TNFRSF11A............................................ see RANK ReceptorTNFRSF11B............................................. see OsteoprotegerinTNFRSF12A......................................... see TWEAK ReceptorTNFRSF13B.............................................................. see TACITNFRSF13C..............................................see BAFF ReceptorTNFRSF17..............................................................see BCMATNFSF1................................................................... see TNF-βTNFSF2...................................................................see TNF-αTNFSF4................................................................ see sOX40LTNFSF5........................................................ see CD40 LigandTNFSF6............................................................ see Fas LigandTNFSF7.................................................................see sCD27LTNFSF8.................................................................see sCD30LTNFSF9....................................................... see 4-1BB LigandTNFSF10.................................................................see TRAILTNFSF11.............................................................. see RANKLTNFSF12..............................................................see TWEAKTNFSF13.................................................................see APRILTNFSF13B............................................................... see BAFFTNFSF14................................................................ see LIGHTTNFSF15................................................................. see TL-1ATNFSF18.................................................................see AITRLTNF-α.............................................................................. p.130TNF-β............................................................................. .p.131TPO.................................................................................. p.132TR1........................................................... see OsteoprotegerinTRAIL............................................................................. p.133TRAIL receptor-1............................................................ p.133TRAIL receptor-2............................................................ p.134TRANCE.............................................................. see RANKLTRANCE receptor................................... see RANK ReceptorTransforming protein KS3....................................... see FGF-4TRAP............................................................ see CD40 LigandTRDL-1α.................................................................see APRILTRF..............................................................................see IL-5TRICK2A.............................................see TRAIL Receptor-2TRICKB.............................................. see TRAIL Receptor-2TSG.................................................................................. p.134TSLP................................................................................ p.134TSF............................................................................see IL-12TWEAK........................................................................... p.135TWEAK Receptor........................................................... p.135TXGP1.................................................................. see sOX40LType II interferon.......................................................see IFN-γUNQ222/PRO248....................................................see GDF-3URG.............................................................................see EGFUrogastrone.................................................................see EGFUrokinase inhibitor....................................................see PAI-2Vaspin.............................................................................. p.136VEGF-A....................................................................p.137-138VEGF-B........................................................................... p.138VEGF-C...........................................................................p.138VEGF-D..........................................................................p.139VEGI........................................................................ see TL-1AVisfatin............................................................................ p.139vMIP-2............................................................................... p.97VPF...........................................................................see VEGFWISP-1............................................................................ p.139WISP-2...................................................................see CTGFLWISP-3............................................................................ p.139WNT-1............................................................................. p.140WNT-3A.......................................................................... p.140WNT-7A.......................................................................... p.141Wnt-1-induced secreted protein............................ see WISP-1XCL-1.........................................................see LymophotactinzFGF5.................................................................... see FGF-18zTNF4....................................................................... see BAFFINDEXBestellungen: Deutschland: Tel: 0800 436 99 10 • email: info@peprotech.dePour commander: France: Tel: +33 (0)1 46 24 58 20 • email: info@peprotechfr.comTo Order: NORDIC: Tel: +46 (0)8 640 41 07 • email: info@peprotech.se187

www.peprotech.com2011-2012 CatalogueNotesINDEX188To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz