Standard PCR – Product Selection Guide - Jena Bioscience

14
Oligonucleotides
Standard Oligos
Custom DNA Primers from Jena Bioscience are synthetic oligonucleotides made to order with your specifi ed sequence.
They are well suitable for use in a variety of molecular biology or analytical/diagnostic applications ranging from simple
PCR and sequencing to probes for quantitative gene detection.
Scale [µmol]
Standard Purifi cation* OPC Purifi cation* HPLC Purifi cation
€ per base Yield [OD260] € per base Yield [OD260] € per base Yield [OD260]
0.02** 0.39 3 0.75 1 0.85 1
0.04 0.46 5 0.80 2.5 0.98 2.5
0.2 1.00 16 1.20 8 1.50 8
1.0 2.40 80 -- -- 3.00 25
10 -- -- -- -- 20.00 --
* Standard and OPC purifi cation can only be ordered for oligos 80 bases.
Amount of DNA
A yield of 1 OD 260 represents approximately 33 µg
of single-stranded DNA with an equal number of the
four bases. This corresponds to approximately 5 nmol
(50 µl / 100 µM) of a 20-mer oligonucleotide.
Storage
Avoid repetitive freeze/thaw cycles and long term storage
at concentrations below 20 µM. Aliquot oligonucleotides
before freezing.
Delivery Mode Storage Temperature Shelf Life
Lyophilized -20˚C 1 year
Lyophilized Room temperature 2 months
Solution -20˚C 6 months
Solution Room temperature 1 week
Synthesis Report
A comprehensive Synthesis Report comes along with
every oligo, indicating its name and sequence, synthesis
scale and yield (OD, µg, nmol), delivery mode (lyophilized
or solution), molecular weight, melting temperature,
GC-content, purifi cation mode and quality control.