Standard PCR – Product Selection Guide - Jena Bioscience

20
DNA Mutagenesis
The unique Jena Bioscience product series for random
mutagenesis provides you with “ready-to-go” kits for
inserting random mutations into your gene of interest.
All materials are accompanied by a streamlined documentation
that maximizes success.
Within three billion years of evolution, nature has produced
a plethora of proteins simply by repeated cycles
of random mutagenesis followed by in vivo selection for
superior function of the encoded proteins. This example
of natural evolution has guided researchers within the
last two decades to develop strategies for in vitro permutation
of proteins.
Among the variety of strategies applied, three major
powerful techniques have emerged.
Mutagenesis by dNTP Analogs
The method can achieve rates of mutagenesis of up
to 20 %. It is based on incorporation of mutagenic
dNTP analogs (8oxo-dGTP
and dPTP)
into an amplifi ed
DNA fragment by a
standard PCR. The
mutagenic dNTPs are
eliminated by a second
PCR step in the
presence of the four
natural dNTPs, leaving
highly mutated
Rate of mutagenesis as a function of the
number of PCR cycles
DNA ready for further
investigation.
Mutagenesis by Error-Prone PCR
Mutagenesis is performed by a PCR reaction under
conditions (increased MgCl ² concentration, additional
MnCl ² and unbalanced dNTP ratio) that induce an
increased error-rate of the DNA-polymerase.
JBS dNTP-Mutagenesis Kit
Random Mutagenesis by dNTP Analogs
JBS Error-Prone Kit
Random Mutagenesis by Error-Prone PCR
JBS DNA-Shuffl ing Kit
Random Mutagenesis by DNA Shuffl ing
DNA Mutagenesis Kits
Simply run the PCR protocol provided in the manual and
achieve rates of mutagenesis in the range of 0.6 – 2.0%
in a single PCR step!
Enhanced mutational rate by error-prone PCR compared to standard PCR reactions
Mutagenesis by DNA Shuffl ing
Developed by Stemmer (1994) DNA shuffl ing generates
libraries by random fragmentation of one gene or
a pool of related genes, followed by the reassembly of
the fragments in a self-priming PCR reaction. The rates
of mutagenesis are similar to the error-prone PCR but
DNA shuffl ing allows the recombination of sequences
from different, related genes.
General types of DNA shuffl ing
PP-101 15 reactions 240 €
PP-102 15 reactions 190 €
PP-103 15 reactions 240 €