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Restriction Endonucleases Products (REP) Market - Global Industry Analysis And Forecast 2014 - 2020.pdf

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<strong>REP</strong>ORT DESCRIPTION<br />

<strong>Restriction</strong> enzymes or restriction endonucleases (RE) are the enzymes that are found in the bacteria and<br />

are harvested from them for their use in research and commercial aspects. These enzymes cut the<br />

deoxyribonucleotide (DNA) at specific nucleotide sequences and the biological function of these enzymes<br />

is to protect cells from foreign DNA . Nomeclature for these enzymes is based on the first letter of the<br />

genus followed by first two letters of the species and name of the bacteria of origin. These enzymes find<br />

applications in various research activities involving molecular biology techniques.<br />

Browse Full Report with TOC @ http://www.transparencymarketresearch.com/restrictionendonucleases-products-market.html<br />

For instance, these enzymes are used as key reagents in processes such as restriction fragment length<br />

polymorphism (RFLP), genomic mapping, several recombinant procedures and DNA sequencing. There are<br />

four classes of restriction endonucleases namely types I, II, III and IV of which class II enzymes are the<br />

most popular and more useful as compared to others. The reason being the specific determination of the<br />

DNA sequences and generate termini with 5’ phosphate and 3’ hydroxyl groups and are capable of<br />

recognizing non-palindromic sequences. There has been continuous evolution of the new prototype<br />

activities that render continuous updates about new recognition sites along with the commercial<br />

availability. REBASE is such one database that provides monthly updates about the above mentioned<br />

factors.<br />

Till date more than 10,000 bacteria are screened for the presence of restriction enzymes and currently<br />

there are more than 2,500 restriction enzymes have been discovered along with over 250 distinct<br />

specificities in sequences. The microorganisms that produce RE also produce DNA methyltransferases and<br />

this feature protects their own DNA from cleavage.<br />

These enzymes are used in conventional cloning, deciphering epigenetic modifications, construction of<br />

DNA libraries and in vivo gene editing. The market for restriction endonucleases can be segmented by<br />

various types that are available commercially and the respective application areas. Companies such as<br />

New England Biolabs, Inc. and Thermo Fisher Scientific engage in development and marketing of various

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