BARLEY GENETICS NEWSLETTER - GrainGenes

More documents

Recommendations

Info

Table of Contents Barley Genetics Newsletter, Volume 38, 2008 [Other volumes of the Barley Genetics Newsletter] Information about the Barley Genetics Newsletter, p. i Instructions for contributors, p. ii Memoriam for Jørgan Løhde, p. iii Research Notes Gamma-induced pericentric inversion in barley (Hordeum vulgare L. (.doc, .pdf) 1-3 K.I. Gecheff Yield response to treatment with vesicular-arbuscular myccorrhiza (VAM) in a breeding population of barley (.doc, .pdf) M.C. Therrien A vrs1 mutant in CIho 4196 to facilitate breeding of 6-rowed cultivars with Fusarium Head Blight resistance (.doc, .pdf) C. Boyd, R. Horsley, A. Kleinhofs Worth of Genetic Parameters to Sort out New Elite Barley Lines over Heterogeneous Environments (.doc, .pdf) N. Chand, S.R. Vishwakarama, O.P. Verma, M. Kumar Phenotypic Stability of Elite Barley Lines over Heterogeneous Environments(.doc, .pdf) N. Chand, S.R. Vishwakarama, O.P. Verma, M. Kumar Coordinator's Reports Report of the Workshop on Barley Genetic Linkage Groups, Barley Genome, Genes and Genetic Stocks at the X. International Barley Genetics Symposium. Alexandria, Egypt, April 2008 (.doc, .pdf) U. Lundqvist and A.K. Brantestam 4-6 7-9 10-13 14-17 100-102 Overall Coordinator’s Report (.doc, .pdf) 103-133 U. Lundqvist Table of Barley Genetic Stock Descriptions, Latest Version (.doc, .pdf) 134-164 J.D. Franckowiak, U. Lundqvist Rules for Nomenclature and Gene Symbolization in Barley (.doc, .pdf) 165-170 J.D. Franckowiak, U. Lundqvist
Barley Genetics Newsletter (2008) 38:1-3 Gamma-induced pericentric inversion in barley (Hordeum vulgare L.) K. I. Gecheff, Institute of Genetics, Acad. D. Kostoff Bulgarian Academy of Sciences 1113 Sofia, Bulgaria. The karyotype of barley has been subjected to an extensive experimental reconstruction with respect of both fundamental and applied aspects (for review see Taketa et al. 2003). Reciprocal translocations have been the basic type of structural rearrangements used in this species along these lines. To my knowledge, there are very few attempts to use pericentric inversions as morphological markers for identification of barley chromosomes. For a long time our efforts were aimed at induction by gamma-irradiation of pericentric inversions which affect asymmetrically the morphology of barley chromosomes. Three homozygous lines, namely, I-11, I-44 and I-39, containing such pericentric inversions in chromosomes 2H, 5H and 6H, respectively, were produced so far. The morphology of reconstructed chromosomes is described in this paper. Dry seeds of both standard two-rowed spring barley variety “Freya” and translocation line T- 1586, originating from the same variety were used as experimental material. Doses between 100 and 200 Gy of gamma-rays were applied in order to produce chromosomal rearrangements. Data concerning cytological and selection techniques are given in our previous papers (Gecheff 1989; 1996). The idiograms of chromosomes were constructed following the proposals of Marthe and Künzel (1994). Since pachytene analysis that might reveal a specific loop formation in plant heterozygous for inversions is practically inapplicable in barley, the examination of the morphology and Giemsa N-banding patterns of somatic metaphase chromosomes were the main approach for selection of plants of interest and identification of inversion breakpoints. Later on, the nature of the induced structural rearrangements were confirmed by examination of meiotic metaphase I of F1 hybrid plants in crosses of mutant forms with their parent lines. Pericentric inversion 2H The karyogram analysis of somatic metaphases of mutant line I-11 revealed an alteration in the morphology of chromosome 2H (an apparent increase in the long/short arm ratio) without any change in the morphology of the rest of chromosomes of the karyotype. A comparative analysis of Giemsa N-banded metaphase chromosomes showed an intrachromosomal transfer of the most distal band of the short arm to the long arm of this chromosome, that has evidently resulted from a pericentric inversion embracing a large segment with total length of 65 mGNs. The putative sites of the inversion breakpoints were found to be located within the segments that do not exceed 9 mGNs, as indicated by arrowheads in Fig. 1A. Pericentric inversion 5H This inversion was easily identified because it affects dramatically the standard morphology of chromosome 5H. The evident increase of the size of the satellite up to 36 mGNs was accompanied by corresponding reduction in the size of the long arm of this chromosome. Using the approach mentioned above, the inversion breakpoints are supposed to be localized within the segments that have the same length as the satellite (Fig. 1B). 1
Page 1 and 2: Barley Genetics Newsletter Volume 3
Page 3 and 4: INSTRUCTIONS FOR CONTRIBUTORS TO VO
Page 5 and 6: INSTRUCTIONS FOR CONTRIBUTORS TO VO
Page 7: at RVAU as a lecturer, as well as c
Page 11 and 12: Barley Genetics Newsletter (2008) 3
Page 51 and 52: Table 1. contin. GSHO number Barley
Page 59 and 60:
Barley Genetics Newsletter (2008) 3
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
show all

BARLEY GENETICS NEWSLETTER - GrainGenes

Create successful ePaper yourself

Delete template?

Save as template?