Effect of Isolate of Trichoderma Sp. And Incubation Period to Glucose Production

Effect Of Isolate Of Trichoderma
Variance 0.000 0.000 0.032 0.004 0.003 0.002 0.084
TV.0305
Count 3 3 3 3 3 3 18
Sum 0.083 0.288 1.424 1.993 1.760 1.512 7.060
Average 0.028 0.096 0.475 0.664 0.587 0.504 0.392
Variance 0.000 0.000 0.009 0.001 0.001 0.002 0.064
TV.0710
Count 3 3 3 3 3 3 18
Sum 0.062 0.237 1.187 1.278 1.183 1.082 5.029
Average 0.021 0.079 0.396 0.426 0.394 0.361 0.279
Variance 0.000 0.000 0.000 0.000 0.000 0.000 0.029
Total
Count 9 9 9 9 9 9
Sum 0.239 0.841 4.336 5.531 4.901 4.214
Average 0.027 0.093 0.482 0.615 0.545 0.468
Variance 0.000 0.000 0.016 0.023 0.014 0.008
Table 3 shows that the test is performed with three repetitions. The average total value of glucose for
each isolate TV.0209 has 0443 (3,616 ppm) with variance 0084, TV.0305 has 0392 (3,253 ppm) with variance
0.064 and TV.0710 has 0279 (2,448 ppm) with variance 0.029.
The total average results show that isolates TV.0209 have a total average of the highest compared to TV.0305,
while TV.0710 has a total average of the lowest. The same pattern occurs in which the variance TV.0209 has
the highest value compared to TV.0305 and TV.0710. Analysis of total isolates TV.0209, TV.0305, and
TV.0710 obtained total value of the average of 0.468 and 0.008 variance.
Table 4. Test Results of Two-Way Anova
Source of Variation SS df MS F P-value F crit
Sample 0.252 2 0.126 43.160 0.000 3.259
Columns 2.762 5 0.552 188.944 0.000 2.477
Interaction 0.131 10 0.013 4.493 0.000 2.106
Within 0.105 36 0.003
Total 3.250 53
To determine the effect of incubation time isolates against statistically performed using two-way
anova with the following results. Hypothesis column in Table 3, show the p value less than 0.05, then the
decision to accept H 1 and reject H 0 . Furthermore, it appears the value of F is higher than F crit . In other words,
F calculated > F table . It means that there is a significant difference between the average value calculated from
categories of incubation period. Each isolate that is different glucose results in any period of incubation. Each
incubation period applied for a 10 days, Trichoderma isolates give results significantly different values. This
difference indicates that production of glucose produced by cellulase enzyme activity which produced by each
isolate. The results of this study reinforce the research data conducted by Awojobi et al. [12] who did research
some Trichoderma isolates within 5 to 11 days incubation. Research results mention that there are significant
differences between the amount of cellulase with incubation period.
Hypotheses rows showed the p value less than 0.05, then the decision to accept H 1 and reject H 0 . It
appears the value of F > F crit means that there is a significant difference between the average value calculated
from categories of isolates. Each isolate TV.0209, TV.0305, and TV.0710 have significantly different values in
producing glucose at any incubation period. This data is confirmed by the results of research Singh et al. [13]
which states that the ability of each species Trichoderma growth generally depend on a variety of organic
materials applied in degrading complex compounds into simpler compounds. The large amount of degraded
compounds associated with the incubation period applied in its growth. Therefore, each species Trichoderma
can be determined the optimum conditions that exist in the resulting product.
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