EPITHELIUM AND GLANDS
Epithelium and glands - PEER
Epithelium and glands - PEER
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Slide 33: Kidney (PAS/hematoxylin)<br />
Periodic acid – Schiff<br />
• Used to visualize: glycogen, glycoproteins, and glycosaminoglycans<br />
• Steps in PAS staining<br />
1. Periodic acid oxidizes 1,2-glycols to aldehydes<br />
2. Schiff’s reagent colors the aldehyde groups (pink to magenta)<br />
Brush border<br />
Basement<br />
membrane<br />
The brush border is composed of<br />
numerous microvilli which function<br />
to increase surface area for<br />
absorption of nutrient material and<br />
fluid.<br />
The structure on the brush border<br />
that stains with periodic-acid Schiff<br />
(PAS) stain is the glycocalyx.
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