LOEWE Product Information 2017

LOEWE ®
Solutions for Plant Disease Diagnostics
Product
Information
2017
Molecular Diagnostics
Rapid Tests
ELISA Products
Immuno Fluorescence

Dear Partners and Colleagues,
we invite you to browse our new Catalog for Plant Disease Diagnostics for the year 2017.
Although ELISA tests still constitute the majority of our products, our objective is to present to you a choice of
high-quality diagnostic tools, that will satisfy your specific needs for pathogen testing.
These tools include:
• A range of more almost 150 ELISA antisera for the detection of plant viruses, bacteria, and fungi
• PCR complete reaction kits and RNA PCR complete reaction kits for easy and convenient
molecular detection of plant bacteria and RNA-Viruses
• Antisera and supplements for Immuno Fluorescence assays
• LOEWE®FAST rapid tests for fast and easy assessment of suspicious plant material
Our focus remains on the expansion of our range of molecular diagnostic kits for PCR detection of bacteria,
phytoplasms, fungi, and RNA-Viruses. Thanks to our busy R&D team, this product line now already
encompasses close to 30 pathogens.
NEW: As an additional service for you, we are now also providing kits for easy extraction of high quality DNA
and RNA, which are provided by our German partner Analytic Jena AG. A detailed description of our
molecular diagnostics can be found on pages 3 and 4.
Contact and Order Information
Direct and immediate contact to our customers is one of our main goals. Our products can be ordered directly
from LOEWE® Biochemica GmbH, Sauerlach, Germany. For ordering please contact us by e-mail to
sales@loewe-info.com or register with our web-shop at: www.loewe-info.com
In addition our products are available through a worldwide net of authorized distributors. Please visit our
website: www.loewe-info.com/delivery.html for ordering information in your region.
The team of LOEWE® Biochemica is looking forward to help you with any questions and inquiries!
Sincerely,
Dr. Caroline Freye-Minks and Dr. Renate Loewe
LOEWE ®
Mühlweg 2a
82054 Sauerlach
Germany
Phone: (+49) 8104 61620
Fax: : (+49) 8104 61648
E-Mail: sales@loewe-info.com

LOEWE ®
Content
Product Information Page 3 - 5
NEW: Molecular Extraction Kits Page 6
One-Tube One-Step RNA PCR - Viruses Page 6 - 7
DNA PCR Products Page 8 - 10
DNA PCR Products – Phytoplasma Page 8
DNA PCR Products – Bacteria Page 9 - 10
DNA PCR Products – Fungi Page 10
LOEWE ® FAST Rapid Tests Page 11 - 13
ELISA Products Page 14 - 55
ELISA Products – Viruses Page 14 - 47
ELISA Products– Bacteria Page 48 - 54
ELISA Products - Fungi Page 54 - 55
Immuno Fluorescence Products Page 55 - 57
Immuno Fluorescence - Antisera Page 55 - 57
Immuno Fluorescence - Secondary Antibodies Page 57
Immuno Fluorescence - Disposables Page 57
ELISA Chemicals Page 57 - 58
ELISA Disposables Page 58
AGB - General Terms for Sales and Delivery
Appendix

LOEWE ® Product Information
One-Tube One-Step RNA PCR - Viruses
The PCR technique allows specific and sensitive detection of plant pathogens, when serological
reagents are not available or a second method is needed for confirmation of test results. We are
offering a continously growing range of RNA PCR complete reaction kits for reverse transcriptase PCR
of RNA plant viruses.
Kits Content:
Premix (Primers, dNTPs)
DNA-Polymerase
Reverse Transcriptase (M-MLV, RNase H-)
Reaction Buffer
PCR-grade Water
Dithiothreitol (DTT)
Positive Control (DNA based)
Negative Control (DNA based)
All components have been optimized regarding concentrations
and reaction conditions for reliable and repeatable test results
and are accompanied by a comprehensive test protocol.
The reagents are mixed in one tube on the bench top and the
reverse transpcription and cDNA amplification take place in one
reaction step without additional pipetting step, minimalizing the
risk of cross contamination.
Enzymes are thermostable and kits can be shipped at room
temperature without the need of dry-ice!
Procedure:
• extract virus RNA from your sample with your favorite method or our new extraction kits (see below)
• run the reverse transcription and cDNA amplification in one tube and one step
• run an agarose gel and analyse results
Virus-RNA from infected plant
1. Reverse Transcription
2. cDNA Amplification
3 Hours Amplified cDNA
Detection
in agarose gel
NEW: Molecular Extraction Kits *
DNA Extraction Kit for Plants
Kits are specially optimized for the extraction
and purification of DNA from plant material
(leaves, stems, roots, flowers). The process
involves a prefiltering step, which effectively
removes non-lysed plant material. Subsequent
binding with a novel binding buffer to a spinfilter
column and elution to a tube enables fast
and easy purification of DNA, which can be used
directly in downstream applications or stored
for later use. One kit is sufficient for 50 DNA
extractions and contains:
lysis buffers
precipitation buffer
binding and washing solution, proteinase K
prefilters, spin filters with receiver and
elution tubes and manual
RNA Extraction Kit for Plants
Kits are specially optimized for the extraction
and purification of RNA from plant material
(leaves, stems, roots, flowers). Due to its
chemistry, lysis is highly efficient and two lysis
buffers are included in the kit, in order to enable
processing of a wide range of different plant
matrices. Within only 30 minutes, total RNA of
excellent quality can be extracted, which can be
used directly in downstream applications. One
kit is sufficient for 50 RNA extractions and
contains:
2 different lysis buffers
washing solutions
RNase-free water
spin filters with receiver and elution tubes
manual
* Kits and descriptions provided by our German partner Analytik Jena AG

LOEWE ® Product Information
DNA PCR Products
Our DNA PCR kits and sets for the detection of bacteria, fungi, and phytoplasms are the perfect tool to
establish PCR analysis in your lab! Your sample DNA is subjected to a pre-tested and evaluated PCR system,
without the hassle of optimizing reaction- and cycle conditions.
DNA PCR Sets
The PCR sets contain a premix, including pathogen specific primers and dNTPs for 100 PCR reactions and
positive and negative controls for 10 reactions. All components are pre-tested and each product is
accompanied by a lot-specific certificate and a working protocol.
DNA PCR Complete Reaction Kits
Included are a vaildated premix with pathogen specific primers
and dNTPs, DNA-based positive and negative controls,
thermostable DNA-Polymerase and a detailed protocol. The kits
can be shipped at room temperature.
Complete PCR Reaction Kits
Premix (Primers, dNTPs)
DNA-Polymerase
10 x Reaction Buffer
PCR-grade Water
Positive Control (DNA based)
Negative Control (DNA based)
LOEWE®FAST Rapid Tests
The LOEWE®FAST rapid test series is designed for reliable and specific
detection of plant pathogens within minutes. You will be able to assess
suspicious plant material in the field or greenhouse without the need of
an analytical laboratory.
Available for a series of viruses and bacteria like the quarantine pests
C.m.s and Ralstonia solanacearum.
Fast - easy - on the spot
Specific detection of plant pathogens within minutes
No laboratory needed
A LOEWE®FAST basic kit includes:
• 5 single-use test cassettes
• 5 pre-filled tubes with sample buffer
• 5 single-use pipettes
Visit our
You-Tube
Channel for
a ‘How-todo
Video’
Immuno Fluorescence Products
Especially if testing a large number of samples, immuno fluorescence
still is a very popular and straightforward technique. We are offering a
wide range of polyclonal antisera against plant bacteria from goat and
rabbit, secondary fluorescence labelled antibodies, as well as multiwell
slides and cover slides for immuno fluorescence studies:
Antisera and Controls
Fluorescence labelled Secondary Antibodies (FITC or Cy3 TM )
Multiwell Slides
Cover Slides

LOEWE ® Product Information
ELISA Products
ELISA (Enzyme-linked Immunosorbent Assay) is the most common test method for the detection of plant
pathogens, like viruses and bacteria. LOEWE® offers a range of 150 high quality ELISA products for specific and
sensitive detection. ELISA reagents can be supplied as sets or complete kits. All necessary reagents, buffers
and plates are also available separately (see pages 57-58, ELISA Chemicals and Disposables).
ELISA Sets
contain Coating-IgG and AP-Conjugate and are supplied as 100, 300, 500,
and for many pathogens as 1000 and 5000 test units.
One test corresponds to one well in the ELISA plate. The reagents are
optimized and evaluated for a test volume of 200 µl, if not indicated
otherwise in the test manual, ensuring highest accuracy, repeatability and
sensitivity.
ELISA Complete Kits
are available for 96, 480, and for many pathogens as 960 test kits. The kits
contain all ELISA reagents, positive and negative controls, buffers and ELISA
plates or modules to perform the ELISA assay.
Components 96 test kits 480 test kits 960 test kits
Antibody (IgG) 0.1 ml 0.5 ml 1.0 ml
Antibody-AP-conjugate 0.1 ml 0.5 ml 1.0 ml
Positive Control 10 tests 10 tests 20 tests
Negative Control 10 tests 10 tests 20 tests
Coating Buffer 1 liter 1 liter 1 liter
Wash Buffer 1 x 5 liter 1 x 5 liter 2 x 5 liter
Conjugate/Sample Buffer 1 x 1 liter 2 x 1 liter 4 x 1 liter
Substrate Buffer (5x) 1 x 25 ml 1 x 25 ml 2 x 25 ml
Substrate Tablets 4 x 5 mg 5 x 20 mg 10 x 20 mg
Tween 20 10 ml 10 ml 10 ml
High-binding ELISA plates 12 Modules à 8 wells 5 plates 10 plates
Sealing Cover 1 sheet 5 sheets 10 sheets
ELISA Positive and Negative Controls
Negative controls are made from a healthy host plant and are tested for the absence of the respective
pathogen. They are lyophilized and sufficient for 10 test wells.
Positive controls are made from infected plant material or bacterial cultures, if not stated otherwise. They are
lyophilized and sufficient for 10 test wells. In case of pathogens, that are listed as quarantine pests,
inactivated positive controls are supplied, which are additionally tested for the absence of infectivity.
Unspecific non-pathogenic ‘method’ controls are sold for some restricted pathogens.

www.loewe-info.com
LOEWE® Biochemica GmbH
Mühlweg 2a
82054 Sauerlach
Germany
Phone: +49 (0) 810461620
Fax: +49 (0) 8104 61648
E-mail: sales@loewe-info.com
Web: www.loewe-info.com
© LOEWE®Biochemica GmbH 2017
valid from 01.01.2017