Immunofluorescence Assays Market Size will Grow at a Robust Pace through 2025

Immunofluorescence is the specific antigen and antibody reaction where the antibodies are labeled
with a fluorescent dye and the antigen-antibody complex is visualized using fluorescent (UV)
microscope. Some of the commonly used fluorochromes include Acridine Orange, Lissamine,
Rhodamine, and Calcofluor white. Various factors determine the type of immunofluorescence assay
used such as time consumed, cost, complexity, flexibility, sensitivity, cross reactivity and many
others. It is used in all disciplines of biology including medicine for diagnostics and research.
Immunofluorescence assays are used to detect specific proteins in cells that may be in specimen, in
culture, in tissues, on microbeads and microarrays, etc.
Two types of immunofluorescence assays are usually performed: Direct and Indirect
immunofluorescence. Direct immunofluorescence used less often as the antibody against the
molecule of interest is conjugated chemically to a fluorescent dye, so to spot every antigen, the
specific antibody will have to be conjugated with FITC. In indirect immunofluorescence assay, the
antibody specific for the molecule of interest i.e. the primary antibody is not labeled, and the second
anti-immunoglobulin antibody focused toward the first antibody i.e. the secondary antibody is
tagged with some fluorescent dye. Indirect fluorescence is used more commonly as the tagged
secondary antibody and can be used to detect many different antigens. However, the primary
antibody will have to be specific for the antigen to be detected.
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The fluorescence can be read as a qualitative result or quantitative result using fluorescence
microscopy. The fluorescence can also be quantified using a flow cytometer, array scanner or
automated imaging instrument.
Immunofluorescence assays Market: Drivers & Restraints
The improved versions of kits and reagents increases its demand. Increase in the number of cancer
incidences and various infectious diseases rising the demand. Investments in research by various
governmental and non-governmental organizations is upsurging the demand for these kits. Some of
the restraining factors include the potential of cross reactivity, lower signal and higher costs of kits.
Introduction of more safer and cost effective tests which diverting the preference of clinicians and
researchers from immunofluorescence assays.
Immunofluorescence assays has demand in both the research as well as clinical diagnostics
purposes. Market includes various antibodies, regents, kits, microscopes, labeling dyes etc.
Immunofluorescence preparations can be analyzed by various microscopy techniques such as
Epifluorescence, TIRF, and GSDIM depending on the type of application it is used for. Thus, with
the increase in immunofluorescence assay, need of fluorescence microscopy increases.
Technological development, expansion in the field of immunofluorescence assays, commoditization
of products and strong network of distributors at global and regional level also contributing to the

growth of market.
A geographic condition regarding the Immunofluorescence assays market, it has been segmented
into five key regions: North America, Latin America, Europe, Asia-Pacific and the Middle East &
Africa. High incidences of chronic and infectious diseases, laboratory automation, improved
technology and favorable reimbursement scenario are some of the factors which have led North
America dominating the immunofluorescence assay market. It is followed by Europe and most of
the market in Germany. Asia Pacific is increasing in the immunofluorescence assay market because
of the increasing number of market players and rising demand of improved products in diagnostics
as well as academic purpose. India, China and Japan are the countries with highest growth rate in
Asia Pacific region and increases the chances of business opportunities in these sectors.
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Some of the global key players in the Immunofluorescence assays market for manufacturing kits
and reagents for diagnosis are Thermofischer, Biorad, Universla Biologicals, Perkin Elmer,
Maxvision Biosciences Inc., Euro Diagnostica, Sigma Aldrich and others. Some companies
involves in the fluorescence microscope market includes Olympus lifescience, Leica Microsystems,
EuroImmun AG.

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