annual meeting of esna - pribina.savba.sk - SAV

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Genetic transformation of conifers via particle bombardment and Agrobacterium tumefaciens Terézia Salaj, Jana Moravčíková, Božena Vooková, Ján Salaj Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic In recent years, genetic transformation protocols have been developed for several conifer species. Due to high regeneration capacity of the conifer embryogenic cultures they are characterised as ideal tissues for transgenosis. The most important feature of the embryogenic tissues is the presence of bipolar structures – somatic embryos that are capable of development and whole plant (trees) regeneration. Somatic embryogenesis has been initiated in Pinus nigra Arn. and hybrid firs (Abies alba x A. cephalonica and Abies alba x A. numidica). The long-term storage of tissues occurred on the solid culture media. The introduced DNA consisted of the uidA reporter gene under the control of double CaMV 35S promoter and the nptII selection gene. In Pinus nigra (cell line E104) stable transformation has been achieved via particle bombardment. The integration of foreign genes has been confirmed histochemically as well as by PCR analysis. Similarly, in hybrid firs (Abies alba x A. cephalonica, cell lines AC2 and AC78, and Abies alba x A. numidica, cell line AN72) transgenic tissues have been obtained by genetic transformation using Agrobacterium tumefaciens. The PCR-positive embryogenic tissues produced small plantlets and out of 36 analysed emblings in 11 of them the presence of reporter uidA as well as nptII selection genes was confirmed. Acknowledgement: The work was granted by MVTS - COST FP 0905 (Slovak Academy of Sciences) project. 130
Topic III MOLECULAR MARKERS IN PLANT BREEDING 131
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41 st ANNUAL MEETING OF ESNA (Europ
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CONTENT Invited lectures 11 Crop yi
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Integrated effects of drought and t
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Technology of growing grain Pashtet
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SESSION III WORKSHOP - RECENT ADVAN
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INVITED LECTURES 11
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References 1. Strasser, R.J. The gr
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Chlorophyll fluorescence: past, rec
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Toward a sustainable agriculture St
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Plant regeneration in vitro: from a
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3. It is difficult to predict the i
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SESSION I ECOLOGY AND CROP PRODUCTI
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Plant hormone dynamics during the c
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The interaction of the heavy metals
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The methodological approach for the
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Influence of clonal rootstocks on t
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Differences in response of some Tun
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Nitric oxide metabolism in senescin
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Impact of potassium on some physiol
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Topic II ECOLOGY OF CROP PRODUCTION
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Relationship between ion leakage an
Page 43 and 44:
High power microwaves and temperatu
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Tolerance of four mutant lines of b
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Spectral dependence of pigments in
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Integrated effects of drought and t
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Morphological, physiological and bi
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Progressive reduction of key Brassi
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The impact of LEDs spectrum on grow
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The altitudinal gradient influence
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Effect of shading on the efficiency
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The photosynthetic heat tolerance a
Page 63 and 64:
Chlorophyll fluorescence- and assim
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Evaluation of some Romanian corn (Z
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Differences of endogenous phytohorm
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The resistance breeding to broomrap
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Response of surface irrigated wheat
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Alleviation of the adverse effects
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Biological diversity of wheat, impr
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Topic I CROP MANAGEMENT FOR FOOD, F
Page 79 and 80: Nitrogen deficiency detection and f
Page 81 and 82: Technology of growing grain-crops V
Page 83 and 84: Biomass yield of giant reed (Arundo
Page 85 and 86: Effect of controlled traffic farmin
Page 87 and 88: The influence of white plastic mulc
Page 89 and 90: Preliminary risk analysis and asses
Page 91 and 92: Observation regarding the efficacy
Page 93 and 94: The effect of phytoprotection progr
Page 95 and 96: Diversity and dominance in the weed
Page 97 and 98: A long-term evaluation of recycled
Page 99 and 100: An attempt of thermography applicat
Page 101 and 102: Effect of ascorbic acid in ovo admi
Page 103 and 104: Influence of microbial preparations
Page 105 and 106: Topic III FOOD PRODUCTION, QUALITY,
Page 107 and 108: Radiocaesium in selected forest and
Page 109 and 110: Method for species identification o
Page 111 and 112: Influence of fertilization system o
Page 113 and 114: Influence of storage conditions on
Page 115 and 116: SESSION III WORKSHOP - RECENT ADVAN
Page 117 and 118: Studies for the micropropagation of
Page 119 and 120: The importance of in vitro cultures
Page 121 and 122: Micropropagation and adventitious r
Page 123 and 124: Proteomic analysis of somatic embry
Page 125 and 126: The share of androdiploids among re
Page 127 and 128: Genetic transformation of Rubus fru
Page 129: Influence of topology of heterologo
Page 133 and 134: Determination of genetic diversity
Page 135 and 136: Topic IV APPLIED PLANT BIOTECHNOLOG
Page 137 and 138: Results of in vitro thermotherapy a
Page 139 and 140: Growth and monoterpene production i
Page 141 and 142: Phytoremediation strategies applied
Page 143 and 144: Evaluation of nontransgenic resista
Page 145 and 146: Evaluation of currant perspective v
Page 147 and 148: LIST OF PARTICIPANTS 147
Page 149 and 150: prochazk@mendelu.cz Dagmar PROCHÁZ
Page 151 and 152: Karolina GŁODEK University of Agri
Page 153 and 154: Cluj-Napoca alexafinarul@gmail.com
Page 155 and 156: Terézia SALAJ Institute of Plant G
Page 157 and 158: PARTNERS 157
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