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Nanopore Sequencing:An Introduction
BOOK DETAIL
File Size: 7320 KB Print Length: 216 pages Publisher: World Scientific Publishing Company (April 5, 2019)
Publication Date: April 5, 2019 Sold by: Amazon.com Services LLC Language: English ASIN:
B07R8D148T Text-to-Speech: Enabled
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Book Description
This is an introductory text and laboratory manual to be used primarily in undergraduate courses.
It is also useful for graduate students and research scientists who require an introduction to the
theory and methods of nanopore sequencing. The book has clear explanations of the principles of
this emerging technology, together with instructional material written by experts that describes
how to use a MinION nanopore instrument for sequencing in research or the classroom. At
Harvard University the book serves as a textbook and lab manual for a university laboratory
course designed to intensify the intellectual experience of incoming undergraduates while
exploring biology as a field of concentration. Nanopore sequencing is an ideal topic as a path to
encourage students about the range of courses they will take in Biology by pre-emptively
addressing the complaint about having to take a course in Physics or Maths while majoring in
Biology. The book addresses this complaint by concretely demonstrating the range of topics —
from electricity to biochemistry, protein structure, molecular engineering, and informatics — that
a student will have to master in subsequent courses if he or she is to become a scientist who truly
understands what his or her biology instrument is measuring when investigating biological
phenomena. Contents:The Development of Nanopore Sequencing (Daniel Branton and David
Deamer)Ionic Currents (Stephen Fleming)Lipids and Nanopores (David W Deamer)Nanopore
Structure, Assembly, and Sensing (Daniel Branton)Helicases and DNA Motor Proteins (Alicia K
Byrd and Kevin D Raney)Development of Multipore Sequencing Instruments (James
Clarke)DNA Extraction Strategies for Nanopore Sequencing (Joshua Quick and Nicholas J
Loman)Molecular Engineering DNA and RNA for Nanopore Sequencing (Andrew J
Heron)Bioinformatic Analysis of Nanopore Data (Miten Jain)Two Suggested Student Laboratory
Exercises (Daniel Branton and Stephen Fleming) Readership: Undergraduate students, graduate
students and research scientists who require and introduction to the theory and methods of
nanopore sequencing, bioinformatics, clinical technicians, and other biologists who have had no
training in nanopore sequencing.

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