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Molecular Cloning: A Laboratory Manual (Fourth Edition):
Three-volume set
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business account Paperback: 2028 pages Publisher: Cold Spring Harbor Laboratory Press; 4th edition (June
15, 2012) Language: English ISBN-10: 1936113422 Product Dimensions: 11.3 x 3.9 x 9.2 inches Shipping
Weight: 13 pounds (View shipping rates and policies) Customer Reviews: 4.0 out of 5 stars25 customer
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Book Description
Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30
years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition,
by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI
investigator Michael Green, preserves the highly praised detail and clarity of previous editions
and includes specific chapters and protocols commissioned for the book from expert practitioners
at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory,
Washington University, and other leading institutions. The theoretical and historical
underpinnings of techniques are prominent features of the presentation throughout, information
that does much to help trouble-shoot experimental problems. For the fourth edition of this classic
work, the content has been entirely recast to include nucleic-acid based methods selected as the
most widely used and valuable in molecular and cellular biology laboratories. Core chapters from
the third edition have been revised to feature current strategies and approaches to the preparation
and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12
new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and
manipulated, and how data generation and analysis can be handled. The new content includes
methods for studying interactions between cellular components, such as microarrays, nextgeneration
sequencing technologies, RNA interference, and epigenetic analysis using DNA
methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data
produced by these techniques, a bioinformatics chapter describes the use of analytical tools for
comparing sequences of genes and proteins and identifying common expression patterns among
sets of genes. Please take a look at our book preview site at molecularcloning.com. Building on
thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new
gold standard--the one indispensable molecular biology laboratory manual and reference source.

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